MS001003 (gene) Bitter gourd (TR) v1

BLAST of MS001003 vs. NCBI nr
Match: XP_022132312.1 (COBW domain-containing protein 1 isoform X1 [Momordica charantia])

HSP 1 Score: 702.6 bits (1812), Expect = 1.9e-198
Identity = 362/396 (91.41%), Postives = 364/396 (91.92%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESTVKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNL+FQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY
Sbjct: 181 SIITVVDAKNLNFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCISDDNKVDLDK VHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISDDNKVDLDK-VHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
           PTRKWNNGES MNKIVFIGRNLNEDVL ET RACAV
Sbjct: 361 PTRKWNNGESPMNKIVFIGRNLNEDVLRETXRACAV 367

BLAST of MS001003 vs. NCBI nr
Match: KAA0064757.1 (COBW domain-containing protein 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 662.1 bits (1707), Expect = 2.9e-186
Identity = 339/395 (85.82%), Postives = 353/395 (89.37%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGET+TA   HED GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETVTATQQHEDGGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES++KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAFHQI FADTI+LNKVDLVSS   G GALEDLEY
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIVFADTIILNKVDLVSSDR-GDGALEDLEY 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLIL+CN+YNA NT HLEALLKESRSLST+DLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILDCNSYNAANTAHLEALLKESRSLSTQDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS+ +KVDLDKVVHSWLEEILW+KKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISEHHKVDLDKVVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACA 396
           PTR+W+NGES MNKIVFIGRNLNEDVLS+TFR CA
Sbjct: 361 PTRQWSNGESQMNKIVFIGRNLNEDVLSKTFRECA 366

BLAST of MS001003 vs. NCBI nr
Match: XP_023546079.1 (COBW domain-containing protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 659.1 bits (1699), Expect = 2.5e-185
Identity = 342/396 (86.36%), Postives = 355/396 (89.65%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGETITAA  HED+GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES+VKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIAFADTI+LNKVDLV+S+  G GALEDLE+
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSER-GDGALEDLEF 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLILNCNAYNA NT HLE LLKESRSLST+DLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS  +KVDLDK V SWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRE+YE+V
Sbjct: 301 LCISYHDKVDLDK-VRSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVREIYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
           PTR+WNNGES MNKIVFIGRNL+EDVLSETFRACAV
Sbjct: 361 PTRQWNNGESQMNKIVFIGRNLSEDVLSETFRACAV 366

BLAST of MS001003 vs. NCBI nr
Match: XP_022962150.1 (COBW domain-containing protein 1 [Cucurbita moschata])

HSP 1 Score: 658.7 bits (1698), Expect = 3.2e-185
Identity = 342/396 (86.36%), Postives = 355/396 (89.65%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGETITAA  HED+GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES+VKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIAFADTI+LNKVDLV+S+  G GALEDLE+
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSER-GDGALEDLEF 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLILNCNAYNA NT HLE LLKESRSLST+DLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS  +KVDLDK V SWLEEILWD+KGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISYHDKVDLDK-VRSWLEEILWDRKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
           PTR+WNNGES MNKIVFIGRNL+EDVLSETFRACAV
Sbjct: 361 PTRQWNNGESQMNKIVFIGRNLSEDVLSETFRACAV 366

BLAST of MS001003 vs. NCBI nr
Match: KAG6598632.1 (COBW domain-containing protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 656.8 bits (1693), Expect = 1.2e-184
Identity = 341/396 (86.11%), Postives = 354/396 (89.39%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGETITAA  HED+GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES+VKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIAFADTI+LNKVDLV+S+  G GALEDLE+
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSER-GDGALEDLEF 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EI NINSLAKIIHSVRCQVDLSLILNCNAYNA NT HLE LLKESRSLST+DLHDTGVRT
Sbjct: 241 EIHNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS  +KVDLDK V SWLEEILWD+KGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISYHDKVDLDK-VRSWLEEILWDRKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
           PTR+WNNGES MNKIVFIGRNL+EDVLSETFRACAV
Sbjct: 361 PTRQWNNGESQMNKIVFIGRNLSEDVLSETFRACAV 366

BLAST of MS001003 vs. ExPASy Swiss-Prot
Match: Q9BRT8 (COBW domain-containing protein 1 OS=Homo sapiens OX=9606 GN=CBWD1 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.7e-62
Identity = 147/397 (37.03%), Postives = 224/397 (56.42%), Query Frame = 0

Query: 4   EEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAVIL 63
           EED P  V I  T +        G  + VT+ITGYLGAGK+TL+NYIL  QH KR+AVIL
Sbjct: 16  EEDCPELVPIETTQSEEEEKSGLGAKIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVIL 75

Query: 64  NEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYSIIM 123
           NEFGE   +E+++     GG L EEW+EL NGC+CC+VK S ++A+E L+Q+K ++    
Sbjct: 76  NEFGEGSALEKSLA-VSQGGELYEEWLELRNGCLCCSVKDSGLRAIENLMQKKGKF---- 135

Query: 124 LDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLDSII 183
                                   D+ILLETTGLA+P  +AS+ W+D +L S + LD II
Sbjct: 136 ------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDGII 195

Query: 184 TVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEYEIR 243
           T+VD+K     L E +      EA  Q+A AD I++NK DLV  +      ++ L   IR
Sbjct: 196 TIVDSKYGLKHLTEEKPDGLINEATRQVALADAILINKTDLVPEED-----VKKLRTTIR 255

Query: 244 NINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRTLCI 303
           +IN L +I+ + R +VDLS +L+ +A+++ +   L+  L+      T+   D  + T+  
Sbjct: 256 SINGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP--GTQPHLDQSIVTITF 315

Query: 304 SDDNKVDLDKVVHSWLEEILWDK------KGGMDVYRCKGVLSIKNSDQLHTLQAVRELY 363
                   ++ ++ +++ +LW+K         M+V R KG++SIK+  Q   +Q V ELY
Sbjct: 316 EVPGNAK-EEHLNMFIQNLLWEKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELY 375

Query: 364 EVVPTR-KWNNGESHMNKIVFIGRNLNEDVLSETFRA 394
           ++  T   W +     N++V +GRNL++D+L + F A
Sbjct: 376 DLEETPVSWKDDTERTNRLVLLGRNLDKDILKQLFIA 375

BLAST of MS001003 vs. ExPASy Swiss-Prot
Match: Q8VEH6 (COBW domain-containing protein 1 OS=Mus musculus OX=10090 GN=Cbwd1 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.0e-61
Identity = 153/397 (38.54%), Postives = 227/397 (57.18%), Query Frame = 0

Query: 2   EDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAV 61
           E  ED P  V I ET      + D    + VT++TGYLGAGK+TL+NYIL  QH ++IAV
Sbjct: 13  EYAEDCPELVPI-ETKNQEEENLDFITKIPVTIVTGYLGAGKTTLLNYILTEQHNRKIAV 72

Query: 62  ILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYSI 121
           ILNEFGE   VE+++     GG L EEW+EL NGC+CC+VK S ++A+E L+Q+K ++  
Sbjct: 73  ILNEFGEGSAVEKSLA-VSQGGELYEEWLELRNGCLCCSVKDSGLRAIENLMQKKGKF-- 132

Query: 122 IMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLDS 181
                                     D+ILLETTGLA+P  +AS+ W+D +L S + LD 
Sbjct: 133 --------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDG 192

Query: 182 IITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEYE 241
           IITVVD+K     L E +      EA  Q+A AD I++NK DLVS +      L +L   
Sbjct: 193 IITVVDSKYGLKHLTEEKPDGLVNEATRQVALADMILINKTDLVSEEE-----LNNLRTT 252

Query: 242 IRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRTL 301
           IR+IN L K++ + R +V LS IL+ +AY+  +   L+   K+ + +ST    D  + T+
Sbjct: 253 IRSINGLGKVLETQRSRVHLSNILDLHAYDILSGISLQ---KKLQHVSTAPHLDQSIVTV 312

Query: 302 CISDDNKVDLDKVVHSWLEEILWDK----KGG--MDVYRCKGVLSIKNSDQLHTLQAVRE 361
                     ++ ++ +++ +LW+K    K G  M+V R KG++SIK+  Q   +Q + E
Sbjct: 313 TFEVPGSAK-EECLNVFIQNLLWEKNVKNKDGHCMEVIRLKGLVSIKDKPQQMIVQGIHE 370

Query: 362 LYEVVPTR-KWNNGESHMNKIVFIGRNLNEDVLSETF 392
           LY++  +   W +      ++VFIGRNL++DVL + F
Sbjct: 373 LYDLEESLVNWKDDAERACQLVFIGRNLDKDVLQQLF 370

BLAST of MS001003 vs. ExPASy Swiss-Prot
Match: Q99MB4 (COBW domain-containing protein 1 OS=Rattus norvegicus OX=10116 GN=Cbwd1 PE=2 SV=2)

HSP 1 Score: 238.8 bits (608), Expect = 1.0e-61
Identity = 149/397 (37.53%), Postives = 226/397 (56.93%), Query Frame = 0

Query: 2   EDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAV 61
           E  ED P  V I         + D  + + VT++TGYLGAGK+TL+NYIL  QH ++IAV
Sbjct: 13  EYAEDCPELVPIETKHQEKEENLDFIIKIPVTIVTGYLGAGKTTLLNYILTEQHNRKIAV 72

Query: 62  ILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYSI 121
           ILNEFGE   VE+++     GG L EEW+EL NGC+CC+VK + ++A+E L+Q+K ++  
Sbjct: 73  ILNEFGEGSAVEKSLA-VSQGGELYEEWLELRNGCLCCSVKDNGLKAIENLMQKKGKF-- 132

Query: 122 IMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLDS 181
                                     D+ILLETTGLA+P  +AS+ W+D +L S + LD 
Sbjct: 133 --------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDG 192

Query: 182 IITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEYE 241
           IITVVD+K     L E +      EA  Q+A AD I++NK DLVS +      L  L   
Sbjct: 193 IITVVDSKYGLKHLTEEKPDGLVNEATRQVALADMILINKTDLVSEEE-----LNKLRTT 252

Query: 242 IRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRTL 301
           IR+IN L K++ + R +  LS IL+ +AY+  +   L+   K+ + +ST    D  + T+
Sbjct: 253 IRSINGLGKVLETQRSRTHLSNILDLHAYDTLSGISLQ---KKLQHVSTAPHLDQSIVTV 312

Query: 302 CISDDNKVDLDKVVHSWLEEILWDK----KGG--MDVYRCKGVLSIKNSDQLHTLQAVRE 361
                   + ++ ++ +++ +LW+K    K G  M+V R KG++SIK+  Q   +Q + E
Sbjct: 313 TFDVPGSAE-EESLNVFIQNLLWEKNVKNKDGRCMEVIRLKGLVSIKDKPQQMIVQGIHE 371

Query: 362 LYEVVPTR-KWNNGESHMNKIVFIGRNLNEDVLSETF 392
           LYE+  +R  W +      ++VFIG+NL++D+L + F
Sbjct: 373 LYELEESRVNWKDDAERACQLVFIGKNLDKDILQQLF 371

BLAST of MS001003 vs. ExPASy Swiss-Prot
Match: Q8IUF1 (COBW domain-containing protein 2 OS=Homo sapiens OX=9606 GN=CBWD2 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.0e-61
Identity = 145/397 (36.52%), Postives = 224/397 (56.42%), Query Frame = 0

Query: 4   EEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAVIL 63
           EED P  V +  T +        G  + VT+ITGYLGAGK+TL+NYIL  QH KR+AVIL
Sbjct: 16  EEDCPELVPMETTQSEEEEKSGLGAKIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVIL 75

Query: 64  NEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYSIIM 123
           NEFGE   +E+++     GG L EEW+EL NGC+CC+VK + ++A+E L+Q+K ++    
Sbjct: 76  NEFGEGSALEKSLA-VSQGGELYEEWLELRNGCLCCSVKDNGLRAIENLMQKKGKF---- 135

Query: 124 LDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLDSII 183
                                   D+ILLETTGLA+P  +AS+ W+D +L S + LD II
Sbjct: 136 ------------------------DYILLETTGLADPGAVASMFWVDAELGSDIYLDGII 195

Query: 184 TVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEYEIR 243
           T+VD+K     L E +      EA  Q+A AD I++NK DLV  +      ++ L   IR
Sbjct: 196 TIVDSKYGLKHLAEEKPDGLINEATRQVALADAILINKTDLVPEED-----VKKLRATIR 255

Query: 244 NINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRTLCI 303
           +IN L +I+ + R +VDLS +L+ +A+++ +   L+  L+      T+   D  + T+  
Sbjct: 256 SINGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP--GTQPHLDQSIVTITF 315

Query: 304 SDDNKVDLDKVVHSWLEEILWDK------KGGMDVYRCKGVLSIKNSDQLHTLQAVRELY 363
                   ++ ++ +++ +LW+K         M+V R KG++SIK+  Q   +Q V ELY
Sbjct: 316 EVPGNAK-EEHLNMFIQNLLWEKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELY 375

Query: 364 EVVPTR-KWNNGESHMNKIVFIGRNLNEDVLSETFRA 394
           ++  T   W +     N++V +GRNL++D+L + F A
Sbjct: 376 DLEETPVSWKDDTERTNRLVLLGRNLDKDILKQLFIA 375

BLAST of MS001003 vs. ExPASy Swiss-Prot
Match: Q5JTY5 (COBW domain-containing protein 3 OS=Homo sapiens OX=9606 GN=CBWD3 PE=1 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.5e-60
Identity = 146/397 (36.78%), Postives = 222/397 (55.92%), Query Frame = 0

Query: 4   EEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIAVIL 63
           EED P  V I  T +        G  + VT+ITGYLGAGK+TL+NYIL  QH KR+AVIL
Sbjct: 16  EEDCPELVPIETTQSEEEEKSGLGAKIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVIL 75

Query: 64  NEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYSIIM 123
           NE GE   +E+++     GG L EEW+EL NGC+CC+VK + ++A+E L+Q+K ++    
Sbjct: 76  NESGEGSALEKSLA-VSQGGELYEEWLELRNGCLCCSVKDNGLRAIENLMQKKGKF---- 135

Query: 124 LDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLDSII 183
                                   D ILLETTGLA+P  +AS+ W+D +L S + LD II
Sbjct: 136 ------------------------DDILLETTGLADPGAVASMFWVDAELGSDIYLDGII 195

Query: 184 TVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEYEIR 243
           T+VD+K     L E +      EA  Q+A AD I++NK DLV  +      ++ L   IR
Sbjct: 196 TIVDSKYGLKHLTEEKPDGLINEATRQVALADIILINKTDLVPEED-----VKKLRTTIR 255

Query: 244 NINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRTLCI 303
           +IN L +I+ + R +VDLS +L+ +A+++ +   L+  L+      T+   D  + T+  
Sbjct: 256 SINGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP--GTQPHLDQSIVTITF 315

Query: 304 SDDNKVDLDKVVHSWLEEILWDK------KGGMDVYRCKGVLSIKNSDQLHTLQAVRELY 363
                   ++ ++ +++ +LW+K         M+V R KG++SIK+  Q   +Q V ELY
Sbjct: 316 EVPGNAK-EEHLNMFIQNLLWEKNVRNKDNHCMEVIRLKGLVSIKDKSQQVIVQGVHELY 375

Query: 364 EVVPTR-KWNNGESHMNKIVFIGRNLNEDVLSETFRA 394
           ++  T   W +     N++V IGRNL++D+L + F A
Sbjct: 376 DLEETPVSWKDDTERTNRLVLIGRNLDKDILKQLFIA 375

BLAST of MS001003 vs. ExPASy TrEMBL
Match: A0A6J1BTI0 (COBW domain-containing protein 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005195 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 9.4e-199
Identity = 362/396 (91.41%), Postives = 364/396 (91.92%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLESTVKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNL+FQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY
Sbjct: 181 SIITVVDAKNLNFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCISDDNKVDLDK VHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISDDNKVDLDK-VHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
           PTRKWNNGES MNKIVFIGRNLNEDVL ET RACAV
Sbjct: 361 PTRKWNNGESPMNKIVFIGRNLNEDVLRETXRACAV 367

BLAST of MS001003 vs. ExPASy TrEMBL
Match: A0A5A7VCN1 (COBW domain-containing protein 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00610 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.4e-186
Identity = 339/395 (85.82%), Postives = 353/395 (89.37%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGET+TA   HED GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETVTATQQHEDGGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES++KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAFHQI FADTI+LNKVDLVSS   G GALEDLEY
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIVFADTIILNKVDLVSSDR-GDGALEDLEY 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLIL+CN+YNA NT HLEALLKESRSLST+DLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILDCNSYNAANTAHLEALLKESRSLSTQDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS+ +KVDLDKVVHSWLEEILW+KKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISEHHKVDLDKVVHSWLEEILWEKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACA 396
           PTR+W+NGES MNKIVFIGRNLNEDVLS+TFR CA
Sbjct: 361 PTRQWSNGESQMNKIVFIGRNLNEDVLSKTFRECA 366

BLAST of MS001003 vs. ExPASy TrEMBL
Match: A0A6J1HE98 (COBW domain-containing protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111462688 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.6e-185
Identity = 342/396 (86.36%), Postives = 355/396 (89.65%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGETITAA  HED+GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES+VKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAF+QIAFADTI+LNKVDLV+S+  G GALEDLE+
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSER-GDGALEDLEF 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLILNCNAYNA NT HLE LLKESRSLST+DLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS  +KVDLDK V SWLEEILWD+KGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISYHDKVDLDK-VRSWLEEILWDRKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
           PTR+WNNGES MNKIVFIGRNL+EDVLSETFRACAV
Sbjct: 361 PTRQWNNGESQMNKIVFIGRNLSEDVLSETFRACAV 366

BLAST of MS001003 vs. ExPASy TrEMBL
Match: A0A1S4DVA6 (COBW domain-containing protein 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488574 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.0e-184
Identity = 338/395 (85.57%), Postives = 352/395 (89.11%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGET+TA   HED GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETVTATQQHEDGGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES++KLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSIKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDAKNLHFQLNEHRSSSSFPEAFHQI FADTI+LNKVDLVSS   G GALEDLEY
Sbjct: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIVFADTIILNKVDLVSSDR-GDGALEDLEY 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHSVRCQVDLSLIL+CN+YNA NT HLEALLKESRSLST+DLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSVRCQVDLSLILDCNSYNAANTAHLEALLKESRSLSTQDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS+ +KVDLDK VHSWLEEILW+KKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISEHHKVDLDK-VHSWLEEILWEKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACA 396
           PTR+W+NGES MNKIVFIGRNLNEDVLS+TFR CA
Sbjct: 361 PTRQWSNGESQMNKIVFIGRNLNEDVLSKTFRECA 365

BLAST of MS001003 vs. ExPASy TrEMBL
Match: A0A6J1K8W5 (COBW domain-containing protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111492164 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.5e-184
Identity = 339/396 (85.61%), Postives = 354/396 (89.39%), Query Frame = 0

Query: 1   MEDEEDPPLAVEIGETITAAGPHEDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60
           MEDEEDPPLAVEIGETITAA  HED+GVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA
Sbjct: 1   MEDEEDPPLAVEIGETITAAERHEDDGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 60

Query: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 120
           VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE   
Sbjct: 61  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKE--- 120

Query: 121 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 180
                                    RLDHILLETTGLANPAPLASVLWLDDQLES+VKLD
Sbjct: 121 -------------------------RLDHILLETTGLANPAPLASVLWLDDQLESSVKLD 180

Query: 181 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 240
           SIITVVDA+NLHFQLNEHRSSSSFPEAF+QIAFADTI+LNKVDLV+S+  G GALEDLE+
Sbjct: 181 SIITVVDARNLHFQLNEHRSSSSFPEAFYQIAFADTIILNKVDLVTSER-GDGALEDLEF 240

Query: 241 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 300
           EIRNINSLAKIIHS+RCQVDLSLILNCNAYNA NT HLE LLKESRSLST+DLHDTGVRT
Sbjct: 241 EIRNINSLAKIIHSIRCQVDLSLILNCNAYNAANTAHLEELLKESRSLSTKDLHDTGVRT 300

Query: 301 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 360
           LCIS  +KVDLDK V SWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYE+V
Sbjct: 301 LCISYHDKVDLDK-VRSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEIV 360

Query: 361 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
           PTR+WNN ES MNKIVFIGRNL+E+VLSETFRACAV
Sbjct: 361 PTRQWNNRESQMNKIVFIGRNLSENVLSETFRACAV 366

BLAST of MS001003 vs. TAIR 10
Match: AT1G26520.1 (Cobalamin biosynthesis CobW-like protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.5e-143
Identity = 261/394 (66.24%), Postives = 307/394 (77.92%), Query Frame = 0

Query: 2   EDEEDPPLAVEIGETITAAGPH-EDEGVSVGVTVITGYLGAGKSTLVNYILNSQHGKRIA 61
           +D+E+PP+AV+I   ++    H   + VSVGV+VITGYLGAGKSTLVNYILN +HGKRIA
Sbjct: 13  DDDEEPPMAVQIHPDVSVGKVHSSSDTVSVGVSVITGYLGAGKSTLVNYILNGKHGKRIA 72

Query: 62  VILNEFGEEIGVERAMINEGDGGALVEEWVELANGCICCTVKHSLVQALEQLVQRKERYS 121
           VILNEFGEEIGVERAMINEG+ GA+VEEWVELANGC+CCTVKHSLVQALEQLVQRK+   
Sbjct: 73  VILNEFGEEIGVERAMINEGEEGAIVEEWVELANGCVCCTVKHSLVQALEQLVQRKD--- 132

Query: 122 IIMLDFSADFVKNTLIIEEFFALVSRLDHILLETTGLANPAPLASVLWLDDQLESTVKLD 181
                                    RLDHILLETTGLANPAPLAS+LWLDDQLES VKLD
Sbjct: 133 -------------------------RLDHILLETTGLANPAPLASILWLDDQLESEVKLD 192

Query: 182 SIITVVDAKNLHFQLNEHRSSSSFPEAFHQIAFADTIVLNKVDLVSSKHGGGGALEDLEY 241
            I+TVVDAKNL FQLNE R SSSFPEAF+QIAFADTI++NKVDL+S +       ++LE 
Sbjct: 193 CIVTVVDAKNLRFQLNERRDSSSFPEAFNQIAFADTIIMNKVDLISQEES-----DELEK 252

Query: 242 EIRNINSLAKIIHSVRCQVDLSLILNCNAYNATNTGHLEALLKESRSLSTRDLHDTGVRT 301
           EI +INSLA +I SVRCQVDLS ILNC AY++T+   LE+LL+ ++SL+T DLHD+GVRT
Sbjct: 253 EIHSINSLANVIRSVRCQVDLSNILNCQAYDSTHVSRLESLLEANKSLTTTDLHDSGVRT 312

Query: 302 LCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVV 361
           LCIS+   ++LDK V  WLEEILWDKK  MDVYRCK VLSI+NSDQ+H LQAVR++YE+V
Sbjct: 313 LCISEPQPINLDK-VRLWLEEILWDKKSEMDVYRCKAVLSIQNSDQMHILQAVRDIYEIV 372

Query: 362 PTRKWNNGESHMNKIVFIGRNLNEDVLSETFRAC 395
           P RKW+  E+  NKIVFIG  L+E+VL    R C
Sbjct: 373 PARKWSEEENRTNKIVFIGHKLDEEVLRSGLRDC 372

BLAST of MS001003 vs. TAIR 10
Match: AT1G15730.1 (Cobalamin biosynthesis CobW-like protein )

HSP 1 Score: 226.1 bits (575), Expect = 5.0e-59
Identity = 138/395 (34.94%), Postives = 209/395 (52.91%), Query Frame = 0

Query: 30  VGVTVITGYLGAGKSTLVNYILNSQHGKRIAVILNEFGEEIGVERAMINEGDGGALVEEW 89
           +  T+ITG+LG+GK+TL+N+IL   HGKRIAVI NEFG E+ ++ +++     GA  E+ 
Sbjct: 92  IPATIITGFLGSGKTTLLNHILTGDHGKRIAVIENEFG-EVDIDGSLVAAQTAGA--EDI 151

Query: 90  VELANGCICCTVKHSLVQALEQLVQRKERYSIIMLDFSADFVKNTLIIEEFFALVSRLDH 149
           + L NGC+CCTV+  LV+ + ++VQ K+                            R DH
Sbjct: 152 MMLNNGCLCCTVRGDLVRMISEMVQTKK---------------------------GRFDH 211

Query: 150 ILLETTGLANPAPLASVLWLDDQLESTVKLDSIITVVDAKNLHFQLNEHRSSSSFPEAFH 209
           I++ETTGLANPAP+    + +D++ + VKLD ++T+VDAK+    L+E +      EA  
Sbjct: 212 IVIETTGLANPAPIIQTFYAEDEIFNDVKLDGVVTLVDAKHARLHLDEVKPEGYVNEAVE 271

Query: 210 QIAFADTIVLNKVDLVSSKHGGGGALEDLEYEIRNINSLAKIIHSVRCQVDLSLILNCNA 269
           QIA+AD I++NK DLV     G   L  +   I+ INS+A +  +   +VDL  +L    
Sbjct: 272 QIAYADRIIVNKTDLV-----GEPELASVMQRIKTINSMAHMKRTKYGKVDLDYVLGIGG 331

Query: 270 YNAT--------------------NTGH--------LEALLKESRSLSTRDLHDTGVRTL 329
           ++                      + GH         E   +     S    HD GV ++
Sbjct: 332 FDLERIESSVNEEEKEDREGHDDHHHGHDCHDHHNEHEHEHEHEHHHSHDHTHDPGVGSV 391

Query: 330 CISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEVVP 389
            I  +  +DL+K  + WL  +L+ +    D+YR KG+LS+++ D+    Q V E++E  P
Sbjct: 392 SIVCEGDLDLEK-ANMWLGALLYQR--SEDIYRMKGILSVQDMDERFVFQGVHEIFEGSP 448

Query: 390 TRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
            R W   E+  NKIVFIG+NLN + L   FRAC +
Sbjct: 452 DRLWRKDETRTNKIVFIGKNLNREELEMGFRACLI 448

BLAST of MS001003 vs. TAIR 10
Match: AT1G80480.1 (plastid transcriptionally active 17 )

HSP 1 Score: 218.4 bits (555), Expect = 1.0e-56
Identity = 132/397 (33.25%), Postives = 207/397 (52.14%), Query Frame = 0

Query: 30  VGVTVITGYLGAGKSTLVNYILNSQHGKRIAVILNEFGEEIGVERAMINEGDGGALVEEW 89
           +  T+ITG+LG+GK+TL+N+IL   HGKRIAVI NEFG E+ ++ +++     GA  E+ 
Sbjct: 89  IPATIITGFLGSGKTTLLNHILTRDHGKRIAVIENEFG-EVDIDGSLVASKSIGA--EDI 148

Query: 90  VELANGCICCTVKHSLVQALEQLVQRKERYSIIMLDFSADFVKNTLIIEEFFALVSRLDH 149
           V L NGC+CCTV+  LV+ + +LV  K+                            + DH
Sbjct: 149 VMLNNGCLCCTVRGDLVRMIGELVNTKK---------------------------GKFDH 208

Query: 150 ILLETTGLANPAPLASVLWLDDQLESTVKLDSIITVVDAKNLHFQLNEHRSSSSFPEAFH 209
           I++ETTGLANPAP+    + ++++ + VKLD ++T+VDAK+    L+E +      EA  
Sbjct: 209 IVIETTGLANPAPIIQTFYAEEEIFNDVKLDGVVTLVDAKHARLHLDEVKPEGVVNEAVE 268

Query: 210 QIAFADTIVLNKVDLVSSKHGGGGALEDLEYEIRNINSLAKIIHSVRCQVDLSLILNCNA 269
           QIA+AD I++NK DLV     G   L  +   I+ INS+A++  +    VDL  +L    
Sbjct: 269 QIAYADRIIVNKTDLV-----GEAELGSVVQRIKTINSMAQMTRTKYGNVDLDYVLGIGG 328

Query: 270 YNATNTGHLEALLKESRSLSTRD------------------------------LHDTGVR 329
           ++      +E+ + E       D                               HD GV 
Sbjct: 329 FDLE---RIESSVNEDDKGDHHDHDHDHHHDHNHDHDHHHHDGHDHHHHSHDHTHDPGVS 388

Query: 330 TLCISDDNKVDLDKVVHSWLEEILWDKKGGMDVYRCKGVLSIKNSDQLHTLQAVRELYEV 389
           ++ I  +  +DL+K  + WL  +L ++    D+YR KG+LS+   ++    Q V ++++ 
Sbjct: 389 SVSIVCEGSLDLEK-ANMWLGTLLMER--SEDIYRMKGLLSVHTMEERFVFQGVHDIFQG 444

Query: 390 VPTRKWNNGESHMNKIVFIGRNLNEDVLSETFRACAV 397
            P R W   E  +NKIVFIG+NLN + L + F+AC +
Sbjct: 449 SPDRLWGREEERVNKIVFIGKNLNREELEKGFKACLI 444