MS001052 (gene) Bitter gourd (TR) v1

BLAST of MS001052 vs. NCBI nr
Match: XP_022131518.1 (DNA gyrase subunit A, chloroplastic/mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 1742.6 bits (4512), Expect = 0.0e+00
Identity = 904/911 (99.23%), Postives = 905/911 (99.34%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSRGGED 60
           MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTS+STSFRSIRLLSSRGGE 
Sbjct: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSTSTSFRSIRLLSSRGGEV 60

Query: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120
           GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG
Sbjct: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120

Query: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180
           LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP
Sbjct: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180

Query: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240
           AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA
Sbjct: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240

Query: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300
           VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT
Sbjct: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300

Query: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360
           GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD
Sbjct: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360

Query: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420
           ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF
Sbjct: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420

Query: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480
           LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF
Sbjct: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480

Query: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540
           SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE
Sbjct: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540

Query: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600
           LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK
Sbjct: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600

Query: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660
           SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD
Sbjct: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660

Query: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720
           GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR
Sbjct: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720

Query: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780
           CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE
Sbjct: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780

Query: 781 RMPTTPR-----FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVF 840
           RMPTTPR     FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVF
Sbjct: 781 RMPTTPRSSSKGFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVF 840

Query: 841 VVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLI 900
           VVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLI
Sbjct: 841 VVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLI 900

Query: 901 SAMDTEPEEEA 907
           SAMDTEPEEEA
Sbjct: 901 SAMDTEPEEEA 911

BLAST of MS001052 vs. NCBI nr
Match: XP_022131517.1 (DNA gyrase subunit A, chloroplastic/mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 1740.7 bits (4507), Expect = 0.0e+00
Identity = 904/916 (98.69%), Postives = 905/916 (98.80%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSRGGED 60
           MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTS+STSFRSIRLLSSRGGE 
Sbjct: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSTSTSFRSIRLLSSRGGEV 60

Query: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120
           GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG
Sbjct: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120

Query: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180
           LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP
Sbjct: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180

Query: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240
           AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA
Sbjct: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240

Query: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300
           VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT
Sbjct: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300

Query: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360
           GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD
Sbjct: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360

Query: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420
           ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF
Sbjct: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420

Query: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480
           LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF
Sbjct: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480

Query: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540
           SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE
Sbjct: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540

Query: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600
           LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK
Sbjct: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600

Query: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660
           SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD
Sbjct: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660

Query: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720
           GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR
Sbjct: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720

Query: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780
           CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE
Sbjct: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780

Query: 781 RMPTTPR----------FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDR 840
           RMPTTPR          FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDR
Sbjct: 781 RMPTTPRSSFGVSSSKGFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDR 840

Query: 841 LAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQ 900
           LAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQ
Sbjct: 841 LAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQ 900

Query: 901 SASLISAMDTEPEEEA 907
           SASLISAMDTEPEEEA
Sbjct: 901 SASLISAMDTEPEEEA 916

BLAST of MS001052 vs. NCBI nr
Match: XP_038885119.1 (DNA gyrase subunit A, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1629.4 bits (4218), Expect = 0.0e+00
Identity = 849/927 (91.59%), Postives = 878/927 (94.71%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSR---- 60
           MASSSGLRISSLLRHQ  PPSVSNRF RICHGLSELRFL T++STS RSIRL+ SR    
Sbjct: 1   MASSSGLRISSLLRHQLAPPSVSNRFVRICHGLSELRFLSTANSTSSRSIRLVKSRRRDE 60

Query: 61  ----GGEDGGGGG--------DGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDG 120
               GG DG  G          G +GR+VH ELHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  PVKEGGTDGQDGNGSLTVKDRGGSDGRIVHAELHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLL 240
           PLIQGHGNFGSIDADP AAMRYTECRLEAL EAMLL+DLELNTVDFVPNFDNSQ+EPSLL
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALAEAMLLSDLELNTVDFVPNFDNSQKEPSLL 240

Query: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPT 300
           PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLI NPEATLQELLEYMPGPDFPT
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIA 360
           GGLIMGNNGILEAYRTGRGR+TVRGK DVELLDLKTKRTA+IIKE+PYQTNKSALVEKIA
Sbjct: 301 GGLIMGNNGILEAYRTGRGRVTVRGKIDVELLDLKTKRTAIIIKEIPYQTNKSALVEKIA 360

Query: 361 ELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGII 420
           ELVENKTLDG+SDIRDESDRSGMRIVIELKRGADPLIIQNNL+RLTSLQS FSCNMVGII
Sbjct: 361 ELVENKTLDGISDIRDESDRSGMRIVIELKRGADPLIIQNNLFRLTSLQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIR 480
           NGQPKLMGLKELLQAFLDFRCSVVERRARFKL QA+ERRHIVEGIVIGLDNLDGVI+LIR
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLSQAQERRHIVEGIVIGLDNLDGVIHLIR 480

Query: 481 EASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELL 540
           EASSNSIASA+LRT+F+LSEKQAEAILDINLRRLTHLERKKFIDESKSLME+ISKL+ELL
Sbjct: 481 EASSNSIASASLRTQFNLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELL 540

Query: 541 SSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKR 600
           SSRKNILQLIEQEAIELK+KFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKR
Sbjct: 541 SSRKNILQLIEQEAIELKNKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKR 600

Query: 601 MKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECA 660
           MKP+TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSARAYKIPEC 
Sbjct: 601 MKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSARAYKIPECG 660

Query: 661 RTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGI 720
           RTATGTPLVQILSLSDGERITSIIPVSEFDG+QFLLMLTANGYIKKVSLN FS+IR+TGI
Sbjct: 661 RTATGTPLVQILSLSDGERITSIIPVSEFDGEQFLLMLTANGYIKKVSLNFFSAIRTTGI 720

Query: 721 IAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780
           IAIQLVSGDELKWV  C ND+L+AMASQNGMVILSSCDIIR+QGRNTRGAVAMRLKAGDK
Sbjct: 721 IAIQLVSGDELKWVCCCTNDNLVAMASQNGMVILSSCDIIRSQGRNTRGAVAMRLKAGDK 780

Query: 781 MASMDIIPAAVRNNLERMPTTPR-----FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVG 840
           MASMDIIPAAV N+LE+MPTTPR     FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVG
Sbjct: 781 MASMDIIPAAVWNDLEKMPTTPRGSSKGFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVG 840

Query: 841 LIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVI 900
           LIGYKFS EDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVI
Sbjct: 841 LIGYKFSLEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVI 900

Query: 901 LMRLDHAGKIQSASLISAMDTEPEEEA 907
           LMRLDHAGKIQSASLISA DTEPEEEA
Sbjct: 901 LMRLDHAGKIQSASLISATDTEPEEEA 927

BLAST of MS001052 vs. NCBI nr
Match: XP_022961731.1 (DNA gyrase subunit A, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 1583.2 bits (4098), Expect = 0.0e+00
Identity = 824/927 (88.89%), Postives = 864/927 (93.20%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSR---- 60
           MA SSGLR SSLLRHQF PP    RF RI HGLSELRFL TS+S S RSIRL+ SR    
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHAWTRFGRIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  -------GGEDGGGG-----GDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDG 120
                  GG+DG G        G +GR+VH  LHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIVKDRGGSDGRIVHALLHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLL 240
           PLIQGHGNFGSIDADP AAMRYTECRLEALTEAMLL+DLEL+TVDFVPNFD+SQ+EPSLL
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELDTVDFVPNFDSSQKEPSLL 240

Query: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPT 300
           PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLI NPEATLQELLEYMPGPDFPT
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIA 360
           GGLIMGNNGILEAYRTGRGRITVRGKTD+ELLDLKTKRTAVII E+PYQTNKSALVEKIA
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDIELLDLKTKRTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGII 420
           ELVENKTLDG++DIRDESDRSGMRIV+ELKRGADPLIIQNNLYRLT+LQS FSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIR 480
           NGQPKLMGLKELLQAFLDFRCSVVERRARFKL QA+ERRHIVEGIV GLDNLD VI LIR
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRHIVEGIVTGLDNLDEVIQLIR 480

Query: 481 EASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELL 540
           EAS NS+ASANLR +FSLSEKQAEAILDINLRRLTHLERKKFIDESKSLME+ISKL+ELL
Sbjct: 481 EASRNSVASANLRAQFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELL 540

Query: 541 SSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKR 600
           SSRKNILQ+IEQEAIELK+KFPSPRRSIL+DTDSGQLEDIDVIPN+EMLLALSEKGYVKR
Sbjct: 541 SSRKNILQVIEQEAIELKNKFPSPRRSILKDTDSGQLEDIDVIPNDEMLLALSEKGYVKR 600

Query: 601 MKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECA 660
           MKP+TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYS+RAYKIPEC 
Sbjct: 601 MKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSRAYKIPECG 660

Query: 661 RTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGI 720
           RTATGTP++QILSLS+GERITSIIPVSEF  DQFLLMLTANGYIKKVSLN FSSIR+TGI
Sbjct: 661 RTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFFSSIRTTGI 720

Query: 721 IAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780
           IAIQLVSGDELKWVR C ND+L+AMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK
Sbjct: 721 IAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780

Query: 781 MASMDIIPAAVRNNLERMPTTPR-----FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVG 840
           MASMDIIPA++ N+ ER+PTTPR      NGPWLLFVSESGFGKRVPLSSFRLSPL RVG
Sbjct: 781 MASMDIIPASMWNDSERIPTTPRSSAKGSNGPWLLFVSESGFGKRVPLSSFRLSPLKRVG 840

Query: 841 LIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVI 900
           LIGYKFS EDRLAAVFVVGF+LAEDGESDEQVVLVSQSGTVNRIKVRD+SIQSRYARGVI
Sbjct: 841 LIGYKFSLEDRLAAVFVVGFTLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRYARGVI 900

Query: 901 LMRLDHAGKIQSASLISAMDTEPEEEA 907
           LMRLD+AGKIQSASLISA DTEPEEEA
Sbjct: 901 LMRLDYAGKIQSASLISATDTEPEEEA 927

BLAST of MS001052 vs. NCBI nr
Match: KAG6598657.1 (DNA gyrase subunit A, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1580.5 bits (4091), Expect = 0.0e+00
Identity = 823/927 (88.78%), Postives = 863/927 (93.10%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSR---- 60
           MA SSGLR SSLLRHQF PP    RF RI HGLSELRFL TS+S S RSIRL+ SR    
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHAWTRFGRIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  -------GGEDGGGG-----GDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDG 120
                  GG+DG G        G +GR+VH  LHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGIGGQDGNGSVIVKDRGGSDGRIVHALLHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLL 240
           PLIQGHGNFGSIDADP AAMRYTECRLEALTEAMLL+DLEL+TVDFVPNFD+SQ+EPSLL
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELDTVDFVPNFDSSQKEPSLL 240

Query: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPT 300
           PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLI NPEATLQELLEYMPGPDFPT
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIA 360
           GGLIMGNNGILEAYRTGRGRITVRGKTD+ELLDLKTKRTAVII E+PYQTNKSALVEKIA
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDIELLDLKTKRTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGII 420
           ELVENKTLDG++DIRDESDRSGMRIV+ELKRGADPLIIQNNLYRLT+LQS FSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIR 480
           NGQPKLMGLKELLQAFLDFRCSVVERRARFKL QA+ERRHIVEGIV GLDNLD VI LIR
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRHIVEGIVTGLDNLDEVIQLIR 480

Query: 481 EASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELL 540
           EAS NS+ASANLR +FSLSEKQAEAILDINLRRLTHLERKKFIDESKSLME+ISKL+ELL
Sbjct: 481 EASRNSVASANLRAQFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELL 540

Query: 541 SSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKR 600
           SSRKNILQ+IEQEAIELK+KFPSPRRSIL+DTDSGQLEDIDVIPN+EMLLALSEKGYVKR
Sbjct: 541 SSRKNILQVIEQEAIELKNKFPSPRRSILKDTDSGQLEDIDVIPNDEMLLALSEKGYVKR 600

Query: 601 MKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECA 660
           MKP+TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYS+RAYKIPEC 
Sbjct: 601 MKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSRAYKIPECG 660

Query: 661 RTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGI 720
           RTATGTP++QILSLS+GERITSIIPVSEF  DQFLLMLTANGYIKKVSLN FSSIR+TGI
Sbjct: 661 RTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFFSSIRTTGI 720

Query: 721 IAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780
           IAIQLVSGDELKWVR C ND+L+AMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK
Sbjct: 721 IAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780

Query: 781 MASMDIIPAAVRNNLERMPTTP-----RFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVG 840
           MASMDIIPA++ N+ ER+PTTP       NGPWLLFVSESGFGKRVPLSSFRLSPL RVG
Sbjct: 781 MASMDIIPASMWNDSERIPTTPLSSAKGSNGPWLLFVSESGFGKRVPLSSFRLSPLKRVG 840

Query: 841 LIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVI 900
           LIGYKFS EDRLAAVFVVGF+LAEDGESDEQVVLVSQSGTVNRIKVRD+SIQSRYARGVI
Sbjct: 841 LIGYKFSLEDRLAAVFVVGFTLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRYARGVI 900

Query: 901 LMRLDHAGKIQSASLISAMDTEPEEEA 907
           LMRLD+AGKIQSASLISA DTEPEEEA
Sbjct: 901 LMRLDYAGKIQSASLISATDTEPEEEA 927

BLAST of MS001052 vs. ExPASy Swiss-Prot
Match: Q5YLB5 (DNA gyrase subunit A, chloroplastic/mitochondrial OS=Nicotiana benthamiana OX=4100 GN=GYRA PE=2 SV=1)

HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 715/904 (79.09%), Postives = 795/904 (87.94%), Query Frame = 0

Query: 24  NRFPRICHGLSELRFLPTSSSTSFRSIRLLSSRGGEDGGGGGDGGNG------------- 83
           +RF  +    SELRFL + +    + +R +S+R  E+    GD GNG             
Sbjct: 27  SRFSGLRKTSSELRFLSSVTPPPRKQLRPVSARRKEE--EVGDEGNGSVILRDRGENEDR 86

Query: 84  ----RVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELGLSSRKP 143
               RVV  ELHKEAT+AYM+YAMSVLLGRALPDVRDGLKPVHRRIL+AMHELGLSS+KP
Sbjct: 87  NGGERVVLTELHKEATEAYMSYAMSVLLGRALPDVRDGLKPVHRRILYAMHELGLSSKKP 146

Query: 144 FKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADPAAAMRY 203
           +KKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR PLI+GHGNFGSIDADP AAMRY
Sbjct: 147 YKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRSPLIRGHGNFGSIDADPPAAMRY 206

Query: 204 TECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIAVGMATN 263
           TECRLEALTE+MLLADLE NTVDFVPNFDNSQ+EPSLLPAR+P LLLNG+SGIAVGMATN
Sbjct: 207 TECRLEALTESMLLADLEQNTVDFVPNFDNSQKEPSLLPARVPNLLLNGASGIAVGMATN 266

Query: 264 IPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRTGRGRIT 323
           IPPHNLGELVDAL  LI NPEATLQELLEYMPGPDFPTGG+IMGN GILEA+RTGRGR+ 
Sbjct: 267 IPPHNLGELVDALSALIHNPEATLQELLEYMPGPDFPTGGIIMGNIGILEAFRTGRGRVV 326

Query: 324 VRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRDESDRSG 383
           +RGKTD+ELLD KTKR A+II+E+PYQTNK++LVEKIA+LVENK L+GVSDIRDESDRSG
Sbjct: 327 IRGKTDIELLDSKTKRAAIIIQEIPYQTNKASLVEKIADLVENKILEGVSDIRDESDRSG 386

Query: 384 MRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAFLDFRCS 443
           MRIVIELKRG+DP I+ NNLYRLT+LQS FSCNMVGI+NGQPKLMGLKELLQAFLDFRCS
Sbjct: 387 MRIVIELKRGSDPAIVLNNLYRLTALQSSFSCNMVGILNGQPKLMGLKELLQAFLDFRCS 446

Query: 444 VVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEFSLSEKQ 503
           VVERRARFKL QA+ER HIVEGI++GLDNLD VIN IR+ASSN++A+A+LR EF LSEKQ
Sbjct: 447 VVERRARFKLSQAQERNHIVEGIIVGLDNLDEVINTIRKASSNALAAASLRKEFELSEKQ 506

Query: 504 AEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIELKDKFP 563
           AEAILDI+LRRLT LER KF++E KSL   ISKL+ELLSS+K ILQLIE+EAIE+K+KF 
Sbjct: 507 AEAILDISLRRLTALERNKFVEEGKSLRTQISKLEELLSSKKQILQLIEEEAIEIKNKFF 566

Query: 564 SPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGKSVGKLR 623
           +PRRS+LEDTDSG LEDIDVIPNEEMLLA+SEKGYVKRMKP+TFNLQ+RGTIGKSVGKLR
Sbjct: 567 NPRRSMLEDTDSGDLEDIDVIPNEEMLLAISEKGYVKRMKPDTFNLQNRGTIGKSVGKLR 626

Query: 624 VNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSDGERITS 683
           VNDAMSDF+VCRAHD VLYFSDKGTVYS+ AYKIPEC+RTA GTPLVQILSLSDGERITS
Sbjct: 627 VNDAMSDFLVCRAHDKVLYFSDKGTVYSSPAYKIPECSRTAAGTPLVQILSLSDGERITS 686

Query: 684 IIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRRCANDDL 743
           IIPVSEF  DQ+L+MLT NGYIKKVSLN F+SIR TGIIAIQLV  DELKWV+ C+N+D 
Sbjct: 687 IIPVSEFAADQYLVMLTVNGYIKKVSLNYFASIRCTGIIAIQLVPDDELKWVKCCSNNDF 746

Query: 744 LAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLERMPTTP 803
           +AMASQNGMVIL+ C  IRA GRNTRG+VAMRLK GDK+ASMDIIP A++  L++     
Sbjct: 747 VAMASQNGMVILTPCANIRALGRNTRGSVAMRLKEGDKVASMDIIPDALQKELDKTLEVQ 806

Query: 804 R-----FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVFVVGFSL 863
           +       GPWLLFVSESG+GKRVP+S FR SPLNRVGL GYKFSSED LAAVFVVGFSL
Sbjct: 807 QRQYRSMKGPWLLFVSESGYGKRVPVSRFRTSPLNRVGLFGYKFSSEDCLAAVFVVGFSL 866

Query: 864 AEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLISAMDTE 906
            EDGESDEQVVLVSQSGTVNRIKVRD+SIQSRYARGVILMRL+HAGKIQSASLISA D +
Sbjct: 867 GEDGESDEQVVLVSQSGTVNRIKVRDISIQSRYARGVILMRLEHAGKIQSASLISAADAD 926

BLAST of MS001052 vs. ExPASy Swiss-Prot
Match: Q9CAF6 (DNA gyrase subunit A, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GYRA PE=1 SV=2)

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 686/926 (74.08%), Postives = 781/926 (84.34%), Query Frame = 0

Query: 1   MASSSGLRI-SSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSR--- 60
           M+ SS LR+ SSLLR  F       RFP +   L  LR    S++  F S    S +   
Sbjct: 19  MSLSSTLRLSSSLLRRSFF------RFP-LTDPLCRLRRTEPSATRFFSSRTPRSGKFVV 78

Query: 61  --------------GGEDGGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVR 120
                         G  +GG    G   R+V  ELHKEAT++YM+YA+SVLLGRALPDVR
Sbjct: 79  GAGKRGDEQVKEESGANNGGLVVSGDESRIVPFELHKEATESYMSYALSVLLGRALPDVR 138

Query: 121 DGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSL 180
           DGLKPVHRRILFAMHELG+SS+KP+KKCARVVGEVLGKFHPHGDTAVYDSLVRMAQ FSL
Sbjct: 139 DGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQSFSL 198

Query: 181 RCPLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPS 240
           RCPLIQGHGNFGSIDADP AAMRYTECRL+ L EA+LL+DL+ +TVDFV NFDNSQ+EP+
Sbjct: 199 RCPLIQGHGNFGSIDADPPAAMRYTECRLDPLAEAVLLSDLDQDTVDFVANFDNSQKEPA 258

Query: 241 LLPARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDF 300
           +LPARLP LLLNG+SGIAVGMATNIPPHNLGELVD LC LI NPEATLQELLEYMP PDF
Sbjct: 259 VLPARLPALLLNGASGIAVGMATNIPPHNLGELVDVLCALIHNPEATLQELLEYMPAPDF 318

Query: 301 PTGGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEK 360
           PTGG+IMGN G+L+AYRTGRGR+ VRGK +VELLD KTKR AVII E+PYQTNK+ LV+K
Sbjct: 319 PTGGIIMGNLGVLDAYRTGRGRVVVRGKAEVELLDPKTKRNAVIITEIPYQTNKATLVQK 378

Query: 361 IAELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVG 420
           IAELVENKTL+G+SDIRDESDR+GMR+VIELKRG DP ++ NNLYR T+LQS FSCNMVG
Sbjct: 379 IAELVENKTLEGISDIRDESDRNGMRVVIELKRGGDPALVLNNLYRHTALQSSFSCNMVG 438

Query: 421 IINGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINL 480
           I +G+PKLMGLKELLQAF+DFRCSVVERRARFKL  A++R+HI+EGIV+GLDN+D VI L
Sbjct: 439 ICDGEPKLMGLKELLQAFIDFRCSVVERRARFKLSHAQQRKHIIEGIVVGLDNVDEVIEL 498

Query: 481 IREASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDE 540
           I +ASS+S A+A L++E+ LSEKQAEAIL+I LRRLT LERKKF DES SL E I+KL++
Sbjct: 499 ITKASSHSSATAALQSEYGLSEKQAEAILEITLRRLTALERKKFTDESSSLTEQITKLEQ 558

Query: 541 LLSSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYV 600
           LLS+R NIL+LIEQEAIELKD+F SPRRS+LED+DSG LEDIDVIPNEEML+A+SEKGYV
Sbjct: 559 LLSTRTNILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMAVSEKGYV 618

Query: 601 KRMKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPE 660
           KRMK +TFNLQHRGTIGKSVGKLRV+DAMSDF+VC AHDHVL+FSD+G VYS RAYKIPE
Sbjct: 619 KRMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFFSDRGIVYSTRAYKIPE 678

Query: 661 CARTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRST 720
           C+R A GTPLVQILS+S+GER+TSI+PVSEF  D++LLMLT NG IKKVSL LFS IRST
Sbjct: 679 CSRNAAGTPLVQILSMSEGERVTSIVPVSEFAEDRYLLMLTVNGCIKKVSLKLFSGIRST 738

Query: 721 GIIAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAG 780
           GIIAIQL SGDELKWVR C++DDL+AMASQNGMV LS+CD +R   RNT+G  AMRLK  
Sbjct: 739 GIIAIQLNSGDELKWVRCCSSDDLVAMASQNGMVALSTCDGVRTLSRNTKGVTAMRLKNE 798

Query: 781 DKMASMDIIPAAVRNNLER----MPTTPRFNGPWLLFVSESGFGKRVPLSSFRLSPLNRV 840
           DK+ASMDIIPA++R ++E          +  GPWLLFV E+G+GKRVPLSSFR S LNRV
Sbjct: 799 DKIASMDIIPASLRKDMEEKSEDASLVKQSTGPWLLFVCENGYGKRVPLSSFRRSRLNRV 858

Query: 841 GLIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGV 900
           GL GYKF+ +DRLAAVFVVG+SLAEDGESDEQVVLVSQSGTVNRIKVRD+SIQSR ARGV
Sbjct: 859 GLSGYKFAEDDRLAAVFVVGYSLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRRARGV 918

Query: 901 ILMRLDHAGKIQSASLISAMDTEPEE 905
           ILMRLDHAGKIQSASLISA D E  E
Sbjct: 919 ILMRLDHAGKIQSASLISAADEEETE 937

BLAST of MS001052 vs. ExPASy Swiss-Prot
Match: Q7XZF7 (Probable DNA gyrase subunit A, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GYRA PE=2 SV=2)

HSP 1 Score: 1261.1 bits (3262), Expect = 0.0e+00
Identity = 643/876 (73.40%), Postives = 749/876 (85.50%), Query Frame = 0

Query: 36  LRFLPTSSSTSFRSIRLLSSRGGED----GGGGGDGGNGRVVHVELHKEATDAYMAYAMS 95
           +RFL +SSS+S  S  +   R G D     G GG     RVV VELHKEAT+AYMAYAMS
Sbjct: 42  VRFLSSSSSSSDGSRSVQPLRAGRDERAAAGEGGAAVKERVVPVELHKEATEAYMAYAMS 101

Query: 96  VLLGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYD 155
           VLLGRALPDVRDGLKPVHRRIL+AMHE+GL+SR+PF+KCARVVGEVLGKFHPHGD+AVY+
Sbjct: 102 VLLGRALPDVRDGLKPVHRRILYAMHEMGLASRRPFRKCARVVGEVLGKFHPHGDSAVYE 161

Query: 156 SLVRMAQDFSLRCPLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLAD--LELNTVD 215
           +LVRMAQDFS+R PL+QGHGNFGSIDADP AAMRYTECRL+      L  D    +  VD
Sbjct: 162 TLVRMAQDFSMRYPLVQGHGNFGSIDADPPAAMRYTECRLDVGCFFFLDIDAIFIIGQVD 221

Query: 216 FVPNFDNSQREPSLLPARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEAT 275
           FVPNFDNSQ+EPSLLPAR+P+LLLNGSSGIAVGMATNIPPHNLGELVD L V+I NPEAT
Sbjct: 222 FVPNFDNSQKEPSLLPARVPSLLLNGSSGIAVGMATNIPPHNLGELVDVLSVMIENPEAT 281

Query: 276 LQELLEYMPGPDFPTGGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKE 335
           LQELLE MPGPDFPTGG I+GN GILEAY+TGRGR+ +RGKTD+E +D+K+KR+A+IIKE
Sbjct: 282 LQELLECMPGPDFPTGGTIVGNQGILEAYKTGRGRVVMRGKTDIETIDVKSKRSAIIIKE 341

Query: 336 VPYQTNKSALVEKIAELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRL 395
           VPYQTNKS LVE+IAELVE K L+G+SDIRDESDRSGMR+VIELKRGADP I+ NNLYR 
Sbjct: 342 VPYQTNKSTLVERIAELVEEKVLEGISDIRDESDRSGMRVVIELKRGADPAIVLNNLYRH 401

Query: 396 TSLQSGFSCNMVGIINGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGI 455
           T+LQS FSCNMV I++GQPKLMGLKE+LQAF+DFRCSV+ERRARFKL QA ER+HIVEGI
Sbjct: 402 TALQSSFSCNMVAILDGQPKLMGLKEILQAFIDFRCSVIERRARFKLSQALERKHIVEGI 461

Query: 456 VIGLDNLDGVINLIREASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDE 515
           VIGLDNLD VI +IR  S++++A  +L  EF LS+KQAEA+LDI LR+LT LERKKF+DE
Sbjct: 462 VIGLDNLDSVIQIIRGTSNHAMARESLIKEFGLSDKQAEALLDITLRKLTSLERKKFVDE 521

Query: 516 SKSLMEHISKLDELLSSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPN 575
           +KSL E ISKL+ELLS++K I QLI QEA +LK+KF +PRRS +ED+ S +++D+D+IPN
Sbjct: 522 AKSLSEEISKLNELLSNKKLIFQLILQEATDLKNKFATPRRSFIEDSASTEVDDLDIIPN 581

Query: 576 EEMLLALSEKGYVKRMKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDK 635
           EEMLL LSEKGYVKRMKPNTFNLQ+RGTIGKSVGK+R+ND  SDFIVC+ HDHVLYFSDK
Sbjct: 582 EEMLLILSEKGYVKRMKPNTFNLQNRGTIGKSVGKMRMNDNTSDFIVCQTHDHVLYFSDK 641

Query: 636 GTVYSARAYKIPECARTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIK 695
           G VYSARAYKIPEC R ATGTPLVQ+LSLSDGERITSI+PV+EF  DQ+L+MLT NGYIK
Sbjct: 642 GIVYSARAYKIPECTRAATGTPLVQLLSLSDGERITSIVPVNEFGEDQYLVMLTVNGYIK 701

Query: 696 KVSLNLFSSIRSTGIIAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGR 755
           KV LN FS+IR++GII+IQL  GDELKWVRRC +DDL+A+ASQNGMVI+++C+ +RA GR
Sbjct: 702 KVPLNAFSAIRTSGIISIQLAPGDELKWVRRCGDDDLVALASQNGMVIVNTCNKLRALGR 761

Query: 756 NTRGAVAMRLKAGDKMASMDIIPAAVRNNLERMPTTPRFNGPWLLFVSESGFGKRVPLSS 815
            TRG +AM+LK GDKMASMDIIPA   N  E        + PWLLF++++G GKRVPL++
Sbjct: 762 KTRGVLAMKLKEGDKMASMDIIPATSHNMPETYSRVRDLSPPWLLFIADNGIGKRVPLNA 821

Query: 816 FRLSPLNRVGLIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVS 875
           FR    NRVGL GYK   +  LAAVFVVGFSL +DGESDEQVVLVSQSGTVNRIKV+D+S
Sbjct: 822 FRQGNFNRVGLQGYKLPPDCSLAAVFVVGFSLTDDGESDEQVVLVSQSGTVNRIKVKDIS 881

Query: 876 IQSRYARGVILMRLDHAGKIQSASLISAMDTEPEEE 906
           I+SR ARGVILMRL+HAGKIQSASLISA + E E++
Sbjct: 882 IRSRSARGVILMRLEHAGKIQSASLISAAEEEEEQD 917

BLAST of MS001052 vs. ExPASy Swiss-Prot
Match: Q55738 (DNA gyrase subunit A OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=gyrA PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 2.2e-240
Identity = 429/846 (50.71%), Postives = 590/846 (69.74%), Query Frame = 0

Query: 71  RVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKKC 130
           R++  +L  E + +Y+ YAMSV++GRALPD RDGLKPVHRRIL+AM+ELGL+  +PF+KC
Sbjct: 7   RLIATDLRNEMSQSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMYELGLTPDRPFRKC 66

Query: 131 ARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADPAAAMRYTECR 190
           ARVVGEVLGK+HPHGDTAVYD+LVRMAQDFS+R PLI GHGNFGS+D DP AAMRYTE R
Sbjct: 67  ARVVGEVLGKYHPHGDTAVYDALVRMAQDFSMREPLIDGHGNFGSVDNDPPAAMRYTESR 126

Query: 191 LEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIAVGMATNIPPH 250
           L  L+   LL D+E  TVDF+ NFD SQ+EP++LPAR+P LL+NGSSGIAVGMATNIPPH
Sbjct: 127 LRPLSTNSLLRDIEAETVDFIDNFDGSQQEPTVLPARIPQLLINGSSGIAVGMATNIPPH 186

Query: 251 NLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRTGRGRITVRGK 310
           NLGE++D    LIRNPE T QEL++ +PGPDFPTG  I+G +GI EAY TGRG IT+RG 
Sbjct: 187 NLGEVIDGAIALIRNPEITEQELMQIIPGPDFPTGAQILGRSGIREAYLTGRGSITMRGV 246

Query: 311 TDVELLDL--KTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRDESDRSGMR 370
             +E ++   +  R A+I+ E+PYQTNK+AL+E+IA+LV +K +DG++DIRDESDR GMR
Sbjct: 247 ASIETMEHPGRPDRDAIIVTELPYQTNKAALIERIADLVNDKKIDGIADIRDESDRDGMR 306

Query: 371 IVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAFLDFRCSVV 430
           IVIELKR A   ++ NNLY+ T +QS F  N++ ++NG P+++ +K+ L  F +FR   +
Sbjct: 307 IVIELKRDAYARVVLNNLYKQTPIQSNFGANLLALVNGTPEVLTIKKFLTVFWEFRIETI 366

Query: 431 ERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEFSLSEKQAE 490
            RR R++L++A+ER H+++G++I LDNLD VI LIR A+  + A   L   FSLSE QA+
Sbjct: 367 TRRTRYELRKAEERDHLLQGLLIALDNLDAVIRLIRGAADTASAKTELVEGFSLSEVQAD 426

Query: 491 AILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIELKDKFPSP 550
           AIL + LRRLT LE  K   E   L   I+   ++L+ R+ +  +IE+E  ++K    +P
Sbjct: 427 AILQMQLRRLTALEADKITAEHDELQTKIADFQDILARRERVNAIIEEELEQIKAIHATP 486

Query: 551 RRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGKSVGKLRVN 610
           RR+++   D G+L D D+I N++ L+ L+E+GY+KRM  +TF  Q+R T GK+  K++ +
Sbjct: 487 RRTVIVQED-GELIDTDLIANDQALILLTEQGYIKRMPASTFGTQNRATRGKAAAKIKDD 546

Query: 611 DAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSDGERITSII 670
           D +  F+ C  HD VL+FSD+G VYS  AY+IP  +RTA G P+VQ+L +   E+ITS++
Sbjct: 547 DGVEHFLSCCDHDKVLFFSDRGVVYSLNAYQIPIASRTARGVPIVQMLPIPKDEKITSLV 606

Query: 671 PVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRRCANDDLLA 730
            VSEFD D + +MLT  GYIKK +L+ FS+IR+ G+IAI LV GD+L+WVR    +D + 
Sbjct: 607 SVSEFDDDTYFIMLTKQGYIKKTALSAFSNIRANGLIAISLVEGDQLRWVRLAKAEDSVI 666

Query: 731 MASQNGMVI--LSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNL------- 790
           + SQ GM I   +  D +RA GR TRG  +MRL++GD + SMDI+P+ V  N+       
Sbjct: 667 IGSQKGMAIHFKADQDELRALGRATRGVKSMRLRSGDALISMDILPSQVVANIAVGSEDE 726

Query: 791 ---------ERMPTTPRFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKF-SSED 850
                    + +       GPWLL V+  GFGKRVP+  FRL     +G+   +F S +D
Sbjct: 727 PDEDLGGDTDAILEESDNPGPWLLGVTMKGFGKRVPIGQFRLQHRAGLGVKAIRFKSKDD 786

Query: 851 RLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKI 896
           +L A+ VV         +D+++++V+  G + R  V D+S QSR A GV + RLD    I
Sbjct: 787 QLVALHVV--------NADDELMIVTNRGIIIRQSVNDISPQSRSATGVRVQRLDADDAI 843

BLAST of MS001052 vs. ExPASy Swiss-Prot
Match: P94605 (DNA gyrase subunit A OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=gyrA PE=3 SV=2)

HSP 1 Score: 705.7 bits (1820), Expect = 6.9e-202
Identity = 372/837 (44.44%), Postives = 548/837 (65.47%), Query Frame = 0

Query: 70  GRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKK 129
           G+V+ V++  E    Y+ YAMSV++ RALPDVRDGLKPVHRRIL++MHELGL+  K ++K
Sbjct: 5   GKVLPVDISSEMKKCYIDYAMSVIVSRALPDVRDGLKPVHRRILYSMHELGLTPEKGYRK 64

Query: 130 CARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADPAAAMRYTEC 189
           CAR+VG+VLGK+HPHGD++VY +LVR+AQDF+LR  ++ GHGNFGS+D D AAAMRYTE 
Sbjct: 65  CARIVGDVLGKYHPHGDSSVYGALVRLAQDFNLRYTVVDGHGNFGSVDGDSAAAMRYTEA 124

Query: 190 RLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIAVGMATNIPP 249
           ++  +   M + D+  NTVDF+PNFD  ++EP +LP+R P LL+NGS+GIAVGMATNIPP
Sbjct: 125 KMNKIALEM-VRDIGKNTVDFIPNFDGEEKEPVVLPSRFPNLLVNGSAGIAVGMATNIPP 184

Query: 250 HNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRTGRGRITVRG 309
           HNLGE++D + +LI NPEAT+ EL+  + GPDFPT G+IMG +GI  AY TGRG+ITVR 
Sbjct: 185 HNLGEVIDGITMLIDNPEATILELMAQIKGPDFPTAGIIMGKSGIRAAYETGRGKITVRA 244

Query: 310 KTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRDESDRSGMRI 369
           K+++E+ D    +  +II E+PYQ NK+ LVE IA+LV++K + G+SD+RDESDR GMRI
Sbjct: 245 KSEIEVED--NGKQKIIITEIPYQVNKARLVESIADLVKDKRIVGISDLRDESDRDGMRI 304

Query: 370 VIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAFLDFRCSVVE 429
           VIE+K+ A+  II N LY+ T LQ  F  NM+ +++ +P+++ LK++LQ ++ F+  V+ 
Sbjct: 305 VIEIKKDANSNIILNQLYKHTRLQDTFGINMLALVDNRPEVLNLKQILQHYIKFQEQVIR 364

Query: 430 RRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEFSLSEKQAEA 489
           RR  F L++A  R HI+EG+ I LD++D VI+LIR + +   A   L  +F LSEKQA+A
Sbjct: 365 RRTEFDLEKASARAHILEGLKIALDHIDEVISLIRGSKTAQEAKLGLMDKFGLSEKQAQA 424

Query: 490 ILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIELKDKFPSPR 549
           ILD+ L+RLT LER+K  DE   LM+ I++L  +L+    IL +I  E  E+K K+   R
Sbjct: 425 ILDLKLQRLTGLEREKIEDEYNELMKTIAELKSILADENKILAIIRDELNEIKAKYGDER 484

Query: 550 RSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGKSVGKLRVND 609
           ++ +E  ++  ++  D+I  E +++ L+  GY+KR+  +T+  Q RG  G      + +D
Sbjct: 485 KTAIERAEN-DIDIEDLIQEENVVITLTHAGYIKRINADTYTSQKRGGRGIQAMTTKEDD 544

Query: 610 AMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSDGERITSIIP 669
            + +  +   H+++L+F++KG +Y  +AY+IPE  R A GT +V +L L   E+I ++I 
Sbjct: 545 FVENIFITSTHNNILFFTNKGRMYKLKAYQIPEAGRAAKGTNVVNLLQLDPNEKIQAVIS 604

Query: 670 VSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRRCANDDLLAM 729
           + EFD + FL+M T  G IKK  + ++ +IR +G+IAI L   DEL  VR    DD + +
Sbjct: 605 IKEFDEESFLVMCTKKGIIKKTVVGMYKNIRKSGLIAINLNDDDELVSVRITKGDDDIII 664

Query: 730 ASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLERMPTTPRFN 789
            +  G+ I  +   +R  GRN  G   + LK  D +  M++               P   
Sbjct: 665 VTNKGLAIRFNEVDVRPLGRNALGVKGITLKEDDFVVGMEV---------------PNQE 724

Query: 790 GPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVFVVGFSLAEDGESDE 849
              +L VSE+GFGKR  +  ++       GLI YK S +       +VG  + EDG   +
Sbjct: 725 SD-VLVVSENGFGKRTHVGEYKCQHRGGKGLITYKVSDKTGK----LVGVRMVEDG---D 784

Query: 850 QVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLISAMDTEPEEEA 907
           +++L++  G   RI V D+S  SR A GV LMR +   K+ + + I+  D+E  E++
Sbjct: 785 ELMLINNLGIAIRINVSDISTTSRNAMGVTLMRNNGDEKVLALAKINKDDSEQLEDS 814

BLAST of MS001052 vs. ExPASy TrEMBL
Match: A0A6J1BTL3 (DNA topoisomerase (ATP-hydrolyzing) OS=Momordica charantia OX=3673 GN=LOC111004692 PE=3 SV=1)

HSP 1 Score: 1742.6 bits (4512), Expect = 0.0e+00
Identity = 904/911 (99.23%), Postives = 905/911 (99.34%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSRGGED 60
           MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTS+STSFRSIRLLSSRGGE 
Sbjct: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSTSTSFRSIRLLSSRGGEV 60

Query: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120
           GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG
Sbjct: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120

Query: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180
           LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP
Sbjct: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180

Query: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240
           AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA
Sbjct: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240

Query: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300
           VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT
Sbjct: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300

Query: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360
           GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD
Sbjct: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360

Query: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420
           ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF
Sbjct: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420

Query: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480
           LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF
Sbjct: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480

Query: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540
           SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE
Sbjct: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540

Query: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600
           LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK
Sbjct: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600

Query: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660
           SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD
Sbjct: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660

Query: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720
           GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR
Sbjct: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720

Query: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780
           CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE
Sbjct: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780

Query: 781 RMPTTPR-----FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVF 840
           RMPTTPR     FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVF
Sbjct: 781 RMPTTPRSSSKGFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDRLAAVF 840

Query: 841 VVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLI 900
           VVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLI
Sbjct: 841 VVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQSASLI 900

Query: 901 SAMDTEPEEEA 907
           SAMDTEPEEEA
Sbjct: 901 SAMDTEPEEEA 911

BLAST of MS001052 vs. ExPASy TrEMBL
Match: A0A6J1BR81 (DNA topoisomerase (ATP-hydrolyzing) OS=Momordica charantia OX=3673 GN=LOC111004692 PE=3 SV=1)

HSP 1 Score: 1740.7 bits (4507), Expect = 0.0e+00
Identity = 904/916 (98.69%), Postives = 905/916 (98.80%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSRGGED 60
           MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTS+STSFRSIRLLSSRGGE 
Sbjct: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSTSTSFRSIRLLSSRGGEV 60

Query: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120
           GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG
Sbjct: 61  GGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDGLKPVHRRILFAMHELG 120

Query: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180
           LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP
Sbjct: 121 LSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRCPLIQGHGNFGSIDADP 180

Query: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240
           AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA
Sbjct: 181 AAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLLPARLPTLLLNGSSGIA 240

Query: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300
           VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT
Sbjct: 241 VGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPTGGLIMGNNGILEAYRT 300

Query: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360
           GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD
Sbjct: 301 GRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIAELVENKTLDGVSDIRD 360

Query: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420
           ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF
Sbjct: 361 ESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGIINGQPKLMGLKELLQAF 420

Query: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480
           LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF
Sbjct: 421 LDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIREASSNSIASANLRTEF 480

Query: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540
           SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE
Sbjct: 481 SLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELLSSRKNILQLIEQEAIE 540

Query: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600
           LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK
Sbjct: 541 LKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKRMKPNTFNLQHRGTIGK 600

Query: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660
           SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD
Sbjct: 601 SVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECARTATGTPLVQILSLSD 660

Query: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720
           GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR
Sbjct: 661 GERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGIIAIQLVSGDELKWVRR 720

Query: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780
           CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE
Sbjct: 721 CANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDKMASMDIIPAAVRNNLE 780

Query: 781 RMPTTPR----------FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDR 840
           RMPTTPR          FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDR
Sbjct: 781 RMPTTPRSSFGVSSSKGFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGYKFSSEDR 840

Query: 841 LAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQ 900
           LAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQ
Sbjct: 841 LAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRLDHAGKIQ 900

Query: 901 SASLISAMDTEPEEEA 907
           SASLISAMDTEPEEEA
Sbjct: 901 SASLISAMDTEPEEEA 916

BLAST of MS001052 vs. ExPASy TrEMBL
Match: A0A6J1HB61 (DNA topoisomerase (ATP-hydrolyzing) OS=Cucurbita moschata OX=3662 GN=LOC111462414 PE=3 SV=1)

HSP 1 Score: 1583.2 bits (4098), Expect = 0.0e+00
Identity = 824/927 (88.89%), Postives = 864/927 (93.20%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSR---- 60
           MA SSGLR SSLLRHQF PP    RF RI HGLSELRFL TS+S S RSIRL+ SR    
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHAWTRFGRIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  -------GGEDGGGG-----GDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDG 120
                  GG+DG G        G +GR+VH  LHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIVKDRGGSDGRIVHALLHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLL 240
           PLIQGHGNFGSIDADP AAMRYTECRLEALTEAMLL+DLEL+TVDFVPNFD+SQ+EPSLL
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELDTVDFVPNFDSSQKEPSLL 240

Query: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPT 300
           PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLI NPEATLQELLEYMPGPDFPT
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIA 360
           GGLIMGNNGILEAYRTGRGRITVRGKTD+ELLDLKTKRTAVII E+PYQTNKSALVEKIA
Sbjct: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDIELLDLKTKRTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGII 420
           ELVENKTLDG++DIRDESDRSGMRIV+ELKRGADPLIIQNNLYRLT+LQS FSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIR 480
           NGQPKLMGLKELLQAFLDFRCSVVERRARFKL QA+ERRHIVEGIV GLDNLD VI LIR
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRHIVEGIVTGLDNLDEVIQLIR 480

Query: 481 EASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELL 540
           EAS NS+ASANLR +FSLSEKQAEAILDINLRRLTHLERKKFIDESKSLME+ISKL+ELL
Sbjct: 481 EASRNSVASANLRAQFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMENISKLEELL 540

Query: 541 SSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKR 600
           SSRKNILQ+IEQEAIELK+KFPSPRRSIL+DTDSGQLEDIDVIPN+EMLLALSEKGYVKR
Sbjct: 541 SSRKNILQVIEQEAIELKNKFPSPRRSILKDTDSGQLEDIDVIPNDEMLLALSEKGYVKR 600

Query: 601 MKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECA 660
           MKP+TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYS+RAYKIPEC 
Sbjct: 601 MKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSRAYKIPECG 660

Query: 661 RTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGI 720
           RTATGTP++QILSLS+GERITSIIPVSEF  DQFLLMLTANGYIKKVSLN FSSIR+TGI
Sbjct: 661 RTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFFSSIRTTGI 720

Query: 721 IAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780
           IAIQLVSGDELKWVR C ND+L+AMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK
Sbjct: 721 IAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780

Query: 781 MASMDIIPAAVRNNLERMPTTPR-----FNGPWLLFVSESGFGKRVPLSSFRLSPLNRVG 840
           MASMDIIPA++ N+ ER+PTTPR      NGPWLLFVSESGFGKRVPLSSFRLSPL RVG
Sbjct: 781 MASMDIIPASMWNDSERIPTTPRSSAKGSNGPWLLFVSESGFGKRVPLSSFRLSPLKRVG 840

Query: 841 LIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVI 900
           LIGYKFS EDRLAAVFVVGF+LAEDGESDEQVVLVSQSGTVNRIKVRD+SIQSRYARGVI
Sbjct: 841 LIGYKFSLEDRLAAVFVVGFTLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRYARGVI 900

Query: 901 LMRLDHAGKIQSASLISAMDTEPEEEA 907
           LMRLD+AGKIQSASLISA DTEPEEEA
Sbjct: 901 LMRLDYAGKIQSASLISATDTEPEEEA 927

BLAST of MS001052 vs. ExPASy TrEMBL
Match: A0A1S3BDB8 (DNA topoisomerase (ATP-hydrolyzing) OS=Cucumis melo OX=3656 GN=LOC103488432 PE=3 SV=1)

HSP 1 Score: 1573.9 bits (4074), Expect = 0.0e+00
Identity = 821/922 (89.05%), Postives = 857/922 (92.95%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSS----- 60
           MASSSGLRIS LLRHQ  PP VSNRF R C GLSELRFLPT + T+ RS+RL  S     
Sbjct: 1   MASSSGLRISYLLRHQLAPPLVSNRFTRTCRGLSELRFLPTRNFTASRSLRLAKSGRRDE 60

Query: 61  ------RGGEDGGGG------GDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRD 120
                   G+DG G       G G +GR+VH  LHKEATDAYMAYAMSVLLGRALPDVRD
Sbjct: 61  LVKDEGEEGQDGNGSVAVKKDGGGSDGRIVHAALHKEATDAYMAYAMSVLLGRALPDVRD 120

Query: 121 GLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR 180
           GLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR
Sbjct: 121 GLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLR 180

Query: 181 CPLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSL 240
            PLIQGHGNFGS+DADP AAMRYTECRLEAL+EAMLL+DLE+NTVDFVPNFDNSQ+EPSL
Sbjct: 181 SPLIQGHGNFGSVDADPPAAMRYTECRLEALSEAMLLSDLEMNTVDFVPNFDNSQKEPSL 240

Query: 241 LPARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFP 300
           LPARLPTLLLNGSSGIAVGMATNIPPHNLGE+VDALCVLI NPEATLQELLEYMPGPDFP
Sbjct: 241 LPARLPTLLLNGSSGIAVGMATNIPPHNLGEVVDALCVLIHNPEATLQELLEYMPGPDFP 300

Query: 301 TGGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKI 360
           TGGLIMGNNGILEAYRTGRGRITVRGKT+VELLD KTKRTAVIIKE+PYQTNKSALVEKI
Sbjct: 301 TGGLIMGNNGILEAYRTGRGRITVRGKTEVELLDSKTKRTAVIIKEIPYQTNKSALVEKI 360

Query: 361 AELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGI 420
           AELVENKTLDG+SDIRDESDR+GMRIVIELKRGADP IIQNNLYRLTSLQS FSCNMVGI
Sbjct: 361 AELVENKTLDGISDIRDESDRTGMRIVIELKRGADPSIIQNNLYRLTSLQSSFSCNMVGI 420

Query: 421 INGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLI 480
           INGQPKLMGLKELLQAFLDFRCSVVERRARFKL QA+ERRHIVEGIVIGLDNLDGVI LI
Sbjct: 421 INGQPKLMGLKELLQAFLDFRCSVVERRARFKLSQAQERRHIVEGIVIGLDNLDGVIRLI 480

Query: 481 REASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDEL 540
           REASS+SIASA+LRT+F+LSEKQAEA+LDINLRRLTHLERKKF DESKSL E+ISKL+EL
Sbjct: 481 REASSHSIASASLRTQFNLSEKQAEAVLDINLRRLTHLERKKFTDESKSLTENISKLEEL 540

Query: 541 LSSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVK 600
           LSSRKNILQLIEQEA ELK+KFPSPRRS+LEDTDSGQLEDIDVIPNEEMLLALSEKGYVK
Sbjct: 541 LSSRKNILQLIEQEATELKNKFPSPRRSVLEDTDSGQLEDIDVIPNEEMLLALSEKGYVK 600

Query: 601 RMKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPEC 660
           RMKPNTF+LQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKG VYSARAYKIPEC
Sbjct: 601 RMKPNTFSLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGIVYSARAYKIPEC 660

Query: 661 ARTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTG 720
            RTA GTPLVQILSLSDGERITSIIPVSEF+ DQFLLMLTA GYIKKVSLN FSSIR+TG
Sbjct: 661 GRTAAGTPLVQILSLSDGERITSIIPVSEFNEDQFLLMLTAYGYIKKVSLNFFSSIRTTG 720

Query: 721 IIAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGD 780
           IIAIQLVSGDELKWVRRC ND+L+AMASQNGMVILSSCD +RA GRNTRGAVAMRLKAGD
Sbjct: 721 IIAIQLVSGDELKWVRRCTNDNLVAMASQNGMVILSSCDTVRALGRNTRGAVAMRLKAGD 780

Query: 781 KMASMDIIPAAVRNNLERMPTTPRFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVGLIGY 840
           KMASMDIIPAAV N+LER  ++   NGPWLLFVSESGFGKRVPLSSFRLSPL RVGLIGY
Sbjct: 781 KMASMDIIPAAVWNDLER-NSSKISNGPWLLFVSESGFGKRVPLSSFRLSPLRRVGLIGY 840

Query: 841 KFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVILMRL 900
           KFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSR+ARGVILMRL
Sbjct: 841 KFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRFARGVILMRL 900

Query: 901 DHAGKIQSASLISAMDTEPEEE 906
           DHAGKIQSASLISA DTEPEEE
Sbjct: 901 DHAGKIQSASLISAADTEPEEE 921

BLAST of MS001052 vs. ExPASy TrEMBL
Match: A0A6J1K6F5 (DNA topoisomerase (ATP-hydrolyzing) OS=Cucurbita maxima OX=3661 GN=LOC111492096 PE=3 SV=1)

HSP 1 Score: 1563.9 bits (4048), Expect = 0.0e+00
Identity = 817/927 (88.13%), Postives = 858/927 (92.56%), Query Frame = 0

Query: 1   MASSSGLRISSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSR---- 60
           MA SSGLR SSLLRHQF PP  S+RF RI HGLSELRFL TS+S S RSIRL+ SR    
Sbjct: 1   MAFSSGLRFSSLLRHQFAPPHASSRFARIYHGLSELRFLSTSTSKSSRSIRLVKSRRKDE 60

Query: 61  -------GGEDGGGG-----GDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVRDG 120
                  GG+DG G        G +GR+V  ELHKEATDAYMAYAMSVLLGRALPDVRDG
Sbjct: 61  SVKEEGVGGQDGNGSVIVKDRGGSDGRIVQAELHKEATDAYMAYAMSVLLGRALPDVRDG 120

Query: 121 LKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180
           LKPVHRR+LFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC
Sbjct: 121 LKPVHRRVLFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSLRC 180

Query: 181 PLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPSLL 240
           PLIQGHGNFGSIDADP AAMRYTECRLEALTEAMLL+DLELNTVDFVPNFD+SQ+EPSLL
Sbjct: 181 PLIQGHGNFGSIDADPPAAMRYTECRLEALTEAMLLSDLELNTVDFVPNFDSSQKEPSLL 240

Query: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDFPT 300
           PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLI NPEATLQELLEYMPGPDFPT
Sbjct: 241 PARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIHNPEATLQELLEYMPGPDFPT 300

Query: 301 GGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEKIA 360
           GGLIMGNNGILEAYRTGRGRI VRGKTDVELLDLKTK+TAVII E+PYQTNKSALVEKIA
Sbjct: 301 GGLIMGNNGILEAYRTGRGRIIVRGKTDVELLDLKTKQTAVIINEIPYQTNKSALVEKIA 360

Query: 361 ELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVGII 420
           ELVENKTLDG++DIRDESDRSGMRIV+ELKRGADPLIIQNNLYRLT+LQS FSCNMVGII
Sbjct: 361 ELVENKTLDGIADIRDESDRSGMRIVVELKRGADPLIIQNNLYRLTALQSSFSCNMVGII 420

Query: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINLIR 480
           NGQPKLMGLKELLQAFLDFRCSVVERRARFKL QA+ERRHIVEGIV GLDNLD VI LIR
Sbjct: 421 NGQPKLMGLKELLQAFLDFRCSVVERRARFKLLQAQERRHIVEGIVTGLDNLDEVIQLIR 480

Query: 481 EASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDELL 540
           EAS  S+ASA LR +FSLSEKQAEA+LDINL RL+HLERKKF DESKSLM++ISKL+ELL
Sbjct: 481 EASRTSVASAYLRAQFSLSEKQAEALLDINLGRLSHLERKKFTDESKSLMKNISKLEELL 540

Query: 541 SSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYVKR 600
           SSRKNILQLIEQEAIELK+KF SPRRSIL+DTDSGQLEDIDVIPNEEMLLALSEKGYVKR
Sbjct: 541 SSRKNILQLIEQEAIELKNKFSSPRRSILKDTDSGQLEDIDVIPNEEMLLALSEKGYVKR 600

Query: 601 MKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPECA 660
           MKP+TFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYS+RAYKIPEC 
Sbjct: 601 MKPSTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSSRAYKIPECG 660

Query: 661 RTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRSTGI 720
           RTATGTP++QILSLS+GERITSIIPVSEF  DQFLLMLTANGYIKKVSLN FSSIR+TGI
Sbjct: 661 RTATGTPIIQILSLSEGERITSIIPVSEFVEDQFLLMLTANGYIKKVSLNFFSSIRTTGI 720

Query: 721 IAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780
           IAIQLVSGDELKWVR C ND+L+AMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK
Sbjct: 721 IAIQLVSGDELKWVRCCTNDNLVAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAGDK 780

Query: 781 MASMDIIPAAVRNNLERMPTTP-----RFNGPWLLFVSESGFGKRVPLSSFRLSPLNRVG 840
           MASMDIIPA+V N+ ER+PTTP       NGPWLLFVSESGFGKRVPLSSFRL PLNRVG
Sbjct: 781 MASMDIIPASVWNDSERIPTTPLSSSKGSNGPWLLFVSESGFGKRVPLSSFRLFPLNRVG 840

Query: 841 LIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGVI 900
           LIGYKFS EDRLAAVFVVGF+LAEDGESDEQVVLVSQSGTVNRIKVRD+SIQSRYARGVI
Sbjct: 841 LIGYKFSLEDRLAAVFVVGFTLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRYARGVI 900

Query: 901 LMRLDHAGKIQSASLISAMDTEPEEEA 907
           LM+L +AGKIQSASLISA DTEPEEEA
Sbjct: 901 LMKLVNAGKIQSASLISATDTEPEEEA 927

BLAST of MS001052 vs. TAIR 10
Match: AT3G10690.1 (DNA GYRASE A )

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 686/926 (74.08%), Postives = 781/926 (84.34%), Query Frame = 0

Query: 1   MASSSGLRI-SSLLRHQFVPPSVSNRFPRICHGLSELRFLPTSSSTSFRSIRLLSSR--- 60
           M+ SS LR+ SSLLR  F       RFP +   L  LR    S++  F S    S +   
Sbjct: 19  MSLSSTLRLSSSLLRRSFF------RFP-LTDPLCRLRRTEPSATRFFSSRTPRSGKFVV 78

Query: 61  --------------GGEDGGGGGDGGNGRVVHVELHKEATDAYMAYAMSVLLGRALPDVR 120
                         G  +GG    G   R+V  ELHKEAT++YM+YA+SVLLGRALPDVR
Sbjct: 79  GAGKRGDEQVKEESGANNGGLVVSGDESRIVPFELHKEATESYMSYALSVLLGRALPDVR 138

Query: 121 DGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQDFSL 180
           DGLKPVHRRILFAMHELG+SS+KP+KKCARVVGEVLGKFHPHGDTAVYDSLVRMAQ FSL
Sbjct: 139 DGLKPVHRRILFAMHELGMSSKKPYKKCARVVGEVLGKFHPHGDTAVYDSLVRMAQSFSL 198

Query: 181 RCPLIQGHGNFGSIDADPAAAMRYTECRLEALTEAMLLADLELNTVDFVPNFDNSQREPS 240
           RCPLIQGHGNFGSIDADP AAMRYTECRL+ L EA+LL+DL+ +TVDFV NFDNSQ+EP+
Sbjct: 199 RCPLIQGHGNFGSIDADPPAAMRYTECRLDPLAEAVLLSDLDQDTVDFVANFDNSQKEPA 258

Query: 241 LLPARLPTLLLNGSSGIAVGMATNIPPHNLGELVDALCVLIRNPEATLQELLEYMPGPDF 300
           +LPARLP LLLNG+SGIAVGMATNIPPHNLGELVD LC LI NPEATLQELLEYMP PDF
Sbjct: 259 VLPARLPALLLNGASGIAVGMATNIPPHNLGELVDVLCALIHNPEATLQELLEYMPAPDF 318

Query: 301 PTGGLIMGNNGILEAYRTGRGRITVRGKTDVELLDLKTKRTAVIIKEVPYQTNKSALVEK 360
           PTGG+IMGN G+L+AYRTGRGR+ VRGK +VELLD KTKR AVII E+PYQTNK+ LV+K
Sbjct: 319 PTGGIIMGNLGVLDAYRTGRGRVVVRGKAEVELLDPKTKRNAVIITEIPYQTNKATLVQK 378

Query: 361 IAELVENKTLDGVSDIRDESDRSGMRIVIELKRGADPLIIQNNLYRLTSLQSGFSCNMVG 420
           IAELVENKTL+G+SDIRDESDR+GMR+VIELKRG DP ++ NNLYR T+LQS FSCNMVG
Sbjct: 379 IAELVENKTLEGISDIRDESDRNGMRVVIELKRGGDPALVLNNLYRHTALQSSFSCNMVG 438

Query: 421 IINGQPKLMGLKELLQAFLDFRCSVVERRARFKLKQAKERRHIVEGIVIGLDNLDGVINL 480
           I +G+PKLMGLKELLQAF+DFRCSVVERRARFKL  A++R+HI+EGIV+GLDN+D VI L
Sbjct: 439 ICDGEPKLMGLKELLQAFIDFRCSVVERRARFKLSHAQQRKHIIEGIVVGLDNVDEVIEL 498

Query: 481 IREASSNSIASANLRTEFSLSEKQAEAILDINLRRLTHLERKKFIDESKSLMEHISKLDE 540
           I +ASS+S A+A L++E+ LSEKQAEAIL+I LRRLT LERKKF DES SL E I+KL++
Sbjct: 499 ITKASSHSSATAALQSEYGLSEKQAEAILEITLRRLTALERKKFTDESSSLTEQITKLEQ 558

Query: 541 LLSSRKNILQLIEQEAIELKDKFPSPRRSILEDTDSGQLEDIDVIPNEEMLLALSEKGYV 600
           LLS+R NIL+LIEQEAIELKD+F SPRRS+LED+DSG LEDIDVIPNEEML+A+SEKGYV
Sbjct: 559 LLSTRTNILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMAVSEKGYV 618

Query: 601 KRMKPNTFNLQHRGTIGKSVGKLRVNDAMSDFIVCRAHDHVLYFSDKGTVYSARAYKIPE 660
           KRMK +TFNLQHRGTIGKSVGKLRV+DAMSDF+VC AHDHVL+FSD+G VYS RAYKIPE
Sbjct: 619 KRMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFFSDRGIVYSTRAYKIPE 678

Query: 661 CARTATGTPLVQILSLSDGERITSIIPVSEFDGDQFLLMLTANGYIKKVSLNLFSSIRST 720
           C+R A GTPLVQILS+S+GER+TSI+PVSEF  D++LLMLT NG IKKVSL LFS IRST
Sbjct: 679 CSRNAAGTPLVQILSMSEGERVTSIVPVSEFAEDRYLLMLTVNGCIKKVSLKLFSGIRST 738

Query: 721 GIIAIQLVSGDELKWVRRCANDDLLAMASQNGMVILSSCDIIRAQGRNTRGAVAMRLKAG 780
           GIIAIQL SGDELKWVR C++DDL+AMASQNGMV LS+CD +R   RNT+G  AMRLK  
Sbjct: 739 GIIAIQLNSGDELKWVRCCSSDDLVAMASQNGMVALSTCDGVRTLSRNTKGVTAMRLKNE 798

Query: 781 DKMASMDIIPAAVRNNLER----MPTTPRFNGPWLLFVSESGFGKRVPLSSFRLSPLNRV 840
           DK+ASMDIIPA++R ++E          +  GPWLLFV E+G+GKRVPLSSFR S LNRV
Sbjct: 799 DKIASMDIIPASLRKDMEEKSEDASLVKQSTGPWLLFVCENGYGKRVPLSSFRRSRLNRV 858

Query: 841 GLIGYKFSSEDRLAAVFVVGFSLAEDGESDEQVVLVSQSGTVNRIKVRDVSIQSRYARGV 900
           GL GYKF+ +DRLAAVFVVG+SLAEDGESDEQVVLVSQSGTVNRIKVRD+SIQSR ARGV
Sbjct: 859 GLSGYKFAEDDRLAAVFVVGYSLAEDGESDEQVVLVSQSGTVNRIKVRDISIQSRRARGV 918

Query: 901 ILMRLDHAGKIQSASLISAMDTEPEE 905
           ILMRLDHAGKIQSASLISA D E  E
Sbjct: 919 ILMRLDHAGKIQSASLISAADEEETE 937

BLAST of MS001052 vs. TAIR 10
Match: AT3G23890.1 (topoisomerase II )

HSP 1 Score: 79.0 bits (193), Expect = 2.2e-14
Identity = 58/169 (34.32%), Postives = 95/169 (56.21%), Query Frame = 0

Query: 94  LGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEV--LGKFHPHGDTAVYD 153
           L R++P + DGLKP  R+ILF   +    +RK   K A++VG V  L  +H HG+ ++  
Sbjct: 700 LQRSIPSMVDGLKPGQRKILFVAFK--KIARKEM-KVAQLVGYVSLLSAYH-HGEQSLAS 759

Query: 154 SLVRMAQDF--SLRCPLIQGHGNFG--SIDADPAAAMRYTECRLEALTEAMLLADLELNT 213
           +++ MAQD+  S    L+  +G FG  +     +A+ RY   +L  +T  +   D +L  
Sbjct: 760 AIIGMAQDYVGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDL-L 819

Query: 214 VDFVPNFDNSQREPSLLPARLPTLLLNGSSGIAVGMATNIPPHNLGELV 257
           +D++ N D  + EP+     +PT+L+NG+ GI  G +T IP +N  E+V
Sbjct: 820 LDYL-NEDGQRIEPTWYMPIIPTVLVNGAEGIGTGWSTFIPNYNPREIV 862

BLAST of MS001052 vs. TAIR 10
Match: AT3G23890.2 (topoisomerase II )

HSP 1 Score: 79.0 bits (193), Expect = 2.2e-14
Identity = 58/169 (34.32%), Postives = 95/169 (56.21%), Query Frame = 0

Query: 94  LGRALPDVRDGLKPVHRRILFAMHELGLSSRKPFKKCARVVGEV--LGKFHPHGDTAVYD 153
           L R++P + DGLKP  R+ILF   +    +RK   K A++VG V  L  +H HG+ ++  
Sbjct: 700 LQRSIPSMVDGLKPGQRKILFVAFK--KIARKEM-KVAQLVGYVSLLSAYH-HGEQSLAS 759

Query: 154 SLVRMAQDF--SLRCPLIQGHGNFG--SIDADPAAAMRYTECRLEALTEAMLLADLELNT 213
           +++ MAQD+  S    L+  +G FG  +     +A+ RY   +L  +T  +   D +L  
Sbjct: 760 AIIGMAQDYVGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDL-L 819

Query: 214 VDFVPNFDNSQREPSLLPARLPTLLLNGSSGIAVGMATNIPPHNLGELV 257
           +D++ N D  + EP+     +PT+L+NG+ GI  G +T IP +N  E+V
Sbjct: 820 LDYL-NEDGQRIEPTWYMPIIPTVLVNGAEGIGTGWSTFIPNYNPREIV 862