MS000921 (gene) Bitter gourd (TR) v1

Overview


Name	MS000921
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold36: 275767 .. 277998 (+)
RNA-Seq Expression	MS000921
Synteny	MS000921

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGTTCCAAATGGCGTGCGATTCTCAACCTCCTTCCCTGTCTCCTTCCTTTGTTCTCCCGGAGGACATAAGGCCACGTCTCCAACAAGTGTCACACGGCATCTCTGTTCCGGTCATCGACTTGGCCGCCGATGATCAATCCCTTGTGGTTGGAAAAGTATCGGAAGCCTGCCAGCAGTACGGCTTCTTCCAGGTCATCAATCACGGAATCCCGCAAGCCTTGTGCGACAGAGTACTGGACACAGTCGCCCAATTCTTCCACCTCCCTCCCCAACACAGGAACCAATACGTCACCACCGACCACAAGAAGAAGGTTAAAATCTTCAATTACTACGCCAACGTGGAGGGGCGGAGGGTCAACATGTGGAGCGAAACCTTCACGCTTCCCTGGAATCCCGATCCCGTGGAGACGGAGTATCTGGACGTGGTGCCCGAAGAGCCAGCCGAATACAGGGAAGTTTACGGGGAGTACGCGAGGGAGATGGGAAGCCTAATGGAGAGGCTTCTGGGGTTGATGTCGTTGGGGCTGGGCCTGGAGGAGGGCGCGCTCAAGAGGCGCATCGGGGCCAAGCCGGAGCTGTACTCGCAGGCCAACTACTACCCGCCCTGTCCGGACCCAGAGCTCACCATGGGCCTTGGGGCCCATACTGATATTGTAGCCATTGCGGTGCTGTTGCAGAACCGAGCCCCCAGTGGGCTCCATATCATGAAAGATGACCACTGGGTCTCCGTCGATCCTATTCCCAATTCACTTGTCGTCAATTTGGCTGATCAAGTCCAGGTACGCCAACGCCACACACACAACCACCATTCACCATTATCATACTTTTTCTTTTCCTTTTCCCTCTTACAATTACAACAAATCATTCATTCTTGATTATTTCTTGCGTAGTCAACACCAACCTATACATAAAGACGTTGACTTTATTAATCTGACTTGACACCATATTCATTGTATAAGTGGAAGAAAAAAAGCACAGAGGCTTCCTAACAGTATTGAATTGTACATGATTGCTAATTAAAACAGGTTTAGAACAAAATTTGAGAACTTTTAAGGTAACCATTTTTTTAATTTAAATTTTTAATTTTCTTTTAGTACTGTATGAACGAGTGATATGCTTTTCTTAGAAAAAATAATAATAATAAAATTGTTTTTCTTCTAAAATTATTTGTCTACAAATTGTGGTTATGATACCACCCATACTTCAATTCAATATATGCTCATAAATGTATGTTTCAACAAGCAGTTACAGTATTTTACATTTGTGTTAAAATCATAATCATATATATGTATTTTCTTTTTTTTTTTAAATAAAGCCAAGTGGAGATTATTAATTTTGTTGAATATTATTATTTAAAAAAAGACTATGCAGGAGTCATACACTAATTTTGTTGTATTGTTCCTACTCCAAGAAGGCATATTTTTAAATGTTAAAAGACAATTAACCCTCCACATTTTCTATCATACAATTTTATCTTTGGTTTCATCCAAAAGACATCATAACAATAGAAATATTGTCTTCTGTCTCATTTTTAGATGACCAACAACTTTCTATTGCATTCTTAGAAGGTATCATTACATATTGAATATTGTCGCCGCTCATGATGTATTTAGCCATGTATAATTTAAAAATTGAAAAATAAATAAATAAATAAGTTAATAGAACACAGAAAAGGTTTGATTACATTTAAAGAAAGAAAATATAGATTTTCTTAATTAGGAAAAGAGAAGATTGTTTGATAACTATTTTGCTTTTATTTTTTATTTTTATTTTTAAATATTAAGTTTAGAAGTATTTTTGCTTTGTTATACAAAAGTTTTAAAAGGTTAGTTTTTTGTTATTCATAATTTTTTTTAGTACAACATGTGAGGGTGACCGATTGGAACATGAAAATGTTTGTGTCTTGAGTAAGGAGAAAATGAAAATGTTTGTGTTTTGAGTAAGCAGAAAATGAAAATCGGTAATGTGATGGGGAGCAGGTTTTGAGCAATGGAAGGTACAAGAGTGTGATCCACAGGGCTGTGACCAACAGCGTCATGGAACGAGTATCCATGGTAATGTTCTATGCACCCAACGACGACACGGTGATCAAGCCAATTGAGGAGCTGACCGACGAACACCATCCTCCCTTGTACACAAGCTTCACTTACAGAGAGTTCATGCAACGCTTCCACGCCCAGGAAGGAAGAAGAAGAAGAGTCAAGGAAACTTTTGAGCTTCCCCACACCCCA

mRNA sequence

AGGTTCCAAATGGCGTGCGATTCTCAACCTCCTTCCCTGTCTCCTTCCTTTGTTCTCCCGGAGGACATAAGGCCACGTCTCCAACAAGTGTCACACGGCATCTCTGTTCCGGTCATCGACTTGGCCGCCGATGATCAATCCCTTGTGGTTGGAAAAGTATCGGAAGCCTGCCAGCAGTACGGCTTCTTCCAGGTCATCAATCACGGAATCCCGCAAGCCTTGTGCGACAGAGTACTGGACACAGTCGCCCAATTCTTCCACCTCCCTCCCCAACACAGGAACCAATACGTCACCACCGACCACAAGAAGAAGGTTAAAATCTTCAATTACTACGCCAACGTGGAGGGGCGGAGGGTCAACATGTGGAGCGAAACCTTCACGCTTCCCTGGAATCCCGATCCCGTGGAGACGGAGTATCTGGACGTGGTGCCCGAAGAGCCAGCCGAATACAGGGAAGTTTACGGGGAGTACGCGAGGGAGATGGGAAGCCTAATGGAGAGGCTTCTGGGGTTGATGTCGTTGGGGCTGGGCCTGGAGGAGGGCGCGCTCAAGAGGCGCATCGGGGCCAAGCCGGAGCTGTACTCGCAGGCCAACTACTACCCGCCCTGTCCGGACCCAGAGCTCACCATGGGCCTTGGGGCCCATACTGATATTGTAGCCATTGCGGTGCTGTTGCAGAACCGAGCCCCCAGTGGGCTCCATATCATGAAAGATGACCACTGGGTCTCCGTCGATCCTATTCCCAATTCACTTGTCGTCAATTTGGCTGATCAAGTCCAGCAGGTTTTGAGCAATGGAAGGTACAAGAGTGTGATCCACAGGGCTGTGACCAACAGCGTCATGGAACGAGTATCCATGGTAATGTTCTATGCACCCAACGACGACACGGTGATCAAGCCAATTGAGGAGCTGACCGACGAACACCATCCTCCCTTGTACACAAGCTTCACTTACAGAGAGTTCATGCAACGCTTCCACGCCCAGGAAGGAAGAAGAAGAAGAGTCAAGGAAACTTTTGAGCTTCCCCACACCCCA

Coding sequence (CDS)

AGGTTCCAAATGGCGTGCGATTCTCAACCTCCTTCCCTGTCTCCTTCCTTTGTTCTCCCGGAGGACATAAGGCCACGTCTCCAACAAGTGTCACACGGCATCTCTGTTCCGGTCATCGACTTGGCCGCCGATGATCAATCCCTTGTGGTTGGAAAAGTATCGGAAGCCTGCCAGCAGTACGGCTTCTTCCAGGTCATCAATCACGGAATCCCGCAAGCCTTGTGCGACAGAGTACTGGACACAGTCGCCCAATTCTTCCACCTCCCTCCCCAACACAGGAACCAATACGTCACCACCGACCACAAGAAGAAGGTTAAAATCTTCAATTACTACGCCAACGTGGAGGGGCGGAGGGTCAACATGTGGAGCGAAACCTTCACGCTTCCCTGGAATCCCGATCCCGTGGAGACGGAGTATCTGGACGTGGTGCCCGAAGAGCCAGCCGAATACAGGGAAGTTTACGGGGAGTACGCGAGGGAGATGGGAAGCCTAATGGAGAGGCTTCTGGGGTTGATGTCGTTGGGGCTGGGCCTGGAGGAGGGCGCGCTCAAGAGGCGCATCGGGGCCAAGCCGGAGCTGTACTCGCAGGCCAACTACTACCCGCCCTGTCCGGACCCAGAGCTCACCATGGGCCTTGGGGCCCATACTGATATTGTAGCCATTGCGGTGCTGTTGCAGAACCGAGCCCCCAGTGGGCTCCATATCATGAAAGATGACCACTGGGTCTCCGTCGATCCTATTCCCAATTCACTTGTCGTCAATTTGGCTGATCAAGTCCAGCAGGTTTTGAGCAATGGAAGGTACAAGAGTGTGATCCACAGGGCTGTGACCAACAGCGTCATGGAACGAGTATCCATGGTAATGTTCTATGCACCCAACGACGACACGGTGATCAAGCCAATTGAGGAGCTGACCGACGAACACCATCCTCCCTTGTACACAAGCTTCACTTACAGAGAGTTCATGCAACGCTTCCACGCCCAGGAAGGAAGAAGAAGAAGAGTCAAGGAAACTTTTGAGCTTCCCCACACCCCA

Protein sequence

RFQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHTP

Homology

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BLAST of MS000921 vs. NCBI nr
Match: XP_022132267.1 (flavanone 3-dioxygenase 2-like [Momordica charantia])

HSP 1 Score: 698.4 bits (1801), Expect = 3.2e-197
Identity = 342/345 (99.13%), Postives = 343/345 (99.42%), Query Frame = 0


Query: 1   RFQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQY 60
           RFQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQY
Sbjct: 4   RFQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQY 63

Query: 61  GFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVN 120
           GFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVN
Sbjct: 64  GFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVN 123

Query: 121 MWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEE 180
           MWSETFTLPWNPDPVETEYLDVVPEEPA YREVYGEYAREMGSLMERLLGLMSLGLGLEE
Sbjct: 124 MWSETFTLPWNPDPVETEYLDVVPEEPAVYREVYGEYAREMGSLMERLLGLMSLGLGLEE 183

Query: 181 GALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDH 240
           GALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQN+APSGLHIMKDDH
Sbjct: 184 GALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNQAPSGLHIMKDDH 243

Query: 241 WVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKP 300
           WVSVDPIPNSLVVNLADQV QVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKP
Sbjct: 244 WVSVDPIPNSLVVNLADQV-QVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKP 303

Query: 301 IEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHTP 346
           IEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHTP
Sbjct: 304 IEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHTP 347

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BLAST of MS000921 vs. NCBI nr
Match: KAG6598240.1 (Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029218.1 Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 525.4 bits (1352), Expect = 3.7e-145
Identity = 262/344 (76.16%), Postives = 294/344 (85.47%), Query Frame = 0


Query: 4   MACDSQPPSL--SPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQYG 63
           MA DS PPSL  S S+VLP+ +RP LQ VSH IS+P IDL   DQSL+V +VSEACQ +G
Sbjct: 1   MAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHTISLPTIDLNLHDQSLLVHQVSEACQSFG 60

Query: 64  FFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA-NVEGRRVN 123
           FFQVINHGIPQ LCD VLDT  QFFHLPPQ RNQYVTTDH KKVKIFNYYA +VEGR++N
Sbjct: 61  FFQVINHGIPQRLCDAVLDTTIQFFHLPPQQRNQYVTTDHTKKVKIFNYYAKDVEGRKIN 120

Query: 124 MWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEE 183
           +WSETFTLPW  DPV  E+++ VP+ P +YREVYGEYAREMG LMER+LGLMSLGLGL+E
Sbjct: 121 LWSETFTLPW--DPVGQEFVNDVPQNPPQYREVYGEYAREMGRLMERVLGLMSLGLGLDE 180

Query: 184 GALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDH 243
           GALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ + P+GL IMKD  
Sbjct: 181 GALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQEPAGLQIMKDQQ 240

Query: 244 WVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKP 303
           WVS+ P PNSLVVN+ADQ+ QVLSNGRYKSVIHRAVTNS M R+S+VMFYAPND+TVIKP
Sbjct: 241 WVSLLPRPNSLVVNVADQI-QVLSNGRYKSVIHRAVTNSAMTRLSLVMFYAPNDETVIKP 300

Query: 304 IEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
           IEELTDEHHPPLY +FTY++FM +F AQEG  RRVKETF+L +T
Sbjct: 301 IEELTDEHHPPLYKTFTYKQFMHQFRAQEGTIRRVKETFQLTNT 341

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BLAST of MS000921 vs. NCBI nr
Match: XP_022962245.1 (protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita moschata])

HSP 1 Score: 520.0 bits (1338), Expect = 1.6e-143
Identity = 260/346 (75.14%), Postives = 292/346 (84.39%), Query Frame = 0


Query: 2   FQMACDSQPPSL--SPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQ 61
           F+MA DS PPSL  S S+VLP+ +RP LQ VSH IS+P IDL   DQSL+V +VSEACQ 
Sbjct: 4   FKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHTISLPTIDLNLHDQSLLVHQVSEACQS 63

Query: 62  YGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA-NVEGRR 121
           +GFFQV NHGIPQ LCD VLDT  QFFHLPPQ RNQYVTTDH KKVKIFNYYA +VEGR+
Sbjct: 64  FGFFQVTNHGIPQRLCDAVLDTTIQFFHLPPQQRNQYVTTDHTKKVKIFNYYAKDVEGRK 123

Query: 122 VNMWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGL 181
           +N+WSETFTLPW  DPV  E+L  VP+ P +YREVYGEYAREMG LMER+LGLMSLGLGL
Sbjct: 124 INLWSETFTLPW--DPVGQEFLSDVPQNPPQYREVYGEYAREMGRLMERVLGLMSLGLGL 183

Query: 182 EEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKD 241
           +EGALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ +  +GL IMKD
Sbjct: 184 DEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQDAAGLQIMKD 243

Query: 242 DHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVI 301
             WVS+ P PNSLVVN+ADQ+ QVLSNGRYKSVIHRAVTNS   R+S+VMFYAPND+TVI
Sbjct: 244 QQWVSLLPRPNSLVVNVADQI-QVLSNGRYKSVIHRAVTNSARRRLSLVMFYAPNDETVI 303

Query: 302 KPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
           KPIEELTDEHHPPLY +FTY++FM +F AQEG  RRVKETF+L ++
Sbjct: 304 KPIEELTDEHHPPLYKTFTYKQFMHQFRAQEGTIRRVKETFQLTNS 346

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BLAST of MS000921 vs. NCBI nr
Match: XP_023545451.1 (protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.1 bits (1333), Expect = 5.9e-143
Identity = 257/346 (74.28%), Postives = 292/346 (84.39%), Query Frame = 0


Query: 2   FQMACDSQPPSL--SPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQ 61
           F+MA DS PPSL  S S+VLP+ +RP LQ VSH IS+P IDL   DQSL+V +VSEACQ 
Sbjct: 4   FKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEACQS 63

Query: 62  YGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA-NVEGRR 121
           +GFFQVINHGIPQ LCD VLDT  QFFHLPP+ RNQYVTTDH KKVKIFNYYA +VEGR+
Sbjct: 64  FGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRK 123

Query: 122 VNMWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGL 181
           +N+WSETFTLPW  DP   E+L+ VP+ P +YREVYGEY+REMG LMER+LGLMSLGLGL
Sbjct: 124 INLWSETFTLPW--DPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGL 183

Query: 182 EEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKD 241
           +EGALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ++  +GL IMKD
Sbjct: 184 DEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKD 243

Query: 242 DHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVI 301
             WVS+ P PNSLVVN+ADQ+ QVLSNGRYKSVIHRAVTN    R+S+VMFYAPND+TVI
Sbjct: 244 QQWVSLLPHPNSLVVNVADQI-QVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI 303

Query: 302 KPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
           KPIEELTDEHHPPLY +FTY++FM  F AQEG  RRVKETF+L ++
Sbjct: 304 KPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNS 346

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BLAST of MS000921 vs. NCBI nr
Match: XP_022996769.1 (protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita maxima])

HSP 1 Score: 507.3 bits (1305), Expect = 1.0e-139
Identity = 254/353 (71.95%), Postives = 291/353 (82.44%), Query Frame = 0


Query: 2   FQMACDSQPPSL---------SPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGK 61
           F+MA DS PPSL         S S+VLP+ +RP LQ+VSH ISVP IDL   D+SL+V +
Sbjct: 4   FKMAFDSLPPSLSSSSSSSSSSSSYVLPDHMRPDLQRVSHTISVPTIDLDLHDRSLLVHQ 63

Query: 62  VSEACQQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA 121
           VSEAC  +GFFQV NHGIPQ LCD VLDT  QFFHLPP+ RNQYVTTDH KKVKIFNYYA
Sbjct: 64  VSEACHSFGFFQVTNHGIPQHLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYA 123

Query: 122 -NVEGRRVNMWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGL 181
            +VEGR++N+WSETFTLPW  DP+  ++L+ VP+ P +YREVYGEYAREMG LMER+LGL
Sbjct: 124 KDVEGRKINLWSETFTLPW--DPMGQKFLNDVPQNPPQYREVYGEYAREMGRLMERVLGL 183

Query: 182 MSLGLGLEEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPS 241
           MSLGLGL+EGALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ +  +
Sbjct: 184 MSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQEAA 243

Query: 242 GLHIMKDDHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYA 301
           GL IMKD  WVS+ P PNSLVVN+ADQ+ QVLSNGRYKSVIHRAVTN    R+S+VMFYA
Sbjct: 244 GLQIMKDHQWVSLLPHPNSLVVNVADQI-QVLSNGRYKSVIHRAVTNRARGRLSLVMFYA 303

Query: 302 PNDDTVIKPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
           PND+TV+KPIEELTDEHHPPLY SFTY++FM  F AQEG  RRVKE+F+L ++
Sbjct: 304 PNDETVMKPIEELTDEHHPPLYKSFTYKQFMHEFRAQEGTIRRVKESFQLTNS 353

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BLAST of MS000921 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-58
Identity = 121/323 (37.46%), Postives = 189/323 (58.51%), Query Frame = 0


Query: 17  FVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQYGFFQVINHGIPQALCD 76
           +V PE  RPRL  V     +PV+DLA+ D++ VV  V +AC+ +GFFQV+NHGI  AL  
Sbjct: 24  YVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGIDAALIA 83

Query: 77  RVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWNPDPVE 136
            V++   +FF LP + + +  + D  KK+++   + NV    V+ W +   L   P    
Sbjct: 84  SVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHCYP---L 143

Query: 137 TEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKPELYSQ 196
            +++   P  P  ++E+ G Y  E+  L  RL   +S  LGLE G ++  +G + E +  
Sbjct: 144 HQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLG-EQEQHMA 203

Query: 197 ANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSLVVNLA 256
            NYYP CP+PELT GL AHTD  A+ +LL +   +GL ++ D  W++V+P P +LV+N+ 
Sbjct: 204 VNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINIG 263

Query: 257 DQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHHPPLYTSF 316
           DQ+ Q LSNG+Y+SV HRAV NS  ER+S+  F  P +   + P ++L  +  P +Y ++
Sbjct: 264 DQL-QALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRNY 323

Query: 317 TYREFMQRFHAQEGRRRRVKETF 340
           TY E+ ++F ++   +    E F
Sbjct: 324 TYDEYYKKFWSRNLDQEHCLELF 340

[Close]

BLAST of MS000921 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 5.2e-57
Identity = 120/331 (36.25%), Postives = 196/331 (59.21%), Query Frame = 0


Query: 12  SLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQYGFFQVINHGIP 71
           +L  ++V P   RPRL +VS     P+IDL++ D+S ++ ++ +AC ++GFFQVINHG+ 
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 72  QALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWN 131
           + + D ++    +FF +  + + +  + D  K  ++   + NV+   VN W +   L   
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133

Query: 132 PDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKP 191
           P     +Y++  P  P  ++E+  +Y+RE+  +  ++  L+S  LGLE+  +K+ +G + 
Sbjct: 134 P---IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQG 193

Query: 192 ELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSL 251
           + +   NYYPPCP+PELT GL AHTD  A+ +LLQ+    GL I+ D  W +V+P P++ 
Sbjct: 194 Q-HMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 253

Query: 252 VVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVI---KPIEELTDEH 311
           V+N+ DQ+ Q LSNG YKSV HRAVTN+   R+S+  F  P D  V+   KP+ E  D+ 
Sbjct: 254 VINIGDQL-QALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDE 313

Query: 312 HPPLYTSFTYREFMQRFHAQEGRRRRVKETF 340
             P+Y  FTY E+ ++F ++   +    E F
Sbjct: 314 TKPVYKDFTYAEYYKKFWSRNLDQEHCLENF 338

[Close]

BLAST of MS000921 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.0e-56
Identity = 127/334 (38.02%), Postives = 194/334 (58.08%), Query Frame = 0


Query: 16  SFVLPEDIRPRLQQV-SHGISVPVIDLA---ADDQSLVVGKVSEACQQYGFFQVINHGIP 75
           ++V P   RP L +V S G S+P+IDL      +++++V +++ AC  YGFFQ+ NHG+P
Sbjct: 23  NYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNHGVP 82

Query: 76  QALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWN 135
               +++     +FFH P   R ++ + D  K  ++   + NV   +V  W +   L  +
Sbjct: 83  DTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKVLNWRDFLRL--H 142

Query: 136 PDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKP 195
             P+E ++++  P  P  +REV  EYA  + +L+ RLL  +S  LGLE   +   +G K 
Sbjct: 143 CFPIE-DFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILG-KH 202

Query: 196 ELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSL 255
             +   NYYPPCP+PELT GL  H D   I VLLQ++  SGL + KDD WV+V PIPN+ 
Sbjct: 203 AQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQV-SGLQVFKDDKWVAVSPIPNTF 262

Query: 256 VVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHHP- 315
           +VN+ DQ+ QV+SN +YKSV+HRAV N+  ER+S+  FY P+ D VI P  EL +E    
Sbjct: 263 IVNIGDQM-QVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSL 322

Query: 316 PLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
            +Y ++ + E+  +F  +        + F+ P T
Sbjct: 323 AIYRTYPFVEYWDKFWNRSLATASCLDAFKAPTT 349

[Close]

BLAST of MS000921 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.4e-56
Identity = 121/316 (38.29%), Postives = 190/316 (60.13%), Query Frame = 0


Query: 16  SFVLPEDIRPRLQQV-SHGISVPVI---DLAADDQSLVVGKVSEACQQYGFFQVINHGIP 75
           ++V P   RP++ +V + G S+P+I   DL   +++ ++ + + AC   GFFQ+ NHG+P
Sbjct: 21  NYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGVP 80

Query: 76  QALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWN 135
           +    ++++   +FF      R ++ + D KK  ++   + NV   +V+ W +   L  +
Sbjct: 81  EETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDFLRL--H 140

Query: 136 PDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKP 195
             P+E ++++  P  P  +REV  EYA  + +L+  LL  +S  LGL +  +   IG K 
Sbjct: 141 CYPIE-DFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG-KH 200

Query: 196 ELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSL 255
             +   NYYP CP PELT GL  H D   I VLLQ+   SGL + KD  W++V+P+PN+ 
Sbjct: 201 GQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEV-SGLQVFKDGKWIAVNPVPNTF 260

Query: 256 VVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHH-- 315
           +VNL DQ+ QV+SN +YKSV+HRAV NS MER+S+  FY P++D VI P +EL +E    
Sbjct: 261 IVNLGDQM-QVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 320

Query: 316 PPLYTSFTYREFMQRF 326
           P +Y +FTY E+ ++F
Sbjct: 321 PAIYRNFTYAEYFEKF 329

[Close]

BLAST of MS000921 vs. ExPASy Swiss-Prot
Match: Q96330 (Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana OX=3702 GN=FLS1 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.5e-52
Identity = 107/292 (36.64%), Postives = 166/292 (56.85%), Query Frame = 0


Query: 35  SVPVIDLAADDQSLVVGKVSEACQQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRN 94
           ++PV+DL+  D+  V   V +A +++G FQV+NHGIP  L  R+ D   +FF LP   + 
Sbjct: 42  AIPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKE 101

Query: 95  QYVTTDHKKKVKIFNYYANVEGRRVNMW-SETFTLPWNPDPVETEYLDVVPEEPAEYREV 154
                +  K ++ +      +      W    F   W P  V   +    P+ P EYREV
Sbjct: 102 SVAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFW---PKNPPEYREV 161

Query: 155 YGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAK-PELYSQANYYPPCPDPELTMGL 214
             EYA  +  L E LLG++S GLGL+  ALK  +G +  E   + NYYPPCP P+L +G+
Sbjct: 162 NEEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGV 221

Query: 215 GAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVI 274
            AHTD+  I +L+ N  P GL + KDDHW   + IP++++V++ DQ+ + LSNGRYK+V+
Sbjct: 222 PAHTDLSGITLLVPNEVP-GLQVFKDDHWFDAEYIPSAVIVHIGDQILR-LSNGRYKNVL 281

Query: 275 HRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHHPPLYTSFTYREFMQR 325
           HR   +    R+S  +F  P  + ++ P+ ELT + +PP +  F ++++  R
Sbjct: 282 HRTTVDKEKTRMSWPVFLEPPREKIVGPLPELTGDDNPPKFKPFAFKDYSYR 328

[Close]

BLAST of MS000921 vs. ExPASy TrEMBL
Match: A0A6J1BTD6 (flavanone 3-dioxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111005163 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.5e-197
Identity = 342/345 (99.13%), Postives = 343/345 (99.42%), Query Frame = 0


Query: 1   RFQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQY 60
           RFQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQY
Sbjct: 4   RFQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQY 63

Query: 61  GFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVN 120
           GFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVN
Sbjct: 64  GFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVN 123

Query: 121 MWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEE 180
           MWSETFTLPWNPDPVETEYLDVVPEEPA YREVYGEYAREMGSLMERLLGLMSLGLGLEE
Sbjct: 124 MWSETFTLPWNPDPVETEYLDVVPEEPAVYREVYGEYAREMGSLMERLLGLMSLGLGLEE 183

Query: 181 GALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDH 240
           GALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQN+APSGLHIMKDDH
Sbjct: 184 GALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNQAPSGLHIMKDDH 243

Query: 241 WVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKP 300
           WVSVDPIPNSLVVNLADQV QVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKP
Sbjct: 244 WVSVDPIPNSLVVNLADQV-QVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKP 303

Query: 301 IEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHTP 346
           IEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHTP
Sbjct: 304 IEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHTP 347

[Close]

BLAST of MS000921 vs. ExPASy TrEMBL
Match: A0A6J1HEH8 (protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita moschata OX=3662 GN=LOC111462753 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 7.5e-144
Identity = 260/346 (75.14%), Postives = 292/346 (84.39%), Query Frame = 0


Query: 2   FQMACDSQPPSL--SPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQ 61
           F+MA DS PPSL  S S+VLP+ +RP LQ VSH IS+P IDL   DQSL+V +VSEACQ 
Sbjct: 4   FKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHTISLPTIDLNLHDQSLLVHQVSEACQS 63

Query: 62  YGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA-NVEGRR 121
           +GFFQV NHGIPQ LCD VLDT  QFFHLPPQ RNQYVTTDH KKVKIFNYYA +VEGR+
Sbjct: 64  FGFFQVTNHGIPQRLCDAVLDTTIQFFHLPPQQRNQYVTTDHTKKVKIFNYYAKDVEGRK 123

Query: 122 VNMWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGL 181
           +N+WSETFTLPW  DPV  E+L  VP+ P +YREVYGEYAREMG LMER+LGLMSLGLGL
Sbjct: 124 INLWSETFTLPW--DPVGQEFLSDVPQNPPQYREVYGEYAREMGRLMERVLGLMSLGLGL 183

Query: 182 EEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKD 241
           +EGALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ +  +GL IMKD
Sbjct: 184 DEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQDAAGLQIMKD 243

