MS000933 (gene) Bitter gourd (TR) v1

Overview


Name	MS000933
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	vignain-like
Location	scaffold36: 342295 .. 343756 (+)
RNA-Seq Expression	MS000933
Synteny	MS000933

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATTGTCAAATTTCTCATGGTTCCTCTTCTTCTGTTTGTTCTAGTTTCAGGATTGGCCGAGAGCTTTGAATTCAATGAGACGGAATTAGCATCAGAACAGAGTCTATGGAAGCTGTATGAGAGGTGGAGTAACCACCATACAATCTCAAGGGGCCTAAAGGAGAAGCACAGACGTTTCAGTGTGTTCAAAGAGAATGTGAACCATGTGTTCACAGCGAACCAAATGGAAAAACCCTACAAGTTGAGGCTGAACAAGTTCGCAGATATGAGCAATTATGAGTTTGTGAACTTATATGCTCGCTCCAATATTAGCGACTACAGGAAGTTATATGGCAGCAGAAGAGGAGGTGCTGGTGGATTCATGTATGAGCAGGTTACAGACCTTCCTCCATCCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGGAACAAGGCGTTTGTGGTATAGTTTCCAATTCTGGATATCGACCTTTTCTTCATTTATAAAATGGTTGATTACTATAAACAAAATTTGATAGTATTGAGAGTTCTCTCTATTAGATTATGAAAATTTGAAAAATTAGGAGGTCAATGAAAAGCATTCTGCGATTAAACTTGTATAATGAATTTTTGAAATCACAAGTAATTCAATGATTTTGAAAAATGTTGCAGGTAGCTGTTGGGCGTTTTCAACTGTGGCTGCAGTTGAAGGAATCAACCAAATCAAAACCGGCCAGCTATTGTCTCTTTCAGAGCAGGAGCTGCTGGACTGCAATTTAAGGAACAGAGGTTGTAACGGAGGATTCATGGAGATCGCTTTTGATTTCATAAGTCGAAATGGTGGAGTTACCACGGAGAACAACTATCCCTACCATGCTCAAAGAGGATTCTGTCGCCTATCGAGAGTCAGTTTGATAATTCATCTAACTTCGTCAAGCAATTCAAAATTAAATCATAGTTCATCCTTTTGGAAATTATCAAACTAAGCATATATTTGTTGGATTTAACAGAGGAATTTACCGATAGTAACCATTGATGGATACGAAACCGTACCTGAAAACGAGAATGCTCTGGCGCAAGCCGTCGCAAACCAACCGGTCTCAGTCGCCATAGACGCCGCTGGAAGAGATTTCCAATTCTACTCGGAGGCAAGTTCCTAGTTCACTCGAGCAATCGATTGCTTCATCATAACTTAAAATCATACTTGCTATGCCTACAAAAACTTCATCCTTTGTAATCGAAATCCGTGGCTAAATTGCAGGGAGTGTTCGATGGGGAATGTGGAACACAGCTTAATCACGGAGTAGCGGCCATCGGATATGGAACAACCGAGGACGGAACAGACTTCTGGATCGTGAGGAATTCATGGGGAGTCGGATGGGGAGAGGAAGGTTACGTAAGGATGAAACGAGGAGTGGAGGAGCCAGAAGGTCTGTGTGGTATAACCATGGAAGCCTCCTATCCCGTCAAG

mRNA sequence

ATGGCCATTGTCAAATTTCTCATGGTTCCTCTTCTTCTGTTTGTTCTAGTTTCAGGATTGGCCGAGAGCTTTGAATTCAATGAGACGGAATTAGCATCAGAACAGAGTCTATGGAAGCTGTATGAGAGGTGGAGTAACCACCATACAATCTCAAGGGGCCTAAAGGAGAAGCACAGACGTTTCAGTGTGTTCAAAGAGAATGTGAACCATGTGTTCACAGCGAACCAAATGGAAAAACCCTACAAGTTGAGGCTGAACAAGTTCGCAGATATGAGCAATTATGAGTTTGTGAACTTATATGCTCGCTCCAATATTAGCGACTACAGGAAGTTATATGGCAGCAGAAGAGGAGGTGCTGGTGGATTCATGTATGAGCAGGTTACAGACCTTCCTCCATCCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGGAACAAGGCGTTTGTGGTAGCTGTTGGGCGTTTTCAACTGTGGCTGCAGTTGAAGGAATCAACCAAATCAAAACCGGCCAGCTATTGTCTCTTTCAGAGCAGGAGCTGCTGGACTGCAATTTAAGGAACAGAGGTTGTAACGGAGGATTCATGGAGATCGCTTTTGATTTCATAAGTCGAAATGGTGGAGTTACCACGGAGAACAACTATCCCTACCATGCTCAAAGAGGATTCTGTCGCCTATCGAGAAGGAATTTACCGATAGTAACCATTGATGGATACGAAACCGTACCTGAAAACGAGAATGCTCTGGCGCAAGCCGTCGCAAACCAACCGGTCTCAGTCGCCATAGACGCCGCTGGAAGAGATTTCCAATTCTACTCGGAGGCAATGTTCGATGGGGAATGTGGAACACAGCTTAATCACGGAGTAGCGGCCATCGGATATGGAACAACCGAGGACGGAACAGACTTCTGGATCGTGAGGAATTCATGGGGAGTCGGATGGGGAGAGGAAGGTTACGTAAGGATGAAACGAGGAGTGGAGGAGCCAGAAGGTCTGTGTGGTATAACCATGGAAGCCTCCTATCCCGTCAAG

Coding sequence (CDS)

ATGGCCATTGTCAAATTTCTCATGGTTCCTCTTCTTCTGTTTGTTCTAGTTTCAGGATTGGCCGAGAGCTTTGAATTCAATGAGACGGAATTAGCATCAGAACAGAGTCTATGGAAGCTGTATGAGAGGTGGAGTAACCACCATACAATCTCAAGGGGCCTAAAGGAGAAGCACAGACGTTTCAGTGTGTTCAAAGAGAATGTGAACCATGTGTTCACAGCGAACCAAATGGAAAAACCCTACAAGTTGAGGCTGAACAAGTTCGCAGATATGAGCAATTATGAGTTTGTGAACTTATATGCTCGCTCCAATATTAGCGACTACAGGAAGTTATATGGCAGCAGAAGAGGAGGTGCTGGTGGATTCATGTATGAGCAGGTTACAGACCTTCCTCCATCCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGGAACAAGGCGTTTGTGGTAGCTGTTGGGCGTTTTCAACTGTGGCTGCAGTTGAAGGAATCAACCAAATCAAAACCGGCCAGCTATTGTCTCTTTCAGAGCAGGAGCTGCTGGACTGCAATTTAAGGAACAGAGGTTGTAACGGAGGATTCATGGAGATCGCTTTTGATTTCATAAGTCGAAATGGTGGAGTTACCACGGAGAACAACTATCCCTACCATGCTCAAAGAGGATTCTGTCGCCTATCGAGAAGGAATTTACCGATAGTAACCATTGATGGATACGAAACCGTACCTGAAAACGAGAATGCTCTGGCGCAAGCCGTCGCAAACCAACCGGTCTCAGTCGCCATAGACGCCGCTGGAAGAGATTTCCAATTCTACTCGGAGGCAATGTTCGATGGGGAATGTGGAACACAGCTTAATCACGGAGTAGCGGCCATCGGATATGGAACAACCGAGGACGGAACAGACTTCTGGATCGTGAGGAATTCATGGGGAGTCGGATGGGGAGAGGAAGGTTACGTAAGGATGAAACGAGGAGTGGAGGAGCCAGAAGGTCTGTGTGGTATAACCATGGAAGCCTCCTATCCCGTCAAG

Protein sequence

MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRRFSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAGGFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGYETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTEDGTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK

Homology

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BLAST of MS000933 vs. NCBI nr
Match: XP_008444390.1 (PREDICTED: vignain-like [Cucumis melo])

HSP 1 Score: 601.3 bits (1549), Expect = 5.3e-168
Identity = 287/345 (83.19%), Postives = 312/345 (90.43%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI KFL+VPLLL VLVSGLAESFEF+E ELA+E+SLW+LYERW NHHTISR LKEKH+R
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           FSVFKENVNHVFT NQM KPYKL+LNKFADMSNYEFVN YARSNIS +RKL+G RR GAG
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRR-GAG 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYEQ TDLP S+DWRERGAVN IKEQG CGSCWAFS+VAAVE IN+IKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN+GCNGGFMEIAFDFI RNGG+ TEN+YPYH  RG CR SR + PIV IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 300
           E+VPENE+AL QAVANQPVSVAIDAAGRDFQFY + +FDG CGT+LNHGV AIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GTD+WIVRNSWGVGWGE+GYVRMKRGVE+PEGLCGI MEASYP+K
Sbjct: 301 GTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIK 344

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BLAST of MS000933 vs. NCBI nr
Match: XP_038885064.1 (LOW QUALITY PROTEIN: vignain-like [Benincasa hispida])

HSP 1 Score: 599.7 bits (1545), Expect = 1.5e-167
Identity = 282/345 (81.74%), Postives = 308/345 (89.28%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI+KFL++PLLL VL+SG AESFEF+E ELA+E+SLWKLYERW NHHTISR LKEKH+R
Sbjct: 29  MAIIKFLLLPLLLIVLISGFAESFEFDEKELATEESLWKLYERWGNHHTISRDLKEKHKR 88

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           F VFKENVNHVFT NQM KPYKL+LNKFADMSNYEFVN YARSNIS YRKL+G RR G  
Sbjct: 89  FKVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHGRRREGTS 148

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYE+ TDLP  IDWRERGAVN IKEQG CGSCWAFS+VAAVEGIN+I+T QLLSLSEQ
Sbjct: 149 GFMYEEATDLPSYIDWRERGAVNGIKEQGRCGSCWAFSSVAAVEGINKIRTNQLLSLSEQ 208

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN GCNGGFMEIAFDFI RNGG+ TEN+YPYH  RG CR SR + PIV IDGY
Sbjct: 209 ELLDCNYRNEGCNGGFMEIAFDFIRRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 268

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 300
           E++PENE+AL QAVANQPVSVAIDAAGRDFQFY + +FDG CGT+LNHGV AIGYGTTED
Sbjct: 269 ESIPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 328

Query: 301 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GTD+WIVRNSWGVGWGEEGYVRMKRGVE+PEGLCGI MEASYP+K
Sbjct: 329 GTDYWIVRNSWGVGWGEEGYVRMKRGVEQPEGLCGIAMEASYPIK 373

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BLAST of MS000933 vs. NCBI nr
Match: XP_004142960.1 (vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis sativus])

HSP 1 Score: 598.2 bits (1541), Expect = 4.5e-167
Identity = 285/345 (82.61%), Postives = 312/345 (90.43%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI KFL+VPLLL VLVSGLAESFEF+E ELA+E+SLW+LYERW  HHTISR LKEKH+R
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           FSVFKENVNHVFT NQM+KPYKL+LNKFADMSNYEFVN YARSNIS YRKL+  RR GAG
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLH-ERRRGAG 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYEQ TDLP S+DWRERGAVN +KEQG CGSCWAFS+VAAVEGIN+IKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN+GCNGGFMEIAFDFI RNGG+ TEN+YPYH  RG CR SR + PIV IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 300
           E+VPENE+AL QAVANQPVSVAIDAAGRDFQFYS+ +FDG CGT+LNHGV AIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GTD+W+VRNSWGVGWGE+GYVRMKRGVE+ EGLCGI MEASYP+K
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIK 344

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BLAST of MS000933 vs. NCBI nr
Match: XP_023545499.1 (vignain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.1e-162
Identity = 284/374 (75.94%), Postives = 310/374 (82.89%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI KFL+VP LL VLVSGL ESFEF+E ELA+E+SLW+LYERW NHHTISRGLKEKH+R
Sbjct: 28  MAISKFLLVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 87

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           F+VFKENVNHVFT NQM KPYKL+LNKFADMSN EFV+ YARSNIS YRKL+G R   +G
Sbjct: 88  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR---SG 147

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYEQ TDLP  IDWRERGAVNDIKEQG CGSCWAFS+VAAVEGINQIKT QLLSLSEQ
Sbjct: 148 GFMYEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSSVAAVEGINQIKTNQLLSLSEQ 207

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN+GCNGGFMEIAFDFI +NGG+ TENNYPYH  RG CR SR++ P VTIDGY
Sbjct: 208 ELLDCNFRNKGCNGGFMEIAFDFIKKNGGIATENNYPYHGARGLCRSSRKSSPTVTIDGY 267

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEA------------------------ 300
           E+VPENENAL QAVANQPVSVAIDA GRDFQFYS+A                        
Sbjct: 268 ESVPENENALMQAVANQPVSVAIDAVGRDFQFYSQASLWLIRAIDCFTIISSGIVNLKTV 327

Query: 301 -----MFDGECGTQLNHGVAAIGYGTTEDGTDFWIVRNSWGVGWGEEGYVRMKRGVEEPE 346
                +FDG CGT+LNHGV AIGYGTTE+GTD+W+VRNSWGVGWGEEGYVRMKRGVE+ E
Sbjct: 328 GKLQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEQSE 387

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BLAST of MS000933 vs. NCBI nr
Match: XP_022996765.1 (vignain-like [Cucurbita maxima])

HSP 1 Score: 582.8 bits (1501), Expect = 2.0e-162
Identity = 285/375 (76.00%), Postives = 309/375 (82.40%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI KF++VP LL VLVSGL ESFEF+E ELA+E+SLW+LYERW NHHTISRGLKEKH+R
Sbjct: 1   MAISKFILVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           F+VFKENVNHVFT NQM KPYKL+LNKFADMSN EFV+ YARSNIS YRKL+G R   +G
Sbjct: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR---SG 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYEQ TDLP  IDWRERGAVNDIKEQG CGSCWAFSTVAAVEGINQIKT QLLSLSEQ
Sbjct: 121 GFMYEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSTVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN+GCNGGFMEIAFDFI RNGG+ TENNYPYH  RG CR SR + P VTIDGY
Sbjct: 181 ELLDCNFRNKGCNGGFMEIAFDFIKRNGGIATENNYPYHGARGLCRSSRISSPTVTIDGY 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEA------------------------ 300
           E+VPENENAL QAVANQPVSVAIDA GRDFQFYS+A                        
Sbjct: 241 ESVPENENALMQAVANQPVSVAIDAVGRDFQFYSQASLGFIREIDWLTIIISSGNVNLKS 300

Query: 301 ------MFDGECGTQLNHGVAAIGYGTTEDGTDFWIVRNSWGVGWGEEGYVRMKRGVEEP 346
                 +FDG CGT+LNHGV AIGYGTTE+GTD+W+VRNSWGVGWGEEGYVRMKRGVE+ 
Sbjct: 301 VGKVQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEKA 360

