MS001127 (gene) Bitter gourd (TR) v1

Overview


Name	MS001127
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Protein kinase domain-containing protein
Location	scaffold36: 1638304 .. 1638873 (-)
RNA-Seq Expression	MS001127
Synteny	MS001127

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCAGAAAAAGAAATAGGCCTCTCAACATCAGGGAATTTCCCTACTCCGATCTCGTCTCCTCCACCGACGGCTTCTCCGCCGACTCTTTCCTGGGCAAAGGCAGCCACGGCAGCGTCTACAAGGCGGTTCTCGACTGCGGCAAACTCCTAGCCGCCGTCAAGCGGACTAAAATTTCACACCCATCTTCCCATTTTCACCACGCCCATGATTACCGATGTGACATTAATTCCTGCCAATTCGCCACTAATACTCCCGCCGAAAACGAAATCGAGATTCTCTCGCGCCTCTACAACCGTCGGATCGTCAATCTCATCGGCTTTTGCGCCGATGCCAAGGACGAGAAATTGATTGTCGTCGAGTTCATGCCCAATGGCTCTCTCTACGATCTCCTTCACTCGCGGTCCAGGCCGCCCACTTGGAACCGGCGGGTCCGGTTCGCGCTGCAGGTTGCCAAGGCGGTTCGAGCCCTTCACGCGGCGAACCCGCCGGTTATTCACCGGGATATTAAGTCGTCCAACGTCTTGATCGACGGTAACTTCCACGCGCGGCTTGGCGACTTCGGGCTGGCC

mRNA sequence

CCCAGAAAAAGAAATAGGCCTCTCAACATCAGGGAATTTCCCTACTCCGATCTCGTCTCCTCCACCGACGGCTTCTCCGCCGACTCTTTCCTGGGCAAAGGCAGCCACGGCAGCGTCTACAAGGCGGTTCTCGACTGCGGCAAACTCCTAGCCGCCGTCAAGCGGACTAAAATTTCACACCCATCTTCCCATTTTCACCACGCCCATGATTACCGATGTGACATTAATTCCTGCCAATTCGCCACTAATACTCCCGCCGAAAACGAAATCGAGATTCTCTCGCGCCTCTACAACCGTCGGATCGTCAATCTCATCGGCTTTTGCGCCGATGCCAAGGACGAGAAATTGATTGTCGTCGAGTTCATGCCCAATGGCTCTCTCTACGATCTCCTTCACTCGCGGTCCAGGCCGCCCACTTGGAACCGGCGGGTCCGGTTCGCGCTGCAGGTTGCCAAGGCGGTTCGAGCCCTTCACGCGGCGAACCCGCCGGTTATTCACCGGGATATTAAGTCGTCCAACGTCTTGATCGACGGTAACTTCCACGCGCGGCTTGGCGACTTCGGGCTGGCC

Coding sequence (CDS)

CCCAGAAAAAGAAATAGGCCTCTCAACATCAGGGAATTTCCCTACTCCGATCTCGTCTCCTCCACCGACGGCTTCTCCGCCGACTCTTTCCTGGGCAAAGGCAGCCACGGCAGCGTCTACAAGGCGGTTCTCGACTGCGGCAAACTCCTAGCCGCCGTCAAGCGGACTAAAATTTCACACCCATCTTCCCATTTTCACCACGCCCATGATTACCGATGTGACATTAATTCCTGCCAATTCGCCACTAATACTCCCGCCGAAAACGAAATCGAGATTCTCTCGCGCCTCTACAACCGTCGGATCGTCAATCTCATCGGCTTTTGCGCCGATGCCAAGGACGAGAAATTGATTGTCGTCGAGTTCATGCCCAATGGCTCTCTCTACGATCTCCTTCACTCGCGGTCCAGGCCGCCCACTTGGAACCGGCGGGTCCGGTTCGCGCTGCAGGTTGCCAAGGCGGTTCGAGCCCTTCACGCGGCGAACCCGCCGGTTATTCACCGGGATATTAAGTCGTCCAACGTCTTGATCGACGGTAACTTCCACGCGCGGCTTGGCGACTTCGGGCTGGCC

Protein sequence

PRKRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPSSHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFMPNGSLYDLLHSRSRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFHARLGDFGLA

Homology

[Close]

BLAST of MS001127 vs. NCBI nr
Match: XP_023536759.1 (serine/threonine-protein kinase-like protein At3g51990 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 299.7 bits (766), Expect = 1.8e-77
Identity = 155/189 (82.01%), Postives = 168/189 (88.89%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK ++PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDAGKLVAAVKRTKSTNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S+FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFCAD+  EKLIVVEFM
Sbjct: 83  SNFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCADS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRVRFALQVAKAVR LHA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVRFALQVAKAVRDLHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. NCBI nr
Match: XP_023002316.1 (serine/threonine-protein kinase-like protein At1g28390 [Cucurbita maxima])

HSP 1 Score: 297.4 bits (760), Expect = 9.0e-77
Identity = 154/189 (81.48%), Postives = 166/189 (87.83%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK  +PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDAGKLVAAVKRTKSPNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S+FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFC D+  EKLIVVEFM
Sbjct: 83  SNFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCVDS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRVRFALQVAKAVR LHA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVRFALQVAKAVRDLHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. NCBI nr
Match: KAG6585357.1 (Serine/threonine-protein kinase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 297.0 bits (759), Expect = 1.2e-76
Identity = 154/189 (81.48%), Postives = 167/189 (88.36%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK  +PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDGGKLVAAVKRTKSPNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S+FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFCAD+  EKLIVVEFM
Sbjct: 83  SNFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCADS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRVRFALQVAKAVR +HA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVRFALQVAKAVRDIHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. NCBI nr
Match: KAG7020269.1 (Serine/threonine-protein kinase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 296.2 bits (757), Expect = 2.0e-76
Identity = 154/189 (81.48%), Postives = 166/189 (87.83%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK  +PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDAGKLVAAVKRTKSPNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S+FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFCAD+  EKLIVVEFM
Sbjct: 83  SNFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCADS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRV FALQVAKAVR LHA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVLFALQVAKAVRDLHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. NCBI nr
Match: XP_022951039.1 (serine/threonine-protein kinase-like protein At3g51990 isoform X2 [Cucurbita moschata])

HSP 1 Score: 295.0 bits (754), Expect = 4.5e-76
Identity = 154/189 (81.48%), Postives = 165/189 (87.30%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK  +PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDAGKLVAAVKRTKSPNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFCAD+  EKLIVVEFM
Sbjct: 83  STFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCADS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRV FALQVAKAVR LHA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVLFALQVAKAVRDLHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. ExPASy Swiss-Prot
Match: Q9SGN7 (Serine/threonine-protein kinase-like protein At1g28390 OS=Arabidopsis thaliana OX=3702 GN=At1g28390 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 7.0e-48
Identity = 108/195 (55.38%), Postives = 136/195 (69.74%), Query Frame = 0


Query: 1   PRKRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISH 60
           P KR+ P  +R F Y +L  +T+GFSA++FLGKGSHG VYKAVLD GKLLAAVKRT I+ 
Sbjct: 29  PEKRHPP-KLRVFNYDELAVATNGFSANNFLGKGSHGRVYKAVLDDGKLLAAVKRTTITT 88

Query: 61  PSSHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCAD-AKDEKLIVV 120
              + ++                +  +NEIEILSR+ +R +VNLIG+C D  +  KL+VV
Sbjct: 89  TVGNNNN--------------NVSQVDNEIEILSRVRHRWMVNLIGYCVDHRRKTKLLVV 148

Query: 121 EFMPNGSLYDLLHSR----SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVL 180
           E+MPNG+L+D LHSR    SR  +WNRR++ ALQ+A AV ALH A   VIHRDIKS NVL
Sbjct: 149 EYMPNGTLHDQLHSRSSLDSRLSSWNRRIKHALQIAIAVHALHTAETQVIHRDIKSCNVL 208

Query: 181 IDGNFHARLGDFGLA 191
           IDG+ +ARL DFGLA
Sbjct: 209 IDGDGNARLADFGLA 208

[Close]

BLAST of MS001127 vs. ExPASy Swiss-Prot
Match: Q9SV05 (Serine/threonine-protein kinase-like protein At3g51990 OS=Arabidopsis thaliana OX=3702 GN=At3g51990 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.0e-38
Identity = 92/191 (48.17%), Postives = 118/191 (61.78%), Query Frame = 0


Query: 5   NRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPSSH 64
           +RP  +R F + DL S+T GF  ++ LG+GSHGSVYKAV+  G    AVKR   S   S 
Sbjct: 40  DRP-RLRRFLHRDLESATGGFDINNLLGRGSHGSVYKAVI--GSRHIAVKRPSKSREISR 99

Query: 65  FHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFMPN 124
             H                    NE EILSR+ + R VNL+GF AD   E L+VVEFM N
Sbjct: 100 EFH--------------------NEFEILSRIRSPRFVNLLGFSADNSKEPLLVVEFMGN 159

Query: 125 GSLYDLLHS-----RSRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGN 184
           GSLYD++HS          +W++R++ ALQ+AKAV  LH+   P+IHRDIKS+NVL+D N
Sbjct: 160 GSLYDVIHSDTVLNSGAISSWSKRIKIALQIAKAVHLLHSQETPIIHRDIKSANVLMDKN 207

Query: 185 FHARLGDFGLA 191
            +A+LGDFGLA
Sbjct: 220 LNAKLGDFGLA 207

[Close]

BLAST of MS001127 vs. ExPASy Swiss-Prot
Match: O24585 (Putative receptor protein kinase CRINKLY4 OS=Zea mays OX=4577 GN=CR4 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.1e-31
Identity = 78/188 (41.49%), Postives = 110/188 (58.51%), Query Frame = 0


Query: 11  REFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLA---AVKRTKISHPSSHFHH 70
           +EF Y +L  +T GFS DS +GKGS   V+K +L  G ++A   A+K + +   S  FH 
Sbjct: 491 QEFSYEELEQATGGFSEDSQVGKGSFSCVFKGILRDGTVVAVKRAIKASDVKKSSKEFH- 550

Query: 71  AHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFMPNGSL 130
                               NE+++LSRL +  ++NL+G+C D   E+L+V EFM +GSL
Sbjct: 551 --------------------NELDLLSRLNHAHLLNLLGYCEDG-SERLLVYEFMAHGSL 610

Query: 131 YDLLHSR----SRPPTWNRRVRFALQVAKAVRALHA-ANPPVIHRDIKSSNVLIDGNFHA 190
           Y  LH +     +   W RRV  A+Q A+ +  LH  A PPVIHRDIKSSN+LID + +A
Sbjct: 611 YQHLHGKDPNLKKRLNWARRVTIAVQAARGIEYLHGYACPPVIHRDIKSSNILIDEDHNA 656

[Close]

BLAST of MS001127 vs. ExPASy Swiss-Prot
Match: Q75J39 (Serine/threonine-protein kinase-like protein CR4 OS=Oryza sativa subsp. japonica OX=39947 GN=CR4 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.0e-30
Identity = 77/188 (40.96%), Postives = 109/188 (57.98%), Query Frame = 0


Query: 11  REFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLA---AVKRTKISHPSSHFHH 70
           +EF Y +L  +T GFS DS +GKGS   V+K +L  G ++A   A+K + +   S  FH 
Sbjct: 490 QEFSYEELEQATGGFSEDSQVGKGSFSCVFKGILRDGTVVAVKRAIKASDVKKSSKEFH- 549

Query: 71  AHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFMPNGSL 130
                                E+++LSRL +  ++NL+G+C D   E+L+V EFM +GSL
Sbjct: 550 --------------------TELDLLSRLNHAHLLNLLGYCEDG-SERLLVYEFMAHGSL 609

Query: 131 YDLLHSR----SRPPTWNRRVRFALQVAKAVRALHA-ANPPVIHRDIKSSNVLIDGNFHA 190
           Y  LH +     +   W RRV  A+Q A+ +  LH  A PPVIHRDIKSSN+LID + +A
Sbjct: 610 YQHLHGKDPNLKKRLNWARRVTIAVQAARGIEYLHGYACPPVIHRDIKSSNILIDEDHNA 655

[Close]

BLAST of MS001127 vs. ExPASy Swiss-Prot
Match: Q9FMY3 (Serine/threonine-protein kinase-like protein At5g23170 OS=Arabidopsis thaliana OX=3702 GN=At5g23170 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.9e-30
Identity = 78/189 (41.27%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 10  IREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVL------DCGKLLAAVKRTKISHPSS 69
           ++EF Y  LV++ D FS    +GKGSHG VYKA+L      +  + + A+K      PSS
Sbjct: 1   MKEFDYDKLVTAIDRFSPSKLIGKGSHGYVYKALLHHQDVDETRQRVVAIKTPSSLSPSS 60

Query: 70  HFHHAHDYRCDINSCQFATNTPAENEIEILSRL-YNRRIVNLIGFCADAKDEKLIVVEFM 129
                       +S +       ENEI+++S L Y+  +++ +G      ++KL+VVE+M
Sbjct: 61  --------PSSSSSSKSEQTKKLENEIDVMSSLPYHPHVLSFLGHA----EKKLMVVEYM 120

Query: 130 PNGSLYDLLHSRSRP-PTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 189
           PN SLY LLH  + P PTW +R+  ALQ+A AV  LH     +IHRDIKS N+L D N+ 
Sbjct: 121 PNESLYQLLHVSTDPLPTWLKRIEIALQIASAVHFLHEHG--IIHRDIKSENILFDSNWE 175

Query: 190 ARLGDFGLA 191
           A+L DFGLA
Sbjct: 181 AKLADFGLA 175

[Close]

BLAST of MS001127 vs. ExPASy TrEMBL
Match: A0A6J1KKZ9 (serine/threonine-protein kinase-like protein At1g28390 OS=Cucurbita maxima OX=3661 GN=LOC111496191 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 4.4e-77
Identity = 154/189 (81.48%), Postives = 166/189 (87.83%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK  +PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDAGKLVAAVKRTKSPNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S+FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFC D+  EKLIVVEFM
Sbjct: 83  SNFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCVDS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRVRFALQVAKAVR LHA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVRFALQVAKAVRDLHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. ExPASy TrEMBL
Match: A0A6J1GHK7 (serine/threonine-protein kinase-like protein At3g51990 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454005 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.2e-76
Identity = 154/189 (81.48%), Postives = 165/189 (87.30%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK  +PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDAGKLVAAVKRTKSPNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFCAD+  EKLIVVEFM
Sbjct: 83  STFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCADS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRV FALQVAKAVR LHA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVLFALQVAKAVRDLHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. ExPASy TrEMBL
Match: A0A6J1GGK6 (serine/threonine-protein kinase-like protein At3g51990 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454005 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 2.2e-76
Identity = 154/189 (81.48%), Postives = 165/189 (87.30%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           KRN+P  IR F YS+LVSST+GFSAD FLGKGSHGSVYKAVLD GKL+AAVKRTK  +PS
Sbjct: 23  KRNKPFEIRHFRYSELVSSTNGFSADCFLGKGSHGSVYKAVLDAGKLVAAVKRTKSPNPS 82

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
           S FHH   YRC  +SCQFA NTPAENEIEILS + N+RIVNLIGFCAD+  EKLIVVEFM
Sbjct: 83  STFHH---YRCH-SSCQFAGNTPAENEIEILSTIRNQRIVNLIGFCADS-TEKLIVVEFM 142

Query: 123 PNGSLYDLLHSR-SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 182
           PNGSLYDLLHSR SRPP W RRV FALQVAKAVR LHA+NPPVIHRDIKSSNVLIDGNF+
Sbjct: 143 PNGSLYDLLHSRSSRPPGWTRRVLFALQVAKAVRDLHASNPPVIHRDIKSSNVLIDGNFN 202

Query: 183 ARLGDFGLA 191
           ARLGDFGLA
Sbjct: 203 ARLGDFGLA 206

[Close]

BLAST of MS001127 vs. ExPASy TrEMBL
Match: A0A5A7VAB5 (Serine/threonine-protein kinase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004480 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.3e-75
Identity = 144/188 (76.60%), Postives = 160/188 (85.11%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           K  +P  IR+F YSDL+SST+ FS D FLGKGSHG+VYKAVLD GKL+AAVKRTK ++PS
Sbjct: 33  KPKKPFQIRQFSYSDLLSSTNSFSPDCFLGKGSHGAVYKAVLDGGKLVAAVKRTKFTNPS 92

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
             FH+        NSCQ   +TPAENEIEILSRL N RIVNLIGFCAD+KDEKL+VVEFM
Sbjct: 93  PSFHY--------NSCQLPNHTPAENEIEILSRLRNPRIVNLIGFCADSKDEKLLVVEFM 152

Query: 123 PNGSLYDLLHSRSRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFHA 182
           PNGSLYDLLHS+SRPP W RR+RFALQVAKAVRALH +NPPVIHRDIKSSNVLIDGNF+A
Sbjct: 153 PNGSLYDLLHSQSRPPGWTRRLRFALQVAKAVRALHTSNPPVIHRDIKSSNVLIDGNFNA 212

Query: 183 RLGDFGLA 191
           RLGDFGLA
Sbjct: 213 RLGDFGLA 212

[Close]

BLAST of MS001127 vs. ExPASy TrEMBL
Match: A0A1S3BC62 (serine/threonine-protein kinase-like protein At3g51990 OS=Cucumis melo OX=3656 GN=LOC103488101 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.3e-75
Identity = 144/188 (76.60%), Postives = 160/188 (85.11%), Query Frame = 0


Query: 3   KRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPS 62
           K  +P  IR+F YSDL+SST+ FS D FLGKGSHG+VYKAVLD GKL+AAVKRTK ++PS
Sbjct: 33  KPKKPFQIRQFSYSDLLSSTNSFSPDCFLGKGSHGAVYKAVLDGGKLVAAVKRTKFTNPS 92

Query: 63  SHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFM 122
             FH+        NSCQ   +TPAENEIEILSRL N RIVNLIGFCAD+KDEKL+VVEFM
Sbjct: 93  PSFHY--------NSCQLPNHTPAENEIEILSRLRNPRIVNLIGFCADSKDEKLLVVEFM 152

Query: 123 PNGSLYDLLHSRSRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFHA 182
           PNGSLYDLLHS+SRPP W RR+RFALQVAKAVRALH +NPPVIHRDIKSSNVLIDGNF+A
Sbjct: 153 PNGSLYDLLHSQSRPPGWTRRLRFALQVAKAVRALHTSNPPVIHRDIKSSNVLIDGNFNA 212

Query: 183 RLGDFGLA 191
           RLGDFGLA
Sbjct: 213 RLGDFGLA 212

[Close]

BLAST of MS001127 vs. TAIR 10
Match: AT1G28390.1 (Protein kinase superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 5.0e-49
Identity = 108/195 (55.38%), Postives = 136/195 (69.74%), Query Frame = 0


Query: 1   PRKRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISH 60
           P KR+ P  +R F Y +L  +T+GFSA++FLGKGSHG VYKAVLD GKLLAAVKRT I+ 
Sbjct: 29  PEKRHPP-KLRVFNYDELAVATNGFSANNFLGKGSHGRVYKAVLDDGKLLAAVKRTTITT 88

Query: 61  PSSHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCAD-AKDEKLIVV 120
              + ++                +  +NEIEILSR+ +R +VNLIG+C D  +  KL+VV
Sbjct: 89  TVGNNNN--------------NVSQVDNEIEILSRVRHRWMVNLIGYCVDHRRKTKLLVV 148

Query: 121 EFMPNGSLYDLLHSR----SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVL 180
           E+MPNG+L+D LHSR    SR  +WNRR++ ALQ+A AV ALH A   VIHRDIKS NVL
Sbjct: 149 EYMPNGTLHDQLHSRSSLDSRLSSWNRRIKHALQIAIAVHALHTAETQVIHRDIKSCNVL 208

Query: 181 IDGNFHARLGDFGLA 191
           IDG+ +ARL DFGLA
Sbjct: 209 IDGDGNARLADFGLA 208

[Close]

BLAST of MS001127 vs. TAIR 10
Match: AT1G28390.2 (Protein kinase superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 5.0e-49
Identity = 108/195 (55.38%), Postives = 136/195 (69.74%), Query Frame = 0


Query: 1   PRKRNRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISH 60
           P KR+ P  +R F Y +L  +T+GFSA++FLGKGSHG VYKAVLD GKLLAAVKRT I+ 
Sbjct: 29  PEKRHPP-KLRVFNYDELAVATNGFSANNFLGKGSHGRVYKAVLDDGKLLAAVKRTTITT 88

Query: 61  PSSHFHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCAD-AKDEKLIVV 120
              + ++                +  +NEIEILSR+ +R +VNLIG+C D  +  KL+VV
Sbjct: 89  TVGNNNN--------------NVSQVDNEIEILSRVRHRWMVNLIGYCVDHRRKTKLLVV 148

Query: 121 EFMPNGSLYDLLHSR----SRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVL 180
           E+MPNG+L+D LHSR    SR  +WNRR++ ALQ+A AV ALH A   VIHRDIKS NVL
Sbjct: 149 EYMPNGTLHDQLHSRSSLDSRLSSWNRRIKHALQIAIAVHALHTAETQVIHRDIKSCNVL 208

Query: 181 IDGNFHARLGDFGLA 191
           IDG+ +ARL DFGLA
Sbjct: 209 IDGDGNARLADFGLA 208

[Close]

BLAST of MS001127 vs. TAIR 10
Match: AT3G51990.1 (Protein kinase superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 7.2e-40
Identity = 92/191 (48.17%), Postives = 118/191 (61.78%), Query Frame = 0


Query: 5   NRPLNIREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPSSH 64
           +RP  +R F + DL S+T GF  ++ LG+GSHGSVYKAV+  G    AVKR   S   S 
Sbjct: 40  DRP-RLRRFLHRDLESATGGFDINNLLGRGSHGSVYKAVI--GSRHIAVKRPSKSREISR 99

Query: 65  FHHAHDYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFMPN 124
             H                    NE EILSR+ + R VNL+GF AD   E L+VVEFM N
Sbjct: 100 EFH--------------------NEFEILSRIRSPRFVNLLGFSADNSKEPLLVVEFMGN 159

Query: 125 GSLYDLLHS-----RSRPPTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGN 184
           GSLYD++HS          +W++R++ ALQ+AKAV  LH+   P+IHRDIKS+NVL+D N
Sbjct: 160 GSLYDVIHSDTVLNSGAISSWSKRIKIALQIAKAVHLLHSQETPIIHRDIKSANVLMDKN 207

Query: 185 FHARLGDFGLA 191
            +A+LGDFGLA
Sbjct: 220 LNAKLGDFGLA 207

[Close]

BLAST of MS001127 vs. TAIR 10
Match: AT5G01950.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 132.9 bits (333), Expect = 2.7e-31
Identity = 76/183 (41.53%), Postives = 112/183 (61.20%), Query Frame = 0


Query: 10  IREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVLDCGKLLAAVKRTKISHPSSHFHHAH 69
           IR F + +L  +TD FS+ + +G+G +G VY+ VL     +AA+K               
Sbjct: 611 IRGFSFKELAEATDDFSSSTLVGRGGYGKVYRGVLS-DNTVAAIK--------------- 670

Query: 70  DYRCDINSCQFATNTPAENEIEILSRLYNRRIVNLIGFCADAKDEKLIVVEFMPNGSLYD 129
             R D  S Q        NEIE+LSRL++R +V+LIG+C D + E+++V EFM NG+L D
Sbjct: 671 --RADEGSLQ--GEKEFLNEIELLSRLHHRNLVSLIGYC-DEESEQMLVYEFMSNGTLRD 730

Query: 130 LLHSRSRPP-TWNRRVRFALQVAKAVRALHA-ANPPVIHRDIKSSNVLIDGNFHARLGDF 189
            L ++ +   ++  R+R AL  AK +  LH  ANPPV HRDIK+SN+L+D NF+A++ DF
Sbjct: 731 WLSAKGKESLSFGMRIRVALGAAKGILYLHTEANPPVFHRDIKASNILLDPNFNAKVADF 772

Query: 190 GLA 191
           GL+
Sbjct: 791 GLS 772

[Close]

BLAST of MS001127 vs. TAIR 10
Match: AT5G23170.1 (Protein kinase superfamily protein )

HSP 1 Score: 132.9 bits (333), Expect = 2.7e-31
Identity = 78/189 (41.27%), Postives = 110/189 (58.20%), Query Frame = 0


Query: 10  IREFPYSDLVSSTDGFSADSFLGKGSHGSVYKAVL------DCGKLLAAVKRTKISHPSS 69
           ++EF Y  LV++ D FS    +GKGSHG VYKA+L      +  + + A+K      PSS
Sbjct: 1   MKEFDYDKLVTAIDRFSPSKLIGKGSHGYVYKALLHHQDVDETRQRVVAIKTPSSLSPSS 60

Query: 70  HFHHAHDYRCDINSCQFATNTPAENEIEILSRL-YNRRIVNLIGFCADAKDEKLIVVEFM 129
                       +S +       ENEI+++S L Y+  +++ +G      ++KL+VVE+M
Sbjct: 61  --------PSSSSSSKSEQTKKLENEIDVMSSLPYHPHVLSFLGHA----EKKLMVVEYM 120