Query: 242 DHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVI 301
             WVS+ P PNSLVVN+ADQ+ QVLSNGRYKSVIHRAVTNS   R+S+VMFYAPND+TVI
Sbjct: 244 QQWVSLLPRPNSLVVNVADQI-QVLSNGRYKSVIHRAVTNSARRRLSLVMFYAPNDETVI 303

Query: 302 KPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
           KPIEELTDEHHPPLY +FTY++FM +F AQEG  RRVKETF+L ++
Sbjct: 304 KPIEELTDEHHPPLYKTFTYKQFMHQFRAQEGTIRRVKETFQLTNS 346

[Close]

BLAST of MS000921 vs. ExPASy TrEMBL
Match: A0A6J1K9L9 (protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491903 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 5.1e-140
Identity = 254/353 (71.95%), Postives = 291/353 (82.44%), Query Frame = 0


Query: 2   FQMACDSQPPSL---------SPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGK 61
           F+MA DS PPSL         S S+VLP+ +RP LQ+VSH ISVP IDL   D+SL+V +
Sbjct: 4   FKMAFDSLPPSLSSSSSSSSSSSSYVLPDHMRPDLQRVSHTISVPTIDLDLHDRSLLVHQ 63

Query: 62  VSEACQQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA 121
           VSEAC  +GFFQV NHGIPQ LCD VLDT  QFFHLPP+ RNQYVTTDH KKVKIFNYYA
Sbjct: 64  VSEACHSFGFFQVTNHGIPQHLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYA 123

Query: 122 -NVEGRRVNMWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGL 181
            +VEGR++N+WSETFTLPW  DP+  ++L+ VP+ P +YREVYGEYAREMG LMER+LGL
Sbjct: 124 KDVEGRKINLWSETFTLPW--DPMGQKFLNDVPQNPPQYREVYGEYAREMGRLMERVLGL 183

Query: 182 MSLGLGLEEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPS 241
           MSLGLGL+EGALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ +  +
Sbjct: 184 MSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQEAA 243

Query: 242 GLHIMKDDHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYA 301
           GL IMKD  WVS+ P PNSLVVN+ADQ+ QVLSNGRYKSVIHRAVTN    R+S+VMFYA
Sbjct: 244 GLQIMKDHQWVSLLPHPNSLVVNVADQI-QVLSNGRYKSVIHRAVTNRARGRLSLVMFYA 303

Query: 302 PNDDTVIKPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
           PND+TV+KPIEELTDEHHPPLY SFTY++FM  F AQEG  RRVKE+F+L ++
Sbjct: 304 PNDETVMKPIEELTDEHHPPLYKSFTYKQFMHEFRAQEGTIRRVKESFQLTNS 353

[Close]

BLAST of MS000921 vs. ExPASy TrEMBL
Match: A0A7N2R1R3 (Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 3.1e-113
Identity = 201/338 (59.47%), Postives = 258/338 (76.33%), Query Frame = 0


Query: 7   DSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQYGFFQVI 66
           ++ P SLSP+F+LPE+ RP L +V    S+P IDL   D   +V K+S+AC++YGFFQ+I
Sbjct: 10  NNTPLSLSPNFILPEENRPILSEVFSSASIPTIDLNNHDIDDLVSKISQACEKYGFFQII 69

Query: 67  NHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGR-RVNMWSET 126
           NHG+P+ LC RVL  V +FF LPP+ R Q++TTDH+K VK+FNYY  VEG+ +V MW ET
Sbjct: 70  NHGVPKELCQRVLAVVTEFFQLPPEERAQFLTTDHRKLVKVFNYYQKVEGQGKVLMWGET 129

Query: 127 FTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKR 186
           FT PW+P    T +L   P  P +YREV+ EYARE+G LM+RLL LMS GLGLE+  LKR
Sbjct: 130 FTHPWHPTENFTHFL---PTNPPQYREVFTEYAREIGGLMDRLLSLMSQGLGLEKDCLKR 189

Query: 187 RIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVD 246
           ++G +P  Y+QANYYPPCPDPELTMGL AH DI A+ VLLQ    SGL ++ D  WV V+
Sbjct: 190 KLGERPATYTQANYYPPCPDPELTMGLPAHNDISALVVLLQMEGVSGLQVVIDGKWVPVE 249

Query: 247 PIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKPIEELT 306
           P+P++ VVN+ADQ+ QVLSNGRYKSV+HRAVTN+ + R+S+ MFYAPN+DTVI PIE+L 
Sbjct: 250 PVPDAFVVNIADQM-QVLSNGRYKSVLHRAVTNNRLARLSLPMFYAPNNDTVIGPIEDLI 309

Query: 307 DEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPH 344
           DE HPP+Y +++Y+E+M  F+ QEG RRRVKE FEL H
Sbjct: 310 DEVHPPMYRNYSYKEYMDEFYRQEGARRRVKEAFELQH 343

[Close]

BLAST of MS000921 vs. ExPASy TrEMBL
Match: A0A7N2LA75 (Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 3.2e-110
Identity = 201/347 (57.93%), Postives = 262/347 (75.50%), Query Frame = 0


Query: 2   FQMACDSQPPSLSPSFVLPEDIRPRLQQVSHGISVPVIDL------AADDQSLVVGKVSE 61
           FQ+A ++ P SLSP F+LPE  RP L +V    S+P+IDL           S +V K+S+
Sbjct: 7   FQLA-NNTPLSLSPDFILPEFKRPILSKVFPSDSIPIIDLNDHEIDEGQGSSPLVSKISQ 66

Query: 62  ACQQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVE 121
           AC++YGFFQ+INHG+P+ LC +VL  V +FF LP + R QY+T DH K+VK+FNYY   E
Sbjct: 67  ACEEYGFFQIINHGVPKELCQKVLAVVTEFFQLPHEERAQYLTKDHTKQVKVFNYYQKYE 126

Query: 122 G-RRVNMWSETFTLPWNPDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSL 181
           G  +V MWSETF+ PW+  P+E ++  ++P  P +YREV+ EYA+E+G+LM+RLL L+S 
Sbjct: 127 GLGKVIMWSETFSHPWH--PIE-DFTHLLPTNPPQYREVFAEYAKEIGALMDRLLSLISQ 186

Query: 182 GLGLEEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLH 241
           GLGLE+  LK++IG +  LYSQANYYPPCPDPELTMG+ AH DIVA+ VLLQ+   +GL 
Sbjct: 187 GLGLEKDCLKKKIGERASLYSQANYYPPCPDPELTMGIPAHNDIVALTVLLQSEGVTGLQ 246

Query: 242 IMKDDHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPND 301
           ++K++ WV VDP+P+S VVNLADQ+ QVLSNGRYKSV HRAVTN  + RVS+ MFYAPN+
Sbjct: 247 VIKEEKWVPVDPVPHSFVVNLADQI-QVLSNGRYKSVDHRAVTNKWLPRVSLAMFYAPNN 306

Query: 302 DTVIKPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFEL 342
           D VI PIE+L DE HPP Y +++Y+EFM  F+ QEG+RRRVKE FEL
Sbjct: 307 DIVIGPIEDLIDEEHPPKYRNYSYKEFMDEFYRQEGKRRRVKEAFEL 348

[Close]

BLAST of MS000921 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 223.0 bits (567), Expect = 3.7e-58
Identity = 120/331 (36.25%), Postives = 196/331 (59.21%), Query Frame = 0


Query: 12  SLSPSFVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEACQQYGFFQVINHGIP 71
           +L  ++V P   RPRL +VS     P+IDL++ D+S ++ ++ +AC ++GFFQVINHG+ 
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVN 73

Query: 72  QALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWN 131
           + + D ++    +FF +  + + +  + D  K  ++   + NV+   VN W +   L   
Sbjct: 74  KQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNNWRDYLRLHCY 133

Query: 132 PDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKP 191
           P     +Y++  P  P  ++E+  +Y+RE+  +  ++  L+S  LGLE+  +K+ +G + 
Sbjct: 134 P---IHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQG 193

Query: 192 ELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSL 251
           + +   NYYPPCP+PELT GL AHTD  A+ +LLQ+    GL I+ D  W +V+P P++ 
Sbjct: 194 Q-HMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 253

Query: 252 VVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVI---KPIEELTDEH 311
           V+N+ DQ+ Q LSNG YKSV HRAVTN+   R+S+  F  P D  V+   KP+ E  D+ 
Sbjct: 254 VINIGDQL-QALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDE 313

Query: 312 HPPLYTSFTYREFMQRFHAQEGRRRRVKETF 340
             P+Y  FTY E+ ++F ++   +    E F
Sbjct: 314 TKPVYKDFTYAEYYKKFWSRNLDQEHCLENF 338

[Close]

BLAST of MS000921 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.4e-57
Identity = 127/334 (38.02%), Postives = 194/334 (58.08%), Query Frame = 0


Query: 16  SFVLPEDIRPRLQQV-SHGISVPVIDLA---ADDQSLVVGKVSEACQQYGFFQVINHGIP 75
           ++V P   RP L +V S G S+P+IDL      +++++V +++ AC  YGFFQ+ NHG+P
Sbjct: 23  NYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNHGVP 82

Query: 76  QALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWN 135
               +++     +FFH P   R ++ + D  K  ++   + NV   +V  W +   L  +
Sbjct: 83  DTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKVLNWRDFLRL--H 142

Query: 136 PDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKP 195
             P+E ++++  P  P  +REV  EYA  + +L+ RLL  +S  LGLE   +   +G K 
Sbjct: 143 CFPIE-DFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDHISNILG-KH 202

Query: 196 ELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSL 255
             +   NYYPPCP+PELT GL  H D   I VLLQ++  SGL + KDD WV+V PIPN+ 
Sbjct: 203 AQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQV-SGLQVFKDDKWVAVSPIPNTF 262

Query: 256 VVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHHP- 315
           +VN+ DQ+ QV+SN +YKSV+HRAV N+  ER+S+  FY P+ D VI P  EL +E    
Sbjct: 263 IVNIGDQM-QVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSL 322

Query: 316 PLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 345
            +Y ++ + E+  +F  +        + F+ P T
Sbjct: 323 AIYRTYPFVEYWDKFWNRSLATASCLDAFKAPTT 349

[Close]

BLAST of MS000921 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 3.1e-57
Identity = 121/316 (38.29%), Postives = 190/316 (60.13%), Query Frame = 0


Query: 16  SFVLPEDIRPRLQQV-SHGISVPVI---DLAADDQSLVVGKVSEACQQYGFFQVINHGIP 75
           ++V P   RP++ +V + G S+P+I   DL   +++ ++ + + AC   GFFQ+ NHG+P
Sbjct: 21  NYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGVP 80

Query: 76  QALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYANVEGRRVNMWSETFTLPWN 135
           +    ++++   +FF      R ++ + D KK  ++   + NV   +V+ W +   L  +
Sbjct: 81  EETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDFLRL--H 140

Query: 136 PDPVETEYLDVVPEEPAEYREVYGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAKP 195
             P+E ++++  P  P  +REV  EYA  + +L+  LL  +S  LGL +  +   IG K 
Sbjct: 141 CYPIE-DFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG-KH 200

Query: 196 ELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSL 255
             +   NYYP CP PELT GL  H D   I VLLQ+   SGL + KD  W++V+P+PN+ 
Sbjct: 201 GQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEV-SGLQVFKDGKWIAVNPVPNTF 260

Query: 256 VVNLADQVQQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHH-- 315
           +VNL DQ+ QV+SN +YKSV+HRAV NS MER+S+  FY P++D VI P +EL +E    
Sbjct: 261 IVNLGDQM-QVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 320

Query: 316 PPLYTSFTYREFMQRF 326
           P +Y +FTY E+ ++F
Sbjct: 321 PAIYRNFTYAEYFEKF 329

[Close]

BLAST of MS000921 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 206.1 bits (523), Expect = 4.6e-53
Identity = 107/292 (36.64%), Postives = 166/292 (56.85%), Query Frame = 0


Query: 35  SVPVIDLAADDQSLVVGKVSEACQQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRN 94
           ++PV+DL+  D+  V   V +A +++G FQV+NHGIP  L  R+ D   +FF LP   + 
Sbjct: 42  AIPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKE 101

Query: 95  QYVTTDHKKKVKIFNYYANVEGRRVNMW-SETFTLPWNPDPVETEYLDVVPEEPAEYREV 154
                +  K ++ +      +      W    F   W P  V   +    P+ P EYREV
Sbjct: 102 SVAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFW---PKNPPEYREV 161

Query: 155 YGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAK-PELYSQANYYPPCPDPELTMGL 214
             EYA  +  L E LLG++S GLGL+  ALK  +G +  E   + NYYPPCP P+L +G+
Sbjct: 162 NEEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGV 221

Query: 215 GAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVI 274
            AHTD+  I +L+ N  P GL + KDDHW   + IP++++V++ DQ+ + LSNGRYK+V+
Sbjct: 222 PAHTDLSGITLLVPNEVP-GLQVFKDDHWFDAEYIPSAVIVHIGDQILR-LSNGRYKNVL 281

Query: 275 HRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHHPPLYTSFTYREFMQR 325
           HR   +    R+S  +F  P  + ++ P+ ELT + +PP +  F ++++  R
Sbjct: 282 HRTTVDKEKTRMSWPVFLEPPREKIVGPLPELTGDDNPPKFKPFAFKDYSYR 328

[Close]

BLAST of MS000921 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 206.1 bits (523), Expect = 4.6e-53
Identity = 107/292 (36.64%), Postives = 166/292 (56.85%), Query Frame = 0


Query: 35  SVPVIDLAADDQSLVVGKVSEACQQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRN 94
           ++PV+DL+  D+  V   V +A +++G FQV+NHGIP  L  R+ D   +FF LP   + 
Sbjct: 42  AIPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKE 101

Query: 95  QYVTTDHKKKVKIFNYYANVEGRRVNMW-SETFTLPWNPDPVETEYLDVVPEEPAEYREV 154
                +  K ++ +      +      W    F   W P  V   +    P+ P EYREV
Sbjct: 102 SVAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFW---PKNPPEYREV 161

Query: 155 YGEYAREMGSLMERLLGLMSLGLGLEEGALKRRIGAK-PELYSQANYYPPCPDPELTMGL 214
             EYA  +  L E LLG++S GLGL+  ALK  +G +  E   + NYYPPCP P+L +G+
Sbjct: 162 NEEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGV 221

Query: 215 GAHTDIVAIAVLLQNRAPSGLHIMKDDHWVSVDPIPNSLVVNLADQVQQVLSNGRYKSVI 274
            AHTD+  I +L+ N  P GL + KDDHW   + IP++++V++ DQ+ + LSNGRYK+V+
Sbjct: 222 PAHTDLSGITLLVPNEVP-GLQVFKDDHWFDAEYIPSAVIVHIGDQILR-LSNGRYKNVL 281

Query: 275 HRAVTNSVMERVSMVMFYAPNDDTVIKPIEELTDEHHPPLYTSFTYREFMQR 325
           HR   +    R+S  +F  P  + ++ P+ ELT + +PP +  F ++++  R
Sbjct: 282 HRTTVDKEKTRMSWPVFLEPPREKIVGPLPELTGDDNPPKFKPFAFKDYSYR 328

The following BLAST results are available for this feature:

BLAST of MS000921 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132267.1	3.2e-197	99.13	flavanone 3-dioxygenase 2-like [Momordica charantia]	[more]
KAG6598240.1	3.7e-145	76.16	Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022962245.1	1.6e-143	75.14	protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita moschata]	[more]
XP_023545451.1	5.9e-143	74.28	protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022996769.1	1.0e-139	71.95	protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita maxima]	[more]

BLAST of MS000921 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W2X5	2.1e-58	37.46	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]
Q9FLV0	5.2e-57	36.25	Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S...	[more]
Q9ZSA8	2.0e-56	38.02	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1	[more]
Q9ZSA7	4.4e-56	38.29	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q96330	6.5e-52	36.64	Flavonol synthase/flavanone 3-hydroxylase OS=Arabidopsis thaliana OX=3702 GN=FLS...	[more]

BLAST of MS000921 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTD6	1.5e-197	99.13	flavanone 3-dioxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111005163 PE...	[more]
A0A6J1HEH8	7.5e-144	75.14	protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1K9L9	5.1e-140	71.95	protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita maxima OX=3661 GN=LOC1114919...	[more]
A0A7N2R1R3	3.1e-113	59.47	Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]
A0A7N2LA75	3.2e-110	57.93	Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of MS000921 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24530.1	3.7e-58	36.25	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10500.1	1.4e-57	38.02	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	3.1e-57	38.29	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G08640.1	4.6e-53	36.64	flavonol synthase 1	[more]
AT5G08640.2	4.6e-53	36.64	flavonol synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 197..292 e-value: 6.3E-23 score: 81.2
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 36..132 e-value: 1.1E-20 score: 74.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..331 e-value: 3.1E-89 score: 301.5
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 6..341
None	No IPR available	PANTHER	PTHR47990:SF124	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE	coord: 6..341
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 5..328
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 190..292 score: 12.2497

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000921.1	MS000921.1	mRNA