MS000977 (gene) Bitter gourd (TR) v1

Overview
NameMS000977
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsmall ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial-like
Locationscaffold36: 596600 .. 599184 (+)
RNA-Seq ExpressionMS000977
SyntenyMS000977
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATCGCTTCCATTTCTATGCTCCGCCACCACACCGCCGCCGCCACTGCGTCAATCCCCGCCTTCGGCAATGTACTCCGGCGATTCTTTAACGTCCGATGCTTTTCAGTTGTTGCCAAACAGCATAACCCTAACAATGTCTCCAATAAGCCTCCGCCCAGCAAGAATCTCCTCCGAGCAAAGCACACGTTCAAGGATTATTCATCCCTTGCACCGGCTCTCTCTCCTCAAGACAATCCTCCTCTCTCCGAATCCCAAGCCATCGGCACTGTCGCTGCTGCCCAAGCCAATTTCATGCGCGTAATTGTTCAGTCGCTTCCCTCTCAACTTTCTGACAGTTCGAACCAGGATTCGGAGCCTTCTGGAAGCTTAGAAGGCGATTCTTCGGAATCCTCTAGGCTTGGAGTTGAGCTGCTATGCGTGGTGAAGGCGGTGCTCAAGAAGATAAAGAGGAGAGTTCTAGTCGGAGACAAGGTGGTTGTTGGTTCCATTGATTGGGTGGACCGCCGGGGAATGATCGAGAATGTCTTCCAAAGGAGCTCGGAGATTCTTGATCCTCCGGTGGCCAATGTGGACCATTTGCTTGTGCTCTTCTCCATGGATCAGCCGAAGTTAGAGCCGTTTACACTCACGAGGTTTCTGATTGAGGCGGAATCTACCGGAATTCCACTGACGCTGGGTCTCAATAAGTTAGAACTCGTCGCTGAAGAGGTTGTAGCTTCAATCTTTTACTTACAGCTTTAATCACTTAGCTTTCTGTTAACTAGTTTCAACGCCTACGCTGCATCTGAAGTCTCTTGCATCATCTTCGAATACTGGTCCTCTAGAATAATAGTGATGTAGTGTAGCAGAAAGATATTCATAAATGTATTTAGTTTTGCTATTTTACTCTTACTTTGTCATGAATGCTTCTTTTTTCTCATAGTAAGATGCGTTTATCTCGTAGCATGAAATTTTGTTCTTGCGTTACTTGTCACATAGCGTATTCTTTACTCTGGGAACCTGCTTGAATCTCTAGAAGAGCAGTCATTGACCAAACTTTCTCACAATTATGCATGCATTTCCTTTCTTGATGCTGATAGTTGTTGGTAATGGCATCTGCAGACACTAGTTTCCTGGAAGTCAAGACTGCGAAGCTGGGGCTATGAACCACTTTTCTGCAGTGTCCAAACCAAGACAGGACTCGATTCCCTTGCATATATTTTGAGAGATCAAACTACAGTTATCGTGGGTCCAAGTGGAGTTGGGAAATCAAGTCTCATCAATGCTCTGAGAAACAATGACCGTGGCGTCGATGCTGTAGAGGGAGATAATTGGTTTGATCCTGTGTGTTTGATCATTCTTTGAATTTCTATTATGTTTGTTTTGTTGGTCATCTATTCACTCTGGGACTTTCTATGATCACTAGGCTCATAAAAGTTTCTATATTTTTCTGGTCTATTAGCAGATTCTAGGTAGCAAGTGGTTCGAGGATCAGCGTGTTGCAGAAGTCTCAACAAGAAGTGGTAGAGGGAAGCATACGACTCGTCATGTTTCTCTCCTGCCGCTGTCGGGAGGGGGCTATCTGGCAGATACTCCTGGTTTTAACCAACCCAGTTTGATGAAAATAACCAAGCAGTCTCTTGCCCAAGCCTTCCCTGAGGTTCATGCTAATTTAGGACATGATAATGATTGGAATCTTTTTTGAAAAATTTCTGTGGCATTTCTTGGGTTCAACTAAATTTTCTATGCAGATCCAGAAAATGCTTCTTGCCAACGAACCTGCTAGATGCTCTTTTAATGATTGTTTACATCTTGGAGAACCGGGCTGTGTTATTAAAGGAGATTGGGAGAGATACCAATACTATTTCCAACTTCTTGATGAAATCAGAATCAGGGAAGAATTTCAGTTGAGAACATTTGGAACCAAAAGAGAGGGGGATGTGAGGTATGTTCACCTTAGCTCTGTGATGCTATTTCCTTTCAATATTCTCCTTTATATTTTAAACAAGTTCCAATACAAAACATTGCCTTTAAATGTTTTTGTTTAGTGGTAGTGCCTTTGTTCTATTGGCAATACTGCTGAGACTTTTTCAGTAATAAGGAAATAGGAATCCTCCTTCACTCTGTAATTATATATCAATTTGGTATCCTAATAACATTCGAGGAACTTAGACTATGCAACAATGAGATTGATAATCCCATTTGATACAATATTCTGATTGCAGTTTATATCCATTTATATTTATGTAGCACATCTGAGACTTCAGAATTTGGTGCATCACTGTCGAAGTAACGAGTAACACTAGAACTTGTTTATCATGAATAACGATATTGTTCAGTTAGCTCTGCATCCTTAATCCTTAATCCTTATACGAGTCCAAAATCAAAATGATGGAACTTTTCTTTTCAGAATTAAGGTTGGAGATATGGGTGCTCAGCAAGCAGAGCCAAGGTTGGAGCTGAAGAAGCACAGGAGACAATCTCGTAAGCGAGTGAACCAGTCAATACTTAACGAGTTGGATGAGTTGGAAGATGAAGACGAAGACGATTCATTGGACAAGGAGGACCCCATTTTAAAGGCAATGAGGAATGAACAGCAA

mRNA sequence

ATGGCGATCGCTTCCATTTCTATGCTCCGCCACCACACCGCCGCCGCCACTGCGTCAATCCCCGCCTTCGGCAATGTACTCCGGCGATTCTTTAACGTCCGATGCTTTTCAGTTGTTGCCAAACAGCATAACCCTAACAATGTCTCCAATAAGCCTCCGCCCAGCAAGAATCTCCTCCGAGCAAAGCACACGTTCAAGGATTATTCATCCCTTGCACCGGCTCTCTCTCCTCAAGACAATCCTCCTCTCTCCGAATCCCAAGCCATCGGCACTGTCGCTGCTGCCCAAGCCAATTTCATGCGCGTAATTGTTCAGTCGCTTCCCTCTCAACTTTCTGACAGTTCGAACCAGGATTCGGAGCCTTCTGGAAGCTTAGAAGGCGATTCTTCGGAATCCTCTAGGCTTGGAGTTGAGCTGCTATGCGTGGTGAAGGCGGTGCTCAAGAAGATAAAGAGGAGAGTTCTAGTCGGAGACAAGGTGGTTGTTGGTTCCATTGATTGGGTGGACCGCCGGGGAATGATCGAGAATGTCTTCCAAAGGAGCTCGGAGATTCTTGATCCTCCGGTGGCCAATGTGGACCATTTGCTTGTGCTCTTCTCCATGGATCAGCCGAAGTTAGAGCCGTTTACACTCACGAGGTTTCTGATTGAGGCGGAATCTACCGGAATTCCACTGACGCTGGGTCTCAATAAGTTAGAACTCGTCGCTGAAGAGACACTAGTTTCCTGGAAGTCAAGACTGCGAAGCTGGGGCTATGAACCACTTTTCTGCAGTGTCCAAACCAAGACAGGACTCGATTCCCTTGCATATATTTTGAGAGATCAAACTACAGTTATCGTGGGTCCAAGTGGAGTTGGGAAATCAAGTCTCATCAATGCTCTGAGAAACAATGACCGTGGCGTCGATGCTGTAGAGGGAGATAATTGGTTTGATCCTATTCTAGGTAGCAAGTGGTTCGAGGATCAGCGTGTTGCAGAAGTCTCAACAAGAAGTGGTAGAGGGAAGCATACGACTCGTCATGTTTCTCTCCTGCCGCTGTCGGGAGGGGGCTATCTGGCAGATACTCCTGGTTTTAACCAACCCAGTTTGATGAAAATAACCAAGCAGTCTCTTGCCCAAGCCTTCCCTGAGATCCAGAAAATGCTTCTTGCCAACGAACCTGCTAGATGCTCTTTTAATGATTGTTTACATCTTGGAGAACCGGGCTGTGTTATTAAAGGAGATTGGGAGAGATACCAATACTATTTCCAACTTCTTGATGAAATCAGAATCAGGGAAGAATTTCAGTTGAGAACATTTGGAACCAAAAGAGAGGGGGATGTGAGAATTAAGGTTGGAGATATGGGTGCTCAGCAAGCAGAGCCAAGGTTGGAGCTGAAGAAGCACAGGAGACAATCTCGTAAGCGAGTGAACCAGTCAATACTTAACGAGTTGGATGAGTTGGAAGATGAAGACGAAGACGATTCATTGGACAAGGAGGACCCCATTTTAAAGGCAATGAGGAATGAACAGCAA

Coding sequence (CDS)

ATGGCGATCGCTTCCATTTCTATGCTCCGCCACCACACCGCCGCCGCCACTGCGTCAATCCCCGCCTTCGGCAATGTACTCCGGCGATTCTTTAACGTCCGATGCTTTTCAGTTGTTGCCAAACAGCATAACCCTAACAATGTCTCCAATAAGCCTCCGCCCAGCAAGAATCTCCTCCGAGCAAAGCACACGTTCAAGGATTATTCATCCCTTGCACCGGCTCTCTCTCCTCAAGACAATCCTCCTCTCTCCGAATCCCAAGCCATCGGCACTGTCGCTGCTGCCCAAGCCAATTTCATGCGCGTAATTGTTCAGTCGCTTCCCTCTCAACTTTCTGACAGTTCGAACCAGGATTCGGAGCCTTCTGGAAGCTTAGAAGGCGATTCTTCGGAATCCTCTAGGCTTGGAGTTGAGCTGCTATGCGTGGTGAAGGCGGTGCTCAAGAAGATAAAGAGGAGAGTTCTAGTCGGAGACAAGGTGGTTGTTGGTTCCATTGATTGGGTGGACCGCCGGGGAATGATCGAGAATGTCTTCCAAAGGAGCTCGGAGATTCTTGATCCTCCGGTGGCCAATGTGGACCATTTGCTTGTGCTCTTCTCCATGGATCAGCCGAAGTTAGAGCCGTTTACACTCACGAGGTTTCTGATTGAGGCGGAATCTACCGGAATTCCACTGACGCTGGGTCTCAATAAGTTAGAACTCGTCGCTGAAGAGACACTAGTTTCCTGGAAGTCAAGACTGCGAAGCTGGGGCTATGAACCACTTTTCTGCAGTGTCCAAACCAAGACAGGACTCGATTCCCTTGCATATATTTTGAGAGATCAAACTACAGTTATCGTGGGTCCAAGTGGAGTTGGGAAATCAAGTCTCATCAATGCTCTGAGAAACAATGACCGTGGCGTCGATGCTGTAGAGGGAGATAATTGGTTTGATCCTATTCTAGGTAGCAAGTGGTTCGAGGATCAGCGTGTTGCAGAAGTCTCAACAAGAAGTGGTAGAGGGAAGCATACGACTCGTCATGTTTCTCTCCTGCCGCTGTCGGGAGGGGGCTATCTGGCAGATACTCCTGGTTTTAACCAACCCAGTTTGATGAAAATAACCAAGCAGTCTCTTGCCCAAGCCTTCCCTGAGATCCAGAAAATGCTTCTTGCCAACGAACCTGCTAGATGCTCTTTTAATGATTGTTTACATCTTGGAGAACCGGGCTGTGTTATTAAAGGAGATTGGGAGAGATACCAATACTATTTCCAACTTCTTGATGAAATCAGAATCAGGGAAGAATTTCAGTTGAGAACATTTGGAACCAAAAGAGAGGGGGATGTGAGAATTAAGGTTGGAGATATGGGTGCTCAGCAAGCAGAGCCAAGGTTGGAGCTGAAGAAGCACAGGAGACAATCTCGTAAGCGAGTGAACCAGTCAATACTTAACGAGTTGGATGAGTTGGAAGATGAAGACGAAGACGATTCATTGGACAAGGAGGACCCCATTTTAAAGGCAATGAGGAATGAACAGCAA

Protein sequence

MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEETLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDELEDEDEDDSLDKEDPILKAMRNEQQ
Homology
BLAST of MS000977 vs. NCBI nr
Match: XP_022132325.1 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 979.2 bits (2530), Expect = 1.4e-281
Identity = 503/505 (99.60%), Postives = 503/505 (99.60%), Query Frame = 0

Query: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60
           MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR
Sbjct: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60

Query: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120
           AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE
Sbjct: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120

Query: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180
           PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR
Sbjct: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180

Query: 181 SSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEETL 240
           SSEILDPPVANVDHLLVLFSMDQPKLE FTLTRFLIEAESTGIPLTLGLNKLELV EETL
Sbjct: 181 SSEILDPPVANVDHLLVLFSMDQPKLESFTLTRFLIEAESTGIPLTLGLNKLELVDEETL 240

Query: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300
           VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG
Sbjct: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300

Query: 301 VDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQ 360
           VDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQ
Sbjct: 301 VDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQ 360

Query: 361 PSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLD 420
           PSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLD
Sbjct: 361 PSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLD 420

Query: 421 EIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDE 480
           EIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDE
Sbjct: 421 EIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDE 480

Query: 481 LEDEDEDDSLDKEDPILKAMRNEQQ 506
           LEDEDEDDSLDKEDPILKAMRNEQQ
Sbjct: 481 LEDEDEDDSLDKEDPILKAMRNEQQ 505

BLAST of MS000977 vs. NCBI nr
Match: XP_022132324.1 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 974.5 bits (2518), Expect = 3.4e-280
Identity = 503/506 (99.41%), Postives = 503/506 (99.41%), Query Frame = 0

Query: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60
           MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR
Sbjct: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60

Query: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120
           AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE
Sbjct: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120

Query: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180
           PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR
Sbjct: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180

Query: 181 SSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEETL 240
           SSEILDPPVANVDHLLVLFSMDQPKLE FTLTRFLIEAESTGIPLTLGLNKLELV EETL
Sbjct: 181 SSEILDPPVANVDHLLVLFSMDQPKLESFTLTRFLIEAESTGIPLTLGLNKLELVDEETL 240

Query: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300
           VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG
Sbjct: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300

Query: 301 VDAVEGDNWFDP-ILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFN 360
           VDAVEGDNWFDP ILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFN
Sbjct: 301 VDAVEGDNWFDPQILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFN 360

Query: 361 QPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLL 420
           QPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLL
Sbjct: 361 QPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLL 420

Query: 421 DEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELD 480
           DEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELD
Sbjct: 421 DEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELD 480

Query: 481 ELEDEDEDDSLDKEDPILKAMRNEQQ 506
           ELEDEDEDDSLDKEDPILKAMRNEQQ
Sbjct: 481 ELEDEDEDDSLDKEDPILKAMRNEQQ 506

BLAST of MS000977 vs. NCBI nr
Match: XP_023536955.1 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 901.7 bits (2329), Expect = 2.8e-258
Identity = 462/507 (91.12%), Postives = 485/507 (95.66%), Query Frame = 0

Query: 1   MAIASISMLRHHTA--AATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNL 60
           MAIASIS+LRHH+   AA AS+PAFGN+LRR FNVRC S+VAKQHNPNNVSNKPPPSKNL
Sbjct: 1   MAIASISILRHHSTSIAAIASVPAFGNILRRCFNVRCLSIVAKQHNPNNVSNKPPPSKNL 60

Query: 61  LRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQD 120
           LRAKHTFK+YSSLAP LSPQD PPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSN D
Sbjct: 61  LRAKHTFKNYSSLAPVLSPQDIPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNSD 120

Query: 121 SEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180
           SE SGSLEGDSS+SSRLG+ELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF
Sbjct: 121 SERSGSLEGDSSDSSRLGIELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180

Query: 181 QRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEE 240
           QRSSEILDPPVANVDHLLVLFS+DQPKLEPFTLTRFL+EAESTGIPLTLGLNKLELV EE
Sbjct: 181 QRSSEILDPPVANVDHLLVLFSIDQPKLEPFTLTRFLVEAESTGIPLTLGLNKLELVDEE 240

Query: 241 TLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNND 300
           T+VSWKSRLRSWGY+PLFCSVQT+ GLDSLAYILRDQTTVIVGPSGVGKSSLINALR+N+
Sbjct: 241 TVVSWKSRLRSWGYDPLFCSVQTRIGLDSLAYILRDQTTVIVGPSGVGKSSLINALRSNN 300

Query: 301 RGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF 360
           R  DAVEGDNWFDPI  SKWFEDQRVAEVSTRSGRGKHTTRHVSLLPL GGGYLADTPGF
Sbjct: 301 RAADAVEGDNWFDPIRDSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLMGGGYLADTPGF 360

Query: 361 NQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQL 420
           NQPSLMKITKQSLAQAFPEI+  LLA+EPA+CSFNDCLHLGEPGCVI GDWERYQYYFQL
Sbjct: 361 NQPSLMKITKQSLAQAFPEIRNKLLASEPAKCSFNDCLHLGEPGCVINGDWERYQYYFQL 420

Query: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480
           LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL
Sbjct: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480

Query: 481 DELEDEDEDDSLDKEDPILKAMRNEQQ 506
           DELEDED DDSL+++DPIL+AMRNE +
Sbjct: 481 DELEDED-DDSLEEDDPILRAMRNENK 506

BLAST of MS000977 vs. NCBI nr
Match: XP_022951811.1 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 898.3 bits (2320), Expect = 3.1e-257
Identity = 461/507 (90.93%), Postives = 483/507 (95.27%), Query Frame = 0

Query: 1   MAIASISMLRHHTA--AATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNL 60
           MAIASISMLRHH+   AA AS+P FGNVLRR FNVRC S+VAKQHNPNNVSNKPPPSKNL
Sbjct: 1   MAIASISMLRHHSTSIAAIASVPGFGNVLRRCFNVRCLSIVAKQHNPNNVSNKPPPSKNL 60

Query: 61  LRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQD 120
           LRAKHTFK+YSSLAP LSPQD PPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSN D
Sbjct: 61  LRAKHTFKNYSSLAPVLSPQDIPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNND 120

Query: 121 SEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180
           SE SGSLEGDS +SSRLG+ELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF
Sbjct: 121 SERSGSLEGDSLDSSRLGIELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180

Query: 181 QRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEE 240
           QRSSEILDPPVANVDHLLVLFS++QPKLEPFTLTRFL+EAESTGIPLTLGLNKLELV EE
Sbjct: 181 QRSSEILDPPVANVDHLLVLFSIEQPKLEPFTLTRFLVEAESTGIPLTLGLNKLELVDEE 240

Query: 241 TLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNND 300
           T+VSWKSRLRSWGY+PLFCSVQT+ GLDSLAYILRDQTTVIVGPSGVGKSSLINALR+N+
Sbjct: 241 TVVSWKSRLRSWGYDPLFCSVQTRIGLDSLAYILRDQTTVIVGPSGVGKSSLINALRSNN 300

Query: 301 RGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF 360
           R  DAVEGDNWFDPI  SKWFEDQRVAEVSTRSGRGKHTTRHVSLLPL GGGYLADTPGF
Sbjct: 301 RAADAVEGDNWFDPIRDSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLMGGGYLADTPGF 360

Query: 361 NQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQL 420
           NQPSLMKITKQSLAQAFPEI+  LLA+EPA+CSFNDCLHLGEPGCVI GDWERYQYYFQL
Sbjct: 361 NQPSLMKITKQSLAQAFPEIRNKLLASEPAKCSFNDCLHLGEPGCVINGDWERYQYYFQL 420

Query: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480
           LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL
Sbjct: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480

Query: 481 DELEDEDEDDSLDKEDPILKAMRNEQQ 506
           DELEDED DDSL+++DPIL+AMRNE +
Sbjct: 481 DELEDED-DDSLEEDDPILRAMRNENK 506

BLAST of MS000977 vs. NCBI nr
Match: KAG6585217.1 (Small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897.9 bits (2319), Expect = 4.0e-257
Identity = 461/507 (90.93%), Postives = 483/507 (95.27%), Query Frame = 0

Query: 1   MAIASISMLRHHTA--AATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNL 60
           MAIASISMLRHH+   AA AS+P FGNVLRR FNVRC S+VAKQHNPNNVSNKPPPSKNL
Sbjct: 64  MAIASISMLRHHSTSIAAIASVPGFGNVLRRCFNVRCLSIVAKQHNPNNVSNKPPPSKNL 123

Query: 61  LRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQD 120
           LRAKHTFK+YSSLAP LSPQD PPLSESQAIGTVAAAQANFMRVIVQS PSQLSDSSN D
Sbjct: 124 LRAKHTFKNYSSLAPVLSPQDIPPLSESQAIGTVAAAQANFMRVIVQSPPSQLSDSSNND 183