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BLAST of MS000933 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.3e-128
Identity = 216/343 (62.97%), Postives = 270/343 (78.72%), Query Frame = 0


Query: 5   KFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRRFSVF 64
           KF+++ L L  LV  + ESF+F+E EL SE+SLW LYERW +HHT+SR L EK +RF+VF
Sbjct: 3   KFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFNVF 62

Query: 65  KENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAGGFMY 124
           K N  HV  AN+M+KPYKL+LNKFADM+N+EF N Y+ S +  +R   G  RG  G FMY
Sbjct: 63  KHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGN-GTFMY 122

Query: 125 EQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQELLD 184
           E+V  +P S+DWR++GAV  +K+QG CGSCWAFST+ AVEGINQIKT +L+SLSEQEL+D
Sbjct: 123 EKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVD 182

Query: 185 CNL-RNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGYETV 244
           C+  +N+GCNGG M+ AF+FI + GG+TTE NYPY A  G C +S+ N P V+IDG+E V
Sbjct: 183 CDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHENV 242

Query: 245 PEN-ENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTEDGT 304
           PEN ENAL +AVANQPVSVAIDA G DFQFYSE +F G CGT+L+HGVA +GYGTT DGT
Sbjct: 243 PENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGSCGTELDHGVAIVGYGTTIDGT 302

Query: 305 DFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
            +W V+NSWG  WGE+GY+RM+RG+ + EGLCGI MEASYP+K
Sbjct: 303 KYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

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BLAST of MS000933 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 4.7e-127
Identity = 220/347 (63.40%), Postives = 274/347 (78.96%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MA+ K L V L L  LV G+A SF+F+E +L SE+SLW LYERW +HHT+SR L EKH+R
Sbjct: 1   MAMKKLLWVVLSL-SLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           F+VFK NV HV   N+M+KPYKL+LNKFADM+N+EF + YA S ++ ++   GS+  G+G
Sbjct: 61  FNVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQH-GSG 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
            FMYE+V  +P S+DWR++GAV D+K+QG CGSCWAFST+ AVEGINQIKT +L+SLSEQ
Sbjct: 121 TFMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQ 180

Query: 181 ELLDCNL-RNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDG 240
           EL+DC+   N+GCNGG ME AF+FI + GG+TTE+NYPY AQ G C  S+ N   V+IDG
Sbjct: 181 ELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDG 240

Query: 241 YETVPEN-ENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTT 300
           +E VP N ENAL +AVANQPVSVAIDA G DFQFYSE +F G+C T LNHGVA +GYGTT
Sbjct: 241 HENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTT 300

Query: 301 EDGTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
            DGT++WIVRNSWG  WGE+GY+RM+R + + EGLCGI M ASYP+K
Sbjct: 301 VDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

[Close]

BLAST of MS000933 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 449.5 bits (1155), Expect = 3.4e-125
Identity = 217/347 (62.54%), Postives = 270/347 (77.81%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MA  K L V +L F LV G+A SF+F++ +LASE+SLW LYERW +HHT+SR L EKH+R
Sbjct: 1   MATKKLLWV-VLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           F+VFK N+ HV   N+M+KPYKL+LNKFADM+N+EF + YA S ++  R   G+     G
Sbjct: 61  FNVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHEN-G 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
            FMYE+V  +PPS+DWR++GAV D+K+QG CGSCWAFSTV AVEGINQIKT +L++LSEQ
Sbjct: 121 AFMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQ 180

Query: 181 ELLDCNL-RNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDG 240
           EL+DC+   N+GCNGG ME AF+FI + GG+TTE+NYPY AQ G C  S+ N   V+IDG
Sbjct: 181 ELVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDG 240

Query: 241 YETVPEN-ENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTT 300
           +E VP N E+AL +AVANQPVSVAIDA G DFQFYSE +F G+C T LNHGVA +GYGTT
Sbjct: 241 HENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTT 300

Query: 301 EDGTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
            DGT++WIVRNSWG  WGE GY+RM+R + + EGLCGI M  SYP+K
Sbjct: 301 VDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

[Close]

BLAST of MS000933 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 7.1e-115
Identity = 196/344 (56.98%), Postives = 253/344 (73.55%), Query Frame = 0


Query: 4   VKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRRFSV 63
           +K  +V  L  ++V    +  +F+  ++ SE SLW+LYERW +HHT++R L+EK +RF+V
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 64  FKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAGGFM 123
           FK NV H+   N+ +K YKL+LNKF DM++ EF   YA SNI  +R   G ++     FM
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKK-ATKSFM 120

Query: 124 YEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQELL 183
           Y  V  LP S+DWR+ GAV  +K QG CGSCWAFSTV AVEGINQI+T +L SLSEQEL+
Sbjct: 121 YANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELV 180

Query: 184 DCNL-RNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGYET 243
           DC+  +N+GCNGG M++AF+FI   GG+T+E  YPY A    C  ++ N P+V+IDG+E 
Sbjct: 181 DCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHED 240

Query: 244 VPEN-ENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTEDG 303
           VP+N E+ L +AVANQPVSVAIDA G DFQFYSE +F G CGT+LNHGVA +GYGTT DG
Sbjct: 241 VPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDG 300

Query: 304 TDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           T +WIV+NSWG  WGE+GY+RM+RG+   EGLCGI MEASYP+K
Sbjct: 301 TKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

[Close]

BLAST of MS000933 vs. ExPASy Swiss-Prot
Match: P43156 (Thiol protease SEN102 OS=Hemerocallis sp. OX=29711 GN=SEN102 PE=2 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 4.6e-114
Identity = 204/349 (58.45%), Postives = 258/349 (73.93%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MA  KF+ + L+    +S +A+S  F E +LASE SLW LYE+W  HHT++R L EK+RR
Sbjct: 1   MAKPKFIALALVALSFLS-IAQSIPFTEKDLASEDSLWNLYEKWRTHHTVARDLDEKNRR 60

Query: 61  FSVFKENVNHVFTANQ-MEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGA 120
           F+VFKENV  +   NQ  + PYKL LNKF DM+N EF + YA S I  +R   G ++   
Sbjct: 61  FNVFKENVKFIHEFNQKKDAPYKLALNKFGDMTNQEFRSKYAGSKIQHHRSQRGIQK-NT 120

Query: 121 GGFMYEQVTDLP-PSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLS 180
           G FMYE V  LP  SIDWR +GAV  +K+QG CGSCWAFST+A+VEGINQIKTG+L+SLS
Sbjct: 121 GSFMYENVGSLPAASIDWRAKGAVTGVKDQGQCGSCWAFSTIASVEGINQIKTGELVSLS 180

Query: 181 EQELLDCNLR-NRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTI 240
           EQEL+DC+   N GCNGG M+ AF+FI +N G+TTE++YPY  Q G C  +  N P+V+I
Sbjct: 181 EQELVDCDTSYNEGCNGGLMDYAFEFIQKN-GITTEDSYPYAEQDGTCASNLLNSPVVSI 240

Query: 241 DGYETVP-ENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYG 300
           DG++ VP  NENAL QAVANQP+SV+I+A+G  FQFYSE +F G CGT+L+HGVA +GYG
Sbjct: 241 DGHQDVPANNENALMQAVANQPISVSIEASGYGFQFYSEGVFTGRCGTELDHGVAIVGYG 300

Query: 301 TTEDGTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
            T DGT +WIV+NSWG  WGE GY+RM+RG+ +  G CGI MEASYP+K
Sbjct: 301 ATRDGTKYWIVKNSWGEEWGESGYIRMQRGISDKRGKCGIAMEASYPIK 346

[Close]

BLAST of MS000933 vs. ExPASy TrEMBL
Match: A0A1S3BA70 (vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.6e-168
Identity = 287/345 (83.19%), Postives = 312/345 (90.43%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI KFL+VPLLL VLVSGLAESFEF+E ELA+E+SLW+LYERW NHHTISR LKEKH+R
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           FSVFKENVNHVFT NQM KPYKL+LNKFADMSNYEFVN YARSNIS +RKL+G RR GAG
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRR-GAG 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYEQ TDLP S+DWRERGAVN IKEQG CGSCWAFS+VAAVE IN+IKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN+GCNGGFMEIAFDFI RNGG+ TEN+YPYH  RG CR SR + PIV IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 300
           E+VPENE+AL QAVANQPVSVAIDAAGRDFQFY + +FDG CGT+LNHGV AIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GTD+WIVRNSWGVGWGE+GYVRMKRGVE+PEGLCGI MEASYP+K
Sbjct: 301 GTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIK 344

[Close]

BLAST of MS000933 vs. ExPASy TrEMBL
Match: A0A0A0LMU4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.2e-167
Identity = 285/345 (82.61%), Postives = 312/345 (90.43%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI KFL+VPLLL VLVSGLAESFEF+E ELA+E+SLW+LYERW  HHTISR LKEKH+R
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           FSVFKENVNHVFT NQM+KPYKL+LNKFADMSNYEFVN YARSNIS YRKL+  RR GAG
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLH-ERRRGAG 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYEQ TDLP S+DWRERGAVN +KEQG CGSCWAFS+VAAVEGIN+IKT QLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN+GCNGGFMEIAFDFI RNGG+ TEN+YPYH  RG CR SR + PIV IDGY
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 300
           E+VPENE+AL QAVANQPVSVAIDAAGRDFQFYS+ +FDG CGT+LNHGV AIGYGTTED
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GTD+W+VRNSWGVGWGE+GYVRMKRGVE+ EGLCGI MEASYP+K
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIK 344

[Close]

BLAST of MS000933 vs. ExPASy TrEMBL
Match: A0A6J1K7P4 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111491900 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.5e-163
Identity = 285/375 (76.00%), Postives = 309/375 (82.40%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI KF++VP LL VLVSGL ESFEF+E ELA+E+SLW+LYERW NHHTISRGLKEKH+R
Sbjct: 1   MAISKFILVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           F+VFKENVNHVFT NQM KPYKL+LNKFADMSN EFV+ YARSNIS YRKL+G R   +G
Sbjct: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR---SG 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYEQ TDLP  IDWRERGAVNDIKEQG CGSCWAFSTVAAVEGINQIKT QLLSLSEQ
Sbjct: 121 GFMYEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSTVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RN+GCNGGFMEIAFDFI RNGG+ TENNYPYH  RG CR SR + P VTIDGY
Sbjct: 181 ELLDCNFRNKGCNGGFMEIAFDFIKRNGGIATENNYPYHGARGLCRSSRISSPTVTIDGY 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEA------------------------ 300
           E+VPENENAL QAVANQPVSVAIDA GRDFQFYS+A                        
Sbjct: 241 ESVPENENALMQAVANQPVSVAIDAVGRDFQFYSQASLGFIREIDWLTIIISSGNVNLKS 300

Query: 301 ------MFDGECGTQLNHGVAAIGYGTTEDGTDFWIVRNSWGVGWGEEGYVRMKRGVEEP 346
                 +FDG CGT+LNHGV AIGYGTTE+GTD+W+VRNSWGVGWGEEGYVRMKRGVE+ 
Sbjct: 301 VGKVQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEKA 360

[Close]

BLAST of MS000933 vs. ExPASy TrEMBL
Match: A0A6J1GHN5 (vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 5.0e-156
Identity = 264/345 (76.52%), Postives = 301/345 (87.25%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           MAI K L+VP L+ VL+SGLA+SFEF+E ELA++ SLWKLYERWS+HH ISR LKEKH+R
Sbjct: 1   MAISKCLLVPFLI-VLISGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           ++VFKEN NHV T NQM KPYKL+LNKFADMSNYEFVN+YARSNI+ YR+L+G RR GA 
Sbjct: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNVYARSNITHYRRLHGKRREGAS 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYE+ TDLP  IDWRERGAV+DIK+QG CGSCWAFS VAAVEGINQIKT QLLSLSEQ
Sbjct: 121 GFMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RNRGC GGFME A++FI RNGG+ +ENNYPY   RG CR SR   PIVTIDG+
Sbjct: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 300
           E+VPENENAL QAVANQPVSV+I+A GRDFQFY + +FDG+CGT+LNHGV  IGYGTT+ 
Sbjct: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGDCGTELNHGVVVIGYGTTDG 300

Query: 301 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GTD+W VRNSWGVGWGE+GY+RMKRGVE+PEGLCGI MEASYP+K
Sbjct: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLK 344

[Close]

BLAST of MS000933 vs. ExPASy TrEMBL
Match: A0A6J1KIL0 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.9e-155
Identity = 266/345 (77.10%), Postives = 298/345 (86.38%), Query Frame = 0


Query: 1   MAIVKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRR 60
           M I K L+VP L+ VLVSGLA+SFEF+E ELA++ SLWKLYERWS+HH ISR LKEKH+R
Sbjct: 1   MPISKCLLVPFLI-VLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60

Query: 61  FSVFKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAG 120
           ++VFKEN NHV T NQM KPYKL+LNKFADMSNYEFVNLYARSNI+ YR+L+G RR GA 
Sbjct: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120

Query: 121 GFMYEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQ 180
           GFMYE+ TDLP  IDWRERGAVNDIK QG CGSCWAFS VAAVEGINQIKT QLLSLSEQ
Sbjct: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNLRNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 240
           ELLDCN RNRGC GGFME A++FI RNGG+ +ENNYPY   RG CR SR   PIVTIDG+
Sbjct: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240

Query: 241 ETVPENENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 300
           E+VPENENAL QAVANQPVSV+I+A GRDFQFY + +FDG CGT+LNHGV  IGYGTT+ 
Sbjct: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300

Query: 301 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GTD+W VRNSWGVGWGE+GY+RMKRGVE+PEGLCGI MEASYP+K
Sbjct: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 344

[Close]

BLAST of MS000933 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 415.2 bits (1066), Expect = 5.0e-116
Identity = 196/344 (56.98%), Postives = 253/344 (73.55%), Query Frame = 0


Query: 4   VKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRRFSV 63
           +K  +V  L  ++V    +  +F+  ++ SE SLW+LYERW +HHT++R L+EK +RF+V
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 64  FKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAGGFM 123
           FK NV H+   N+ +K YKL+LNKF DM++ EF   YA SNI  +R   G ++     FM
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKK-ATKSFM 120

Query: 124 YEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQELL 183
           Y  V  LP S+DWR+ GAV  +K QG CGSCWAFSTV AVEGINQI+T +L SLSEQEL+
Sbjct: 121 YANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELV 180

Query: 184 DCNL-RNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGYET 243
           DC+  +N+GCNGG M++AF+FI   GG+T+E  YPY A    C  ++ N P+V+IDG+E 
Sbjct: 181 DCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHED 240

Query: 244 VPEN-ENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTEDG 303
           VP+N E+ L +AVANQPVSVAIDA G DFQFYSE +F G CGT+LNHGVA +GYGTT DG
Sbjct: 241 VPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDG 300

Query: 304 TDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           T +WIV+NSWG  WGE+GY+RM+RG+   EGLCGI MEASYP+K
Sbjct: 301 TKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

[Close]

BLAST of MS000933 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 411.4 bits (1056), Expect = 7.3e-115
Identity = 200/346 (57.80%), Postives = 261/346 (75.43%), Query Frame = 0


Query: 4   VKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRRFSV 63
           +K L++  L  +++   A  F++++ E+ SE+ L  LY+RW +HH++ R L E+ +RF+V
Sbjct: 1   MKKLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNV 60

Query: 64  FKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAGGFM 123
           F+ NV HV   N+  + YKL+LNKFAD++  EF N Y  SNI  +R L G +R G+  FM
Sbjct: 61  FRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKR-GSKQFM 120

Query: 124 Y--EQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQE 183
           Y  E ++ LP S+DWR++GAV +IK QG CGSCWAFSTVAAVEGIN+IKT +L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 184 LLDCNLR-NRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGY 243
           L+DC+ + N GCNGG MEIAF+FI +NGG+TTE++YPY    G C  S+ N  +VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 244 ETVPEN-ENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTE 303
           E VPEN ENAL +AVANQPVSVAIDA   DFQFYSE +F G CGT+LNHGVAA+GYG +E
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SE 300

Query: 304 DGTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
            G  +WIVRNSWG  WGE GY++++R ++EPEG CGI MEASYP+K
Sbjct: 301 RGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

[Close]

BLAST of MS000933 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 411.0 bits (1055), Expect = 9.5e-115
Identity = 198/345 (57.39%), Postives = 256/345 (74.20%), Query Frame = 0


Query: 4   VKFLMVPLLLFVLVSGLAESFEFNETELASEQSLWKLYERWSNHHTISRGLKEKHRRFSV 63
           +K   + L+ F+ +   ++ F+F+E EL +E+++WKLYERW  HH++SR   E  +RF+V
Sbjct: 1   MKLFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNV 60

Query: 64  FKENVNHVFTANQMEKPYKLRLNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAGGFM 123
           F+ NV HV   N+  KPYKL++N+FAD++++EF + YA SN+  +R L G +R G+GGFM
Sbjct: 61  FRHNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKR-GSGGFM 120

Query: 124 YEQVTDLPPSIDWRERGAVNDIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQELL 183
           YE VT +P S+DWRE+GAV ++K Q  CGSCWAFSTVAAVEGIN+I+T +L+SLSEQEL+
Sbjct: 121 YENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELV 180

Query: 184 DCNL-RNRGCNGGFMEIAFDFISRNGGVTTENNYPYHAQR-GFCRLSRRNLPIVTIDGYE 243
           DC+   N+GC GG ME AF+FI  NGG+ TE  YPY +    FCR +      VTIDG+E
Sbjct: 181 DCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHE 240

Query: 244 TVPEN-ENALAQAVANQPVSVAIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTED 303
            VPEN E  L +AVA+QPVSVAIDA   DFQ YSE +F GECGTQLNHGV  +GYG T++
Sbjct: 241 HVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKN 300

Query: 304 GTDFWIVRNSWGVGWGEEGYVRMKRGVEEPEGLCGITMEASYPVK 346
           GT +WIVRNSWG  WGE GYVR++RG+ E EG CGI MEASYP K
Sbjct: 301 GTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

[Close]

BLAST of MS000933 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 333.2 bits (853), Expect = 2.5e-91
Identity = 163/323 (50.46%), Postives = 223/323 (69.04%), Query Frame = 0


Query: 26  FNETELASEQSLWKLYERW-SNHHTISRGLKEKHRRFSVFKENVNHVFTANQMEKPYKLR 85
           +    L +   L +L+E W S H    + ++EK  RF VF+EN+ H+   N     Y L 
Sbjct: 36  YTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEINSYWLG 95

Query: 86  LNKFADMSNYEFVNLYARSNISDYRKLYGSRRGGAGGFMYEQVTDLPPSIDWRERGAVND 145
           LN+FAD+++ EF   Y    +   +  +  +R  +  F Y  +TDLP S+DWR++GAV  
Sbjct: 96  LNEFADLTHEEFKGRY----LGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAVAP 155

Query: 146 IKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQELLDCNLR-NRGCNGGFMEIAFDF 205
           +K+QG CGSCWAFSTVAAVEGINQI TG L SLSEQEL+DC+   N GCNGG M+ AF +
Sbjct: 156 VKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTFNSGCNGGLMDYAFQY 215

Query: 206 ISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGYETVPENEN-ALAQAVANQPVSVA 265
           I   GG+  E++YPY  + G C+  + ++  VTI GYE VPEN++ +L +A+A+QPVSVA
Sbjct: 216 IISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPVSVA 275

Query: 266 IDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTEDGTDFWIVRNSWGVGWGEEGYVR 325
           I+A+GRDFQFY   +F+G+CGT L+HGVAA+GYG+++ G+D+ IV+NSWG  WGE+G++R
Sbjct: 276 IEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGYGSSK-GSDYVIVKNSWGPRWGEKGFIR 335

Query: 326 MKRGVEEPEGLCGITMEASYPVK 346
           MKR   +PEGLCGI   ASYP K
Sbjct: 336 MKRNTGKPEGLCGINKMASYPTK 353

[Close]

BLAST of MS000933 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.3e-91
Identity = 167/324 (51.54%), Postives = 219/324 (67.59%), Query Frame = 0


Query: 33  SEQSLWKLYERWSNHHTISR----GL-KEKHRRFSVFKENVNHVFTANQMEKPYKLRLNK 92
           S+  + ++YE W   H   +    GL  EK +RF +FK+N+  +   N     YKL L +
Sbjct: 42  SDSEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTR 101

Query: 93  FADMSNYEFVNLY----ARSNISDYRKLYGSRRGGAGGFMYEQVTDLPPSIDWRERGAVN 152
           FAD++N E+ ++Y        +      Y +R G A          LP S+DWR+ GAV 
Sbjct: 102 FADLTNEEYRSMYLGAKPTKRVLKTSDRYQARVGDA----------LPDSVDWRKEGAVA 161

Query: 153 DIKEQGVCGSCWAFSTVAAVEGINQIKTGQLLSLSEQELLDCNLR-NRGCNGGFMEIAFD 212
           D+K+QG CGSCWAFST+ AVEGIN+I TG L+SLSEQEL+DC+   N+GCNGG M+ AF+
Sbjct: 162 DVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFE 221

Query: 213 FISRNGGVTTENNYPYHAQRGFCRLSRRNLPIVTIDGYETVPEN-ENALAQAVANQPVSV 272
           FI +NGG+ TE +YPY A  G C  +R+N  +VTID YE VPEN E +L +A+A+QP+SV
Sbjct: 222 FIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISV 281

Query: 273 AIDAAGRDFQFYSEAMFDGECGTQLNHGVAAIGYGTTEDGTDFWIVRNSWGVGWGEEGYV 332
           AI+A GR FQ YS  +FDG CGT+L+HGV A+GYG TE+G D+WIVRNSWG  WGE GY+
Sbjct: 282 AIEAGGRAFQLYSSGVFDGLCGTELDHGVVAVGYG-TENGKDYWIVRNSWGNRWGESGYI 341

Query: 333 RMKRGVEEPEGLCGITMEASYPVK 346
           +M R +E P G CGI MEASYP+K
Sbjct: 342 KMARNIEAPTGKCGIAMEASYPIK 354

The following BLAST results are available for this feature:

BLAST of MS000933 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008444390.1	5.3e-168	83.19	PREDICTED: vignain-like [Cucumis melo]	[more]
XP_038885064.1	1.5e-167	81.74	LOW QUALITY PROTEIN: vignain-like [Benincasa hispida]	[more]
XP_004142960.1	4.5e-167	82.61	vignain [Cucumis sativus] >KGN62334.1 hypothetical protein Csa_018585 [Cucumis s...	[more]
XP_023545499.1	1.1e-162	75.94	vignain-like [Cucurbita pepo subsp. pepo]	[more]
XP_022996765.1	2.0e-162	76.00	vignain-like [Cucurbita maxima]	[more]

BLAST of MS000933 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65039	3.3e-128	62.97	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]
P12412	4.7e-127	63.40	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
P25803	3.4e-125	62.54	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9FGR9	7.1e-115	56.98	KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ...	[more]
P43156	4.6e-114	58.45	Thiol protease SEN102 OS=Hemerocallis sp. OX=29711 GN=SEN102 PE=2 SV=1	[more]

BLAST of MS000933 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BA70	2.6e-168	83.19	vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1	[more]
A0A0A0LMU4	2.2e-167	82.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1	[more]
A0A6J1K7P4	9.5e-163	76.00	vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111491900 PE=3 SV=1	[more]
A0A6J1GHN5	5.0e-156	76.52	vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1	[more]
A0A6J1KIL0	1.9e-155	77.10	vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1	[more]

BLAST of MS000933 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50260.1	5.0e-116	56.98	Cysteine proteinases superfamily protein	[more]
AT3G48340.1	7.3e-115	57.80	Cysteine proteinases superfamily protein	[more]
AT3G48350.1	9.5e-115	57.39	Cysteine proteinases superfamily protein	[more]
AT4G35350.1	2.5e-91	50.46	xylem cysteine peptidase 1	[more]
AT5G43060.1	7.3e-91	51.54	Granulin repeat cysteine protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 304..310 score: 61.92 coord: 288..298 score: 51.0 coord: 148..163 score: 65.28
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 130..344 e-value: 3.2E-110 score: 382.2
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 130..344 e-value: 2.3E-75 score: 253.4
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 41..96 e-value: 8.1E-10 score: 48.6
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 41..96 e-value: 3.0E-10 score: 40.4
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 16..345 e-value: 2.0E-113 score: 381.4
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 21..344
None	No IPR available	PANTHER	PTHR12411:SF706	KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1	coord: 21..344
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 286..296
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 148..159
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 304..323
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 131..343 e-value: 2.33109E-88 score: 262.56
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 33..344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000933.1	MS000933.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity