MS001124 (gene) Bitter gourd (TR) v1

BLAST of MS001124 vs. NCBI nr
Match: XP_022131300.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 520.8 bits (1340), Expect = 6.8e-144
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL
Sbjct: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL
Sbjct: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS
Sbjct: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM
Sbjct: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240

Query: 241 EGFIERISQTLAETEA 257
           E FIERISQTLAETEA
Sbjct: 241 ESFIERISQTLAETEA 256

BLAST of MS001124 vs. NCBI nr
Match: XP_022961839.1 (dihydrofolate reductase-like [Cucurbita moschata])

HSP 1 Score: 411.0 bits (1055), Expect = 7.6e-111
Identity = 200/255 (78.43%), Postives = 221/255 (86.67%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSS SP L S       P S+SRSP+   +R  IMG++ +I+RKPRFLCLHGFRTSG IL
Sbjct: 1   MSSLSPLLCS-------PISKSRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWP SVL+  DL FLDAPFPAEGKSD+EGIFDPPYFEWFQFS +  EY+NFDECL
Sbjct: 61  KKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ KGIALTKVPKIKFV+IVSGAKFRS S
Sbjct: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQS 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           +AEKAYSP IGCPSLHFLG EDFLMPHG KLLESYIEP++INHPKGHT+PRLD++GL+VM
Sbjct: 181 VAEKAYSPVIGCPSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVM 240

Query: 241 EGFIERISQTLAETE 256
           E FIE+IS+ L E E
Sbjct: 241 ESFIEKISKMLDEAE 248

BLAST of MS001124 vs. NCBI nr
Match: XP_023545459.1 (dihydrofolate reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 408.7 bits (1049), Expect = 3.8e-110
Identity = 200/255 (78.43%), Postives = 220/255 (86.27%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSS SP L S       P S+ RSP+   +R  IMG++ +I+RKPRFLCLHGFRTSG IL
Sbjct: 1   MSSLSPLLCS-------PISKYRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWP SVL+  DL FLDAPFPAEGKSD+EGIFDPPYFEWFQFS +  EY+NFDECL
Sbjct: 61  KKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ KGIALTKVPKIKFV+IVSGAKFRS S
Sbjct: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQS 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           +AEKAYS +IGCPSLHFLG EDFLMPHG KLLESYIEP+VINHPKGHT+PRLD+KGL+VM
Sbjct: 181 VAEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVM 240

Query: 241 EGFIERISQTLAETE 256
           E FIE+IS+ L E E
Sbjct: 241 ESFIEKISKMLDEAE 248

BLAST of MS001124 vs. NCBI nr
Match: XP_038885360.1 (dihydrofolate reductase-like [Benincasa hispida])

HSP 1 Score: 407.9 bits (1047), Expect = 6.4e-110
Identity = 196/241 (81.33%), Postives = 212/241 (87.97%), Query Frame = 0

Query: 15  TPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDN 74
           +P P   + S      R  IMG++AKI RKPRFLCLHGFRTS  ILKKQVGKWP+SVLD 
Sbjct: 3   SPFPSLHTNS------RIPIMGTDAKIHRKPRFLCLHGFRTSAAILKKQVGKWPASVLDQ 62

Query: 75  FDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFD 134
           FDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFS +  EY+NFDECLSFIEDYMLKHGPFD
Sbjct: 63  FDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSQEFTEYRNFDECLSFIEDYMLKHGPFD 122

Query: 135 GFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPS 194
           GFLGFSQGAILSAALPG Q KGIALTKVPKIKFV+IVSGAKFRS S+A+KAYS  IGCPS
Sbjct: 123 GFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAQKAYSTPIGCPS 182

Query: 195 LHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVMEGFIERISQTLAET 254
           LHFLG+EDFLMPHG KLLESY+EP VINHPKGHTVPRLD+KGL+VME FI+RIS+TL ET
Sbjct: 183 LHFLGEEDFLMPHGQKLLESYVEPTVINHPKGHTVPRLDDKGLEVMESFIKRISKTLDET 237

Query: 255 E 256
           E
Sbjct: 243 E 237

BLAST of MS001124 vs. NCBI nr
Match: XP_022997250.1 (dihydrofolate reductase-like [Cucurbita maxima])

HSP 1 Score: 407.5 bits (1046), Expect = 8.4e-110
Identity = 198/255 (77.65%), Postives = 221/255 (86.67%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSS SP L S       P S++RSP+   +R  IMG++ +I+RKPRFLCLHGFRTSG IL
Sbjct: 1   MSSLSPLLCS-------PISKTRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWP SVL+  DL FLDAPFPAEGKSD+EGIFDPPYFEWFQFS +  EY+NFDECL
Sbjct: 61  KKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ KG+ALTKVPKIKFV+IVSGAKFRS S
Sbjct: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQS 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           +AEKAYS +IGCPSLHFLG EDFLMPHG KLLESYIEP+VINHPKGHT+PRLD++GL+VM
Sbjct: 181 VAEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVM 240

Query: 241 EGFIERISQTLAETE 256
           E FIE+IS+ L E E
Sbjct: 241 ESFIEKISKMLDEAE 248

BLAST of MS001124 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 102.4 bits (254), Expect = 7.5e-21
Identity = 66/203 (32.51%), Postives = 96/203 (47.29%), Query Frame = 0

Query: 46  RFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSD------------VE 105
           + LCLHG+  SG +  K++G     +    +LHF   P  A+ ++D            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 106 GIFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 165
           G  +   F WF+       Y ++DE L  I  YM + GPFDG +GFSQGA + A L  + 
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 166 EKG---IALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFLGDEDFLMP--HG 225
           + G       + P  KFVV V G +   P   +  Y+P +  PSLH  G  D L+P    
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGGFRAEKPEF-DHFYNPKLTTPSLHIAGTSDTLVPLARS 185

Query: 226 LKLLESYIEPLVINHPKGHTVPR 232
            +L+E      V+ HP  H VP+
Sbjct: 186 KQLVERCENAHVLLHPGQHIVPQ 207

BLAST of MS001124 vs. ExPASy Swiss-Prot
Match: Q9VDL1 (Esterase CG5412 OS=Drosophila melanogaster OX=7227 GN=CG5412 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.5e-16
Identity = 72/239 (30.13%), Postives = 109/239 (45.61%), Query Frame = 0

Query: 31  RASIMGSEAKIQ--RKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEG 90
           RAS    + K++   K R LCLHG+R +G+  K ++G +        +  F+ AP  A+ 
Sbjct: 15  RASSSKQQLKLEITEKVRVLCLHGYRQNGEAFKNKLGSFRKFANKYAEFVFITAPHVAKA 74

Query: 91  -KSDVEGIFDPPYFEWFQFSAD-MMEYQN-------FDECLSFIEDYMLKHGPFDGFLGF 150
            +S  E +  P    W+    D   +  N       F E L  +E+     GPF G LGF
Sbjct: 75  LESAAEPV--PEQRSWWANKDDGSFKGTNKGGPAFGFQESLRCVEEAWRTQGPFQGLLGF 134

Query: 151 SQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPSLAE-KAYSPSIGCPSLHFL 210
           SQGA     + GL +K   LT + + +F V+ SG  F S SL    AY  +I  P+LH  
Sbjct: 135 SQGACFVGLICGLAKK--KLTSI-RPEFAVLASG--FLSGSLVHMSAYEEAISIPTLHIY 194

Query: 211 GDEDFLMPHGL--KLLESYIEPLVINHPKGHTVPRLDEKGLKVMEGFIERISQTLAETE 256
           G  D ++P  +   L   +    V+ H  GH  P   ++    +  F +R+ + L   E
Sbjct: 195 GQTDEIIPKEMSESLAARFKNAEVLEHSGGHYFPATAQQKQTFINFFQDRLQEYLEHEE 246

BLAST of MS001124 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.6e-16
Identity = 69/219 (31.51%), Postives = 98/219 (44.75%), Query Frame = 0

Query: 42  QRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPF----------PAEGKSD 101
           +RK R LCLHGFR +    K + G     + +  +L F+DAP           P  G  +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 102 ------VEGIFDPPY---FEWFQFSADMMEYQ----NFDECLSFIEDYMLKHGPFDGFLG 161
                 V   FD P    +   Q   D ++YQ     FD+ L++++    + GPFDG LG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILG 470

Query: 162 FSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFL 221
           FSQGA ++AA+ G QE+   L      +F V+ SG  F    L E     SI CPSLH  
Sbjct: 471 FSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIF 530

Query: 222 G-----DEDFLMPHGLKLLESYIE--PLVINHPKGHTVP 231
           G     D   +      L   + +    ++ H  GH +P
Sbjct: 531 GSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

BLAST of MS001124 vs. ExPASy Swiss-Prot
Match: Q29BR3 (Esterase GA18864 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA18864 PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.2e-15
Identity = 72/239 (30.13%), Postives = 109/239 (45.61%), Query Frame = 0

Query: 31  RASIMGSEAKIQ--RKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAP-FPAE 90
           RAS    + K++   K R LCLHG+R  G   K ++G +        +  F+ AP   A 
Sbjct: 15  RASSSKQQPKLEITEKVRVLCLHGYRQDGDAFKNKLGSFRKFTSKYAEFVFISAPHIAAP 74

Query: 91  GKSDVEGIFDPPYFEWFQFSAD-MMEYQN-------FDECLSFIEDYMLKHGPFDGFLGF 150
            +S  E +  P    W+    D   +  N       F + L  +E+     GPF G LGF
Sbjct: 75  LESAAEPV--PEQRSWWANKDDGTFKGTNKGGPAFGFQDSLRLVEEAWKTQGPFQGLLGF 134

Query: 151 SQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPSLAE-KAYSPSIGCPSLHFL 210
           SQGA     + GL +K   LT + + +F V+ SG  F S SL    AY   +  P+LH  
Sbjct: 135 SQGACFVGLICGLAKK--KLTSI-RPEFAVLSSG--FVSGSLVHMSAYEEPVSIPTLHIY 194

Query: 211 GDEDFLMPHGLK-LLESYIEPL-VINHPKGHTVPRLDEKGLKVMEGFIERISQTLAETE 256
           G  D ++P  +  LL S+ + + V+ H  GH  P   ++    +  F +R+ + L   E
Sbjct: 195 GSSDEIIPKDMSALLASHFKNVEVLEHGGGHYFPATAQQKQTYINFFQDRLQEYLEHLE 246

BLAST of MS001124 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.1e-15
Identity = 61/204 (29.90%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 46  RFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGI---------F 105
           R LCL GFR S +  +++ G    ++    +L  L  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 106 DPPYFEWF-QFSADMM-------EYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 165
           + P   WF +  AD+          +  +E L  +   + K GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 166 LPGLQEKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFLGDEDFLMP-- 225
           +  L + G    + P  +FV++VSG   R   L E      +  PSLH  GD D ++P  
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGDTDGVIPSQ 187

Query: 226 HGLKLLESYIEPLVINHPKGHTVP 231
             ++L   +   + + H  GH +P
Sbjct: 188 ESMQLCSRFDGAVTLTHSGGHFIP 209

BLAST of MS001124 vs. ExPASy TrEMBL
Match: A0A6J1BPA7 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111004563 PE=4 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.3e-144
Identity = 255/256 (99.61%), Postives = 255/256 (99.61%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL
Sbjct: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL
Sbjct: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS
Sbjct: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM
Sbjct: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240

Query: 241 EGFIERISQTLAETEA 257
           E FIERISQTLAETEA
Sbjct: 241 ESFIERISQTLAETEA 256

BLAST of MS001124 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.7e-111
Identity = 200/255 (78.43%), Postives = 221/255 (86.67%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSS SP L S       P S+SRSP+   +R  IMG++ +I+RKPRFLCLHGFRTSG IL
Sbjct: 1   MSSLSPLLCS-------PISKSRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWP SVL+  DL FLDAPFPAEGKSD+EGIFDPPYFEWFQFS +  EY+NFDECL
Sbjct: 61  KKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ KGIALTKVPKIKFV+IVSGAKFRS S
Sbjct: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQS 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           +AEKAYSP IGCPSLHFLG EDFLMPHG KLLESYIEP++INHPKGHT+PRLD++GL+VM
Sbjct: 181 VAEKAYSPVIGCPSLHFLGKEDFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVM 240

Query: 241 EGFIERISQTLAETE 256
           E FIE+IS+ L E E
Sbjct: 241 ESFIEKISKMLDEAE 248

BLAST of MS001124 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 4.0e-110
Identity = 198/255 (77.65%), Postives = 221/255 (86.67%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSS SP L S       P S++RSP+   +R  IMG++ +I+RKPRFLCLHGFRTSG IL
Sbjct: 1   MSSLSPLLCS-------PISKTRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWP SVL+  DL FLDAPFPAEGKSD+EGIFDPPYFEWFQFS +  EY+NFDECL
Sbjct: 61  KKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ KG+ALTKVPKIKFV+IVSGAKFRS S
Sbjct: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQS 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           +AEKAYS +IGCPSLHFLG EDFLMPHG KLLESYIEP+VINHPKGHT+PRLD++GL+VM
Sbjct: 181 VAEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVM 240

Query: 241 EGFIERISQTLAETE 256
           E FIE+IS+ L E E
Sbjct: 241 ESFIEKISKMLDEAE 248

BLAST of MS001124 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 1.1e-107
Identity = 196/255 (76.86%), Postives = 212/255 (83.14%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           MSSF P L S Q  + I       PQ  F    IMGS+ KI  KPRFLCLHGFRTS  IL
Sbjct: 1   MSSFFPSLSSHQFLSTI-------PQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWP SVLD  DLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFS +  EY+NFDECL
Sbjct: 61  KKQVGKWPPSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIE+YM++HGPFDGFLGFSQGAILSAALPG Q KGIALTKVPKIKFV+IVSGAKFRS S
Sbjct: 121 SFIENYMIEHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSES 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           +AEKAYS  IGCPSLHFLG+EDFLMPHG KLLESYIEP +I HPKGHT+PRLD+K L+VM
Sbjct: 181 VAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVM 240

Query: 241 EGFIERISQTLAETE 256
           E FI+RIS+ L ETE
Sbjct: 241 ESFIQRISKMLNETE 248

BLAST of MS001124 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.0e-105
Identity = 194/255 (76.08%), Postives = 207/255 (81.18%), Query Frame = 0

Query: 1   MSSFSPPLRSAQLHTPIPESESRSPQIVFKRASIMGSEAKIQRKPRFLCLHGFRTSGQIL 60
           M  F P L S Q  T I       PQ  F    IMGS+ K   KPRFLCLHGFRTS  IL
Sbjct: 1   MPPFFPSLSSHQSLTTI-------PQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAIL 60

Query: 61  KKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSADMMEYQNFDECL 120
           KKQVGKWP SVLD  DLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFS +  EY+NFDECL
Sbjct: 61  KKQVGKWPLSVLDQLDLHFLDAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECL 120

Query: 121 SFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPS 180
           SFIE+YM+KHGPFDGFLGFSQGAILSAALPG Q KGIALTKVPKIKFV+IVSGAKFRS S
Sbjct: 121 SFIENYMIKHGPFDGFLGFSQGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSES 180

Query: 181 LAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIEPLVINHPKGHTVPRLDEKGLKVM 240
           +AEKAYS  IGCPSLHFLG+EDFLMPHG KLLESYIEP +I HPKGHT+PRLD+K L+VM
Sbjct: 181 VAEKAYSTPIGCPSLHFLGEEDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVM 240

Query: 241 EGFIERISQTLAETE 256
           E FI RIS+ L E E
Sbjct: 241 ESFIHRISKILNENE 248

BLAST of MS001124 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 309.3 bits (791), Expect = 2.9e-84
Identity = 143/222 (64.41%), Postives = 175/222 (78.83%), Query Frame = 0

Query: 35  MGSEAK-IQRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVE 94
           MGSE + I RKPRFLCLHGFRTSG+I+K Q+ KWP SV+D  DL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 95  GIFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 154
           GIFDPPY+EWFQF+ +  EY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 155 EKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLE 214
            KGIA  KVPKIKFV+I+ GAK +S  LAE AYS S+   SLHFLG+ DFL P+G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 215 SYIEPLVINHPKGHTVPRLDEKGLKVMEGFIERISQTLAETE 256
           SY  P+V++HPKGHTVPRLDEK L+ +  FI+ +   + E +
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEED 222

BLAST of MS001124 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 7.1e-75
Identity = 126/216 (58.33%), Postives = 164/216 (75.93%), Query Frame = 0

Query: 43  RKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEGIFDPPYFE 102
           + PR LCLHGFRTSG+IL+  +GKWP ++L + DL FLDAPFPA GKSDVE  FDPPY+E
Sbjct: 28  KNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVERFFDPPYYE 87

Query: 103 WFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQEKGIALTKV 162
           W+Q +    EY+NF+ECL++IEDYM+K+GPFDG LGFSQGA L+AA+PG+QE+G ALTKV
Sbjct: 88  WYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGSALTKV 147

Query: 163 PKIKFVVIVSGAK-----FRSPSLAEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLESYIE 222
           PK+KF+VI+SGAK     F  P  A  A+S  + CPSLHF+G+ DFL   G  L+ES++E
Sbjct: 148 PKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEVLVESFVE 207

Query: 223 PLVINHPKGHTVPRLDEKGLKVMEGFIERISQTLAE 254
           P+VI+H  GH +P+LD K  + M  F + I Q L++
Sbjct: 208 PVVIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSD 243

BLAST of MS001124 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 245.4 bits (625), Expect = 5.1e-65
Identity = 115/166 (69.28%), Postives = 134/166 (80.72%), Query Frame = 0

Query: 35  MGSEAK-IQRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVE 94
           MGSE + I RKPRFLCLHGFRTSG+I+K Q+ KWP SV+D  DL FLDAPFP +GKSDVE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 95  GIFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 154
           GIFDPPY+EWFQF+ +  EY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 155 EKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFLG 200
            KGIA  KVPKIKFV+I+ GAK +S  LAE AYS S+   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

BLAST of MS001124 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 86.3 bits (212), Expect = 4.0e-17
Identity = 69/219 (31.51%), Postives = 98/219 (44.75%), Query Frame = 0

Query: 42  QRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPF----------PAEGKSD 101
           +RK R LCLHGFR +    K + G     + +  +L F+DAP           P  G  +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 102 ------VEGIFDPPY---FEWFQFSADMMEYQ----NFDECLSFIEDYMLKHGPFDGFLG 161
                 V   FD P    +   Q   D ++YQ     FD+ L++++    + GPFDG LG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILG 470

Query: 162 FSQGAILSAALPGLQEKGIALTKVPKIKFVVIVSGAKFRSPSLAEKAYSPSIGCPSLHFL 221
           FSQGA ++AA+ G QE+   L      +F V+ SG  F    L E     SI CPSLH  
Sbjct: 471 FSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIF 530

Query: 222 G-----DEDFLMPHGLKLLESYIE--PLVINHPKGHTVP 231
           G     D   +      L   + +    ++ H  GH +P
Sbjct: 531 GSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564