MS000534 (gene) Bitter gourd (TR) v1

Overview


Name	MS000534
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Glucan endo-1,3-beta-glucosidase 3
Location	scaffold64: 123493 .. 124761 (-)
RNA-Seq Expression	MS000534
Synteny	MS000534

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGGGGTTAATCTCGGGACCGATGTCTCCAAGCTTCCATCAGCTTCAGATACAGTTGCAATTCTTAAATCCCATCAAATTACTCATCTCCGCCTTTATAATGCTGATGCTCAATTGCTAAAGGCCCTAGCAAACAGTAGCATCGAAGTAATTGTTGGCGTCACAAATGAGGAAGTTCTCAGGATTGGTGAATCTCCAGCAGTGGCTGCAGCTTGGGTTAACAAAAATGTTGCTGCTTACTTGCCAGCAACGAATATTACAGCCATTGCTGTTGGCAGTGAGGTCCTTACAGCAATTCCTCATGTTGGCCCAGTCTTAGTTCCGGCCATGTATTCCATACATAAAGCCTTGGTTGCTGCCAACCTAAACTCTATGATCAAAGTTTCCACTCCCCAATCAATGGACATAATACCCCGAGCTTTTCCCCCGTCCACCGCTACCTTTAATGCTTCGTGGAACTCTACAATCTATCAGCTCCTTCAGTTTTTAAATAACACGAAGTCCTACTACATGTTGAATGCCTATCCTTATTACGGTTACACTAGTGGGAATGGAATTTTTCCGCTTGATTATGCACTTTTTCGATCACTTCCTGCAATTAAGCAGATTGTTGATCCGAACACTCTTTTCCACTATAATAGTATGTTTGATGCTATGGTTGATGCAACCTATTACTCAATCGATGCTTTCAATTTTTCCGGGATTACCGTTGTGGTTACTGAGACTGGTTGGCCATGGTTTGGTGGAGCAAATGAACCTGATGCAACAATTCAAAATGCTGGCACCTACTGTAACAATCTGATCAGGAGAGTCACCAACGATTCAGGTCCACCCAGTCAGCCAACTATACCAATCAACACGTATCTGTATGAGTTGTTTAACGAAGATAAGAAGCCTGGCCCGGTCTCGGAAAAGAACTGGGGTATACTTTTCCCCAATGGCTCTGCTGTTTATCCTTTGGGTTTGACTTCCGGCCGTGCCACAGCAAATTCTTCTGCAGTTTATTGTGTGGCAAAAGACGGTGCCGATGAAGATAAATTGCAAGATGGCCTGAATTGGGCGTGTGGACAAGGAGGAGCAAACTGTGTTGCCATTCAGCCGGGGAGGCCATGCTATCTTCCGAATAACCTTACTAACCATGCTTCTTATGCATTTAATGATTATTATCAGAAAATGCATAGTGCTGGTGGAACATGCGACTTTGACGGTACCGCTATGATTACGACGATCGATCCCAGTAAGTTCTTCTTACTGTTTTTCTCCTTGTTC

mRNA sequence

GTGGGGGTTAATCTCGGGACCGATGTCTCCAAGCTTCCATCAGCTTCAGATACAGTTGCAATTCTTAAATCCCATCAAATTACTCATCTCCGCCTTTATAATGCTGATGCTCAATTGCTAAAGGCCCTAGCAAACAGTAGCATCGAAGTAATTGTTGGCGTCACAAATGAGGAAGTTCTCAGGATTGGTGAATCTCCAGCAGTGGCTGCAGCTTGGGTTAACAAAAATGTTGCTGCTTACTTGCCAGCAACGAATATTACAGCCATTGCTGTTGGCAGTGAGGTCCTTACAGCAATTCCTCATGTTGGCCCAGTCTTAGTTCCGGCCATGTATTCCATACATAAAGCCTTGGTTGCTGCCAACCTAAACTCTATGATCAAAGTTTCCACTCCCCAATCAATGGACATAATACCCCGAGCTTTTCCCCCGTCCACCGCTACCTTTAATGCTTCGTGGAACTCTACAATCTATCAGCTCCTTCAGTTTTTAAATAACACGAAGTCCTACTACATGTTGAATGCCTATCCTTATTACGGTTACACTAGTGGGAATGGAATTTTTCCGCTTGATTATGCACTTTTTCGATCACTTCCTGCAATTAAGCAGATTGTTGATCCGAACACTCTTTTCCACTATAATAGTATGTTTGATGCTATGGTTGATGCAACCTATTACTCAATCGATGCTTTCAATTTTTCCGGGATTACCGTTGTGGTTACTGAGACTGGTTGGCCATGGTTTGGTGGAGCAAATGAACCTGATGCAACAATTCAAAATGCTGGCACCTACTGTAACAATCTGATCAGGAGAGTCACCAACGATTCAGGTCCACCCAGTCAGCCAACTATACCAATCAACACGTATCTGTATGAGTTGTTTAACGAAGATAAGAAGCCTGGCCCGGTCTCGGAAAAGAACTGGGGTATACTTTTCCCCAATGGCTCTGCTGTTTATCCTTTGGGTTTGACTTCCGGCCGTGCCACAGCAAATTCTTCTGCAGTTTATTGTGTGGCAAAAGACGGTGCCGATGAAGATAAATTGCAAGATGGCCTGAATTGGGCGTGTGGACAAGGAGGAGCAAACTGTGTTGCCATTCAGCCGGGGAGGCCATGCTATCTTCCGAATAACCTTACTAACCATGCTTCTTATGCATTTAATGATTATTATCAGAAAATGCATAGTGCTGGTGGAACATGCGACTTTGACGGTACCGCTATGATTACGACGATCGATCCCAGTAAGTTCTTCTTACTGTTTTTCTCCTTGTTC

Coding sequence (CDS)

GTGGGGGTTAATCTCGGGACCGATGTCTCCAAGCTTCCATCAGCTTCAGATACAGTTGCAATTCTTAAATCCCATCAAATTACTCATCTCCGCCTTTATAATGCTGATGCTCAATTGCTAAAGGCCCTAGCAAACAGTAGCATCGAAGTAATTGTTGGCGTCACAAATGAGGAAGTTCTCAGGATTGGTGAATCTCCAGCAGTGGCTGCAGCTTGGGTTAACAAAAATGTTGCTGCTTACTTGCCAGCAACGAATATTACAGCCATTGCTGTTGGCAGTGAGGTCCTTACAGCAATTCCTCATGTTGGCCCAGTCTTAGTTCCGGCCATGTATTCCATACATAAAGCCTTGGTTGCTGCCAACCTAAACTCTATGATCAAAGTTTCCACTCCCCAATCAATGGACATAATACCCCGAGCTTTTCCCCCGTCCACCGCTACCTTTAATGCTTCGTGGAACTCTACAATCTATCAGCTCCTTCAGTTTTTAAATAACACGAAGTCCTACTACATGTTGAATGCCTATCCTTATTACGGTTACACTAGTGGGAATGGAATTTTTCCGCTTGATTATGCACTTTTTCGATCACTTCCTGCAATTAAGCAGATTGTTGATCCGAACACTCTTTTCCACTATAATAGTATGTTTGATGCTATGGTTGATGCAACCTATTACTCAATCGATGCTTTCAATTTTTCCGGGATTACCGTTGTGGTTACTGAGACTGGTTGGCCATGGTTTGGTGGAGCAAATGAACCTGATGCAACAATTCAAAATGCTGGCACCTACTGTAACAATCTGATCAGGAGAGTCACCAACGATTCAGGTCCACCCAGTCAGCCAACTATACCAATCAACACGTATCTGTATGAGTTGTTTAACGAAGATAAGAAGCCTGGCCCGGTCTCGGAAAAGAACTGGGGTATACTTTTCCCCAATGGCTCTGCTGTTTATCCTTTGGGTTTGACTTCCGGCCGTGCCACAGCAAATTCTTCTGCAGTTTATTGTGTGGCAAAAGACGGTGCCGATGAAGATAAATTGCAAGATGGCCTGAATTGGGCGTGTGGACAAGGAGGAGCAAACTGTGTTGCCATTCAGCCGGGGAGGCCATGCTATCTTCCGAATAACCTTACTAACCATGCTTCTTATGCATTTAATGATTATTATCAGAAAATGCATAGTGCTGGTGGAACATGCGACTTTGACGGTACCGCTATGATTACGACGATCGATCCCAGTAAGTTCTTCTTACTGTTTTTCTCCTTGTTC

Protein sequence

VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVLRIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAANLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVTETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPGPVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFFLLFFSLF

Homology

[Close]

BLAST of MS000534 vs. NCBI nr
Match: XP_022153235.1 (glucan endo-1,3-beta-glucosidase 4-like [Momordica charantia] >XP_022153236.1 glucan endo-1,3-beta-glucosidase 4-like [Momordica charantia] >XP_022153237.1 glucan endo-1,3-beta-glucosidase 4-like [Momordica charantia] >XP_022153238.1 glucan endo-1,3-beta-glucosidase 4-like [Momordica charantia] >XP_022153240.1 glucan endo-1,3-beta-glucosidase 4-like [Momordica charantia])

HSP 1 Score: 830.9 bits (2145), Expect = 5.1e-237
Identity = 410/413 (99.27%), Postives = 411/413 (99.52%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA
Sbjct: 85  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY
Sbjct: 145 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGI VVVT
Sbjct: 205 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPWFGG NEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG
Sbjct: 265 ETGWPWFGGGNEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           PVSEKNWGILFPNGSAVYPLG+TSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA
Sbjct: 325 PVSEKNWGILFPNGSAVYPLGVTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
           NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS
Sbjct: 385 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 437

[Close]

BLAST of MS000534 vs. NCBI nr
Match: XP_038879038.1 (glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida] >XP_038879039.1 glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida] >XP_038879040.1 glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida])

HSP 1 Score: 774.6 bits (1999), Expect = 4.3e-220
Identity = 381/419 (90.93%), Postives = 395/419 (94.27%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVS LPSASD VAILKSHQITHLRLYNAD+Q+LKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADSQMLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAAYLP TNITAIAVGSEVLT IPHVGPVLVPAMYS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTTIPHVGPVLVPAMYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN +IKVSTPQSMDIIPR FPPSTA+F ASWNSTIYQLLQFL NTKSYYMLNAYPYYGY
Sbjct: 145 NLNYLIKVSTPQSMDIIPRPFPPSTASFEASWNSTIYQLLQFLKNTKSYYMLNAYPYYGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
            SGNG FPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGI VVVT
Sbjct: 205 NSGNGSFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWP FGGANEPDATIQNAGTY +NLIRRV+NDSGPPSQPTIPINTYLYELFNEDKKPG
Sbjct: 265 ETGWPSFGGANEPDATIQNAGTYISNLIRRVSNDSGPPSQPTIPINTYLYELFNEDKKPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           P+SEKNWGILFPNGSAVYPLGL SGRATANSS VYCVAKDGADEDKLQDGLNWACGQGGA
Sbjct: 325 PISEKNWGILFPNGSAVYPLGLMSGRATANSSTVYCVAKDGADEDKLQDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFFLLF 420
           NC AIQ GRPC+LPNN+TNHASYA+NDYYQKM  AGGTCDFDGTAM+TT+DPS    +F
Sbjct: 385 NCAAIQQGRPCFLPNNITNHASYAYNDYYQKMRGAGGTCDFDGTAMLTTVDPSHGSCIF 443

[Close]

BLAST of MS000534 vs. NCBI nr
Match: KAG7023810.1 (Glucan endo-1,3-beta-glucosidase 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 773.5 bits (1996), Expect = 9.6e-220
Identity = 376/416 (90.38%), Postives = 395/416 (94.95%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVS LPSAS  VAILK+H+ITHLRLYNAD+QLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSNLPSASGIVAILKTHRITHLRLYNADSQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAAYLP TNITAIAVGSEVLTAIPHVGPVLVPAMYS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTAIPHVGPVLVPAMYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN +IKVSTPQSMD+IPR FPPSTATFNASWNSTI QLLQFL NTKSYYMLNAYPYYGY
Sbjct: 145 NLNDLIKVSTPQSMDLIPRPFPPSTATFNASWNSTISQLLQFLKNTKSYYMLNAYPYYGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           T+ NGIFPLDYALFRSLPA+KQIVDPNTLFHYNSMFDAMVDATYYSID F FSGI +VVT
Sbjct: 205 TTENGIFPLDYALFRSLPAVKQIVDPNTLFHYNSMFDAMVDATYYSIDTFKFSGIPIVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPWFGGANEPDATIQNAGTY +NLIRRV+NDSGPPSQPT PINTYLYELFNEDK+PG
Sbjct: 265 ETGWPWFGGANEPDATIQNAGTYISNLIRRVSNDSGPPSQPTTPINTYLYELFNEDKRPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           P+SEKNWGILFPNGSAVYPLGL SGR+T NSSAVYCVAKDGADEDKL+DGLNWACGQGGA
Sbjct: 325 PISEKNWGILFPNGSAVYPLGLISGRSTGNSSAVYCVAKDGADEDKLEDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFF 417
           NC AIQ GRPC+LPNN+TNHASYA+NDYYQK  SAGGTCDFDGTAM+TTIDPSKFF
Sbjct: 385 NCAAIQRGRPCFLPNNITNHASYAYNDYYQKSRSAGGTCDFDGTAMLTTIDPSKFF 440

[Close]

BLAST of MS000534 vs. NCBI nr
Match: KAG7022153.1 (Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 770.8 bits (1989), Expect = 6.2e-219
Identity = 376/413 (91.04%), Postives = 393/413 (95.16%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVS LPSASD VAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAAYLP TNITAIAVGSEVL+ IP VGPVLVPA+YS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLSTIPQVGPVLVPALYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN ++KVSTPQSMD+IPR FPPSTATFNASWN+T+YQLLQFL NTKSYYMLNAYPY GY
Sbjct: 145 NLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNATMYQLLQFLKNTKSYYMLNAYPYLGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGI VVVT
Sbjct: 205 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPW GGANEPDATIQNAGTY NNLIRRV+NDSGPPSQPTIPINTY+YELFNEDK+PG
Sbjct: 265 ETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSGPPSQPTIPINTYIYELFNEDKRPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           PVSEKNWGILF NGS VYPLGL SGRATANSS VYCVAKDGADEDKLQDGLNWACGQGGA
Sbjct: 325 PVSEKNWGILFSNGSTVYPLGLASGRATANSSTVYCVAKDGADEDKLQDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
           NC AIQ GRPC+LPNN+T+HASYA+NDYYQKMH+AGGTCDFDGTA +TTIDPS
Sbjct: 385 NCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHTAGGTCDFDGTATLTTIDPS 437

[Close]

BLAST of MS000534 vs. NCBI nr
Match: XP_022927211.1 (glucan endo-1,3-beta-glucosidase 4-like [Cucurbita moschata] >XP_022927220.1 glucan endo-1,3-beta-glucosidase 4-like [Cucurbita moschata])

HSP 1 Score: 769.2 bits (1985), Expect = 1.8e-218
Identity = 375/413 (90.80%), Postives = 392/413 (94.92%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVS LPSASD VAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAAYLP TNITAIAVGSEVL+ IP  GPVLVPA+YS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLSTIPQAGPVLVPALYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN ++KVSTPQSMD+IPR FPPSTATFNASWN+T+YQLLQFL NTKSYYMLNAYPY GY
Sbjct: 145 NLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNATMYQLLQFLKNTKSYYMLNAYPYLGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGI VVVT
Sbjct: 205 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPW GGANEPDATIQNAGTY NNLIRRV+NDSGPPSQPTIPINTY+YELFNEDK+PG
Sbjct: 265 ETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSGPPSQPTIPINTYIYELFNEDKRPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           PVSEKNWGILF NGS VYPLGL SGRATANSS VYCVAKDGADEDKLQDGLNWACGQGGA
Sbjct: 325 PVSEKNWGILFSNGSTVYPLGLASGRATANSSTVYCVAKDGADEDKLQDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
           NC AIQ GRPC+LPNN+T+HASYA+NDYYQKMH+AGGTCDFDGTA +TTIDPS
Sbjct: 385 NCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHTAGGTCDFDGTATLTTIDPS 437

[Close]

BLAST of MS000534 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 8.0e-177
Identity = 295/415 (71.08%), Postives = 357/415 (86.02%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GTD++ +P  SD V +LKS QITH+RLY+A++ +LKA AN+SIEV+VGVTNEE+L
Sbjct: 26  IGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTSIEVMVGVTNEEIL 85

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           +IG  P+ AAAWVNKNVAAY+P+TNITAIAVGSEVLT IPHV P+L  A+ +IHKALVA+
Sbjct: 86  KIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILASALNNIHKALVAS 145

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN  +KVS+P SMDI+P+ FPPST+TF+ SWN+T+YQLLQFL NT S++MLNAYPYYGY
Sbjct: 146 NLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTGSFFMLNAYPYYGY 205

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           T+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA YYS++A NFS I VVVT
Sbjct: 206 TTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVVT 265

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWP  GG++E  AT+ NA T+  NLI+RV N+SGPPSQP IPINTY+YEL+NEDK+ G
Sbjct: 266 ETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINTYIYELYNEDKRSG 325

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATA--NSSAVYCVAKDGADEDKLQDGLNWACGQG 360
           PVSE+NWGILFPNG++VYPL L+ G ++A  N S+++CVAK  AD+DKL DGLNWACGQG
Sbjct: 326 PVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKADADDDKLVDGLNWACGQG 385

Query: 361 GANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
            ANC AIQPG+PCYLPN++ +HAS+AFNDYYQKM SAGGTCDFDGTA+ TT DPS
Sbjct: 386 RANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of MS000534 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 437.2 bits (1123), Expect = 2.1e-121
Identity = 217/422 (51.42%), Postives = 292/422 (69.19%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GT+V+ +PS +  VA+LKS  I  +RLY+AD  +L A A++ ++VI+ V N+++L
Sbjct: 23  IGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFAHTGVQVIISVPNDQLL 82

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
            I +S A AA WV +NVAAY PATNIT IAVGSEVLT++ +   VLV A+  I  ALV A
Sbjct: 83  GISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAASVLVSALKYIQAALVTA 142

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NL+  IKVSTP S  II  +FPPS A FN +W+  I  LL+FL +T S  +LN YPY+ Y
Sbjct: 143 NLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFLQSTGSPLLLNVYPYFDY 202

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
              NG+ PLDYALF+ L A K+ VD NTL HY ++FDA+VDA Y+++   NF+ I +VVT
Sbjct: 203 VQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAYFAMSYLNFTNIPIVVT 262

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           E+GWP  GG +E DAT++NA TY +NLI+ V N +G P  P   + TY+YEL+NED +PG
Sbjct: 263 ESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTAVTTYIYELYNEDTRPG 322

Query: 301 PVSEKNWGILFPNGSAVYPLGLT-SGRATAN--SSAVYCVAKDGADEDKLQDGLNWACGQ 360
           PVSEKNWG+ + NG+ VY L L  +G   AN  ++  +C+AK+  D   LQ  L+WACG 
Sbjct: 323 PVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKEKVDRKMLQAALDWACGP 382

Query: 361 GGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFFL 420
           G  +C A+  G  CY P+++  H++YAFN YYQKM  A G+CDF G A +TT DPS+   
Sbjct: 383 GKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTC 442

[Close]

BLAST of MS000534 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 430.6 bits (1106), Expect = 2.0e-119
Identity = 218/416 (52.40%), Postives = 291/416 (69.95%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GTD+S +P  +  VA+LK+ +I H+RLYNAD  LL ALAN+ I+VI+ + N+++L
Sbjct: 32  IGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQLL 91

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
            IG+S + AA WV +NV A+ PAT ITA++VGSEVLT++ +  PVLV A+ ++H AL++A
Sbjct: 92  GIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALLSA 151

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NL+ +IKVSTP S  +I   FPPS A FN S N+ I  LL FL +T SY M+N YPY  Y
Sbjct: 152 NLDKLIKVSTPLSTSLILDPFPPSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYIDY 211

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
              NG+ PLDYALF+ +P  K+ VD NTL  Y++ FDAMVDATY+++   NF+ I V+VT
Sbjct: 212 MQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVT 271

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           E+GWP  G  NEPDAT+ NA TY +NLIR V N +G P +P I ++TY+YEL+NED K G
Sbjct: 272 ESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTKAG 331

Query: 301 PVSEKNWGILFPNGSAVYPLGLT-SGRATAN--SSAVYCVAKDGADEDKLQDGLNWACGQ 360
            +SEKNWG+   NG  VY L LT SG   AN  ++  YC A++GAD   LQ  L+WACG 
Sbjct: 332 -LSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTAREGADTKMLQAALDWACGP 391

Query: 361 GGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
           G  +C  I+ G  CY P+N+  HA+YAF+ YY +  +    C+F+G A ITT DPS
Sbjct: 392 GKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

[Close]

BLAST of MS000534 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 422.5 bits (1085), Expect = 5.4e-117
Identity = 215/422 (50.95%), Postives = 284/422 (67.30%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VG N+GTDVS L S ++ V  L++ ++ H+RLY+AD +LLKALA + + VI+ V N ++L
Sbjct: 44  VGFNIGTDVSNLLSPTELVKFLQAQKVNHVRLYDADPELLKALAKTKVRVIISVPNNQLL 103

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
            IG S + AA+W+ +NV AY P T ITAI+VG EVLT +P   P+L+PA+ S++ ALVA+
Sbjct: 104 AIGSSNSTAASWIGRNVVAYYPETLITAISVGDEVLTTVPSSAPLLLPAIESLYNALVAS 163

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NL++ IKVSTP +  I+   FPPS A FN +W+S +  LLQFL+ T S  M+N YPYY Y
Sbjct: 164 NLHTQIKVSTPHAASIMLDTFPPSQAYFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVY 223

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
               G+ PLD  LF  L   K++VDPNTL HY ++ DAMVDA Y S+   N S + V+VT
Sbjct: 224 MQNKGVVPLDNCLFEPLTPSKEMVDPNTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVT 283

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           E+GWP  G + EP ATI NA TY +NLI+ V + +G P  P +  + Y+YELFNED +  
Sbjct: 284 ESGWPSKGDSKEPYATIDNADTYNSNLIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAP 343

Query: 301 PVSEKNWGILFPNGSAVYPLGLT-SGRATAN--SSAVYCVAKDGADEDKLQDGLNWACGQ 360
           PVSE +WG+ + N + VY L ++ SG   AN  ++  YC+A DG D   LQ  L+WACG 
Sbjct: 344 PVSEASWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACGP 403

Query: 361 GGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFFL 420
           G +NC  IQPG  CY PNN+  HAS+AFN YYQK   A G+CDF G AMITT DPS    
Sbjct: 404 GRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPSHGSC 463

[Close]

BLAST of MS000534 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.9e-86
Identity = 171/426 (40.14%), Postives = 250/426 (58.69%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGV  G     LP+ S  V +++ H I ++R+Y+ ++Q+LKA  N+SIE+++GV N ++ 
Sbjct: 26  VGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSDLN 85

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHV--GPVLVPAMYSIHKALV 120
              +S +    W+  +V  Y P T IT I VG+E  T  PH+     +VPAM ++  AL 
Sbjct: 86  AFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAE-STDDPHINASSFVVPAMQNVLTALR 145

Query: 121 AANLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYY 180
              L+  IKVST  S+ I+ R+FPPS   FN+S+   +  +L+FL   KS +M++ YPYY
Sbjct: 146 KVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDLYPYY 205

Query: 181 GYTSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVV 240
            Y        LDY LF S     +++DPNT   Y +MFDA VDA YY++ A NF  I ++
Sbjct: 206 AYRDSPNNVSLDYVLFES---SSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIM 265

Query: 241 VTETGWPWFGGANE-PDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDK 300
           VTETGWP  G   E   A+  NA TY +N+IR V  + G P++P   +N Y++ LFNE++
Sbjct: 266 VTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENR 325

Query: 301 KPGPVSEKNWGILFPNGSAVYPLGLT----------SGRATANSSAVYCVAKDGADEDKL 360
           K G  SE+NWG+ +P+ ++VY L  T          SG  ++ SS  +C+A   A E  L
Sbjct: 326 KAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKASERDL 385

Query: 361 QDGLNWACGQGGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMI 414
           +  L+WACG G  +C AIQP +PC+ P+ L +HAS+ FN Y+Q+  +    C F G  + 
Sbjct: 386 KGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGVK 445

[Close]

BLAST of MS000534 vs. ExPASy TrEMBL
Match: A0A6J1DG93 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020775 PE=3 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.4e-237
Identity = 410/413 (99.27%), Postives = 411/413 (99.52%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA
Sbjct: 85  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY
Sbjct: 145 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGI VVVT
Sbjct: 205 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPWFGG NEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG
Sbjct: 265 ETGWPWFGGGNEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           PVSEKNWGILFPNGSAVYPLG+TSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA
Sbjct: 325 PVSEKNWGILFPNGSAVYPLGVTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
           NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS
Sbjct: 385 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 437

[Close]

BLAST of MS000534 vs. ExPASy TrEMBL
Match: A0A6J1ENA7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434126 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 8.7e-219
Identity = 375/413 (90.80%), Postives = 392/413 (94.92%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVS LPSASD VAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSNLPSASDIVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAAYLP TNITAIAVGSEVL+ IP  GPVLVPA+YS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLSTIPQAGPVLVPALYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN ++KVSTPQSMD+IPR FPPSTATFNASWN+T+YQLLQFL NTKSYYMLNAYPY GY
Sbjct: 145 NLNYLVKVSTPQSMDMIPRPFPPSTATFNASWNATMYQLLQFLKNTKSYYMLNAYPYLGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGI VVVT
Sbjct: 205 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGIPVVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPW GGANEPDATIQNAGTY NNLIRRV+NDSGPPSQPTIPINTY+YELFNEDK+PG
Sbjct: 265 ETGWPWSGGANEPDATIQNAGTYVNNLIRRVSNDSGPPSQPTIPINTYIYELFNEDKRPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           PVSEKNWGILF NGS VYPLGL SGRATANSS VYCVAKDGADEDKLQDGLNWACGQGGA
Sbjct: 325 PVSEKNWGILFSNGSTVYPLGLASGRATANSSTVYCVAKDGADEDKLQDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
           NC AIQ GRPC+LPNN+T+HASYA+NDYYQKMH+AGGTCDFDGTA +TTIDPS
Sbjct: 385 NCAAIQQGRPCFLPNNITDHASYAYNDYYQKMHTAGGTCDFDGTATLTTIDPS 437

[Close]

BLAST of MS000534 vs. ExPASy TrEMBL
Match: A0A6J1H7X6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461352 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 5.7e-218
Identity = 374/419 (89.26%), Postives = 394/419 (94.03%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVS LPSAS  VAILK+H+ITHLRLYNAD+QLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTDVSNLPSASGIVAILKTHRITHLRLYNADSQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAAYLP TNITAIAVGSEVLTAIPHVGPVLVPAMYS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAYLPGTNITAIAVGSEVLTAIPHVGPVLVPAMYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN +IKVSTPQSMD+IPR FPPSTATFNASWNSTI QLLQFL NTKSYYMLNAYPYYGY
Sbjct: 145 NLNDLIKVSTPQSMDLIPRPFPPSTATFNASWNSTISQLLQFLKNTKSYYMLNAYPYYGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           T+ NGIFPLDYALFRSLPA+KQIVDPNTLFHYNSMFDAMVDATYYSID F FSGI +VVT
Sbjct: 205 TTENGIFPLDYALFRSLPAVKQIVDPNTLFHYNSMFDAMVDATYYSIDTFKFSGIPIVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPWFGGANEPDATIQNAGTY +NLIRRV+NDSGPPSQPT PINTYLYELFNEDK+PG
Sbjct: 265 ETGWPWFGGANEPDATIQNAGTYISNLIRRVSNDSGPPSQPTTPINTYLYELFNEDKRPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           P+SEKNWGILFPNGSAVYPLGL SGR+T NSSAVYCVAKDGADEDKL+DGLNWACGQGGA
Sbjct: 325 PISEKNWGILFPNGSAVYPLGLISGRSTGNSSAVYCVAKDGADEDKLEDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFFLLF 420
           NC AIQ GRPC+LPNN+TNHASYA+NDYYQK  SAGGTCDFDGTAM+TTIDPS    +F
Sbjct: 385 NCAAIQRGRPCFLPNNITNHASYAYNDYYQKSRSAGGTCDFDGTAMLTTIDPSHGSCIF 443

[Close]

BLAST of MS000534 vs. ExPASy TrEMBL
Match: A0A5A7UL74 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold319G00580 PE=3 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 6.3e-217
Identity = 375/416 (90.14%), Postives = 392/416 (94.23%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGT VS LPS SD VAILKSHQITHLRLYNAD QLLKALANSSIEVIVGVTNEEVL
Sbjct: 25  VGVNLGTSVSNLPSPSDIVAILKSHQITHLRLYNADPQLLKALANSSIEVIVGVTNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAA+LP TNITAIAVGSEVLT IPHVGPVLVPAMYS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAHLPGTNITAIAVGSEVLTTIPHVGPVLVPAMYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN +IKVSTPQSMDIIPR FPPSTA+F+ASWNSTIYQLLQFL NTKSYYMLNAYPYYGY
Sbjct: 145 NLNYLIKVSTPQSMDIIPRPFPPSTASFDASWNSTIYQLLQFLKNTKSYYMLNAYPYYGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           TSGNG FPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSI+AFNFS I VVVT
Sbjct: 205 TSGNGSFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIEAFNFSEIPVVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWP FGGANEPDATIQNAGTY +NLIRRV+NDSGPPSQPTIPINTYLYELFNEDK+PG
Sbjct: 265 ETGWPSFGGANEPDATIQNAGTYVSNLIRRVSNDSGPPSQPTIPINTYLYELFNEDKRPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           P+SEKNWGILFPNGSAVYPL L SGRATANSS VYCVAKDGADEDKLQDGLNWACGQGGA
Sbjct: 325 PISEKNWGILFPNGSAVYPLSLMSGRATANSSEVYCVAKDGADEDKLQDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFF 417
           NC AIQ GRPC+LPNN+T+HASYA+NDYYQKM  AGGTCDFDGTA +TT+DPSK+F
Sbjct: 385 NCAAIQQGRPCFLPNNITDHASYAYNDYYQKMRGAGGTCDFDGTATLTTVDPSKWF 440

[Close]

BLAST of MS000534 vs. ExPASy TrEMBL
Match: A0A6J1JL91 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485438 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 8.2e-217
Identity = 373/419 (89.02%), Postives = 394/419 (94.03%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           VGVNLGTDVS LPSASD VAILK+H ITHLRLYNAD+QLLKALANSSIEVIVGV NEEVL
Sbjct: 25  VGVNLGTDVSNLPSASDIVAILKTHWITHLRLYNADSQLLKALANSSIEVIVGVMNEEVL 84

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           RIGESPA AAAWVNKNVAA+LP TNITAIAVGSEVLTAIPHVGPVLVPAMYS+HKALVAA
Sbjct: 85  RIGESPAAAAAWVNKNVAAHLPGTNITAIAVGSEVLTAIPHVGPVLVPAMYSLHKALVAA 144

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN +IKVSTPQSMD+IPR FPPSTATFNASWNSTI QLLQFL NTKSYYMLNAYPYYGY
Sbjct: 145 NLNDLIKVSTPQSMDLIPRPFPPSTATFNASWNSTISQLLQFLKNTKSYYMLNAYPYYGY 204

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           T+ NGIFPLDYALFRSLPA+KQIVDPNTLFHYNSMFDAMVDATYYSI AF FSGI +VVT
Sbjct: 205 TTENGIFPLDYALFRSLPAVKQIVDPNTLFHYNSMFDAMVDATYYSIYAFKFSGIPIVVT 264

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWPWFGGANEPDATIQNAGTY +NLIRRV+NDSGPPSQPT PINTYLYELFNEDK+PG
Sbjct: 265 ETGWPWFGGANEPDATIQNAGTYISNLIRRVSNDSGPPSQPTTPINTYLYELFNEDKRPG 324

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATANSSAVYCVAKDGADEDKLQDGLNWACGQGGA 360
           P+SEKNWGILFPNGSAVYPLGL SGR+TANSSAVYCVAKDGADEDKL+DGLNWACGQGGA
Sbjct: 325 PISEKNWGILFPNGSAVYPLGLISGRSTANSSAVYCVAKDGADEDKLEDGLNWACGQGGA 384

Query: 361 NCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFFLLF 420
           NC AIQ GRPC+LPN++TNHASYA+NDYYQK HSAGGTCDFDGTAM+T IDPS    +F
Sbjct: 385 NCAAIQRGRPCFLPNSITNHASYAYNDYYQKTHSAGGTCDFDGTAMLTAIDPSHGSCIF 443

[Close]

BLAST of MS000534 vs. TAIR 10
Match: AT3G13560.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 621.3 bits (1601), Expect = 5.7e-178
Identity = 295/415 (71.08%), Postives = 357/415 (86.02%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GTD++ +P  SD V +LKS QITH+RLY+A++ +LKA AN+SIEV+VGVTNEE+L
Sbjct: 26  IGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTSIEVMVGVTNEEIL 85

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           +IG  P+ AAAWVNKNVAAY+P+TNITAIAVGSEVLT IPHV P+L  A+ +IHKALVA+
Sbjct: 86  KIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILASALNNIHKALVAS 145

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN  +KVS+P SMDI+P+ FPPST+TF+ SWN+T+YQLLQFL NT S++MLNAYPYYGY
Sbjct: 146 NLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTGSFFMLNAYPYYGY 205

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           T+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA YYS++A NFS I VVVT
Sbjct: 206 TTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVVT 265

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWP  GG++E  AT+ NA T+  NLI+RV N+SGPPSQP IPINTY+YEL+NEDK+ G
Sbjct: 266 ETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINTYIYELYNEDKRSG 325

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATA--NSSAVYCVAKDGADEDKLQDGLNWACGQG 360
           PVSE+NWGILFPNG++VYPL L+ G ++A  N S+++CVAK  AD+DKL DGLNWACGQG
Sbjct: 326 PVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKADADDDKLVDGLNWACGQG 385

Query: 361 GANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
            ANC AIQPG+PCYLPN++ +HAS+AFNDYYQKM SAGGTCDFDGTA+ TT DPS
Sbjct: 386 RANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of MS000534 vs. TAIR 10
Match: AT3G13560.3 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 621.3 bits (1601), Expect = 5.7e-178
Identity = 295/415 (71.08%), Postives = 357/415 (86.02%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GTD++ +P  SD V +LKS QITH+RLY+A++ +LKA AN+SIEV+VGVTNEE+L
Sbjct: 26  IGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTSIEVMVGVTNEEIL 85

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           +IG  P+ AAAWVNKNVAAY+P+TNITAIAVGSEVLT IPHV P+L  A+ +IHKALVA+
Sbjct: 86  KIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILASALNNIHKALVAS 145

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN  +KVS+P SMDI+P+ FPPST+TF+ SWN+T+YQLLQFL NT S++MLNAYPYYGY
Sbjct: 146 NLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTGSFFMLNAYPYYGY 205

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           T+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA YYS++A NFS I VVVT
Sbjct: 206 TTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVVT 265

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWP  GG++E  AT+ NA T+  NLI+RV N+SGPPSQP IPINTY+YEL+NEDK+ G
Sbjct: 266 ETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINTYIYELYNEDKRSG 325

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATA--NSSAVYCVAKDGADEDKLQDGLNWACGQG 360
           PVSE+NWGILFPNG++VYPL L+ G ++A  N S+++CVAK  AD+DKL DGLNWACGQG
Sbjct: 326 PVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKADADDDKLVDGLNWACGQG 385

Query: 361 GANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
            ANC AIQPG+PCYLPN++ +HAS+AFNDYYQKM SAGGTCDFDGTA+ TT DPS
Sbjct: 386 RANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of MS000534 vs. TAIR 10
Match: AT3G13560.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 621.3 bits (1601), Expect = 5.7e-178
Identity = 295/415 (71.08%), Postives = 357/415 (86.02%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GTD++ +P  SD V +LKS QITH+RLY+A++ +LKA AN+SIEV+VGVTNEE+L
Sbjct: 26  IGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTSIEVMVGVTNEEIL 85

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
           +IG  P+ AAAWVNKNVAAY+P+TNITAIAVGSEVLT IPHV P+L  A+ +IHKALVA+
Sbjct: 86  KIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILASALNNIHKALVAS 145

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NLN  +KVS+P SMDI+P+ FPPST+TF+ SWN+T+YQLLQFL NT S++MLNAYPYYGY
Sbjct: 146 NLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTGSFFMLNAYPYYGY 205

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
           T+ NGIFPLDYALF+ L  +KQIVDPNTL HYNSMFDAMVDA YYS++A NFS I VVVT
Sbjct: 206 TTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVVT 265

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           ETGWP  GG++E  AT+ NA T+  NLI+RV N+SGPPSQP IPINTY+YEL+NEDK+ G
Sbjct: 266 ETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINTYIYELYNEDKRSG 325

Query: 301 PVSEKNWGILFPNGSAVYPLGLTSGRATA--NSSAVYCVAKDGADEDKLQDGLNWACGQG 360
           PVSE+NWGILFPNG++VYPL L+ G ++A  N S+++CVAK  AD+DKL DGLNWACGQG
Sbjct: 326 PVSERNWGILFPNGTSVYPLSLSGGSSSAALNGSSMFCVAKADADDDKLVDGLNWACGQG 385

Query: 361 GANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
            ANC AIQPG+PCYLPN++ +HAS+AFNDYYQKM SAGGTCDFDGTA+ TT DPS
Sbjct: 386 RANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of MS000534 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 437.2 bits (1123), Expect = 1.5e-122
Identity = 217/422 (51.42%), Postives = 292/422 (69.19%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GT+V+ +PS +  VA+LKS  I  +RLY+AD  +L A A++ ++VI+ V N+++L
Sbjct: 23  IGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFAHTGVQVIISVPNDQLL 82

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
            I +S A AA WV +NVAAY PATNIT IAVGSEVLT++ +   VLV A+  I  ALV A
Sbjct: 83  GISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAASVLVSALKYIQAALVTA 142

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NL+  IKVSTP S  II  +FPPS A FN +W+  I  LL+FL +T S  +LN YPY+ Y
Sbjct: 143 NLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFLQSTGSPLLLNVYPYFDY 202

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
              NG+ PLDYALF+ L A K+ VD NTL HY ++FDA+VDA Y+++   NF+ I +VVT
Sbjct: 203 VQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAYFAMSYLNFTNIPIVVT 262

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           E+GWP  GG +E DAT++NA TY +NLI+ V N +G P  P   + TY+YEL+NED +PG
Sbjct: 263 ESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTAVTTYIYELYNEDTRPG 322

Query: 301 PVSEKNWGILFPNGSAVYPLGLT-SGRATAN--SSAVYCVAKDGADEDKLQDGLNWACGQ 360
           PVSEKNWG+ + NG+ VY L L  +G   AN  ++  +C+AK+  D   LQ  L+WACG 
Sbjct: 323 PVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKEKVDRKMLQAALDWACGP 382

Query: 361 GGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPSKFFL 420
           G  +C A+  G  CY P+++  H++YAFN YYQKM  A G+CDF G A +TT DPS+   
Sbjct: 383 GKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATVTTTDPSRGTC 442

[Close]

BLAST of MS000534 vs. TAIR 10
Match: AT1G66250.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.4e-120
Identity = 218/416 (52.40%), Postives = 291/416 (69.95%), Query Frame = 0


Query: 1   VGVNLGTDVSKLPSASDTVAILKSHQITHLRLYNADAQLLKALANSSIEVIVGVTNEEVL 60
           +GVN+GTD+S +P  +  VA+LK+ +I H+RLYNAD  LL ALAN+ I+VI+ + N+++L
Sbjct: 32  IGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQLL 91

Query: 61  RIGESPAVAAAWVNKNVAAYLPATNITAIAVGSEVLTAIPHVGPVLVPAMYSIHKALVAA 120
            IG+S + AA WV +NV A+ PAT ITA++VGSEVLT++ +  PVLV A+ ++H AL++A
Sbjct: 92  GIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALLSA 151

Query: 121 NLNSMIKVSTPQSMDIIPRAFPPSTATFNASWNSTIYQLLQFLNNTKSYYMLNAYPYYGY 180
           NL+ +IKVSTP S  +I   FPPS A FN S N+ I  LL FL +T SY M+N YPY  Y
Sbjct: 152 NLDKLIKVSTPLSTSLILDPFPPSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYIDY 211

Query: 181 TSGNGIFPLDYALFRSLPAIKQIVDPNTLFHYNSMFDAMVDATYYSIDAFNFSGITVVVT 240
              NG+ PLDYALF+ +P  K+ VD NTL  Y++ FDAMVDATY+++   NF+ I V+VT
Sbjct: 212 MQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVT 271

Query: 241 ETGWPWFGGANEPDATIQNAGTYCNNLIRRVTNDSGPPSQPTIPINTYLYELFNEDKKPG 300
           E+GWP  G  NEPDAT+ NA TY +NLIR V N +G P +P I ++TY+YEL+NED K G
Sbjct: 272 ESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTKAG 331

Query: 301 PVSEKNWGILFPNGSAVYPLGLT-SGRATAN--SSAVYCVAKDGADEDKLQDGLNWACGQ 360
            +SEKNWG+   NG  VY L LT SG   AN  ++  YC A++GAD   LQ  L+WACG 
Sbjct: 332 -LSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTAREGADTKMLQAALDWACGP 391

Query: 361 GGANCVAIQPGRPCYLPNNLTNHASYAFNDYYQKMHSAGGTCDFDGTAMITTIDPS 414
           G  +C  I+ G  CY P+N+  HA+YAF+ YY +  +    C+F+G A ITT DPS
Sbjct: 392 GKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPS 446

The following BLAST results are available for this feature:

BLAST of MS000534 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153235.1	5.1e-237	99.27	glucan endo-1,3-beta-glucosidase 4-like [Momordica charantia] >XP_022153236.1 gl...	[more]
XP_038879038.1	4.3e-220	90.93	glucan endo-1,3-beta-glucosidase 4-like [Benincasa hispida] >XP_038879039.1 gluc...	[more]
KAG7023810.1	9.6e-220	90.38	Glucan endo-1,3-beta-glucosidase 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG7022153.1	6.2e-219	91.04	Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022927211.1	1.8e-218	90.80	glucan endo-1,3-beta-glucosidase 4-like [Cucurbita moschata] >XP_022927220.1 glu...	[more]

BLAST of MS000534 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94CD8	8.0e-177	71.08	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
Q9ZU91	2.1e-121	51.42	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9C7U5	2.0e-119	52.40	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
O65399	5.4e-117	50.95	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9FJU9	2.9e-86	40.14	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of MS000534 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DG93	2.4e-237	99.27	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020775 ...	[more]
A0A6J1ENA7	8.7e-219	90.80	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434126 P...	[more]
A0A6J1H7X6	5.7e-218	89.26	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461352 P...	[more]
A0A5A7UL74	6.3e-217	90.14	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A6J1JL91	8.2e-217	89.02	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485438 PE=...	[more]

BLAST of MS000534 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13560.1	5.7e-178	71.08	O-Glycosyl hydrolases family 17 protein	[more]
AT3G13560.3	5.7e-178	71.08	O-Glycosyl hydrolases family 17 protein	[more]
AT3G13560.2	5.7e-178	71.08	O-Glycosyl hydrolases family 17 protein	[more]
AT2G01630.1	1.5e-122	51.42	O-Glycosyl hydrolases family 17 protein	[more]
AT1G66250.1	1.4e-120	52.40	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 334..415 e-value: 4.8E-44 score: 162.3
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 335..406 e-value: 6.7E-21 score: 74.8
None	No IPR available	GENE3D	1.20.58.1040		coord: 329..415 e-value: 2.1E-10 score: 42.8
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 1..328 e-value: 3.3E-108 score: 363.4
None	No IPR available	PANTHER	PTHR32227:SF274	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 4	coord: 1..414
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 1..320 e-value: 1.1E-78 score: 265.0
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 1..414
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..320

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000534.1	MS000534.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds