MS000948 (gene) Bitter gourd (TR) v1

Overview


Name	MS000948
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	HXXXD-type acyl-transferase family protein, putative
Location	scaffold36: 416225 .. 416791 (+)
RNA-Seq Expression	MS000948
Synteny	MS000948

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGAAGTTGAAGTAATCTCAAAGGAGTTTGTCAAACCTTCTTCTCCGACGCCGGAAAATCTCCGCCGTTACAAATTCTCCTTCCTCGACCAAGTCACCGCCGATGTCTATAATCCCATGGTCTATTTCTACGAAGCGCTCGACAGCGGTCCAAATTTCAACGCCGTCGAAATTAAAAACCGCCTCAAAAACTCCCTCTCCCACGTCTTGTCGAGCTACTATCCCCTCGCCGGCCGACACCACAACGACGCCGATTCTTTCATCGATTGCAACGACGACGGCGTTCCTTTCCTGGAAGCACGCGTCAAATCCAACCTCTACGACGTCGTTCGCCACTCCTCTCCCGCGGATCTCAACGACCTCCTCCCCTTCCAGTTGGATCAACTCAACGAAATTTCCATGGGCGTTCAATTTAACGTGTTCGAATTCGGCGGAATCGCCCTCGCGATTTGCGTGTCGCACAAAATTGCCGACGCTCTGTCGTTTTTCTCCGTCGTGAACGGCTGGGCGGTGGCCGCACGCGGCGCACCGGAGGCTCTCGGGCGGCCTCATATTGCGGCGGCGAAG

mRNA sequence

CTTGAAGTTGAAGTAATCTCAAAGGAGTTTGTCAAACCTTCTTCTCCGACGCCGGAAAATCTCCGCCGTTACAAATTCTCCTTCCTCGACCAAGTCACCGCCGATGTCTATAATCCCATGGTCTATTTCTACGAAGCGCTCGACAGCGGTCCAAATTTCAACGCCGTCGAAATTAAAAACCGCCTCAAAAACTCCCTCTCCCACGTCTTGTCGAGCTACTATCCCCTCGCCGGCCGACACCACAACGACGCCGATTCTTTCATCGATTGCAACGACGACGGCGTTCCTTTCCTGGAAGCACGCGTCAAATCCAACCTCTACGACGTCGTTCGCCACTCCTCTCCCGCGGATCTCAACGACCTCCTCCCCTTCCAGTTGGATCAACTCAACGAAATTTCCATGGGCGTTCAATTTAACGTGTTCGAATTCGGCGGAATCGCCCTCGCGATTTGCGTGTCGCACAAAATTGCCGACGCTCTGTCGTTTTTCTCCGTCGTGAACGGCTGGGCGGTGGCCGCACGCGGCGCACCGGAGGCTCTCGGGCGGCCTCATATTGCGGCGGCGAAG

Coding sequence (CDS)

CTTGAAGTTGAAGTAATCTCAAAGGAGTTTGTCAAACCTTCTTCTCCGACGCCGGAAAATCTCCGCCGTTACAAATTCTCCTTCCTCGACCAAGTCACCGCCGATGTCTATAATCCCATGGTCTATTTCTACGAAGCGCTCGACAGCGGTCCAAATTTCAACGCCGTCGAAATTAAAAACCGCCTCAAAAACTCCCTCTCCCACGTCTTGTCGAGCTACTATCCCCTCGCCGGCCGACACCACAACGACGCCGATTCTTTCATCGATTGCAACGACGACGGCGTTCCTTTCCTGGAAGCACGCGTCAAATCCAACCTCTACGACGTCGTTCGCCACTCCTCTCCCGCGGATCTCAACGACCTCCTCCCCTTCCAGTTGGATCAACTCAACGAAATTTCCATGGGCGTTCAATTTAACGTGTTCGAATTCGGCGGAATCGCCCTCGCGATTTGCGTGTCGCACAAAATTGCCGACGCTCTGTCGTTTTTCTCCGTCGTGAACGGCTGGGCGGTGGCCGCACGCGGCGCACCGGAGGCTCTCGGGCGGCCTCATATTGCGGCGGCGAAG

Protein sequence

LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKNRLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLNDLLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEALGRPHIAAAK

Homology

[Close]

BLAST of MS000948 vs. NCBI nr
Match: XP_022132064.1 (vinorine synthase-like [Momordica charantia])

HSP 1 Score: 370.9 bits (951), Expect = 6.4e-99
Identity = 184/189 (97.35%), Postives = 185/189 (97.88%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LEVEVISKEFV+PSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALD GPNFNAVEIKN
Sbjct: 3   LEVEVISKEFVRPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDGGPNFNAVEIKN 62

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
           RLKNSLS VLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND
Sbjct: 63  RLKNSLSQVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 122

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           LLPFQLDQLNEISMGVQFNVFE GGIALAICVSHKIADALSFFSVVNGWAVAARGA EAL
Sbjct: 123 LLPFQLDQLNEISMGVQFNVFELGGIALAICVSHKIADALSFFSVVNGWAVAARGASEAL 182

Query: 181 GRPHIAAAK 190
           GRPHIAAAK
Sbjct: 183 GRPHIAAAK 191

[Close]

BLAST of MS000948 vs. NCBI nr
Match: KAA0061034.1 (vinorine synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 274.6 bits (701), Expect = 6.2e-70
Identity = 142/189 (75.13%), Postives = 153/189 (80.95%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LE EVIS+EFVKPSSPTP NLRRYKFSFLDQVTADVYNPMVYF+E      NF  +EIKN
Sbjct: 24  LEFEVISQEFVKPSSPTPANLRRYKFSFLDQVTADVYNPMVYFFELNGDYSNFTEIEIKN 83

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LK+SLS VLS YYPLAGR  N AD  IDCNDDGVPFLE RVKS L+DV+  S P DLN 
Sbjct: 84  HLKHSLSAVLSDYYPLAGR-VNYADFEIDCNDDGVPFLETRVKSKLHDVISKSFPEDLNC 143

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           L+PF+LD+LNEISMGVQ N FE GGI L +CVSHKIADALSFFS VN WA AARG  EAL
Sbjct: 144 LVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSAVNIWAKAARGEVEAL 203

Query: 181 GRPHIAAAK 190
           GRPH+ AAK
Sbjct: 204 GRPHLVAAK 211

[Close]

BLAST of MS000948 vs. NCBI nr
Match: XP_008444409.1 (PREDICTED: vinorine synthase-like [Cucumis melo])

HSP 1 Score: 274.6 bits (701), Expect = 6.2e-70
Identity = 142/189 (75.13%), Postives = 153/189 (80.95%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LE EVIS+EFVKPSSPTP NLRRYKFSFLDQVTADVYNPMVYF+E      NF  +EIKN
Sbjct: 3   LEFEVISQEFVKPSSPTPANLRRYKFSFLDQVTADVYNPMVYFFELNGDYSNFTEIEIKN 62

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LK+SLS VLS YYPLAGR  N AD  IDCNDDGVPFLE RVKS L+DV+  S P DLN 
Sbjct: 63  HLKHSLSAVLSDYYPLAGR-VNYADFEIDCNDDGVPFLETRVKSKLHDVISKSFPEDLNC 122

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           L+PF+LD+LNEISMGVQ N FE GGI L +CVSHKIADALSFFS VN WA AARG  EAL
Sbjct: 123 LVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSAVNIWAKAARGEVEAL 182

Query: 181 GRPHIAAAK 190
           GRPH+ AAK
Sbjct: 183 GRPHLVAAK 190

[Close]

BLAST of MS000948 vs. NCBI nr
Match: XP_038886246.1 (stemmadenine O-acetyltransferase-like [Benincasa hispida])

HSP 1 Score: 271.9 bits (694), Expect = 4.0e-69
Identity = 139/189 (73.54%), Postives = 154/189 (81.48%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LE+EV S+E VKPSSPTP NLRRYK SFLDQVTADVYNPMVYFY+ L+   NF  ++IKN
Sbjct: 42  LELEVTSEELVKPSSPTPANLRRYKLSFLDQVTADVYNPMVYFYQ-LNRDSNFTKIQIKN 101

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LKN+LS VLS YYPLAGR  N AD  IDCNDDG+PFLE RVK NL+DVV  S P DLN 
Sbjct: 102 HLKNTLSAVLSDYYPLAGR-VNYADFVIDCNDDGIPFLETRVKFNLHDVVSRSFPEDLNC 161

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           L+PF+LD+LNEISMGVQ N FE GGI L +CVSHKIADALSFFSVVN WA AARG  EA+
Sbjct: 162 LVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSVVNSWARAARGEAEAI 221

Query: 181 GRPHIAAAK 190
           GRPH+ AAK
Sbjct: 222 GRPHLVAAK 228

[Close]

BLAST of MS000948 vs. NCBI nr
Match: XP_004143050.2 (stemmadenine O-acetyltransferase [Cucumis sativus] >KAE8652046.1 hypothetical protein Csa_018730 [Cucumis sativus])

HSP 1 Score: 261.5 bits (667), Expect = 5.5e-66
Identity = 138/189 (73.02%), Postives = 149/189 (78.84%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LE EVISKE VKPSSPTP NLRRYKFSFLDQVTADVYNPMVYF++ L+   NF  +EIK 
Sbjct: 3   LEFEVISKELVKPSSPTPPNLRRYKFSFLDQVTADVYNPMVYFFQ-LNGDSNFTEIEIKT 62

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LK SLS VLS YYPLAGR  N AD  IDCND GVPFLE R+KS L+DV   S P DLN 
Sbjct: 63  HLKTSLSAVLSDYYPLAGR-VNYADFEIDCNDHGVPFLETRIKSTLHDVFSMSFPEDLNC 122

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           L+PF+LD+LNE SMGVQ N FE GGI L +CVSHKIADALSFFSVVN WA AARG  EAL
Sbjct: 123 LVPFELDRLNETSMGVQLNFFECGGIGLGVCVSHKIADALSFFSVVNIWARAARGEAEAL 182

Query: 181 GRPHIAAAK 190
           GR H+ AAK
Sbjct: 183 GRAHLVAAK 189

[Close]

BLAST of MS000948 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 2.8e-33
Identity = 76/181 (41.99%), Postives = 113/181 (62.43%), Query Frame = 0


Query: 2   EVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFY-EALDSGPNFNAVEIKN 61
           +++++S+E ++PSSPTP+ L+ +K S LDQV    + P++ FY   LDS  N +  +   
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDS--NLDRAQRSE 63

Query: 62  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 121
            LK SLS VL+ +YPLAGR   + +S +DCND GVPFLEARV S L + +++ +  +LN 
Sbjct: 64  NLKRSLSTVLTQFYPLAGR--ININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQ 123

Query: 122 LLPFQ-------LDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAA 175
            LPFQ            +I + V+ + FE GG A+ +C+SHKIADALS  + +N W    
Sbjct: 124 YLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATC 180

[Close]

BLAST of MS000948 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 129.0 bits (323), Expect = 5.6e-29
Identity = 72/185 (38.92%), Postives = 111/185 (60.00%), Query Frame = 0


Query: 2   EVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFY-EALDSGPNFNAVEIKN 61
           ++E +S+E + PSSPTP++L+ YK S LDQ+    + P + FY   LDS  N +  +   
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYPNPLDS--NLDPAQTSQ 63

Query: 62  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPAD-LN 121
            LK SLS VL+ +YPLAGR   + +S +DCND GVPF+EARV++ L   +++    + L+
Sbjct: 64  HLKQSLSKVLTHFYPLAGR--INVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLD 123

Query: 122 DLLPF------QLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAA 179
             LP       +++   ++ + V+ + FE GG A+ + +SHKIAD LS  + +N W    
Sbjct: 124 QYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTATC 183

[Close]

BLAST of MS000948 vs. ExPASy Swiss-Prot
Match: A0A2K8FQU5 (Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 68/170 (40.00%), Postives = 101/170 (59.41%), Query Frame = 0


Query: 3   VEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKNRL 62
           VE++SK F+KPSSPTP++LRRY  S +DQ        + +FY      P+  + +I + L
Sbjct: 6   VEIVSKMFIKPSSPTPQSLRRYNLSSIDQTIDSEVTSLAFFY---TYNPSHESSKIGDLL 65

Query: 63  KNSLSHVLSSYYPLAGR-HHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLNDL 122
           KNSLS  L SYY  AGR   ND   +IDCND+GV F+E R+   + D+++       + +
Sbjct: 66  KNSLSKTLVSYYQFAGRLIEND---YIDCNDEGVEFVEVRIHGRMNDILKRGKSFATDLV 125

Query: 123 LPFQLDQLNEIS-MGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWA 171
           LP ++  L+E S + VQ + F+ GGIA+    SHK++D +S    +  WA
Sbjct: 126 LPTRIIALHEDSLLIVQLSHFDCGGIAIGFGASHKVSDGVSNVMFMKDWA 169

[Close]

BLAST of MS000948 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.0e-26
Identity = 72/183 (39.34%), Postives = 106/183 (57.92%), Query Frame = 0


Query: 3   VEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAV--EIKN 62
           VEVISKE +KP +PTP  LR Y  S LDQ ++ VY P++ FY A     +  +   +  +
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDDLH 68

Query: 63  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 122
            LK SLS  L  +YP+AGR  ++    +DCND+G+ F E R+K  + D +   S A L+ 
Sbjct: 69  LLKRSLSETLVHFYPMAGRMKDNMT--VDCNDEGIDFFEVRIKGRMCDFMM-KSDAHLSL 128

Query: 123 LLPFQLDQLN---EISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWA----VAA 177
           LLP ++   N   E  + VQ N+F+ GG A+  C+S+KIADA +  + +   A    +A 
Sbjct: 129 LLPSEVASTNFVKEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIAR 188

[Close]

BLAST of MS000948 vs. ExPASy Swiss-Prot
Match: D2Y3X2 (Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.2e-25
Identity = 68/182 (37.36%), Postives = 100/182 (54.95%), Query Frame = 0


Query: 3   VEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEAL-----DSGPNFNAVE 62
           V V +K F+KPSS TP  LR +K SF+DQ  +++Y P  +FY  +     DS  +     
Sbjct: 10  VSVCNKSFIKPSSLTPSTLRFHKLSFIDQSLSNMYIPCAFFYPKVQQRLEDSKNSDELSH 69

Query: 63  IKNRLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPAD 122
           I + L+ SLS  L SYYP AG+  ++A   +DCND G  FL  R+K ++ +++ H   + 
Sbjct: 70  IAHLLQTSLSQTLVSYYPYAGKLKDNAT--VDCNDMGAEFLSVRIKCSMSEILDHPHASL 129

Query: 123 LNDL-LPFQLDQLNEISMG----VQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVA 175
              + LP  L   N    G    VQ + F+ GGIA+++C SHKI D  S  + +N W+  
Sbjct: 130 AESIVLPKDLPWANNCEGGNLLVVQVSKFDCGGIAISVCFSHKIGDGCSLLNFLNDWSSV 189

[Close]

BLAST of MS000948 vs. ExPASy TrEMBL
Match: A0A6J1BS06 (vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111005025 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.1e-99
Identity = 184/189 (97.35%), Postives = 185/189 (97.88%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LEVEVISKEFV+PSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALD GPNFNAVEIKN
Sbjct: 3   LEVEVISKEFVRPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDGGPNFNAVEIKN 62

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
           RLKNSLS VLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND
Sbjct: 63  RLKNSLSQVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 122

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           LLPFQLDQLNEISMGVQFNVFE GGIALAICVSHKIADALSFFSVVNGWAVAARGA EAL
Sbjct: 123 LLPFQLDQLNEISMGVQFNVFELGGIALAICVSHKIADALSFFSVVNGWAVAARGASEAL 182

Query: 181 GRPHIAAAK 190
           GRPHIAAAK
Sbjct: 183 GRPHIAAAK 191

[Close]

BLAST of MS000948 vs. ExPASy TrEMBL
Match: A0A5A7V131 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001700 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.0e-70
Identity = 142/189 (75.13%), Postives = 153/189 (80.95%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LE EVIS+EFVKPSSPTP NLRRYKFSFLDQVTADVYNPMVYF+E      NF  +EIKN
Sbjct: 24  LEFEVISQEFVKPSSPTPANLRRYKFSFLDQVTADVYNPMVYFFELNGDYSNFTEIEIKN 83

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LK+SLS VLS YYPLAGR  N AD  IDCNDDGVPFLE RVKS L+DV+  S P DLN 
Sbjct: 84  HLKHSLSAVLSDYYPLAGR-VNYADFEIDCNDDGVPFLETRVKSKLHDVISKSFPEDLNC 143

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           L+PF+LD+LNEISMGVQ N FE GGI L +CVSHKIADALSFFS VN WA AARG  EAL
Sbjct: 144 LVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSAVNIWAKAARGEVEAL 203

Query: 181 GRPHIAAAK 190
           GRPH+ AAK
Sbjct: 204 GRPHLVAAK 211

[Close]

BLAST of MS000948 vs. ExPASy TrEMBL
Match: A0A1S3BB10 (vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103487746 PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.0e-70
Identity = 142/189 (75.13%), Postives = 153/189 (80.95%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LE EVIS+EFVKPSSPTP NLRRYKFSFLDQVTADVYNPMVYF+E      NF  +EIKN
Sbjct: 3   LEFEVISQEFVKPSSPTPANLRRYKFSFLDQVTADVYNPMVYFFELNGDYSNFTEIEIKN 62

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LK+SLS VLS YYPLAGR  N AD  IDCNDDGVPFLE RVKS L+DV+  S P DLN 
Sbjct: 63  HLKHSLSAVLSDYYPLAGR-VNYADFEIDCNDDGVPFLETRVKSKLHDVISKSFPEDLNC 122

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           L+PF+LD+LNEISMGVQ N FE GGI L +CVSHKIADALSFFS VN WA AARG  EAL
Sbjct: 123 LVPFELDRLNEISMGVQLNFFECGGIGLGVCVSHKIADALSFFSAVNIWAKAARGEVEAL 182

Query: 181 GRPHIAAAK 190
           GRPH+ AAK
Sbjct: 183 GRPHLVAAK 190

[Close]

BLAST of MS000948 vs. ExPASy TrEMBL
Match: A0A0A0LMW5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350320 PE=3 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.6e-66
Identity = 138/189 (73.02%), Postives = 149/189 (78.84%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           LE EVISKE VKPSSPTP NLRRYKFSFLDQVTADVYNPMVYF++ L+   NF  +EIK 
Sbjct: 3   LEFEVISKELVKPSSPTPPNLRRYKFSFLDQVTADVYNPMVYFFQ-LNGDSNFTEIEIKT 62

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LK SLS VLS YYPLAGR  N AD  IDCND GVPFLE R+KS L+DV   S P DLN 
Sbjct: 63  HLKTSLSAVLSDYYPLAGR-VNYADFEIDCNDHGVPFLETRIKSTLHDVFSMSFPEDLNC 122

Query: 121 LLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEAL 180
           L+PF+LD+LNE SMGVQ N FE GGI L +CVSHKIADALSFFSVVN WA AARG  EAL
Sbjct: 123 LVPFELDRLNETSMGVQLNFFECGGIGLGVCVSHKIADALSFFSVVNIWARAARGEAEAL 182

Query: 181 GRPHIAAAK 190
           GR H+ AAK
Sbjct: 183 GRAHLVAAK 189

[Close]

BLAST of MS000948 vs. ExPASy TrEMBL
Match: A0A5D3CZ30 (Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001940 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.5e-56
Identity = 116/188 (61.70%), Postives = 145/188 (77.13%), Query Frame = 0


Query: 2   EVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKNR 61
           +++++SKE +KPSSPTP++LRRY  SFLDQVT DVYNPMVYF+ A   G      EI +R
Sbjct: 4   QIQIVSKETIKPSSPTPDHLRRYSLSFLDQVTVDVYNPMVYFFVA---GSTTTPAEIADR 63

Query: 62  LKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLNDL 121
           LK SLS VL+ YYPLAGR + D + FIDCNDDGVPF+E RV   L DV+    P++LN L
Sbjct: 64  LKTSLSDVLTHYYPLAGRLNYD-EFFIDCNDDGVPFIETRVNCPLSDVMNTPFPSELNRL 123

Query: 122 LPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGAPEALG 181
           LPF+LD+L+++SMGVQ NVFE GG+A+ ICVSHKI+DALSFF VVNGWA   RG  EAL 
Sbjct: 124 LPFELDKLDDVSMGVQLNVFECGGVAVGICVSHKISDALSFFIVVNGWAAYCRGEKEAL- 183

Query: 182 RPHIAAAK 190
           R H+++A+
Sbjct: 184 RAHLSSAE 186

[Close]

BLAST of MS000948 vs. TAIR 10
Match: AT1G24430.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 137.9 bits (346), Expect = 8.5e-33
Identity = 77/193 (39.90%), Postives = 113/193 (58.55%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLR----RYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAV 60
           LE+ V S+E VKPS   P NL      +  SFLDQ+   ++ P ++FY    +  N +  
Sbjct: 3   LEITVTSQELVKPS---PRNLNHPPCHHHLSFLDQLAPPIFMPFLFFYH---NKTNLSDK 62

Query: 61  EIKNRLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPA 120
           E  + +K+SLS +L+ YYPLAGR  N  D  + CND GV F+EA+   N+  ++ + +P 
Sbjct: 63  ERSDHIKSSLSEILNLYYPLAGRIKNSGDVVV-CNDVGVSFVEAKADCNMSQILENPNPN 122

Query: 121 DLNDLLPFQLDQLNEISMGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARGA 180
           +LN L PF+  +++++ + VQ   FE GG+AL I +SHK+ DALS    VN WA  ARG 
Sbjct: 123 ELNKLHPFEFHEVSDVPLTVQLTFFECGGLALGIGLSHKLCDALSGLIFVNSWAAFARGQ 182

Query: 181 PEALGRPHIAAAK 190
            + +  P    AK
Sbjct: 183 TDEIITPSFDLAK 188

[Close]

BLAST of MS000948 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 136.0 bits (341), Expect = 3.2e-32
Identity = 72/186 (38.71%), Postives = 113/186 (60.75%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           + V+V+S++ +KPSSPTP +L+++K S L+Q+   ++ PMV+FY A +S       E   
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFYSANNS---IKPTEQLQ 60

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLND 120
            LK SLS  L+ +YPLAGR   +    IDCND G  FLEARV S L +++   S   L  
Sbjct: 61  MLKKSLSETLTHFYPLAGRLKGNIS--IDCNDSGADFLEARVNSPLSNLLLEPSSDSLQQ 120

Query: 121 LLPFQLDQLNEIS--MGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGW-AVAARGAP 180
           L+P  +D +   +  +  Q + FE G +++ +C+SHK+ADA S    +  W A+++RG+ 
Sbjct: 121 LIPTSVDSIETRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSRGSI 180

Query: 181 EALGRP 184
           + +G P
Sbjct: 181 KTIGAP 181

[Close]

BLAST of MS000948 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 124.4 bits (311), Expect = 9.7e-29
Identity = 70/177 (39.55%), Postives = 101/177 (57.06%), Query Frame = 0


Query: 3   VEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSG-PNFNAVEIKNR 62
           VE++S+E VKPSSPTP++ R    S LD +++ +Y   + FY A       F+  E   +
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYAADPQNLLGFSTEETSLK 64

Query: 63  LKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKSNLYDVVRHSSPADLNDL 122
           LK SLS  L  +YPLAGR      SF++CND+G  F+EARV   L + ++   P  L  L
Sbjct: 65  LKKSLSKTLPIFYPLAGR---IIGSFVECNDEGAVFIEARVDHLLSEFLKCPVPESLELL 124

Query: 123 LPFQLDQLNEIS---MGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAARG 176
           +P +      ++   + +Q N F  GG+ + ICVSHKI DA S    + GWA ++RG
Sbjct: 125 IPVEAKSREAVTWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWAESSRG 178

[Close]

BLAST of MS000948 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 109.4 bits (272), Expect = 3.2e-24
Identity = 72/196 (36.73%), Postives = 105/196 (53.57%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           ++VE I+KE +KPSS TP +L+  + S  D +   VY     FY   D     +  +  +
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFYTKDDL---ISPEQSSH 63

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKS-NLYDVVRHSSPADLN 120
           +LK SL+  L+ +YPLAGR        IDCND+G  F++ARV +  L D +R      L 
Sbjct: 64  KLKTSLAETLTKFYPLAGRIKGVT---IDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQ 123

Query: 121 DLLPFQ-LDQLNEIS-----MGVQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAA 180
            LLP   +D   E +     + V+   F  GG+A+ +C++HKIADA S  + +  WA  A
Sbjct: 124 QLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMA 183

Query: 181 RG-APEALGRPHIAAA 189
           RG A + +  P  AAA
Sbjct: 184 RGEAGDIVADPVFAAA 193

[Close]

BLAST of MS000948 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 109.0 bits (271), Expect = 4.2e-24
Identity = 67/186 (36.02%), Postives = 97/186 (52.15%), Query Frame = 0


Query: 1   LEVEVISKEFVKPSSPTPENLRRYKFSFLDQVTADVYNPMVYFYEALDSGPNFNAVEIKN 60
           ++VE ISKE +KPSSPTP NL+  + S  D +   VY     FY   D     +     +
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFYTKNDL---ISQEHTSH 65

Query: 61  RLKNSLSHVLSSYYPLAGRHHNDADSFIDCNDDGVPFLEARVKS-NLYDVVRHSSPADLN 120
           +LK SLS  L+ +YPLAGR        +DC D+G  F++ARV +  L + ++      L 
Sbjct: 66  KLKTSLSETLTKFYPLAGR---ITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQ 125

Query: 121 DLLPFQLDQLNEISMG------VQFNVFEFGGIALAICVSHKIADALSFFSVVNGWAVAA 180
            LLP  +     ++        V+   F  GG+A+ IC++HKIADA S  + +  WA  A
Sbjct: 126 QLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATA 185

The following BLAST results are available for this feature:

BLAST of MS000948 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132064.1	6.4e-99	97.35	vinorine synthase-like [Momordica charantia]	[more]
KAA0061034.1	6.2e-70	75.13	vinorine synthase-like [Cucumis melo var. makuwa]	[more]
XP_008444409.1	6.2e-70	75.13	PREDICTED: vinorine synthase-like [Cucumis melo]	[more]
XP_038886246.1	4.0e-69	73.54	stemmadenine O-acetyltransferase-like [Benincasa hispida]	[more]
XP_004143050.2	5.5e-66	73.02	stemmadenine O-acetyltransferase [Cucumis sativus] >KAE8652046.1 hypothetical pr...	[more]

BLAST of MS000948 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A2P1GIW7	2.8e-33	41.99	Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1	[more]
Q70PR7	5.6e-29	38.92	Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2	[more]
A0A2K8FQU5	1.5e-26	40.00	Tabersonine-19-hydroxy-O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=TAT...	[more]
I3PLR4	2.0e-26	39.34	(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ...	[more]
D2Y3X2	2.2e-25	37.36	Acyltransferase Pun1 OS=Capsicum annuum OX=4072 GN=PUN1 PE=1 SV=1	[more]

BLAST of MS000948 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BS06	3.1e-99	97.35	vinorine synthase-like OS=Momordica charantia OX=3673 GN=LOC111005025 PE=3 SV=1	[more]
A0A5A7V131	3.0e-70	75.13	Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5...	[more]
A0A1S3BB10	3.0e-70	75.13	vinorine synthase-like OS=Cucumis melo OX=3656 GN=LOC103487746 PE=3 SV=1	[more]
A0A0A0LMW5	2.6e-66	73.02	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G350320 PE=3 SV=1	[more]
A0A5D3CZ30	1.5e-56	61.70	Vinorine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3...	[more]

BLAST of MS000948 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24430.1	8.5e-33	39.90	HXXXD-type acyl-transferase family protein	[more]
AT3G26040.1	3.2e-32	38.71	HXXXD-type acyl-transferase family protein	[more]
AT1G24420.1	9.7e-29	39.55	HXXXD-type acyl-transferase family protein	[more]
AT3G30280.1	3.2e-24	36.73	HXXXD-type acyl-transferase family protein	[more]
AT4G15390.1	4.2e-24	36.02	HXXXD-type acyl-transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003480	Transferase	PFAM	PF02458	Transferase	coord: 2..179 e-value: 1.5E-40 score: 139.2
IPR023213	Chloramphenicol acetyltransferase-like domain superfamily	GENE3D	3.30.559.10		coord: 1..189 e-value: 1.9E-52 score: 179.7
None	No IPR available	PANTHER	PTHR31623	F21J9.9	coord: 1..189
None	No IPR available	PANTHER	PTHR31623:SF46	ANTHRANILATE N-BENZOYLTRANSFERASE	coord: 1..189

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000948.1	MS000948.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups