MS000973 (gene) Bitter gourd (TR) v1

Overview


Name	MS000973
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Proteasome subunit beta
Location	scaffold36: 576370 .. 579030 (+)
RNA-Seq Expression	MS000973
Synteny	MS000973

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCTTGATACCAGTGGTCTTCAAACGACTGCTCCGCTGTTTGGGCCAAAAATGGAGATTTTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAATGATGTAATTTGTAAAGCATTTATCATACGGCTTCTGGGCTGCGTACTGTTCTGTATTATTGTTTTTTCTGACTTTGATTTTCTCCGTATTTCAGTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCGGCAAAAGGAACAACCACTCTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCCGATTCCCGTGCTAGCATGGGAGGCTATATATGTATGTTTACTTAATTACATTTGTTCTTCAAACAAATTTGAAATTCATTTTCTAGATGTTTTGTGGGTCGAATACTTCAACCTTTTTAGTGATTACGAGGTTAATTTTCTTCCATTATGTGTCCATATATGATATAAATGAGGTGCTATATGCAGCATGGATGAATTTATTCACTATTTCCCGTTTAGCTTTATGCTGACTTGTCTTCCCCTCCCTCCAAGTTTTGTAAGTTGTCGTGTTGTTATTTTTGCACCTTTCTTCCTTTTCAACTCAATATTCGTTTTTGAATTTTTTCAGCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAATTTTGGCATAGAAACCTCGGTGTCAAGGTAATTGGCTGATATGCAATTACTTGTAGTTGTAAGACTATCAACATTTTAGTAAGAATGGGTTACCTTATACTACTATCATTGTGAACTCGATAGGTACGCTTGCTTAATATATGAGGTTAATCTTGATTAATTGCCTTCACTTTTAGAGTTTTCCAGTCTCAACAACTCAACTCAACATGTAAATGTGCAATCAGATATTCATCCTTCCCTAATTGGATTAATTGTGCCTTGCCAATGTTGATATTTTGGATTTTTTTAATCTTGTGATTAGCTGATCATAGTGTTTAACCTGATTTAGTGCCGCCGACATGAACTAGCAAATAAACGCCGAATTTCAGTCGCAGGAGCATCAAAGTTATTGGCTAACATTCTGTACTCCTATCGCGGAATGGGTCTCTCTGTCGGGACTATGATTGCTGGATGGGATGAAACAGTAATTACATATTTATTTATTTTCATTTTCCCACTTTATGGTGATCTATAATTTACCTAATTCTGTGCTTTGTAGGGTCCTGGTCTATATTACGTAGACAGCGAGGGGGGAAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGGTAAGTATGATTCTCAATTATCATGCTGTCTTTGAAAATTTCTCAATTCAGTAATTTTGCAATTGAACCTATCTTGCCAAGATAAATCATGTAGTCTTCCCACATGTCTGTGAAATGATGGCAAAAAACATGATCTTATGTTTTGTTTGTGCAAGGGTGACATGCTGGTTATATATATATATATACATATTATATCGTTAGAATCCCAAATACAGTAAATGGAACTAGATTACTATATTGTTATATCAAAGAGATAGTTACAATATCAATAGCCCTTCGAGAGGGCTATCTCTCTCCCAAGAACTCTCTCAAGTTTCTTCACTATAAACTATTCCCAAAGTTATCCTTCTCCTCTCCCTACTACTATTTATAACACCCCTAATTAATACCACAATATCCCCCAATAATATACTAATAATACTCCCAAACTATCTCTACTAGGACTCTCATATTACCCCGCCCTTCAAAGACAACCATAATACCCCCATATTAATATTCTCAAACTAGTAAGGACTGTCTCTCTCACACACATATATATATTTGAAACATGAAAATTTCTCTGATTTGATATATATTGAAAGTTACACTGAGGTAGTACAAAAGATAGAAACAAAGAGAACTTTTAACTCCCCCACTAAACTAAATATACAATTAATTCCACATCCCTTCTCTACAATACCTCTCCCACAGCCTTATGTAAAAAGAAGCTCAGCAAAAAGCCTCCCAATTAGAATTAATTGAAAGCTATATCTCAACTTGTAATTGTGATTATATTTCTCGGTCTTTCCTGTTGTCGAGCTAACGTACTGCTTTGCGTGATGATGTTGGTACAGACATTTCTTCGATATTCTTTTCTCTTACCTCTCTTCTTTCCCCTCTTTTTGAGACAATTGTCCATCATTTCATCTAATGTGAGTTTATCTATGATTTTATGTATGTTAATTCATGAATACCCTATTGTGTGTGCTACTGAGCAGATATAGGTATGACTTATCTGTTGAAGAAGCTGCTGAGCTGGCCAGACGAGCCATTTATCATGCAACTTTTCGAGATGGAGCAAGTGGTGGAGTTGCCAGCGGTATCCTTCTGTCTAGATATTTATCTCTCTTTGTAATACACACACATTTATGTGCTTGTGCAAATTCCCCTCTTCTCACTCATGATTTGAATGTCACTGATCAGTTTATTATGTGGGACCAAATGGATGGAAGAAGCTCTCTGGCGATGATGTGGGAGAACTTCACTACAACTACTATCCAGTGACACCAAGTACAGTGGATCAGGAAATGGTTGAAGTAACCGGTGCT

mRNA sequence

ATGAAGCTTGATACCAGTGGTCTTCAAACGACTGCTCCGCTGTTTGGGCCAAAAATGGAGATTTTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAATGATTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCGGCAAAAGGAACAACCACTCTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCCGATTCCCGTGCTAGCATGGGAGGCTATATATCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAATTTTGGCATAGAAACCTCGGTGTCAAGTGCCGCCGACATGAACTAGCAAATAAACGCCGAATTTCAGTCGCAGGAGCATCAAAGTTATTGGCTAACATTCTGTACTCCTATCGCGGAATGGGTCTCTCTGTCGGGACTATGATTGCTGGATGGGATGAAACAGGTCCTGGTCTATATTACGTAGACAGCGAGGGGGGAAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGATATAGGTATGACTTATCTGTTGAAGAAGCTGCTGAGCTGGCCAGACGAGCCATTTATCATGCAACTTTTCGAGATGGAGCAAGTGGTGGAGTTGCCAGCGGTATCCTTCTGTCTAGATATTTATCTCTCTTTGTAATACACACACATTTATGTGCTTGTGCAAATTCCCCTCTTCTCACTCATGATTTGAATGTCTCAGTTTATTATGTGGGACCAAATGGATGGAAGAAGCTCTCTGGCGATGATGTGGGAGAACTTCACTACAACTACTATCCAGTGACACCAAGTACAGTGGATCAGGAAATGGTTGAAGTAACCGGTGCT

Coding sequence (CDS)

ATGAAGCTTGATACCAGTGGTCTTCAAACGACTGCTCCGCTGTTTGGGCCAAAAATGGAGATTTTGGAAGGGTTTTCAGCTGCTCCATCCTTTGAGATCCCTAATTCCAATGATTTTGATGGCTTTCAGAAAGAAGCCATTCAGATGGTGAAGCCGGCAAAAGGAACAACCACTCTGGCTTTCATTTTTAAGGAAGGAGTCATGGTTGCTGCCGATTCCCGTGCTAGCATGGGAGGCTATATATCATCACAATCTGTGAAAAAAATTATTGAAATCAATCCTTACATGCTTGGCACAATGGCTGGAGGTGCTGCTGATTGCCAATTTTGGCATAGAAACCTCGGTGTCAAGTGCCGCCGACATGAACTAGCAAATAAACGCCGAATTTCAGTCGCAGGAGCATCAAAGTTATTGGCTAACATTCTGTACTCCTATCGCGGAATGGGTCTCTCTGTCGGGACTATGATTGCTGGATGGGATGAAACAGGTCCTGGTCTATATTACGTAGACAGCGAGGGGGGAAGGCTGAAAGGAACAAGATTTTCTGTTGGATCTGGTTCACCTTATGCCTATGGTGTCTTGGATAGTGGATATAGGTATGACTTATCTGTTGAAGAAGCTGCTGAGCTGGCCAGACGAGCCATTTATCATGCAACTTTTCGAGATGGAGCAAGTGGTGGAGTTGCCAGCGGTATCCTTCTGTCTAGATATTTATCTCTCTTTGTAATACACACACATTTATGTGCTTGTGCAAATTCCCCTCTTCTCACTCATGATTTGAATGTCTCAGTTTATTATGTGGGACCAAATGGATGGAAGAAGCTCTCTGGCGATGATGTGGGAGAACTTCACTACAACTACTATCCAGTGACACCAAGTACAGTGGATCAGGAAATGGTTGAAGTAACCGGTGCT

Protein sequence

MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSLFVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMVEVTGA

Homology

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BLAST of MS000973 vs. NCBI nr
Match: XP_022131817.1 (proteasome subunit beta type-5 [Momordica charantia] >XP_022131818.1 proteasome subunit beta type-5 [Momordica charantia])

HSP 1 Score: 535.4 bits (1378), Expect = 3.2e-148
Identity = 272/305 (89.18%), Postives = 272/305 (89.18%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 272

Query: 301 EVTGA 306
           EVTGA
Sbjct: 301 EVTGA 272

[Close]

BLAST of MS000973 vs. NCBI nr
Match: XP_038883961.1 (proteasome subunit beta type-5 isoform X1 [Benincasa hispida])

HSP 1 Score: 523.1 bits (1346), Expect = 1.6e-144
Identity = 264/305 (86.56%), Postives = 269/305 (88.20%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMA 272

Query: 301 EVTGA 306
           EVT A
Sbjct: 301 EVTAA 272

[Close]

BLAST of MS000973 vs. NCBI nr
Match: XP_022961789.1 (proteasome subunit beta type-5-like [Cucurbita moschata] >XP_022997234.1 proteasome subunit beta type-5-like [Cucurbita maxima] >XP_022997235.1 proteasome subunit beta type-5-like [Cucurbita maxima])

HSP 1 Score: 522.7 bits (1345), Expect = 2.1e-144
Identity = 263/305 (86.23%), Postives = 269/305 (88.20%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME +EGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMA 272

Query: 301 EVTGA 306
           EVT A
Sbjct: 301 EVTAA 272

[Close]

BLAST of MS000973 vs. NCBI nr
Match: XP_008445492.1 (PREDICTED: proteasome subunit beta type-5 [Cucumis melo] >KAA0064739.1 proteasome subunit beta type-5 [Cucumis melo var. makuwa])

HSP 1 Score: 521.2 bits (1341), Expect = 6.2e-144
Identity = 262/305 (85.90%), Postives = 269/305 (88.20%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAP+FGP+ME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPMFGPRMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMT 272

Query: 301 EVTGA 306
           EVT A
Sbjct: 301 EVTAA 272

[Close]

BLAST of MS000973 vs. NCBI nr
Match: XP_023002169.1 (proteasome subunit beta type-5-B isoform X1 [Cucurbita maxima])

HSP 1 Score: 520.0 bits (1338), Expect = 1.4e-143
Identity = 262/301 (87.04%), Postives = 267/301 (88.70%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQT+APLFGPKMEILEGFSAAPSFE+PNS+DFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTSAPLFGPKMEILEGFSAAPSFEVPNSSDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMA 268

Query: 301 E 302
           E
Sbjct: 301 E 268

[Close]

BLAST of MS000973 vs. ExPASy Swiss-Prot
Match: O24361 (Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 3.1e-130
Identity = 229/305 (75.08%), Postives = 253/305 (82.95%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGL++TAP+F     + +G    PSF++PN  DFDGFQKEA+QMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLESTAPIFRRSDFVFDGLQMTPSFDLPNPTDFDGFQKEAVQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KCR 
Sbjct: 61  FIFKHGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCRL 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISV GASKLLANILY+YRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG R
Sbjct: 121 HELANKRRISVTGASKLLANILYNYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGMR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GY+YD++VEEA+ELARRAIYHAT+RDGASGGV            
Sbjct: 181 FSVGSGSPYAYGVLDNGYKYDMTVEEASELARRAIYHATYRDGASGGV------------ 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                VSVY+VGP+GWKK++GDDVG+LH+ YYPV P+TV+QEMV
Sbjct: 241 ---------------------VSVYHVGPDGWKKVTGDDVGDLHFQYYPVVPATVEQEMV 272

Query: 301 EVTGA 306
           EV GA
Sbjct: 301 EVVGA 272

[Close]

BLAST of MS000973 vs. ExPASy Swiss-Prot
Match: Q9LIP2 (Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 2.9e-128
Identity = 229/303 (75.58%), Postives = 252/303 (83.17%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGL+TT P+  FG   E+L+GFS+APSF++P + DFDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYL 240
            RFSVGSGSPYAYGVLDSGY++D+SVEEA+ELARR+IYHATFRDGASGGVA         
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVA--------- 240

Query: 241 SLFVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQE 300
                                   SVY+VGP GW KLSGDDVGELHY+YYPV P T +  
Sbjct: 241 ------------------------SVYHVGPQGWTKLSGDDVGELHYHYYPVAPITAEHV 270

Query: 301 MVE 302
           M E
Sbjct: 301 MEE 270

[Close]

BLAST of MS000973 vs. ExPASy Swiss-Prot
Match: O23717 (Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 2.1e-123
Identity = 222/305 (72.79%), Postives = 247/305 (80.98%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG +T+ P+  FG   ++L+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYL 240
            RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+IYHATFRDGASGGVA         
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVA--------- 240

Query: 241 SLFVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQE 300
                                   SVY+VGP GW KLSGDDVGELHY+YYPV P+T +Q 
Sbjct: 241 ------------------------SVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQV 272

Query: 301 MVEVT 304
           M E T
Sbjct: 301 MEEAT 272

[Close]

BLAST of MS000973 vs. ExPASy Swiss-Prot
Match: O55234 (Proteasome subunit beta type-5 OS=Mus musculus OX=10090 GN=Psmb5 PE=1 SV=3)

HSP 1 Score: 276.6 bits (706), Expect = 3.5e-73
Identity = 147/265 (55.47%), Postives = 179/265 (67.55%), Query Frame = 0


Query: 27  AAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSV 86
           AAPS+ +P        ++  I+M+    GTTTLAF F  GV+VAADSRA+ G YI+SQ+V
Sbjct: 42  AAPSWGVP--------EEPRIEML---HGTTTLAFKFLHGVIVAADSRATAGAYIASQTV 101

Query: 87  KKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYR 146
           KK+IEINPY+LGTMAGGAADC FW R L  +CR +EL NK RISVA ASKLLAN++Y Y+
Sbjct: 102 KKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYK 161

Query: 147 GMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEE 206
           GMGLS+GTMI GWD+ GPGLYYVDSEG R+ GT FSVGSGS YAYGV+D GY YDL VEE
Sbjct: 162 GMGLSMGTMICGWDKRGPGLYYVDSEGNRISGTAFSVGSGSVYAYGVMDRGYSYDLKVEE 221

Query: 207 AAELARRAIYHATFRDGASGGVASGILLSRYLSLFVIHTHLCACANSPLLTHDLNVSVYY 266
           A +LARRAIY AT+RD  SGG                                  V++Y+
Sbjct: 222 AYDLARRAIYQATYRDAYSGGA---------------------------------VNLYH 262

Query: 267 VGPNGWKKLSGDDVGELHYNYYPVT 292
           V  +GW ++S D+V +LH  Y  V+
Sbjct: 282 VREDGWIRVSSDNVADLHDKYSSVS 262

[Close]

BLAST of MS000973 vs. ExPASy Swiss-Prot
Match: P28074 (Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3)

HSP 1 Score: 275.8 bits (704), Expect = 5.9e-73
Identity = 145/266 (54.51%), Postives = 178/266 (66.92%), Query Frame = 0


Query: 27  AAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLAFIFKEGVMVAADSRASMGGYISSQSV 86
           AAP + +P        ++  I+M+    GTTTLAF F+ GV+VAADSRA+ G YI+SQ+V
Sbjct: 42  AAPGWGVP--------EEPGIEML---HGTTTLAFKFRHGVIVAADSRATAGAYIASQTV 101

Query: 87  KKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYR 146
           KK+IEINPY+LGTMAGGAADC FW R L  +CR +EL NK RISVA ASKLLAN++Y Y+
Sbjct: 102 KKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYK 161

Query: 147 GMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEE 206
           GMGLS+GTMI GWD+ GPGLYYVDSEG R+ G  FSVGSGS YAYGV+D GY YDL VE+
Sbjct: 162 GMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQ 221

Query: 207 AAELARRAIYHATFRDGASGGVASGILLSRYLSLFVIHTHLCACANSPLLTHDLNVSVYY 266
           A +LARRAIY AT+RD  SGG                                  V++Y+
Sbjct: 222 AYDLARRAIYQATYRDAYSGGA---------------------------------VNLYH 263

Query: 267 VGPNGWKKLSGDDVGELHYNYYPVTP 293
           V  +GW ++S D+V +LH  Y   TP
Sbjct: 282 VREDGWIRVSSDNVADLHEKYSGSTP 263

[Close]

BLAST of MS000973 vs. ExPASy TrEMBL
Match: A0A6J1BS30 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111004886 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.5e-148
Identity = 272/305 (89.18%), Postives = 272/305 (89.18%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 272

Query: 301 EVTGA 306
           EVTGA
Sbjct: 301 EVTGA 272

[Close]

BLAST of MS000973 vs. ExPASy TrEMBL
Match: A0A6J1KAW4 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111492198 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 263/305 (86.23%), Postives = 269/305 (88.20%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME +EGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMA 272

Query: 301 EVTGA 306
           EVT A
Sbjct: 301 EVTAA 272

[Close]

BLAST of MS000973 vs. ExPASy TrEMBL
Match: A0A6J1HF19 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462451 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.0e-144
Identity = 263/305 (86.23%), Postives = 269/305 (88.20%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAPLFGPKME +EGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPLFGPKMEFMEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMA 272

Query: 301 EVTGA 306
           EVT A
Sbjct: 301 EVTAA 272

[Close]

BLAST of MS000973 vs. ExPASy TrEMBL
Match: A0A5A7VGZ1 (Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00430 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 262/305 (85.90%), Postives = 269/305 (88.20%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAP+FGP+ME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPMFGPRMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMT 272

Query: 301 EVTGA 306
           EVT A
Sbjct: 301 EVTAA 272

[Close]

BLAST of MS000973 vs. ExPASy TrEMBL
Match: A0A1S3BCV3 (Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103488492 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.0e-144
Identity = 262/305 (85.90%), Postives = 269/305 (88.20%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPLFGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTTLA 60
           MKLDTSGLQTTAP+FGP+ME LEGF+AAPSFEIPNS+DFDGFQK+AIQMVKPAKGTTTLA
Sbjct: 1   MKLDTSGLQTTAPMFGPRMEALEGFAAAPSFEIPNSSDFDGFQKDAIQMVKPAKGTTTLA 60

Query: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120
           FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR
Sbjct: 61  FIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKCRR 120

Query: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180
           HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR
Sbjct: 121 HELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGTR 180

Query: 181 FSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYLSL 240
           FSVGSGSPYAYGVLD+GYRYDLSVEEAAELARRAIYHATFRDGASGGVA           
Sbjct: 181 FSVGSGSPYAYGVLDNGYRYDLSVEEAAELARRAIYHATFRDGASGGVA----------- 240

Query: 241 FVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQEMV 300
                                 SVYYVGPNGWKKLSGDDVGELHYNYYPVTP+TVDQEM 
Sbjct: 241 ----------------------SVYYVGPNGWKKLSGDDVGELHYNYYPVTPTTVDQEMT 272

Query: 301 EVTGA 306
           EVT A
Sbjct: 301 EVTAA 272

[Close]

BLAST of MS000973 vs. TAIR 10
Match: AT3G26340.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 459.5 bits (1181), Expect = 2.1e-129
Identity = 229/303 (75.58%), Postives = 252/303 (83.17%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGL+TT P+  FG   E+L+GFS+APSF++P + DFDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYL 240
            RFSVGSGSPYAYGVLDSGY++D+SVEEA+ELARR+IYHATFRDGASGGVA         
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVA--------- 240

Query: 241 SLFVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQE 300
                                   SVY+VGP GW KLSGDDVGELHY+YYPV P T +  
Sbjct: 241 ------------------------SVYHVGPQGWTKLSGDDVGELHYHYYPVAPITAEHV 270

Query: 301 MVE 302
           M E
Sbjct: 301 MEE 270

[Close]

BLAST of MS000973 vs. TAIR 10
Match: AT1G13060.1 (20S proteasome beta subunit E1 )

HSP 1 Score: 443.4 bits (1139), Expect = 1.5e-124
Identity = 222/305 (72.79%), Postives = 247/305 (80.98%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG +T+ P+  FG   ++L+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGVKC 120
           LAFIFK GVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLG+KC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKG 180
           R HELANKRRISV+GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EGGRLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGILLSRYL 240
            RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+IYHATFRDGASGGVA         
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVA--------- 240

Query: 241 SLFVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKKLSGDDVGELHYNYYPVTPSTVDQE 300
                                   SVY+VGP GW KLSGDDVGELHY+YYPV P+T +Q 
Sbjct: 241 ------------------------SVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQV 272

Query: 301 MVEVT 304
           M E T
Sbjct: 301 MEEAT 272

[Close]

BLAST of MS000973 vs. TAIR 10
Match: AT1G13060.2 (20S proteasome beta subunit E1 )

HSP 1 Score: 429.9 bits (1104), Expect = 1.7e-120
Identity = 222/329 (67.48%), Postives = 247/329 (75.08%), Query Frame = 0


Query: 1   MKLDTSGLQTTAPL--FGPKMEILEGFSAAPSFEIPNSNDFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG +T+ P+  FG   ++L+  S+ PSF++P + +FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFIFKEGVMVAADSRASMGGYIS------------------------SQSVKKIIEINP 120
           LAFIFK GVMVAADSRASMGGYIS                        SQSVKKIIEINP
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISVSSLKNSSKNMHPFDIVDFGRNTTSQSVKKIIEINP 120

Query: 121 YMLGTMAGGAADCQFWHRNLGVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGT 180
           YMLGTMAGGAADCQFWHRNLG+KCR HELANKRRISV+GASKLLAN+LYSYRGMGLSVGT
Sbjct: 121 YMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGT 180

Query: 181 MIAGWDETGPGLYYVDSEGGRLKGTRFSVGSGSPYAYGVLDSGYRYDLSVEEAAELARRA 240
           MIAGWDETGPGLYYVD+EGGRLKG RFSVGSGSPYAYGVLDSGY+YD+SVEEA+ELARR+
Sbjct: 181 MIAGWDETGPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRS 240

Query: 241 IYHATFRDGASGGVASGILLSRYLSLFVIHTHLCACANSPLLTHDLNVSVYYVGPNGWKK 300
           IYHATFRDGASGGVA                                 SVY+VGP GW K
Sbjct: 241 IYHATFRDGASGGVA---------------------------------SVYHVGPEGWTK 296

Query: 301 LSGDDVGELHYNYYPVTPSTVDQEMVEVT 304
           LSGDDVGELHY+YYPV P+T +Q M E T
Sbjct: 301 LSGDDVGELHYHYYPVAPATAEQVMEEAT 296

[Close]

BLAST of MS000973 vs. TAIR 10
Match: AT4G31300.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 3.5e-20
Identity = 56/182 (30.77%), Postives = 99/182 (54.40%), Query Frame = 0


Query: 55  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 114
           GTT +   +  GV++ ADSR S G Y+++++  KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 115 GVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 174
                +H + + +  +V  ++ L+  + Y+ + M L  G ++ GWD+   G  Y    GG
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNM-LQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 175 RLKGTRFSV-GSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGIL 234
            +    F++ GSGS Y YG  D  ++ +++ EEA +L  +A+  A  RDGASGGV   ++
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 235 LS 236
           ++
Sbjct: 192 IN 192

[Close]

BLAST of MS000973 vs. TAIR 10
Match: AT4G31300.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 3.5e-20
Identity = 55/182 (30.22%), Postives = 98/182 (53.85%), Query Frame = 0


Query: 55  GTTTLAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNL 114
           GTT +   +  GV++ ADSR S G Y+++++  KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 115 GVKCRRHELANKRRISVAGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGG 174
                +H + + +  +V  ++ L+  + Y+ +   L  G ++ GWD+   G  Y    GG
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKQNMLQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 175 RLKGTRFSV-GSGSPYAYGVLDSGYRYDLSVEEAAELARRAIYHATFRDGASGGVASGIL 234
            +    F++ GSGS Y YG  D  ++ +++ EEA +L  +A+  A  RDGASGGV   ++
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 235 LS 236
           ++
Sbjct: 192 IN 193

The following BLAST results are available for this feature:

BLAST of MS000973 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131817.1	3.2e-148	89.18	proteasome subunit beta type-5 [Momordica charantia] >XP_022131818.1 proteasome ...	[more]
XP_038883961.1	1.6e-144	86.56	proteasome subunit beta type-5 isoform X1 [Benincasa hispida]	[more]
XP_022961789.1	2.1e-144	86.23	proteasome subunit beta type-5-like [Cucurbita moschata] >XP_022997234.1 proteas...	[more]
XP_008445492.1	6.2e-144	85.90	PREDICTED: proteasome subunit beta type-5 [Cucumis melo] >KAA0064739.1 proteasom...	[more]
XP_023002169.1	1.4e-143	87.04	proteasome subunit beta type-5-B isoform X1 [Cucurbita maxima]	[more]

BLAST of MS000973 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24361	3.1e-130	75.08	Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1	[more]
Q9LIP2	2.9e-128	75.58	Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV...	[more]
O23717	2.1e-123	72.79	Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV...	[more]
O55234	3.5e-73	55.47	Proteasome subunit beta type-5 OS=Mus musculus OX=10090 GN=Psmb5 PE=1 SV=3	[more]
P28074	5.9e-73	54.51	Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3	[more]

BLAST of MS000973 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BS30	1.5e-148	89.18	Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111004886 PE=3 SV=1	[more]
A0A6J1KAW4	1.0e-144	86.23	Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111492198 PE=3 SV=1	[more]
A0A6J1HF19	1.0e-144	86.23	Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111462451 PE=3 SV=1	[more]
A0A5A7VGZ1	3.0e-144	85.90	Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A1S3BCV3	3.0e-144	85.90	Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103488492 PE=3 SV=1	[more]

BLAST of MS000973 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26340.1	2.1e-129	75.58	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT1G13060.1	1.5e-124	72.79	20S proteasome beta subunit E1	[more]
AT1G13060.2	1.7e-120	67.48	20S proteasome beta subunit E1	[more]
AT4G31300.1	3.5e-20	30.77	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G31300.2	3.5e-20	30.22	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000243	Peptidase T1A, proteasome beta-subunit	PRINTS	PR00141	PROTEASOME	coord: 195..206 score: 51.89 coord: 63..78 score: 58.24 coord: 184..195 score: 71.78 coord: 220..231 score: 53.03
IPR029055	Nucleophile aminohydrolases, N-terminal	GENE3D	3.60.20.10	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	coord: 56..249 e-value: 5.6E-69 score: 234.2
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 44..230
IPR001353	Proteasome, subunit alpha/beta	PFAM	PF00227	Proteasome	coord: 54..228 e-value: 1.2E-47 score: 161.9
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 262..304
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 29..231
None	No IPR available	PANTHER	PTHR11599:SF154	PROTEASOME SUBUNIT BETA	coord: 262..304 coord: 29..231
None	No IPR available	CDD	cd03761	proteasome_beta_type_5	coord: 56..276 e-value: 1.3521E-126 score: 356.94
IPR016050	Proteasome beta-type subunit, conserved site	PROSITE	PS00854	PROTEASOME_BETA_1	coord: 59..106
IPR023333	Proteasome B-type subunit	PROSITE	PS51476	PROTEASOME_BETA_2	coord: 55..234 score: 53.822407

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000973.1	MS000973.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005839	proteasome core complex
molecular_function	GO:0004298	threonine-type endopeptidase activity