MS000922 (gene) Bitter gourd (TR) v1

Overview


Name	MS000922
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	NAD(P)-linked oxidoreductase superfamily protein
Location	scaffold36: 278455 .. 279597 (+)
RNA-Seq Expression	MS000922
Synteny	MS000922

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAGCACTACTATGCCAACAGTGTCGCTCAAGTCTTCCGCGTCCGGGCACAGCGTTCGAATGCCCATACTTGGTATGGGCACGGCGGCCGACCCATTTGACGCGTCCGCCTTGAAAGCCGCAGTACCCGAGGCCATCAGGCTTGGTTACCGCCATTTCGACACCGCTGCATCATATGGCTCCGAGCAACCTCTCGGAGAAGCCATTGCAGAAGCACTAGAGCTCGGCCTCTTGAGTTCTAGGGAGGAGGTCTTCGTAACATCCAAGCTCTGGTGTGGCGATGCCCATCCTAATACAGTCCTTCCTGCTCTCCGCAACTCTCTCAGGTTACCGATTGTAATTTGGTTTGTATTAATTTTGTTATTTTCCCTCCCCCTAAAAACCAAACAAAAAATAAAGGAAAGTTCTAAAACAGAACAGAACATTGAAGCTGGAGTACCTGGACCTGTATCTCGTACACTGGCCCATCAGCCTAAAGCCAGGAAAAGACGACTTCTCCATACACAGCAGCGACCTGGTTCCAATGGACTTAGAGTCCGTTTGGGGAGGCATGGAGGAATGCCAAAGACTCGGCCTGGCCAGAGCAATTGGCATCTGCAACTTCTCTTGCAAGAAGCTCCAAAACCTTCTCTCTTTCTCCAAAATCCCTCCCGCAGTGAACCAAGTAAGAGTAAGACAATGAAAAATGAGACAAAAATGAAAAATGTAGCGACTGTTGTGTTGTTTTGGTTTGGTCAGGTGGAGATGAGCCCGGTGTGGCAGCAGAGGAAGCTGAGAGAATTCTGCAATTCGAATGGGATCGTAGTGACGGCCTACTCTCCTCTGGGTGCGAAGGGCGCGTCGTGGGGGACCAGCCAGGTGATGGACTGCGAAATTCTCAAGGACATTGCGGAGGCCACGCACAGGACGGTGGCGCAGGTATCTCTGAGATGGGTGTACGAACAGGGGGTGACCCTGCTGGTGAAGAGCACCAACAAGAGGAGAATGGAAGAGAATCTGTCCATTTTTGATTGGGCGTTGACGGAGGAGCATCACCGCAGCATAAATCAGATCAAGCAGCAGAGAGGGCTGTCTAGGCACGATTTCATCTCCGCCCATGGGCCCTACAAATCTATTCAACAACTATGGGACGGGGAGTTG

mRNA sequence

ATGATGAGCACTACTATGCCAACAGTGTCGCTCAAGTCTTCCGCGTCCGGGCACAGCGTTCGAATGCCCATACTTGGTATGGGCACGGCGGCCGACCCATTTGACGCGTCCGCCTTGAAAGCCGCAGTACCCGAGGCCATCAGGCTTGGTTACCGCCATTTCGACACCGCTGCATCATATGGCTCCGAGCAACCTCTCGGAGAAGCCATTGCAGAAGCACTAGAGCTCGGCCTCTTGAGTTCTAGGGAGGAGGTCTTCGTAACATCCAAGCTCTGGTGTGGCGATGCCCATCCTAATACAGTCCTTCCTGCTCTCCGCAACTCTCTCAGAACATTGAAGCTGGAGTACCTGGACCTGTATCTCGTACACTGGCCCATCAGCCTAAAGCCAGGAAAAGACGACTTCTCCATACACAGCAGCGACCTGGTTCCAATGGACTTAGAGTCCGTTTGGGGAGGCATGGAGGAATGCCAAAGACTCGGCCTGGCCAGAGCAATTGGCATCTGCAACTTCTCTTGCAAGAAGCTCCAAAACCTTCTCTCTTTCTCCAAAATCCCTCCCGCAGTGAACCAAGTGGAGATGAGCCCGGTGTGGCAGCAGAGGAAGCTGAGAGAATTCTGCAATTCGAATGGGATCGTAGTGACGGCCTACTCTCCTCTGGGTGCGAAGGGCGCGTCGTGGGGGACCAGCCAGGTGATGGACTGCGAAATTCTCAAGGACATTGCGGAGGCCACGCACAGGACGGTGGCGCAGGTATCTCTGAGATGGGTGTACGAACAGGGGGTGACCCTGCTGGTGAAGAGCACCAACAAGAGGAGAATGGAAGAGAATCTGTCCATTTTTGATTGGGCGTTGACGGAGGAGCATCACCGCAGCATAAATCAGATCAAGCAGCAGAGAGGGCTGTCTAGGCACGATTTCATCTCCGCCCATGGGCCCTACAAATCTATTCAACAACTATGGGACGGGGAGTTG

Coding sequence (CDS)

ATGATGAGCACTACTATGCCAACAGTGTCGCTCAAGTCTTCCGCGTCCGGGCACAGCGTTCGAATGCCCATACTTGGTATGGGCACGGCGGCCGACCCATTTGACGCGTCCGCCTTGAAAGCCGCAGTACCCGAGGCCATCAGGCTTGGTTACCGCCATTTCGACACCGCTGCATCATATGGCTCCGAGCAACCTCTCGGAGAAGCCATTGCAGAAGCACTAGAGCTCGGCCTCTTGAGTTCTAGGGAGGAGGTCTTCGTAACATCCAAGCTCTGGTGTGGCGATGCCCATCCTAATACAGTCCTTCCTGCTCTCCGCAACTCTCTCAGAACATTGAAGCTGGAGTACCTGGACCTGTATCTCGTACACTGGCCCATCAGCCTAAAGCCAGGAAAAGACGACTTCTCCATACACAGCAGCGACCTGGTTCCAATGGACTTAGAGTCCGTTTGGGGAGGCATGGAGGAATGCCAAAGACTCGGCCTGGCCAGAGCAATTGGCATCTGCAACTTCTCTTGCAAGAAGCTCCAAAACCTTCTCTCTTTCTCCAAAATCCCTCCCGCAGTGAACCAAGTGGAGATGAGCCCGGTGTGGCAGCAGAGGAAGCTGAGAGAATTCTGCAATTCGAATGGGATCGTAGTGACGGCCTACTCTCCTCTGGGTGCGAAGGGCGCGTCGTGGGGGACCAGCCAGGTGATGGACTGCGAAATTCTCAAGGACATTGCGGAGGCCACGCACAGGACGGTGGCGCAGGTATCTCTGAGATGGGTGTACGAACAGGGGGTGACCCTGCTGGTGAAGAGCACCAACAAGAGGAGAATGGAAGAGAATCTGTCCATTTTTGATTGGGCGTTGACGGAGGAGCATCACCGCAGCATAAATCAGATCAAGCAGCAGAGAGGGCTGTCTAGGCACGATTTCATCTCCGCCCATGGGCCCTACAAATCTATTCAACAACTATGGGACGGGGAGTTG

Protein sequence

MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISAHGPYKSIQQLWDGEL

Homology

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BLAST of MS000922 vs. NCBI nr
Match: XP_022132268.1 (non-functional NADPH-dependent codeinone reductase 2-like [Momordica charantia])

HSP 1 Score: 659.4 bits (1700), Expect = 1.5e-185
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 0


Query: 1   MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASY 60
           MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASY
Sbjct: 13  MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASY 72

Query: 61  GSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLY 120
           GSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLY
Sbjct: 73  GSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLY 132

Query: 121 LVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLL 180
           LVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIG+CNFSCKKLQNLL
Sbjct: 133 LVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGVCNFSCKKLQNLL 192

Query: 181 SFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKD 240
           SFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKD
Sbjct: 193 SFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKD 252

Query: 241 IAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQR 300
           IAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQR
Sbjct: 253 IAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQR 312

Query: 301 GLSRHDFISAHGPYKSIQQLWDGEL 326
           GLSRHDFISAHGPYKSIQQLWDGEL
Sbjct: 313 GLSRHDFISAHGPYKSIQQLWDGEL 337

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BLAST of MS000922 vs. NCBI nr
Match: KAG8640091.1 (hypothetical protein MANES_13G023400v8 [Manihot esculenta])

HSP 1 Score: 441.8 bits (1135), Expect = 5.1e-120
Identity = 207/319 (64.89%), Postives = 257/319 (80.56%), Query Frame = 0


Query: 7   PTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPL 66
           P + L S A GH   MP++GMGTAADPFD + +K AV EAI++GY+HFDTA  Y SE+ L
Sbjct: 57  PKIKLASYA-GHQ-EMPVIGMGTAADPFDEATMKTAVLEAIKVGYKHFDTAPLYRSEKAL 116

Query: 67  GEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPI 126
           GEAIAEAL LGL+ SR+E+F+TSKLWC DAH + V+PAL NSLR L+LEYLDLYL+HWPI
Sbjct: 117 GEAIAEALSLGLIGSRDELFITSKLWCSDAHSDLVIPALNNSLRNLQLEYLDLYLIHWPI 176

Query: 127 SLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIP 186
           S KPG+  F I   +L+PMD +SVW  MEECQ+LGL +AIG+CNFSCKKL++LL+F+ IP
Sbjct: 177 SCKPGRSMFPIPKDELLPMDFKSVWAAMEECQQLGLTKAIGVCNFSCKKLEHLLAFATIP 236

Query: 187 PAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATH 246
           PAVNQVEMSP+WQQ+KLREFC +N IVVTAYSPLGAKGA WGTS VMD E+L +IA+   
Sbjct: 237 PAVNQVEMSPLWQQKKLREFCKANDIVVTAYSPLGAKGARWGTSFVMDNEVLNEIAKTRG 296

Query: 247 RTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHD 306
           +TVAQV LRWV+EQG + +VKS  K R++EN+ IFDWAL E+ H  INQI Q+R   +  
Sbjct: 297 KTVAQVCLRWVHEQGTSFIVKSYRKGRLKENMDIFDWALLEDDHERINQIPQRRLQPKEQ 356

Query: 307 FISAHGPYKSIQQLWDGEL 326
            +SA+GPYKS+++LWDGE+
Sbjct: 357 LVSANGPYKSLEELWDGEI 373

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BLAST of MS000922 vs. NCBI nr
Match: KAE8037831.1 (hypothetical protein FH972_010386 [Carpinus fangiana])

HSP 1 Score: 439.5 bits (1129), Expect = 2.5e-119
Identity = 208/317 (65.62%), Postives = 259/317 (81.70%), Query Frame = 0


Query: 9   VSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGE 68
           V L SS+ G+   M ++G G AAD  D + LK+AV EAI+LGYRHF+TA++YGSEQ LGE
Sbjct: 10  VVLSSSSFGNKA-MSVIGFGMAADSNDGATLKSAVLEAIKLGYRHFNTASAYGSEQALGE 69

Query: 69  AIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISL 128
            IA+AL+LGL+SSREE+F+TSKLW  DAHP+ VLP L  S+RTL+LEY+DLYL+HWPI++
Sbjct: 70  GIAQALKLGLVSSREELFITSKLWSSDAHPDLVLPTLHKSVRTLQLEYIDLYLIHWPITV 129

Query: 129 KPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPA 188
           KPGK  +     D++PMD +SVW  MEECQRLGLA+ IG+ NFSCKKLQNLLS S IPPA
Sbjct: 130 KPGKGGWPFAEEDMMPMDFKSVWAAMEECQRLGLAKFIGVSNFSCKKLQNLLSSSTIPPA 189

Query: 189 VNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRT 248
           VNQVEMSPVWQQ+KLR+FC  NGIVVTA+SPLGAKG SWGT+ VM+ E+L DIA+A  +T
Sbjct: 190 VNQVEMSPVWQQKKLRDFCKENGIVVTAFSPLGAKGTSWGTNHVMENEVLMDIAKARGKT 249

Query: 249 VAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFI 308
           VAQV LRW+YEQGVTL++KS NK R++ENL IFDW L+E+  + I++IKQ R + + + I
Sbjct: 250 VAQVCLRWMYEQGVTLVMKSYNKERLKENLQIFDWELSEDDSKKISEIKQHRMMLKEELI 309

Query: 309 SAHGPYKSIQQLWDGEL 326
           SA GPYKSI++LWDGEL
Sbjct: 310 SARGPYKSIEELWDGEL 325

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BLAST of MS000922 vs. NCBI nr
Match: KAF3966331.1 (hypothetical protein CMV_009552 [Castanea mollissima])

HSP 1 Score: 438.7 bits (1127), Expect = 4.3e-119
Identity = 202/315 (64.13%), Postives = 258/315 (81.90%), Query Frame = 0


Query: 11  LKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAI 70
           ++ S+S   V MP++G GTAAD  D + L +AV E+I+LGYRHFDTA+ YGSEQ LGEAI
Sbjct: 1   MELSSSSGPVSMPMIGFGTAADNNDGATLISAVLESIKLGYRHFDTASFYGSEQALGEAI 60

Query: 71  AEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKP 130
           AEAL LGL++SR+++F+TSKLW  +AH + VLP+L+ SLRTL+LEYLDLYL+HWPI++ P
Sbjct: 61  AEALRLGLIASRDDLFITSKLWPSEAHAHLVLPSLQKSLRTLQLEYLDLYLIHWPITVTP 120

Query: 131 GKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVN 190
           GK +F     DL+PMD +SVW  MEECQRLGL ++IG+ NFSCKKL+NLLSF+ IPP+VN
Sbjct: 121 GKREFPFDKQDLMPMDFKSVWAAMEECQRLGLTKSIGVSNFSCKKLENLLSFATIPPSVN 180

Query: 191 QVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVA 250
           QVEM+PVWQQ+KL EFC  NGI+VTAYSPLGAKG+SWGT+ VMD E+LK+IA+   +TVA
Sbjct: 181 QVEMNPVWQQKKLTEFCKPNGIIVTAYSPLGAKGSSWGTNAVMDNEVLKEIAKTRGKTVA 240

Query: 251 QVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISA 310
           QV LRW+YEQG   +VKS NK R++ENL IFDW L+E+ +  I QIKQ+R + R + +SA
Sbjct: 241 QVCLRWIYEQGAAPIVKSYNKERLKENLQIFDWELSEDDYDKIGQIKQRRMMPREELVSA 300

Query: 311 HGPYKSIQQLWDGEL 326
           HGPYKS+++LWDGEL
Sbjct: 301 HGPYKSVEELWDGEL 315

[Close]

BLAST of MS000922 vs. NCBI nr
Match: KAF3960187.1 (hypothetical protein CMV_015076 [Castanea mollissima])

HSP 1 Score: 438.0 bits (1125), Expect = 7.3e-119
Identity = 200/315 (63.49%), Postives = 259/315 (82.22%), Query Frame = 0


Query: 11  LKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAI 70
           ++ S+S   V MP++G GTAAD  D + + +AV E+I+LGYRHFDTA++YGSEQ LGEAI
Sbjct: 1   MELSSSSGPVSMPVIGFGTAADNNDGATMISAVLESIKLGYRHFDTASAYGSEQALGEAI 60

Query: 71  AEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKP 130
           AEAL LGL++SR+++F+TSKLW  +AH + VLP+L+ SLRTL+LEYLDLYL+HWPI++ P
Sbjct: 61  AEALRLGLIASRDDLFITSKLWPSEAHAHLVLPSLQKSLRTLQLEYLDLYLIHWPITVTP 120

Query: 131 GKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVN 190
           GK +F     DL+PMD +SVW  MEECQRLGL ++IG+ NFSCKKL+NLLSF+ IPP+VN
Sbjct: 121 GKREFPFDKQDLMPMDFKSVWAAMEECQRLGLTKSIGVSNFSCKKLENLLSFATIPPSVN 180

Query: 191 QVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVA 250
           QVEM+PVWQQ+KL EFC +NGI+VTA+SPLGAKG+SWGT+ VMD E+LK+IA+   +TVA
Sbjct: 181 QVEMNPVWQQKKLTEFCKANGIIVTAFSPLGAKGSSWGTNDVMDNEVLKEIAKTHGKTVA 240

Query: 251 QVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISA 310
           QV LRWVYEQG   +VKS NK R++ENL IFDW L+E+ +  I QIKQ R + + + +SA
Sbjct: 241 QVCLRWVYEQGAVPIVKSYNKERLKENLQIFDWELSEDDYDKIGQIKQHRMMPKEELVSA 300

Query: 311 HGPYKSIQQLWDGEL 326
           HGPYKS+++LWDGEL
Sbjct: 301 HGPYKSVEELWDGEL 315

[Close]

BLAST of MS000922 vs. ExPASy Swiss-Prot
Match: Q9SQ64 (Non-functional NADPH-dependent codeinone reductase 2 OS=Papaver somniferum OX=3469 GN=COR2 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.3e-107
Identity = 185/321 (57.63%), Postives = 246/321 (76.64%), Query Frame = 0


Query: 6   MPTVSLKSSASGHSVRMPILGMGTAADPFDAS-ALKAAVPEAIRLGYRHFDTAASYGSEQ 65
           +P V+L S        MPILGMGTA +    S  +K A+ +AI +GYRHFDTA  Y +E 
Sbjct: 6   VPVVTLSSGRG-----MPILGMGTAENNLQGSERVKLAILKAIEVGYRHFDTAFVYQTEG 65

Query: 66  PLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHW 125
            LGEA+AEAL+ GL+ SR+E+F+TSKLWC DA+P+ VLPAL+NSLR LKLEYLDLYL+HW
Sbjct: 66  SLGEAVAEALQNGLIKSRDELFITSKLWCADAYPDHVLPALQNSLRNLKLEYLDLYLIHW 125

Query: 126 PISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSK 185
           P+SLKPGK    I   ++ P+D +SVW  ME+CQ LGL ++IG+ NFSCKKL  L++ + 
Sbjct: 126 PVSLKPGKFVHPIPKDEIFPIDYKSVWAAMEKCQMLGLTKSIGVSNFSCKKLHYLMATAN 185

Query: 186 IPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEA 245
           IPPAVNQVEM+P+WQQ+KLR++C +N I+VTAYSPLGAKG  WG+S VMD E+L  I++ 
Sbjct: 186 IPPAVNQVEMNPIWQQQKLRDYCKTNNIMVTAYSPLGAKGTMWGSSGVMDSEVLNQISQV 245

Query: 246 THRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSR 305
             ++VAQVSLRWVYEQG +LLVKS N+ RM+ENL IFDW L+ E  ++I+++ Q+R  + 
Sbjct: 246 RGKSVAQVSLRWVYEQGASLLVKSFNEERMKENLKIFDWELSPEDLKNISELPQRRVSTG 305

Query: 306 HDFISAHGPYKSIQQLWDGEL 326
             F+S +GP+KS+++LWD E+
Sbjct: 306 DPFVSINGPFKSVEELWDDEV 321

[Close]

BLAST of MS000922 vs. ExPASy Swiss-Prot
Match: A0A1D5XGW0 (Deoxymugineic acid synthase 1-B OS=Triticum aestivum OX=4565 GN=DMAS1-B PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-104
Identity = 176/304 (57.89%), Postives = 228/304 (75.00%), Query Frame = 0


Query: 22  MPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLLSS 81
           MP +GMGTA     A  ++ AV  AI++GYRHFDTAA Y +E P+GEA AEA+  G ++S
Sbjct: 11  MPRIGMGTAVQGPKADPIRRAVLRAIQIGYRHFDTAAHYETEAPIGEAAAEAVRSGAVAS 70

Query: 82  REEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHSSD 141
           R+E+F+TSKLWC DAH + V+PALR +LR L++EY+DLYLVHWP+S+KPG+      + D
Sbjct: 71  RDELFITSKLWCSDAHRDRVVPALRQTLRNLQMEYVDLYLVHWPVSMKPGRFKAPFTAED 130

Query: 142 LVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQQR 201
            VP D+ +VW  MEEC RLGLA+AIG+ NFSCKKL+ LLSF+ IPP VNQVE++PVWQQR
Sbjct: 131 FVPFDMRAVWEAMEECHRLGLAKAIGVANFSCKKLETLLSFATIPPTVNQVEVNPVWQQR 190

Query: 202 KLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYEQG 261
           KLREFC   GI + AYSPLGAKG  WG+  VMD  +L++IA +  ++VAQV LRWVYEQG
Sbjct: 191 KLREFCRGKGIQLCAYSPLGAKGTHWGSDAVMDAGVLQEIAASRGKSVAQVCLRWVYEQG 250

Query: 262 VTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISAHGPYKSIQQLW 321
             L+VKS ++ RM ENL +  W LTEE  R I +I Q++      ++S HGPYKS+++LW
Sbjct: 251 DCLIVKSFDEARMRENLDVDGWELTEEERRRIAEIPQRKINLGKRYVSEHGPYKSLEELW 310

Query: 322 DGEL 326
           DGE+
Sbjct: 311 DGEI 314

[Close]

BLAST of MS000922 vs. ExPASy Swiss-Prot
Match: W5DYE3 (Deoxymugineic acid synthase 1-A OS=Triticum aestivum OX=4565 GN=DMAS1-A PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.4e-104
Identity = 175/304 (57.57%), Postives = 227/304 (74.67%), Query Frame = 0


Query: 22  MPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLLSS 81
           MP +GMGTA        ++ AV  AI +GYRHFDTAA Y +E P+GEA AEA+  G ++S
Sbjct: 11  MPRIGMGTAVQGPKPDPIRRAVLRAIEVGYRHFDTAAHYETEAPIGEAAAEAVRSGAVAS 70

Query: 82  REEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHSSD 141
           R+++F+TSKLWC DAH + V+PALR +LR L++EY+DLYLVHWP+S+KPG+      + D
Sbjct: 71  RDDLFITSKLWCSDAHRDRVVPALRQTLRNLQMEYVDLYLVHWPVSMKPGRFKAPFTAED 130

Query: 142 LVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQQR 201
            VP D+ +VW  MEEC RLGLA+AIG+ NFSCKKL+ LLSF+ IPP VNQVE++PVWQQR
Sbjct: 131 FVPFDMRAVWEAMEECHRLGLAKAIGVANFSCKKLETLLSFATIPPTVNQVEVNPVWQQR 190

Query: 202 KLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYEQG 261
           KLREFC   GI + AYSPLGAKG  WG+  VMD  +L++IA +  ++VAQV LRWVYEQG
Sbjct: 191 KLREFCRGKGIQLCAYSPLGAKGTHWGSDAVMDAGVLQEIAASRGKSVAQVCLRWVYEQG 250

Query: 262 VTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISAHGPYKSIQQLW 321
             L+VKS ++ RM ENL +  W LTEE HR I +I Q++      ++S HGPYKS+++LW
Sbjct: 251 DCLIVKSFDEARMRENLDVDGWELTEEEHRRIAEIPQRKINLGKRYVSEHGPYKSLEELW 310

Query: 322 DGEL 326
           DGE+
Sbjct: 311 DGEI 314

[Close]

BLAST of MS000922 vs. ExPASy Swiss-Prot
Match: B4F9A4 (Deoxymugineic acid synthase 1 OS=Zea mays OX=4577 GN=DMAS1 PE=1 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.3e-104
Identity = 177/313 (56.55%), Postives = 234/313 (74.76%), Query Frame = 0


Query: 14  SASGHS-VRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAE 73
           SA+G +   +P +G+GTA        ++AAV  AI+LGYRHFDTAA Y +E P+GEA AE
Sbjct: 2   SATGRAPCGLPRIGLGTAVQGPRPDPVRAAVLRAIQLGYRHFDTAAHYATEAPIGEAAAE 61

Query: 74  ALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGK 133
           A+  GL++SRE++FVTSK+WC DAH + VLPALR +L  L++EY+DLY+VHWP+++K G+
Sbjct: 62  AVRTGLVASREDLFVTSKVWCADAHRDRVLPALRRTLSNLQMEYVDLYMVHWPVTMKAGR 121

Query: 134 DDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQV 193
                   D  P D+ +VW  MEEC RLGLA+AIG+CNFSCKKL+ LLSF+ IPP VNQV
Sbjct: 122 FTAPFTPEDFEPFDMRAVWEAMEECHRLGLAKAIGVCNFSCKKLETLLSFATIPPVVNQV 181

Query: 194 EMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQV 253
           E++PVWQQRKLREFC + GI + AYSPLGAKG  WG+  VMD  +L +IA++  +TVAQV
Sbjct: 182 EINPVWQQRKLREFCRAKGIQLCAYSPLGAKGTHWGSDSVMDSGVLHEIAKSKGKTVAQV 241

Query: 254 SLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISAHG 313
            LRWVYEQG  L+VKS ++ RM+ENL I DW L+EE  + I++I Q++      ++S HG
Sbjct: 242 CLRWVYEQGDCLIVKSFDEGRMKENLDIVDWELSEEERQRISKIPQRKINQGRRYVSEHG 301

Query: 314 PYKSIQQLWDGEL 326
           PYKS ++LWDGE+
Sbjct: 302 PYKSFEELWDGEI 314

[Close]

BLAST of MS000922 vs. ExPASy Swiss-Prot
Match: Q0PCF4 (Deoxymugineic acid synthase 1 OS=Hordeum vulgare OX=4513 GN=DMAS1 PE=1 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 9.0e-104
Identity = 175/304 (57.57%), Postives = 226/304 (74.34%), Query Frame = 0


Query: 22  MPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLLSS 81
           MP +GMGTA        ++ AV  AI +GYRHFDTAA Y +E P+GEA AEA+  G ++S
Sbjct: 11  MPRIGMGTAVQGPKPDPIRRAVLRAIEIGYRHFDTAAHYETEAPIGEAAAEAVRSGAVAS 70

Query: 82  REEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHSSD 141
           R+++F+TSKLWC DAH + V+PALR++LR L++EY+DLYLVHWP+S+KPG+      + D
Sbjct: 71  RDDLFITSKLWCSDAHGDRVVPALRHTLRNLQMEYVDLYLVHWPVSMKPGRFKAPFTAED 130

Query: 142 LVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQQR 201
            VP D+ +VW  MEEC RLGLA+AIG+ NFSCKKL  LLSF+ IPP VNQVE++PVWQQR
Sbjct: 131 FVPFDMRAVWEAMEECHRLGLAKAIGVANFSCKKLDTLLSFATIPPTVNQVEVNPVWQQR 190

Query: 202 KLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYEQG 261
           KLREFC   GI + AYSPLGAKG  WG+  VMD  +L+DIA +  ++VAQV LRWVYEQG
Sbjct: 191 KLREFCRGKGIQLCAYSPLGAKGTHWGSDAVMDAGVLQDIAASRGKSVAQVCLRWVYEQG 250

Query: 262 VTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISAHGPYKSIQQLW 321
             L+VKS ++ RM ENL +  W LTEE  R I +I Q++      ++S HGPYKS+++LW
Sbjct: 251 DCLIVKSFDEARMRENLDVDGWELTEEERRRIAEIPQRKINLGKRYVSDHGPYKSLEELW 310

Query: 322 DGEL 326
           DGE+
Sbjct: 311 DGEI 314

[Close]

BLAST of MS000922 vs. ExPASy TrEMBL
Match: A0A6J1BVS8 (non-functional NADPH-dependent codeinone reductase 2-like OS=Momordica charantia OX=3673 GN=LOC111005164 PE=4 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 7.5e-186
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 0


Query: 1   MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASY 60
           MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASY
Sbjct: 13  MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASY 72

Query: 61  GSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLY 120
           GSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLY
Sbjct: 73  GSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLY 132

Query: 121 LVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLL 180
           LVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIG+CNFSCKKLQNLL
Sbjct: 133 LVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGVCNFSCKKLQNLL 192

Query: 181 SFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKD 240
           SFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKD
Sbjct: 193 SFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKD 252

Query: 241 IAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQR 300
           IAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQR
Sbjct: 253 IAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQR 312

Query: 301 GLSRHDFISAHGPYKSIQQLWDGEL 326
           GLSRHDFISAHGPYKSIQQLWDGEL
Sbjct: 313 GLSRHDFISAHGPYKSIQQLWDGEL 337

[Close]

BLAST of MS000922 vs. ExPASy TrEMBL
Match: A0A660KQ25 (Aldo_ket_red domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_010386 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 1.2e-119
Identity = 208/317 (65.62%), Postives = 259/317 (81.70%), Query Frame = 0


Query: 9   VSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGE 68
           V L SS+ G+   M ++G G AAD  D + LK+AV EAI+LGYRHF+TA++YGSEQ LGE
Sbjct: 10  VVLSSSSFGNKA-MSVIGFGMAADSNDGATLKSAVLEAIKLGYRHFNTASAYGSEQALGE 69

Query: 69  AIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISL 128
            IA+AL+LGL+SSREE+F+TSKLW  DAHP+ VLP L  S+RTL+LEY+DLYL+HWPI++
Sbjct: 70  GIAQALKLGLVSSREELFITSKLWSSDAHPDLVLPTLHKSVRTLQLEYIDLYLIHWPITV 129

Query: 129 KPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPA 188
           KPGK  +     D++PMD +SVW  MEECQRLGLA+ IG+ NFSCKKLQNLLS S IPPA
Sbjct: 130 KPGKGGWPFAEEDMMPMDFKSVWAAMEECQRLGLAKFIGVSNFSCKKLQNLLSSSTIPPA 189

Query: 189 VNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRT 248
           VNQVEMSPVWQQ+KLR+FC  NGIVVTA+SPLGAKG SWGT+ VM+ E+L DIA+A  +T
Sbjct: 190 VNQVEMSPVWQQKKLRDFCKENGIVVTAFSPLGAKGTSWGTNHVMENEVLMDIAKARGKT 249

Query: 249 VAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFI 308
           VAQV LRW+YEQGVTL++KS NK R++ENL IFDW L+E+  + I++IKQ R + + + I
Sbjct: 250 VAQVCLRWMYEQGVTLVMKSYNKERLKENLQIFDWELSEDDSKKISEIKQHRMMLKEELI 309

Query: 309 SAHGPYKSIQQLWDGEL 326
           SA GPYKSI++LWDGEL
Sbjct: 310 SARGPYKSIEELWDGEL 325

[Close]

BLAST of MS000922 vs. ExPASy TrEMBL
Match: A0A059B141 (Aldo_ket_red domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_H02498 PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 4.6e-119
Identity = 204/320 (63.75%), Postives = 260/320 (81.25%), Query Frame = 0


Query: 6   MPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQP 65
           +P V L S+A+G S  MP++G+GTAADP DA+A+KAAV EAI+LGYRHFDTA+ YGSE+P
Sbjct: 12  VPEVPLGSAAAG-SRPMPVIGLGTAADPLDAAAMKAAVLEAIKLGYRHFDTASLYGSEEP 71

Query: 66  LGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWP 125
           LGE +AEAL LGL+ SR E+FVTSKLW  DAH + V+PALR SL  L+L+YLDLYL+HWP
Sbjct: 72  LGEVLAEALGLGLVGSRAELFVTSKLWSSDAHADLVVPALRKSLSNLQLDYLDLYLIHWP 131

Query: 126 ISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKI 185
           IS KPG   F    SDL+PMD E+VW  MEECQRLGL ++IG+ NFSCKKL N+LSF+KI
Sbjct: 132 ISCKPGLYGFPEDKSDLMPMDFEAVWAAMEECQRLGLTKSIGVSNFSCKKLGNILSFAKI 191

Query: 186 PPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEAT 245
           PP VNQVEM+P+WQQ+KLREFC +N +V+TA+SPLGA+G  WGT++VM+ E LK+IA A 
Sbjct: 192 PPTVNQVEMNPLWQQKKLREFCKANNVVITAFSPLGARGTCWGTNEVMENESLKEIARAR 251

Query: 246 HRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRH 305
            +++AQV LRW+YE+GVT +VKS NK R+ ENL+IFDW LTE  H  I +I Q+R + + 
Sbjct: 252 GKSIAQVCLRWLYERGVTFVVKSFNKERLNENLNIFDWELTEGDHEKIERIPQKRMMLKE 311

Query: 306 DFISAHGPYKSIQQLWDGEL 326
           +F+SA GP+KS+++LWDGEL
Sbjct: 312 EFVSAKGPFKSVEELWDGEL 330

[Close]

BLAST of MS000922 vs. ExPASy TrEMBL
Match: A0A2N9H0D5 (Aldo_ket_red domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS33344 PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 4.6e-119
Identity = 209/322 (64.91%), Postives = 261/322 (81.06%), Query Frame = 0


Query: 4   TTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSE 63
           T +P V L SS+    V MP++G GTAAD  D S L +AV E+I+LGY HFDTA++YGSE
Sbjct: 10  TNIPKVELTSSSG--PVAMPMIGFGTAADSNDGSTLISAVQESIKLGYTHFDTASAYGSE 69

Query: 64  QPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVH 123
           Q LGEAI +AL  GL++SR+++F+TSKLW  DAH + VLP+L+ SLRTL+LEYLDLYL+H
Sbjct: 70  QALGEAIDQALTHGLVTSRDDLFITSKLWPCDAHAHLVLPSLQKSLRTLRLEYLDLYLIH 129

Query: 124 WPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFS 183
           WPIS+ PGK +F     DL+PMD +SVW  MEECQRLGL ++IG+ NFSCKKL+NLLSF+
Sbjct: 130 WPISITPGKWEFPFDKQDLMPMDFKSVWAAMEECQRLGLTKSIGVSNFSCKKLENLLSFA 189

Query: 184 KIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAE 243
            IPP+VNQVEM+PVWQQ+KL EFC +NGI+VTA+SPLGAKG+SWGT+ VMD E LK+IA+
Sbjct: 190 TIPPSVNQVEMNPVWQQKKLIEFCKANGIIVTAFSPLGAKGSSWGTNDVMDNEALKEIAK 249

Query: 244 ATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLS 303
           A  +TVAQV LRWVYEQGV  +VKS NK R++ENL IFDWAL+E+ +  I QIKQ R + 
Sbjct: 250 ARGKTVAQVCLRWVYEQGVAPIVKSYNKERLKENLQIFDWALSEDDYDKIGQIKQHRMML 309

Query: 304 RHDFISAHGPYKSIQQLWDGEL 326
           + + ISAHGPYKSI++LWDGEL
Sbjct: 310 KEELISAHGPYKSIEELWDGEL 329

[Close]

BLAST of MS000922 vs. ExPASy TrEMBL
Match: A0A2P5FMN3 (Aldo_ket_red domain-containing protein (Fragment) OS=Trema orientale OX=63057 GN=TorRG33x02_052920 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.3e-118
Identity = 210/325 (64.62%), Postives = 257/325 (79.08%), Query Frame = 0


Query: 1   MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASY 60
           M ST +P + L SS       MP++G+GTA++  D SALK AV EAI+LGYRHFDTA+ Y
Sbjct: 13  MASTDIPNLVLGSSTGLKG--MPVIGLGTASEDRDPSALKRAVVEAIKLGYRHFDTASLY 72

Query: 61  GSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLY 120
           GSEQ LGEAIAE+L+LGL++SR+E+F+TSKLW  DAHP+ V+P L+ SL  L+LE LDLY
Sbjct: 73  GSEQGLGEAIAESLKLGLVASRDELFITSKLWITDAHPDLVIPTLKKSLGNLQLESLDLY 132

Query: 121 LVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLL 180
           L+HWPIS KPGK  F I   DL+PMD ESVWG MEECQRLGL R+IG+ NFSCKKL N+L
Sbjct: 133 LIHWPISAKPGKRLFPIDEKDLMPMDFESVWGAMEECQRLGLTRSIGVSNFSCKKLDNIL 192

Query: 181 SFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKD 240
           SF+ IPP+VNQVE++P WQQ+KLREFC SN I+VTAYSPLGAKG  WGT+ V+D E+LKD
Sbjct: 193 SFATIPPSVNQVELNPTWQQQKLREFCGSNNIIVTAYSPLGAKGTRWGTNDVLDNEVLKD 252

Query: 241 IAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQR 300
           IA A  +TVAQVSLRW+YEQG TL+VKS NK R++ENL IFDWAL+E   + I  I Q +
Sbjct: 253 IAIARGKTVAQVSLRWIYEQGATLVVKSYNKERLKENLQIFDWALSEHDIQKIKLIPQHK 312

Query: 301 GLSRHDFISAHGPYKSIQQLWDGEL 326
                 F+SAHGPYKS+++LWDGE+
Sbjct: 313 VNVNTAFVSAHGPYKSLEELWDGEI 335

[Close]

BLAST of MS000922 vs. TAIR 10
Match: AT1G59960.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 411.4 bits (1056), Expect = 6.8e-115
Identity = 194/327 (59.33%), Postives = 252/327 (77.06%), Query Frame = 0


Query: 1   MMSTTMPTVSLKSSASGHSVRMPILGMGTAADPF-DASALKAAVPEAIRLGYRHFDTAAS 60
           M  TT+PT++++S  SGH   MP+LG GTAA P  + + LK  V EAI+LGYRHFDT+  
Sbjct: 1   MSLTTVPTLAIRSGPSGHH-SMPVLGFGTAASPLPEPTMLKETVIEAIKLGYRHFDTSPR 60

Query: 61  YGSEQPLGEAIAEALELGLLSSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDL 120
           Y +E+P+GEA+AEA+ LGL+ SR E FVT+KLWC DAH   V+PA++ SL+ LKL+YLDL
Sbjct: 61  YQTEEPIGEALAEAVSLGLVRSRSEFFVTTKLWCADAHGGLVVPAIKRSLKNLKLDYLDL 120

Query: 121 YLVHWPISLKPGKDDFSIHSSDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNL 180
           Y++HWP+S KPGK  F I   D +PMD E VW  MEECQRLGLA+ IG+ NFSCKKLQ++
Sbjct: 121 YIIHWPVSSKPGKYKFPIDEDDFMPMDFEVVWSEMEECQRLGLAKCIGVSNFSCKKLQHI 180

Query: 181 LSFSKIPPAVNQVEMSPVWQQRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILK 240
           LS + IPP+VNQVEMSP+WQQRKLRE C SN IVVTAYS LG++GA WGT ++M+ ++LK
Sbjct: 181 LSIATIPPSVNQVEMSPIWQQRKLRELCRSNDIVVTAYSVLGSRGAFWGTPKIMESDVLK 240

Query: 241 DIAEATHRTVAQVSLRWVYEQGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSIN-QIKQ 300
           +IAEA  +TVAQVS+RW YEQGV+++VKS  K R+EENL IFDW+LTE+  + I+ +I Q
Sbjct: 241 EIAEAKEKTVAQVSMRWAYEQGVSMVVKSFTKERLEENLKIFDWSLTEDETQRISTEIPQ 300

Query: 301 QRGLSRHDFISAHGPYKSIQQLWDGEL 326
            R +    + S  GP KS+ ++WDGE+
Sbjct: 301 FRNVHGEVYTSKKGPIKSVAEMWDGEI 326

[Close]

BLAST of MS000922 vs. TAIR 10
Match: AT1G59950.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 404.4 bits (1038), Expect = 8.4e-113
Identity = 190/306 (62.09%), Postives = 241/306 (78.76%), Query Frame = 0


Query: 22  MPILGMGTAAD-PFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLLS 81
           MP+L +GTAA  P +   LK  V EAI+LGYRHFDT+  Y +E+PLGEA+AEA+ LGL+ 
Sbjct: 15  MPVLALGTAASPPPEPIVLKRTVLEAIKLGYRHFDTSPRYQTEEPLGEALAEAVSLGLIQ 74

Query: 82  SREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHSS 141
           SR E+FVTSKLWC DAH   V+PA++ SL TLKL+YLDLYL+HWP+S KPGK  F I   
Sbjct: 75  SRSELFVTSKLWCADAHGGLVVPAIQRSLETLKLDYLDLYLIHWPVSSKPGKYKFPIEED 134

Query: 142 DLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQQ 201
           D +PMD E+VW  MEECQRLG+A+ IG+ NFSCKKLQ++LS +KIPP+VNQVEMSPVWQQ
Sbjct: 135 DFLPMDYETVWSEMEECQRLGVAKCIGVSNFSCKKLQHILSIAKIPPSVNQVEMSPVWQQ 194

Query: 202 RKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYEQ 261
           RKLRE C S GIVVTAYS LG++GA WGT ++M+ ++LK+IAEA  +TVAQVS+RW YE+
Sbjct: 195 RKLRELCKSKGIVVTAYSVLGSRGAFWGTHKIMESDVLKEIAEAKGKTVAQVSMRWAYEE 254

Query: 262 GVTLLVKSTNKRRMEENLSIFDWALTEEHHRSIN-QIKQQRGLSRHDFISAHGPYKSIQQ 321
           GV+++VKS  K R+EENL IFDW+LTEE  + I+ +I Q R +    +IS  GP KS+ +
Sbjct: 255 GVSMVVKSFRKDRLEENLKIFDWSLTEEEKQRISTEISQSRIVDGEVYISEKGPIKSVTE 314

Query: 322 LWDGEL 326
           +WDGE+
Sbjct: 315 MWDGEI 320

[Close]

BLAST of MS000922 vs. TAIR 10
Match: AT5G62420.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 271.6 bits (693), Expect = 8.5e-73
Identity = 143/308 (46.43%), Postives = 198/308 (64.29%), Query Frame = 0


Query: 22  MPILGMGTAADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLLSS 81
           +P+LGMGT     D  +  +AV +AI++GYRHFDTA  YGSE+ LG A+ +A+  G +  
Sbjct: 14  IPLLGMGTYCPQKDRESTISAVHQAIKIGYRHFDTAKIYGSEEALGTALGQAISYGTV-Q 73

Query: 82  REEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHSSD 141
           R+++FVTSKLW  D H    + AL  +L+T+ L+YLD YLVHWPI LKPG  +  I   D
Sbjct: 74  RDDLFVTSKLWSSDHH--DPISALIQTLKTMGLDYLDNYLVHWPIKLKPGVSE-PIPKED 133

Query: 142 LVPMDL--ESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQ 201
               DL  E  W GME C  +GL R+IG+ NFS KK+ +LL F+ + P+VNQVEM P+W+
Sbjct: 134 EFEKDLGIEETWQGMERCLEMGLCRSIGVSNFSSKKIFDLLDFASVSPSVNQVEMHPLWR 193

Query: 202 QRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYE 261
           QRKLR+ C  N I V+ YSPLG  G  WG++ V++  I+K IA   + T AQV+LRW   
Sbjct: 194 QRKLRKVCEENNIHVSGYSPLGGPGNCWGSTAVIEHPIIKSIALKHNATPAQVALRWGMS 253

Query: 262 QGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFI--SAHGPYKSI 321
           +G +++VKS N  RM EN    +  L ++    I+ +++ + + R DF+      PYKSI
Sbjct: 254 KGASVIVKSFNGARMIENKRALEIKLDDQDLSLIDHLEEWK-IMRGDFLVNQTTSPYKSI 313

Query: 322 QQLWDGEL 326
           QQLWD E+
Sbjct: 314 QQLWDNEI 316

[Close]

BLAST of MS000922 vs. TAIR 10
Match: AT2G37790.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.5e-72
Identity = 133/306 (43.46%), Postives = 198/306 (64.71%), Query Frame = 0


Query: 21  RMPILGMGT-AADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLL 80
           ++P +G+GT  ADP     +  AV  A+++GYRH D A  YG+E+ +G  + +  + G++
Sbjct: 15  KIPSVGLGTWQADP---GLVGNAVDAAVKIGYRHIDCAQIYGNEKEIGLVLKKLFDGGVV 74

Query: 81  SSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHS 140
             REE+F+TSKLWC    P  V  AL  +L+ L+L+Y+DLYL+HWP+SLK G   F    
Sbjct: 75  -KREEMFITSKLWCTYHDPQEVPEALNRTLQDLQLDYVDLYLIHWPVSLKKGSTGFK--P 134

Query: 141 SDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQ 200
            +++P D+ S W  ME     G ARAIG+ NFS KKL +LL  +++PPAVNQVE  P WQ
Sbjct: 135 ENILPTDIPSTWKAMESLFDSGKARAIGVSNFSSKKLADLLVVARVPPAVNQVECHPSWQ 194

Query: 201 QRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYE 260
           Q  LR+FC S G+ ++ YSPLG+ G +W TS V+   IL  +AE   +T AQV+LRW  +
Sbjct: 195 QNVLRDFCKSKGVHLSGYSPLGSPGTTWLTSDVLKNPILGGVAEKLGKTPAQVALRWGLQ 254

Query: 261 QGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSRHDFISAHGPYKSIQQ 320
            G ++L KST++ R+++N  +F+W++ E+     ++I Q R +    F+    PYKS+++
Sbjct: 255 MGQSVLPKSTHEDRIKQNFDVFNWSIPEDMLSKFSEIGQGRLVRGMSFVHETSPYKSLEE 314

Query: 321 LWDGEL 326
           LWDGE+
Sbjct: 315 LWDGEI 314

[Close]

BLAST of MS000922 vs. TAIR 10
Match: AT2G37770.2 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.4e-70
Identity = 135/310 (43.55%), Postives = 198/310 (63.87%), Query Frame = 0


Query: 21  RMPILGMGT-AADPFDASALKAAVPEAIRLGYRHFDTAASYGSEQPLGEAIAEALELGLL 80
           + P +G+GT  A P     +  AV  A+++GYRH D A  YG+E+ +G  + +  E   +
Sbjct: 15  KFPSVGLGTWQASP---GLVGDAVAAAVKIGYRHIDCAQIYGNEKEIGAVLKKLFE-DRV 74

Query: 81  SSREEVFVTSKLWCGDAHPNTVLPALRNSLRTLKLEYLDLYLVHWPISLKPGKDDFSIHS 140
             RE++F+TSKLWC D  P  V  AL  +L+ L+LEY+DLYL+HWP  +K G     I  
Sbjct: 75  VKREDLFITSKLWCTDHDPQDVPEALNRTLKDLQLEYVDLYLIHWPARIKKG--SVGIKP 134

Query: 141 SDLVPMDLESVWGGMEECQRLGLARAIGICNFSCKKLQNLLSFSKIPPAVNQVEMSPVWQ 200
            +L+P+D+ S W  ME     G ARAIG+ NFS KKL +LL  +++PPAVNQVE  P W+
Sbjct: 135 ENLLPVDIPSTWKAMEALYDSGKARAIGVSNFSTKKLADLLELARVPPAVNQVECHPSWR 194

Query: 201 QRKLREFCNSNGIVVTAYSPLGAKGASWGTSQVMDCEILKDIAEATHRTVAQVSLRWVYE 260
           Q KL+EFC S G+ ++AYSPLG+ G +W  S V+   IL  +AE   ++ AQV+LRW  +
Sbjct: 195 QTKLQEFCKSKGVHLSAYSPLGSPGTTWLKSDVLKNPILNMVAEKLGKSPAQVALRWGLQ 254

Query: 261 QGVTLLVKSTNKRRMEENLSIFDWALTEEHHRSINQIKQQRGLSR----HDFISAHGPYK 320
            G ++L KSTN+ R++EN ++FDW++ +       +I+Q R ++     H+ +S   PYK
Sbjct: 255 MGHSVLPKSTNEGRIKENFNVFDWSIPDYMFAKFAEIEQARLVTGSFLVHETLS---PYK 314

Query: 321 SIQQLWDGEL 326
           SI++LWDGE+
Sbjct: 315 SIEELWDGEI 315

The following BLAST results are available for this feature:

BLAST of MS000922 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132268.1	1.5e-185	99.69	non-functional NADPH-dependent codeinone reductase 2-like [Momordica charantia]	[more]
KAG8640091.1	5.1e-120	64.89	hypothetical protein MANES_13G023400v8 [Manihot esculenta]	[more]
KAE8037831.1	2.5e-119	65.62	hypothetical protein FH972_010386 [Carpinus fangiana]	[more]
KAF3966331.1	4.3e-119	64.13	hypothetical protein CMV_009552 [Castanea mollissima]	[more]
KAF3960187.1	7.3e-119	63.49	hypothetical protein CMV_015076 [Castanea mollissima]	[more]

BLAST of MS000922 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SQ64	1.3e-107	57.63	Non-functional NADPH-dependent codeinone reductase 2 OS=Papaver somniferum OX=34...	[more]
A0A1D5XGW0	1.4e-104	57.89	Deoxymugineic acid synthase 1-B OS=Triticum aestivum OX=4565 GN=DMAS1-B PE=2 SV=...	[more]
W5DYE3	2.4e-104	57.57	Deoxymugineic acid synthase 1-A OS=Triticum aestivum OX=4565 GN=DMAS1-A PE=1 SV=...	[more]
B4F9A4	5.3e-104	56.55	Deoxymugineic acid synthase 1 OS=Zea mays OX=4577 GN=DMAS1 PE=1 SV=1	[more]
Q0PCF4	9.0e-104	57.57	Deoxymugineic acid synthase 1 OS=Hordeum vulgare OX=4513 GN=DMAS1 PE=1 SV=1	[more]

BLAST of MS000922 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVS8	7.5e-186	99.69	non-functional NADPH-dependent codeinone reductase 2-like OS=Momordica charantia...	[more]
A0A660KQ25	1.2e-119	65.62	Aldo_ket_red domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_0...	[more]
A0A059B141	4.6e-119	63.75	Aldo_ket_red domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ...	[more]
A0A2N9H0D5	4.6e-119	64.91	Aldo_ket_red domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS3...	[more]
A0A2P5FMN3	2.3e-118	64.62	Aldo_ket_red domain-containing protein (Fragment) OS=Trema orientale OX=63057 GN...	[more]

BLAST of MS000922 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G59960.1	6.8e-115	59.33	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT1G59950.1	8.4e-113	62.09	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT5G62420.1	8.5e-73	46.43	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT2G37790.1	2.5e-72	43.46	NAD(P)-linked oxidoreductase superfamily protein	[more]
AT2G37770.2	1.4e-70	43.55	NAD(P)-linked oxidoreductase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020471	Aldo-keto reductase	PRINTS	PR00069	ALDKETRDTASE	coord: 154..171 score: 48.24 coord: 107..125 score: 60.74 coord: 46..70 score: 54.07 coord: 188..217 score: 50.43 coord: 232..256 score: 35.2
IPR020471	Aldo-keto reductase	PIRSF	PIRSF000097	AKR	coord: 4..319 e-value: 8.7E-84 score: 279.0
IPR023210	NADP-dependent oxidoreductase domain	PFAM	PF00248	Aldo_ket_red	coord: 25..296 e-value: 3.2E-50 score: 171.0
IPR036812	NADP-dependent oxidoreductase domain superfamily	GENE3D	3.20.20.100		coord: 6..311 e-value: 4.3E-103 score: 346.3
IPR036812	NADP-dependent oxidoreductase domain superfamily	SUPERFAMILY	51430	NAD(P)-linked oxidoreductase	coord: 19..309
None	No IPR available	PIRSR	PIRSR000097-3	PIRSR000097-3	coord: 20..126 e-value: 1.0E-28 score: 97.9
None	No IPR available	PIRSR	PIRSR000097-2	PIRSR000097-2	coord: 20..126 e-value: 1.0E-28 score: 97.9
None	No IPR available	PANTHER	PTHR11732	ALDO/KETO REDUCTASE	coord: 14..323
None	No IPR available	PANTHER	PTHR11732:SF416	ALDO/KETO REDUCTASE FAMILY OXIDOREDUCTASE	coord: 14..323
IPR018170	Aldo/keto reductase, conserved site	PROSITE	PS00798	ALDOKETO_REDUCTASE_1	coord: 50..67
IPR018170	Aldo/keto reductase, conserved site	PROSITE	PS00063	ALDOKETO_REDUCTASE_3	coord: 265..280
IPR018170	Aldo/keto reductase, conserved site	PROSITE	PS00062	ALDOKETO_REDUCTASE_2	coord: 154..171
IPR044497	Aldo-keto reductase family 4A/B	CDD	cd19124	AKR_AKR4A_4B	coord: 16..298 e-value: 0.0 score: 514.125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000922.1	MS000922.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0047834	D-threo-aldose 1-dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor