MS001025 (gene) Bitter gourd (TR) v1

BLAST of MS001025 vs. NCBI nr
Match: XP_022132050.1 (nucleolar protein 14 [Momordica charantia])

HSP 1 Score: 1725.3 bits (4467), Expect = 0.0e+00
Identity = 932/952 (97.90%), Postives = 938/952 (98.53%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQ-KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKG 60
           MAKLSSLSSGNNDKKNQ KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKG
Sbjct: 1   MAKLSSLSSGNNDKKNQKKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKG 60

Query: 61  EERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERK 120
           EERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERK
Sbjct: 61  EERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERK 120

Query: 121 LKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETRST--CKTFY 180
           LKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETR        Y
Sbjct: 121 LKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETRKVPFHDAQY 180

Query: 181 QQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSE 240
           QQKGGLLEG+ETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSE
Sbjct: 181 QQKGGLLEGEETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSE 240

Query: 241 ALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAMEI 300
           ALLSLTGSSNPNALKTLVQKSITNENPKKD LPAAAKTGNFNQEKPDAFDKLVKEMAMEI
Sbjct: 241 ALLSLTGSSNPNALKTLVQKSITNENPKKDNLPAAAKTGNFNQEKPDAFDKLVKEMAMEI 300

Query: 301 RARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKFIS 360
           RARPSDRTKTPEEIAQEER+RLEQLEEERQKRMLA+DGNSSDEEDEDAENASVQKRKFIS
Sbjct: 301 RARPSDRTKTPEEIAQEERDRLEQLEEERQKRMLASDGNSSDEEDEDAENASVQKRKFIS 360

Query: 361 GDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESE 420
           GDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESE
Sbjct: 361 GDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESE 420

Query: 421 EDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAYK 480
           EDDSIHGM+HSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKA+K
Sbjct: 421 EDDSIHGMKHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAFK 480

Query: 481 SAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIV 540
           +AAAKSSK TGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIV
Sbjct: 481 NAAAKSSKSTGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIV 540

Query: 541 GRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPF 600
           GRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPF
Sbjct: 541 GRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPF 600

Query: 601 YAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAIL 660
           YAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAIL
Sbjct: 601 YAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAIL 660

Query: 661 LMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSLSS 720
           LMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLV AGGRRSLSS
Sbjct: 661 LMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVVAGGRRSLSS 720

Query: 721 QNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTVIG 780
           QNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTVIG
Sbjct: 721 QNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTVIG 780

Query: 781 TLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEHYNG 840
           TLEGFVNVYGRLNSFPEIFSPIL ILHELAQQESMP VLQDKFRNVAKVIEAKTEEHYNG
Sbjct: 781 TLEGFVNVYGRLNSFPEIFSPILTILHELAQQESMPAVLQDKFRNVAKVIEAKTEEHYNG 840

Query: 841 RQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELRK 900
           RQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELRK
Sbjct: 841 RQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELRK 900

Query: 901 DNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           DNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK
Sbjct: 901 DNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 952

BLAST of MS001025 vs. NCBI nr
Match: XP_038885470.1 (nucleolar protein 14 isoform X2 [Benincasa hispida])

HSP 1 Score: 1432.9 bits (3708), Expect = 0.0e+00
Identity = 793/954 (83.12%), Postives = 852/954 (89.31%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKGE 60
           MAKLS  SS NNDKK +KKKKK SGPKAL MKV+APK NPFESIWSRRKFDVLGKKRKGE
Sbjct: 1   MAKLSIRSSSNNDKKTKKKKKKISGPKALTMKVTAPKTNPFESIWSRRKFDVLGKKRKGE 60

Query: 61  ERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERKL 120
           ERRIGLARS AIEKRKKTLLKEYEQS K+T+F+DKRIGE DEELGEFDK ILRSQRE K 
Sbjct: 61  ERRIGLARSLAIEKRKKTLLKEYEQSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 120

Query: 121 KLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP---DDDDAAAAETRSTC--KT 180
           KLNKS KYNLSDGEEDDYFG Q+LG+L A+DDFEDE+MP   DDDDAAA E +       
Sbjct: 121 KLNKSGKYNLSDGEEDDYFGIQNLGSLPANDDFEDEIMPDDDDDDDAAAGEPKKGAYRDA 180

Query: 181 FYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQ 240
            +QQKG  LEG+E KRKSKKEVMDE+IAKSKFFKAQKAKDKEENEQL+EELDKKFESLVQ
Sbjct: 181 VHQQKGPSLEGEENKRKSKKEVMDEVIAKSKFFKAQKAKDKEENEQLVEELDKKFESLVQ 240

Query: 241 SEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAM 300
           SEALLSLTGS   NALK LVQKSI+NE+ KKD L AA KT NFNQEKPDAFD+LVKEMAM
Sbjct: 241 SEALLSLTGSG--NALKALVQKSISNEHLKKDNLSAAGKTENFNQEKPDAFDRLVKEMAM 300

Query: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKF 360
           EIRARPSDRTKTPEEIAQEERERLE LEEERQKRMLA D NSSDEED+DAENASV K+K+
Sbjct: 301 EIRARPSDRTKTPEEIAQEERERLELLEEERQKRMLAPD-NSSDEEDDDAENASVGKQKY 360

Query: 361 ISGDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDE 420
           ISGDDLGDSFTLDDERSHKKGWVDEIL RKDADGTESEDDDSA+DSD+S DD D DSDDE
Sbjct: 361 ISGDDLGDSFTLDDERSHKKGWVDEILGRKDADGTESEDDDSAEDSDNSQDDADGDSDDE 420

Query: 421 SEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKA 480
           SEEDDS HG++HSLKDWEQSDDDI+DT+ ++DDE+S G   PDE+H K E+VDHEGP+KA
Sbjct: 421 SEEDDSTHGLKHSLKDWEQSDDDIIDTDSDEDDEASKGRNGPDEEHSK-EKVDHEGPKKA 480

Query: 481 YKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIIL 540
           YKS+ AKSSK  G SE+AKKLE+D +R+SK E+PYII+APES+DQF SLLA+CSNSD+IL
Sbjct: 481 YKSSIAKSSKSDGISEDAKKLERDIQRQSKPELPYIIDAPESYDQFLSLLANCSNSDVIL 540

Query: 541 IVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEI 600
           I+ RIR SNAIQL +KNLEKMQRFYGILLQYFAV ANKKPLNVELL+LLFKPLMEMSMEI
Sbjct: 541 IIARIRGSNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLFKPLMEMSMEI 600

Query: 601 PFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPA 660
           PFYAATCARMRIS THQQFCV NKNPENSSWPSSKTLILLRLWSMIFPCSDY+HVVITP 
Sbjct: 601 PFYAATCARMRISRTHQQFCVQNKNPENSSWPSSKTLILLRLWSMIFPCSDYNHVVITPT 660

Query: 661 ILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSL 720
           ILLMCEYLMRCPIVTGRD+A G+FLCSLLLYVARQS KFCPEAINFLRTLL AA  R S 
Sbjct: 661 ILLMCEYLMRCPIVTGRDLATGAFLCSLLLYVARQSSKFCPEAINFLRTLLAAAVSRSS- 720

Query: 721 SSQNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTV 780
           SSQNPQICHLVDLQALG LL IQNP NEITPLNFFFIMDLT+DSS FSSDNFRAGLL TV
Sbjct: 721 SSQNPQICHLVDLQALGQLLRIQNPTNEITPLNFFFIMDLTDDSSVFSSDNFRAGLLSTV 780

Query: 781 IGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEHY 840
             TL GFVN+YG+L +FPEIF PI  ILHELAQQE+MPDVLQDKFR VAK IEAKTEEHY
Sbjct: 781 TETLYGFVNIYGQLKTFPEIFLPISTILHELAQQENMPDVLQDKFRKVAKAIEAKTEEHY 840

Query: 841 NGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAAREL 900
            GRQPLRMRKQKAVPIKLLNPKFEENFV+GRDYDPDRER+ERRK+ KL+KRE KGA REL
Sbjct: 841 TGRQPLRMRKQKAVPIKLLNPKFEENFVRGRDYDPDRERAERRKMQKLLKRETKGAVREL 900

Query: 901 RKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           RKDNHFL EVKARDKALQEEERAEKY +AR FL+ QEHAFKSGQL G GRKRRK
Sbjct: 901 RKDNHFLSEVKARDKALQEEERAEKYKKARTFLEAQEHAFKSGQL-GNGRKRRK 948

BLAST of MS001025 vs. NCBI nr
Match: XP_038885469.1 (nucleolar protein 14 isoform X1 [Benincasa hispida])

HSP 1 Score: 1428.3 bits (3696), Expect = 0.0e+00
Identity = 793/955 (83.04%), Postives = 852/955 (89.21%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKGE 60
           MAKLS  SS NNDKK +KKKKK SGPKAL MKV+APK NPFESIWSRRKFDVLGKKRKGE
Sbjct: 1   MAKLSIRSSSNNDKKTKKKKKKISGPKALTMKVTAPKTNPFESIWSRRKFDVLGKKRKGE 60

Query: 61  ERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERKL 120
           ERRIGLARS AIEKRKKTLLKEYEQS K+T+F+DKRIGE DEELGEFDK ILRSQRE K 
Sbjct: 61  ERRIGLARSLAIEKRKKTLLKEYEQSRKSTEFSDKRIGEWDEELGEFDKAILRSQRELKR 120

Query: 121 KLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP---DDDDAAAAETRSTC--KT 180
           KLNKS KYNLSDGEEDDYFG Q+LG+L A+DDFEDE+MP   DDDDAAA E +       
Sbjct: 121 KLNKSGKYNLSDGEEDDYFGIQNLGSLPANDDFEDEIMPDDDDDDDAAAGEPKKGAYRDA 180

Query: 181 FYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQ 240
            +QQKG  LEG+E KRKSKKEVMDE+IAKSKFFKAQKAKDKEENEQL+EELDKKFESLVQ
Sbjct: 181 VHQQKGPSLEGEENKRKSKKEVMDEVIAKSKFFKAQKAKDKEENEQLVEELDKKFESLVQ 240

Query: 241 SEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAM 300
           SEALLSLTGS   NALK LVQKSI+NE+ KKD L AA KT NFNQEKPDAFD+LVKEMAM
Sbjct: 241 SEALLSLTGSG--NALKALVQKSISNEHLKKDNLSAAGKTENFNQEKPDAFDRLVKEMAM 300

Query: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKF 360
           EIRARPSDRTKTPEEIAQEERERLE LEEERQKRMLA D NSSDEED+DAENASV K+K+
Sbjct: 301 EIRARPSDRTKTPEEIAQEERERLELLEEERQKRMLAPD-NSSDEEDDDAENASVGKQKY 360

Query: 361 ISGDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDE 420
           ISGDDLGDSFTLDDERSHKKGWVDEIL RKDADGTESEDDDSA+DSD+S DD D DSDDE
Sbjct: 361 ISGDDLGDSFTLDDERSHKKGWVDEILGRKDADGTESEDDDSAEDSDNSQDDADGDSDDE 420

Query: 421 SEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKA 480
           SEEDDS HG++HSLKDWEQSDDDI+DT+ ++DDE+S G   PDE+H K E+VDHEGP+KA
Sbjct: 421 SEEDDSTHGLKHSLKDWEQSDDDIIDTDSDEDDEASKGRNGPDEEHSK-EKVDHEGPKKA 480

Query: 481 YKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIIL 540
           YKS+ AKSSK  G SE+AKKLE+D +R+SK E+PYII+APES+DQF SLLA+CSNSD+IL
Sbjct: 481 YKSSIAKSSKSDGISEDAKKLERDIQRQSKPELPYIIDAPESYDQFLSLLANCSNSDVIL 540

Query: 541 IVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEI 600
           I+ RIR SNAIQL +KNLEKMQRFYGILLQYFAV ANKKPLNVELL+LLFKPLMEMSMEI
Sbjct: 541 IIARIRGSNAIQLTDKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLFKPLMEMSMEI 600

Query: 601 PFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPA 660
           PFYAATCARMRIS THQQFCV NKNPENSSWPSSKTLILLRLWSMIFPCSDY+HVVITP 
Sbjct: 601 PFYAATCARMRISRTHQQFCVQNKNPENSSWPSSKTLILLRLWSMIFPCSDYNHVVITPT 660

Query: 661 ILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSL 720
           ILLMCEYLMRCPIVTGRD+A G+FLCSLLLYVARQS KFCPEAINFLRTLL AA  R S 
Sbjct: 661 ILLMCEYLMRCPIVTGRDLATGAFLCSLLLYVARQSSKFCPEAINFLRTLLAAAVSRSS- 720

Query: 721 SSQNP-QICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLT 780
           SSQNP QICHLVDLQALG LL IQNP NEITPLNFFFIMDLT+DSS FSSDNFRAGLL T
Sbjct: 721 SSQNPQQICHLVDLQALGQLLRIQNPTNEITPLNFFFIMDLTDDSSVFSSDNFRAGLLST 780

Query: 781 VIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEH 840
           V  TL GFVN+YG+L +FPEIF PI  ILHELAQQE+MPDVLQDKFR VAK IEAKTEEH
Sbjct: 781 VTETLYGFVNIYGQLKTFPEIFLPISTILHELAQQENMPDVLQDKFRKVAKAIEAKTEEH 840

Query: 841 YNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARE 900
           Y GRQPLRMRKQKAVPIKLLNPKFEENFV+GRDYDPDRER+ERRK+ KL+KRE KGA RE
Sbjct: 841 YTGRQPLRMRKQKAVPIKLLNPKFEENFVRGRDYDPDRERAERRKMQKLLKRETKGAVRE 900

Query: 901 LRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           LRKDNHFL EVKARDKALQEEERAEKY +AR FL+ QEHAFKSGQL G GRKRRK
Sbjct: 901 LRKDNHFLSEVKARDKALQEEERAEKYKKARTFLEAQEHAFKSGQL-GNGRKRRK 949

BLAST of MS001025 vs. NCBI nr
Match: XP_022951529.1 (nucleolar protein 14 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1427.5 bits (3694), Expect = 0.0e+00
Identity = 797/955 (83.46%), Postives = 858/955 (89.84%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQ--KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRK 60
           MAKLS+LSS NNDKK++  KKKKKS+GPKAL+MKVSAPK NPFESIWSRRKFDVLGKKRK
Sbjct: 1   MAKLSNLSSSNNDKKDKKSKKKKKSAGPKALSMKVSAPKANPFESIWSRRKFDVLGKKRK 60

Query: 61  GEERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRER 120
           GEERRIGLARS AIEKRKKTLL+EYEQSGK+T+F+DKRIGEQDEELGEFDK ILRSQRER
Sbjct: 61  GEERRIGLARSIAIEKRKKTLLREYEQSGKSTEFSDKRIGEQDEELGEFDKAILRSQRER 120

Query: 121 KLKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP-DDDDAAAAETRSTC--KT 180
           KLKLNKSSK+NLSDGE+DDYFGS SLGAL A+DDFEDEV+P DDDDA AAET+       
Sbjct: 121 KLKLNKSSKFNLSDGEDDDYFGSNSLGALPANDDFEDEVIPDDDDDAEAAETKKGAYHGA 180

Query: 181 FYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQ 240
            Y QKGGLLEG+E KRKSKKEVMDEIIAKSKFFKAQKA+DKEENEQLIE+LDKKFESLVQ
Sbjct: 181 PYPQKGGLLEGEENKRKSKKEVMDEIIAKSKFFKAQKARDKEENEQLIEDLDKKFESLVQ 240

Query: 241 SEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAM 300
           SEALLSLT S N NALK LVQKSI NE+ KKD L A  KT NFNQEKPDAFDKLVKEMAM
Sbjct: 241 SEALLSLTSSGNANALKALVQKSIPNEHLKKDNLLATGKTENFNQEKPDAFDKLVKEMAM 300

Query: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKF 360
           EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLA D NSSD+ED DAENASVQK+KF
Sbjct: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLAPD-NSSDDEDGDAENASVQKQKF 360

Query: 361 ISGDDLGDSFTL-DDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDD 420
           ISGDDLGDSFTL DDER+HKKGWVDEI ERKDADGTESEDDDSA+DSDSS DD   DSDD
Sbjct: 361 ISGDDLGDSFTLDDDERNHKKGWVDEIFERKDADGTESEDDDSAEDSDSS-DDVGGDSDD 420

Query: 421 ESEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQK 480
           ESEEDD+  G +HSLKDWEQSDDDILD+N E+DDE+S  +KE DE+HPK          K
Sbjct: 421 ESEEDDNTRGRKHSLKDWEQSDDDILDSNSEEDDEASKEDKELDENHPK----------K 480

Query: 481 AYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDII 540
           A K A  KSSK  G SE+AKKLEK+TKR++K E+PYIIEAPESFDQF SLLADCS+SD+I
Sbjct: 481 ANKGAIVKSSKSEGSSEDAKKLEKNTKRENKPELPYIIEAPESFDQFLSLLADCSDSDVI 540

Query: 541 LIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSME 600
           LI+ RIRASNAIQL EKNLEKMQRFYGILLQYFAV ANKKPLNVELL+LL K LMEMS +
Sbjct: 541 LIIDRIRASNAIQLTEKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLLKSLMEMSRQ 600

Query: 601 IPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660
           IPFYAATCAR RISHTHQQFCV NKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP
Sbjct: 601 IPFYAATCARTRISHTHQQFCVDNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660

Query: 661 AILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRS 720
           AILLMCEYLMRCPIVTGRDIA+GSFLCSLLLYVA+QSLKFCPEAINFL+TLL+AA GRRS
Sbjct: 661 AILLMCEYLMRCPIVTGRDIAIGSFLCSLLLYVAKQSLKFCPEAINFLQTLLIAAAGRRS 720

Query: 721 LSSQNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLT 780
           L SQNPQIC+LVDL ALG LL IQNP NEITPL+FFF+M+LTE SS FSSD++RAG+LLT
Sbjct: 721 LPSQNPQICNLVDLHALGQLLRIQNPTNEITPLDFFFMMNLTEHSSIFSSDSYRAGVLLT 780

Query: 781 VIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEH 840
           VI TL+GFVNVYG+L SFPEIF P   ILHELA QE+MPDVL+DKFR VA+ IEAKTEEH
Sbjct: 781 VIETLDGFVNVYGQLKSFPEIFMPFSTILHELALQENMPDVLRDKFRKVAEAIEAKTEEH 840

Query: 841 YNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARE 900
           Y GRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRER E+RKL KL+KREAKGAARE
Sbjct: 841 YMGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERVEKRKLQKLLKREAKGAARE 900

Query: 901 LRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           LRKDNHFLF+VKARDKALQEEERAE+Y +A AFLQ+QEHAFKSGQL GKGRKRRK
Sbjct: 901 LRKDNHFLFDVKARDKALQEEERAERYRKASAFLQQQEHAFKSGQL-GKGRKRRK 942

BLAST of MS001025 vs. NCBI nr
Match: XP_023537551.1 (nucleolar protein 14 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1427.5 bits (3694), Expect = 0.0e+00
Identity = 796/955 (83.35%), Postives = 860/955 (90.05%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQ--KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRK 60
           MAKLS+LSS NNDKK++  KKKKKS+GPKAL+MKVSAPK NPFESIWSRRKFDVLGKKRK
Sbjct: 1   MAKLSNLSSSNNDKKDKKSKKKKKSAGPKALSMKVSAPKANPFESIWSRRKFDVLGKKRK 60

Query: 61  GEERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRER 120
           GEERRIGLARS AIEKRKKTLL+EYEQSGK+T+F+DKRIGEQDEELGEFDK ILRSQRER
Sbjct: 61  GEERRIGLARSLAIEKRKKTLLREYEQSGKSTEFSDKRIGEQDEELGEFDKAILRSQRER 120

Query: 121 KLKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP-DDDDAAAAETRSTC--KT 180
           KLKLNKSSK+NLSDGE+DDYFGS SLGAL A+DDFEDEV+P DDDDA AAET+       
Sbjct: 121 KLKLNKSSKFNLSDGEDDDYFGSNSLGALPANDDFEDEVIPDDDDDAEAAETKKGAYHGA 180

Query: 181 FYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQ 240
            YQQK GLLEG+E KRKSKKEVMDEIIAKSKFFKAQKA+DKEENEQLIE+LDKKFESLVQ
Sbjct: 181 PYQQKSGLLEGEENKRKSKKEVMDEIIAKSKFFKAQKARDKEENEQLIEDLDKKFESLVQ 240

Query: 241 SEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAM 300
           SEALLSLT S N NALK LVQKSI NE+ KKD L +A KT NFNQEKPDAFDKLVKEMAM
Sbjct: 241 SEALLSLTSSGNANALKALVQKSIPNEHLKKDNLLSAGKTENFNQEKPDAFDKLVKEMAM 300

Query: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKF 360
           EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLA D NSSD+ED DAENASVQKRKF
Sbjct: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLAPD-NSSDDEDGDAENASVQKRKF 360

Query: 361 ISGDDLGDSFTL-DDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDD 420
           ISGDDLGDSFTL DDER+HKKGWVDEI ERKDADGTESEDDDSA+D+DSS DD   DSDD
Sbjct: 361 ISGDDLGDSFTLDDDERNHKKGWVDEIFERKDADGTESEDDDSAEDTDSS-DDVGGDSDD 420

Query: 421 ESEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQK 480
           ESEEDD+  G +HSLKDWEQSDDDILD+N E+DDE+S  +KE DE+HPK          K
Sbjct: 421 ESEEDDNTRGRKHSLKDWEQSDDDILDSNSEEDDEASKEDKELDENHPK----------K 480

Query: 481 AYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDII 540
           A K A  KSSK  G SE+AKKLEK+TKR++K E+PYIIEAPESFDQF SLLADCS+SD++
Sbjct: 481 ANKGAIIKSSKSEGSSEDAKKLEKNTKRENKPELPYIIEAPESFDQFLSLLADCSDSDVL 540

Query: 541 LIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSME 600
           LI+ RIRASNAIQL EKNLEKMQRFYGILLQYFAV ANKKPLNVELL+LL KPLMEMS +
Sbjct: 541 LIIDRIRASNAIQLTEKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLLKPLMEMSRQ 600

Query: 601 IPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660
           IPFYAATCAR RISHTHQQFCV NKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP
Sbjct: 601 IPFYAATCARTRISHTHQQFCVDNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660

Query: 661 AILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRS 720
           AILLMCEYLMRCPIVTGRDIA+GSFLCSLLLYVA+QSLKFCPEAINFL+TLL+AA GRRS
Sbjct: 661 AILLMCEYLMRCPIVTGRDIAIGSFLCSLLLYVAKQSLKFCPEAINFLQTLLIAAAGRRS 720

Query: 721 LSSQNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLT 780
           L SQNPQIC+LVDLQALG LL IQNP NEITPL+FFF+M+LTE SS FSSD++RAG+LLT
Sbjct: 721 LPSQNPQICNLVDLQALGQLLRIQNPTNEITPLDFFFMMNLTEHSSIFSSDSYRAGVLLT 780

Query: 781 VIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEH 840
           VI TL+GFVNVYG+L SFPEIF P   ILHELA QE+MPDVL+DKF  VA+ IEAKTEE+
Sbjct: 781 VIETLDGFVNVYGQLKSFPEIFMPFSTILHELALQENMPDVLRDKFSKVAEAIEAKTEEY 840

Query: 841 YNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARE 900
           Y GRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRER E+RKL KL+KREAKGAARE
Sbjct: 841 YMGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERVEKRKLQKLLKREAKGAARE 900

Query: 901 LRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           LRKDNHFLF+VKARDKALQEEERAE+Y +A AFLQ+QEHAFKSGQL GKGRKRRK
Sbjct: 901 LRKDNHFLFDVKARDKALQEEERAERYKKASAFLQQQEHAFKSGQL-GKGRKRRK 942

BLAST of MS001025 vs. ExPASy Swiss-Prot
Match: Q8R3N1 (Nucleolar protein 14 OS=Mus musculus OX=10090 GN=Nop14 PE=1 SV=2)

HSP 1 Score: 273.1 bits (697), Expect = 1.2e-71
Identity = 279/952 (29.31%), Postives = 463/952 (48.63%), Query Frame = 0

Query: 14  KKNQKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKGEERRIGLARSHAIE 73
           K+   +++    P   +   +   PNPFE   +R+KF +LG+K + +    G++R+ AI 
Sbjct: 5   KRTGARRQVHKAPAGASGGPAKTNPNPFEVKVNRQKFQILGRKTRHDVGLPGVSRARAIR 64

Query: 74  KRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERKLKLNKSSKYNLSDG 133
           KR +TLLKEY++  K+  F DKR GE +  +   +K + R   E++    K + YNL++ 
Sbjct: 65  KRTQTLLKEYKERNKSNVFADKRFGEYNSNISPEEKMMKRFALEQQRYHEKKNIYNLNED 124

Query: 134 EEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETRSTCKTF---YQQKGGLLEG-DET 193
           EE  ++G QSL  +   +D  D    D +D  A     T   F     +     EG D  
Sbjct: 125 EELTHYG-QSLADIEKHNDIVDS-DSDTEDRGALSAELTASHFGGGVHKNSSQKEGEDGD 184

Query: 194 KRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSEALLSLTGSSNPN 253
           K K++KE+++E+IAKSK  K ++   +E+  +L E+LD+ ++ +   + L+S        
Sbjct: 185 KPKTRKELIEELIAKSKQEKRERQAQREDALELTEKLDQDWKEI---QILMS-------- 244

Query: 254 ALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAMEIRARPSDRTKTPE 313
                 +K   +E+ +K   P           +PD +D +V+E+  E++A+PS+R KT E
Sbjct: 245 ------RKPKKSEDKEKKEKP-----------QPDEYDMMVRELGFEMKAQPSNRMKTEE 304

Query: 314 EIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKFISGDDLGDSFTLD- 373
           E+A+EE+ERL++LE ER +RML  D           E+ + +K K  S DDL D F LD 
Sbjct: 305 ELAKEEQERLKKLEAERLRRMLGKD-----------EHENKKKPKHTSADDLNDGFILDK 364

Query: 374 DER---SHKKGW--VDEILER--KDADGTESEDDDSADDS---DSSHDDRDEDSDDESEE 433
           D+R   S+K G   ++++ E   K+ADG E++  +  DDS   D SH+D +E  D +S  
Sbjct: 365 DDRRLLSYKDGKMNIEDVQEEQSKEADGQENDQKEGEDDSEEEDESHEDSEESEDPDSHS 424

Query: 434 DDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKE----PDEDHPKEEEVDHEGPQK 493
           D                    L++N+E ++E+   +KE    P    PK++       QK
Sbjct: 425 D--------------------LESNIESEEENETPKKEQRQTPGGKLPKDD-------QK 484

Query: 494 AYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDII 553
           A K+ AA                         E+PY+  APESF++   LL+  S  + +
Sbjct: 485 AQKAVAA-------------------------ELPYVFAAPESFEELKFLLSGRSMEEQL 544

Query: 554 LIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSME 613
           L+V RI+  N   L   N  K+++ +G LLQY   LA     +++ +  L   L  +   
Sbjct: 545 LVVERIQKCNHPSLAVGNKAKLEKLFGFLLQYIGDLATDSTPDLKTIDKLVVHLYSLCQM 604

Query: 614 IPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 673
            P  A+   R  +     +     +    +++P    LI L++  ++FP SD+ H V+TP
Sbjct: 605 FPESASDSIRFVLRDAMHEMEEMIETKGRAAFPGLDVLIYLKITGLLFPTSDFWHPVVTP 664

Query: 674 AILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRS 733
           A+L M + L +CP+++ +D+  G F+C L L     S +F PE  NFL  +L  A    +
Sbjct: 665 ALLCMSQMLTKCPVMSLQDVIKGLFVCCLFLEYVSLSRRFIPELFNFLLGILYIA--TPN 724

Query: 734 LSSQNPQICHLVDLQALG---PLLHIQNPINEIT------PLNFFFIMDLTEDSSFFSSD 793
             SQ   + H    +ALG    LL + +  +  T      PL++    +     +   ++
Sbjct: 725 TKSQGSTLVH--PFRALGKNSELLVVSDKADVTTWQRGSLPLHW---ANRLSTLTATEAN 784

Query: 794 NFRAGLLLTVIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAK 853
           + R   + + +  ++  V +Y  L SF  IF P  A+L +     S+P  LQ+  +++  
Sbjct: 785 HTRLSCVASCLSLMKHCVLMYQALPSFHAIFRPHQALLSKHLADCSLPQELQELAQSILS 844

Query: 854 VIEAKTEEHYNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIK 913
            +E + +      +PL   K K VP+K   P+  +    GR     +E  ER++L    K
Sbjct: 845 AMEGQKQH----CRPLVCEKSKPVPLKQFTPRLVKVLEFGRKQGSSKEEQERKRLIHKHK 852

Query: 914 REAKGAARELRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKS 938
           RE KGA RE+RKDN FL  ++  +   ++ ER  K  +    L  QE  +K+
Sbjct: 905 REFKGAVREIRKDNQFLARMQLSEIMERDAERKRKVKQLFNSLATQEGEWKA 852

BLAST of MS001025 vs. ExPASy Swiss-Prot
Match: P78316 (Nucleolar protein 14 OS=Homo sapiens OX=9606 GN=NOP14 PE=1 SV=3)

HSP 1 Score: 270.4 bits (690), Expect = 7.7e-71
Identity = 286/941 (30.39%), Postives = 454/941 (48.25%), Query Frame = 0

Query: 14  KKNQKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKGEERRIGLARSHAIE 73
           KK   ++K S  P       +    NPFE   +R+KF +LG+K + +    G++R+ A+ 
Sbjct: 5   KKVGARRKASGAPAGARGGPAKANSNPFEVKVNRQKFQILGRKTRHDVGLPGVSRARALR 64

Query: 74  KRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERKLKLNKSSKYNLSDG 133
           KR +TLLKEY++  K+  F DKR GE +  +   +K + R   E++    K S YNL++ 
Sbjct: 65  KRTQTLLKEYKERDKSNVFRDKRFGEYNSNMSPEEKMMKRFALEQQRHHEKKSIYNLNED 124

Query: 134 EEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETRSTCKT--FYQQKGGLL------E 193
           EE  ++G QSL  +   +D  D     D DA    T S   T   +   GGLL      E
Sbjct: 125 EELTHYG-QSLADIEKHNDIVD----SDSDAEDRGTLSAELTAAHFGGGGGLLHKKTQQE 184

Query: 194 GDE-TKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSEALLSLTG 253
           G+E  K KS+KE+++E+IAKSK  K ++   +E+  +L E+LD+ ++ +   + LLS   
Sbjct: 185 GEEREKPKSRKELIEELIAKSKQEKRERQAQREDALELTEKLDQDWKEI---QTLLS--- 244

Query: 254 SSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAMEIRARPSDR 313
                       K+  +EN  K   P           KPDA+D +V+E+  E++A+PS+R
Sbjct: 245 -----------HKTPKSENRDKKEKP-----------KPDAYDMMVRELGFEMKAQPSNR 304

Query: 314 TKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKFISGDDLGDS 373
            KT  E+A+EE+E L +LE ER +RML  D           E+ +V+K K +S DDL D 
Sbjct: 305 MKTEAELAKEEQEHLRKLEAERLRRMLGKD-----------EDENVKKPKHMSADDLNDG 364

Query: 374 FTLD-DERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESEEDDSIH 433
           F LD D+R         +L  KD      ED       ++S      D +   EE DS  
Sbjct: 365 FVLDKDDR--------RLLSYKDGKMNVEEDVQEEQSKEAS------DPESNEEEGDSSG 424

Query: 434 GMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAYKSAAAKS 493
           G     +D E+SD      +LE + ES       +E++ K  +   + P K   S   ++
Sbjct: 425 G-----EDTEESDSPDSHLDLESNVES-------EEENEKPAKEQRQTPGKGLISGKERA 484

Query: 494 SKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIVGRIRAS 553
            K T                 ++E+PY   APES+++  SLL   S  + +L+V RI+  
Sbjct: 485 GKAT-----------------RDELPYTFAAPESYEELRSLLLGRSMEEQLLVVERIQKC 544

Query: 554 NAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPFYAATCA 613
           N   L E N  K+++ +G LL+Y   LA   P ++ ++  L   L  +    P  A+   
Sbjct: 545 NHPSLAEGNKAKLEKLFGFLLEYVGDLATDDPPDLTVIDKLVVHLYHLCQMFPESASDAI 604

Query: 614 RMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAILLMCEYL 673
           +  +     +     +    ++ P    LI L++  ++FP SD+ H V+TPA++ + + L
Sbjct: 605 KFVLRDAMHEMEEMIETKGRAALPGLDVLIYLKITGLLFPTSDFWHPVVTPALVCLSQLL 664

Query: 674 MRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSLSSQNPQIC 733
            +CPI++ +D+  G F+C L L     S +F PE INFL  +L  A   +  +SQ   + 
Sbjct: 665 TKCPILSLQDVVKGLFVCCLFLEYVALSQRFIPELINFLLGILYIATPNK--ASQGSTLV 724

Query: 734 HLVDLQALG---PLLHIQNPINEIT----PLNFFFIMDLTEDSSFFSSDNFRAGLLLTVI 793
           H    +ALG    LL +    +  T     L+  +   L   +S   +++ R   L   +
Sbjct: 725 H--PFRALGKNSELLVVSAREDVATWQQSSLSLRWASRLRAPTS-TEANHIRLSCLAVGL 784

Query: 794 GTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEHYN 853
             L+  V +YG L SF  I  P+ A+L +     S P  LQ+  ++    +E++ +    
Sbjct: 785 ALLKRCVLMYGSLPSFHAIMGPLQALLTDHLADCSHPQELQELCQSTLTEMESQKQL--- 844

Query: 854 GRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELR 913
             +PL   K K VP+KL  P+  +    GR     +E  ER++L    KRE KGA RE+R
Sbjct: 845 -CRPLTCEKSKPVPLKLFTPRLVKVLEFGRKQGSSKEEQERKRLIHKHKREFKGAVREIR 849

Query: 914 KDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKS 938
           KDN FL  ++  +   ++ ER  K  +    L  QE  +K+
Sbjct: 905 KDNQFLARMQLSEIMERDAERKRKVKQLFNSLATQEGEWKA 849

BLAST of MS001025 vs. ExPASy Swiss-Prot
Match: Q9VEJ2 (Nucleolar protein 14 homolog OS=Drosophila melanogaster OX=7227 GN=l(3)07882 PE=2 SV=2)

HSP 1 Score: 162.9 bits (411), Expect = 1.7e-38
Identity = 245/956 (25.63%), Postives = 423/956 (44.25%), Query Frame = 0

Query: 18  KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKGEERRIGLARSHAIEKRKK 77
           KK  +S+ P   +   S+ + NPF+   ++ KF +LG+  K +    G++R+ A++KR +
Sbjct: 16  KKTTRSANPFDNSTAQSSKRGNPFDVHVNKEKFKILGRICKHDRGMPGVSRAKALQKRAQ 75

Query: 78  TLLKEYEQSGKATKFTDKRIGE--QDEELGE--FDKGILRSQRERKLKLNKSSKYNLSDG 137
           TL +++    K  +F D RIG+    ++L E   +   L  +  +     K+ K+NL+D 
Sbjct: 76  TLGQQFAVKHKTNRFKDNRIGKHLSGDQLTESVMNARYLAEKMSQVRSNQKAEKFNLNDD 135

Query: 138 EEDDYFGSQSLGALTASDDFEDEVMPDDD-DAAAAETRSTCKTFYQQKGGLLEGDETKRK 197
           E   + G      L   + + DE   D++ D  A +   T    +   GG  EGD    +
Sbjct: 136 ELLTHRGQ----TLEEIEQYRDERSDDEELDDEALDADFTAAAHF---GG--EGDSA--Q 195

Query: 198 SKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSEALLSLTGSSNPNALK 257
            ++  +DE+I + K  K + AK+K+E   L E+LD  ++ L+                  
Sbjct: 196 DRQAAIDEMIVEQKRRKNEIAKEKDEVYDLTEKLDANYKLLL------------------ 255

Query: 258 TLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAMEIRARPSDRTKTPEEIA 317
            LV K   +E   K                PDA+DKL+KEM  E R   +D+   P+E+A
Sbjct: 256 PLVAKVTKDEQDAK--------------PPPDAYDKLLKEMIFEPRGSVADKLINPDELA 315

Query: 318 QEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKFISGDDLGDSFTLDDERS 377
           ++E  RLE+LE ER +RM A DG    EEDE+   ASV K K  S DDL D + L  E  
Sbjct: 316 KQEAARLEKLENERLRRMRA-DG----EEDEE---ASVAKPKHRSADDLDDGYFLAGE-- 375

Query: 378 HKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESEEDDSIHGMEHSLKDW 437
                                 DD  DD+ +   D +  +    +++  + G        
Sbjct: 376 ----------------------DDEGDDTLAYDLDGNLGTHLNGKKEAVLKG-------- 435

Query: 438 EQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAYKSAAAKSSKITGHSEE 497
                   D N +DDD+    E+E D D   + EVD+    K  +S +          ++
Sbjct: 436 --------DENEDDDDKEGEEEEEEDSDEESDSEVDNLSDLKESESESEPEEAPKASKKK 495

Query: 498 AKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIVGRIRASNAIQLKEKN 557
           AKK            IP+ I+ P++++ F+ LL+  + +   +I+ RI   N  +L+  N
Sbjct: 496 AKKSADTIPASLDTSIPFTIKMPKTYEDFTELLSKHATAQKAIIIERIIKCNHPKLEGVN 555

Query: 558 LEKMQRFYGILLQYFAVL---ANKKPL--NVELLSLLFKPLMEMSMEIPFYAATCARMRI 617
            E + + Y  LLQY   L   A+++ +  + +LLS L   L E++   P   +      I
Sbjct: 556 RENVVKLYSFLLQYLKDLFEDASEQDIREHFQLLSKLMPYLYELTQLNPERMSNTLLEVI 615

Query: 618 SHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAILLMCEYLMRCP 677
              +++F  ++K      +PS  TL+  +L + ++  SD+ H V+TP  + +   L R  
Sbjct: 616 KEKYEEFRKNHK-----MYPSLDTLVYFKLVANLYSTSDFRHPVVTPCFIFIQHVLSRSR 675

Query: 678 IVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSLSSQNPQICHLVD 737
           + T ++I++G FL +++L    QS +  P   NFL+ ++  +  +R +            
Sbjct: 676 VRTRQEISMGLFLVTVVLEFVSQSKRLVPAVFNFLQGIVHMSIPKRDVEQ---------- 735

Query: 738 LQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFS--------------SDNFRAGLLL 797
                  L I  P     PL+    +    +S+                 + +F+   L 
Sbjct: 736 -------LEITPPFERDGPLSKLLALPANTESTKLEPQQLQPTDLVTQTITPDFKVRALD 795

Query: 798 TVIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEE 857
           T +  ++  + +         +  P LA+L  L   ES P+ +    ++  ++ E    +
Sbjct: 796 TSLLLIKEALQLVEEHVGACYLAQPFLALLSRL-PLESYPEHVHQHHKDATELAEKLAAQ 849

Query: 858 HYNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAAR 917
                +PL   ++K   ++LL P+FE  +   R     + + ER KL   IKRE KGA R
Sbjct: 856 K---MKPLAPAEKKPKALRLLEPRFEAVYDDKRRPKMSKAKEERAKLLHKIKREKKGAIR 849

Query: 918 ELRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           E+R+D  F+  ++ +     ++ER EK  R       QE + + G+L    R ++K
Sbjct: 916 EIRRDTSFVQMLRLKQTLQSDKERHEKVKRI-----YQEASVQQGELNELSRAKKK 849

BLAST of MS001025 vs. ExPASy Swiss-Prot
Match: Q6C2F4 (Probable nucleolar complex protein 14 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=NOP14 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 9.6e-37
Identity = 243/973 (24.97%), Postives = 411/973 (42.24%), Query Frame = 0

Query: 3   KLSSLSSGNNDKKNQKKKK--KSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKGE 62
           K + L+   N K N+KK    K+   +   +     + NPF+   +R+K D+ G+  +G 
Sbjct: 15  KKNDLTGQTNVKGNKKKMNSGKNRQDRDKVLSDIRQQFNPFDVKVARKKHDIGGRTVRGS 74

Query: 63  ERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERKL 122
             R GL++    E R +    E +  G+     D+R GE D  +   +K + R  RER+L
Sbjct: 75  VGRPGLSKLSGEEARMEARKLELQNKGRVGGVFDRRFGEGDSNMNPEEKMLERFTRERQL 134

Query: 123 KL----NKSSKYNLSDGEED---DYFGSQSLGALTASDDFED---EVMPDDDDAAAAETR 182
           +        S + L D ++D   D   + S  AL   DDF+     +  ++D+  AA   
Sbjct: 135 RSMGGGRNKSIFALDDDDDDADADMMLTHSGQALDFKDDFDQGDLGIEAEEDEEMAAILA 194

Query: 183 STCKTFYQQKGGLL-------------EGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKE 242
           +  K   ++  G +             E    ++KSK+EVM EIIAKSKF KA++   ++
Sbjct: 195 NRRKLAEERGMGAMGVNLEEMDGVDMEEAGTGRKKSKEEVMKEIIAKSKFHKAERQAARD 254

Query: 243 ENEQLIEELDKKFESLVQSEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGN 302
           +++ +IEE++ +        AL+   GS     + T +                      
Sbjct: 255 KDQAIIEEMNDE----DTMNALIRELGSIKAKKVVTAL---------------------- 314

Query: 303 FNQEKPDAFDKLVKEMAMEIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNS 362
              +K   +D+  + M ++ RA+P DRTKT EE+A+EE E+L++LE+ERQ RM    G  
Sbjct: 315 --DQKEKEYDQNFRNMILDRRAKPQDRTKTDEELAKEEAEKLKKLEDERQARM---RGEV 374

Query: 363 SDEEDEDAENASVQKRKFISGDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDS 422
           + ++ E              GDDL  +   D E S ++G                     
Sbjct: 375 AIDQGE--------------GDDLDGNVAFDFENSEEEG--------------------- 434

Query: 423 ADDSDSSHDDRDEDSDDESEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEP 482
                       ED +DE+E+D                DD+ ++ +  D+DE   G+K  
Sbjct: 435 ------------EDDEDEAEDD----------------DDEDVEIHEVDEDEDESGDKSG 494

Query: 483 DEDHPKEEEVDHEGPQKAYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPES 542
           DE    EEE     P       A+KSS  T                    + Y    P+S
Sbjct: 495 DES--GEEETTKSEP-------ASKSSSST--------------------LAYTFPIPKS 554

Query: 543 FDQFSSLLADCSNSDIILIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLN 602
              F    +      +  I+ RI   +   LKE N E++ +F  +L+ +   LA+++  +
Sbjct: 555 HKMFLETTSAYPLDQLPTIIDRINVLHHASLKEGNKERLAKFACVLIDHLMYLADEEEED 614

Query: 603 VELLSLLFKPLMEMSMEIPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRL 662
                  F+ L E+  ++   A T +    +H  ++   H    +  +  ++  L+L  L
Sbjct: 615 D------FECLSEVVAKVHTLAETHSPTMAAHIRKKLEAH----QADTTVTAGHLMLWTL 674

Query: 663 WSMIFPCSDYHHVVITPAILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPE 722
             MIF  SD+ H+V+TPA+L+M  +L      +      G +   LL+   R + +F PE
Sbjct: 675 IGMIFSTSDHFHLVVTPAVLVMTRFLSLSTFDSIPKCIAGLYTAQLLIQYQRIAKRFIPE 734

Query: 723 AINFLRTLLVAAGGRRSLSSQNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTE 782
              FL  L+ A  G  + S     +  +  ++   P        ++   +       L++
Sbjct: 735 IAVFLGRLIAALEGTET-SLPMASLFKIAPIKGFTPGKASHKSTDKTISMRSANRSILSK 794

Query: 783 DSSFFSSDNFRAGLLLTVIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQ 842
                 S       + +V       ++ Y  +++FPE F             E++P+ L 
Sbjct: 795 KEVATLSQELHNQAVFSV----SKLMDTYKDISAFPETFEFC----------ENIPE-LA 831

Query: 843 DKFRNVAKVIEAKTEEHYNGRQPLRMRKQKAVPIKLLNPKFEENF-VKGRDYDPDRERSE 902
           DK+  + K   A        R+PL + K + + IK + PKFEENF V  + Y+PD    E
Sbjct: 855 DKYSRITKFKLA-------DRKPLTLHKHRPLAIKTMAPKFEENFNVDKKSYNPDMALQE 831

Query: 903 RRKLHKLIKREAKGAARELRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFK 950
            +KL   +K+E K A RE+RKD  F    K R++  +++   EK  R    +Q +E A K
Sbjct: 915 TQKLRAELKKEKKSALREIRKDAAFEAREKIRERREKDDAYHEKMARLVRSVQTEEGAEK 831

BLAST of MS001025 vs. ExPASy Swiss-Prot
Match: O43051 (Probable nucleolar complex protein 14 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nop14 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 4.0e-35
Identity = 230/936 (24.57%), Postives = 398/936 (42.52%), Query Frame = 0

Query: 5   SSLSSGNNDKKNQKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKGEERRI 64
           ++L +  N+KK++ +  +S   +   ++      N F+  +++RKFDV G++ KG E + 
Sbjct: 18  ANLGTRPNNKKSRTRSTESHEDRQAKVQKIQSDFNLFDRQFTKRKFDVGGRRVKGTEGKP 77

Query: 65  GLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERKLKLNK 124
           G++R    E R++T+  E ++  ++    D+R GE +  L   +K + R  RE++ + +K
Sbjct: 78  GVSRGVGEELRRRTIGAELKKRNRSGAIIDRRFGENNPHLSVEEKMLERFSREQQ-RRSK 137

Query: 125 SSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETRSTCKTFYQQKGGLL 184
              YNL D E+    G++ L  +   D FE+     D+     +       F    GG  
Sbjct: 138 RELYNL-DAEDVLTHGNRPLSDI---DSFEEPGFGLDEGEELNDEVVRRMHF----GGFE 197

Query: 185 EGD-------ETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSE 244
           + D       E   KSK+EVM EIIAKSK +KA++  +KE  E   E+LD++ E L    
Sbjct: 198 DSDAENEKEGEGAHKSKREVMSEIIAKSKHYKAERQAEKERYEDEREKLDEQMEDL--QS 257

Query: 245 ALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAMEI 304
            L     +S  + +KT  Q+ I ++   +                   +D  V+EM  + 
Sbjct: 258 FLSDYKKASRKSGIKT--QRPIISDGDAR-------------------YDSFVREMVFDK 317

Query: 305 RARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKFIS 364
           RA P++RTKT EE+AQ E +RL +LE++R  RM         +ED  +E  S++      
Sbjct: 318 RAHPTERTKTEEELAQIEADRLRELEDQRISRM------EHYQEDSASEAGSIE------ 377

Query: 365 GDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESE 424
                D    D+     KG   E  E  +    E+E                     ESE
Sbjct: 378 -----DEQATDNVFGFGKGLEQENEEEWNGINEEAE---------------------ESE 437

Query: 425 EDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAYK 484
           +++S++                 DT+  DD+     + + +E       + +EG +KA  
Sbjct: 438 DEESVNS----------------DTSFVDDE-----QLKVEEQPLVGSAIKNEGSEKA-- 497

Query: 485 SAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIV 544
                                         + Y    P S  +F  LL      D   +V
Sbjct: 498 -----------------------------SLAYTYPCPTSHVEFVQLLKGLDYKDYPTVV 557

Query: 545 GRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPF 604
            RIR  + ++L   N  +++ F  ILLQ+   L  +  +++ELL  L + L  ++ + P 
Sbjct: 558 SRIRTLHHVKLHPDNKSRLENFSVILLQHILHLTRQPMISMELLEHLTEHLHSLAQQFPS 617

Query: 605 YAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAIL 664
                    +    ++       PE   +P    L+   L   IFP SD  H+V++P +L
Sbjct: 618 ALGISFISVVEGMRKRLAKSYVYPE-IKFPEISDLLFFNLTGSIFPTSDKKHIVVSPVML 677

Query: 665 LMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSLSS 724
            M E L + P  +  D+    ++ +L L     S ++ PE I  +              S
Sbjct: 678 TMAESLSQSPADSLSDVCKKLYIANLFLKFQSYSHRYVPEVITAV--------------S 737

Query: 725 QNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDS----SFFSSDNFRAGL-- 784
           Q   + +   +  +     + + + E    N F I D++ D     S +  +    GL  
Sbjct: 738 QALYLLYPNFISIVPGTFALPDSLKE--KQNLFAIQDISLDEPQRLSLYELEELPTGLLQ 797

Query: 785 ---LLTVIGTLEGFVNVYGRLNSFPEIFSPILAILHELA-QQESMPDVLQDKFRNVAKVI 844
              L   +  +E  +++Y +  +F EIF PI+ +L   + ++E +   L +K  +  + +
Sbjct: 798 SSILFITLNLIEMAIDIYFKEQAFIEIFVPIMDMLQLYSLKKELLSKRLSEKLLSTLQAV 814

Query: 845 EAKTEEHYNGRQPLRMRKQKAVPIKLLNPKFEENF-VKGRDYDPDRERSERRKLHKLIKR 904
               E     R+PL ++  + + I    PKFEE + +    +D D ER++  KL    + 
Sbjct: 858 SDSIESAKANRKPLALQSHRPLGITSQVPKFEEGYSLDKSSHDIDPERAQLNKLRAQHRD 814

Query: 905 EAKGAARELRKDNHFLF-----EVKARDKALQEEER 918
             KGA R LRKD  F+      E +A+D+A  E+ R
Sbjct: 918 AKKGAIRTLRKDARFIARERRQEQRAKDQAYNEKMR 814

BLAST of MS001025 vs. ExPASy TrEMBL
Match: A0A6J1BRD4 (nucleolar protein 14 OS=Momordica charantia OX=3673 GN=LOC111005014 PE=3 SV=1)

HSP 1 Score: 1725.3 bits (4467), Expect = 0.0e+00
Identity = 932/952 (97.90%), Postives = 938/952 (98.53%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQ-KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKG 60
           MAKLSSLSSGNNDKKNQ KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKG
Sbjct: 1   MAKLSSLSSGNNDKKNQKKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRKG 60

Query: 61  EERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERK 120
           EERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERK
Sbjct: 61  EERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERK 120

Query: 121 LKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETRST--CKTFY 180
           LKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETR        Y
Sbjct: 121 LKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMPDDDDAAAAETRKVPFHDAQY 180

Query: 181 QQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSE 240
           QQKGGLLEG+ETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSE
Sbjct: 181 QQKGGLLEGEETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSE 240

Query: 241 ALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAMEI 300
           ALLSLTGSSNPNALKTLVQKSITNENPKKD LPAAAKTGNFNQEKPDAFDKLVKEMAMEI
Sbjct: 241 ALLSLTGSSNPNALKTLVQKSITNENPKKDNLPAAAKTGNFNQEKPDAFDKLVKEMAMEI 300

Query: 301 RARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKFIS 360
           RARPSDRTKTPEEIAQEER+RLEQLEEERQKRMLA+DGNSSDEEDEDAENASVQKRKFIS
Sbjct: 301 RARPSDRTKTPEEIAQEERDRLEQLEEERQKRMLASDGNSSDEEDEDAENASVQKRKFIS 360

Query: 361 GDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESE 420
           GDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESE
Sbjct: 361 GDDLGDSFTLDDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESE 420

Query: 421 EDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAYK 480
           EDDSIHGM+HSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKA+K
Sbjct: 421 EDDSIHGMKHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAFK 480

Query: 481 SAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIV 540
           +AAAKSSK TGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIV
Sbjct: 481 NAAAKSSKSTGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIV 540

Query: 541 GRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPF 600
           GRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPF
Sbjct: 541 GRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPF 600

Query: 601 YAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAIL 660
           YAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAIL
Sbjct: 601 YAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAIL 660

Query: 661 LMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSLSS 720
           LMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLV AGGRRSLSS
Sbjct: 661 LMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVVAGGRRSLSS 720

Query: 721 QNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTVIG 780
           QNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTVIG
Sbjct: 721 QNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTVIG 780

Query: 781 TLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEHYNG 840
           TLEGFVNVYGRLNSFPEIFSPIL ILHELAQQESMP VLQDKFRNVAKVIEAKTEEHYNG
Sbjct: 781 TLEGFVNVYGRLNSFPEIFSPILTILHELAQQESMPAVLQDKFRNVAKVIEAKTEEHYNG 840

Query: 841 RQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELRK 900
           RQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELRK
Sbjct: 841 RQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELRK 900

Query: 901 DNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           DNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK
Sbjct: 901 DNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 952

BLAST of MS001025 vs. ExPASy TrEMBL
Match: A0A6J1GJ18 (nucleolar protein 14 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454318 PE=3 SV=1)

HSP 1 Score: 1427.5 bits (3694), Expect = 0.0e+00
Identity = 797/955 (83.46%), Postives = 858/955 (89.84%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQ--KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRK 60
           MAKLS+LSS NNDKK++  KKKKKS+GPKAL+MKVSAPK NPFESIWSRRKFDVLGKKRK
Sbjct: 1   MAKLSNLSSSNNDKKDKKSKKKKKSAGPKALSMKVSAPKANPFESIWSRRKFDVLGKKRK 60

Query: 61  GEERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRER 120
           GEERRIGLARS AIEKRKKTLL+EYEQSGK+T+F+DKRIGEQDEELGEFDK ILRSQRER
Sbjct: 61  GEERRIGLARSIAIEKRKKTLLREYEQSGKSTEFSDKRIGEQDEELGEFDKAILRSQRER 120

Query: 121 KLKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP-DDDDAAAAETRSTC--KT 180
           KLKLNKSSK+NLSDGE+DDYFGS SLGAL A+DDFEDEV+P DDDDA AAET+       
Sbjct: 121 KLKLNKSSKFNLSDGEDDDYFGSNSLGALPANDDFEDEVIPDDDDDAEAAETKKGAYHGA 180

Query: 181 FYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQ 240
            Y QKGGLLEG+E KRKSKKEVMDEIIAKSKFFKAQKA+DKEENEQLIE+LDKKFESLVQ
Sbjct: 181 PYPQKGGLLEGEENKRKSKKEVMDEIIAKSKFFKAQKARDKEENEQLIEDLDKKFESLVQ 240

Query: 241 SEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAM 300
           SEALLSLT S N NALK LVQKSI NE+ KKD L A  KT NFNQEKPDAFDKLVKEMAM
Sbjct: 241 SEALLSLTSSGNANALKALVQKSIPNEHLKKDNLLATGKTENFNQEKPDAFDKLVKEMAM 300

Query: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKF 360
           EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLA D NSSD+ED DAENASVQK+KF
Sbjct: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLAPD-NSSDDEDGDAENASVQKQKF 360

Query: 361 ISGDDLGDSFTL-DDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDD 420
           ISGDDLGDSFTL DDER+HKKGWVDEI ERKDADGTESEDDDSA+DSDSS DD   DSDD
Sbjct: 361 ISGDDLGDSFTLDDDERNHKKGWVDEIFERKDADGTESEDDDSAEDSDSS-DDVGGDSDD 420

Query: 421 ESEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQK 480
           ESEEDD+  G +HSLKDWEQSDDDILD+N E+DDE+S  +KE DE+HPK          K
Sbjct: 421 ESEEDDNTRGRKHSLKDWEQSDDDILDSNSEEDDEASKEDKELDENHPK----------K 480

Query: 481 AYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDII 540
           A K A  KSSK  G SE+AKKLEK+TKR++K E+PYIIEAPESFDQF SLLADCS+SD+I
Sbjct: 481 ANKGAIVKSSKSEGSSEDAKKLEKNTKRENKPELPYIIEAPESFDQFLSLLADCSDSDVI 540

Query: 541 LIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSME 600
           LI+ RIRASNAIQL EKNLEKMQRFYGILLQYFAV ANKKPLNVELL+LL K LMEMS +
Sbjct: 541 LIIDRIRASNAIQLTEKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLLKSLMEMSRQ 600

Query: 601 IPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660
           IPFYAATCAR RISHTHQQFCV NKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP
Sbjct: 601 IPFYAATCARTRISHTHQQFCVDNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660

Query: 661 AILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRS 720
           AILLMCEYLMRCPIVTGRDIA+GSFLCSLLLYVA+QSLKFCPEAINFL+TLL+AA GRRS
Sbjct: 661 AILLMCEYLMRCPIVTGRDIAIGSFLCSLLLYVAKQSLKFCPEAINFLQTLLIAAAGRRS 720

Query: 721 LSSQNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLT 780
           L SQNPQIC+LVDL ALG LL IQNP NEITPL+FFF+M+LTE SS FSSD++RAG+LLT
Sbjct: 721 LPSQNPQICNLVDLHALGQLLRIQNPTNEITPLDFFFMMNLTEHSSIFSSDSYRAGVLLT 780

Query: 781 VIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEH 840
           VI TL+GFVNVYG+L SFPEIF P   ILHELA QE+MPDVL+DKFR VA+ IEAKTEEH
Sbjct: 781 VIETLDGFVNVYGQLKSFPEIFMPFSTILHELALQENMPDVLRDKFRKVAEAIEAKTEEH 840

Query: 841 YNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARE 900
           Y GRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRER E+RKL KL+KREAKGAARE
Sbjct: 841 YMGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERVEKRKLQKLLKREAKGAARE 900

Query: 901 LRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           LRKDNHFLF+VKARDKALQEEERAE+Y +A AFLQ+QEHAFKSGQL GKGRKRRK
Sbjct: 901 LRKDNHFLFDVKARDKALQEEERAERYRKASAFLQQQEHAFKSGQL-GKGRKRRK 942

BLAST of MS001025 vs. ExPASy TrEMBL
Match: A0A6J1KIP2 (nucleolar protein 14 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496098 PE=3 SV=1)

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 796/956 (83.26%), Postives = 857/956 (89.64%), Query Frame = 0

Query: 1   MAKLSSLSSGNN---DKKNQKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKR 60
           MAKLS+LSS NN   DKK++KKKKKS+GPKAL+MKVSAPK NPFESIWSRRKFDVLGKKR
Sbjct: 1   MAKLSNLSSSNNDKKDKKSKKKKKKSAGPKALSMKVSAPKANPFESIWSRRKFDVLGKKR 60

Query: 61  KGEERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRE 120
           KGEERRIGLARS AIEKRKKTLL+EYEQSGK+T+F+DKRIGEQDEELGEFDK ILRSQRE
Sbjct: 61  KGEERRIGLARSLAIEKRKKTLLREYEQSGKSTEFSDKRIGEQDEELGEFDKAILRSQRE 120

Query: 121 RKLKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP-DDDDAAAAETRSTC--K 180
           RKLKL+KSSK+NLSDGE+DDYFGS SLGAL A+DDF DEV+P DDDDA AAET+      
Sbjct: 121 RKLKLSKSSKFNLSDGEDDDYFGSNSLGALPANDDFVDEVIPDDDDDAEAAETKKGAYHG 180

Query: 181 TFYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLV 240
             YQQKGGLLEG+E KRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIE+LDKKFESLV
Sbjct: 181 APYQQKGGLLEGEENKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEDLDKKFESLV 240

Query: 241 QSEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMA 300
           QSEALLSLT S N NALK LVQKSI NE+ KKD L AA KT NFNQEKPDAFDKLVKEMA
Sbjct: 241 QSEALLSLTSSGNANALKALVQKSIPNEHSKKDNLLAAGKTENFNQEKPDAFDKLVKEMA 300

Query: 301 MEIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRK 360
           MEIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLA D NSSD+ED DAENASVQKRK
Sbjct: 301 MEIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLAPD-NSSDDEDGDAENASVQKRK 360

Query: 361 FISGDDLGDSFTL-DDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSD 420
           F+SGDDLGDSFTL DDER+HKKGWVDEI ERKDADGTESEDDDSA+D+DSS DD   DSD
Sbjct: 361 FVSGDDLGDSFTLDDDERNHKKGWVDEIFERKDADGTESEDDDSAEDTDSS-DDVGGDSD 420

Query: 421 DESEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQ 480
           DESEEDD  HG +HSLKDWEQSDDDILD+N E+DDE+S  +KE DE+HPK          
Sbjct: 421 DESEEDDKTHGRKHSLKDWEQSDDDILDSNSEEDDEASKEDKELDENHPK---------- 480

Query: 481 KAYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDI 540
           KA K A  KSSK  G SE+AKKLEK+TKR++K E+PYIIEAPESFDQF SLLADCS+SD+
Sbjct: 481 KANKGAIVKSSKSEGSSEDAKKLEKNTKRENKPELPYIIEAPESFDQFLSLLADCSDSDV 540

Query: 541 ILIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSM 600
           IL++ RIRASNAIQL EKNLEKMQRFYGILLQYFAV ANKKPLNVELL+LL KPLMEMS 
Sbjct: 541 ILVIDRIRASNAIQLTEKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLLKPLMEMSR 600

Query: 601 EIPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVIT 660
           +IPFYAATCAR RISHTHQQFCV NKNPENSSWPSSK LILLRLWSMIFPCSDYHH VIT
Sbjct: 601 QIPFYAATCARTRISHTHQQFCVDNKNPENSSWPSSKILILLRLWSMIFPCSDYHHAVIT 660

Query: 661 PAILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRR 720
           PAILLMCEYLMRCPIVTGRDIA+GSFLCSLLLYVA+QSLKFCPEAINFL TLL+AA GRR
Sbjct: 661 PAILLMCEYLMRCPIVTGRDIAIGSFLCSLLLYVAKQSLKFCPEAINFLLTLLIAAAGRR 720

Query: 721 SLSSQNPQICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLL 780
           SL SQNPQIC+LVDLQALG LL IQNP NEITPL+FFF+M+LTE SS FSSD++RAG+LL
Sbjct: 721 SLPSQNPQICNLVDLQALGQLLRIQNPTNEITPLDFFFMMNLTEHSSIFSSDSYRAGVLL 780

Query: 781 TVIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEE 840
           TVI TL+GFVNVYG+L SFPEIF P   ILHELA QE+MPDVL+DKFR VA+ IEAKTEE
Sbjct: 781 TVIETLDGFVNVYGQLKSFPEIFMPFSTILHELALQENMPDVLRDKFRKVAEAIEAKTEE 840

Query: 841 HYNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAAR 900
            Y GRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRER E+RKL KL+KREAKGAAR
Sbjct: 841 FYMGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERVEKRKLQKLLKREAKGAAR 900

Query: 901 ELRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           ELRKDNHFLF+VKARDKALQEEERAE+Y +A AFLQ+QEHAFKSGQL GKGRKRRK
Sbjct: 901 ELRKDNHFLFDVKARDKALQEEERAERYKKASAFLQQQEHAFKSGQL-GKGRKRRK 943

BLAST of MS001025 vs. ExPASy TrEMBL
Match: A0A6J1GHX5 (nucleolar protein 14 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454318 PE=3 SV=1)

HSP 1 Score: 1422.9 bits (3682), Expect = 0.0e+00
Identity = 797/956 (83.37%), Postives = 858/956 (89.75%), Query Frame = 0

Query: 1   MAKLSSLSSGNNDKKNQ--KKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKRK 60
           MAKLS+LSS NNDKK++  KKKKKS+GPKAL+MKVSAPK NPFESIWSRRKFDVLGKKRK
Sbjct: 1   MAKLSNLSSSNNDKKDKKSKKKKKSAGPKALSMKVSAPKANPFESIWSRRKFDVLGKKRK 60

Query: 61  GEERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRER 120
           GEERRIGLARS AIEKRKKTLL+EYEQSGK+T+F+DKRIGEQDEELGEFDK ILRSQRER
Sbjct: 61  GEERRIGLARSIAIEKRKKTLLREYEQSGKSTEFSDKRIGEQDEELGEFDKAILRSQRER 120

Query: 121 KLKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP-DDDDAAAAETRSTC--KT 180
           KLKLNKSSK+NLSDGE+DDYFGS SLGAL A+DDFEDEV+P DDDDA AAET+       
Sbjct: 121 KLKLNKSSKFNLSDGEDDDYFGSNSLGALPANDDFEDEVIPDDDDDAEAAETKKGAYHGA 180

Query: 181 FYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQ 240
            Y QKGGLLEG+E KRKSKKEVMDEIIAKSKFFKAQKA+DKEENEQLIE+LDKKFESLVQ
Sbjct: 181 PYPQKGGLLEGEENKRKSKKEVMDEIIAKSKFFKAQKARDKEENEQLIEDLDKKFESLVQ 240

Query: 241 SEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAM 300
           SEALLSLT S N NALK LVQKSI NE+ KKD L A  KT NFNQEKPDAFDKLVKEMAM
Sbjct: 241 SEALLSLTSSGNANALKALVQKSIPNEHLKKDNLLATGKTENFNQEKPDAFDKLVKEMAM 300

Query: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKF 360
           EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLA D NSSD+ED DAENASVQK+KF
Sbjct: 301 EIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLAPD-NSSDDEDGDAENASVQKQKF 360

Query: 361 ISGDDLGDSFTL-DDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDD 420
           ISGDDLGDSFTL DDER+HKKGWVDEI ERKDADGTESEDDDSA+DSDSS DD   DSDD
Sbjct: 361 ISGDDLGDSFTLDDDERNHKKGWVDEIFERKDADGTESEDDDSAEDSDSS-DDVGGDSDD 420

Query: 421 ESEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQK 480
           ESEEDD+  G +HSLKDWEQSDDDILD+N E+DDE+S  +KE DE+HPK          K
Sbjct: 421 ESEEDDNTRGRKHSLKDWEQSDDDILDSNSEEDDEASKEDKELDENHPK----------K 480

Query: 481 AYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDII 540
           A K A  KSSK  G SE+AKKLEK+TKR++K E+PYIIEAPESFDQF SLLADCS+SD+I
Sbjct: 481 ANKGAIVKSSKSEGSSEDAKKLEKNTKRENKPELPYIIEAPESFDQFLSLLADCSDSDVI 540

Query: 541 LIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSME 600
           LI+ RIRASNAIQL EKNLEKMQRFYGILLQYFAV ANKKPLNVELL+LL K LMEMS +
Sbjct: 541 LIIDRIRASNAIQLTEKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLLKSLMEMSRQ 600

Query: 601 IPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660
           IPFYAATCAR RISHTHQQFCV NKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP
Sbjct: 601 IPFYAATCARTRISHTHQQFCVDNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITP 660

Query: 661 AILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRS 720
           AILLMCEYLMRCPIVTGRDIA+GSFLCSLLLYVA+QSLKFCPEAINFL+TLL+AA GRRS
Sbjct: 661 AILLMCEYLMRCPIVTGRDIAIGSFLCSLLLYVAKQSLKFCPEAINFLQTLLIAAAGRRS 720

Query: 721 LSSQNP-QICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLL 780
           L SQNP QIC+LVDL ALG LL IQNP NEITPL+FFF+M+LTE SS FSSD++RAG+LL
Sbjct: 721 LPSQNPQQICNLVDLHALGQLLRIQNPTNEITPLDFFFMMNLTEHSSIFSSDSYRAGVLL 780

Query: 781 TVIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEE 840
           TVI TL+GFVNVYG+L SFPEIF P   ILHELA QE+MPDVL+DKFR VA+ IEAKTEE
Sbjct: 781 TVIETLDGFVNVYGQLKSFPEIFMPFSTILHELALQENMPDVLRDKFRKVAEAIEAKTEE 840

Query: 841 HYNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAAR 900
           HY GRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRER E+RKL KL+KREAKGAAR
Sbjct: 841 HYMGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERVEKRKLQKLLKREAKGAAR 900

Query: 901 ELRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           ELRKDNHFLF+VKARDKALQEEERAE+Y +A AFLQ+QEHAFKSGQL GKGRKRRK
Sbjct: 901 ELRKDNHFLFDVKARDKALQEEERAERYRKASAFLQQQEHAFKSGQL-GKGRKRRK 943

BLAST of MS001025 vs. ExPASy TrEMBL
Match: A0A6J1KKH7 (nucleolar protein 14 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496098 PE=3 SV=1)

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 796/957 (83.18%), Postives = 857/957 (89.55%), Query Frame = 0

Query: 1   MAKLSSLSSGNN---DKKNQKKKKKSSGPKALAMKVSAPKPNPFESIWSRRKFDVLGKKR 60
           MAKLS+LSS NN   DKK++KKKKKS+GPKAL+MKVSAPK NPFESIWSRRKFDVLGKKR
Sbjct: 1   MAKLSNLSSSNNDKKDKKSKKKKKKSAGPKALSMKVSAPKANPFESIWSRRKFDVLGKKR 60

Query: 61  KGEERRIGLARSHAIEKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRE 120
           KGEERRIGLARS AIEKRKKTLL+EYEQSGK+T+F+DKRIGEQDEELGEFDK ILRSQRE
Sbjct: 61  KGEERRIGLARSLAIEKRKKTLLREYEQSGKSTEFSDKRIGEQDEELGEFDKAILRSQRE 120

Query: 121 RKLKLNKSSKYNLSDGEEDDYFGSQSLGALTASDDFEDEVMP-DDDDAAAAETRSTC--K 180
           RKLKL+KSSK+NLSDGE+DDYFGS SLGAL A+DDF DEV+P DDDDA AAET+      
Sbjct: 121 RKLKLSKSSKFNLSDGEDDDYFGSNSLGALPANDDFVDEVIPDDDDDAEAAETKKGAYHG 180

Query: 181 TFYQQKGGLLEGDETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLV 240
             YQQKGGLLEG+E KRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIE+LDKKFESLV
Sbjct: 181 APYQQKGGLLEGEENKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEDLDKKFESLV 240

Query: 241 QSEALLSLTGSSNPNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMA 300
           QSEALLSLT S N NALK LVQKSI NE+ KKD L AA KT NFNQEKPDAFDKLVKEMA
Sbjct: 241 QSEALLSLTSSGNANALKALVQKSIPNEHSKKDNLLAAGKTENFNQEKPDAFDKLVKEMA 300

Query: 301 MEIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRK 360
           MEIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLA D NSSD+ED DAENASVQKRK
Sbjct: 301 MEIRARPSDRTKTPEEIAQEERERLEQLEEERQKRMLAPD-NSSDDEDGDAENASVQKRK 360

Query: 361 FISGDDLGDSFTL-DDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSD 420
           F+SGDDLGDSFTL DDER+HKKGWVDEI ERKDADGTESEDDDSA+D+DSS DD   DSD
Sbjct: 361 FVSGDDLGDSFTLDDDERNHKKGWVDEIFERKDADGTESEDDDSAEDTDSS-DDVGGDSD 420

Query: 421 DESEEDDSIHGMEHSLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQ 480
           DESEEDD  HG +HSLKDWEQSDDDILD+N E+DDE+S  +KE DE+HPK          
Sbjct: 421 DESEEDDKTHGRKHSLKDWEQSDDDILDSNSEEDDEASKEDKELDENHPK---------- 480

Query: 481 KAYKSAAAKSSKITGHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDI 540
           KA K A  KSSK  G SE+AKKLEK+TKR++K E+PYIIEAPESFDQF SLLADCS+SD+
Sbjct: 481 KANKGAIVKSSKSEGSSEDAKKLEKNTKRENKPELPYIIEAPESFDQFLSLLADCSDSDV 540

Query: 541 ILIVGRIRASNAIQLKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSM 600
           IL++ RIRASNAIQL EKNLEKMQRFYGILLQYFAV ANKKPLNVELL+LL KPLMEMS 
Sbjct: 541 ILVIDRIRASNAIQLTEKNLEKMQRFYGILLQYFAVSANKKPLNVELLNLLLKPLMEMSR 600

Query: 601 EIPFYAATCARMRISHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVIT 660
           +IPFYAATCAR RISHTHQQFCV NKNPENSSWPSSK LILLRLWSMIFPCSDYHH VIT
Sbjct: 601 QIPFYAATCARTRISHTHQQFCVDNKNPENSSWPSSKILILLRLWSMIFPCSDYHHAVIT 660

Query: 661 PAILLMCEYLMRCPIVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRR 720
           PAILLMCEYLMRCPIVTGRDIA+GSFLCSLLLYVA+QSLKFCPEAINFL TLL+AA GRR
Sbjct: 661 PAILLMCEYLMRCPIVTGRDIAIGSFLCSLLLYVAKQSLKFCPEAINFLLTLLIAAAGRR 720

Query: 721 SLSSQNP-QICHLVDLQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLL 780
           SL SQNP QIC+LVDLQALG LL IQNP NEITPL+FFF+M+LTE SS FSSD++RAG+L
Sbjct: 721 SLPSQNPQQICNLVDLQALGQLLRIQNPTNEITPLDFFFMMNLTEHSSIFSSDSYRAGVL 780

Query: 781 LTVIGTLEGFVNVYGRLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTE 840
           LTVI TL+GFVNVYG+L SFPEIF P   ILHELA QE+MPDVL+DKFR VA+ IEAKTE
Sbjct: 781 LTVIETLDGFVNVYGQLKSFPEIFMPFSTILHELALQENMPDVLRDKFRKVAEAIEAKTE 840

Query: 841 EHYNGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAA 900
           E Y GRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRER E+RKL KL+KREAKGAA
Sbjct: 841 EFYMGRQPLRMRKQKAVPIKLLNPKFEENFVKGRDYDPDRERVEKRKLQKLLKREAKGAA 900

Query: 901 RELRKDNHFLFEVKARDKALQEEERAEKYGRARAFLQEQEHAFKSGQLGGKGRKRRK 950
           RELRKDNHFLF+VKARDKALQEEERAE+Y +A AFLQ+QEHAFKSGQL GKGRKRRK
Sbjct: 901 RELRKDNHFLFDVKARDKALQEEERAERYKKASAFLQQQEHAFKSGQL-GKGRKRRK 944

BLAST of MS001025 vs. TAIR 10
Match: AT1G69070.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Nop14-like protein (InterPro:IPR007276); Has 69842 Blast hits to 35213 proteins in 1572 species: Archae - 363; Bacteria - 20593; Metazoa - 20851; Fungi - 8010; Plants - 2912; Viruses - 517; Other Eukaryotes - 16596 (source: NCBI BLink). )

HSP 1 Score: 813.9 bits (2101), Expect = 1.3e-235
Identity = 493/943 (52.28%), Postives = 666/943 (70.63%), Query Frame = 0

Query: 14  KKNQKKKKKSSGPKALAMKVSAPK-PNPFESIWSRRKFDVLGKKRKGEERRIGLARSHAI 73
           KK  KK ++  GP A+AMK    K  NPFESI SRRKFD+LGKKRKGEER + ++R+ A+
Sbjct: 9   KKPNKKGERRMGPDAVAMKAKTQKVDNPFESIRSRRKFDILGKKRKGEERFVSVSRTRAV 68

Query: 74  EKRKKTLLKEYEQSGKATKFTDKRIGEQDEELGEFDKGILRSQRERKLKLNKSSKYNLSD 133
           +KRK TL KEYEQS K++ F DKRIGEQ++ELGEFDKGI+RSQR+R+LKL K S YNLSD
Sbjct: 69  DKRKNTLEKEYEQSLKSSVFLDKRIGEQNDELGEFDKGIIRSQRQRQLKLAKKSMYNLSD 128

Query: 134 GEEDDYFGSQSLGALTASDDFE-----DEVMPDDDDAAAAETRSTCKTFYQQKGGLLEGD 193
           GEED Y    +LG  +  DDF+     DE + DDD  A+A  R   K   + +     G+
Sbjct: 129 GEEDVY-EDGALGGSSVKDDFDSGLLSDEDLQDDDLEASASKR--LKHLNRNREVDASGE 188

Query: 194 ETKRKSKKEVMDEIIAKSKFFKAQKAKDKEENEQLIEELDKKFESLVQSEALLSLTGSSN 253
           E +RKSKKEVM+EII KSK  + +KAK KEE  +L++ELDK F+SLV SEA+ SLT    
Sbjct: 189 EERRKSKKEVMEEIIMKSKLGRMEKAKQKEEKGKLMDELDKNFKSLVNSEAMESLT---- 248

Query: 254 PNALKTLVQKSITNENPKKDYLPAAAKTGNFNQEKPDAFDKLVKEMAMEIRARPSDRTKT 313
                   +  +  EN +  YL +                  + +M+MEIRARPS+RTKT
Sbjct: 249 --------KPFVAEENTRDPYLLS------------------LNDMSMEIRARPSERTKT 308

Query: 314 PEEIAQEERERLEQLEEERQKRMLATDGNSSDEEDEDAENASVQKRKFISGDDLGDSFTL 373
           PEEIAQ+ERE+LE LEEER+KRM  T+   SD ++E     S ++   ISGDDLGDSF++
Sbjct: 309 PEEIAQKEREKLEALEEERKKRMQETE-ELSDGDEEIGGEESTKRLTVISGDDLGDSFSV 368

Query: 374 DDERSHKKGWVDEILERKDADGTESEDDDSADDSDSSHDDRDEDSDDESEEDDSIHGMEH 433
           ++++  K+GW+D++LER+D     + D+  +D+ + S  + +ED D ES+  D      H
Sbjct: 369 EEDKP-KRGWIDDVLERED-----NVDNSESDEDEDSESEEEEDDDGESDGGDEKQRKGH 428

Query: 434 SLKDWEQSDDDILDTNLEDDDESSGGEKEPDEDHPKEEEVDHEGPQKAYKSAAAKSSKIT 493
            L+DWEQSDD+ L   LED++E    + E D+D    E   H+  +  Y +A  K   ++
Sbjct: 429 HLEDWEQSDDE-LGAELEDEEED---DDEEDDDEEDAELRVHKKLKNDY-AAPYKGEGLS 488

Query: 494 GHSEEAKKLEKDTKRKSKEEIPYIIEAPESFDQFSSLLADCSNSDIILIVGRIRASNAIQ 553
           G  +E   ++K     ++ +IP++I+ P++F++  +L+ DCSN D+ILIV RIR +++I+
Sbjct: 489 GTVKEKTNMKK--MSSTQRDIPFMIDPPKNFEELLALVEDCSNEDVILIVNRIRIAHSIK 548

Query: 554 LKEKNLEKMQRFYGILLQYFAVLANKKPLNVELLSLLFKPLMEMSMEIPFYAATCARMRI 613
           +K +N +KMQ FYG+LLQYFAVLA+KKPLN +LL++L KPL+EMSMEIP++AA CAR R+
Sbjct: 549 IKAENRKKMQVFYGVLLQYFAVLASKKPLNFDLLNMLVKPLIEMSMEIPYFAAICARQRL 608

Query: 614 SHTHQQFCVHNKNPENSSWPSSKTLILLRLWSMIFPCSDYHHVVITPAILLMCEYLMRCP 673
             T  QFC   KNPE+  WPS KTL LLRLWS+IFPCSD+ H V+TP+ILLMCEYLMRCP
Sbjct: 609 LKTRSQFCEAIKNPEDGCWPSLKTLFLLRLWSLIFPCSDFRHAVMTPSILLMCEYLMRCP 668

Query: 674 IVTGRDIAVGSFLCSLLLYVARQSLKFCPEAINFLRTLLVAAGGRRSLSSQNPQICHLVD 733
           I +GRDIA+GSFLCS++L    QS KFCPEAI F+RTLL+AA  ++S +S   +  H ++
Sbjct: 669 ISSGRDIAIGSFLCSIVLL---QSKKFCPEAILFIRTLLMAASDKKSPASAESEFYHFME 728

Query: 734 LQALGPLLHIQNPINEITPLNFFFIMDLTEDSSFFSSDNFRAGLLLTVIGTLEGFVNVYG 793
           L++L PLL IQ+ + E+ PLNF  IM+   DS +FSSD+FRA +L +V+ TLEGFV + G
Sbjct: 729 LKSLTPLLCIQDNVKEVMPLNFLKIMNEPADSPYFSSDDFRASILSSVVETLEGFVEING 788

Query: 794 RLNSFPEIFSPILAILHELAQQESMPDVLQDKFRNVAKVIEAKTEEHYNGRQPLRMRKQK 853
            L+SFPEIF PI  +LH++  QE +P  L++K  +VAK+IE KT++H+  R+PL MRK K
Sbjct: 789 GLSSFPEIFMPISTLLHQIGNQEKIPQTLKEKLEDVAKLIEKKTDDHHKERKPLSMRKHK 848

Query: 854 AVPIKLLNPKFEENFVKGRDYDPDRERSERRKLHKLIKREAKGAARELRKDNHFLFEVKA 913
            V I+++NPKFEENFV G D DPD+ RS+ +KL + +KREA+GA RELRKD++F+  VKA
Sbjct: 849 PVAIRMVNPKFEENFVPGMDNDPDKYRSDLKKLKRKLKREARGAVRELRKDSYFMSTVKA 901

Query: 914 RDKALQEEERAEKYGRARAFLQEQEHAFKSGQLG-GKGRKRRK 950
           ++KA  E+ERAEK+G+A AFLQEQEHAFKSGQLG GKG+KRR+
Sbjct: 909 KEKAAHEQERAEKHGKAWAFLQEQEHAFKSGQLGKGKGKKRRR 901