MS000950 (gene) Bitter gourd (TR) v1

Overview


Name	MS000950
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	HXXXD-type acyl-transferase family protein
Location	scaffold36: 420838 .. 421344 (+)
RNA-Seq Expression	MS000950
Synteny	MS000950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAGAAGTTGATGTAATCTCCGAAGTGTTGATAAAACCTTCTTGTCCAACTCCTCACCATCTCCGCCGCTACACCCTCTCCTTCATCGACCAGATCACCGTCCACATCTACGCCCCCGCCCTCTACTTCTACCAATCCAACGGCGATCCCGACTTCGCCGCCGCCTTCCAACGCCGCCTCCAGACCTCCCTCTCCGCCCTCCTCACCCACTACCCTCCCCTCGCCGGCCGCCCCAACTACGCCGCCTCCTTCGTCGACTGCAACGACGCCGGCGTCCCGTTCCGCGAGGCCGTGACCGGGTCCACGCTCTCCGACGTGGTCCACCACGCCACCCCGGACGACCTGAACCGGCTCTTCCCGGTCGAGCTGGACCGGTGCAACGATGAGCTGATGATGGTCCAGTTCACGGAATTGGCGTGCGGGGGAGTGGCCGTCGCGTGTTGCGTTTCGCACAAGATTGCGGACGCGATGACGCTGTTCTCGCTGAATAACAACTGGGCGGCGTTG

mRNA sequence

CTAGAAGTTGATGTAATCTCCGAAGTGTTGATAAAACCTTCTTGTCCAACTCCTCACCATCTCCGCCGCTACACCCTCTCCTTCATCGACCAGATCACCGTCCACATCTACGCCCCCGCCCTCTACTTCTACCAATCCAACGGCGATCCCGACTTCGCCGCCGCCTTCCAACGCCGCCTCCAGACCTCCCTCTCCGCCCTCCTCACCCACTACCCTCCCCTCGCCGGCCGCCCCAACTACGCCGCCTCCTTCGTCGACTGCAACGACGCCGGCGTCCCGTTCCGCGAGGCCGTGACCGGGTCCACGCTCTCCGACGTGGTCCACCACGCCACCCCGGACGACCTGAACCGGCTCTTCCCGGTCGAGCTGGACCGGTGCAACGATGAGCTGATGATGGTCCAGTTCACGGAATTGGCGTGCGGGGGAGTGGCCGTCGCGTGTTGCGTTTCGCACAAGATTGCGGACGCGATGACGCTGTTCTCGCTGAATAACAACTGGGCGGCGTTG

Coding sequence (CDS)

CTAGAAGTTGATGTAATCTCCGAAGTGTTGATAAAACCTTCTTGTCCAACTCCTCACCATCTCCGCCGCTACACCCTCTCCTTCATCGACCAGATCACCGTCCACATCTACGCCCCCGCCCTCTACTTCTACCAATCCAACGGCGATCCCGACTTCGCCGCCGCCTTCCAACGCCGCCTCCAGACCTCCCTCTCCGCCCTCCTCACCCACTACCCTCCCCTCGCCGGCCGCCCCAACTACGCCGCCTCCTTCGTCGACTGCAACGACGCCGGCGTCCCGTTCCGCGAGGCCGTGACCGGGTCCACGCTCTCCGACGTGGTCCACCACGCCACCCCGGACGACCTGAACCGGCTCTTCCCGGTCGAGCTGGACCGGTGCAACGATGAGCTGATGATGGTCCAGTTCACGGAATTGGCGTGCGGGGGAGTGGCCGTCGCGTGTTGCGTTTCGCACAAGATTGCGGACGCGATGACGCTGTTCTCGCTGAATAACAACTGGGCGGCGTTG

Protein sequence

LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQRRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRLFPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL

Homology

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BLAST of MS000950 vs. NCBI nr
Match: XP_004143048.1 (stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical protein Csa_018752 [Cucumis sativus])

HSP 1 Score: 270.0 bits (689), Expect = 1.4e-68
Identity = 131/175 (74.86%), Postives = 147/175 (84.00%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNG------DPDFAA 60
           LEVD+ISE LIKPS PTP HLR Y+LSFIDQITVHIYAPALYFYQS        D DFA 
Sbjct: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDFAL 62

Query: 61  AFQRRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDD 120
           AF+ RL+++L  +L+HYPPLAGRPNYA+SF+DCND GVPFREA+  S L+DV+  A PDD
Sbjct: 63  AFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQPDD 122

Query: 121 LNRLFPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           LNRLFPVELDR N+ELM VQFTE ACGGVAVA C+SHKIADAMTLFSLNNNWAA+
Sbjct: 123 LNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 177

[Close]

BLAST of MS000950 vs. NCBI nr
Match: KAG7020113.1 (Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 266.9 bits (681), Expect = 1.2e-67
Identity = 128/171 (74.85%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSN--GDPDFAAAFQR 60
           LE+D+ISE LIKP+ PTP HLRRY LSFIDQITVHIYAPALYFYQ N  GDPDFA  F+ 
Sbjct: 3   LEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDFRN 62

Query: 61  RLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRL 120
           RL+++LS +L+HYPPLAGRPNY ++FVDCND G  FREA   + L+DVV +A PDDLNRL
Sbjct: 63  RLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLNRL 122

Query: 121 FPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           FPVELD+ N+ELM VQFTE ACGGVAVA C+SHKIADAMTLFSLNNNWAA+
Sbjct: 123 FPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 173

[Close]

BLAST of MS000950 vs. NCBI nr
Match: KAG6585195.1 (Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 266.9 bits (681), Expect = 1.2e-67
Identity = 128/171 (74.85%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSN--GDPDFAAAFQR 60
           LE+D+ISE LIKP+ PTP HLRRY LSFIDQITVHIYAPALYFYQ N  GDPDFA  F+ 
Sbjct: 3   LEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDFRN 62

Query: 61  RLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRL 120
           RL+++LS +L+HYPPLAGRPNY ++FVDCND G  FREA   + L+DVV +A PDDLNRL
Sbjct: 63  RLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLNRL 122

Query: 121 FPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           FPVELD+ N+ELM VQFTE ACGGVAVA C+SHKIADAMTLFSLNNNWAA+
Sbjct: 123 FPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 173

[Close]

BLAST of MS000950 vs. NCBI nr
Match: XP_022951641.1 (vinorine synthase-like [Cucurbita moschata])

HSP 1 Score: 266.9 bits (681), Expect = 1.2e-67
Identity = 128/171 (74.85%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSN--GDPDFAAAFQR 60
           LE+D+ISE LIKP+ PTP HLRRY LSFIDQITVHIYAPALYFYQ N  GDPDFA  F+ 
Sbjct: 3   LEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDFRN 62

Query: 61  RLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRL 120
           RL+++LS +L+HYPPLAGRPNY ++FVDCND G  FREA   + L+DVV +A PDDLNRL
Sbjct: 63  RLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLNRL 122

Query: 121 FPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           FPVELD+ N+ELM VQFTE ACGGVAVA C+SHKIADAMTLFSLNNNWAA+
Sbjct: 123 FPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 173

[Close]

BLAST of MS000950 vs. NCBI nr
Match: XP_008445555.1 (PREDICTED: vinorine synthase-like [Cucumis melo])

HSP 1 Score: 266.5 bits (680), Expect = 1.5e-67
Identity = 130/175 (74.29%), Postives = 145/175 (82.86%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNG------DPDFAA 60
           LEVD+ISE LIKPS PTP HLR Y+LSFIDQITVHIYAPALYFYQS        D DFA 
Sbjct: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDFAL 62

Query: 61  AFQRRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDD 120
           AF+ RL+++L  +L+HYPPLAGRPNYA+SF+DCND GVPFREA   S L+DV+  A PDD
Sbjct: 63  AFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDD 122

Query: 121 LNRLFPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           LNRLFPVELDR N+ELM VQFTE ACGGVAVA C+SHKIADAM LFSLNNNWAA+
Sbjct: 123 LNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAM 177

[Close]

BLAST of MS000950 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 9.4e-28
Identity = 69/175 (39.43%), Postives = 102/175 (58.29%), Query Frame = 0


Query: 2   EVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDF-AAAFQRRL 61
           ++ ++SE LI+PS PTP  L+ + LS +DQ+ +  + P + FY +  D +   A     L
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSENL 63

Query: 62  QTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRLFP 121
           + SLS +LT + PLAGR N  +S VDCND+GVPF EA   S LS+ + +   D+LN+  P
Sbjct: 64  KRSLSTVLTQFYPLAGRININSS-VDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQYLP 123

Query: 122 V-------ELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAA 169
                   E     D  + V+ +   CGG A+  C+SHKIADA++L +  N+W A
Sbjct: 124 FQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTA 177

[Close]

BLAST of MS000950 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 3.1e-23
Identity = 67/178 (37.64%), Postives = 101/178 (56.74%), Query Frame = 0


Query: 2   EVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFY----QSNGDPDFAAAFQ 61
           +++ +SE LI PS PTP  L+ Y +S +DQ+ +  + P + FY     SN DP   A   
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDSNLDP---AQTS 63

Query: 62  RRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATP-DDLN 121
           + L+ SLS +LTH+ PLAGR N  +S VDCND+GVPF EA   + LS  + +    + L+
Sbjct: 64  QHLKQSLSKVLTHFYPLAGRINVNSS-VDCNDSGVPFVEARVQAQLSQAIQNVVELEKLD 123

Query: 122 RLFPV------ELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAA 169
           +  P       +++   D  + V+ +   CGG A+   +SHKIAD ++L +  N W A
Sbjct: 124 QYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTA 177

[Close]

BLAST of MS000950 vs. ExPASy Swiss-Prot
Match: K4D9Y4 (Acylsugar acyltransferase 3 OS=Solanum lycopersicum OX=4081 GN=ASAT3 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 4.5e-22
Identity = 58/168 (34.52%), Postives = 92/168 (54.76%), Query Frame = 0


Query: 5   VISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQRRLQTSL 64
           +IS  +IK   PTP  LR + LSF+D I   +++P  +FY      +++    + L+ SL
Sbjct: 6   IISRKMIKLLSPTPSSLRCHKLSFMDHINFPLHSPYAFFYPKI-PQNYSNKISQVLENSL 65

Query: 65  SALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRLFPVELD 124
           S +L+ Y PLAG+ N   ++VDCND G  +        +S +++H   D ++ +FP +L 
Sbjct: 66  SKVLSFYYPLAGKINNNYTYVDCNDTGAEYLNVRIDCPMSQILNHPYNDVVDVVFPQDLP 125

Query: 125 RCNDEL----MMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAA 169
             +  L    ++VQ +   CGGVAV+ C SH I D   L    N+WA+
Sbjct: 126 WSSSSLTRSPLVVQLSHFDCGGVAVSACTSHTIFDGYCLSKFINDWAS 172

[Close]

BLAST of MS000950 vs. ExPASy Swiss-Prot
Match: Q8GZU0 (Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=MAT PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 6.5e-21
Identity = 65/175 (37.14%), Postives = 90/175 (51.43%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQRRL 60
           +E + +S+ LIKPS PTP  L  Y LS+ DQ     Y  A +FY SN D    +  + +L
Sbjct: 7   VETETLSKTLIKPSSPTPQSLSHYNLSYNDQNIYPEYIFAGFFY-SNPDGHEISTIREQL 66

Query: 61  QTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRLFP 120
           Q SLS  L  Y P AG+      ++ CND G+ F +      ++D++         R + 
Sbjct: 67  QNSLSKTLVSYYPFAGKV-VKNDYIHCNDDGIEFVDVRIHCRMNDILKPEL-----RSYA 126

Query: 121 VELDRCN-------DELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAA 169
            EL R N       D   +VQ +   CGGVAVA  +SHK+ADA T+ S   +WAA
Sbjct: 127 SELIRPNRSTVGSEDSTALVQLSHFDCGGVAVAFGISHKVADAATILSFIKDWAA 174

[Close]

BLAST of MS000950 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.5e-20
Identity = 61/179 (34.08%), Postives = 94/179 (52.51%), Query Frame = 0


Query: 3   VDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFY-------QSNGDPDFAAA 62
           V V ++  IKPS  TP  LR + LSFIDQ   ++Y P  +FY       + + + D  + 
Sbjct: 10  VSVCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 63  FQRRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDL 122
               LQTSLS  L  Y P AG+    A+ VDCND G  F       ++S+++ H      
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNAT-VDCNDMGAEFLSVRIKCSMSEILDHPHASLA 129

Query: 123 NRL-FPVELDRCND----ELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
             +  P +L   N+     L++VQ ++  CGG+A++ C SHKI D  +L +  N+W+++
Sbjct: 130 ESIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSV 187

[Close]

BLAST of MS000950 vs. ExPASy TrEMBL
Match: A0A0A0LQS8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.7e-69
Identity = 131/175 (74.86%), Postives = 147/175 (84.00%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNG------DPDFAA 60
           LEVD+ISE LIKPS PTP HLR Y+LSFIDQITVHIYAPALYFYQS        D DFA 
Sbjct: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDFAL 62

Query: 61  AFQRRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDD 120
           AF+ RL+++L  +L+HYPPLAGRPNYA+SF+DCND GVPFREA+  S L+DV+  A PDD
Sbjct: 63  AFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREALVNSQLADVIQFAQPDD 122

Query: 121 LNRLFPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           LNRLFPVELDR N+ELM VQFTE ACGGVAVA C+SHKIADAMTLFSLNNNWAA+
Sbjct: 123 LNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 177

[Close]

BLAST of MS000950 vs. ExPASy TrEMBL
Match: A0A6J1GI32 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.6e-68
Identity = 128/171 (74.85%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSN--GDPDFAAAFQR 60
           LE+D+ISE LIKP+ PTP HLRRY LSFIDQITVHIYAPALYFYQ N  GDPDFA  F+ 
Sbjct: 3   LEIDIISEDLIKPATPTPPHLRRYPLSFIDQITVHIYAPALYFYQPNRAGDPDFAIDFRN 62

Query: 61  RLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRL 120
           RL+++LS +L+HYPPLAGRPNY ++FVDCND G  FREA   + L+DVV +A PDDLNRL
Sbjct: 63  RLRSTLSVVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYARPDDLNRL 122

Query: 121 FPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           FPVELD+ N+ELM VQFTE ACGGVAVA C+SHKIADAMTLFSLNNNWAA+
Sbjct: 123 FPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 173

[Close]

BLAST of MS000950 vs. ExPASy TrEMBL
Match: A0A5A7UYQ7 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001680 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 7.4e-68
Identity = 130/175 (74.29%), Postives = 145/175 (82.86%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNG------DPDFAA 60
           LEVD+ISE LIKPS PTP HLR Y+LSFIDQITVHIYAPALYFYQS        D DFA 
Sbjct: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDFAL 62

Query: 61  AFQRRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDD 120
           AF+ RL+++L  +L+HYPPLAGRPNYA+SF+DCND GVPFREA   S L+DV+  A PDD
Sbjct: 63  AFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDD 122

Query: 121 LNRLFPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           LNRLFPVELDR N+ELM VQFTE ACGGVAVA C+SHKIADAM LFSLNNNWAA+
Sbjct: 123 LNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAM 177

[Close]

BLAST of MS000950 vs. ExPASy TrEMBL
Match: A0A1S3BD01 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 7.4e-68
Identity = 130/175 (74.29%), Postives = 145/175 (82.86%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNG------DPDFAA 60
           LEVD+ISE LIKPS PTP HLR Y+LSFIDQITVHIYAPALYFYQS        D DFA 
Sbjct: 3   LEVDIISEELIKPSTPTPPHLRHYSLSFIDQITVHIYAPALYFYQSTDTGVGGEDLDFAL 62

Query: 61  AFQRRLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDD 120
           AF+ RL+++L  +L+HYPPLAGRPNYA+SF+DCND GVPFREA   S L+DV+  A PDD
Sbjct: 63  AFKNRLRSTLPDVLSHYPPLAGRPNYASSFIDCNDTGVPFREARVNSQLADVIQFAQPDD 122

Query: 121 LNRLFPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           LNRLFPVELDR N+ELM VQFTE ACGGVAVA C+SHKIADAM LFSLNNNWAA+
Sbjct: 123 LNRLFPVELDRFNEELMAVQFTEFACGGVAVASCISHKIADAMALFSLNNNWAAM 177

[Close]

BLAST of MS000950 vs. ExPASy TrEMBL
Match: A0A6J1KPC8 (vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 4.0e-66
Identity = 125/171 (73.10%), Postives = 144/171 (84.21%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSN--GDPDFAAAFQR 60
           LE+D+ISE LIKP+ PTP HLR Y LSFIDQITVHIYAPALYFY+    GDPDFA  F+ 
Sbjct: 3   LEIDIISEDLIKPATPTPPHLRHYPLSFIDQITVHIYAPALYFYRPTHAGDPDFAIHFRN 62

Query: 61  RLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRL 120
           RL+++LS +L+HYPPLAGRPNY ++FVDCND G  FREA   + L+DVV +A PDDLNRL
Sbjct: 63  RLRSTLSLVLSHYPPLAGRPNYDSAFVDCNDTGALFREARVNTKLADVVEYAQPDDLNRL 122

Query: 121 FPVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           FPVELD+ N+ELM VQFTE ACGGVAVA C+SHKIADAMTLFSLNNNWAA+
Sbjct: 123 FPVELDQFNEELMAVQFTEFACGGVAVASCISHKIADAMTLFSLNNNWAAM 173

[Close]

BLAST of MS000950 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 125.9 bits (315), Expect = 3.0e-29
Identity = 65/171 (38.01%), Postives = 101/171 (59.06%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQRRL 60
           + VDV+S  +IKPS PTP+HL+++ LS ++Q+   I+ P ++FY +N         Q  L
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNSIKPTEQLQ-ML 60

Query: 61  QTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRLFP 120
           + SLS  LTH+ PLAGR     S +DCND+G  F EA   S LS+++   + D L +L P
Sbjct: 61  KKSLSETLTHFYPLAGRLKGNIS-IDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQLIP 120

Query: 121 VELD--RCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
             +D       L++ Q +   CG +++  C+SHK+ADA ++     +WAA+
Sbjct: 121 TSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAI 169

[Close]

BLAST of MS000950 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 110.9 bits (276), Expect = 9.9e-25
Identity = 62/169 (36.69%), Postives = 94/169 (55.62%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLR-RYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQRR 60
           LE+ V S+ L+KPS    +H    + LSF+DQ+   I+ P L+FY +  +          
Sbjct: 3   LEITVTSQELVKPSPRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYHNKTNLSDKER-SDH 62

Query: 61  LQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNRLF 120
           +++SLS +L  Y PLAGR   +   V CND GV F EA     +S ++ +  P++LN+L 
Sbjct: 63  IKSSLSEILNLYYPLAGRIKNSGDVVVCNDVGVSFVEAKADCNMSQILENPNPNELNKLH 122

Query: 121 PVELDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAA 169
           P E    +D  + VQ T   CGG+A+   +SHK+ DA++     N+WAA
Sbjct: 123 PFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAA 170

[Close]

BLAST of MS000950 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 102.4 bits (254), Expect = 3.5e-22
Identity = 63/173 (36.42%), Postives = 89/173 (51.45%), Query Frame = 0


Query: 3   VDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQR---- 62
           V+++S  ++KPS PTP   R   LS +D ++  +Y  AL FY +  DP     F      
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAA--DPQNLLGFSTEETS 64

Query: 63  -RLQTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREAVTGSTLSDVVHHATPDDLNR 122
            +L+ SLS  L  + PLAGR     SFV+CND G  F EA     LS+ +    P+ L  
Sbjct: 65  LKLKKSLSKTLPIFYPLAGR--IIGSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLEL 124

Query: 123 LFPVEL---DRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWA 168
           L PVE    +     ++++Q    +CGG+ +  CVSHKI DA +L      WA
Sbjct: 125 LIPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWA 173

[Close]

BLAST of MS000950 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.0e-21
Identity = 63/175 (36.00%), Postives = 94/175 (53.71%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQRRL 60
           ++V+ IS+ +IKPS PTP++L+   LS  D I   +Y  A  FY  N D         +L
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKN-DLISQEHTSHKL 65

Query: 61  QTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREA-VTGSTLSDVVHHATPDDLNRLF 120
           +TSLS  LT + PLAGR       VDC D G  F +A V    L++ +     D L +L 
Sbjct: 66  KTSLSETLTKFYPLAGR--ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQQLL 125

Query: 121 PVE------LDRCNDELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAA 169
           P++      +      L++V+ T   CGG+A+  C++HKIADA ++ +   +WAA
Sbjct: 126 PLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAA 177

[Close]

BLAST of MS000950 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 97.1 bits (240), Expect = 1.5e-20
Identity = 63/178 (35.39%), Postives = 94/178 (52.81%), Query Frame = 0


Query: 1   LEVDVISEVLIKPSCPTPHHLRRYTLSFIDQITVHIYAPALYFYQSNGDPDFAAAFQRRL 60
           ++V+ I++ +IKPS  TP+ L+   LS  D I   +Y  A  FY +  D         +L
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFY-TKDDLISPEQSSHKL 63

Query: 61  QTSLSALLTHYPPLAGRPNYAASFVDCNDAGVPFREA-VTGSTLSDVVHHATPDDLNRLF 120
           +TSL+  LT + PLAGR       +DCND G  F +A V    LSD +       L +L 
Sbjct: 64  KTSLAETLTKFYPLAGR--IKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQQLL 123

Query: 121 PVELDRCND--------ELMMVQFTELACGGVAVACCVSHKIADAMTLFSLNNNWAAL 170
           P  LD  +D         L++V+ T   CGG+A+  C++HKIADA ++ +   +WAA+
Sbjct: 124 P--LDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAM 176

The following BLAST results are available for this feature:

BLAST of MS000950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143048.1	1.4e-68	74.86	stemmadenine O-acetyltransferase [Cucumis sativus] >KGN62351.1 hypothetical prot...	[more]
KAG7020113.1	1.2e-67	74.85	Vinorine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6585195.1	1.2e-67	74.85	Stemmadenine O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022951641.1	1.2e-67	74.85	vinorine synthase-like [Cucurbita moschata]	[more]
XP_008445555.1	1.5e-67	74.29	PREDICTED: vinorine synthase-like [Cucumis melo]	[more]

BLAST of MS000950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A2P1GIW7	9.4e-28	39.43	Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1	[more]
Q70PR7	3.1e-23	37.64	Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2	[more]
K4D9Y4	4.5e-22	34.52	Acylsugar acyltransferase 3 OS=Solanum lycopersicum OX=4081 GN=ASAT3 PE=2 SV=1	[more]
Q8GZU0	6.5e-21	37.14	Minovincinine 19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=M...	[more]
D2Y3X2	2.5e-20	34.08	Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1	[more]

BLAST of MS000950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LQS8	6.7e-69	74.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350340 PE=3 SV=1	[more]
A0A6J1GI32	5.6e-68	74.85	vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1	[more]
A0A5A7UYQ7	7.4e-68	74.29	Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5...	[more]
A0A1S3BD01	7.4e-68	74.29	vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103488534 PE=3 SV=1	[more]
A0A6J1KPC8	4.0e-66	73.10	vinorine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111496400 PE=3 SV=1	[more]

BLAST of MS000950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G26040.1	3.0e-29	38.01	HXXXD-type acyl-transferase family protein	[more]
AT1G24430.1	9.9e-25	36.69	HXXXD-type acyl-transferase family protein	[more]
AT1G24420.1	3.5e-22	36.42	HXXXD-type acyl-transferase family protein	[more]
AT4G15390.1	1.0e-21	36.00	HXXXD-type acyl-transferase family protein	[more]
AT3G30280.1	1.5e-20	35.39	HXXXD-type acyl-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003480	Transferase	PFAM	PF02458	Transferase	coord: 2..169 e-value: 6.3E-39 score: 133.8
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 1..169 e-value: 1.2E-50 score: 173.8
None	No IPR available	PANTHER	PTHR31623:SF46	ANTHRANILATE N-BENZOYLTRANSFERASE	coord: 1..169
None	No IPR available	PANTHER	PTHR31623	F21J9.9	coord: 1..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000950.1	MS000950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups