MS000956 (gene) Bitter gourd (TR) v1

Overview
NameMS000956
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRestriction endonuclease type II-like protein
Locationscaffold36: 458594 .. 460627 (-)
RNA-Seq ExpressionMS000956
SyntenyMS000956
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCGCTGCTGTCTCTTTCTCTCAATCTGGAGCATCCCGAGGTTTTCTTCACGGAGGTCCCTCTTTCAATCGTTTGCCGCGCGTCGCTTCATTATCAGCTCCCCGAGTTGATGCGTTCCGCTCAACTTCTCTTTTGGGTATTTAATCAAACACCTTATCTCTACTCGCTCCATGCCATTATAAGTATTTTGGGTATTCTCGATTTGGGGCTTTGCTTCCCGTTTTAAAACTACATGCCTTTGGCTAATTCTTTGCTTATTTTCGGAGATAGGAAACAAGTTATTCTGCTTGACATTGTTACTTGAAGGGTTCTTGTGATGAGCATTTTCCTTCTTATCTCCTGTTTTTTAAACATAATCTTGCTATCAACAATTTCTTCCTTATGAATTTATGTCGGACTCTAGAACCTTTCTCATGTCCTAACCATGTTTTAAAAACAACAAATTGGTTTCAATGGTTACAAGAAAGTAGAAAATAGAACAAAAAGGAAAATACCAAGACTAGAGTTTACTTTTTGCCAAGAGAAAAGAGAGAACTGGCTACAGAGGCGATTTATTCCTCTAATTTATGTGGGTTTTCAACTTACAACTGTGTTGTGTCGGTTGAATAAGAAATTCTACCTTAGTTTTGCTTTTAGGAATGCATCCAACATTATCTGCATTTGGGACATTAATCACTCTATGTTACTGTGAGGTCCATTTGTGCAATTCTTTGCATTATACTTTGGAGATTTGTGTTAAGGTCATTTCCAGGTTCATGATCATGTAAAATGGTGTACAAGATTCCTTTCCCTGTTTTAGATTGTTTCCTTGCACACCAAGTCACTTTTCACTGAAAAAAGAGGTAGAAAATAGAAATTAACTATTTTATTGGATAGAACTTAGAAGGTAACAAGCTCATCAACACCGTTGCTATAACAATTGTTTGTATTATCTTGAGTGCTTATAACCAGCAGAAACTGTCTAGGCTTATTTTTGTCGCTTTTCACTTCATTTACACATATAACATGCTAGCATTTTTATCTCAACTTCTAGCAGTCTGTGGGTTTTGCAGGACGCTTCATCAACGAAACTCTTCAATCAATACTACGATGTCAACGATGAGCAAAACCTCCATTTCTAGAATCTGCTGTAGACACCCTGGACTGAATGCGAGACTCTCCTCAAAACGAAAGCATGGGAGTGGTTCAAGAACTTTTTCAACATGCACCTCATCGTCGAGCTCCACAACGAACCCTCTGGTCATCCGTTTCCCCTCAGCTTTGATTTTGGCTTCCCAGGTCACCCCATCTGATGCACCTCAGCGTTCAGAAGAATGGTTTGCACTACGGAGAGACAGGTTGACTACAAGCACCTTCAGCACAGCCTTAGGCTTCTGGAAAGGAAACCGTCGCTTCGAGCTATGGCATGAGAAAGTGTTTCCTTATGAGATTCAAAAAACGGAAGCATCGCAACGGTGTGCCATGGAGTGGGGTGTGCTCAATGAAGCAACTGCAATTGATCGGTACAAAAGCATCACAGGCCGAGACGTAAGCTTGTTAGGGTTTGCAACTCACTCAGAACAGCAATTCGATTGGCTCGGCGCCTCCCCCGATGGCCTATTGGGATGCTTTCAAGGAGGAGGGATCCTGGAAGTAAAATGTCCATACAACAAGGGAAGGCCAGAGAAGGGACTACCCTGGCCAACAATGCCATTCTACTACATGCCACAGGTTCAGGGTCAAATGGAGATAATGGACAGAGAGTGGGTGGATCTATATTGTTGGACACCAAATGGAAGCACAATATTTCGTGTGTGTCGGGAGCGGGGTTATTGGGAACTGATGCGTGAAATGTTAAGGGAATTTTGGTGGGAAAACGTCGTTCCTGCGAGAGAGGCTCTATCTTTGGGAAGAGAGGAAGAGGTAAAGTCATATAAGCCAACATCCACGCATAAGAAGACTGGACTAGCAATTGCCAAGAGCATCAAATTAGCAAGCGAGGCCAAGTTGATGTGTAGGGAGATAGCTGGACATGTTGAATTTTACCGA

mRNA sequence

ATGAAGCTCGCTGCTGTCTCTTTCTCTCAATCTGGAGCATCCCGAGGTTTTCTTCACGGAGGTCCCTCTTTCAATCGTTTGCCGCGCGTCGCTTCATTATCAGCTCCCCGAGTTGATGCGTTCCGCTCAACTTCTCTTTTGGCAGTCTGTGGGTTTTGCAGGACGCTTCATCAACGAAACTCTTCAATCAATACTACGATGTCAACGATGAGCAAAACCTCCATTTCTAGAATCTGCTGTAGACACCCTGGACTGAATGCGAGACTCTCCTCAAAACGAAAGCATGGGAGTGGTTCAAGAACTTTTTCAACATGCACCTCATCGTCGAGCTCCACAACGAACCCTCTGGTCATCCGTTTCCCCTCAGCTTTGATTTTGGCTTCCCAGGTCACCCCATCTGATGCACCTCAGCGTTCAGAAGAATGGTTTGCACTACGGAGAGACAGGTTGACTACAAGCACCTTCAGCACAGCCTTAGGCTTCTGGAAAGGAAACCGTCGCTTCGAGCTATGGCATGAGAAAGTGTTTCCTTATGAGATTCAAAAAACGGAAGCATCGCAACGGTGTGCCATGGAGTGGGGTGTGCTCAATGAAGCAACTGCAATTGATCGGTACAAAAGCATCACAGGCCGAGACGTAAGCTTGTTAGGGTTTGCAACTCACTCAGAACAGCAATTCGATTGGCTCGGCGCCTCCCCCGATGGCCTATTGGGATGCTTTCAAGGAGGAGGGATCCTGGAAGTAAAATGTCCATACAACAAGGGAAGGCCAGAGAAGGGACTACCCTGGCCAACAATGCCATTCTACTACATGCCACAGGTTCAGGGTCAAATGGAGATAATGGACAGAGAGTGGGTGGATCTATATTGTTGGACACCAAATGGAAGCACAATATTTCGTGTGTGTCGGGAGCGGGGTTATTGGGAACTGATGCGTGAAATGTTAAGGGAATTTTGGTGGGAAAACGTCGTTCCTGCGAGAGAGGCTCTATCTTTGGGAAGAGAGGAAGAGGTAAAGTCATATAAGCCAACATCCACGCATAAGAAGACTGGACTAGCAATTGCCAAGAGCATCAAATTAGCAAGCGAGGCCAAGTTGATGTGTAGGGAGATAGCTGGACATGTTGAATTTTACCGA

Coding sequence (CDS)

ATGAAGCTCGCTGCTGTCTCTTTCTCTCAATCTGGAGCATCCCGAGGTTTTCTTCACGGAGGTCCCTCTTTCAATCGTTTGCCGCGCGTCGCTTCATTATCAGCTCCCCGAGTTGATGCGTTCCGCTCAACTTCTCTTTTGGCAGTCTGTGGGTTTTGCAGGACGCTTCATCAACGAAACTCTTCAATCAATACTACGATGTCAACGATGAGCAAAACCTCCATTTCTAGAATCTGCTGTAGACACCCTGGACTGAATGCGAGACTCTCCTCAAAACGAAAGCATGGGAGTGGTTCAAGAACTTTTTCAACATGCACCTCATCGTCGAGCTCCACAACGAACCCTCTGGTCATCCGTTTCCCCTCAGCTTTGATTTTGGCTTCCCAGGTCACCCCATCTGATGCACCTCAGCGTTCAGAAGAATGGTTTGCACTACGGAGAGACAGGTTGACTACAAGCACCTTCAGCACAGCCTTAGGCTTCTGGAAAGGAAACCGTCGCTTCGAGCTATGGCATGAGAAAGTGTTTCCTTATGAGATTCAAAAAACGGAAGCATCGCAACGGTGTGCCATGGAGTGGGGTGTGCTCAATGAAGCAACTGCAATTGATCGGTACAAAAGCATCACAGGCCGAGACGTAAGCTTGTTAGGGTTTGCAACTCACTCAGAACAGCAATTCGATTGGCTCGGCGCCTCCCCCGATGGCCTATTGGGATGCTTTCAAGGAGGAGGGATCCTGGAAGTAAAATGTCCATACAACAAGGGAAGGCCAGAGAAGGGACTACCCTGGCCAACAATGCCATTCTACTACATGCCACAGGTTCAGGGTCAAATGGAGATAATGGACAGAGAGTGGGTGGATCTATATTGTTGGACACCAAATGGAAGCACAATATTTCGTGTGTGTCGGGAGCGGGGTTATTGGGAACTGATGCGTGAAATGTTAAGGGAATTTTGGTGGGAAAACGTCGTTCCTGCGAGAGAGGCTCTATCTTTGGGAAGAGAGGAAGAGGTAAAGTCATATAAGCCAACATCCACGCATAAGAAGACTGGACTAGCAATTGCCAAGAGCATCAAATTAGCAAGCGAGGCCAAGTTGATGTGTAGGGAGATAGCTGGACATGTTGAATTTTACCGA

Protein sequence

MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRNSSINTTMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIRFPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEIQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFRVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIKLASEAKLMCREIAGHVEFYR
Homology
BLAST of MS000956 vs. NCBI nr
Match: XP_022131520.1 (uncharacterized protein LOC111004694 isoform X1 [Momordica charantia])

HSP 1 Score: 760.4 bits (1962), Expect = 7.5e-216
Identity = 374/379 (98.68%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLL VCGFCRTLHQRN
Sbjct: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLL-VCGFCRTLHQRN 60

Query: 61  SSINTTMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIRF 120
           SSINTTMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLV RF
Sbjct: 61  SSINTTMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVTRF 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI 180
           PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI
Sbjct: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI 180

Query: 181 QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF
Sbjct: 181 QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGRPEKGLPW TMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGRPEKGLPWSTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR 300

Query: 301 VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIKL 360
           VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHK+TGLAIAKSIKL
Sbjct: 301 VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKQTGLAIAKSIKL 360

Query: 361 ASEAKLMCREIAGHVEFYR 380
           ASEAKLM REIAGHVEFYR
Sbjct: 361 ASEAKLMFREIAGHVEFYR 378

BLAST of MS000956 vs. NCBI nr
Match: XP_038884042.1 (uncharacterized protein LOC120074988 isoform X1 [Benincasa hispida])

HSP 1 Score: 672.2 bits (1733), Expect = 2.7e-189
Identity = 332/380 (87.37%), Postives = 349/380 (91.84%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFS++GAS+  LHGG SFNR PRVAS +A +VDAF STSLL VCG CRTLH  N
Sbjct: 1   MKLAAVSFSRAGASQRLLHGGSSFNRFPRVASFAARQVDAFSSTSLL-VCGLCRTLHHSN 60

Query: 61  SSINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIR 120
           SS+ T  MSTM+ TSISRICCRH  +NARL  +RKHGSGSRTFSTC S SSS TNPLVIR
Sbjct: 61  SSVETAIMSTMNNTSISRICCRHSRMNARL-LRRKHGSGSRTFSTCASPSSSITNPLVIR 120

Query: 121 FPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYE 180
            PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFP E
Sbjct: 121 LPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPPE 180

Query: 181 IQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240
           IQK EA Q+ AMEWGVLNEA AIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC
Sbjct: 181 IQKPEAPQQNAMEWGVLNEANAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240

Query: 241 FQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIF 300
           FQGGGILEVKCPYNKG+PEKGLPW TMPFYYMPQVQGQMEIMDREW DLYCWTPNGSTIF
Sbjct: 241 FQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQMEIMDREWADLYCWTPNGSTIF 300

Query: 301 RVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIK 360
           RVCRERGYW+L+REMLREFWWENVVPAREAL LGREE+VKSYKPTSTHK+TGLAIAKSIK
Sbjct: 301 RVCRERGYWDLIREMLREFWWENVVPAREALLLGREEQVKSYKPTSTHKQTGLAIAKSIK 360

Query: 361 LASEAKLMCREIAGHVEFYR 380
           LASEAKL+CREIAGH+EFYR
Sbjct: 361 LASEAKLLCREIAGHIEFYR 378

BLAST of MS000956 vs. NCBI nr
Match: XP_023537251.1 (uncharacterized protein LOC111798383 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649.0 bits (1673), Expect = 2.4e-182
Identity = 321/380 (84.47%), Postives = 340/380 (89.47%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFSQSGASR FLH   SFNRLPRVAS SA +VDAF STS L+VCGFCRT HQ N
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPRVASFSARQVDAFSSTS-LSVCGFCRTPHQSN 60

Query: 61  SSINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIR 120
           SSI+T  +STMS TSI+RICCRHP  NARL SKRK  +GSRTFST  S   S TNPL+IR
Sbjct: 61  SSIDTALLSTMSNTSIARICCRHPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIR 120

Query: 121 FPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYE 180
            PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP E
Sbjct: 121 LPSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSE 180

Query: 181 IQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240
           I+K EA Q+ AMEWGVLNE  AI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGC
Sbjct: 181 IKKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGC 240

Query: 241 FQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIF 300
           FQGGGILEVKCPYNKG+PEKGLPW TMPFYYMPQVQGQ+EIMDREW DLYCWTPNGSTIF
Sbjct: 241 FQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIF 300

Query: 301 RVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIK 360
           RVCRERGYWEL+ EMLREFWWENVVPAREA S GRE+EV+SYKPTSTHK TG+AIAKSIK
Sbjct: 301 RVCRERGYWELIHEMLREFWWENVVPAREASSSGREKEVESYKPTSTHKLTGVAIAKSIK 360

Query: 361 LASEAKLMCREIAGHVEFYR 380
           LAS+AKL+CREIAGHVEFYR
Sbjct: 361 LASDAKLLCREIAGHVEFYR 379

BLAST of MS000956 vs. NCBI nr
Match: KAG7020121.1 (hypothetical protein SDJN02_16803 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 647.1 bits (1668), Expect = 9.3e-182
Identity = 321/380 (84.47%), Postives = 340/380 (89.47%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFSQSGASR FLH   SFNRLP VAS SA +VDAF STS L+VCGFCRT HQ N
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPCVASFSARQVDAFSSTS-LSVCGFCRTPHQSN 60

Query: 61  SSINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIR 120
           SSINT  +STMS TSI+RICCR P  NARL SKRK  +GSRTFST  S   S TNPL+IR
Sbjct: 61  SSINTALLSTMSNTSIARICCRLPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIR 120

Query: 121 FPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYE 180
            PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP E
Sbjct: 121 LPSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSE 180

Query: 181 IQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240
           I+K EA Q+ AMEWGVLNE  AI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGC
Sbjct: 181 IKKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGC 240

Query: 241 FQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIF 300
           FQGGGILEVKCPYNKG+PEKGLPW TMPFYYMPQVQGQ+EIMDREW DLYCWTPNGSTIF
Sbjct: 241 FQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIF 300

Query: 301 RVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIK 360
           RVCRERGYWEL+ EMLREFWWENVVPAREALS GRE+EV+SYKPTSTHK+TG+AIAKSIK
Sbjct: 301 RVCRERGYWELIHEMLREFWWENVVPAREALSSGREKEVESYKPTSTHKQTGVAIAKSIK 360

Query: 361 LASEAKLMCREIAGHVEFYR 380
           LAS+AKL+CREIAGHVEFYR
Sbjct: 361 LASDAKLLCREIAGHVEFYR 379

BLAST of MS000956 vs. NCBI nr
Match: XP_022951164.1 (uncharacterized protein LOC111454092 isoform X1 [Cucurbita moschata])

HSP 1 Score: 644.8 bits (1662), Expect = 4.6e-181
Identity = 320/380 (84.21%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFSQSGASR FLH   SFNRLP VAS SA +VDAF STS  +VCGFCRT HQ N
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPCVASFSARQVDAFSSTS-RSVCGFCRTPHQSN 60

Query: 61  SSINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIR 120
           SSINT  +STMS TSI+RICCR P  NARL SKRK  +GSRTFST  S   S TNPL+IR
Sbjct: 61  SSINTALLSTMSNTSIARICCRLPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIR 120

Query: 121 FPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYE 180
            PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP E
Sbjct: 121 LPSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSE 180

Query: 181 IQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240
           I+K EA Q+ AMEWGVLNE  AI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGC
Sbjct: 181 IKKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGC 240

Query: 241 FQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIF 300
           FQGGGILEVKCPYNKG+PEKGLPW TMPFYYMPQVQGQ+EIMDREW DLYCWTPNGSTIF
Sbjct: 241 FQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIF 300

Query: 301 RVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIK 360
           RVCRERGYWEL+ EMLREFWWENVVPAREALS GRE+EV+SYKPTSTHK+TG+AIAKSIK
Sbjct: 301 RVCRERGYWELIHEMLREFWWENVVPAREALSSGREKEVESYKPTSTHKQTGVAIAKSIK 360

Query: 361 LASEAKLMCREIAGHVEFYR 380
           LAS+AKL+CREIAGHVEFYR
Sbjct: 361 LASDAKLLCREIAGHVEFYR 379

BLAST of MS000956 vs. ExPASy TrEMBL
Match: A0A6J1BPX6 (uncharacterized protein LOC111004694 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004694 PE=4 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 3.6e-216
Identity = 374/379 (98.68%), Postives = 375/379 (98.94%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLL VCGFCRTLHQRN
Sbjct: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLL-VCGFCRTLHQRN 60

Query: 61  SSINTTMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIRF 120
           SSINTTMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLV RF
Sbjct: 61  SSINTTMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVTRF 120

Query: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI 180
           PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI
Sbjct: 121 PSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEI 180

Query: 181 QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240
           QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF
Sbjct: 181 QKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCF 240

Query: 241 QGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR 300
           QGGGILEVKCPYNKGRPEKGLPW TMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR
Sbjct: 241 QGGGILEVKCPYNKGRPEKGLPWSTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFR 300

Query: 301 VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIKL 360
           VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHK+TGLAIAKSIKL
Sbjct: 301 VCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKQTGLAIAKSIKL 360

Query: 361 ASEAKLMCREIAGHVEFYR 380
           ASEAKLM REIAGHVEFYR
Sbjct: 361 ASEAKLMFREIAGHVEFYR 378

BLAST of MS000956 vs. ExPASy TrEMBL
Match: A0A6J1GHX8 (uncharacterized protein LOC111454092 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454092 PE=4 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.2e-181
Identity = 320/380 (84.21%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFSQSGASR FLH   SFNRLP VAS SA +VDAF STS  +VCGFCRT HQ N
Sbjct: 1   MKLAAVSFSQSGASRSFLHADSSFNRLPCVASFSARQVDAFSSTS-RSVCGFCRTPHQSN 60

Query: 61  SSINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIR 120
           SSINT  +STMS TSI+RICCR P  NARL SKRK  +GSRTFST  S   S TNPL+IR
Sbjct: 61  SSINTALLSTMSNTSIARICCRLPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIR 120

Query: 121 FPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYE 180
            PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKGNRRFELWHEKVFP E
Sbjct: 121 LPSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRFELWHEKVFPSE 180

Query: 181 IQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240
           I+K EA Q+ AMEWGVLNE  AI RYKSITGRDVS LGFATHSEQQ DWLGASPDGLLGC
Sbjct: 181 IKKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLDWLGASPDGLLGC 240

Query: 241 FQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIF 300
           FQGGGILEVKCPYNKG+PEKGLPW TMPFYYMPQVQGQ+EIMDREW DLYCWTPNGSTIF
Sbjct: 241 FQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIF 300

Query: 301 RVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIK 360
           RVCRERGYWEL+ EMLREFWWENVVPAREALS GRE+EV+SYKPTSTHK+TG+AIAKSIK
Sbjct: 301 RVCRERGYWELIHEMLREFWWENVVPAREALSSGREKEVESYKPTSTHKQTGVAIAKSIK 360

Query: 361 LASEAKLMCREIAGHVEFYR 380
           LAS+AKL+CREIAGHVEFYR
Sbjct: 361 LASDAKLLCREIAGHVEFYR 379

BLAST of MS000956 vs. ExPASy TrEMBL
Match: A0A6J1KS64 (uncharacterized protein LOC111496007 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496007 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.9e-181
Identity = 318/380 (83.68%), Postives = 340/380 (89.47%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MKLAAVSFSQS ASR FLH   SFNRLPRVAS S P+VDAF STS L+VC FCRT HQ N
Sbjct: 1   MKLAAVSFSQSVASRSFLHADSSFNRLPRVASFSPPQVDAFSSTS-LSVCWFCRTPHQSN 60

Query: 61  SSINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIR 120
           SSI+T  +STMS TSI+RICC HP  NARL SKRK  +GSRTFST  S   S TNPL+IR
Sbjct: 61  SSIDTALLSTMSNTSIARICCTHPRSNARLFSKRKQWNGSRTFSTSISPPKSVTNPLLIR 120

Query: 121 FPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYE 180
            PSALI+ASQVTPSDAPQRSEEWFALRRD+LTTSTFSTALGFWKG+RRFELWHEKVFP E
Sbjct: 121 LPSALIVASQVTPSDAPQRSEEWFALRRDKLTTSTFSTALGFWKGSRRFELWHEKVFPSE 180

Query: 181 IQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240
           I+K EA Q+ AMEWGVLNE  AI RYKSITGRDVS LGFATHSEQQ +WLGASPDGLLGC
Sbjct: 181 IKKPEARQQYAMEWGVLNEENAIHRYKSITGRDVSFLGFATHSEQQLEWLGASPDGLLGC 240

Query: 241 FQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIF 300
           FQGGGILEVKCPYNKG+PEKGLPW TMPFYYMPQVQGQ+EIMDREW DLYCWTPNGSTIF
Sbjct: 241 FQGGGILEVKCPYNKGKPEKGLPWSTMPFYYMPQVQGQLEIMDREWADLYCWTPNGSTIF 300

Query: 301 RVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIK 360
           RVCRERGYWEL+ EMLREFWWENVVPAREALSLGREEEV+SYKPTSTHK+TG+AI+KSIK
Sbjct: 301 RVCRERGYWELIHEMLREFWWENVVPAREALSLGREEEVESYKPTSTHKQTGVAISKSIK 360

Query: 361 LASEAKLMCREIAGHVEFYR 380
           LAS+AKL+CREIAGHVEFYR
Sbjct: 361 LASDAKLLCREIAGHVEFYR 379

BLAST of MS000956 vs. ExPASy TrEMBL
Match: A0A6J1BPQ6 (uncharacterized protein LOC111004694 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004694 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 9.4e-180
Identity = 309/313 (98.72%), Postives = 310/313 (99.04%), Query Frame = 0

Query: 67  MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIRFPSALIL 126
           MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLV RFPSALIL
Sbjct: 1   MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVTRFPSALIL 60

Query: 127 ASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEIQKTEAS 186
           ASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEIQKTEAS
Sbjct: 61  ASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEIQKTEAS 120

Query: 187 QRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQGGGIL 246
           QRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQGGGIL
Sbjct: 121 QRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQGGGIL 180

Query: 247 EVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFRVCRERG 306
           EVKCPYNKGRPEKGLPW TMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFRVCRERG
Sbjct: 181 EVKCPYNKGRPEKGLPWSTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFRVCRERG 240

Query: 307 YWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIKLASEAKL 366
           YWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHK+TGLAIAKSIKLASEAKL
Sbjct: 241 YWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKQTGLAIAKSIKLASEAKL 300

Query: 367 MCREIAGHVEFYR 380
           M REIAGHVEFYR
Sbjct: 301 MFREIAGHVEFYR 313

BLAST of MS000956 vs. ExPASy TrEMBL
Match: A0A0A0LNG4 (YqaJ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G350400 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.6e-171
Identity = 305/380 (80.26%), Postives = 324/380 (85.26%), Query Frame = 0

Query: 1   MKLAAVSFSQSGASRGFLHGGPSFNRLPRVASLSAPRVDAFRSTSLLAVCGFCRTLHQRN 60
           MK AAVSFSQSGASR  LHGG SFN+L  VAS+SA +  +F S SLL VCG CRTL Q +
Sbjct: 1   MKFAAVSFSQSGASRSLLHGGSSFNQLLPVASISARQFGSFNSNSLL-VCGLCRTLRQSS 60

Query: 61  SSINTT-MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIR 120
           S + T  MSTM+  SI+RICCRH   NARL  KR H   SR FSTC S SSST NPLVI 
Sbjct: 61  SLVETAIMSTMNNISIARICCRHSRKNARLYLKRNHEIASRPFSTCVSPSSSTKNPLVIW 120

Query: 121 FPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYE 180
            PS L+LASQ   S APQRSEEWFALRRD+LTTSTFSTALGFWKGNRR ELWHEKVFP E
Sbjct: 121 LPSPLVLASQANQSVAPQRSEEWFALRRDKLTTSTFSTALGFWKGNRRIELWHEKVFPSE 180

Query: 181 IQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGC 240
           IQKTEA Q+ AMEWGVLNE  AIDRYK ITGRDVSLLGFATHSEQQFDWLGASPDGLL C
Sbjct: 181 IQKTEAPQQNAMEWGVLNEVNAIDRYKGITGRDVSLLGFATHSEQQFDWLGASPDGLLEC 240

Query: 241 FQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIF 300
           FQGGGILEVKCPYNKG+PEKGLPW T+PFYYMPQVQGQMEIM REW DLYCWTPNGSTIF
Sbjct: 241 FQGGGILEVKCPYNKGKPEKGLPWSTIPFYYMPQVQGQMEIMGREWADLYCWTPNGSTIF 300

Query: 301 RVCRERGYWELMREMLREFWWENVVPAREALSLGREEEVKSYKPTSTHKKTGLAIAKSIK 360
           RVCRERGYW+L+RE+LREFWWENVVPA+EAL LG EE+ KSYKPTSTHK+TGLAIAKSIK
Sbjct: 301 RVCRERGYWDLIREILREFWWENVVPAKEALLLGSEEKAKSYKPTSTHKQTGLAIAKSIK 360

Query: 361 LASEAKLMCREIAGHVEFYR 380
           LASEAKL CREIAGHVEFYR
Sbjct: 361 LASEAKLFCREIAGHVEFYR 379

BLAST of MS000956 vs. TAIR 10
Match: AT1G67660.3 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 386.0 bits (990), Expect = 3.6e-107
Identity = 194/336 (57.74%), Postives = 248/336 (73.81%), Query Frame = 0

Query: 45  SLLAVCGFCRTLHQRNSSINT-TMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFS 104
           + + VC  CR L     ++N+  +S M   SIS      P  +  +SS+++    S   S
Sbjct: 10  TFVTVC-VCRVLKPNKVALNSMILSAMRTCSISGFHTHLPKSSGSVSSRKRF--SSTALS 69

Query: 105 TCTSSSSSTTNPLVIRFPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWK 164
             T + S   +P      S++I++S ++PSD PQ+SEEWFALR+D+LTTSTFSTALGFWK
Sbjct: 70  LITQTISPFAHP-----RSSVIVSSLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWK 129

Query: 165 GNRRFELWHEKVFPYEIQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSE 224
           GNRR ELWHEKV+  + +  E S R AM WGV  E++AI+RYK I G +V  +GFA HS 
Sbjct: 130 GNRRAELWHEKVYDSDARVVEESARFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIHSN 189

Query: 225 QQFDWLGASPDGLLGCFQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDR 284
           ++F WLGASPDG+L CF   GILEVKCPYNKG+ E  LPW  +P+YYMPQ+QGQMEIMDR
Sbjct: 190 EEFHWLGASPDGILDCF---GILEVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDR 249

Query: 285 EWVDLYCWTPNGSTIFRVCRERGYWELMREMLREFWWENVVPAREALSLGRE-EEVKSYK 344
           EWV+LYCWT NGST+FRV R+R YW ++ ++LREFWWE+V+PAREAL LG+E EEVK Y+
Sbjct: 250 EWVNLYCWTRNGSTVFRVMRDRSYWRIIHDVLREFWWESVIPAREALLLGKEDEEVKKYE 309

Query: 345 PTSTHKKTGLAIAKSIKLASEAKLMCREIAGHVEFY 379
           PTSTHK+T LAIAKS+ LA+E+KL+CREIA HVEF+
Sbjct: 310 PTSTHKRTKLAIAKSLNLAAESKLVCREIADHVEFF 334

BLAST of MS000956 vs. TAIR 10
Match: AT1G67660.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.7e-107
Identity = 194/333 (58.26%), Postives = 247/333 (74.17%), Query Frame = 0

Query: 48  AVCGFCRTLHQRNSSINT-TMSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCT 107
           +VC  CR L     ++N+  +S M   SIS      P  +  +SS+++    S   S  T
Sbjct: 34  SVC-VCRVLKPNKVALNSMILSAMRTCSISGFHTHLPKSSGSVSSRKRF--SSTALSLIT 93

Query: 108 SSSSSTTNPLVIRFPSALILASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNR 167
            + S   +P      S++I++S ++PSD PQ+SEEWFALR+D+LTTSTFSTALGFWKGNR
Sbjct: 94  QTISPFAHP-----RSSVIVSSLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKGNR 153

Query: 168 RFELWHEKVFPYEIQKTEASQRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQF 227
           R ELWHEKV+  + +  E S R AM WGV  E++AI+RYK I G +V  +GFA HS ++F
Sbjct: 154 RAELWHEKVYDSDARVVEESARFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIHSNEEF 213

Query: 228 DWLGASPDGLLGCFQGGGILEVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWV 287
            WLGASPDG+L CF   GILEVKCPYNKG+ E  LPW  +P+YYMPQ+QGQMEIMDREWV
Sbjct: 214 HWLGASPDGILDCF---GILEVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDREWV 273

Query: 288 DLYCWTPNGSTIFRVCRERGYWELMREMLREFWWENVVPAREALSLGRE-EEVKSYKPTS 347
           +LYCWT NGST+FRV R+R YW ++ ++LREFWWE+V+PAREAL LG+E EEVK Y+PTS
Sbjct: 274 NLYCWTRNGSTVFRVMRDRSYWRIIHDVLREFWWESVIPAREALLLGKEDEEVKKYEPTS 333

Query: 348 THKKTGLAIAKSIKLASEAKLMCREIAGHVEFY 379
           THK+T LAIAKS+ LA+E+KL+CREIA HVEF+
Sbjct: 334 THKRTKLAIAKSLNLAAESKLVCREIADHVEFF 355

BLAST of MS000956 vs. TAIR 10
Match: AT1G67660.2 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 4.0e-106
Identity = 188/313 (60.06%), Postives = 237/313 (75.72%), Query Frame = 0

Query: 67  MSTMSKTSISRICCRHPGLNARLSSKRKHGSGSRTFSTCTSSSSSTTNPLVIRFPSALIL 126
           +S M   SIS      P  +  +SS+++    S   S  T + S   +P      S++I+
Sbjct: 3   LSAMRTCSISGFHTHLPKSSGSVSSRKRF--SSTALSLITQTISPFAHP-----RSSVIV 62

Query: 127 ASQVTPSDAPQRSEEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEIQKTEAS 186
           +S ++PSD PQ+SEEWFALR+D+LTTSTFSTALGFWKGNRR ELWHEKV+  + +  E S
Sbjct: 63  SSLLSPSDIPQKSEEWFALRKDKLTTSTFSTALGFWKGNRRAELWHEKVYDSDARVVEES 122

Query: 187 QRCAMEWGVLNEATAIDRYKSITGRDVSLLGFATHSEQQFDWLGASPDGLLGCFQGGGIL 246
            R AM WGV  E++AI+RYK I G +V  +GFA HS ++F WLGASPDG+L CF   GIL
Sbjct: 123 ARFAMNWGVQMESSAIERYKRIMGCEVGTMGFAIHSNEEFHWLGASPDGILDCF---GIL 182

Query: 247 EVKCPYNKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFRVCRERG 306
           EVKCPYNKG+ E  LPW  +P+YYMPQ+QGQMEIMDREWV+LYCWT NGST+FRV R+R 
Sbjct: 183 EVKCPYNKGKTETVLPWKKVPYYYMPQLQGQMEIMDREWVNLYCWTRNGSTVFRVMRDRS 242

Query: 307 YWELMREMLREFWWENVVPAREALSLGRE-EEVKSYKPTSTHKKTGLAIAKSIKLASEAK 366
           YW ++ ++LREFWWE+V+PAREAL LG+E EEVK Y+PTSTHK+T LAIAKS+ LA+E+K
Sbjct: 243 YWRIIHDVLREFWWESVIPAREALLLGKEDEEVKKYEPTSTHKRTKLAIAKSLNLAAESK 302

Query: 367 LMCREIAGHVEFY 379
           L+CREIA HVEF+
Sbjct: 303 LVCREIADHVEFF 305

BLAST of MS000956 vs. TAIR 10
Match: AT1G13810.1 (Restriction endonuclease, type II-like superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.8e-42
Identity = 90/247 (36.44%), Postives = 143/247 (57.89%), Query Frame = 0

Query: 140 EEWFALRRDRLTTSTFSTALGFWKGNRRFELWHEKVFPYEIQKTEASQRCAMEWGVLNEA 199
           + W  LR++RLT S F+ A+GF    RR  LW EK+      K  A  R A  W + NE 
Sbjct: 60  KNWEDLRKNRLTASNFARAIGFSPDGRR-NLWLEKI---GAAKPFAGNR-ATFWDIENEV 119

Query: 200 TAIDRYKSITGRDVSLLGFATH---SEQQFDWLGASPDGLLGCFQGG----GILEVKCPY 259
            A++RY  +TG ++ +  F  +      + +WLGASPDG++   + G    G+LEVKCP+
Sbjct: 120 EALERYNELTGNEILIPEFVVYKNGESPEENWLGASPDGVINVVKDGVTSCGVLEVKCPF 179

Query: 260 NKGRPEKGLPWPTMPFYYMPQVQGQMEIMDREWVDLYCWTPNGSTIFRVCRERGYWELMR 319
           +     K  PW  +P+  +PQ+QG MEI+D +W+DLYCWT NGS++FRV R+  +WE M+
Sbjct: 180 DNRDNSKVYPWKKVPYNCVPQLQGLMEIVDTDWLDLYCWTRNGSSLFRVWRDTAFWEDMK 239

Query: 320 EMLREFWWENVVPAREALS----LGREEEVKSYKPTSTHKKTGLAIAKSIKLASEAKLMC 376
             L +FW  +V+PARE  +       + +++ +KP   H+     +  + ++++ A  + 
Sbjct: 240 PALFDFWQNHVLPAREIYNNFDIKDPQVKLREFKPKHWHEDCKKIMRGAERISANANRLF 299

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131520.17.5e-21698.68uncharacterized protein LOC111004694 isoform X1 [Momordica charantia][more]
XP_038884042.12.7e-18987.37uncharacterized protein LOC120074988 isoform X1 [Benincasa hispida][more]
XP_023537251.12.4e-18284.47uncharacterized protein LOC111798383 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7020121.19.3e-18284.47hypothetical protein SDJN02_16803 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022951164.14.6e-18184.21uncharacterized protein LOC111454092 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1BPX63.6e-21698.68uncharacterized protein LOC111004694 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1GHX82.2e-18184.21uncharacterized protein LOC111454092 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KS642.9e-18183.68uncharacterized protein LOC111496007 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1BPQ69.4e-18098.72uncharacterized protein LOC111004694 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A0A0LNG41.6e-17180.26YqaJ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G350400 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT1G67660.33.6e-10757.74Restriction endonuclease, type II-like superfamily protein [more]
AT1G67660.14.7e-10758.26Restriction endonuclease, type II-like superfamily protein [more]
AT1G67660.24.0e-10660.06Restriction endonuclease, type II-like superfamily protein [more]
AT1G13810.11.8e-4236.44Restriction endonuclease, type II-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019080YqaJ viral recombinasePFAMPF09588YqaJcoord: 141..283
e-value: 5.9E-20
score: 72.0
IPR017482Putative phage-type endonucleaseTIGRFAMTIGR03033TIGR03033coord: 136..292
e-value: 1.7E-20
score: 71.7
IPR011604Exonuclease, phage-type/RecB, C-terminalGENE3D3.90.320.10coord: 122..327
e-value: 4.5E-49
score: 168.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 96..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..110
NoneNo IPR availablePANTHERPTHR46609:SF6RESTRICTION ENDONUCLEASE, TYPE II-LIKE SUPERFAMILY PROTEINcoord: 67..378
NoneNo IPR availablePANTHERPTHR46609EXONUCLEASE, PHAGE-TYPE/RECB, C-TERMINAL DOMAIN-CONTAINING PROTEINcoord: 67..378
IPR011335Restriction endonuclease type II-likeSUPERFAMILY52980Restriction endonuclease-likecoord: 133..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000956.1MS000956.1mRNA