Query: 121 SEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180
           SE SGSLEGDS +SSRLG+ELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF
Sbjct: 184 SERSGSLEGDSLDSSRLGIELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 243

Query: 181 QRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEE 240
           QRSSEILDPPVANVDHLLVLFS++QPKLEPFTLTRFL+EAESTGIPLTLGLNKLELV EE
Sbjct: 244 QRSSEILDPPVANVDHLLVLFSIEQPKLEPFTLTRFLVEAESTGIPLTLGLNKLELVDEE 303

Query: 241 TLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNND 300
           T+VSWKSRLRSWGY+PLFCSVQT+TGLDSLAYILRDQTTVIVGPSGVGKSSLINALR+N+
Sbjct: 304 TVVSWKSRLRSWGYDPLFCSVQTRTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRSNN 363

Query: 301 RGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF 360
           R  DAVEGDNWFDPI  SKWFEDQRVAEVSTRSGRGKHTTRHVSLLPL GGGYLADTPGF
Sbjct: 364 RAADAVEGDNWFDPIRDSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLMGGGYLADTPGF 423

Query: 361 NQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQL 420
           NQPSLMKITKQSLAQAFPEI+  LLA+EPA+CSFNDCLHLGEPGCVI GDWERYQYYFQL
Sbjct: 424 NQPSLMKITKQSLAQAFPEIRNKLLASEPAKCSFNDCLHLGEPGCVINGDWERYQYYFQL 483

Query: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480
           LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL
Sbjct: 484 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 543

Query: 481 DELEDEDEDDSLDKEDPILKAMRNEQQ 506
           DELEDED DDSL+++DPIL+AMRNE +
Sbjct: 544 DELEDED-DDSLEEDDPILRAMRNENK 569

BLAST of MS000977 vs. ExPASy Swiss-Prot
Match: Q4V399 (Small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=rsgA PE=2 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 8.1e-176
Identity = 317/449 (70.60%), Postives = 369/449 (82.18%), Query Frame = 0

Query: 58  LLRAKHTFKDY-SSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSN 117
           +LRAKH  K+Y SSL+P LSP+  P L ESQAIGTVA AQANFMRVIVQ + + ++    
Sbjct: 1   MLRAKHIGKNYSSSLSPVLSPEHKPSLLESQAIGTVATAQANFMRVIVQDVANSVTSD-- 60

Query: 118 QDSEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIEN 177
                      D ++SS+ GVELLCVV+AVLKKI+RRVLVGDKV+VGSIDWVDRRGMIEN
Sbjct: 61  -----------DDNDSSKTGVELLCVVRAVLKKIRRRVLVGDKVLVGSIDWVDRRGMIEN 120

Query: 178 VFQRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVA 237
           VF R SEILDPPVANVDHLLVLFS+DQPKLEPFTLTRFL+EAEST IPLTL LNK EL++
Sbjct: 121 VFHRRSEILDPPVANVDHLLVLFSLDQPKLEPFTLTRFLVEAESTRIPLTLALNKTELIS 180

Query: 238 EETLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRN 297
           EE L +WK RLR W YEPLFCSV TK GLD +A++LRDQT+VIVGPSGVGKSSLIN LR+
Sbjct: 181 EEELETWKIRLRGWNYEPLFCSVGTKDGLDDIAFVLRDQTSVIVGPSGVGKSSLINVLRS 240

Query: 298 NDRGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTP 357
           N  G   VE +NWF+P+LG+KWF+DQRV EVS+RSGRGKHTTR+VSLLP+S GGYLADTP
Sbjct: 241 N-HGGGVVEDENWFEPMLGNKWFDDQRVGEVSSRSGRGKHTTRNVSLLPVSEGGYLADTP 300

Query: 358 GFNQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYF 417
           GFNQPSL+K+TK SLA  FPEI+ M+   E  +C F DCLH+GEPGCV+KGDWERY YY 
Sbjct: 301 GFNQPSLLKVTKHSLAHCFPEIRNMI---ESEKCGFRDCLHIGEPGCVVKGDWERYPYYL 360

Query: 418 QLLDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILN 477
           QLLDEIRIREEFQLRTFGTKRE DVR KVGDMG + AEPRL  KKHRR+SRK+  Q++++
Sbjct: 361 QLLDEIRIREEFQLRTFGTKREDDVRYKVGDMGVKHAEPRLMPKKHRRESRKKTKQTMIS 420

Query: 478 ELDELEDEDEDDSLDKEDPILKAMRNEQQ 506
           ELDE EDED D  ++  DPI++A+ NE +
Sbjct: 421 ELDEFEDEDSDLYIE-NDPIVQAIENENK 431

BLAST of MS000977 vs. ExPASy Swiss-Prot
Match: F4HTL8 (Small ribosomal subunit biogenesis GTPase RsgA 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g67460 PE=2 SV=2)

HSP 1 Score: 285.8 bits (730), Expect = 9.5e-76
Identity = 156/278 (56.12%), Postives = 194/278 (69.78%), Query Frame = 0

Query: 36  FSVVAKQHNPNNVSNKPPPSKNLLRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAA 95
           F    +Q NP NV+  P P+K +LR        S LAP L   + P L E QAIGT+A A
Sbjct: 36  FLSAIRQENP-NVTKNPHPNKTILR--------SFLAPVLPLDEKPNLVELQAIGTIATA 95

Query: 96  QANFMRVIVQSLPSQLSDSSNQDSEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVL 155
            A++MRVIVQ +P                 E D+ E  ++GVELLCVV+ +LKKI R VL
Sbjct: 96  LADYMRVIVQDVP-----------------ESDNGEDDKIGVELLCVVRKLLKKIGRTVL 155

Query: 156 VGDKVVVGSIDWVDRRGMIENVFQRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFL 215
           VGDKV+V  +DWVDRR  I NVF R SE+LDPPVANVDHL++LFS+DQPK++PFTLTRFL
Sbjct: 156 VGDKVLVDKVDWVDRRAKIINVFDRVSEVLDPPVANVDHLVILFSLDQPKIDPFTLTRFL 215

Query: 216 IEAESTGIPLTLGLNKLELVAEETLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQ 275
           +EAES GI +T+ LNK ELV +E + SWK RLRSW YEPLFCSV TK G+D +A+ LR+Q
Sbjct: 216 VEAESIGIRITVALNKCELVTQEEVESWKIRLRSWNYEPLFCSVGTKVGIDEIAFNLRNQ 275

Query: 276 TTVIVGPSGVGKSSLINALRNNDRGVDAVEGDNWFDPI 314
           T+VIVGPSGVGKSSLIN LR++  G   ++ +  F P+
Sbjct: 276 TSVIVGPSGVGKSSLINILRSSYGG--DIKHEEVFKPV 285

BLAST of MS000977 vs. ExPASy Swiss-Prot
Match: Q8YUA3 (Small ribosomal subunit biogenesis GTPase RsgA 1 OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=rsgA1 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.5e-68
Identity = 164/401 (40.90%), Postives = 228/401 (56.86%), Query Frame = 0

Query: 87  QAIGTVAAAQANFMRV-IVQSLPSQLSDSSNQDSEPSGSLEGDSSESSRLGVELLCVVKA 146
           Q +GTV A QANF +V + Q +  Q S  + ++      L         L + LLC  + 
Sbjct: 8   QLLGTVVAVQANFYKVQLDQEVREQGSRGAGEEVHLDSPL-----PLCPLSL-LLCTRRT 67

Query: 147 VLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQRSSEILDPPVANVDHLLVLFSMDQPK 206
            LKKI ++V+VGD+VVV   DW   RG I +V  R +++  PP+AN D +L++F++  P 
Sbjct: 68  RLKKIGQQVMVGDRVVVEEPDWAGGRGAIADVLSRQTQLDRPPIANADQILLVFAVADPP 127

Query: 207 LEPFTLTRFLIEAESTGIPLTLGLNKLELVAEETLVSWKSRLRSWGYEPLFCSVQTKTGL 266
           LEP+ L+RFL++AE+TG+ + L LNK +LV+ E       RL +WGY+PLF SV+ +  +
Sbjct: 128 LEPYQLSRFLVKAETTGLDVVLCLNKSDLVSPEIQQQISDRLLAWGYQPLFISVENQINI 187

Query: 267 DSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRGVDAVEGDNWFDPILGSKWFEDQRVA 326
           D +A  L ++ TV+ GPSGVGKSSLINAL  N                       + RV 
Sbjct: 188 DQIAKYLSNKITVVAGPSGVGKSSLINALIPN----------------------INLRVG 247

Query: 327 EVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQPSLMKITKQSLAQAFPEIQKMLLAN 386
           EVS +  RG+HTTRHV L  L  GG LADTPGFNQP  +  + + L   FPE ++ L   
Sbjct: 248 EVSGKLARGRHTTRHVELFELPNGGLLADTPGFNQPD-VDCSPEELVHYFPEARERLAI- 307

Query: 387 EPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLDEIRIREEFQLRTFGTKREGDVRIKV 446
             A C FNDC H  EP C ++GDWERY++Y + L +  I  + QL       E  +++K 
Sbjct: 308 --ASCRFNDCSHRDEPDCAVRGDWERYEHYLEFLAD-AIARQTQLYQ-QADPESTLKLKT 367

Query: 447 GDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDELEDEDE 487
              G  Q EP+LE KK+RR SR+   Q     L +L  E+E
Sbjct: 368 KGKGQSQYEPKLESKKYRRTSRRTQVQG----LQDLYQEEE 370

BLAST of MS000977 vs. ExPASy Swiss-Prot
Match: Q3MG79 (Small ribosomal subunit biogenesis GTPase RsgA OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=rsgA PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.3e-68
Identity = 164/401 (40.90%), Postives = 225/401 (56.11%), Query Frame = 0

Query: 87  QAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSEPSG-SLEGDSSESSRLGVELLCVVKA 146
           Q +GTV A QANF +V +        D     S  +G  +  DS         LLC  + 
Sbjct: 8   QLLGTVVAVQANFYKVQLD------QDVREPGSRGAGEEVHLDSPLPLYPLSLLLCTRRT 67

Query: 147 VLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQRSSEILDPPVANVDHLLVLFSMDQPK 206
            LKKI ++V+VGD+VVV   DW   RG I  V  R +++  PP+AN D +L++F++  P 
Sbjct: 68  RLKKIGQQVMVGDRVVVEEPDWAGGRGAIAEVLSRQTQLDRPPIANADQILLVFAVADPP 127

Query: 207 LEPFTLTRFLIEAESTGIPLTLGLNKLELVAEETLVSWKSRLRSWGYEPLFCSVQTKTGL 266
           LEP+ L+RFL++AE+TG+ + L LNK +LV+ E       RL +WGY+PLF SV+ +  +
Sbjct: 128 LEPYQLSRFLVKAETTGLDVVLCLNKSDLVSPEIQQQISDRLLAWGYQPLFISVEKQINI 187

Query: 267 DSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRGVDAVEGDNWFDPILGSKWFEDQRVA 326
           D +A  L ++ TV+ GPSGVGKSSLINAL  +                       + RV 
Sbjct: 188 DQIAKYLSNKITVVAGPSGVGKSSLINALIPD----------------------INLRVG 247

Query: 327 EVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQPSLMKITKQSLAQAFPEIQKMLLAN 386
           EVS +  RG+HTTRHV L  L  GG LADTPGFNQP  +  + + L   FPE ++ L   
Sbjct: 248 EVSGKLARGRHTTRHVELFELPNGGLLADTPGFNQPD-VDCSPEELVHYFPEARERLAV- 307

Query: 387 EPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLDEIRIREEFQLRTFGTKREGDVRIKV 446
             A C FNDCLH  EP C ++GDWERY++Y + L +  I  + QL       E  +++K 
Sbjct: 308 --ASCRFNDCLHRDEPDCAVRGDWERYEHYLEFLAD-AIARQTQLYQ-QADPESTLKLKT 367

Query: 447 GDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDELEDEDE 487
              G  Q EP+LE KK+RR SR+   Q     L +L  E+E
Sbjct: 368 KGKGQSQYEPKLESKKYRRTSRRTQVQG----LQDLYQEEE 370

BLAST of MS000977 vs. ExPASy Swiss-Prot
Match: B0JW21 (Small ribosomal subunit biogenesis GTPase RsgA OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=rsgA PE=3 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.2e-66
Identity = 152/401 (37.91%), Postives = 225/401 (56.11%), Query Frame = 0

Query: 90  GTVAAAQANFMRVIVQSLPSQLSDSSNQDSEPSGSLEGDSSESSRLGVELLCVVKAVLKK 149
           GTV A QANF +V +                          +S+ +G  LLC  +A L+K
Sbjct: 11  GTVVAVQANFYQVRL--------------------------DSAVMGENLLCTRRARLQK 70

Query: 150 IKRRVLVGDKVVVGSIDWVDRRGMIENVFQRSSEILDPPVANVDHLLVLFSMDQPKLEPF 209
           I + V+VGD+V V   ++ D++G I  V  RS+EI  P VAN++ +L++F++ +P L+P+
Sbjct: 71  IGQSVMVGDRVRVEEANFGDQQGAIAEVLPRSTEIDRPAVANIEQILLVFALAEPVLDPW 130

Query: 210 TLTRFLIEAESTGIPLTLGLNKLELVAEETLVSWKSRLRSWGYEPLFCSVQTKTGLDSLA 269
            ++RFL++AESTG+ + + +NK++L   E +  W  RL  WGY P F SV+   G ++L 
Sbjct: 131 LISRFLVKAESTGLEIAVCVNKIDLGEPEQIEVWGDRLAGWGYRPFFVSVEKNRGFEALL 190

Query: 270 YILRDQTTVIVGPSGVGKSSLINALRNNDRGVDAVEGDNWFDPILGSKWFEDQRVAEVST 329
             L  + T++ GPSGVGKSSLIN L      +  +                +QRV +VS 
Sbjct: 191 AQLNHKITLLAGPSGVGKSSLINCL------IPEI----------------NQRVGDVSG 250

Query: 330 RSGRGKHTTRHVSLLPLSGGGYLADTPGFNQPSLMKITKQSLAQAFPEIQKMLLANEPAR 389
           +  +G+HTTRHV L  L   G LAD+PGFNQP +     + L   FPE++  L       
Sbjct: 251 KLQKGRHTTRHVQLFALPNRGLLADSPGFNQPDI-NCLPEELTFYFPEVRARLAL---GN 310

Query: 390 CSFNDCLHLGEPGCVIKGDWERYQYYFQLLDEIRIREEFQLRTFGTKREGDVRIKVGDMG 449
           C FNDC H  EP CV++GDWERYQ+Y + L+E   RE+   +T  + +E  +++K+ + G
Sbjct: 311 CQFNDCTHRREPNCVVRGDWERYQHYLEFLEEAIAREQSLQKT--STKESSLKLKIKEAG 357

Query: 450 AQQAEPRLELKKHRRQSRKRVNQSILN-ELDELEDEDEDDS 490
            +  EP+L  KK+RR SR+  NQ     E   L+D   DDS
Sbjct: 371 QETYEPKLANKKYRRPSRRGKNQDQERYENKTLQDIYNDDS 357

BLAST of MS000977 vs. ExPASy TrEMBL
Match: A0A6J1BS55 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005203 PE=3 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 6.6e-282
Identity = 503/505 (99.60%), Postives = 503/505 (99.60%), Query Frame = 0

Query: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60
           MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR
Sbjct: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60

Query: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120
           AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE
Sbjct: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120

Query: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180
           PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR
Sbjct: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180

Query: 181 SSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEETL 240
           SSEILDPPVANVDHLLVLFSMDQPKLE FTLTRFLIEAESTGIPLTLGLNKLELV EETL
Sbjct: 181 SSEILDPPVANVDHLLVLFSMDQPKLESFTLTRFLIEAESTGIPLTLGLNKLELVDEETL 240

Query: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300
           VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG
Sbjct: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300

Query: 301 VDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQ 360
           VDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQ
Sbjct: 301 VDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFNQ 360

Query: 361 PSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLD 420
           PSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLD
Sbjct: 361 PSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLLD 420

Query: 421 EIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDE 480
           EIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDE
Sbjct: 421 EIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELDE 480

Query: 481 LEDEDEDDSLDKEDPILKAMRNEQQ 506
           LEDEDEDDSLDKEDPILKAMRNEQQ
Sbjct: 481 LEDEDEDDSLDKEDPILKAMRNEQQ 505

BLAST of MS000977 vs. ExPASy TrEMBL
Match: A0A6J1BSR6 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005203 PE=3 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.6e-280
Identity = 503/506 (99.41%), Postives = 503/506 (99.41%), Query Frame = 0

Query: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60
           MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR
Sbjct: 1   MAIASISMLRHHTAAATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNLLR 60

Query: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120
           AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE
Sbjct: 61  AKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQDSE 120

Query: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180
           PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR
Sbjct: 121 PSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVFQR 180

Query: 181 SSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEETL 240
           SSEILDPPVANVDHLLVLFSMDQPKLE FTLTRFLIEAESTGIPLTLGLNKLELV EETL
Sbjct: 181 SSEILDPPVANVDHLLVLFSMDQPKLESFTLTRFLIEAESTGIPLTLGLNKLELVDEETL 240

Query: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300
           VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG
Sbjct: 241 VSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNNDRG 300

Query: 301 VDAVEGDNWFDP-ILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFN 360
           VDAVEGDNWFDP ILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFN
Sbjct: 301 VDAVEGDNWFDPQILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGFN 360

Query: 361 QPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLL 420
           QPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLL
Sbjct: 361 QPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQLL 420

Query: 421 DEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELD 480
           DEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELD
Sbjct: 421 DEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNELD 480

Query: 481 ELEDEDEDDSLDKEDPILKAMRNEQQ 506
           ELEDEDEDDSLDKEDPILKAMRNEQQ
Sbjct: 481 ELEDEDEDDSLDKEDPILKAMRNEQQ 506

BLAST of MS000977 vs. ExPASy TrEMBL
Match: A0A6J1GIJ0 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111454543 PE=3 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 1.5e-257
Identity = 461/507 (90.93%), Postives = 483/507 (95.27%), Query Frame = 0

Query: 1   MAIASISMLRHHTA--AATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNL 60
           MAIASISMLRHH+   AA AS+P FGNVLRR FNVRC S+VAKQHNPNNVSNKPPPSKNL
Sbjct: 1   MAIASISMLRHHSTSIAAIASVPGFGNVLRRCFNVRCLSIVAKQHNPNNVSNKPPPSKNL 60

Query: 61  LRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQD 120
           LRAKHTFK+YSSLAP LSPQD PPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSN D
Sbjct: 61  LRAKHTFKNYSSLAPVLSPQDIPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNND 120

Query: 121 SEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180
           SE SGSLEGDS +SSRLG+ELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF
Sbjct: 121 SERSGSLEGDSLDSSRLGIELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180

Query: 181 QRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEE 240
           QRSSEILDPPVANVDHLLVLFS++QPKLEPFTLTRFL+EAESTGIPLTLGLNKLELV EE
Sbjct: 181 QRSSEILDPPVANVDHLLVLFSIEQPKLEPFTLTRFLVEAESTGIPLTLGLNKLELVDEE 240

Query: 241 TLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNND 300
           T+VSWKSRLRSWGY+PLFCSVQT+ GLDSLAYILRDQTTVIVGPSGVGKSSLINALR+N+
Sbjct: 241 TVVSWKSRLRSWGYDPLFCSVQTRIGLDSLAYILRDQTTVIVGPSGVGKSSLINALRSNN 300

Query: 301 RGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF 360
           R  DAVEGDNWFDPI  SKWFEDQRVAEVSTRSGRGKHTTRHVSLLPL GGGYLADTPGF
Sbjct: 301 RAADAVEGDNWFDPIRDSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLMGGGYLADTPGF 360

Query: 361 NQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQL 420
           NQPSLMKITKQSLAQAFPEI+  LLA+EPA+CSFNDCLHLGEPGCVI GDWERYQYYFQL
Sbjct: 361 NQPSLMKITKQSLAQAFPEIRNKLLASEPAKCSFNDCLHLGEPGCVINGDWERYQYYFQL 420

Query: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480
           LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL
Sbjct: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480

Query: 481 DELEDEDEDDSLDKEDPILKAMRNEQQ 506
           DELEDED DDSL+++DPIL+AMRNE +
Sbjct: 481 DELEDED-DDSLEEDDPILRAMRNENK 506

BLAST of MS000977 vs. ExPASy TrEMBL
Match: A0A6J1KPK6 (small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111496452 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.2e-256
Identity = 461/505 (91.29%), Postives = 481/505 (95.25%), Query Frame = 0

Query: 1   MAIASISMLRHHTA--AATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNL 60
           MAIASISMLRHH+   AA AS+PAFGNVLRR FNVRC SVVAKQHNPNNVSNKPPPSKNL
Sbjct: 1   MAIASISMLRHHSTSIAAIASVPAFGNVLRRCFNVRCLSVVAKQHNPNNVSNKPPPSKNL 60

Query: 61  LRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQD 120
           LRAKHTFK+YSSLAP LSPQD PPLSESQAIGTVAAAQANFMRVIVQSLPS+LS SSN D
Sbjct: 61  LRAKHTFKNYSSLAPVLSPQDIPPLSESQAIGTVAAAQANFMRVIVQSLPSRLSVSSNND 120

Query: 121 SEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180
           SE SGS EGDSS+SSRLG+ELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF
Sbjct: 121 SELSGSSEGDSSDSSRLGIELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180

Query: 181 QRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEE 240
           QRSSEILDPPVANVDHLLVLFS+DQPKLEPFTLTRFL+EAESTGIPLTLGLNKLELV EE
Sbjct: 181 QRSSEILDPPVANVDHLLVLFSIDQPKLEPFTLTRFLVEAESTGIPLTLGLNKLELVDEE 240

Query: 241 TLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNND 300
           T+VSWKSRLRSWGY+PLFCSVQT+ GLDSLAYILRDQTTVIVGPSGVGKSSLINALR+N+
Sbjct: 241 TVVSWKSRLRSWGYDPLFCSVQTRIGLDSLAYILRDQTTVIVGPSGVGKSSLINALRSNN 300

Query: 301 RGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF 360
           R  DAVEGDNWFDPI  SKWFEDQRVAEVSTRSGRGKHTTRHVSLLPL GGGYLADTPGF
Sbjct: 301 RAADAVEGDNWFDPIRDSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLMGGGYLADTPGF 360

Query: 361 NQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQL 420
           NQPSLMKITKQSLAQAFPEI+  LLA+EPA+CSFNDCLHLGEPGCVI GDWERYQYYFQL
Sbjct: 361 NQPSLMKITKQSLAQAFPEIRNKLLASEPAKCSFNDCLHLGEPGCVINGDWERYQYYFQL 420

Query: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480
           LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL
Sbjct: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480

Query: 481 DELEDEDEDDSLDKEDPILKAMRNE 504
           DELEDED DDS +++DPIL+AMRNE
Sbjct: 481 DELEDED-DDSSEEDDPILRAMRNE 504

BLAST of MS000977 vs. ExPASy TrEMBL
Match: A0A0A0KGV4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G365180 PE=3 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 4.5e-254
Identity = 456/507 (89.94%), Postives = 479/507 (94.48%), Query Frame = 0

Query: 1   MAIASISMLRHHTA--AATASIPAFGNVLRRFFNVRCFSVVAKQHNPNNVSNKPPPSKNL 60
           MAIASISMLRHHTA  AAT+SIP FGNVLRR FN RCFS+VAKQHNPNNVSNKPPPSKNL
Sbjct: 1   MAIASISMLRHHTAAIAATSSIPVFGNVLRRVFNFRCFSIVAKQHNPNNVSNKPPPSKNL 60

Query: 61  LRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSNQD 120
           LRAKHTFKDYSSLAP LSPQDNPPLSESQAIGTVAAAQANFMRVIVQ+LPSQ S S N D
Sbjct: 61  LRAKHTFKDYSSLAPVLSPQDNPPLSESQAIGTVAAAQANFMRVIVQTLPSQFSQSPNDD 120

Query: 121 SEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180
            E SGSLE DSS+SS LGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF
Sbjct: 121 LERSGSLEDDSSKSSMLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIENVF 180

Query: 181 QRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVAEE 240
           QRSSEILDPPVANVDHLLVLFSMDQP+LEPFTLTRFL+EAESTGIPLTLGLNKLELV EE
Sbjct: 181 QRSSEILDPPVANVDHLLVLFSMDQPRLEPFTLTRFLVEAESTGIPLTLGLNKLELVDEE 240

Query: 241 TLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRNND 300
           TLVSWKSRLRSWGY+PLFCSVQT  GLDSLA+ILRDQTTVIVGPSGVGKSSLINALRN++
Sbjct: 241 TLVSWKSRLRSWGYDPLFCSVQTGAGLDSLAFILRDQTTVIVGPSGVGKSSLINALRNSN 300

Query: 301 RGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF 360
           R  DA + DNWFDPILGSKWFE+QRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF
Sbjct: 301 RATDASDTDNWFDPILGSKWFEEQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTPGF 360

Query: 361 NQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYFQL 420
           NQPSLMKITKQSLAQAFPEIQKML A+EPA+CSFNDCLHLGEPGCVIKGDWERYQYYFQL
Sbjct: 361 NQPSLMKITKQSLAQAFPEIQKMLHASEPAKCSFNDCLHLGEPGCVIKGDWERYQYYFQL 420

Query: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILNEL 480
           LDEIRIREEFQLRTFGTKREGDVRIKVG+MG +QAEPRLELKKHRRQSRKRVNQSIL+EL
Sbjct: 421 LDEIRIREEFQLRTFGTKREGDVRIKVGEMGVKQAEPRLELKKHRRQSRKRVNQSILDEL 480

Query: 481 DELEDEDEDDSLDKEDPILKAMRNEQQ 506
           DELEDED+   L++ +PIL+AM+NE Q
Sbjct: 481 DELEDEDD---LEETNPILRAMKNENQ 504

BLAST of MS000977 vs. TAIR 10
Match: AT1G67440.1 (Minichromosome maintenance (MCM2/3/5) family protein )

HSP 1 Score: 618.2 bits (1593), Expect = 5.7e-177
Identity = 317/449 (70.60%), Postives = 369/449 (82.18%), Query Frame = 0

Query: 58  LLRAKHTFKDY-SSLAPALSPQDNPPLSESQAIGTVAAAQANFMRVIVQSLPSQLSDSSN 117
           +LRAKH  K+Y SSL+P LSP+  P L ESQAIGTVA AQANFMRVIVQ + + ++    
Sbjct: 1   MLRAKHIGKNYSSSLSPVLSPEHKPSLLESQAIGTVATAQANFMRVIVQDVANSVTSD-- 60

Query: 118 QDSEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVLVGDKVVVGSIDWVDRRGMIEN 177
                      D ++SS+ GVELLCVV+AVLKKI+RRVLVGDKV+VGSIDWVDRRGMIEN
Sbjct: 61  -----------DDNDSSKTGVELLCVVRAVLKKIRRRVLVGDKVLVGSIDWVDRRGMIEN 120

Query: 178 VFQRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFLIEAESTGIPLTLGLNKLELVA 237
           VF R SEILDPPVANVDHLLVLFS+DQPKLEPFTLTRFL+EAEST IPLTL LNK EL++
Sbjct: 121 VFHRRSEILDPPVANVDHLLVLFSLDQPKLEPFTLTRFLVEAESTRIPLTLALNKTELIS 180

Query: 238 EETLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQTTVIVGPSGVGKSSLINALRN 297
           EE L +WK RLR W YEPLFCSV TK GLD +A++LRDQT+VIVGPSGVGKSSLIN LR+
Sbjct: 181 EEELETWKIRLRGWNYEPLFCSVGTKDGLDDIAFVLRDQTSVIVGPSGVGKSSLINVLRS 240

Query: 298 NDRGVDAVEGDNWFDPILGSKWFEDQRVAEVSTRSGRGKHTTRHVSLLPLSGGGYLADTP 357
           N  G   VE +NWF+P+LG+KWF+DQRV EVS+RSGRGKHTTR+VSLLP+S GGYLADTP
Sbjct: 241 N-HGGGVVEDENWFEPMLGNKWFDDQRVGEVSSRSGRGKHTTRNVSLLPVSEGGYLADTP 300

Query: 358 GFNQPSLMKITKQSLAQAFPEIQKMLLANEPARCSFNDCLHLGEPGCVIKGDWERYQYYF 417
           GFNQPSL+K+TK SLA  FPEI+ M+   E  +C F DCLH+GEPGCV+KGDWERY YY 
Sbjct: 301 GFNQPSLLKVTKHSLAHCFPEIRNMI---ESEKCGFRDCLHIGEPGCVVKGDWERYPYYL 360

Query: 418 QLLDEIRIREEFQLRTFGTKREGDVRIKVGDMGAQQAEPRLELKKHRRQSRKRVNQSILN 477
           QLLDEIRIREEFQLRTFGTKRE DVR KVGDMG + AEPRL  KKHRR+SRK+  Q++++
Sbjct: 361 QLLDEIRIREEFQLRTFGTKREDDVRYKVGDMGVKHAEPRLMPKKHRRESRKKTKQTMIS 420

Query: 478 ELDELEDEDEDDSLDKEDPILKAMRNEQQ 506
           ELDE EDED D  ++  DPI++A+ NE +
Sbjct: 421 ELDEFEDEDSDLYIE-NDPIVQAIENENK 431

BLAST of MS000977 vs. TAIR 10
Match: AT1G67460.1 (Minichromosome maintenance (MCM2/3/5) family protein )

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-76
Identity = 154/265 (58.11%), Postives = 188/265 (70.94%), Query Frame = 0

Query: 36  FSVVAKQHNPNNVSNKPPPSKNLLRAKHTFKDYSSLAPALSPQDNPPLSESQAIGTVAAA 95
           F    +Q NP NV+  P P+K +LR        S LAP L   + P L E QAIGT+A A
Sbjct: 36  FLSAIRQENP-NVTKNPHPNKTILR--------SFLAPVLPLDEKPNLVELQAIGTIATA 95

Query: 96  QANFMRVIVQSLPSQLSDSSNQDSEPSGSLEGDSSESSRLGVELLCVVKAVLKKIKRRVL 155
            A++MRVIVQ +P                 E D+ E  ++GVELLCVV+ +LKKI R VL
Sbjct: 96  LADYMRVIVQDVP-----------------ESDNGEDDKIGVELLCVVRKLLKKIGRTVL 155

Query: 156 VGDKVVVGSIDWVDRRGMIENVFQRSSEILDPPVANVDHLLVLFSMDQPKLEPFTLTRFL 215
           VGDKV+V  +DWVDRR  I NVF R SE+LDPPVANVDHL++LFS+DQPK++PFTLTRFL
Sbjct: 156 VGDKVLVDKVDWVDRRAKIINVFDRVSEVLDPPVANVDHLVILFSLDQPKIDPFTLTRFL 215

Query: 216 IEAESTGIPLTLGLNKLELVAEETLVSWKSRLRSWGYEPLFCSVQTKTGLDSLAYILRDQ 275
           +EAES GI +T+ LNK ELV +E + SWK RLRSW YEPLFCSV TK G+D +A+ LR+Q
Sbjct: 216 VEAESIGIRITVALNKCELVTQEEVESWKIRLRSWNYEPLFCSVGTKVGIDEIAFNLRNQ 274

Query: 276 TTVIVGPSGVGKSSLINALRNNDRG 301
           T+VIVGPSGVGKSSLIN LR++  G
Sbjct: 276 TSVIVGPSGVGKSSLINILRSSYGG 274

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132325.11.4e-28199.60small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X2 [Momo... [more]
XP_022132324.13.4e-28099.41small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X1 [Momo... [more]
XP_023536955.12.8e-25891.12small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial [Cucurbita pepo ... [more]
XP_022951811.13.1e-25790.93small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial-like [Cucurbita ... [more]
KAG6585217.14.0e-25790.93Small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial, partial [Cucurb... [more]
Match NameE-valueIdentityDescription
Q4V3998.1e-17670.60Small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial OS=Arabidopsis t... [more]
F4HTL89.5e-7656.12Small ribosomal subunit biogenesis GTPase RsgA 2, mitochondrial OS=Arabidopsis t... [more]
Q8YUA32.5e-6840.90Small ribosomal subunit biogenesis GTPase RsgA 1 OS=Nostoc sp. (strain PCC 7120 ... [more]
Q3MG797.3e-6840.90Small ribosomal subunit biogenesis GTPase RsgA OS=Trichormus variabilis (strain ... [more]
B0JW215.2e-6637.91Small ribosomal subunit biogenesis GTPase RsgA OS=Microcystis aeruginosa (strain... [more]
Match NameE-valueIdentityDescription
A0A6J1BS556.6e-28299.60small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X2 OS=Mo... [more]
A0A6J1BSR61.6e-28099.41small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial isoform X1 OS=Mo... [more]
A0A6J1GIJ01.5e-25790.93small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial-like OS=Cucurbit... [more]
A0A6J1KPK62.2e-25691.29small ribosomal subunit biogenesis GTPase RsgA 1, mitochondrial OS=Cucurbita max... [more]
A0A0A0KGV44.5e-25489.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G365180 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G67440.15.7e-17770.60Minichromosome maintenance (MCM2/3/5) family protein [more]
AT1G67460.11.1e-7658.11Minichromosome maintenance (MCM2/3/5) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 464..484
NoneNo IPR availableGENE3D1.10.40.50Probable gtpase engc; domain 3coord: 365..427
e-value: 1.2E-13
score: 52.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 477..491
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 459..505
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 110..130
NoneNo IPR availablePANTHERPTHR32120:SF11SMALL RIBOSOMAL SUBUNIT BIOGENESIS GTPASE RSGA 1, MITOCHONDRIAL-RELATEDcoord: 77..485
IPR004881Ribosome biogenesis GTPase RsgATIGRFAMTIGR00157TIGR00157coord: 154..296
e-value: 4.1E-34
score: 116.1
IPR004881Ribosome biogenesis GTPase RsgAPANTHERPTHR32120SMALL RIBOSOMAL SUBUNIT BIOGENESIS GTPASE RSGAcoord: 77..485
IPR004881Ribosome biogenesis GTPase RsgAHAMAPMF_01820GTPase_RsgAcoord: 88..426
score: 29.631926
IPR004881Ribosome biogenesis GTPase RsgACDDcd01854YjeQ_EngCcoord: 190..420
e-value: 2.83119E-80
score: 247.311
IPR010914RsgA GTPase domainPFAMPF03193RsgA_GTPasecoord: 177..363
e-value: 5.4E-57
score: 192.3
IPR010914RsgA GTPase domainPROSITEPS50936ENGC_GTPASEcoord: 190..359
score: 40.410469
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 176..363
e-value: 5.2E-48
score: 164.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 184..425
IPR030378Circularly permuted (CP)-type guanine nucleotide-binding (G) domainPROSITEPS51721G_CPcoord: 181..361
score: 48.537266
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 91..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000977.1MS000977.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding