MS001149 (gene) Bitter gourd (TR) v1

BLAST of MS001149 vs. NCBI nr
Match: XP_022131303.1 (kinesin-like protein KIN-4C [Momordica charantia])

HSP 1 Score: 750.0 bits (1935), Expect = 1.2e-212
Identity = 410/437 (93.82%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKASRQSRQTGEADVSSSPVTSATDNKDYE RVQELERQNEAFQKEIKDLKDRLEIASS
Sbjct: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYENRVQELERQNEAFQKEIKDLKDRLEIASS 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLDVLGD                        QVTELKKKLDAQSQ
Sbjct: 61  SAANSAKKLREGYIQKLDVLGD------------------------QVTELKKKLDAQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LSIRRQKGEGATKQTDLEITSLKAQK VQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR
Sbjct: 121 LSIRRQKGEGATKQTDLEITSLKAQK-VQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHGINSTQY 240
           RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHGINSTQY
Sbjct: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHGINSTQY 240

Query: 241 VENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENNQ 300
           VENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENNQ
Sbjct: 241 VENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENNQ 300

Query: 301 DVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEHG 360
           DVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKD+SGTTSASVGSNNLFEQFDTAGEEHG
Sbjct: 301 DVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDDSGTTSASVGSNNLFEQFDTAGEEHG 360

Query: 361 QGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGETI 420
           QGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGETI
Sbjct: 361 QGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGETI 412

Query: 421 HEFGPPMKPLSDIKNVL 438
           HEFGPPMKPLSDIKNVL
Sbjct: 421 HEFGPPMKPLSDIKNVL 412

BLAST of MS001149 vs. NCBI nr
Match: XP_038884450.1 (kinesin-like protein KIN-4C [Benincasa hispida])

HSP 1 Score: 601.7 bits (1550), Expect = 5.2e-168
Identity = 341/443 (76.98%), Postives = 374/443 (84.42%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRK +RQS+ +GEADVSSS VTSATD+KDYEKR++EL R+NEAFQKEIKDLKD+LE+A+S
Sbjct: 1   MRKTTRQSKHSGEADVSSSSVTSATDSKDYEKRIRELTRENEAFQKEIKDLKDKLEVATS 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKK+REGYIQKLDVL D                        QVTELKKKLD QSQ
Sbjct: 61  SAANSAKKIREGYIQKLDVLED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS RRQ+G+ ATK +DLEI SLKAQK VQLQCKMKLESVQFRL KGSLEKEILQLKR+NR
Sbjct: 121 LSTRRQRGDEATKHSDLEIMSLKAQK-VQLQCKMKLESVQFRLYKGSLEKEILQLKRENR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKA--GAKH-GINS 240
           RNE++MN LITSNQRLKMVLQRKTEEASEV KR+RNLLESRR SAQR+A  GAKH  I S
Sbjct: 181 RNEHEMNKLITSNQRLKMVLQRKTEEASEVMKRMRNLLESRRTSAQRRAGGGAKHRNITS 240

Query: 241 TQYVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILE 300
           TQYVENEFEVTARLHELCSQYEH+IEEKDKEIA+L+EEA+ L QE SGY SQ+TDVNILE
Sbjct: 241 TQYVENEFEVTARLHELCSQYEHQIEEKDKEIAKLQEEADVLKQERSGYPSQETDVNILE 300

Query: 301 NNQDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGE 360
           NNQD+NELKEQMVVLS L NQM+IQK   TH+D SKD+S  TSASVGSNNLFEQFDTAGE
Sbjct: 301 NNQDMNELKEQMVVLSSLFNQMQIQKVNKTHKDTSKDDSAQTSASVGSNNLFEQFDTAGE 360

Query: 361 EHGQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKC---SNRYE 420
           +  QGF++VTKRT KAECCSCSKKSLCKT KCKCRSTG SCG  CGCA+GKC   SNR E
Sbjct: 361 DQ-QGFDVVTKRTAKAECCSCSKKSLCKTTKCKCRSTGRSCGTLCGCALGKCTNRSNRDE 417

Query: 421 KPGETIHEFGPPMKPLSDIKNVL 438
           KPGE I+ FGPPMKPLSDIKNVL
Sbjct: 421 KPGEMINVFGPPMKPLSDIKNVL 417

BLAST of MS001149 vs. NCBI nr
Match: XP_023001861.1 (kinesin-like protein KIN-4C [Cucurbita maxima])

HSP 1 Score: 590.5 bits (1521), Expect = 1.2e-164
Identity = 329/438 (75.11%), Postives = 364/438 (83.11%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKASR ++Q GEAD S S VTSATD++DYEKR+QELER+N+AFQKEIKDLKDRLEIASS
Sbjct: 1   MRKASRPTKQKGEADASPSSVTSATDSRDYEKRIQELERENKAFQKEIKDLKDRLEIASS 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLD+L D                        QVTELKKKLD QSQ
Sbjct: 61  SAANSAKKLREGYIQKLDILED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS RR KG+GATKQTD EI SLKAQK V LQCKMKLESVQFRLCKGSLEKE+LQLKR+NR
Sbjct: 121 LSTRRPKGDGATKQTDFEIMSLKAQK-VHLQCKMKLESVQFRLCKGSLEKEVLQLKRENR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAK-HGINSTQ 240
           RNE++MN L TSNQRLKMVLQRKTEEASE+TK+IRNLLESRR SAQR+AGA+   + S+Q
Sbjct: 181 RNEHEMNKLRTSNQRLKMVLQRKTEEASEITKKIRNLLESRRTSAQRRAGARQENVTSSQ 240

Query: 241 YVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENN 300
            VENEFEVTA LHELCSQYEH+IEEKDKEIA+L+EE +AL QE +GY S +TD N+L NN
Sbjct: 241 SVENEFEVTASLHELCSQYEHQIEEKDKEIAKLQEEVDALKQEETGYQSLETD-NVLGNN 300

Query: 301 QDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEH 360
           QD  ELKEQMV+LSGLVNQMRIQKF HT +  SKD+SG +SAS GSNNLFEQF  AGEEH
Sbjct: 301 QDAAELKEQMVILSGLVNQMRIQKFNHTQKGESKDDSGPSSASNGSNNLFEQFAIAGEEH 360

Query: 361 GQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGET 420
            +GF + TKR VKAECCSCSKKSLCKT KCKCRSTGSSCG+SCGCA GKCSNR EKP E 
Sbjct: 361 WRGFNVATKRAVKAECCSCSKKSLCKTTKCKCRSTGSSCGSSCGCAHGKCSNRDEKPSEM 412

Query: 421 IHEFGPPMKPLSDIKNVL 438
           + EFGPPM PLSDIKN+L
Sbjct: 421 VDEFGPPMNPLSDIKNLL 412

BLAST of MS001149 vs. NCBI nr
Match: XP_023536988.1 (kinesin-like protein KIN-4C [Cucurbita pepo subsp. pepo])

HSP 1 Score: 589.7 bits (1519), Expect = 2.0e-164
Identity = 331/438 (75.57%), Postives = 365/438 (83.33%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKASRQ++Q GEAD SSS VTSATD++DYEKR+QELER+N+AFQKEIKDLKDRLEIASS
Sbjct: 1   MRKASRQTKQKGEADASSSSVTSATDSRDYEKRIQELERENKAFQKEIKDLKDRLEIASS 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           S ANSAKKLREGYIQKLDVL D                        QVTELKKKLD QSQ
Sbjct: 61  SDANSAKKLREGYIQKLDVLED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS RR KG+GATKQTD EI SLKAQK VQLQCKMKLESVQFRLCKGSLEKE+LQLKR+NR
Sbjct: 121 LSTRRPKGDGATKQTDFEIMSLKAQK-VQLQCKMKLESVQFRLCKGSLEKEVLQLKRENR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAK-HGINSTQ 240
           RNE++MN L TSNQRLKMVLQRKTEEASE+TK+IRNLLESRR SAQR+ GA+   + S+Q
Sbjct: 181 RNEHEMNKLRTSNQRLKMVLQRKTEEASEITKKIRNLLESRRTSAQRRTGARQENVTSSQ 240

Query: 241 YVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENN 300
            VENEFEVTA LHELCSQYEH+IEEKDKEIA+L+EEA+AL QE + Y S +TD N+L  N
Sbjct: 241 SVENEFEVTASLHELCSQYEHQIEEKDKEIAKLQEEADALKQEQTWYQSLETD-NVLGIN 300

Query: 301 QDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEH 360
           QDV ELKEQMV+LSGLVNQMRIQKF HT +D SKD+SG +SAS GSNNLFEQF  AGEEH
Sbjct: 301 QDVAELKEQMVILSGLVNQMRIQKFNHTQKDESKDDSGPSSASNGSNNLFEQFAIAGEEH 360

Query: 361 GQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGET 420
             GF + TKRTVKAECCSCSKKSLCKT KCKCRSTGSSCG+SCGCA GKCSNR +KP E 
Sbjct: 361 QHGFNVATKRTVKAECCSCSKKSLCKTTKCKCRSTGSSCGSSCGCARGKCSNRDDKPSEM 412

Query: 421 IHEFGPPMKPLSDIKNVL 438
           + EFGP M PLSDIKN+L
Sbjct: 421 VDEFGPQMNPLSDIKNLL 412

BLAST of MS001149 vs. NCBI nr
Match: XP_022997164.1 (kinesin-like protein KIN-4C isoform X1 [Cucurbita maxima])

HSP 1 Score: 585.9 bits (1509), Expect = 2.9e-163
Identity = 335/437 (76.66%), Postives = 365/437 (83.52%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKA+RQS++TGEADVSSS VTSATD+KDYEKR++ELE +NEAFQKEIKDLK+RLE AS 
Sbjct: 1   MRKATRQSKKTGEADVSSSSVTSATDSKDYEKRIRELESENEAFQKEIKDLKNRLENASP 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLDVL D                        QVTELKKKLD QSQ
Sbjct: 61  SAANSAKKLREGYIQKLDVLED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS R +KG+G +KQTDLEI SLKAQK VQLQCKMKLESVQFRL KGSLEKE+LQLKR+ R
Sbjct: 121 LSTRWKKGDGVSKQTDLEIMSLKAQK-VQLQCKMKLESVQFRLYKGSLEKEVLQLKREKR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHG-INSTQ 240
           RNE++MN L+TSNQRLKMVLQRKTEEASEVTKRIRNLLESRR SAQR+AGAKHG I STQ
Sbjct: 181 RNEHEMNKLLTSNQRLKMVLQRKTEEASEVTKRIRNLLESRRTSAQRRAGAKHGNITSTQ 240

Query: 241 YVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENN 300
           YVENEFEVT RLHELCSQYEH IEEKDKEIA+L+EEA+AL QE SGY SQ TD NILENN
Sbjct: 241 YVENEFEVTVRLHELCSQYEHLIEEKDKEIAKLQEEADALKQEKSGYPSQDTDDNILENN 300

Query: 301 QDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEH 360
           QD+NELKEQMV+LSGL NQMRIQKF HT +D SKD S  TS S GSNNL E+FD+AGEEH
Sbjct: 301 QDMNELKEQMVMLSGLFNQMRIQKFNHTPKDASKDVSDQTSPSFGSNNLCERFDSAGEEH 360

Query: 361 GQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGET 420
            Q  + V K TVK ECCSCSKKSLCKT KC+CRSTG SCG SCGCA+GKCSNRYEKPGE 
Sbjct: 361 RQ--DNVKKITVKEECCSCSKKSLCKTTKCQCRSTGRSCGTSCGCALGKCSNRYEKPGEM 408

Query: 421 IHEFGPPMKPLSDIKNV 437
           I+  G P+KPLSDIKNV
Sbjct: 421 IN--GQPLKPLSDIKNV 408

BLAST of MS001149 vs. ExPASy Swiss-Prot
Match: F4K0J3 (Kinesin-like protein KIN-4C OS=Arabidopsis thaliana OX=3702 GN=KIN4C PE=2 SV=2)

HSP 1 Score: 180.6 bits (457), Expect = 3.7e-44
Identity = 162/535 (30.28%), Postives = 239/535 (44.67%), Query Frame = 0

Query: 30   YEKRVQELERQNEAFQKEIKDLKDRLEIASSSAANSAKKLREGYIQKLDVLGDQVIRDFF 89
            YEK+V +LE++  A Q+EI+ L+  L    S   + A+KL+E Y+QKL+ L         
Sbjct: 587  YEKKVYDLEQEKRALQREIEGLRHNLASIPSGPGDGAQKLKEEYVQKLNTLET------- 646

Query: 90   WSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQKVVQ 149
                             QV+ LKKK DAQ+QL  ++QK + A  +   EI  +K+QK VQ
Sbjct: 647  -----------------QVSVLKKKQDAQAQLMRQKQKSDDAAIKLQDEIHRIKSQK-VQ 706

Query: 150  LQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEEASE 209
            LQ K+K ES QFR  K S EKE++QLK++ RRNEY+M+ L+  NQ+ K+VLQRKTEEAS+
Sbjct: 707  LQQKIKQESEQFRAWKASREKEVMQLKKEGRRNEYEMHKLMALNQKQKLVLQRKTEEASQ 766

Query: 210  VTKRIRNLLESRRISAQRKAGAKHGINS---TQYVENEFEVTARLHELCSQYEHEIEEK- 269
            VTKR++ LL++R+ S++      +G  +    Q +E+E EVT R+HE+ S+YE + EE+ 
Sbjct: 767  VTKRLKELLDNRKASSRETLSGANGPGTQALMQAIEHEIEVTVRVHEVRSEYERQTEERA 826

Query: 270  --DKEIARLKEEAEAL-------------------------------------------- 329
               KE+ARL+EE E L                                            
Sbjct: 827  RMAKEVARLREENELLKNAKISVHGDTMSPGARNSRIFALENMLATSSSTLVSMASQLSE 886

Query: 330  --------------------------------MQETSGYLSQKTDVNILENNQDVNELKE 389
                                            +  T+  L++  + +  E +  + +LKE
Sbjct: 887  AEERERVFGGRGRWNQVRTLGDAKSIMNYLFNLASTARCLARDKEADCREKDVLIRDLKE 946

Query: 390  QMVVLSGLVNQMRIQKFKHTHR---------------DVSKDNSGTTSASVGSNNLFEQF 413
            ++V  S  V  M IQK    H+               ++  ++S     +  S  + E  
Sbjct: 947  KIVKFSSYVRYMEIQKADLVHQVKAQTSAMKKLSADENLKNEHSMKKQETRNSTIVLEDM 1006

BLAST of MS001149 vs. ExPASy Swiss-Prot
Match: B9F2Y7 (Kinesin-like protein KIN-4C OS=Oryza sativa subsp. japonica OX=39947 GN=KIN4C PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 5.0e-41
Identity = 164/546 (30.04%), Postives = 238/546 (43.59%), Query Frame = 0

Query: 30   YEKRVQELERQNEAFQKEIKDLKDRLEIASSSAANSAKKLREGYIQKLDVLGDQVIRDFF 89
            YEK++ E+E++ +A QKEI++L+  L   +SS   SA+KL+E Y+QKL+ L         
Sbjct: 603  YEKKLNEMEQEKKALQKEIEELRHALTNITSSTDESAQKLKENYLQKLNTLES------- 662

Query: 90   WSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQKVVQ 149
                             QV+ELKKK +AQ QL  ++Q+ + A K+   +I  +K+QK VQ
Sbjct: 663  -----------------QVSELKKKQEAQQQLIRQKQRSDEAAKRLQEDIHRIKSQK-VQ 722

Query: 150  LQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEEASE 209
            LQ K+K ES QFR  K + EKE+LQLK++ RRNEY+M+ L+  NQR KMVLQRKTEEA+ 
Sbjct: 723  LQQKIKQESEQFRSWKAAREKEVLQLKKEGRRNEYEMHKLLALNQRQKMVLQRKTEEAAM 782

Query: 210  VTKRIRNLLESRRISAQRKAGAK-HGINS-TQYVENEFEVTARLHELCSQYEHEIEEK-- 269
             TKR++  LE+++ +      A   GI +  + +++E EVT R +EL S YE +++E+  
Sbjct: 783  ATKRLKESLEAKKSTRDTYGSASGSGIQALMRAIDDELEVTVRAYELRSHYERQMQERAA 842

Query: 270  -DKEIARLKE------------------------------------EAEA---------- 329
              KEIA+LKE                                    EAE           
Sbjct: 843  ISKEIAKLKECPQAMSPSARSSRISALENMLSSSSSAMVSMASQLSEAEERERAFNGKGR 902

Query: 330  ----------------LMQETSGYLSQKTDVNIL--ENNQDVNELKEQMVVLSGLVNQMR 389
                            L Q  S    Q+ D  ++  E    + +LKE++V L+G + Q+ 
Sbjct: 903  WNHVRSLPDAKNTMNYLFQLASSSRCQQLDKEVMCKEKEHLICDLKEKVVALNGRIRQLE 962

Query: 390  IQ-------------KFKHTHRDVSKDNSGTTSASVG-----------------SNNLF- 419
             Q                     V     GT  +  G                 S N F 
Sbjct: 963  TQVKDLNNQNMLLFTAISEAKNPVGTSRKGTVGSEDGQHYAMRKSIRASHSLHYSKNSFL 1022

BLAST of MS001149 vs. ExPASy Swiss-Prot
Match: Q6YUL8 (Kinesin-like protein KIN-4A OS=Oryza sativa subsp. japonica OX=39947 GN=KIN4A PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.9e-25
Identity = 102/288 (35.42%), Postives = 160/288 (55.56%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRL--EIA 60
           + + +RQ  Q  E+++      +    + + K++ ELE +  A Q+E    +DRL  E+ 
Sbjct: 507 LNELNRQLEQK-ESEMKMYGSDTVALKQHFGKKLLELEEEKRAVQQE----RDRLLAEVE 566

Query: 61  SSSAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQ 120
           S +A     KLR+  +QKL  L  Q++                        +LKKK + Q
Sbjct: 567 SLNADGQTHKLRDAQLQKLKTLEAQIL------------------------DLKKKQENQ 626

Query: 121 SQLSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRD 180
            QL   +QK + A K+   EI S+KAQK VQLQ K+K E+ QFR  K + EKE+LQL+++
Sbjct: 627 VQLLKEKQKSDEAAKKLQEEIHSIKAQK-VQLQHKIKQEAEQFRQWKATREKELLQLRKE 686

Query: 181 NRRNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKA---GAKHGI 240
            RRNEY+ + L   NQR K+VLQRKTEEA+  TKR++ LLE+R+ S +  +   G   G 
Sbjct: 687 GRRNEYERHKLQALNQRQKLVLQRKTEEAAMATKRLKELLEARKSSGRDNSGMNGTSPGS 746

Query: 241 NSTQ-----YVENEFEVTARLHELCSQYEHEIEEK---DKEIARLKEE 276
           + T+     ++E + EV   +HE+ ++YE + + +    +E+A LK+E
Sbjct: 747 HMTEKSLQKWLEQDLEVMVHVHEVRNEYEKQSQLRAALGEELAILKQE 764

BLAST of MS001149 vs. ExPASy Swiss-Prot
Match: A0A068FIK2 (Kinesin-like protein KIN-4A OS=Gossypium hirsutum OX=3635 GN=KIN4A PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 5.0e-25
Identity = 95/261 (36.40%), Postives = 144/261 (55.17%), Query Frame = 0

Query: 30  YEKRVQELERQNEAFQKEIKDLKDRLEIASSSAANSAKKLREGYIQKLDVLGDQVIRDFF 89
           + K++QELE +  A Q+E   L   +E  S+ +   A K+ + + QKL  L  Q++    
Sbjct: 530 FGKKIQELEEEKRAVQQERDRLLAEIENLSAGSEGQALKVHDIHAQKLKSLEAQIM---- 589

Query: 90  WSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQKVVQ 149
                               +LKKK + Q QL  ++QK + A K+   EI  +KAQK VQ
Sbjct: 590 --------------------DLKKKQENQVQLLKKKQKSDEAAKRLQDEIQYIKAQK-VQ 649

Query: 150 LQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEEASE 209
           LQ ++K E+ QFR  K S EKE+LQL+++ RRNEY+ + L   NQR K+VLQRKTEEA+ 
Sbjct: 650 LQHRIKQEAEQFRQWKASREKELLQLRKEGRRNEYERHKLQALNQRQKLVLQRKTEEAAM 709

Query: 210 VTKRIRNLLESRRISAQRKAGAKHG------INS---TQYVENEFEVTARLHELCSQYEH 269
            TKR++ LLE+R+ +A+      +G      IN     +++++E EV   +HE+  +YE 
Sbjct: 710 ATKRLKELLEARKSAARDNLAIANGNGTNGKINEKGLQRWLDHELEVMVNVHEVRFEYEK 761

Query: 270 EIEEKDKEIARLKEEAEALMQ 282
           + + +    A L EE   L Q
Sbjct: 770 QSQVR----AALAEELAVLKQ 761

BLAST of MS001149 vs. ExPASy Swiss-Prot
Match: Q8GS71 (Kinesin-like protein KIN-4A OS=Arabidopsis thaliana OX=3702 GN=KIN4A PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.3e-24
Identity = 92/263 (34.98%), Postives = 147/263 (55.89%), Query Frame = 0

Query: 30  YEKRVQELERQNEAFQKEIKDLKDRL--EIASSSAANSAKKLREGYIQKLDVLGDQVIRD 89
           + K++ E+E +  + Q+E    ++RL  EI + ++   A+KL++ + Q L  L  Q++  
Sbjct: 540 FGKKIAEVEDEKRSVQEE----RNRLLAEIENLASDGQAQKLQDVHAQNLKALEAQIL-- 599

Query: 90  FFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQKV 149
                                 +LKKK ++Q QL  ++QK + A ++   EI S+KAQK 
Sbjct: 600 ----------------------DLKKKQESQVQLLKQKQKSDDAARRLQDEIQSIKAQK- 659

Query: 150 VQLQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEEA 209
           VQLQ +MK E+ QFR  K S EKE+LQL+++ R++EY+ + L   NQR KMVLQRKTEEA
Sbjct: 660 VQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQKMVLQRKTEEA 719

Query: 210 SEVTKRIRNLLESRRISAQRKAGAKHGINST---------QYVENEFEVTARLHELCSQY 269
           +  TKR++ LLE+R+ S +  +   +G  +          +++++E EV   +HE+    
Sbjct: 720 AMATKRLKELLEARKSSPREHSAGTNGFGTNGQTNEKSLQRWLDHELEVMVNVHEV---- 769

Query: 270 EHEIEEKDKEIARLKEEAEALMQ 282
            HE E++    A L EE   L Q
Sbjct: 780 RHEYEKQSHVRAALAEELAVLRQ 769

BLAST of MS001149 vs. ExPASy TrEMBL
Match: A0A6J1BSZ2 (kinesin-like protein KIN-4C OS=Momordica charantia OX=3673 GN=LOC111004566 PE=4 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 5.7e-213
Identity = 410/437 (93.82%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKASRQSRQTGEADVSSSPVTSATDNKDYE RVQELERQNEAFQKEIKDLKDRLEIASS
Sbjct: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYENRVQELERQNEAFQKEIKDLKDRLEIASS 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLDVLGD                        QVTELKKKLDAQSQ
Sbjct: 61  SAANSAKKLREGYIQKLDVLGD------------------------QVTELKKKLDAQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LSIRRQKGEGATKQTDLEITSLKAQK VQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR
Sbjct: 121 LSIRRQKGEGATKQTDLEITSLKAQK-VQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHGINSTQY 240
           RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHGINSTQY
Sbjct: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHGINSTQY 240

Query: 241 VENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENNQ 300
           VENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENNQ
Sbjct: 241 VENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENNQ 300

Query: 301 DVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEHG 360
           DVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKD+SGTTSASVGSNNLFEQFDTAGEEHG
Sbjct: 301 DVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDDSGTTSASVGSNNLFEQFDTAGEEHG 360

Query: 361 QGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGETI 420
           QGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGETI
Sbjct: 361 QGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGETI 412

Query: 421 HEFGPPMKPLSDIKNVL 438
           HEFGPPMKPLSDIKNVL
Sbjct: 421 HEFGPPMKPLSDIKNVL 412

BLAST of MS001149 vs. ExPASy TrEMBL
Match: A0A6J1KJV2 (kinesin-like protein KIN-4C OS=Cucurbita maxima OX=3661 GN=LOC111495924 PE=4 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 5.8e-165
Identity = 329/438 (75.11%), Postives = 364/438 (83.11%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKASR ++Q GEAD S S VTSATD++DYEKR+QELER+N+AFQKEIKDLKDRLEIASS
Sbjct: 1   MRKASRPTKQKGEADASPSSVTSATDSRDYEKRIQELERENKAFQKEIKDLKDRLEIASS 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLD+L D                        QVTELKKKLD QSQ
Sbjct: 61  SAANSAKKLREGYIQKLDILED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS RR KG+GATKQTD EI SLKAQK V LQCKMKLESVQFRLCKGSLEKE+LQLKR+NR
Sbjct: 121 LSTRRPKGDGATKQTDFEIMSLKAQK-VHLQCKMKLESVQFRLCKGSLEKEVLQLKRENR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAK-HGINSTQ 240
           RNE++MN L TSNQRLKMVLQRKTEEASE+TK+IRNLLESRR SAQR+AGA+   + S+Q
Sbjct: 181 RNEHEMNKLRTSNQRLKMVLQRKTEEASEITKKIRNLLESRRTSAQRRAGARQENVTSSQ 240

Query: 241 YVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENN 300
            VENEFEVTA LHELCSQYEH+IEEKDKEIA+L+EE +AL QE +GY S +TD N+L NN
Sbjct: 241 SVENEFEVTASLHELCSQYEHQIEEKDKEIAKLQEEVDALKQEETGYQSLETD-NVLGNN 300

Query: 301 QDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEH 360
           QD  ELKEQMV+LSGLVNQMRIQKF HT +  SKD+SG +SAS GSNNLFEQF  AGEEH
Sbjct: 301 QDAAELKEQMVILSGLVNQMRIQKFNHTQKGESKDDSGPSSASNGSNNLFEQFAIAGEEH 360

Query: 361 GQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGET 420
            +GF + TKR VKAECCSCSKKSLCKT KCKCRSTGSSCG+SCGCA GKCSNR EKP E 
Sbjct: 361 WRGFNVATKRAVKAECCSCSKKSLCKTTKCKCRSTGSSCGSSCGCAHGKCSNRDEKPSEM 412

Query: 421 IHEFGPPMKPLSDIKNVL 438
           + EFGPPM PLSDIKN+L
Sbjct: 421 VDEFGPPMNPLSDIKNLL 412

BLAST of MS001149 vs. ExPASy TrEMBL
Match: A0A6J1KAP1 (kinesin-like protein KIN-4C isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492146 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.4e-163
Identity = 335/437 (76.66%), Postives = 365/437 (83.52%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKA+RQS++TGEADVSSS VTSATD+KDYEKR++ELE +NEAFQKEIKDLK+RLE AS 
Sbjct: 1   MRKATRQSKKTGEADVSSSSVTSATDSKDYEKRIRELESENEAFQKEIKDLKNRLENASP 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLDVL D                        QVTELKKKLD QSQ
Sbjct: 61  SAANSAKKLREGYIQKLDVLED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS R +KG+G +KQTDLEI SLKAQK VQLQCKMKLESVQFRL KGSLEKE+LQLKR+ R
Sbjct: 121 LSTRWKKGDGVSKQTDLEIMSLKAQK-VQLQCKMKLESVQFRLYKGSLEKEVLQLKREKR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHG-INSTQ 240
           RNE++MN L+TSNQRLKMVLQRKTEEASEVTKRIRNLLESRR SAQR+AGAKHG I STQ
Sbjct: 181 RNEHEMNKLLTSNQRLKMVLQRKTEEASEVTKRIRNLLESRRTSAQRRAGAKHGNITSTQ 240

Query: 241 YVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENN 300
           YVENEFEVT RLHELCSQYEH IEEKDKEIA+L+EEA+AL QE SGY SQ TD NILENN
Sbjct: 241 YVENEFEVTVRLHELCSQYEHLIEEKDKEIAKLQEEADALKQEKSGYPSQDTDDNILENN 300

Query: 301 QDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEH 360
           QD+NELKEQMV+LSGL NQMRIQKF HT +D SKD S  TS S GSNNL E+FD+AGEEH
Sbjct: 301 QDMNELKEQMVMLSGLFNQMRIQKFNHTPKDASKDVSDQTSPSFGSNNLCERFDSAGEEH 360

Query: 361 GQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGET 420
            Q  + V K TVK ECCSCSKKSLCKT KC+CRSTG SCG SCGCA+GKCSNRYEKPGE 
Sbjct: 361 RQ--DNVKKITVKEECCSCSKKSLCKTTKCQCRSTGRSCGTSCGCALGKCSNRYEKPGEM 408

Query: 421 IHEFGPPMKPLSDIKNV 437
           I+  G P+KPLSDIKNV
Sbjct: 421 IN--GQPLKPLSDIKNV 408

BLAST of MS001149 vs. ExPASy TrEMBL
Match: A0A6J1GHQ1 (kinesin-like protein KIN-4C OS=Cucurbita moschata OX=3662 GN=LOC111454037 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 7.0e-163
Identity = 329/438 (75.11%), Postives = 362/438 (82.65%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKASR ++Q GEAD SSS VTSATD+ DYEKR+QELER+N+AFQKEIKDLK RLEIASS
Sbjct: 1   MRKASRPTKQKGEADASSSSVTSATDSIDYEKRIQELERENKAFQKEIKDLKVRLEIASS 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLDVL D                        QVTELKKKLD QSQ
Sbjct: 61  SAANSAKKLREGYIQKLDVLED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS RR KG+GATKQTD EI SLKAQK VQLQCKMKLESVQFRLCKGSLEKE+LQLKR+NR
Sbjct: 121 LSTRRPKGDGATKQTDFEIMSLKAQK-VQLQCKMKLESVQFRLCKGSLEKEVLQLKRENR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAK-HGINSTQ 240
           RNE++MN L TSNQRLKMVLQRKTEEASE+TK++RNLLESRR SAQR+ GA+   + S+Q
Sbjct: 181 RNEHEMNKLRTSNQRLKMVLQRKTEEASEITKKMRNLLESRRTSAQRRTGARQENVTSSQ 240

Query: 241 YVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENN 300
            VENEFEVTA LHELCSQYEH+IEEKDKEIA+L+EEA+AL QE +GY S +TD N+L NN
Sbjct: 241 SVENEFEVTASLHELCSQYEHQIEEKDKEIAKLQEEADALKQEKTGYQSLETD-NVLGNN 300

Query: 301 QDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEH 360
           QDV ELKEQMV+LSGLVNQMRIQKF HT +  SKD+SG +SAS GS NLFEQF  AGEEH
Sbjct: 301 QDVAELKEQMVILSGLVNQMRIQKFNHTQKGESKDDSGPSSASNGSINLFEQFSIAGEEH 360

Query: 361 GQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGET 420
             GF + TKR VKAECCSCSKKSLCKT KCKCRSTGSSCG+SCGCA GKCSNR EKP E 
Sbjct: 361 RHGFNVATKRAVKAECCSCSKKSLCKTTKCKCRSTGSSCGSSCGCAHGKCSNRDEKPSEM 412

Query: 421 IHEFGPPMKPLSDIKNVL 438
           + EFGP M PLSDIKN+L
Sbjct: 421 VDEFGPQMNPLSDIKNLL 412

BLAST of MS001149 vs. ExPASy TrEMBL
Match: A0A6J1HBC2 (kinesin-like protein KIN-4C isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462456 PE=4 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.9e-161
Identity = 331/437 (75.74%), Postives = 362/437 (82.84%), Query Frame = 0

Query: 1   MRKASRQSRQTGEADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASS 60
           MRKA RQS++TGEADVSSS +TSATD+KDYEKR++ELE +NEAFQKEIKDLK+RLE AS 
Sbjct: 1   MRKAIRQSKKTGEADVSSSSITSATDSKDYEKRIRELESENEAFQKEIKDLKNRLENASP 60

Query: 61  SAANSAKKLREGYIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQ 120
           SAANSAKKLREGYIQKLDVL D                        QVTELKKKLD QSQ
Sbjct: 61  SAANSAKKLREGYIQKLDVLED------------------------QVTELKKKLDVQSQ 120

Query: 121 LSIRRQKGEGATKQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNR 180
           LS R +KG+  +KQTDLEI SLKAQK VQLQCKMKLESVQFRL KGSLEKE+LQLKR+ R
Sbjct: 121 LSTRWKKGDEVSKQTDLEIMSLKAQK-VQLQCKMKLESVQFRLYKGSLEKEVLQLKREKR 180

Query: 181 RNEYKMNNLITSNQRLKMVLQRKTEEASEVTKRIRNLLESRRISAQRKAGAKHG-INSTQ 240
           RNE++MN L+TSNQRLKMVLQRK EEASEVTKRIRNLLESRR SAQR+AGAKHG I STQ
Sbjct: 181 RNEHEMNKLLTSNQRLKMVLQRKAEEASEVTKRIRNLLESRRTSAQRRAGAKHGNITSTQ 240

Query: 241 YVENEFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQETSGYLSQKTDVNILENN 300
           YVENEFEVTARLHELCSQYEH IEEKDKEIA+L+EEA+AL QE SGY SQ TD N+LENN
Sbjct: 241 YVENEFEVTARLHELCSQYEHLIEEKDKEIAKLQEEADALKQEKSGYPSQDTDDNMLENN 300

Query: 301 QDVNELKEQMVVLSGLVNQMRIQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEH 360
           QD+NELKEQMV+LSGL NQMRIQK  HT +D SKD S  TS S GSNNL EQFD+AGEEH
Sbjct: 301 QDMNELKEQMVMLSGLFNQMRIQKLNHTPKDASKDVSDQTSPSFGSNNLCEQFDSAGEEH 360

Query: 361 GQGFEMVTKRTVKAECCSCSKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYEKPGET 420
            Q  + V K T+K ECCSCSKKSLCKT KC+CRSTG SCG SCGCA+GKCSNRYEKPGE 
Sbjct: 361 QQ--DNVKKITLKEECCSCSKKSLCKTTKCQCRSTGRSCGTSCGCALGKCSNRYEKPGEM 408

Query: 421 IHEFGPPMKPLSDIKNV 437
           I+  G P+KPLSDIKNV
Sbjct: 421 IN--GQPLKPLSDIKNV 408

BLAST of MS001149 vs. TAIR 10
Match: AT5G33300.1 (chromosome-associated kinesin-related )

HSP 1 Score: 182.6 bits (462), Expect = 7.0e-46
Identity = 149/433 (34.41%), Postives = 229/433 (52.89%), Query Frame = 0

Query: 28  KDYEKRVQELERQNEAFQKEIKDLKDRL-EIASSSAANSAKKLREGYIQKLDVLGDQVIR 87
           K YEK++ +LE  NEA + ++++L+ +L +++ SS+  + +  R+               
Sbjct: 20  KLYEKKILDLESVNEALKSDVEELRSKLADVSISSSVGTLQSSRK--------------- 79

Query: 88  DFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQK 147
                         +  K     E      ++S  S ++ K E + KQ D E+  LKAQK
Sbjct: 80  --------------FSQKSIANKEEGMSSRSKSMCSTKKYKTESSVKQFDGEVQKLKAQK 139

Query: 148 VVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEE 207
            V+L CK+KL+S+QFRL K SLEKE+LQLK++ R++E++ + L   N R K++LQ K  +
Sbjct: 140 -VKLHCKIKLDSMQFRLLKASLEKEVLQLKKELRKSEFEKHVLSALNNRQKLILQLKNTQ 199

Query: 208 ASEVTKRIRNLLESRRISAQRKAGAKHGINS-TQYVENEFEVTARLHELCSQYEHEIEEK 267
           A    KR++ LL+S++IS+ +K G   G +S  Q   NE  +  +L+++ S YE +++E 
Sbjct: 200 ALTALKRLKMLLQSKKISSNKKKGPSKGTSSEIQESSNESGLLMKLNKIHSDYERQMKEM 259

Query: 268 DKEIARLKEEAEALMQETSGYLSQ-----KTDVNILENNQDVNELKEQMVVLSGLVNQMR 327
            +EI R   EA  L  E  G  S         +N    + ++ ELKE+   LS LV+QM 
Sbjct: 260 AEEIKRFSLEAGLLKAEFEGEQSSCSASCDNQINHTPMDSELKELKEEFNKLSTLVSQME 319

Query: 328 IQKFKHTHRDVSKDNSGTTSASVGSNNLFEQFDTAGEEHGQGFEMVTKR-TVKAE-CCSC 387
           + K + +  D  K  S     S+ S N+ ++ ++   +     E + K+   KAE CCSC
Sbjct: 320 MTKSQFSETD--KVQSEPVERSITSKNIDDESNSEPFQPETSEETLCKKEQSKAEVCCSC 379

Query: 388 SKKSLCKTMKCKCRSTGSSCGASCGCAIGKCSNRYE-----KPGETI----------HEF 437
           +KKSLCKT  CKC++ GS CG SCGC   KCSNR E     KP E I           + 
Sbjct: 380 TKKSLCKTKSCKCKANGSGCGDSCGCLASKCSNRDESAKPDKPMEPIDVKKPAGISHDDK 420

BLAST of MS001149 vs. TAIR 10
Match: AT5G60930.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 2.6e-45
Identity = 162/535 (30.28%), Postives = 239/535 (44.67%), Query Frame = 0

Query: 30   YEKRVQELERQNEAFQKEIKDLKDRLEIASSSAANSAKKLREGYIQKLDVLGDQVIRDFF 89
            YEK+V +LE++  A Q+EI+ L+  L    S   + A+KL+E Y+QKL+ L         
Sbjct: 587  YEKKVYDLEQEKRALQREIEGLRHNLASIPSGPGDGAQKLKEEYVQKLNTLET------- 646

Query: 90   WSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQKVVQ 149
                             QV+ LKKK DAQ+QL  ++QK + A  +   EI  +K+QK VQ
Sbjct: 647  -----------------QVSVLKKKQDAQAQLMRQKQKSDDAAIKLQDEIHRIKSQK-VQ 706

Query: 150  LQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEEASE 209
            LQ K+K ES QFR  K S EKE++QLK++ RRNEY+M+ L+  NQ+ K+VLQRKTEEAS+
Sbjct: 707  LQQKIKQESEQFRAWKASREKEVMQLKKEGRRNEYEMHKLMALNQKQKLVLQRKTEEASQ 766

Query: 210  VTKRIRNLLESRRISAQRKAGAKHGINS---TQYVENEFEVTARLHELCSQYEHEIEEK- 269
            VTKR++ LL++R+ S++      +G  +    Q +E+E EVT R+HE+ S+YE + EE+ 
Sbjct: 767  VTKRLKELLDNRKASSRETLSGANGPGTQALMQAIEHEIEVTVRVHEVRSEYERQTEERA 826

Query: 270  --DKEIARLKEEAEAL-------------------------------------------- 329
               KE+ARL+EE E L                                            
Sbjct: 827  RMAKEVARLREENELLKNAKISVHGDTMSPGARNSRIFALENMLATSSSTLVSMASQLSE 886

Query: 330  --------------------------------MQETSGYLSQKTDVNILENNQDVNELKE 389
                                            +  T+  L++  + +  E +  + +LKE
Sbjct: 887  AEERERVFGGRGRWNQVRTLGDAKSIMNYLFNLASTARCLARDKEADCREKDVLIRDLKE 946

Query: 390  QMVVLSGLVNQMRIQKFKHTHR---------------DVSKDNSGTTSASVGSNNLFEQF 413
            ++V  S  V  M IQK    H+               ++  ++S     +  S  + E  
Sbjct: 947  KIVKFSSYVRYMEIQKADLVHQVKAQTSAMKKLSADENLKNEHSMKKQETRNSTIVLEDM 1006

BLAST of MS001149 vs. TAIR 10
Match: AT5G47820.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 2.3e-25
Identity = 92/263 (34.98%), Postives = 147/263 (55.89%), Query Frame = 0

Query: 30  YEKRVQELERQNEAFQKEIKDLKDRL--EIASSSAANSAKKLREGYIQKLDVLGDQVIRD 89
           + K++ E+E +  + Q+E    ++RL  EI + ++   A+KL++ + Q L  L  Q++  
Sbjct: 540 FGKKIAEVEDEKRSVQEE----RNRLLAEIENLASDGQAQKLQDVHAQNLKALEAQIL-- 599

Query: 90  FFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQKV 149
                                 +LKKK ++Q QL  ++QK + A ++   EI S+KAQK 
Sbjct: 600 ----------------------DLKKKQESQVQLLKQKQKSDDAARRLQDEIQSIKAQK- 659

Query: 150 VQLQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEEA 209
           VQLQ +MK E+ QFR  K S EKE+LQL+++ R++EY+ + L   NQR KMVLQRKTEEA
Sbjct: 660 VQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQKMVLQRKTEEA 719

Query: 210 SEVTKRIRNLLESRRISAQRKAGAKHGINST---------QYVENEFEVTARLHELCSQY 269
           +  TKR++ LLE+R+ S +  +   +G  +          +++++E EV   +HE+    
Sbjct: 720 AMATKRLKELLEARKSSPREHSAGTNGFGTNGQTNEKSLQRWLDHELEVMVNVHEV---- 769

Query: 270 EHEIEEKDKEIARLKEEAEALMQ 282
            HE E++    A L EE   L Q
Sbjct: 780 RHEYEKQSHVRAALAEELAVLRQ 769

BLAST of MS001149 vs. TAIR 10
Match: AT5G47820.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 2.3e-25
Identity = 92/263 (34.98%), Postives = 147/263 (55.89%), Query Frame = 0

Query: 30  YEKRVQELERQNEAFQKEIKDLKDRL--EIASSSAANSAKKLREGYIQKLDVLGDQVIRD 89
           + K++ E+E +  + Q+E    ++RL  EI + ++   A+KL++ + Q L  L  Q++  
Sbjct: 540 FGKKIAEVEDEKRSVQEE----RNRLLAEIENLASDGQAQKLQDVHAQNLKALEAQIL-- 599

Query: 90  FFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGATKQTDLEITSLKAQKV 149
                                 +LKKK ++Q QL  ++QK + A ++   EI S+KAQK 
Sbjct: 600 ----------------------DLKKKQESQVQLLKQKQKSDDAARRLQDEIQSIKAQK- 659

Query: 150 VQLQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITSNQRLKMVLQRKTEEA 209
           VQLQ +MK E+ QFR  K S EKE+LQL+++ R++EY+ + L   NQR KMVLQRKTEEA
Sbjct: 660 VQLQHRMKQEAEQFRQWKASREKELLQLRKEGRKSEYERHKLQALNQRQKMVLQRKTEEA 719

Query: 210 SEVTKRIRNLLESRRISAQRKAGAKHGINST---------QYVENEFEVTARLHELCSQY 269
           +  TKR++ LLE+R+ S +  +   +G  +          +++++E EV   +HE+    
Sbjct: 720 AMATKRLKELLEARKSSPREHSAGTNGFGTNGQTNEKSLQRWLDHELEVMVNVHEV---- 769

Query: 270 EHEIEEKDKEIARLKEEAEALMQ 282
            HE E++    A L EE   L Q
Sbjct: 780 RHEYEKQSHVRAALAEELAVLRQ 769

BLAST of MS001149 vs. TAIR 10
Match: AT3G50240.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 113.6 bits (283), Expect = 4.0e-25
Identity = 95/279 (34.05%), Postives = 150/279 (53.76%), Query Frame = 0

Query: 13  EADVSSSPVTSATDNKDYEKRVQELERQNEAFQKEIKDLKDRLEIASSSAANSAKKLREG 72
           E+++    + + T  + +EK++ ELE++    Q E   L   +E  ++S+   A+  R+ 
Sbjct: 526 ESEMRVCGIGTETIRQHFEKKMMELEKEKRTVQDERDMLLAEVEELAASSDRQAQVARDN 585

Query: 73  YIQKLDVLGDQVIRDFFWSHLDKHVINCYDVKFFQVTELKKKLDAQSQLSIRRQKGEGAT 132
           +  KL  L  Q++                         LKKK + Q ++  ++QK E A 
Sbjct: 586 HAHKLKALETQIL------------------------NLKKKQENQVEVLKQKQKSEDAA 645

Query: 133 KQTDLEITSLKAQKVVQLQCKMKLESVQFRLCKGSLEKEILQLKRDNRRNEYKMNNLITS 192
           K+   EI  +KAQK VQLQ KMK E+ QFR  K S EKE+LQLK++ R+ E++   L   
Sbjct: 646 KRLKTEIQCIKAQK-VQLQQKMKQEAEQFRQWKASQEKELLQLKKEGRKTEHERLKLEAL 705

Query: 193 NQRLKMVLQRKTEEASEVTKRIRNLLESRRIS---------AQRKAGAKHGINSTQYVEN 252
           N+R KMVLQRKTEEA+  TKR++ LLE+R+ S          Q  +   +  +  ++++N
Sbjct: 706 NRRQKMVLQRKTEEAAMATKRLKELLEARKSSPHDISVIANGQPPSRQTNEKSLRKWLDN 765

Query: 253 EFEVTARLHELCSQYEHEIEEKDKEIARLKEEAEALMQE 283
           E EV A++H++  QYE +I+ +    A L  E  +L QE
Sbjct: 766 ELEVMAKVHQVRFQYEKQIQVR----AALAVELTSLRQE 775