MS000887 (gene) Bitter gourd (TR) v1

Overview


Name	MS000887
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	polygalacturonase 1 beta-like protein 3
Location	scaffold36: 72294 .. 74174 (-)
RNA-Seq Expression	MS000887
Synteny	MS000887

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGAGTTTTTGTCCCCTCCTCTCCTCATACGCCCTTGTAATCTTTACATCATTTTGTTTTTTCTGCCTCTGCCAAAACAGGTTTCCGGCGCCGGCGAGGCGCCGACGGAGGATAACCCCTTTACTCCGAAAGCTTTCCTAATTCGTTACTGGAATAAAGAGGTGAGTAATGACCTGCCAAAGCCCACTTTTCTGTTCTCCAAGGTCTCCCCCTTGACCGCGGTGCAGTCGGCAGCCTTCGCCAAACTCGCAGCCCAAAATGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCAAACCTGCTCTGCTTCCCCGATTTGTCCCCTAGTTTGGCCAAGCACGACAAGGACTCCAGCTTCGCCGTCTATGCCAATAAGAACTTTACCAATTACGGCTCCCAAAGCCCTGGCGGAATCGACTCCTTCAAGAACTATTCACAGAGCGAAAACGTCGTGGTCGATTCCTTCAGGCGCTACAGCCGCGAGTCGTTGGGCCACACGGACCAATTTACGAGCTACGGCACCGACACCAACGTACCGGACCAGAGCTTCAACACTTATGGCACCAAGGCCATCGGAGGCAACGGCAAGTTCACAAACTACGAACAATCCACGAATGTGCCCAATCTCCGATTTACGTCCTATTCCAGCGAGGCCACAGGAAGATCCCAATCGTTCAAGAGCTACAGCGAGAACGCGAACGCGGGAGATCAGTCGTTTGCCAACTATGGGAAACGTGGAAATGGGGGTCCGAACGAGTTCACGGGCTACGGGACCAACGCGAACGTAGTAGGGTCAGGGTTTTCGAGCTACGGGGCAGAGGGCAATGGCGCTAACGACACGTTCACCAATTACGGCAACGAGCAGAACAACCCACAAAATAACTTCAGGAGCTACGGCGAAGGTGGGAATGGAGCGATTGAAAGCTTCGCTAATTACAGGGACAAGGCGAATGTGGGGGATGATTCCTTCCAGTCCTACTCCAAGGACTCGAATTCGGCCGAGGTCAATTTCGCAAACTACGGGAAATCCTTCAACGAGGGGACGGACAAATTTAGCGGTTATGGTAAGAATGGAAATGGGCAAAGCGTGGGATTCAAGACCTATGGCGTGAATACGACGTTCAAAGATTACGCGAAAGAGGGAATTAGATTTGCGAAATACAGCAATGTGAGCTCTGGGTCTGGAGGTGGGAAAATGTGGGTGAACAGGTGGGTGGAGCCGGGTAAGTTCTTCCGGGAGTCGATGTTGAGGAAGGGGGCGGTGATGCCGATGCCGGACATCAGAGATAAAATGCCGAGAAGGTCGTTTTTGCCCCGATCTATTCTGTCGAAATTACCCTTTTCGAGTTCAAAACTGGGGGCGATGAAGCACATATTCCACGCCGGGGACAACTCGTCGATGGAGACCATGATGAGGGAGACAGTGACGGAGTGCGAGAGGGCGGCGAGCGCGGGGGAGACGAAGCGGTGCGTGGGGTCAATGGAGGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACTTGGCGGTCCGGACGACGGAGAACACGGAGGGATCCAAGGGGAAGATAATGATAGGGGCGGTGAAGGGGATAAACGGAGGGAGGGTAACGGAGTCGGTGTCTTGCCATCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCCGTGCCCAAGGTCCGGGTGTACGAAGCGGATTTGCTGGATCCGACCTCTAAAGCAAAGATCAACCGCGGAGTTGCCATCTGCCACTTGGACACCTCCGCCTGGAGTCCCGCTCACGGAGCCTTCATAGCTCTGGCTTCCGCCCCGGGTCGGGTCGAGGTGTGCCACTGGATATTCGAGAACGACATGACCTGGACCCCGGCCGAC

mRNA sequence

CTTGAGTTTTTGTCCCCTCCTCTCCTCATACGCCCTTGTAATCTTTACATCATTTTGTTTTTTCTGCCTCTGCCAAAACAGGTTTCCGGCGCCGGCGAGGCGCCGACGGAGGATAACCCCTTTACTCCGAAAGCTTTCCTAATTCGTTACTGGAATAAAGAGGTGAGTAATGACCTGCCAAAGCCCACTTTTCTGTTCTCCAAGGTCTCCCCCTTGACCGCGGTGCAGTCGGCAGCCTTCGCCAAACTCGCAGCCCAAAATGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCAAACCTGCTCTGCTTCCCCGATTTGTCCCCTAGTTTGGCCAAGCACGACAAGGACTCCAGCTTCGCCGTCTATGCCAATAAGAACTTTACCAATTACGGCTCCCAAAGCCCTGGCGGAATCGACTCCTTCAAGAACTATTCACAGAGCGAAAACGTCGTGGTCGATTCCTTCAGGCGCTACAGCCGCGAGTCGTTGGGCCACACGGACCAATTTACGAGCTACGGCACCGACACCAACGTACCGGACCAGAGCTTCAACACTTATGGCACCAAGGCCATCGGAGGCAACGGCAAGTTCACAAACTACGAACAATCCACGAATGTGCCCAATCTCCGATTTACGTCCTATTCCAGCGAGGCCACAGGAAGATCCCAATCGTTCAAGAGCTACAGCGAGAACGCGAACGCGGGAGATCAGTCGTTTGCCAACTATGGGAAACGTGGAAATGGGGGTCCGAACGAGTTCACGGGCTACGGGACCAACGCGAACGTAGTAGGGTCAGGGTTTTCGAGCTACGGGGCAGAGGGCAATGGCGCTAACGACACGTTCACCAATTACGGCAACGAGCAGAACAACCCACAAAATAACTTCAGGAGCTACGGCGAAGGTGGGAATGGAGCGATTGAAAGCTTCGCTAATTACAGGGACAAGGCGAATGTGGGGGATGATTCCTTCCAGTCCTACTCCAAGGACTCGAATTCGGCCGAGGTCAATTTCGCAAACTACGGGAAATCCTTCAACGAGGGGACGGACAAATTTAGCGGTTATGGTAAGAATGGAAATGGGCAAAGCGTGGGATTCAAGACCTATGGCGTGAATACGACGTTCAAAGATTACGCGAAAGAGGGAATTAGATTTGCGAAATACAGCAATGTGAGCTCTGGGTCTGGAGGTGGGAAAATGTGGGTGAACAGGTGGGTGGAGCCGGGTAAGTTCTTCCGGGAGTCGATGTTGAGGAAGGGGGCGGTGATGCCGATGCCGGACATCAGAGATAAAATGCCGAGAAGGTCGTTTTTGCCCCGATCTATTCTGTCGAAATTACCCTTTTCGAGTTCAAAACTGGGGGCGATGAAGCACATATTCCACGCCGGGGACAACTCGTCGATGGAGACCATGATGAGGGAGACAGTGACGGAGTGCGAGAGGGCGGCGAGCGCGGGGGAGACGAAGCGGTGCGTGGGGTCAATGGAGGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACTTGGCGGTCCGGACGACGGAGAACACGGAGGGATCCAAGGGGAAGATAATGATAGGGGCGGTGAAGGGGATAAACGGAGGGAGGGTAACGGAGTCGGTGTCTTGCCATCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCCGTGCCCAAGGTCCGGGTGTACGAAGCGGATTTGCTGGATCCGACCTCTAAAGCAAAGATCAACCGCGGAGTTGCCATCTGCCACTTGGACACCTCCGCCTGGAGTCCCGCTCACGGAGCCTTCATAGCTCTGGCTTCCGCCCCGGGTCGGGTCGAGGTGTGCCACTGGATATTCGAGAACGACATGACCTGGACCCCGGCCGAC

Coding sequence (CDS)

CTTGAGTTTTTGTCCCCTCCTCTCCTCATACGCCCTTGTAATCTTTACATCATTTTGTTTTTTCTGCCTCTGCCAAAACAGGTTTCCGGCGCCGGCGAGGCGCCGACGGAGGATAACCCCTTTACTCCGAAAGCTTTCCTAATTCGTTACTGGAATAAAGAGGTGAGTAATGACCTGCCAAAGCCCACTTTTCTGTTCTCCAAGGTCTCCCCCTTGACCGCGGTGCAGTCGGCAGCCTTCGCCAAACTCGCAGCCCAAAATGCACTCTCCTCCCGCCTCCCCGAGTTCTGCTCCGCCGCAAACCTGCTCTGCTTCCCCGATTTGTCCCCTAGTTTGGCCAAGCACGACAAGGACTCCAGCTTCGCCGTCTATGCCAATAAGAACTTTACCAATTACGGCTCCCAAAGCCCTGGCGGAATCGACTCCTTCAAGAACTATTCACAGAGCGAAAACGTCGTGGTCGATTCCTTCAGGCGCTACAGCCGCGAGTCGTTGGGCCACACGGACCAATTTACGAGCTACGGCACCGACACCAACGTACCGGACCAGAGCTTCAACACTTATGGCACCAAGGCCATCGGAGGCAACGGCAAGTTCACAAACTACGAACAATCCACGAATGTGCCCAATCTCCGATTTACGTCCTATTCCAGCGAGGCCACAGGAAGATCCCAATCGTTCAAGAGCTACAGCGAGAACGCGAACGCGGGAGATCAGTCGTTTGCCAACTATGGGAAACGTGGAAATGGGGGTCCGAACGAGTTCACGGGCTACGGGACCAACGCGAACGTAGTAGGGTCAGGGTTTTCGAGCTACGGGGCAGAGGGCAATGGCGCTAACGACACGTTCACCAATTACGGCAACGAGCAGAACAACCCACAAAATAACTTCAGGAGCTACGGCGAAGGTGGGAATGGAGCGATTGAAAGCTTCGCTAATTACAGGGACAAGGCGAATGTGGGGGATGATTCCTTCCAGTCCTACTCCAAGGACTCGAATTCGGCCGAGGTCAATTTCGCAAACTACGGGAAATCCTTCAACGAGGGGACGGACAAATTTAGCGGTTATGGTAAGAATGGAAATGGGCAAAGCGTGGGATTCAAGACCTATGGCGTGAATACGACGTTCAAAGATTACGCGAAAGAGGGAATTAGATTTGCGAAATACAGCAATGTGAGCTCTGGGTCTGGAGGTGGGAAAATGTGGGTGAACAGGTGGGTGGAGCCGGGTAAGTTCTTCCGGGAGTCGATGTTGAGGAAGGGGGCGGTGATGCCGATGCCGGACATCAGAGATAAAATGCCGAGAAGGTCGTTTTTGCCCCGATCTATTCTGTCGAAATTACCCTTTTCGAGTTCAAAACTGGGGGCGATGAAGCACATATTCCACGCCGGGGACAACTCGTCGATGGAGACCATGATGAGGGAGACAGTGACGGAGTGCGAGAGGGCGGCGAGCGCGGGGGAGACGAAGCGGTGCGTGGGGTCAATGGAGGACATGATAGACTTCGCGACGTCGGTGCTGGGGCGGAACTTGGCGGTCCGGACGACGGAGAACACGGAGGGATCCAAGGGGAAGATAATGATAGGGGCGGTGAAGGGGATAAACGGAGGGAGGGTAACGGAGTCGGTGTCTTGCCATCAGAGCCTGTTCCCTTACCTACTCTACTACTGCCACTCCGTGCCCAAGGTCCGGGTGTACGAAGCGGATTTGCTGGATCCGACCTCTAAAGCAAAGATCAACCGCGGAGTTGCCATCTGCCACTTGGACACCTCCGCCTGGAGTCCCGCTCACGGAGCCTTCATAGCTCTGGCTTCCGCCCCGGGTCGGGTCGAGGTGTGCCACTGGATATTCGAGAACGACATGACCTGGACCCCGGCCGAC

Protein sequence

LEFLSPPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYGKRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWTPAD

Homology

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BLAST of MS000887 vs. NCBI nr
Match: XP_022159130.1 (polygalacturonase 1 beta-like protein 3 [Momordica charantia])

HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 606/622 (97.43%), Postives = 609/622 (97.91%), Query Frame = 0


Query: 6   PPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL 65
           P  L+ P  LY+ +FF      VSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL
Sbjct: 2   PAPLVVP--LYLFIFFFS-AINVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL 61

Query: 66  FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA 125
           FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA
Sbjct: 62  FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA 121

Query: 126 NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF 185
           NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF
Sbjct: 122 NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF 181

Query: 186 NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG 245
           NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG
Sbjct: 182 NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG 241

Query: 246 KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN 305
           KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN
Sbjct: 242 KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN 301

Query: 306 GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV 365
           GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV
Sbjct: 302 GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV 361

Query: 366 GFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMP 425
           GFKTYGVNTTFKDYAKEGIRFAKYSNV SGSGGGKMWVNRWVEPGKFFRESMLRKGAVMP
Sbjct: 362 GFKTYGVNTTFKDYAKEGIRFAKYSNVGSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMP 421

Query: 426 MPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAAS 485
           MPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAAS
Sbjct: 422 MPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAAS 481

Query: 486 AGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSC 545
           AGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSC
Sbjct: 482 AGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSC 541

Query: 546 HQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALAS 605
           HQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALAS
Sbjct: 542 HQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALAS 601

Query: 606 APGRVEVCHWIFENDMTWTPAD 628
           APGRVEVCHWIFENDMTWTPAD
Sbjct: 602 APGRVEVCHWIFENDMTWTPAD 620

[Close]

BLAST of MS000887 vs. NCBI nr
Match: XP_023546090.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1047.3 bits (2707), Expect = 5.1e-302
Identity = 536/629 (85.21%), Postives = 569/629 (90.46%), Query Frame = 0


Query: 4   LSPPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPT 63
           L  PLL+    LY+ +F L  P  V  A +   EDNPFTPKAFL RYWNKEV NDLPKPT
Sbjct: 4   LPAPLLVL---LYLFIFLLS-PLNVPAAAD---EDNPFTPKAFLNRYWNKEVRNDLPKPT 63

Query: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAV 123
           FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHD  S+FAV
Sbjct: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAV 123

Query: 124 YANKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQ 183
           Y+NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTDQF++YGTDTNVPDQ
Sbjct: 124 YSNKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQ 183

Query: 184 SFNTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFAN 243
           SFNTY +KA+GGNG FT YEQS NVPNLRFT+YSS+ATGRSQSFKSYSENANAGDQSF N
Sbjct: 184 SFNTYASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVN 243

Query: 244 YGKRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEG 303
           YGKRGNGGPNEFTGYGTNANVVGSGFSSYGA GNGANDTFTNYGNEQNNPQNNFRSYG+G
Sbjct: 244 YGKRGNGGPNEFTGYGTNANVVGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDG 303

Query: 304 GNGAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQ 363
           GNGAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFANYGKSFNEGTDKFSGYG+NGNGQ
Sbjct: 304 GNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQ 363

Query: 364 SVGFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESML 423
           SVGFKTYGVNTTFKDYAK+GI FAKYSNVS  SGG KM V+     RWVEPGKFFRESML
Sbjct: 364 SVGFKTYGVNTTFKDYAKQGISFAKYSNVS--SGGAKMSVSESLAKRWVEPGKFFRESML 423

Query: 424 RKGAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVT 483
           RKG VM MPDIRDKMP+RSFLPRSILSKLPFSSSKL  MK IFHAGDNSSMETMM ET+ 
Sbjct: 424 RKGTVMAMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLR 483

Query: 484 ECERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGR 543
           +CER  SAGETKRCVGS+EDMIDFATSVLGRN+ VRTTENT+GSKG IMIGAVKGI GG+
Sbjct: 484 DCERTPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGK 543

Query: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHG 603
           VTESVSCHQSLFPYLLYYCHSVPKVRVYEA+LLDPTSKA IN GVAICHLDTSAWSPAHG
Sbjct: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHG 603

Query: 604 AFIALASAPGRVEVCHWIFENDMTWTPAD 628
           AFIAL SAPGRVEVCHWIFENDMTW+ AD
Sbjct: 604 AFIALGSAPGRVEVCHWIFENDMTWSVAD 623

[Close]

BLAST of MS000887 vs. NCBI nr
Match: XP_022961767.1 (polygalacturonase 1 beta-like protein 3 [Cucurbita moschata])

HSP 1 Score: 1047.0 bits (2706), Expect = 6.7e-302
Identity = 535/629 (85.06%), Postives = 569/629 (90.46%), Query Frame = 0


Query: 4   LSPPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPT 63
           L  PLL+    LY+ +F L  P  V  A +   EDNPFTPKAFL RYWNKEV NDLPKPT
Sbjct: 4   LPAPLLVL---LYLFIFLLS-PLNVPAAAD---EDNPFTPKAFLNRYWNKEVRNDLPKPT 63

Query: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAV 123
           FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHD  S+FAV
Sbjct: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAV 123

Query: 124 YANKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQ 183
           Y+NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTDQF++YGTDTNVPDQ
Sbjct: 124 YSNKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQ 183

Query: 184 SFNTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFAN 243
           SFNTY +KA+GGNG FT YEQS NVPNLRFT+YSS+ATGRSQSFKSYSENANAGDQSF N
Sbjct: 184 SFNTYASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVN 243

Query: 244 YGKRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEG 303
           YGKRGNGGPNEFTGYGTNANV+GSGFSSYGA GNGANDTFTNYGNEQNNPQNNFRSYG+G
Sbjct: 244 YGKRGNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDG 303

Query: 304 GNGAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQ 363
           GNGAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFANYGKSFNEGTDKFSGYG+NGNGQ
Sbjct: 304 GNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQ 363

Query: 364 SVGFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESML 423
           SVGFKTYGVNTTFKDYAK+GI FAKYSNVS  SGG KM V+     RWVEPGKFFRESML
Sbjct: 364 SVGFKTYGVNTTFKDYAKQGISFAKYSNVS--SGGAKMSVSESLAKRWVEPGKFFRESML 423

Query: 424 RKGAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVT 483
           RKG VM MPDIRDKMP+RSFLPRSILSKLPFSSSKL  MK IFHAGDNSSMETMM ET+ 
Sbjct: 424 RKGTVMAMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLR 483

Query: 484 ECERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGR 543
           +CER  SAGETKRCVGS+EDMIDFATSVLGRN+ VRTTENT+GSKG IMIGAVKGI GG+
Sbjct: 484 DCERTPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGK 543

Query: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHG 603
           VTESVSCHQSLFPYLLYYCHSVPKVRVYEA+LLDPTSKA IN GVAICHLDTSAWSPAHG
Sbjct: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHG 603

Query: 604 AFIALASAPGRVEVCHWIFENDMTWTPAD 628
           AFIAL SAPGRVEVCHWIFENDMTW+ AD
Sbjct: 604 AFIALGSAPGRVEVCHWIFENDMTWSVAD 623

[Close]

BLAST of MS000887 vs. NCBI nr
Match: KAG6598207.1 (Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.9e-301
Identity = 534/629 (84.90%), Postives = 569/629 (90.46%), Query Frame = 0


Query: 4   LSPPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPT 63
           L  PLL+    LY+ +F L  P  V  A +   EDNPFTPKAFL RYWNKEV NDLPKPT
Sbjct: 4   LPAPLLVL---LYLFIFLLS-PLNVPAAAD---EDNPFTPKAFLNRYWNKEVRNDLPKPT 63

Query: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAV 123
           FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKH+  S+FAV
Sbjct: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHESTSNFAV 123

Query: 124 YANKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQ 183
           Y+NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTDQF++YGTDTNVPDQ
Sbjct: 124 YSNKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSAYGTDTNVPDQ 183

Query: 184 SFNTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFAN 243
           SFNTY +KA+GGNG FT YEQS NVPNLRFT+YSS+ATGRSQSFKSYSENANAGDQSF N
Sbjct: 184 SFNTYASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVN 243

Query: 244 YGKRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEG 303
           YGKRGNGGPNEFTGYGTNANV+GSGFSSYGA GNGANDTFTNYGNEQNNPQNNFRSYG+G
Sbjct: 244 YGKRGNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDG 303

Query: 304 GNGAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQ 363
           GNGAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFANYGKSFNEGTDKFSGYG+NGNGQ
Sbjct: 304 GNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQ 363

Query: 364 SVGFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESML 423
           SVGFKTYGVNTTFKDYAK+GI FAKYSNVS  SGG KM V+     RWVEPGKFFRESML
Sbjct: 364 SVGFKTYGVNTTFKDYAKQGISFAKYSNVS--SGGAKMSVSESLAKRWVEPGKFFRESML 423

Query: 424 RKGAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVT 483
           RKG VM MPDIRDKMP+RSFLPRSILSKLPFSSSKL  MK IFHAGDNSSMETMM ET+ 
Sbjct: 424 RKGTVMAMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLR 483

Query: 484 ECERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGR 543
           +CER  SAGETKRCVGS+EDMIDFATSVLGRN+ VRTTENT+GSKG IMIGAVKGI GG+
Sbjct: 484 DCERTPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGK 543

Query: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHG 603
           VTESVSCHQSLFPYLLYYCHSVPKVRVYEA+LLDPTSKA IN GVAICHLDTSAWSPAHG
Sbjct: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHG 603

Query: 604 AFIALASAPGRVEVCHWIFENDMTWTPAD 628
           AFIAL SAPGRVEVCHWIFENDMTW+ AD
Sbjct: 604 AFIALGSAPGRVEVCHWIFENDMTWSVAD 623

[Close]

BLAST of MS000887 vs. NCBI nr
Match: KAG7029192.1 (Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1044.6 bits (2700), Expect = 3.3e-301
Identity = 534/629 (84.90%), Postives = 568/629 (90.30%), Query Frame = 0


Query: 4   LSPPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPT 63
           L  PLL+    LY+ +F L  P  V  A +   EDNPFTPKAFL RYWNKEV NDLPKPT
Sbjct: 4   LPAPLLVL---LYLFIFLLS-PLNVPAAAD---EDNPFTPKAFLNRYWNKEVRNDLPKPT 63

Query: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAV 123
           FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHD  S+FAV
Sbjct: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAV 123

Query: 124 YANKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQ 183
           Y+NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTDQF++YGTDTNVPDQ
Sbjct: 124 YSNKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSAYGTDTNVPDQ 183

Query: 184 SFNTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFAN 243
           SFNTY +KA+GGNG FT YEQS NVPNLRFT+YSS+ATGRSQSFKSYSENANAGDQSF N
Sbjct: 184 SFNTYASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVN 243

Query: 244 YGKRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEG 303
           YGKRGNGGPNEFTGYGTNANV+GSGFSSYGA GNGANDTFTNYGNEQNNPQNNFRSYG+G
Sbjct: 244 YGKRGNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDG 303

Query: 304 GNGAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQ 363
           GNGAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFANYGKSFNEGTDKFSGYG+NGNGQ
Sbjct: 304 GNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQ 363

Query: 364 SVGFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESML 423
           SVGFKTYGVNTTFKDYAK+GI FAKYSNVS  SGG KM V+     RWVEPGKFFRESML
Sbjct: 364 SVGFKTYGVNTTFKDYAKQGISFAKYSNVS--SGGAKMSVSESLAKRWVEPGKFFRESML 423

Query: 424 RKGAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVT 483
           RKG VM MPDIRDKMP+RSFLPRSILSKLPFSSSKL  MK IFHAGDNSSMETMM ET+ 
Sbjct: 424 RKGTVMAMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLR 483

Query: 484 ECERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGR 543
           +CER  SAGETKRCVGS+EDMIDFATSVLGRN+ VRTTENT+GSKG IMIGAVKGI GG+
Sbjct: 484 DCERTPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGK 543

Query: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHG 603
           VTESVSCHQSLFPYLLYYCH VPKVRVYEA+LLDPTSKA IN GVAICHLDTSAWSPAHG
Sbjct: 544 VTESVSCHQSLFPYLLYYCHFVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHG 603

Query: 604 AFIALASAPGRVEVCHWIFENDMTWTPAD 628
           AFIAL SAPGRVEVCHWIFENDMTW+ AD
Sbjct: 604 AFIALGSAPGRVEVCHWIFENDMTWSVAD 623

[Close]

BLAST of MS000887 vs. ExPASy Swiss-Prot
Match: P92990 (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 PE=2 SV=2)

HSP 1 Score: 798.9 bits (2062), Expect = 4.1e-230
Identity = 390/599 (65.11%), Postives = 473/599 (78.96%), Query Frame = 0


Query: 32  GEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAAQNALSS 91
           G    E+NPFTPKA L+RYWNKE+    P+  FL SK SPL AV SA F+KLAA N+L +
Sbjct: 28  GGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPT 87

Query: 92  RLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKNYSQSEN 151
           R P+FCSAANL CFPDL  SL KHD D  F+VY  KNFTNYG+   GG DSFKNYS+  N
Sbjct: 88  RFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGN 147

Query: 152 VVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQSTNVPNL 211
           VV DSFRRYSR + GH D+FT YG ++NV ++ FN+YGT   GG G FTNY+ + N P  
Sbjct: 148 VVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTS 207

Query: 212 RFTSYSSEATGRSQSFKSYSENANAGD-QSFANYGKRGNGGPNEFTGYGTNANVVGSGFS 271
           RFT+YS    GRSQ+FK+Y+  ANAG+ QSF +YGK GNG PNEFT YG ++NV+GSGFS
Sbjct: 208 RFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFS 267

Query: 272 SYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDSFQSYSK 331
           +YG  GN ANDTFT+YG++ N PQNNF +YG  GN A+++FANYRDKANVGDDSF SY+K
Sbjct: 268 NYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAK 327

Query: 332 DSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGIRFAKY- 391
           DSNS +VNF NYG+SFN G++ F+GYGK   G  + FKTY  N+TFKDYAK+G+ FAKY 
Sbjct: 328 DSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKYN 387

Query: 392 -SNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSILSKLP 451
            S  ++ + G    VN+W+EPGKFFRES L++G V+PMPDI+DKMP+RSFLPRSI++KLP
Sbjct: 388 VSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLP 447

Query: 452 FSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFATSVLG 511
           FS+SKLG +K IFHA +NS+M  ++ + VTECER  S GETKRCVGS EDMIDFATSVLG
Sbjct: 448 FSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLG 507

Query: 512 RNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVRVYEA 571
           R++ +RTTEN  GSK K++IG V GINGG++T++VSCHQSL+PYLLYYCHSVPKVRVYEA
Sbjct: 508 RSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEA 567

Query: 572 DLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWTPAD 628
           DLL+  SK KIN G+AICH+DTS+W P+HGAF+AL S PGR+EVCHWIFENDM W  AD
Sbjct: 568 DLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

[Close]

BLAST of MS000887 vs. ExPASy Swiss-Prot
Match: P92982 (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 PE=2 SV=2)

HSP 1 Score: 783.5 bits (2022), Expect = 1.8e-225
Identity = 384/603 (63.68%), Postives = 468/603 (77.61%), Query Frame = 0


Query: 26  KQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAA 85
           ++++  G +P E NPFTPKA L+RYWNK++ +  P   FL SK SPLTAV SA FAKLA+
Sbjct: 24  ERLTNGGFSP-EINPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLAS 83

Query: 86  QNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKN 145
           +NAL  RLP+FCSAANL CFPDL P+L KH  D  F+VY  +NFTNYG+  PGG DSFK 
Sbjct: 84  ENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRNFTNYGTIHPGGADSFKK 143

Query: 146 YSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQS 205
           YSQ+ N V DSFRRYSR++ GH D+FT Y  ++NV ++ FN+YGT A GG+G F NY+  
Sbjct: 144 YSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSG 203

Query: 206 TNVPNLRFTSYSSEATGRSQSFKSYSENANAG-DQSFANYGKRGNGGPNEFTGYGTNANV 265
            N P  RF +YS E  GR+Q+FK+Y+  AN+G  QSF +YGK GNG PNEF+ YG ++NV
Sbjct: 204 VNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNV 263

Query: 266 VGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDS 325
           +GS FS+YG  GN ANDTF  YG + N PQNNF+SYG  GN A+++F NYRD ANVGDDS
Sbjct: 264 IGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDS 323

Query: 326 FQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGI 385
           F SY+K+SN  +V+F NYGKS N G++ FSGYGK   G  + FKTY  N+TFKDY K G+
Sbjct: 324 FSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKIDFKTYTQNSTFKDYTKTGV 383

Query: 386 RFAKYSNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSIL 445
            FAKY+   S  GGGK  VN+WVEPGKFFRESML++G ++ MPDI+DKMP+RSFLPRSI+
Sbjct: 384 EFAKYNR--SSLGGGKT-VNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIV 443

Query: 446 SKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFAT 505
           SKLPFS+SK+  +K +FHA DNS+ME ++ + V ECER  +  ETKRCVGS EDMIDFAT
Sbjct: 444 SKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFAT 503

Query: 506 SVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVR 565
           SVLGR++ +RTTE+  GSK K+MIG V GINGGRVT+SVSCHQSL+PYLLYYCHSVPKVR
Sbjct: 504 SVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVR 563

Query: 566 VYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWT 625
           VYE+DLLDP SKAKIN G+AICH+DTSAW   HGAF+ L S PG++EVCHWIFENDM W 
Sbjct: 564 VYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWA 622

Query: 626 PAD 628
            AD
Sbjct: 624 IAD 622

[Close]

BLAST of MS000887 vs. ExPASy Swiss-Prot
Match: Q40161 (Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 GN=GP1 PE=1 SV=1)

HSP 1 Score: 775.0 bits (2000), Expect = 6.4e-223
Identity = 391/626 (62.46%), Postives = 472/626 (75.40%), Query Frame = 0


Query: 12  PCNLYIILFFLP-LPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVS 71
           PC L ++LF LP     V G GE+    NPFTPK +LIRYW K++SNDLPKP FL +K S
Sbjct: 9   PCILLLLLFSLPSFNVVVGGDGES---GNPFTPKGYLIRYWKKQISNDLPKPWFLLNKAS 68

Query: 72  PLTAVQSAAFAKLAA-QNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNF 131
           PL A Q A + KL A QNAL+++L  FCS+ANL+C PDLSPSL KH  D  FA Y++KNF
Sbjct: 69  PLNAAQYATYTKLVADQNALTTQLHTFCSSANLMCAPDLSPSLEKHSGDIHFATYSDKNF 128

Query: 132 TNYGSQSPG-GIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTY 191
           TNYG+  PG G+++FKNYS+ EN+ V+SFRRY R S    ++F +Y +D NV DQSFN+Y
Sbjct: 129 TNYGTNEPGIGVNTFKNYSEGENIPVNSFRRYGRGS-PRDNKFDNYASDGNVIDQSFNSY 188

Query: 192 GTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYGKRG 251
            T   GG+GKFTNY  + N PNL FTSYS + TG  Q F  YS+ ANAGDQ F +YGK G
Sbjct: 189 STSTAGGSGKFTNYAANANDPNLHFTSYSDQGTGGVQKFTIYSQEANAGDQYFKSYGKNG 248

Query: 252 NGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAI 311
           NG   EF  YG + NV+GS F++YG   NG +  FT+YG   N P+N+F +YG GGNG  
Sbjct: 249 NGANGEFVSYGNDTNVIGSTFTNYGQTANGGDQKFTSYGFNGNVPENHFTNYGAGGNGPS 308

Query: 312 ESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFK 371
           E+F +YRD++NVGDD+F +Y KD+N  E NF NYG+SFNEGTD F+ YGK GN   + FK
Sbjct: 309 ETFNSYRDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGGNDPHINFK 368

Query: 372 TYGVNTTFKDYAKEGIRFAKYSNVSS-------GSGGGKMWVNRWVEPGKFFRESMLRKG 431
           TYGVN TFKDY K+   F+ Y N +S          GGK   NRWVEPGKFFRE ML+ G
Sbjct: 369 TYGVNNTFKDYVKDTATFSNYHNKTSQVLASLMEVNGGKKVNNRWVEPGKFFREKMLKSG 428

Query: 432 AVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECE 491
            +MPMPDI+DKMP+RSFLPR I SKLPFS+SK+  +K IFHAGD S +E M+ + ++ECE
Sbjct: 429 TIMPMPDIKDKMPKRSFLPRVIASKLPFSTSKIAELKKIFHAGDESQVEKMIGDALSECE 488

Query: 492 RAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTE 551
           RA SAGETKRCV S EDMIDFATSVLGRN+ VRTTE+T+GS G IMIG+VKGINGG+VT+
Sbjct: 489 RAPSAGETKRCVNSAEDMIDFATSVLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGKVTK 548

Query: 552 SVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFI 611
           SVSCHQ+L+PYLLYYCHSVPKVRVYEAD+LDP SK KIN GVAICH+DTS+W P+HGAF+
Sbjct: 549 SVSCHQTLYPYLLYYCHSVPKVRVYEADILDPNSKVKINHGVAICHVDTSSWGPSHGAFV 608

Query: 612 ALASAPGRVEVCHWIFENDMTWTPAD 628
           AL S PG++EVCHWIFENDMTW  AD
Sbjct: 609 ALGSGPGKIEVCHWIFENDMTWAIAD 630

[Close]

BLAST of MS000887 vs. ExPASy Swiss-Prot
Match: O80760 (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 PE=2 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 8.1e-218
Identity = 376/598 (62.88%), Postives = 453/598 (75.75%), Query Frame = 0


Query: 31  AGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAAQNALS 90
           AG     +NPFTPKA LIRYWN  ++ D PKP+F  SK SPLTAV S  FA LA+ +AL+
Sbjct: 30  AGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHALN 89

Query: 91  SRLPEFCSAANLLCFPDLSP-SLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKNYSQS 150
           +   +FCSAA L CFP+L+  SL KH  D +FA Y+ KNFTNYGS    G DSFKNYS  
Sbjct: 90  THHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSGADSFKNYSGG 149

Query: 151 ENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQSTNVP 210
           +N+ VDSFRRYSR S GH D FT+Y  + NV DQSF TY T   GG+G+FTNY    N P
Sbjct: 150 DNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEFTNYNTDANEP 209

Query: 211 NLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYGKRGNGGPNEFTGYGTNANVVGSGF 270
           N RFTSYS +A GRSQ+F +YSEN N G QSF +Y K GNG PNEF+GYGT +NVV +GF
Sbjct: 210 NGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYGTGSNVVNTGF 269

Query: 271 SSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDSFQSYS 330
           + YG   NGAND+FT+YG   N P N F+ YG+GGNGA+  F NYRD++N+G DSF SY+
Sbjct: 270 TKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSNIGVDSFSSYA 329

Query: 331 KDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGIRFAKY 390
           K+SN+ +VNF NYGKSFN G+D F+GYG++  G +V FKTYG   +FK Y K+G+ FA+Y
Sbjct: 330 KNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSFKTYGQGQSFKVYTKDGVVFARY 389

Query: 391 SNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSILSKLPF 450
           SN  S +G     VN+WVE GKFFRE+ML++G +M MPDI+DKMP+R+FLPR+I+  LPF
Sbjct: 390 SNNVSSNG---KTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPRNIVKNLPF 449

Query: 451 SSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFATSVLGR 510
           SSS +G +  +F AG+NSSM  ++   V+ECER AS GETKRCVGS EDMIDFATSVLGR
Sbjct: 450 SSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMIDFATSVLGR 509

Query: 511 NLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVRVYEAD 570
            + VRTTEN  GSK K++IG V GINGG VT +VSCHQSL+PYLLYYCHSVP+VRVYE D
Sbjct: 510 GVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVPRVRVYETD 569

Query: 571 LLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWTPAD 628
           LLDP S  KIN GVAICH+DTSAWSP+HGAF+AL S PG++EVCHWIFENDMTW   D
Sbjct: 570 LLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDMTWNIID 624

[Close]

BLAST of MS000887 vs. ExPASy Swiss-Prot
Match: P93217 (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 GN=GP2 PE=3 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.8e-217
Identity = 380/622 (61.09%), Postives = 458/622 (73.63%), Query Frame = 0


Query: 15  LYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTA 74
           + ++L F      +  A +     NPFTPK ++IRYWNK VSNDLPKP FL +K SPL A
Sbjct: 11  ILLVLLFSLSSFNIVVAKDGDESGNPFTPKGYVIRYWNKHVSNDLPKPWFLLNKASPLNA 70

Query: 75  VQSAAFAKLAA-QNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNFTNYG 134
            Q A + KL A QNALS+ L  FCS+ANL+C PDL PSL KH  D  F  Y NKNFTNYG
Sbjct: 71  AQYATYTKLVADQNALSTHLQSFCSSANLMCAPDLLPSLEKHTGDIHFTTYGNKNFTNYG 130

Query: 135 SQSPG-GIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKA 194
           +  PG G+++FKNY  SE+  V+SFRRY R S    ++F +Y  D NV DQSFN+Y T  
Sbjct: 131 TNEPGIGVNTFKNY--SEDASVNSFRRYGRGS-PRDNKFDNYAPDGNVIDQSFNSYSTNT 190

Query: 195 IGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYGKRGNGGP 254
            GG+G+FTNY  +TNVP+LRFT+YS + TG  Q FK+Y E  N+G QSF +YGK GNG  
Sbjct: 191 PGGSGQFTNYAPNTNVPDLRFTAYSDQGTGGEQEFKTYLEQGNSGGQSFKSYGKNGNGAD 250

Query: 255 NEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFA 314
           ++FT YG   NV  S F +YG   NG N  FT+Y    NNPQNNF++YG GGNG  E+F 
Sbjct: 251 SKFTSYGNETNVAASTFKNYGQNANGENQNFTSYSTNGNNPQNNFKNYGVGGNGPSETFT 310

Query: 315 NYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGV 374
           NYRD++NVGDD F +Y KD+N+ E NF NYG+SFNEGTD F  YGK GN   + FKTYGV
Sbjct: 311 NYRDESNVGDDKFSNYVKDANAGEANFTNYGQSFNEGTDVFITYGKGGNDPHINFKTYGV 370

Query: 375 NTTFKDYAKEGIRFAKYSNVSSGS-------GGGKMWVNRWVEPGKFFRESMLRKGAVMP 434
           N TFKDY K+   F+ Y N +S          GGK   NRW+EPGKFFRE ML+ G +MP
Sbjct: 371 NNTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGKKVNNRWIEPGKFFREKMLKSGTIMP 430

Query: 435 MPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAAS 494
           MPDI+DKMP+RSFLPR+I +KLPFS+SK+  +K IFHA ++S +  M+ + ++ECERA S
Sbjct: 431 MPDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDALSECERAPS 490

Query: 495 AGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSC 554
            GETK+CV S EDMIDFATSVLGRN+ VRTTENT GSKG IMIG++KGINGG+VT+SVSC
Sbjct: 491 PGETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSVSC 550

Query: 555 HQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALAS 614
           HQ+L+P LLYYCHSVPKVRVYEAD+LDP SKAKIN GVAICH+DTS+W P HGAFIAL S
Sbjct: 551 HQTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRHGAFIALGS 610

Query: 615 APGRVEVCHWIFENDMTWTPAD 628
            PG++EVCHWIFENDMTW  AD
Sbjct: 611 GPGKIEVCHWIFENDMTWATAD 629

[Close]

BLAST of MS000887 vs. ExPASy TrEMBL
Match: A0A6J1DXS8 (polygalacturonase 1 beta-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111025560 PE=4 SV=1)

HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 606/622 (97.43%), Postives = 609/622 (97.91%), Query Frame = 0


Query: 6   PPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL 65
           P  L+ P  LY+ +FF      VSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL
Sbjct: 2   PAPLVVP--LYLFIFFFS-AINVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL 61

Query: 66  FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA 125
           FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA
Sbjct: 62  FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA 121

Query: 126 NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF 185
           NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF
Sbjct: 122 NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF 181

Query: 186 NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG 245
           NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG
Sbjct: 182 NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG 241

Query: 246 KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN 305
           KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN
Sbjct: 242 KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN 301

Query: 306 GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV 365
           GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV
Sbjct: 302 GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV 361

Query: 366 GFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMP 425
           GFKTYGVNTTFKDYAKEGIRFAKYSNV SGSGGGKMWVNRWVEPGKFFRESMLRKGAVMP
Sbjct: 362 GFKTYGVNTTFKDYAKEGIRFAKYSNVGSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMP 421

Query: 426 MPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAAS 485
           MPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAAS
Sbjct: 422 MPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAAS 481

Query: 486 AGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSC 545
           AGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSC
Sbjct: 482 AGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSC 541

Query: 546 HQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALAS 605
           HQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALAS
Sbjct: 542 HQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALAS 601

Query: 606 APGRVEVCHWIFENDMTWTPAD 628
           APGRVEVCHWIFENDMTWTPAD
Sbjct: 602 APGRVEVCHWIFENDMTWTPAD 620

[Close]

BLAST of MS000887 vs. ExPASy TrEMBL
Match: A0A6J1HB03 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111462438 PE=4 SV=1)

HSP 1 Score: 1047.0 bits (2706), Expect = 3.2e-302
Identity = 535/629 (85.06%), Postives = 569/629 (90.46%), Query Frame = 0


Query: 4   LSPPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPT 63
           L  PLL+    LY+ +F L  P  V  A +   EDNPFTPKAFL RYWNKEV NDLPKPT
Sbjct: 4   LPAPLLVL---LYLFIFLLS-PLNVPAAAD---EDNPFTPKAFLNRYWNKEVRNDLPKPT 63

Query: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAV 123
           FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHD  S+FAV
Sbjct: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAV 123

Query: 124 YANKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQ 183
           Y+NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTDQF++YGTDTNVPDQ
Sbjct: 124 YSNKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQ 183

Query: 184 SFNTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFAN 243
           SFNTY +KA+GGNG FT YEQS NVPNLRFT+YSS+ATGRSQSFKSYSENANAGDQSF N
Sbjct: 184 SFNTYASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVN 243

Query: 244 YGKRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEG 303
           YGKRGNGGPNEFTGYGTNANV+GSGFSSYGA GNGANDTFTNYGNEQNNPQNNFRSYG+G
Sbjct: 244 YGKRGNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDG 303

Query: 304 GNGAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQ 363
           GNGAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFANYGKSFNEGTDKFSGYG+NGNGQ
Sbjct: 304 GNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQ 363

Query: 364 SVGFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESML 423
           SVGFKTYGVNTTFKDYAK+GI FAKYSNVS  SGG KM V+     RWVEPGKFFRESML
Sbjct: 364 SVGFKTYGVNTTFKDYAKQGISFAKYSNVS--SGGAKMSVSESLAKRWVEPGKFFRESML 423

Query: 424 RKGAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVT 483
           RKG VM MPDIRDKMP+RSFLPRSILSKLPFSSSKL  MK IFHAGDNSSMETMM ET+ 
Sbjct: 424 RKGTVMAMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLR 483

Query: 484 ECERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGR 543
           +CER  SAGETKRCVGS+EDMIDFATSVLGRN+ VRTTENT+GSKG IMIGAVKGI GG+
Sbjct: 484 DCERTPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGK 543

Query: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHG 603
           VTESVSCHQSLFPYLLYYCHSVPKVRVYEA+LLDPTSKA IN GVAICHLDTSAWSPAHG
Sbjct: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKATINHGVAICHLDTSAWSPAHG 603

Query: 604 AFIALASAPGRVEVCHWIFENDMTWTPAD 628
           AFIAL SAPGRVEVCHWIFENDMTW+ AD
Sbjct: 604 AFIALGSAPGRVEVCHWIFENDMTWSVAD 623

[Close]

BLAST of MS000887 vs. ExPASy TrEMBL
Match: A0A6J1K271 (polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491752 PE=4 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 2.7e-301
Identity = 533/629 (84.74%), Postives = 569/629 (90.46%), Query Frame = 0


Query: 4   LSPPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPT 63
           L  PLL+    LY+ +F L  P  V  A +   EDNPFTPKAFL RYWNKEV ND+PKPT
Sbjct: 4   LPAPLLVL---LYLFIFLLS-PLNVPAAAD---EDNPFTPKAFLNRYWNKEVRNDMPKPT 63

Query: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAV 123
           FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHD  S+FAV
Sbjct: 64  FLFSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDSTSNFAV 123

Query: 124 YANKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQ 183
           Y+NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTDQF++YGTDTNVPDQ
Sbjct: 124 YSNKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDQFSTYGTDTNVPDQ 183

Query: 184 SFNTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFAN 243
           SFNTY +KA+GGNG FT YEQS NVPNLRFT+YSS+ATGRSQSFKSYSENANAGDQSF N
Sbjct: 184 SFNTYASKAVGGNGGFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVN 243

Query: 244 YGKRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEG 303
           YGKRGNGGPNEFTGYGTNANV+GSGFSSYGA GNGANDTFTNYGNEQNNPQNNFRSYG+G
Sbjct: 244 YGKRGNGGPNEFTGYGTNANVIGSGFSSYGANGNGANDTFTNYGNEQNNPQNNFRSYGDG 303

Query: 304 GNGAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQ 363
           GNGAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFANYGKSFNEGTDKFSGYG+NGNGQ
Sbjct: 304 GNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSFNEGTDKFSGYGQNGNGQ 363

Query: 364 SVGFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESML 423
           SVGFKTYGVNTTFKDYAK+GI FAKYSNVS  SGG KM V+     RWVEPGKFFRESML
Sbjct: 364 SVGFKTYGVNTTFKDYAKQGISFAKYSNVS--SGGAKMSVSESLAKRWVEPGKFFRESML 423

Query: 424 RKGAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVT 483
           RKG V+ MPDIRDKMP+RSFLPRSILSKLPFSSSKL  MK IFHAGDNSSMETMM ET+ 
Sbjct: 424 RKGTVVAMPDIRDKMPKRSFLPRSILSKLPFSSSKLEVMKQIFHAGDNSSMETMMTETLR 483

Query: 484 ECERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGR 543
           +CER  SAGETKRCVGS+EDMIDFATSVLGRN+ VRTTENT+GSKG IMIGAVKGI GG+
Sbjct: 484 DCERTPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGAVKGIEGGK 543

Query: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHG 603
           VTESVSCHQSLFPYLLYYCHSVPKVRVYEA+LLDPTSKA IN GVAICHLDTSAWSPAHG
Sbjct: 544 VTESVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPTSKAIINHGVAICHLDTSAWSPAHG 603

Query: 604 AFIALASAPGRVEVCHWIFENDMTWTPAD 628
           AFIAL SAPGRVEVCHWIFENDMTW+ AD
Sbjct: 604 AFIALGSAPGRVEVCHWIFENDMTWSVAD 623

[Close]

BLAST of MS000887 vs. ExPASy TrEMBL
Match: A0A0A0LK44 (BURP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348230 PE=4 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 7.4e-299
Identity = 527/627 (84.05%), Postives = 569/627 (90.75%), Query Frame = 0


Query: 6   PPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL 65
           P L + P  LY+ +F L     VS AG+   ED+PFTPKAFL RYWNKEV ND PKP+FL
Sbjct: 5   PTLYVVP--LYLFIFSLS-SLNVSTAGD---EDDPFTPKAFLNRYWNKEVRNDFPKPSFL 64

Query: 66  FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA 125
           FSK SPLTAVQSAAFAKLAA+NALSSRLP+FCSAANLLCFPDLSPSLAKHD+DS+FAVY+
Sbjct: 65  FSKASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYS 124

Query: 126 NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF 185
           NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTD+F+ YGTDTNVPDQSF
Sbjct: 125 NKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSVYGTDTNVPDQSF 184

Query: 186 NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG 245
           NTY +KAIGG+GKFT YEQS NVPNLRFT+YSS+ATGRSQSFKSYSENANAGDQSF NYG
Sbjct: 185 NTYASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYG 244

Query: 246 KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN 305
           KRGNGGPNEFTGYGT+ANV+GS FSSYGAEGNGANDTFTNYGN+QNNPQNNFRSYGEGGN
Sbjct: 245 KRGNGGPNEFTGYGTSANVIGSRFSSYGAEGNGANDTFTNYGNDQNNPQNNFRSYGEGGN 304

Query: 306 GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV 365
           GAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFA+YGKSFNEGTDKFSGYG+NGNGQSV
Sbjct: 305 GAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSV 364

Query: 366 GFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESMLRK 425
           GFKTYGVNTTFKDYAK+GI FAKYSNVS  SGG KM V+     RWVEPGKFFRESMLRK
Sbjct: 365 GFKTYGVNTTFKDYAKQGINFAKYSNVS--SGGAKMSVSGSLAKRWVEPGKFFRESMLRK 424

Query: 426 GAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTEC 485
           G+VM MPDIRDKMP+RSFLPRSILSKLPFSSSKLG MK IFHAGDNSSMETMM ETV EC
Sbjct: 425 GSVMAMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVREC 484

Query: 486 ERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVT 545
           ER  SAGETKRCVGS+EDMIDFATSVLGRN+ V+TTENT+GSKG IMIG VKG+NGG+VT
Sbjct: 485 ERLPSAGETKRCVGSVEDMIDFATSVLGRNVVVQTTENTKGSKGNIMIGKVKGMNGGKVT 544

Query: 546 ESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAF 605
           ESVSCHQSLFPYLLYYCHSVPKVRVYE DLLDP +KA +N GVAICHLDTSAWSPAHGAF
Sbjct: 545 ESVSCHQSLFPYLLYYCHSVPKVRVYEVDLLDPATKATVNHGVAICHLDTSAWSPAHGAF 604

Query: 606 IALASAPGRVEVCHWIFENDMTWTPAD 628
           IAL SAPGRVEVCHWIFENDMTWT AD
Sbjct: 605 IALGSAPGRVEVCHWIFENDMTWTVAD 623

[Close]

BLAST of MS000887 vs. ExPASy TrEMBL
Match: A0A5A7V5U8 (Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002300 PE=4 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 3.1e-297
Identity = 524/627 (83.57%), Postives = 566/627 (90.27%), Query Frame = 0


Query: 6   PPLLIRPCNLYIILFFLPLPKQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFL 65
           P L + P  LY+ +F L     V   G+   EDNPFTPKAFL RYWNKEV ND+PKP+FL
Sbjct: 5   PTLHVVP--LYLFIFSLS-ALNVPAVGD---EDNPFTPKAFLNRYWNKEVRNDIPKPSFL 64

Query: 66  FSKVSPLTAVQSAAFAKLAAQNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYA 125
           FSK SPLTAVQSAAFAKLAA+NALSSRLP+FCSAANLLCFPDLSPSLAKHD+DS+FAVY+
Sbjct: 65  FSKASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYS 124

Query: 126 NKNFTNYGSQSPGGIDSFKNYSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSF 185
           NKNFT YG+Q  GGIDSFKNYS  +NVVVDSFRRYS E+LGHTD+F++YGT+TNVPDQSF
Sbjct: 125 NKNFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSF 184

Query: 186 NTYGTKAIGGNGKFTNYEQSTNVPNLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYG 245
           NTY +KAIGG+GKFT YEQS NVPNLRFT+YSS+ATGRSQ+FKSYSENANAGDQSF NYG
Sbjct: 185 NTYASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYG 244

Query: 246 KRGNGGPNEFTGYGTNANVVGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGN 305
           KRGNGGPNEFTGYGT+ANVVGS FSSYG +GNGANDTFTNYGN+QNNPQNNFRSYGEGGN
Sbjct: 245 KRGNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGN 304

Query: 306 GAIESFANYRDKANVGDDSFQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSV 365
           GAIESF++YRD+ANVGDDSFQSYSK+SNSA+VNFA+YGKSFNEGTDKFSGYG+NGNGQSV
Sbjct: 305 GAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSV 364

Query: 366 GFKTYGVNTTFKDYAKEGIRFAKYSNVSSGSGGGKMWVN-----RWVEPGKFFRESMLRK 425
           GFK YGVNTTFKDYAK+GI FAKYSNVS  SGG K  V+     RWVEPGKFFRESMLRK
Sbjct: 365 GFKVYGVNTTFKDYAKQGINFAKYSNVS--SGGAKTSVSGSLAKRWVEPGKFFRESMLRK 424

Query: 426 GAVMPMPDIRDKMPRRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTEC 485
           G+VM MPDIRDKMP+RSFLPRSILSKLPFSSSKLG MK IFHAGDNSSMETMM ETV EC
Sbjct: 425 GSVMAMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVREC 484

Query: 486 ERAASAGETKRCVGSMEDMIDFATSVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVT 545
           ER  SAGETKRCVGS+EDMIDFATSVLGRN+ VRTTENT+GSKG IMIG VKG+NGG+VT
Sbjct: 485 ERVPSAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVT 544

Query: 546 ESVSCHQSLFPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAF 605
           ESVSCHQSLFPYLLYYCHSVPKVRVYE DLLDP SKA IN GVAICHLDTSAWSPAHGAF
Sbjct: 545 ESVSCHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAF 604

Query: 606 IALASAPGRVEVCHWIFENDMTWTPAD 628
           IAL SAPGRVEVCHWIFENDMTWT AD
Sbjct: 605 IALGSAPGRVEVCHWIFENDMTWTVAD 623

[Close]

BLAST of MS000887 vs. TAIR 10
Match: AT1G70370.1 (polygalacturonase 2 )

HSP 1 Score: 798.9 bits (2062), Expect = 2.9e-231
Identity = 390/599 (65.11%), Postives = 473/599 (78.96%), Query Frame = 0


Query: 32  GEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAAQNALSS 91
           G    E+NPFTPKA L+RYWNKE+    P+  FL SK SPL AV SA F+KLAA N+L +
Sbjct: 28  GGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPT 87

Query: 92  RLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKNYSQSEN 151
           R P+FCSAANL CFPDL  SL KHD D  F+VY  KNFTNYG+   GG DSFKNYS+  N
Sbjct: 88  RFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGN 147

Query: 152 VVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQSTNVPNL 211
           VV DSFRRYSR + GH D+FT YG ++NV ++ FN+YGT   GG G FTNY+ + N P  
Sbjct: 148 VVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTS 207

Query: 212 RFTSYSSEATGRSQSFKSYSENANAGD-QSFANYGKRGNGGPNEFTGYGTNANVVGSGFS 271
           RFT+YS    GRSQ+FK+Y+  ANAG+ QSF +YGK GNG PNEFT YG ++NV+GSGFS
Sbjct: 208 RFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFS 267

Query: 272 SYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDSFQSYSK 331
           +YG  GN ANDTFT+YG++ N PQNNF +YG  GN A+++FANYRDKANVGDDSF SY+K
Sbjct: 268 NYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAK 327

Query: 332 DSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGIRFAKY- 391
           DSNS +VNF NYG+SFN G++ F+GYGK   G  + FKTY  N+TFKDYAK+G+ FAKY 
Sbjct: 328 DSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKYN 387

Query: 392 -SNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSILSKLP 451
            S  ++ + G    VN+W+EPGKFFRES L++G V+PMPDI+DKMP+RSFLPRSI++KLP
Sbjct: 388 VSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLP 447

Query: 452 FSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFATSVLG 511
           FS+SKLG +K IFHA +NS+M  ++ + VTECER  S GETKRCVGS EDMIDFATSVLG
Sbjct: 448 FSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLG 507

Query: 512 RNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVRVYEA 571
           R++ +RTTEN  GSK K++IG V GINGG++T++VSCHQSL+PYLLYYCHSVPKVRVYEA
Sbjct: 508 RSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEA 567

Query: 572 DLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWTPAD 628
           DLL+  SK KIN G+AICH+DTS+W P+HGAF+AL S PGR+EVCHWIFENDM W  AD
Sbjct: 568 DLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

[Close]

BLAST of MS000887 vs. TAIR 10
Match: AT1G70370.2 (polygalacturonase 2 )

HSP 1 Score: 798.9 bits (2062), Expect = 2.9e-231
Identity = 390/599 (65.11%), Postives = 473/599 (78.96%), Query Frame = 0


Query: 32  GEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAAQNALSS 91
           G    E+NPFTPKA L+RYWNKE+    P+  FL SK SPL AV SA F+KLAA N+L +
Sbjct: 28  GGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKASPLNAVDSATFSKLAAANSLPT 87

Query: 92  RLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKNYSQSEN 151
           R P+FCSAANL CFPDL  SL KHD D  F+VY  KNFTNYG+   GG DSFKNYS+  N
Sbjct: 88  RFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNFTNYGNARAGGADSFKNYSKDGN 147

Query: 152 VVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQSTNVPNL 211
           VV DSFRRYSR + GH D+FT YG ++NV ++ FN+YGT   GG G FTNY+ + N P  
Sbjct: 148 VVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYGTFGTGGAGDFTNYQNNVNNPTS 207

Query: 212 RFTSYSSEATGRSQSFKSYSENANAGD-QSFANYGKRGNGGPNEFTGYGTNANVVGSGFS 271
           RFT+YS    GRSQ+FK+Y+  ANAG+ QSF +YGK GNG PNEFT YG ++NV+GSGFS
Sbjct: 208 RFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNGNGVPNEFTSYGVSSNVIGSGFS 267

Query: 272 SYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDSFQSYSK 331
           +YG  GN ANDTFT+YG++ N PQNNF +YG  GN A+++FANYRDKANVGDDSF SY+K
Sbjct: 268 NYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAVDTFANYRDKANVGDDSFSSYAK 327

Query: 332 DSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGIRFAKY- 391
           DSNS +VNF NYG+SFN G++ F+GYGK   G  + FKTY  N+TFKDYAK+G+ FAKY 
Sbjct: 328 DSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFKTYTPNSTFKDYAKKGVAFAKYN 387

Query: 392 -SNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSILSKLP 451
            S  ++ + G    VN+W+EPGKFFRES L++G V+PMPDI+DKMP+RSFLPRSI++KLP
Sbjct: 388 VSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIPMPDIKDKMPKRSFLPRSIITKLP 447

Query: 452 FSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFATSVLG 511
           FS+SKLG +K IFHA +NS+M  ++ + VTECER  S GETKRCVGS EDMIDFATSVLG
Sbjct: 448 FSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPSVGETKRCVGSAEDMIDFATSVLG 507

Query: 512 RNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVRVYEA 571
           R++ +RTTEN  GSK K++IG V GINGG++T++VSCHQSL+PYLLYYCHSVPKVRVYEA
Sbjct: 508 RSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSCHQSLYPYLLYYCHSVPKVRVYEA 567

Query: 572 DLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWTPAD 628
           DLL+  SK KIN G+AICH+DTS+W P+HGAF+AL S PGR+EVCHWIFENDM W  AD
Sbjct: 568 DLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGSKPGRIEVCHWIFENDMNWAIAD 626

[Close]

BLAST of MS000887 vs. TAIR 10
Match: AT1G23760.1 (BURP domain-containing protein )

HSP 1 Score: 783.5 bits (2022), Expect = 1.3e-226
Identity = 384/603 (63.68%), Postives = 468/603 (77.61%), Query Frame = 0


Query: 26  KQVSGAGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAA 85
           ++++  G +P E NPFTPKA L+RYWNK++ +  P   FL SK SPLTAV SA FAKLA+
Sbjct: 24  ERLTNGGFSP-EINPFTPKASLVRYWNKQIHHQSPGSAFLLSKASPLTAVASATFAKLAS 83

Query: 86  QNALSSRLPEFCSAANLLCFPDLSPSLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKN 145
           +NAL  RLP+FCSAANL CFPDL P+L KH  D  F+VY  +NFTNYG+  PGG DSFK 
Sbjct: 84  ENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRNFTNYGTIHPGGADSFKK 143

Query: 146 YSQSENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQS 205
           YSQ+ N V DSFRRYSR++ GH D+FT Y  ++NV ++ FN+YGT A GG+G F NY+  
Sbjct: 144 YSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSYGTSATGGSGDFKNYQSG 203

Query: 206 TNVPNLRFTSYSSEATGRSQSFKSYSENANAG-DQSFANYGKRGNGGPNEFTGYGTNANV 265
            N P  RF +YS E  GR+Q+FK+Y+  AN+G  QSF +YGK GNG PNEF+ YG ++NV
Sbjct: 204 VNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKNGNGAPNEFSSYGVSSNV 263

Query: 266 VGSGFSSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDS 325
           +GS FS+YG  GN ANDTF  YG + N PQNNF+SYG  GN A+++F NYRD ANVGDDS
Sbjct: 264 IGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAAVDTFLNYRDNANVGDDS 323

Query: 326 FQSYSKDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGI 385
           F SY+K+SN  +V+F NYGKS N G++ FSGYGK   G  + FKTY  N+TFKDY K G+
Sbjct: 324 FSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKIDFKTYTQNSTFKDYTKTGV 383

Query: 386 RFAKYSNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSIL 445
            FAKY+   S  GGGK  VN+WVEPGKFFRESML++G ++ MPDI+DKMP+RSFLPRSI+
Sbjct: 384 EFAKYNR--SSLGGGKT-VNKWVEPGKFFRESMLKEGTLIWMPDIKDKMPKRSFLPRSIV 443

Query: 446 SKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFAT 505
           SKLPFS+SK+  +K +FHA DNS+ME ++ + V ECER  +  ETKRCVGS EDMIDFAT
Sbjct: 444 SKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPPTVSETKRCVGSAEDMIDFAT 503

Query: 506 SVLGRNLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVR 565
           SVLGR++ +RTTE+  GSK K+MIG V GINGGRVT+SVSCHQSL+PYLLYYCHSVPKVR
Sbjct: 504 SVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVSCHQSLYPYLLYYCHSVPKVR 563

Query: 566 VYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWT 625
           VYE+DLLDP SKAKIN G+AICH+DTSAW   HGAF+ L S PG++EVCHWIFENDM W 
Sbjct: 564 VYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLGSRPGQIEVCHWIFENDMNWA 622

Query: 626 PAD 628
            AD
Sbjct: 624 IAD 622

[Close]

BLAST of MS000887 vs. TAIR 10
Match: AT1G60390.1 (polygalacturonase 1 )

HSP 1 Score: 758.1 bits (1956), Expect = 5.8e-219
Identity = 376/598 (62.88%), Postives = 453/598 (75.75%), Query Frame = 0


Query: 31  AGEAPTEDNPFTPKAFLIRYWNKEVSNDLPKPTFLFSKVSPLTAVQSAAFAKLAAQNALS 90
           AG     +NPFTPKA LIRYWN  ++ D PKP+F  SK SPLTAV S  FA LA+ +AL+
Sbjct: 30  AGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSKASPLTAVDSTRFASLASNHALN 89

Query: 91  SRLPEFCSAANLLCFPDLSP-SLAKHDKDSSFAVYANKNFTNYGSQSPGGIDSFKNYSQS 150
           +   +FCSAA L CFP+L+  SL KH  D +FA Y+ KNFTNYGS    G DSFKNYS  
Sbjct: 90  THHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGKNFTNYGSDRLSGADSFKNYSGG 149

Query: 151 ENVVVDSFRRYSRESLGHTDQFTSYGTDTNVPDQSFNTYGTKAIGGNGKFTNYEQSTNVP 210
           +N+ VDSFRRYSR S GH D FT+Y  + NV DQSF TY T   GG+G+FTNY    N P
Sbjct: 150 DNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTTYATGTTGGSGEFTNYNTDANEP 209

Query: 211 NLRFTSYSSEATGRSQSFKSYSENANAGDQSFANYGKRGNGGPNEFTGYGTNANVVGSGF 270
           N RFTSYS +A GRSQ+F +YSEN N G QSF +Y K GNG PNEF+GYGT +NVV +GF
Sbjct: 210 NGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKNGNGAPNEFSGYGTGSNVVNTGF 269

Query: 271 SSYGAEGNGANDTFTNYGNEQNNPQNNFRSYGEGGNGAIESFANYRDKANVGDDSFQSYS 330
           + YG   NGAND+FT+YG   N P N F+ YG+GGNGA+  F NYRD++N+G DSF SY+
Sbjct: 270 TKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGAVYGFKNYRDQSNIGVDSFSSYA 329

Query: 331 KDSNSAEVNFANYGKSFNEGTDKFSGYGKNGNGQSVGFKTYGVNTTFKDYAKEGIRFAKY 390
           K+SN+ +VNF NYGKSFN G+D F+GYG++  G +V FKTYG   +FK Y K+G+ FA+Y
Sbjct: 330 KNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSFKTYGQGQSFKVYTKDGVVFARY 389

Query: 391 SNVSSGSGGGKMWVNRWVEPGKFFRESMLRKGAVMPMPDIRDKMPRRSFLPRSILSKLPF 450
           SN  S +G     VN+WVE GKFFRE+ML++G +M MPDI+DKMP+R+FLPR+I+  LPF
Sbjct: 390 SNNVSSNG---KTVNKWVEEGKFFREAMLKEGTLMQMPDIKDKMPKRTFLPRNIVKNLPF 449

Query: 451 SSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVGSMEDMIDFATSVLGR 510
           SSS +G +  +F AG+NSSM  ++   V+ECER AS GETKRCVGS EDMIDFATSVLGR
Sbjct: 450 SSSTIGEIWRVFGAGENSSMAGIISSAVSECERPASHGETKRCVGSAEDMIDFATSVLGR 509

Query: 511 NLAVRTTENTEGSKGKIMIGAVKGINGGRVTESVSCHQSLFPYLLYYCHSVPKVRVYEAD 570
            + VRTTEN  GSK K++IG V GINGG VT +VSCHQSL+PYLLYYCHSVP+VRVYE D
Sbjct: 510 GVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVSCHQSLYPYLLYYCHSVPRVRVYETD 569

Query: 571 LLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGRVEVCHWIFENDMTWTPAD 628
           LLDP S  KIN GVAICH+DTSAWSP+HGAF+AL S PG++EVCHWIFENDMTW   D
Sbjct: 570 LLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALGSGPGQIEVCHWIFENDMTWNIID 624

[Close]

BLAST of MS000887 vs. TAIR 10
Match: AT5G25610.1 (BURP domain-containing protein )

HSP 1 Score: 118.2 bits (295), Expect = 2.3e-26
Identity = 68/194 (35.05%), Postives = 97/194 (50.00%), Query Frame = 0


Query: 435 RRSFLPRSILSKLPFSSSKLGAMKHIFHAGDNSSMETMMRETVTECERAASAGETKRCVG 494
           + +FLPR     +PF S K       F     S    MM++T+ ECE    +GE K C  
Sbjct: 201 KTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEEAEMMKKTIEECEARKVSGEEKYCAT 260

Query: 495 SMEDMIDFATSVLGR-NLAVRTTE----NTEGSKGKIMIGAVKGINGGRVTESVSCHQSL 554
           S+E M+DF+ S LG+ ++   +TE    N    K KI    VK ++  +   SV CH+  
Sbjct: 261 SLESMVDFSVSKLGKYHVRAVSTEVAKKNAPMQKYKIAAAGVKKLSDDK---SVVCHKQK 320

Query: 555 FPYLLYYCHSVPKVRVYEADLLDPTSKAKINRGVAICHLDTSAWSPAHGAFIALASAPGR 614
           +P+ ++YCH      VY   L          + VA+CH +TSAW+P H AF  L   PG 
Sbjct: 321 YPFAVFYCHKAMMTTVYAVPLEGENGMRA--KAVAVCHKNTSAWNPNHLAFKVLKVKPGT 380

Query: 615 VEVCHWIFENDMTW 624
           V VCH++ E  + W
Sbjct: 381 VPVCHFLPETHVVW 389

The following BLAST results are available for this feature:

BLAST of MS000887 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159130.1	0.0e+00	97.43	polygalacturonase 1 beta-like protein 3 [Momordica charantia]	[more]
XP_023546090.1	5.1e-302	85.21	polygalacturonase 1 beta-like protein 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022961767.1	6.7e-302	85.06	polygalacturonase 1 beta-like protein 3 [Cucurbita moschata]	[more]
KAG6598207.1	1.9e-301	84.90	Polygalacturonase 1 beta-like protein 3, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7029192.1	3.3e-301	84.90	Polygalacturonase 1 beta-like protein 3 [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of MS000887 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92990	4.1e-230	65.11	Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=PGL3 ...	[more]
P92982	1.8e-225	63.68	Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PGL1 ...	[more]
Q40161	6.4e-223	62.46	Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum OX=4081 G...	[more]
O80760	8.1e-218	62.88	Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PGL2 ...	[more]
P93217	1.8e-217	61.09	Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum OX=4081 G...	[more]

BLAST of MS000887 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DXS8	0.0e+00	97.43	polygalacturonase 1 beta-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1HB03	3.2e-302	85.06	polygalacturonase 1 beta-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1K271	2.7e-301	84.74	polygalacturonase 1 beta-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111491...	[more]
A0A0A0LK44	7.4e-299	84.05	BURP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G348230 PE=4 S...	[more]
A0A5A7V5U8	3.1e-297	83.57	Polygalacturonase 1 beta-like protein 3 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]

BLAST of MS000887 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70370.1	2.9e-231	65.11	polygalacturonase 2	[more]
AT1G70370.2	2.9e-231	65.11	polygalacturonase 2	[more]
AT1G23760.1	1.3e-226	63.68	BURP domain-containing protein	[more]
AT1G60390.1	5.8e-219	62.88	polygalacturonase 1	[more]
AT5G25610.1	2.3e-26	35.05	BURP domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004873	BURP domain	SMART	SM01045	BURP_2	coord: 410..626 e-value: 1.3E-126 score: 436.6
IPR004873	BURP domain	PFAM	PF03181	BURP	coord: 412..624 e-value: 2.8E-64 score: 216.6
IPR004873	BURP domain	PROSITE	PS51277	BURP	coord: 412..626 score: 72.322517
None	No IPR available	PANTHER	PTHR31458:SF2	POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2	coord: 17..627
None	No IPR available	PANTHER	PTHR31458	POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2	coord: 17..627

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000887.1	MS000887.1	mRNA