MS001027 (gene) Bitter gourd (TR) v1

BLAST of MS001027 vs. NCBI nr
Match: XP_022131619.1 (uncharacterized protein LOC111004748 [Momordica charantia])

HSP 1 Score: 747.7 bits (1929), Expect = 5.2e-212
Identity = 387/391 (98.98%), Postives = 387/391 (98.98%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD
Sbjct: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS
Sbjct: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKA ASHGDDSDTNFDV
Sbjct: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKATASHGDDSDTNFDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQMKQQCEVQVCP PQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE
Sbjct: 181 ENQMKQQCEVQVCPSPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NINLKEQLRVDVRKELS LEMKCTDMASLLRGLGIHVGSGLHPSSQEVQ AYKRALLKFH
Sbjct: 301 NINLKEQLRVDVRKELSFLEMKCTDMASLLRGLGIHVGSGLHPSSQEVQVAYKRALLKFH 360

Query: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKKFMC 392
           PDRVSSTDIRQLVEAEEKFKLISHMKKKFMC
Sbjct: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKKFMC 391

BLAST of MS001027 vs. NCBI nr
Match: XP_038886123.1 (uncharacterized protein LOC120076385 [Benincasa hispida] >XP_038886124.1 uncharacterized protein LOC120076385 [Benincasa hispida] >XP_038886125.1 uncharacterized protein LOC120076385 [Benincasa hispida])

HSP 1 Score: 606.7 bits (1563), Expect = 1.4e-169
Identity = 319/393 (81.17%), Postives = 346/393 (88.04%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MA+GSHENVAFIDV++YRL+DVIIIDDTEC+ K+FQ  NV+TE R+SP  IYVD CSSD+
Sbjct: 1   MAEGSHENVAFIDVENYRLNDVIIIDDTECMTKKFQRPNVSTESRRSPSCIYVDDCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFP SS RHFVE+DGRQS+K YAF MGKC + NYGKA ARN FGLH  SHSSS
Sbjct: 61  DACSPSRTFPPSSRRHFVEVDGRQSQKQYAFFMGKCMRSNYGKAPARNHFGLHTSSHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+S+SSDCEVMEGSFEKL+KQWEEASLRRKHD R AYFTVD++DKA ASHG DSDTNFDV
Sbjct: 121 SDSESSDCEVMEGSFEKLRKQWEEASLRRKHDVRRAYFTVDEQDKASASHGGDSDTNFDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPI--TSFDVKDIICERERLKET 240
           ENQMKQQ EVQ C  P+   N    LLTE+K  S E  P+      VKD++CERERLKET
Sbjct: 181 ENQMKQQSEVQACFSPENVENGSCCLLTELKSNSTENGPLARAPLTVKDLVCERERLKET 240

Query: 241 DEYRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKD 300
           DEYRRAMEEEWTTRQQ+LQIQAEEAQRLRRKRKA+RMRLLDMQRRQMKRLEEVRETQKKD
Sbjct: 241 DEYRRAMEEEWTTRQQELQIQAEEAQRLRRKRKAERMRLLDMQRRQMKRLEEVRETQKKD 300

Query: 301 EENINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLK 360
           EENINLKE+LRVDVRKELS LEM CTDMASLLRGLGI +GSGL PSSQEVQAAYKRALLK
Sbjct: 301 EENINLKERLRVDVRKELSKLEMNCTDMASLLRGLGIQIGSGLQPSSQEVQAAYKRALLK 360

Query: 361 FHPDRVSSTDIRQLVEAEEKFKLISHMKKKFMC 392
           FHPDRVSSTDIRQLVEAEEKFKLISHMKK+FMC
Sbjct: 361 FHPDRVSSTDIRQLVEAEEKFKLISHMKKRFMC 393

BLAST of MS001027 vs. NCBI nr
Match: XP_004144161.2 (uncharacterized protein LOC101218935 [Cucumis sativus] >XP_031742597.1 uncharacterized protein LOC101218935 [Cucumis sativus] >KGN47666.1 hypothetical protein Csa_018231 [Cucumis sativus])

HSP 1 Score: 602.4 bits (1552), Expect = 2.7e-168
Identity = 318/393 (80.92%), Postives = 342/393 (87.02%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVAFIDV++YRLDDVIIIDDT+CV K+FQ +N++TE RKSP  IYVD CSSD+
Sbjct: 1   MAKGSHENVAFIDVENYRLDDVIIIDDTQCVTKDFQRANISTESRKSPSCIYVDDCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFP SS RHFVELDGRQSRK YAF MGKC Q N GKA ARN FGLH+ +HSSS
Sbjct: 61  DACSPSRTFPPSSRRHFVELDGRQSRKQYAFFMGKCMQSNCGKAPARNHFGLHVSAHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+SDSSDCEVMEGSFEKL++QWEEASLRRKHD R A+FTV+D+DKA ASHG DSDTN  V
Sbjct: 121 SDSDSSDCEVMEGSFEKLRQQWEEASLRRKHDDRRAFFTVEDQDKASASHGGDSDTNVGV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQ KQQ EVQVC  P+   N G  LLTE+K  S E  P      K +ICERERLKETDE
Sbjct: 181 ENQRKQQSEVQVCSSPENAENGGCCLLTELKSNSTENGPPVPLSAKGLICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQ+LQIQAEEA +LRRKRKA+RMRLLDMQRRQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQELQIQAEEAHKLRRKRKAERMRLLDMQRRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NIN+KEQLR DVRKELS+LEM CTDMASLLRGLGI VGSGL PSSQEVQAAYKRALLKFH
Sbjct: 301 NINMKEQLRADVRKELSMLEMNCTDMASLLRGLGIQVGSGLQPSSQEVQAAYKRALLKFH 360

Query: 361 PDRVS--STDIRQLVEAEEKFKLISHMKKKFMC 392
           PDRVS  STDIRQLVEAEEKFKLISHMKKKFMC
Sbjct: 361 PDRVSSTSTDIRQLVEAEEKFKLISHMKKKFMC 393

BLAST of MS001027 vs. NCBI nr
Match: XP_008445431.1 (PREDICTED: uncharacterized protein LOC103488453 [Cucumis melo] >XP_008445432.1 PREDICTED: uncharacterized protein LOC103488453 [Cucumis melo])

HSP 1 Score: 601.3 bits (1549), Expect = 6.0e-168
Identity = 316/393 (80.41%), Postives = 342/393 (87.02%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVA+IDV++YRLDDVIIIDDT+CV K FQ ++++TE RKSP  IYVD CSSD+
Sbjct: 1   MAKGSHENVAYIDVENYRLDDVIIIDDTQCVTKNFQRADISTESRKSPSCIYVDDCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTF  SS RHFVELDGRQ+RK YAF MGKC Q NYGKA ARN F LH+ +HSSS
Sbjct: 61  DACSPSRTFRPSSRRHFVELDGRQNRKQYAFFMGKCMQSNYGKAPARNHFDLHVSAHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+SDSSDCEVMEGSFEKL++QWEEASLRRKHD R  +FTV+D+DKA ASHG DSDTNFDV
Sbjct: 121 SDSDSSDCEVMEGSFEKLRQQWEEASLRRKHDDRRTFFTVEDQDKASASHGGDSDTNFDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQMKQQ EVQ C  P+   N G  LLTE+K  SAE  P      KD+ICERERLKETDE
Sbjct: 181 ENQMKQQSEVQACSSPENAENGGCCLLTELKSNSAENGPPAPLSTKDLICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQ+LQIQAEEA +LRRKRKA+RMRLLDMQRRQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQELQIQAEEAHKLRRKRKAERMRLLDMQRRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NIN+KEQLR DVRKELS+LEM CTDMASLLRGLGI VGS L PSSQEVQAAYKRALLKFH
Sbjct: 301 NINMKEQLRADVRKELSMLEMNCTDMASLLRGLGIQVGSSLQPSSQEVQAAYKRALLKFH 360

Query: 361 PDRVS--STDIRQLVEAEEKFKLISHMKKKFMC 392
           PDRVS  STDIRQLVEAEEKFKLISHMKKKFMC
Sbjct: 361 PDRVSSTSTDIRQLVEAEEKFKLISHMKKKFMC 393

BLAST of MS001027 vs. NCBI nr
Match: XP_023546909.1 (uncharacterized protein LOC111805871 [Cucurbita pepo subsp. pepo] >XP_023546910.1 uncharacterized protein LOC111805871 [Cucurbita pepo subsp. pepo] >XP_023546911.1 uncharacterized protein LOC111805871 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 600.9 bits (1548), Expect = 7.9e-168
Identity = 322/390 (82.56%), Postives = 340/390 (87.18%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVAFIDV++YRLDDVIIIDDTE V K+FQ   V+ + RKSP  IYVDGCSSD+
Sbjct: 1   MAKGSHENVAFIDVENYRLDDVIIIDDTEFVTKKFQRPKVSAKSRKSPPCIYVDGCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFPSSS RHFVELDGRQSRK YAF MGKCR+ N GKA  RN FGLHI SHSSS
Sbjct: 61  DACSPSRTFPSSSQRHFVELDGRQSRKQYAFFMGKCRRGNCGKAPVRNHFGLHISSHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+SDSSDCEVMEGSFE L+KQWEEASLRRKHD R AYF  DD+DKA ASH  DSDTNFDV
Sbjct: 121 SDSDSSDCEVMEGSFENLRKQWEEASLRRKHDVRRAYFRADDQDKASASHRVDSDTNFDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQMKQ+ +VQ C   +   N G  LLT  K  SAE   +T F VKD+ICERERLKETDE
Sbjct: 181 ENQMKQKSDVQACSSSKNAENGGCCLLTGPKSNSAENGSLTPFAVKDLICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQ+LQIQAEEAQRLRRKRKADRMRLLDMQ+RQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQELQIQAEEAQRLRRKRKADRMRLLDMQKRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NINLKEQLRV+VRKELS LEM CTDMASLLRGLGI VGSGLHPSSQEVQAAYKRALLKFH
Sbjct: 301 NINLKEQLRVNVRKELSKLEMNCTDMASLLRGLGIQVGSGLHPSSQEVQAAYKRALLKFH 360

Query: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKKFM 391
           PDRVSSTDIRQLVEAEEKFKLISHMKK FM
Sbjct: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKTFM 390

BLAST of MS001027 vs. ExPASy TrEMBL
Match: A0A6J1BQR9 (uncharacterized protein LOC111004748 OS=Momordica charantia OX=3673 GN=LOC111004748 PE=4 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 2.5e-212
Identity = 387/391 (98.98%), Postives = 387/391 (98.98%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD
Sbjct: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS
Sbjct: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKA ASHGDDSDTNFDV
Sbjct: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKATASHGDDSDTNFDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQMKQQCEVQVCP PQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE
Sbjct: 181 ENQMKQQCEVQVCPSPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NINLKEQLRVDVRKELS LEMKCTDMASLLRGLGIHVGSGLHPSSQEVQ AYKRALLKFH
Sbjct: 301 NINLKEQLRVDVRKELSFLEMKCTDMASLLRGLGIHVGSGLHPSSQEVQVAYKRALLKFH 360

Query: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKKFMC 392
           PDRVSSTDIRQLVEAEEKFKLISHMKKKFMC
Sbjct: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKKFMC 391

BLAST of MS001027 vs. ExPASy TrEMBL
Match: A0A0A0KIS0 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G367120 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.3e-168
Identity = 318/393 (80.92%), Postives = 342/393 (87.02%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVAFIDV++YRLDDVIIIDDT+CV K+FQ +N++TE RKSP  IYVD CSSD+
Sbjct: 1   MAKGSHENVAFIDVENYRLDDVIIIDDTQCVTKDFQRANISTESRKSPSCIYVDDCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFP SS RHFVELDGRQSRK YAF MGKC Q N GKA ARN FGLH+ +HSSS
Sbjct: 61  DACSPSRTFPPSSRRHFVELDGRQSRKQYAFFMGKCMQSNCGKAPARNHFGLHVSAHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+SDSSDCEVMEGSFEKL++QWEEASLRRKHD R A+FTV+D+DKA ASHG DSDTN  V
Sbjct: 121 SDSDSSDCEVMEGSFEKLRQQWEEASLRRKHDDRRAFFTVEDQDKASASHGGDSDTNVGV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQ KQQ EVQVC  P+   N G  LLTE+K  S E  P      K +ICERERLKETDE
Sbjct: 181 ENQRKQQSEVQVCSSPENAENGGCCLLTELKSNSTENGPPVPLSAKGLICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQ+LQIQAEEA +LRRKRKA+RMRLLDMQRRQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQELQIQAEEAHKLRRKRKAERMRLLDMQRRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NIN+KEQLR DVRKELS+LEM CTDMASLLRGLGI VGSGL PSSQEVQAAYKRALLKFH
Sbjct: 301 NINMKEQLRADVRKELSMLEMNCTDMASLLRGLGIQVGSGLQPSSQEVQAAYKRALLKFH 360

Query: 361 PDRVS--STDIRQLVEAEEKFKLISHMKKKFMC 392
           PDRVS  STDIRQLVEAEEKFKLISHMKKKFMC
Sbjct: 361 PDRVSSTSTDIRQLVEAEEKFKLISHMKKKFMC 393

BLAST of MS001027 vs. ExPASy TrEMBL
Match: A0A1S3BCQ4 (uncharacterized protein LOC103488453 OS=Cucumis melo OX=3656 GN=LOC103488453 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.9e-168
Identity = 316/393 (80.41%), Postives = 342/393 (87.02%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVA+IDV++YRLDDVIIIDDT+CV K FQ ++++TE RKSP  IYVD CSSD+
Sbjct: 1   MAKGSHENVAYIDVENYRLDDVIIIDDTQCVTKNFQRADISTESRKSPSCIYVDDCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTF  SS RHFVELDGRQ+RK YAF MGKC Q NYGKA ARN F LH+ +HSSS
Sbjct: 61  DACSPSRTFRPSSRRHFVELDGRQNRKQYAFFMGKCMQSNYGKAPARNHFDLHVSAHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+SDSSDCEVMEGSFEKL++QWEEASLRRKHD R  +FTV+D+DKA ASHG DSDTNFDV
Sbjct: 121 SDSDSSDCEVMEGSFEKLRQQWEEASLRRKHDDRRTFFTVEDQDKASASHGGDSDTNFDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQMKQQ EVQ C  P+   N G  LLTE+K  SAE  P      KD+ICERERLKETDE
Sbjct: 181 ENQMKQQSEVQACSSPENAENGGCCLLTELKSNSAENGPPAPLSTKDLICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQ+LQIQAEEA +LRRKRKA+RMRLLDMQRRQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQELQIQAEEAHKLRRKRKAERMRLLDMQRRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NIN+KEQLR DVRKELS+LEM CTDMASLLRGLGI VGS L PSSQEVQAAYKRALLKFH
Sbjct: 301 NINMKEQLRADVRKELSMLEMNCTDMASLLRGLGIQVGSSLQPSSQEVQAAYKRALLKFH 360

Query: 361 PDRVS--STDIRQLVEAEEKFKLISHMKKKFMC 392
           PDRVS  STDIRQLVEAEEKFKLISHMKKKFMC
Sbjct: 361 PDRVSSTSTDIRQLVEAEEKFKLISHMKKKFMC 393

BLAST of MS001027 vs. ExPASy TrEMBL
Match: A0A6J1K4A4 (uncharacterized protein LOC111492154 OS=Cucurbita maxima OX=3661 GN=LOC111492154 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.5e-167
Identity = 322/390 (82.56%), Postives = 339/390 (86.92%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVAFIDV++YRLDDVIIIDDTE V K+FQ   V+ + RKSP  IYVDGCSSD+
Sbjct: 1   MAKGSHENVAFIDVENYRLDDVIIIDDTEFVTKKFQRPKVSAKSRKSPPCIYVDGCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFPSSS RHFVELDGRQSRK YAF MGKCR+ N GKA  RN FGLHI SHSSS
Sbjct: 61  DACSPSRTFPSSSQRHFVELDGRQSRKQYAFFMGKCRRGNCGKAPVRNHFGLHISSHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+SDSSDCEVMEGSFE L+KQWEEASLRRKHD R AYF VDD+DKA ASH  DSDTNFDV
Sbjct: 121 SDSDSSDCEVMEGSFENLRKQWEEASLRRKHDVRRAYFRVDDQDKASASHRVDSDTNFDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQMKQ+ EVQ C   +     G  LLT  K  SAE   +T F VKD+ICERERLKETDE
Sbjct: 181 ENQMKQKSEVQGCSSSKNAETGGCCLLTGPKSNSAENGSLTPFTVKDLICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQ+LQIQAEEAQRLRRKRKADRMRLLDMQ+RQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQELQIQAEEAQRLRRKRKADRMRLLDMQKRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NINLKEQLRV+VRKELS LEM CTDMASLLRGLGI VGSG HPSSQEVQAAYKRALLKFH
Sbjct: 301 NINLKEQLRVNVRKELSKLEMNCTDMASLLRGLGIQVGSGSHPSSQEVQAAYKRALLKFH 360

Query: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKKFM 391
           PDRVSSTDIRQLVEAEEKFKLISHMKK FM
Sbjct: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKTFM 390

BLAST of MS001027 vs. ExPASy TrEMBL
Match: A0A6J1HCZ3 (uncharacterized protein LOC111462841 OS=Cucurbita moschata OX=3662 GN=LOC111462841 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.5e-167
Identity = 322/390 (82.56%), Postives = 338/390 (86.67%), Query Frame = 0

Query: 1   MAKGSHENVAFIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDD 60
           MAKGSHENVAFIDV++YRLDDVIIIDDTE V K+FQ   V  + RKSP  IYVDGCSSD+
Sbjct: 1   MAKGSHENVAFIDVENYRLDDVIIIDDTEFVTKKFQRPKVTAKSRKSPPCIYVDGCSSDE 60

Query: 61  DACSPSRTFPSSSGRHFVELDGRQSRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSS 120
           DACSPSRTFPSSS RHFVELDGRQSRK YAF MGKCR+ N GKA  RN FGLHI SHSSS
Sbjct: 61  DACSPSRTFPSSSQRHFVELDGRQSRKQYAFFMGKCRRGNCGKAPVRNHFGLHISSHSSS 120

Query: 121 SESDSSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFDV 180
           S+SDSSDCEVMEGSFE L+KQWEEASLRRKHD R AYF  DD+DKA ASH  DSDTN DV
Sbjct: 121 SDSDSSDCEVMEGSFENLRKQWEEASLRRKHDVRRAYFREDDQDKASASHRVDSDTNCDV 180

Query: 181 ENQMKQQCEVQVCPCPQTDGNDGSPLLTEIKGISAEKSPITSFDVKDIICERERLKETDE 240
           ENQMKQ+ EVQ C   +   N G  LLT  K  SAE   +T F VKD+ICERERLKETDE
Sbjct: 181 ENQMKQKSEVQACSSSKNAENGGCCLLTGPKSNSAENGSLTPFTVKDLICERERLKETDE 240

Query: 241 YRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMKRLEEVRETQKKDEE 300
           YRRAMEEEWTTRQQ+LQIQAEEAQRLRRKRKADRMRLLDMQ+RQMKRLEEVRETQKKDEE
Sbjct: 241 YRRAMEEEWTTRQQELQIQAEEAQRLRRKRKADRMRLLDMQKRQMKRLEEVRETQKKDEE 300

Query: 301 NINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQEVQAAYKRALLKFH 360
           NINLKEQLRV+VRKELS LEM CTDMASLLRGLGI VGSGLHPSSQEVQAAYKRALLKFH
Sbjct: 301 NINLKEQLRVNVRKELSKLEMNCTDMASLLRGLGIQVGSGLHPSSQEVQAAYKRALLKFH 360

Query: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKKFM 391
           PDRVSSTDIRQLVEAEEKFKLISHMKK FM
Sbjct: 361 PDRVSSTDIRQLVEAEEKFKLISHMKKTFM 390

BLAST of MS001027 vs. TAIR 10
Match: AT1G69060.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 193.7 bits (491), Expect = 2.7e-49
Identity = 106/164 (64.63%), Postives = 134/164 (81.71%), Query Frame = 0

Query: 227 DIICERERLKETDEYRRAMEEEWTTRQQQLQIQAEEAQRLRRKRKADRMRLLDMQRRQMK 286
           D++  RE LKETDEY+RA EEEW +RQ+QLQIQA+EAQ+ R++RK + MR L+M+RRQ +
Sbjct: 462 DVMLNREILKETDEYKRAQEEEWESRQRQLQIQADEAQKQRKRRKLENMRKLEMERRQKE 521

Query: 287 RLEEVRETQKKDEENINLKEQLRVDVRKELSILEMKCTDMASLLRGLGIHVGSGLHPSSQ 346
           R+EEVRETQKKDEEN+N+KE++R ++ K L +LE+K  +MA+LLRGLGI VG G+ P   
Sbjct: 522 RVEEVRETQKKDEENMNMKEKVRAEITKSLKLLELKSFNMAALLRGLGIQVGGGISPLPN 581

Query: 347 EVQAAYKRALLKFHPDRVS-STDIRQLVEAEEKFKLISHMKKKF 390
           EV AAYKRA+LKFHPDR S   DI+Q VEAEEKFKLI+ MK KF
Sbjct: 582 EVHAAYKRAVLKFHPDRASRGGDIKQQVEAEEKFKLIARMKDKF 625

HSP 2 Score: 61.6 bits (148), Expect = 1.6e-09
Identity = 58/175 (33.14%), Postives = 86/175 (49.14%), Query Frame = 0

Query: 11  FIDVDSYRLDDVIIIDDTECVNKEFQGSNVATERRKSPLYIYVDGCSSDDDACSPSRTFP 70
           FI VD    DDVII+D  E   ++  G++   +    P  I +D    DD+  +  +   
Sbjct: 40  FILVDD---DDVIILDFPESSKRKAPGTSRTRKESIMPRVISID--DDDDETENVQKAGS 99

Query: 71  SSSG--RHFVELDGRQ----SRKHYAFLMGKCRQRNYGKATARNRFGLHIGSHSSSSESD 130
           SSSG  +   E+D         K   F   KC+Q      +   RFGL   ++S  SESD
Sbjct: 100 SSSGNLQTSAEVDNDDCQFIQEKCATFRFSKCKQTFSTMPSHGIRFGLGSDTYSDFSESD 159

Query: 131 SSDCEVMEGSFEKLQKQWEEASLRRKHDARSAYFTVDDEDKAPASHGDDSDTNFD 180
            SDCE++EGS   +++QWE+A+L RK   + A      E+  P++     DTNF+
Sbjct: 160 CSDCEILEGSHRDVREQWEQAALLRK--MKKAGKAGLSEEAGPSNL--HCDTNFE 205