Query: 130 PNGSLYDLLHSRSRP-PTWNRRVRFALQVAKAVRALHAANPPVIHRDIKSSNVLIDGNFH 189
           PN SLY LLH  + P PTW +R+  ALQ+A AV  LH     +IHRDIKS N+L D N+ 
Sbjct: 121 PNESLYQLLHVSTDPLPTWLKRIEIALQIASAVHFLHEHG--IIHRDIKSENILFDSNWE 175

Query: 190 ARLGDFGLA 191
           A+L DFGLA
Sbjct: 181 AKLADFGLA 175

The following BLAST results are available for this feature:

BLAST of MS001127 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023536759.1	1.8e-77	82.01	serine/threonine-protein kinase-like protein At3g51990 [Cucurbita pepo subsp. pe...	[more]
XP_023002316.1	9.0e-77	81.48	serine/threonine-protein kinase-like protein At1g28390 [Cucurbita maxima]	[more]
KAG6585357.1	1.2e-76	81.48	Serine/threonine-protein kinase-like protein, partial [Cucurbita argyrosperma su...	[more]
KAG7020269.1	2.0e-76	81.48	Serine/threonine-protein kinase-like protein, partial [Cucurbita argyrosperma su...	[more]
XP_022951039.1	4.5e-76	81.48	serine/threonine-protein kinase-like protein At3g51990 isoform X2 [Cucurbita mos...	[more]

BLAST of MS001127 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGN7	7.0e-48	55.38	Serine/threonine-protein kinase-like protein At1g28390 OS=Arabidopsis thaliana O...	[more]
Q9SV05	1.0e-38	48.17	Serine/threonine-protein kinase-like protein At3g51990 OS=Arabidopsis thaliana O...	[more]
O24585	2.1e-31	41.49	Putative receptor protein kinase CRINKLY4 OS=Zea mays OX=4577 GN=CR4 PE=2 SV=1	[more]
Q75J39	1.0e-30	40.96	Serine/threonine-protein kinase-like protein CR4 OS=Oryza sativa subsp. japonica...	[more]
Q9FMY3	3.9e-30	41.27	Serine/threonine-protein kinase-like protein At5g23170 OS=Arabidopsis thaliana O...	[more]

BLAST of MS001127 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KKZ9	4.4e-77	81.48	serine/threonine-protein kinase-like protein At1g28390 OS=Cucurbita maxima OX=36...	[more]
A0A6J1GHK7	2.2e-76	81.48	serine/threonine-protein kinase-like protein At3g51990 isoform X2 OS=Cucurbita m...	[more]
A0A6J1GGK6	2.2e-76	81.48	serine/threonine-protein kinase-like protein At3g51990 isoform X1 OS=Cucurbita m...	[more]
A0A5A7VAB5	5.3e-75	76.60	Serine/threonine-protein kinase-like protein OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3BC62	5.3e-75	76.60	serine/threonine-protein kinase-like protein At3g51990 OS=Cucumis melo OX=3656 G...	[more]

BLAST of MS001127 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G28390.1	5.0e-49	55.38	Protein kinase superfamily protein	[more]
AT1G28390.2	5.0e-49	55.38	Protein kinase superfamily protein	[more]
AT3G51990.1	7.2e-40	48.17	Protein kinase superfamily protein	[more]
AT5G01950.1	2.7e-31	41.53	Leucine-rich repeat protein kinase family protein	[more]
AT5G23170.1	2.7e-31	41.27	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 25..190 e-value: 7.3E-6 score: -52.3
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 28..190 e-value: 2.7E-26 score: 92.5
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 25..190 score: 26.788889
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 122..190 e-value: 4.2E-24 score: 87.5
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 9..121 e-value: 1.2E-18 score: 69.2
None	No IPR available	PIRSR	PIRSR038172-1	PIRSR038172-1	coord: 31..190 e-value: 5.6E-8 score: 29.4
None	No IPR available	PANTHER	PTHR47989:SF5	SUBFAMILY NOT NAMED	coord: 3..190
None	No IPR available	PANTHER	PTHR47989	OS01G0750732 PROTEIN	coord: 3..190
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 31..54
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 164..176
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 8..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001127.1	MS001127.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity