MS001018 (gene) Bitter gourd (TR) v1

Overview


Name	MS001018
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	cytochrome b5
Location	scaffold36: 875396 .. 877140 (+)
RNA-Seq Expression	MS001018
Synteny	MS001018

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAGCATCTCGAACCTATATTCGATTCAAGAAATCTCACACCACAGTTCCAACGACGACTGTTGGATTGTTATCGATGGCAAGGTACGTCCCTCGCTAATATTCGTCGTCCTATATCAGTTCTGTTCTTCGTCCTCTTTATTTGTTTGCTGTATGATTCGTGAGCAATTTACTCTCTGGGTCTTTTTGGGTTATGCTCGTTTCAGTGGTATTTGTGATTCTACGTGATGCAATCTGATTCTGGAGGGAATTGTTGTGAAAGAGTTCGCTAACTTCAATCATCAGATTAATCTAATTTAACTTTTATGAATCCAATTTGTATTGTTGGTCGACTTTTTAGGGTGGATGATGGGGGAATGGGGTGGGGATGTGATAGTTTGACTCGCATTAGCATTCGGCTGATGCTGAAATTGGGCTAAGTTTGTCACTGTGATGATGGGAAAATAATTAGACAGTCTTATTGTTTGATGGTATTTGTGGGTTATCTGGAATTTAGGCAACTGGTTATTATGATAGTTATAATTGTAATTGATAAGGATTAAGACATTAATCTAATTAAGTCCTGTTTAGGATTAAGCAATTATATTATCAATATAGGTCTGACTTATAAACTTTTTTTAAGAGTTTTTTTCCTAGTACAACATTAGAATTTGTTTAAGAGTGGATAACTGAGACAGTTTAATGGCTTGGCTTTTGTATGTTATGGGGGGAGAGTGGAGATAACTGGATAGGAAATATGAGAAGAAAAATGGAGGATGGCGTAGAGAACCCAAGCATACGGCTCACACACGGATCAATAGATTTAATGCTCAATTAATTCTCACGTCGAAGTTCTAGAAATTACAATGAAAAAGTTAGGGGTTCTAGTATGAATTCAAAGAAGACTAAAAGTTTCAATATCATATTAGAACTTTCTATACTGGTGTTTGAATCTTTGAACTTGTTGGATGTGACGATTTCAATGATTTGTTGCTTTGAAATTCTTGAGATTATATTGATGTTTAAGTTGCATCTTTGAGACTATGGTCTATTTCCACAATCTTTCCGAAATTTTTATACTCTTTTTCTTTATATTTCTCTTCGTAGATTTTCAATCATGTCATCTATTTTGTCAAAAGTTTGAAAATTGTTTTCATAAAGGTTTTAGAGTTTGGAAAGGAGTAGTTGTTTTTTGTCACTCGTATTTTTTCTAGCTCAGCATGGTCCATTGTTTTGACATTATAATAAAATTCAGGTCCATTACTCAATTCATCAACAATAATATTTGCCTAACCATCATGTTTGTTATCTTCTACCCTATTTTCTTTTGAGACGATGCATTCCAACATTTCTTTAAAATGACGTTCTATCATTTTTTAGGTATACGATTTGACGTCATATTTGGACGAGCATCCTGGTGGAGATGATGTTATTCTTGCAGCAACTGGTATTCTTGTGCTTTCTTATGGATTATTCCCTCCTAAAAATTTGAACACTGATAGACTTATTTTTTTCCCATCAAAGGCAGAGATGCCACAGATGACTTTGAAGATGCCGGTCATAGCAAAGACGCAAGAGAACTGATGCAGAAGTTCTATATTGGTGAGCTTGATGCATCTTCTTCAGCAATCCCAGAACTCAAAACGGAGCAACTCGACGATTATGCAGCTCGGGTTCAGGGCTTGGCAAAGCAATACTGGGCTGCTCCTGTTGCAATGCTTGGTATTTCTGTGGTAATTGGCTGGTTATTCTTGCGCAAAAAA

mRNA sequence

ATGCCGAGCATCTCGAACCTATATTCGATTCAAGAAATCTCACACCACAGTTCCAACGACGACTGTTGGATTGTTATCGATGGCAAGGTATACGATTTGACGTCATATTTGGACGAGCATCCTGGTGGAGATGATGTTATTCTTGCAGCAACTGGCAGAGATGCCACAGATGACTTTGAAGATGCCGGTCATAGCAAAGACGCAAGAGAACTGATGCAGAAGTTCTATATTGGTGAGCTTGATGCATCTTCTTCAGCAATCCCAGAACTCAAAACGGAGCAACTCGACGATTATGCAGCTCGGGTTCAGGGCTTGGCAAAGCAATACTGGGCTGCTCCTGTTGCAATGCTTGGTATTTCTGTGGTAATTGGCTGGTTATTCTTGCGCAAAAAA

Coding sequence (CDS)

ATGCCGAGCATCTCGAACCTATATTCGATTCAAGAAATCTCACACCACAGTTCCAACGACGACTGTTGGATTGTTATCGATGGCAAGGTATACGATTTGACGTCATATTTGGACGAGCATCCTGGTGGAGATGATGTTATTCTTGCAGCAACTGGCAGAGATGCCACAGATGACTTTGAAGATGCCGGTCATAGCAAAGACGCAAGAGAACTGATGCAGAAGTTCTATATTGGTGAGCTTGATGCATCTTCTTCAGCAATCCCAGAACTCAAAACGGAGCAACTCGACGATTATGCAGCTCGGGTTCAGGGCTTGGCAAAGCAATACTGGGCTGCTCCTGTTGCAATGCTTGGTATTTCTGTGGTAATTGGCTGGTTATTCTTGCGCAAAAAA

Protein sequence

MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFEDAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGISVVIGWLFLRKK

Homology

[Close]

BLAST of MS001018 vs. NCBI nr
Match: XP_022131367.1 (cytochrome b5 [Momordica charantia])

HSP 1 Score: 265.4 bits (677), Expect = 2.6e-67
Identity = 130/131 (99.24%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE
Sbjct: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS
Sbjct: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120

Query: 121 VVIGWLFLRKK 132
           VVIGWLFLR K
Sbjct: 121 VVIGWLFLRNK 131

[Close]

BLAST of MS001018 vs. NCBI nr
Match: KAG6598642.1 (Cytochrome b5 isoform A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 231.1 bits (588), Expect = 5.5e-57
Identity = 109/131 (83.21%), Postives = 121/131 (92.37%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSI+E+S HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELM+KFYIGELD SSSAIPEL+T++ D YAARVQ L KQYW  P+A+LGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGIS 120

Query: 121 VVIGWLFLRKK 132
           VV+GWL+L KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of MS001018 vs. NCBI nr
Match: XP_022961879.1 (cytochrome b5 [Cucurbita moschata])

HSP 1 Score: 228.8 bits (582), Expect = 2.7e-56
Identity = 108/131 (82.44%), Postives = 120/131 (91.60%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSI+E+S HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELM+KFYIGELD SSSAIPEL+ ++ D YAARVQ L KQYW  P+A+LGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELEAKEPDGYAARVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVIGWLFLRKK 132
           VV+GWL+L KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of MS001018 vs. NCBI nr
Match: XP_023545611.1 (cytochrome b5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 228.4 bits (581), Expect = 3.6e-56
Identity = 107/131 (81.68%), Postives = 120/131 (91.60%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSI+E+S HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELM+KFYIGELD SSSAIPEL+T++ D YAARVQ L KQYW  P+A+LG+S
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGMS 120

Query: 121 VVIGWLFLRKK 132
           V +GWL+L KK
Sbjct: 121 VALGWLYLLKK 131

[Close]

BLAST of MS001018 vs. NCBI nr
Match: XP_022997294.1 (cytochrome b5 [Cucurbita maxima])

HSP 1 Score: 225.7 bits (574), Expect = 2.3e-55
Identity = 107/131 (81.68%), Postives = 119/131 (90.84%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSI+EIS HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEISRHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIIAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKD RELM+KFYIGELD SSSAIP L+T++ D YAA+VQ L KQYW  P+A+LGIS
Sbjct: 61  DAGHSKDTRELMKKFYIGELDPSSSAIPALETKEPDGYAAQVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVIGWLFLRKK 132
           VV+GWL+L KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of MS001018 vs. ExPASy Swiss-Prot
Match: Q9FDW8 (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.0e-41
Identity = 81/134 (60.45%), Postives = 104/134 (77.61%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MP+++ LYS++E + H+  DDCW+VIDGKVYD++SY+DEHPGGDDV+LA  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASS-SAIPEL---KTEQLDDYAARVQGLAKQYWAAPVAM 120
           DAGHSKDARELM+K++IGELD SS   IPEL   K +Q  D   ++  L KQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LGISVVIGWLFLRK 131
           + ISV +  LF RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of MS001018 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-26
Identity = 57/134 (42.54%), Postives = 87/134 (64.93%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           M S   +   +E+S H+   DCW++I GKVYD+T ++D+HPGGD+V+L++TG+DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQ-----------Y 120
           D GHS  AR++M+K+YIGE+D  SS +P  +T     Y A VQ    Q            
Sbjct: 61  DVGHSDTARDMMEKYYIGEID--SSTVPATRT-----YVAPVQPAYNQDKTPEFMIKILQ 120

Query: 121 WAAPVAMLGISVVI 124
           +  P+ +LG+++V+
Sbjct: 121 FLVPILILGLALVV 127

[Close]

BLAST of MS001018 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 4.0e-26
Identity = 54/129 (41.86%), Postives = 87/129 (67.44%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           M S   + S +E+S H+   DCW++I GKVYD+T ++D+HPGGD+V+L++TG+DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQY------WAAPV 120
           D GHS  AR++M K++IGE+D  SS++P  +T       A  Q    ++      +  P+
Sbjct: 61  DVGHSDTARDMMDKYFIGEID--SSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPI 120

Query: 121 AMLGISVVI 124
            +LG+++V+
Sbjct: 121 LILGLALVV 127

[Close]

BLAST of MS001018 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 4.4e-25
Identity = 44/80 (55.00%), Postives = 70/80 (87.50%), Query Frame = 0


Query: 5  SNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFEDAGH 64
          + ++++ E+S H+   DCWIVI+GKVY++T +L++HPGGDDV+L++TG+DATDDFED GH
Sbjct: 5  AKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 64

Query: 65 SKDARELMQKFYIGELDASS 85
          S+ ARE+M+++Y+GE+D ++
Sbjct: 65 SESAREMMEQYYVGEIDPTT 84

[Close]

BLAST of MS001018 vs. ExPASy Swiss-Prot
Match: Q9ZWT2 (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 5.8e-25
Identity = 45/78 (57.69%), Postives = 66/78 (84.62%), Query Frame = 0


Query: 7  LYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFEDAGHSK 66
          ++++ E+S HSS  DCWIVIDGKVYD+T +LD+HPGGD+VIL +TG+DATDDFED GHS 
Sbjct: 7  VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 67 DARELMQKFYIGELDASS 85
           A+ ++ ++Y+G++D ++
Sbjct: 67 TAKAMLDEYYVGDIDTAT 84

[Close]

BLAST of MS001018 vs. ExPASy TrEMBL
Match: A0A6J1BQT8 (cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111004609 PE=3 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.3e-67
Identity = 130/131 (99.24%), Postives = 130/131 (99.24%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE
Sbjct: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS
Sbjct: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120

Query: 121 VVIGWLFLRKK 132
           VVIGWLFLR K
Sbjct: 121 VVIGWLFLRNK 131

[Close]

BLAST of MS001018 vs. ExPASy TrEMBL
Match: A0A6J1HFA7 (cytochrome b5 OS=Cucurbita moschata OX=3662 GN=LOC111462520 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.3e-56
Identity = 108/131 (82.44%), Postives = 120/131 (91.60%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSI+E+S HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELM+KFYIGELD SSSAIPEL+ ++ D YAARVQ L KQYW  P+A+LGIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELEAKEPDGYAARVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVIGWLFLRKK 132
           VV+GWL+L KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of MS001018 vs. ExPASy TrEMBL
Match: A0A6J1KB15 (cytochrome b5 OS=Cucurbita maxima OX=3661 GN=LOC111492240 PE=3 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.1e-55
Identity = 107/131 (81.68%), Postives = 119/131 (90.84%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSI+EIS HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEISRHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIIAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKD RELM+KFYIGELD SSSAIP L+T++ D YAA+VQ L KQYW  P+A+LGIS
Sbjct: 61  DAGHSKDTRELMKKFYIGELDPSSSAIPALETKEPDGYAAQVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVIGWLFLRKK 132
           VV+GWL+L KK
Sbjct: 121 VVLGWLYLLKK 131

[Close]

BLAST of MS001018 vs. ExPASy TrEMBL
Match: A0A0A0KIR1 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G366540 PE=3 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 8.0e-54
Identity = 105/131 (80.15%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSIQE+S HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELM+KFYIG LD SS    +L+T Q+D YA RVQ L KQYW APVA++GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVIGWLFLRKK 132
           VV+GW +LRKK
Sbjct: 121 VVLGWAYLRKK 131

[Close]

BLAST of MS001018 vs. ExPASy TrEMBL
Match: A0A1S3BCR4 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.3e-53
Identity = 102/131 (77.86%), Postives = 117/131 (89.31%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MPSIS LYSIQE+S HSS+DDCWI+IDGKVYDLTSYLDEHPGGDD+I+ ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQYWAAPVAMLGIS 120
           DAGHSKDARELM+KFYIG LD SSS   +L+T Q+D YA RVQ L KQYW APVA++GIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVIGWLFLRKK 132
           +V+GW++LRKK
Sbjct: 121 MVLGWVYLRKK 131

[Close]

BLAST of MS001018 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 169.9 bits (429), Expect = 1.4e-42
Identity = 81/134 (60.45%), Postives = 104/134 (77.61%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           MP+++ LYS++E + H+  DDCW+VIDGKVYD++SY+DEHPGGDDV+LA  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASS-SAIPEL---KTEQLDDYAARVQGLAKQYWAAPVAM 120
           DAGHSKDARELM+K++IGELD SS   IPEL   K +Q  D   ++  L KQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 LGISVVIGWLFLRK 131
           + ISV +  LF RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of MS001018 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 119.0 bits (297), Expect = 2.8e-27
Identity = 54/129 (41.86%), Postives = 87/129 (67.44%), Query Frame = 0


Query: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFE 60
           M S   + S +E+S H+   DCW++I GKVYD+T ++D+HPGGD+V+L++TG+DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMQKFYIGELDASSSAIPELKTEQLDDYAARVQGLAKQY------WAAPV 120
           D GHS  AR++M K++IGE+D  SS++P  +T       A  Q    ++      +  P+
Sbjct: 61  DVGHSDTARDMMDKYFIGEID--SSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPI 120

Query: 121 AMLGISVVI 124
            +LG+++V+
Sbjct: 121 LILGLALVV 127

[Close]

BLAST of MS001018 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 115.5 bits (288), Expect = 3.1e-26
Identity = 44/80 (55.00%), Postives = 70/80 (87.50%), Query Frame = 0


Query: 5  SNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFEDAGH 64
          + ++++ E+S H+   DCWIVI+GKVY++T +L++HPGGDDV+L++TG+DATDDFED GH
Sbjct: 5  AKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 64

Query: 65 SKDARELMQKFYIGELDASS 85
          S+ ARE+M+++Y+GE+D ++
Sbjct: 65 SESAREMMEQYYVGEIDPTT 84

[Close]

BLAST of MS001018 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 115.2 bits (287), Expect = 4.1e-26
Identity = 45/78 (57.69%), Postives = 66/78 (84.62%), Query Frame = 0


Query: 7  LYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFEDAGHSK 66
          ++++ E+S HSS  DCWIVIDGKVYD+T +LD+HPGGD+VIL +TG+DATDDFED GHS 
Sbjct: 7  VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 67 DARELMQKFYIGELDASS 85
           A+ ++ ++Y+G++D ++
Sbjct: 67 TAKAMLDEYYVGDIDTAT 84

[Close]

BLAST of MS001018 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 110.2 bits (274), Expect = 1.3e-24
Identity = 51/126 (40.48%), Postives = 81/126 (64.29%), Query Frame = 0


Query: 4   ISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAATGRDATDDFEDAG 63
           ++NL S  +++ H   +DCWI+I GKVYD+++++DEHPGGD+V+LA TG+DA+ DFED  
Sbjct: 1   MANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVN 60

Query: 64  HSKDARELMQKFYIGELDASSSAI------PELKTEQLDDYAARVQGLAKQYWAAPVAML 123
           HSKDA+ELM+K+ IG++D S+  +      P  K     +      G     +  P+ +L
Sbjct: 61  HSKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLIL 120

The following BLAST results are available for this feature:

BLAST of MS001018 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131367.1	2.6e-67	99.24	cytochrome b5 [Momordica charantia]	[more]
KAG6598642.1	5.5e-57	83.21	Cytochrome b5 isoform A, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022961879.1	2.7e-56	82.44	cytochrome b5 [Cucurbita moschata]	[more]
XP_023545611.1	3.6e-56	81.68	cytochrome b5 [Cucurbita pepo subsp. pepo]	[more]
XP_022997294.1	2.3e-55	81.68	cytochrome b5 [Cucurbita maxima]	[more]

BLAST of MS001018 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FDW8	2.0e-41	60.45	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
P40934	2.3e-26	42.54	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
Q42342	4.0e-26	41.86	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
O48845	4.4e-25	55.00	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
Q9ZWT2	5.8e-25	57.69	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]

BLAST of MS001018 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQT8	1.3e-67	99.24	cytochrome b5 OS=Momordica charantia OX=3673 GN=LOC111004609 PE=3 SV=1	[more]
A0A6J1HFA7	1.3e-56	82.44	cytochrome b5 OS=Cucurbita moschata OX=3662 GN=LOC111462520 PE=3 SV=1	[more]
A0A6J1KB15	1.1e-55	81.68	cytochrome b5 OS=Cucurbita maxima OX=3661 GN=LOC111492240 PE=3 SV=1	[more]
A0A0A0KIR1	8.0e-54	80.15	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3BCR4	2.3e-53	77.86	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1	[more]

BLAST of MS001018 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26340.1	1.4e-42	60.45	cytochrome B5 isoform A	[more]
AT5G53560.1	2.8e-27	41.86	cytochrome B5 isoform E	[more]
AT2G32720.1	3.1e-26	55.00	cytochrome B5 isoform B	[more]
AT5G48810.1	4.1e-26	57.69	cytochrome B5 isoform D	[more]
AT2G46650.1	1.3e-24	40.48	cytochrome B5 isoform C	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 68..80 score: 45.45 coord: 30..40 score: 55.65 coord: 40..54 score: 55.35 coord: 55..62 score: 44.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 2.0E-30 score: 117.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 1.3E-25 score: 89.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 29.011522
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 3..86 e-value: 1.1E-33 score: 117.1
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 4..85
None	No IPR available	PANTHER	PTHR19359:SF25	CYTOCHROME B5 ISOFORM A	coord: 3..130
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 3..130
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS001018.1	MS001018.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding