MS000949 (gene) Bitter gourd (TR) v1

Overview


Name	MS000949
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	HXXXD-type acyl-transferase family protein
Location	scaffold36: 418305 .. 418514 (+)
RNA-Seq Expression	MS000949
Synteny	MS000949

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGATTTCCTCTGTACGACGCCGATTTCGGGTGGGGAAAGCCGGCGTGGGCGGCCTCGCCGGCGCTGCCTTTCAAAAACCTCGTCGTTTTGATGGACGCGAAGTCCGGCGACGGCGCCATTGACGCTGTCGTCCATTTGAACGAACCGCACATGGCTAAGCTGGAAGCCGACGAGGAGTTTCTCAAGTTTGCTTCCCCAACTGCTTCTTCC

mRNA sequence

CGATTTCCTCTGTACGACGCCGATTTCGGGTGGGGAAAGCCGGCGTGGGCGGCCTCGCCGGCGCTGCCTTTCAAAAACCTCGTCGTTTTGATGGACGCGAAGTCCGGCGACGGCGCCATTGACGCTGTCGTCCATTTGAACGAACCGCACATGGCTAAGCTGGAAGCCGACGAGGAGTTTCTCAAGTTTGCTTCCCCAACTGCTTCTTCC

Coding sequence (CDS)

CGATTTCCTCTGTACGACGCCGATTTCGGGTGGGGAAAGCCGGCGTGGGCGGCCTCGCCGGCGCTGCCTTTCAAAAACCTCGTCGTTTTGATGGACGCGAAGTCCGGCGACGGCGCCATTGACGCTGTCGTCCATTTGAACGAACCGCACATGGCTAAGCTGGAAGCCGACGAGGAGTTTCTCAAGTTTGCTTCCCCAACTGCTTCTTCC

Protein sequence

RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEFLKFASPTASS

Homology

[Close]

BLAST of MS000949 vs. NCBI nr
Match: XP_022132064.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 148.3 bits (373), Expect = 2.5e-32
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF
Sbjct: 368 RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 427

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 428 LKFASPTASS 437

[Close]

BLAST of MS000949 vs. NCBI nr
Match: XP_038886246.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 122.5 bits (306), Expect = 1.5e-24
Identity = 55/68 (80.88%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           RFPLYDADFGWG P WAASPALPFKNLVV MD KSGDGA+DA+VHL E HM + E D EF
Sbjct: 405 RFPLYDADFGWGSPLWAASPALPFKNLVVFMDTKSGDGAVDAIVHLKESHMKRFEVDCEF 464

Query: 61  LKFASPTA 69
           LKFASPTA
Sbjct: 465 LKFASPTA 472

[Close]

BLAST of MS000949 vs. NCBI nr
Match: KAA0061033.1 (vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 121.7 bits (304), Expect = 2.5e-24
Identity = 55/70 (78.57%), Postives = 58/70 (82.86%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           R PLYDADFGWG P WAASPALPFKNL VLMD KS DGA+D +VHL E HM +LE D EF
Sbjct: 68  RLPLYDADFGWGSPVWAASPALPFKNLFVLMDGKSSDGAVDVLVHLKESHMERLEVDREF 127

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 128 LKFASPTASS 137

[Close]

BLAST of MS000949 vs. NCBI nr
Match: XP_008444409.1 (PREDICTED: vinorine synthase-like [Cucumis melo])

HSP 1 Score: 121.7 bits (304), Expect = 2.5e-24
Identity = 55/70 (78.57%), Postives = 58/70 (82.86%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           R PLYDADFGWG P WAASPALPFKNL VLMD KS DGA+D +VHL E HM +LE D EF
Sbjct: 367 RLPLYDADFGWGSPVWAASPALPFKNLFVLMDGKSSDGAVDVLVHLKESHMERLEVDREF 426

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 427 LKFASPTASS 436

[Close]

BLAST of MS000949 vs. NCBI nr
Match: XP_004143050.2 (stemmadenine O-acetyltransferase [Cucumis sativus] >KAE8652046.1 hypothetical protein Csa_018730 [Cucumis sativus])

HSP 1 Score: 117.5 bits (293), Expect = 4.7e-23
Identity = 53/70 (75.71%), Postives = 57/70 (81.43%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           R PLYDADFGWG P WA SPALPFKNL VLMD K GDGA+D +VHL E HM +L+ D EF
Sbjct: 366 RLPLYDADFGWGPPDWAGSPALPFKNLFVLMDGKFGDGAVDVLVHLKESHMERLKVDREF 425

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 426 LKFASPTASS 435

[Close]

BLAST of MS000949 vs. ExPASy Swiss-Prot
Match: Q9ZTK5 (Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.4e-09
Identity = 30/66 (45.45%), Postives = 38/66 (57.58%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDG-AIDAVVHLNEPHMAKLEADEE 60
           RFP YD DFGWGKP W        KN VV+MD   GD   I+A+V   +  M+  E +E+
Sbjct: 373 RFPFYDVDFGWGKPIWVCLFQPYIKNCVVMMDYPFGDDYGIEAIVSFEQEKMSAFEKNEQ 432

Query: 61  FLKFAS 66
            L+F S
Sbjct: 433 LLQFVS 438

[Close]

BLAST of MS000949 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.4e-09
Identity = 33/65 (50.77%), Postives = 43/65 (66.15%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALP-FKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEE 60
           RFP+YD DFGWGKP    SP  P  K+L++LMD+  GDG I+A++ + E  MA  E +EE
Sbjct: 354 RFPVYDIDFGWGKPI-LVSPFQPHVKDLILLMDSPEGDG-IEALITMEEKKMAAFEKNEE 413

Query: 61  FLKFA 65
              FA
Sbjct: 414 LRSFA 416

[Close]

BLAST of MS000949 vs. ExPASy Swiss-Prot
Match: Q5SMM8 (Putrescine hydroxycinnamoyltransferase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PHT1 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-07
Identity = 25/54 (46.30%), Postives = 34/54 (62.96%), Query Frame = 0


Query: 3   PLYDADFGWGKPAWAASPALPFKNLVVLMDAK-SGDGAIDAVVHLNEPHMAKLE 56
           PLYDADFGWG+PA+     L F  LV L+  +  GDG +D VV ++   +AK +
Sbjct: 383 PLYDADFGWGRPAFMGRACLQFSGLVYLVPGRDDGDGRLDVVVAMDPESLAKFK 436

[Close]

BLAST of MS000949 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-07
Identity = 25/57 (43.86%), Postives = 35/57 (61.40%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEAD 58
           R   YD DFGWGKP    +  +P KNLV LMD ++ DG ++A + + E  M+ L +D
Sbjct: 355 RLGFYDLDFGWGKPVSVCTTTVPMKNLVYLMDTRNEDG-MEAWISMAEDEMSMLSSD 410

[Close]

BLAST of MS000949 vs. ExPASy Swiss-Prot
Match: Q6K638 (Hydroxycinnamoyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=HCT2 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.1e-06
Identity = 19/53 (35.85%), Postives = 32/53 (60.38%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAK 54
           R P++DADFGWG+P +     + ++ L  ++ + SGDG++   + L   HM K
Sbjct: 382 RLPIHDADFGWGRPVFMGPGGIAYEGLAFVLPSASGDGSLSVAISLQAEHMEK 434

[Close]

BLAST of MS000949 vs. ExPASy TrEMBL
Match: A0A6J1BS06 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111005025 PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.2e-32
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF
Sbjct: 368 RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 427

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 428 LKFASPTASS 437

[Close]

BLAST of MS000949 vs. ExPASy TrEMBL
Match: A0A5A7UYI2 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001690 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.2e-24
Identity = 55/70 (78.57%), Postives = 58/70 (82.86%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           R PLYDADFGWG P WAASPALPFKNL VLMD KS DGA+D +VHL E HM +LE D EF
Sbjct: 68  RLPLYDADFGWGSPVWAASPALPFKNLFVLMDGKSSDGAVDVLVHLKESHMERLEVDREF 127

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 128 LKFASPTASS 137

[Close]

BLAST of MS000949 vs. ExPASy TrEMBL
Match: A0A1S3BB10 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103487746 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.2e-24
Identity = 55/70 (78.57%), Postives = 58/70 (82.86%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           R PLYDADFGWG P WAASPALPFKNL VLMD KS DGA+D +VHL E HM +LE D EF
Sbjct: 367 RLPLYDADFGWGSPVWAASPALPFKNLFVLMDGKSSDGAVDVLVHLKESHMERLEVDREF 426

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 427 LKFASPTASS 436

[Close]

BLAST of MS000949 vs. ExPASy TrEMBL
Match: A0A0A0LKB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350330 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.3e-23
Identity = 53/70 (75.71%), Postives = 57/70 (81.43%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           R PLYDADFGWG P WA SPALPFKNL VLMD K GDGA+D +VHL E HM +L+ D EF
Sbjct: 68  RLPLYDADFGWGPPDWAGSPALPFKNLFVLMDGKFGDGAVDVLVHLKESHMERLKVDREF 127

Query: 61  LKFASPTASS 71
           LKFASPTASS
Sbjct: 128 LKFASPTASS 137

[Close]

BLAST of MS000949 vs. ExPASy TrEMBL
Match: A0A6J1GI32 (vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 9.9e-19
Identity = 45/70 (64.29%), Postives = 55/70 (78.57%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           RFPLYD DFGWG+P W   PAL FKNLV  +D+K  DG IDA+VHL EPHM KLEAD+ F
Sbjct: 365 RFPLYDVDFGWGRPVWVTFPALWFKNLVTFLDSKE-DGGIDAIVHLEEPHMKKLEADQLF 424

Query: 61  LKFASPTASS 71
           L++A+PT ++
Sbjct: 425 LQYATPTPTA 433

[Close]

BLAST of MS000949 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 81.3 bits (199), Expect = 3.5e-16
Identity = 35/69 (50.72%), Postives = 50/69 (72.46%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           +FP+Y+ADFGWGKP W AS  + +KNLV  +D K GDG I+A ++L++  M++ EADEE 
Sbjct: 362 KFPVYEADFGWGKPLWVASARMSYKNLVAFIDTKEGDG-IEAWINLDQNDMSRFEADEEL 421

Query: 61  LKFASPTAS 70
           L++ S   S
Sbjct: 422 LRYVSSNPS 429

[Close]

BLAST of MS000949 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 72.0 bits (175), Expect = 2.1e-13
Identity = 34/65 (52.31%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           RF LY+ DFGWGKP W   P++  KN+V L+D K   G I+A V+LNE  M   E D E 
Sbjct: 366 RFGLYETDFGWGKPVWVGFPSVRQKNIVTLLDTKEA-GGIEAWVNLNEQEMNLFEQDREL 425

Query: 61  LKFAS 66
           L+FAS
Sbjct: 426 LQFAS 429

[Close]

BLAST of MS000949 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 65.1 bits (157), Expect = 2.6e-11
Identity = 32/63 (50.79%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 4   LYDADFGWGKPAWAASPALP-FKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEFLK 63
           LYDADFGWGKP W        FKNL++L+D K G+G I+A + L E  M+  E D+E L+
Sbjct: 368 LYDADFGWGKPVWVTGRGTSHFKNLMLLIDTKDGEG-IEAWITLTEEQMSLFECDQELLE 427

Query: 64  FAS 66
            AS
Sbjct: 428 SAS 429

[Close]

BLAST of MS000949 vs. TAIR 10
Match: AT5G23970.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 63.5 bits (153), Expect = 7.5e-11
Identity = 33/69 (47.83%), Postives = 44/69 (63.77%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALPFKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEEF 60
           R   Y+A+FGWGKP W A  ++  K  VVLMD+K  +G ++A V L E  MAK E D E 
Sbjct: 359 RMSFYEANFGWGKPVWVAPDSVD-KTQVVLMDSKDSEG-VEARVTLPETDMAKYEHDSEL 418

Query: 61  LKFASPTAS 70
           L +A+P+ S
Sbjct: 419 LVYATPSPS 425

[Close]

BLAST of MS000949 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.7e-10
Identity = 32/70 (45.71%), Postives = 42/70 (60.00%), Query Frame = 0


Query: 1   RFPLYDADFGWGKPAWAASPALP-FKNLVVLMDAKSGDGAIDAVVHLNEPHMAKLEADEE 60
           + PLY+A FGWG P W A    P  +N+ +L+D+K G G I+A V L E +M  LE + E
Sbjct: 372 KLPLYEASFGWGSPVWIAGNVAPMLENVTMLIDSKDGQG-IEAFVTLPEENMLSLEQNTE 431

Query: 61  FLKFASPTAS 70
            L FAS   S
Sbjct: 432 LLAFASVNPS 440

The following BLAST results are available for this feature:

BLAST of MS000949 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132064.1	2.5e-32	100.00	vinorine synthase-like [Momordica charantia]	[more]
XP_038886246.1	1.5e-24	80.88	stemmadenine O-acetyltransferase-like [Benincasa hispida]	[more]
KAA0061033.1	2.5e-24	78.57	vinorine synthase-like [Cucumis melo var. makuwa]	[more]
XP_008444409.1	2.5e-24	78.57	PREDICTED: vinorine synthase-like [Cucumis melo]	[more]
XP_004143050.2	4.7e-23	75.71	stemmadenine O-acetyltransferase [Cucumis sativus] >KAE8652046.1 hypothetical pr...	[more]

BLAST of MS000949 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZTK5	1.4e-09	45.45	Deacetylvindoline O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=DAT PE=1...	[more]
A0A2K8FQU5	1.4e-09	50.77	Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT...	[more]
Q5SMM8	3.8e-07	46.30	Putrescine hydroxycinnamoyltransferase 1 OS=Oryza sativa subsp. japonica OX=3994...	[more]
A0A2P1GIW7	3.8e-07	43.86	Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1	[more]
Q6K638	1.1e-06	35.85	Hydroxycinnamoyltransferase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=HCT2 P...	[more]

BLAST of MS000949 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BS06	1.2e-32	100.00	vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111005025 PE=3 SV=1	[more]
A0A5A7UYI2	1.2e-24	78.57	Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5...	[more]
A0A1S3BB10	1.2e-24	78.57	vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103487746 PE=3 SV=1	[more]
A0A0A0LKB2	2.3e-23	75.71	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350330 PE=3 SV=1	[more]
A0A6J1GI32	9.9e-19	64.29	vinorine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111454395 PE=3 SV=1	[more]

BLAST of MS000949 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24430.1	3.5e-16	50.72	HXXXD-type acyl-transferase family protein	[more]
AT3G26040.1	2.1e-13	52.31	HXXXD-type acyl-transferase family protein	[more]
AT1G24420.1	2.6e-11	50.79	HXXXD-type acyl-transferase family protein	[more]
AT5G23970.1	7.5e-11	47.83	HXXXD-type acyl-transferase family protein	[more]
AT3G30280.1	1.7e-10	45.71	HXXXD-type acyl-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 1..63 e-value: 1.7E-20 score: 75.1
IPR003480	Transferase	PFAM	PF02458	Transferase	coord: 1..60 e-value: 2.5E-12 score: 46.2
None	No IPR available	PANTHER	PTHR31623	F21J9.9	coord: 1..66
None	No IPR available	PANTHER	PTHR31623:SF46	ANTHRANILATE N-BENZOYLTRANSFERASE	coord: 1..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000949.1	MS000949.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups