MS000511 (gene) Bitter gourd (TR) v1

Overview
NameMS000511
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionreticulocalbin-2
Locationscaffold44: 2162995 .. 2164996 (-)
RNA-Seq ExpressionMS000511
SyntenyMS000511
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAGGGCGGTGGTTTATCTTTTTCTAATCACCGCCTTCGCCTTCTTCTTCGTATTTTATCCGTCCAATTCCCCCGATCGGAACCATCAAGAACTAACTCGTCGCCTAGGTTACAAATTACGAGCCCCTACTTTTGATCCTCTTGTTGAAGAAATGGAAAGATACGCAGAGGAACATGGATCAAGCAGGGGACGAAACGCCATTAGTGGGCAAAGAACTACCACGGCCAATCAGGTTCCCGATGCTTACAAAGATTACTACGATGAAGGTAGATTGAAAATTTCGTTGAGGCTGCTCGTGTTGTTTCCTTTGCTGGACCACTCGCCAAAAGACGGTGTTATCAGTTACGAGGAGTTGAGCGATTGGATTATTGAACAAGCCGTGCAGAGATTGAATTACAGGACACGGAAAGAGTTGGACTTCCATGACAAGAATGGAGATGGCGCCATTTCATTCCGCGAATATCTGCCACAGTTTACAGAGGAAGATATTGGTATGCCTTTGATGGCCTTCTTTTCTTTTGATAGCTAGGAAAGTTCGTTGTAGACATTCAATTTTCTGTCCACAATTTCCTGGAAATTTGATAATTTTTAGGTTGAAATGTTACTGATAATAATGATAGATAAAAACAAATGGGATGGAATATGGAGAACCTGAAGAGTCTATTATTATTTTCTTCCCTTCATTGCTCTGTTTTCATGGTAGCCAAACACATTTTGGGATTGTGATTGAAACTACTGAACATTATGAAACAGCAAAGAGAGGAACGGCGCATGGAGAAGCTGGATGGTGGAAAAGGCAATTCACAAATGCTGATACAGACCCAAATGGATTACTAAATTTTGAAGAACTGAAAGAGTATGTGCCATCTTCCCTCCCAGAAAAAAAACAGTGTACTTTACTATTTTTAGCCTTTTGTTTCTTGTAGTTTCATCTGTATCATTTTTGAATTTAATGTTTCAATTCAGTTTCTACTGTTAATTATCCAGTTAAATAGTAACAATAAATTGTTGTAGAAGCTAGCATGATGCTACATGGACTATTGCGTTATATGTCACGATTTAACCTATCAAAGGTGTGAAGTCAGGTTCCATGTTTTAGCTCTTTTTCTCAATCATTATTTAACTGGATAATTAACCATAAAGACTAAATGGATATGTCTATAAAAGTATTGTGATTAAAATGAAACAATGAAATTACATAGATGAAAATGTTAAAAATGAGAGTACACAGACTAGAATTGTAGTTAATTTGATGAAACACGTGTTTCCTGACAAGAATGGAATGAGATCTTTCTGTCCTGCAGTTTCCTGCACCCAGAAGATAGCAGCAATTACCGGATACAAAATTGGCTCTTGCGAGAGAAAATGAAGTAATTATTCCACATAGTCAAAAGCTATAAACTTTTTCTCATCTGCATGTGAACCAAAGCATCAGCTTCTGTTTAATATGCAGGCGCATGGACTATGACAGAGATGGAAAACTCAATTTTGATGAATTCCTCCACCATGTATTTGACATATACAAGAGCTACATTGAATTTGAAACTCAAGGGGATGATGTACCGACAGCCGAAGAGAAGTTTGATGAGCTTGATCTTGACGAGGATGAGTATGCATTTGTTTTATGTCGAAGAGCCATTTTTTTTTCCCTTAATCCCAATTTGTGTGGTGTTTTCAATATCAAACAATGCTGTGTAGGTTACTGTCAGTGGAAGAGTTGAGGCCATTGTTCCAGTATCTTCATCCTGGAGAACTGTCTTATGCCCAACATTACACAAGTCATTTGATATATGAGGTAATAGTAGCTGATTTGAAAAGATGAAGAGATCAAACATGATGAAGAAATTAGCTGATTTTGTGAATCAATACTGCAGGCTGATGATAACAAAGATGGCTACTTATCACTTGATGAGATGCTCAATCATGAGTATGTTTTCTACAGCTCGGTATATGAAGGTCGCAATGGCGACTATGAAGACGATTACCATGATGAACTT

mRNA sequence

ATGGGGAGGGCGGTGGTTTATCTTTTTCTAATCACCGCCTTCGCCTTCTTCTTCGTATTTTATCCGTCCAATTCCCCCGATCGGAACCATCAAGAACTAACTCGTCGCCTAGGTTACAAATTACGAGCCCCTACTTTTGATCCTCTTGTTGAAGAAATGGAAAGATACGCAGAGGAACATGGATCAAGCAGGGGACGAAACGCCATTAGTGGGCAAAGAACTACCACGGCCAATCAGGTTCCCGATGCTTACAAAGATTACTACGATGAAGGTAGATTGAAAATTTCGTTGAGGCTGCTCGTGTTGTTTCCTTTGCTGGACCACTCGCCAAAAGACGGTGTTATCAGTTACGAGGAGTTGAGCGATTGGATTATTGAACAAGCCGTGCAGAGATTGAATTACAGGACACGGAAAGAGTTGGACTTCCATGACAAGAATGGAGATGGCGCCATTTCATTCCGCGAATATCTGCCACAGTTTACAGAGGAAGATATTGCAAAGAGAGGAACGGCGCATGGAGAAGCTGGATGGTGGAAAAGGCAATTCACAAATGCTGATACAGACCCAAATGGATTACTAAATTTTGAAGAACTGAAAGATTTCCTGCACCCAGAAGATAGCAGCAATTACCGGATACAAAATTGGCTCTTGCGAGAGAAAATGAAGCGCATGGACTATGACAGAGATGGAAAACTCAATTTTGATGAATTCCTCCACCATGTATTTGACATATACAAGAGCTACATTGAATTTGAAACTCAAGGGGATGATGTACCGACAGCCGAAGAGAAGTTTGATGAGCTTGATCTTGACGAGGATGAGTTACTGTCAGTGGAAGAGTTGAGGCCATTGTTCCAGTATCTTCATCCTGGAGAACTGTCTTATGCCCAACATTACACAAGTCATTTGATATATGAGGCTGATGATAACAAAGATGGCTACTTATCACTTGATGAGATGCTCAATCATGAGTATGTTTTCTACAGCTCGGTATATGAAGGTCGCAATGGCGACTATGAAGACGATTACCATGATGAACTT

Coding sequence (CDS)

ATGGGGAGGGCGGTGGTTTATCTTTTTCTAATCACCGCCTTCGCCTTCTTCTTCGTATTTTATCCGTCCAATTCCCCCGATCGGAACCATCAAGAACTAACTCGTCGCCTAGGTTACAAATTACGAGCCCCTACTTTTGATCCTCTTGTTGAAGAAATGGAAAGATACGCAGAGGAACATGGATCAAGCAGGGGACGAAACGCCATTAGTGGGCAAAGAACTACCACGGCCAATCAGGTTCCCGATGCTTACAAAGATTACTACGATGAAGGTAGATTGAAAATTTCGTTGAGGCTGCTCGTGTTGTTTCCTTTGCTGGACCACTCGCCAAAAGACGGTGTTATCAGTTACGAGGAGTTGAGCGATTGGATTATTGAACAAGCCGTGCAGAGATTGAATTACAGGACACGGAAAGAGTTGGACTTCCATGACAAGAATGGAGATGGCGCCATTTCATTCCGCGAATATCTGCCACAGTTTACAGAGGAAGATATTGCAAAGAGAGGAACGGCGCATGGAGAAGCTGGATGGTGGAAAAGGCAATTCACAAATGCTGATACAGACCCAAATGGATTACTAAATTTTGAAGAACTGAAAGATTTCCTGCACCCAGAAGATAGCAGCAATTACCGGATACAAAATTGGCTCTTGCGAGAGAAAATGAAGCGCATGGACTATGACAGAGATGGAAAACTCAATTTTGATGAATTCCTCCACCATGTATTTGACATATACAAGAGCTACATTGAATTTGAAACTCAAGGGGATGATGTACCGACAGCCGAAGAGAAGTTTGATGAGCTTGATCTTGACGAGGATGAGTTACTGTCAGTGGAAGAGTTGAGGCCATTGTTCCAGTATCTTCATCCTGGAGAACTGTCTTATGCCCAACATTACACAAGTCATTTGATATATGAGGCTGATGATAACAAAGATGGCTACTTATCACTTGATGAGATGCTCAATCATGAGTATGTTTTCTACAGCTCGGTATATGAAGGTCGCAATGGCGACTATGAAGACGATTACCATGATGAACTT

Protein sequence

MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEHGSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYTSHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL
Homology
BLAST of MS000511 vs. NCBI nr
Match: XP_022141310.1 (reticulocalbin-2 [Momordica charantia])

HSP 1 Score: 713.4 bits (1840), Expect = 9.7e-202
Identity = 345/347 (99.42%), Postives = 346/347 (99.71%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH
Sbjct: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
           GS RGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL
Sbjct: 61  GSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR
Sbjct: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH
Sbjct: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
           VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT
Sbjct: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           SHLIYEADDNKDGYLSLDEMLNHEYVFY+SVYEGRNGDYEDDYHDEL
Sbjct: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDDYHDEL 347

BLAST of MS000511 vs. NCBI nr
Match: XP_022945265.1 (reticulocalbin-2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 262/347 (75.50%), Postives = 303/347 (87.32%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MGRAVVY+ ++ AFA FFVF PS+ PDRN QELTRRLGYK R PTFDPLV E+ER AEE 
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
                 NAI  ++T  A ++P+AY+D  DEGRL IS RL+VLFPL+DHSP DG+I+YEE+
Sbjct: 61  ELGSRGNAIGVEQTAKA-KIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEM 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           S+WI EQ V +LN RT +EL F+DKNGDGAISF EYLPQFTEEDIA+  T HG+AGWWK+
Sbjct: 121 SNWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD DPNGLL+F+ELKDFLHP+DSSNYRIQNWLL EKMKRMDYDRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            +DIYKSYIEFETQGDDVPTAEEKFDELDLDEDE+L+VEELRPLFQYL+PGELS+AQHY 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           +HLI EADDNKDG+L+L+EML HEYVFY+++YE +NGDYEDD+HDEL
Sbjct: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of MS000511 vs. NCBI nr
Match: XP_023541445.1 (reticulocalbin-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 549.3 bits (1414), Expect = 2.4e-152
Identity = 261/347 (75.22%), Postives = 302/347 (87.03%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MGRAVVY+ +  AFA FFVF PS+ PDRN QELTRRLGYK R PTFDPLV E+ER AEE 
Sbjct: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
                 N I  ++T  A ++P+AY+D  DEGRL IS RL+VLFPL+DHSPKDG+I+YEE+
Sbjct: 61  ELGSRGNVIGVEQTAKA-KIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEM 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           S+WI EQ + +LN RT +EL F+DKNGDGAISF EYLPQFTEEDIA+  T HG+AGWWK+
Sbjct: 121 SNWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD DPNGLL+F+ELKDFLHP+DSSNYRIQNWLL EKMKRMDYDRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            +DIYKSYIEFETQGDDVPTAEEKFDELDLDEDE+L+VEELRPLFQYL+PGELS+AQHY 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           +HLI EADDNKDG+L+L+EML HEYVFY+++YE +NGDYEDD+HDEL
Sbjct: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of MS000511 vs. NCBI nr
Match: KAG7013311.1 (45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 548.5 bits (1412), Expect = 4.1e-152
Identity = 261/347 (75.22%), Postives = 303/347 (87.32%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MGRAVVY+ ++ AFA FFVF PS+ PDRN QELTRRLGY+ R PTFDPLV E+ER AEE 
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYRFRTPTFDPLVAELERIAEER 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
                 NAI  ++T  A ++P+AY+D  DEGRL IS RL+VLFPL+DHSP DG+I+YEE+
Sbjct: 61  ELGSRGNAIGVEQTAKA-KIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEM 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           S+WI EQ V +LN RT +EL F+DKNGDGAISF EYLPQFTEEDIA+  T HG+AGWWK+
Sbjct: 121 SNWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD DPNGLL+F+ELKDFLHP+DSSNYRIQNWLL EKMKRMDYDRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            +DIYKSYIEFETQGDDVPTAEEKFDELDLDEDE+L+VEELRPLFQYL+PGELS+AQHY 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           +HLI EADDNKDG+L+L+EML HEYVFY+++YE +NGDYEDD+HDEL
Sbjct: 301 AHLIKEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of MS000511 vs. NCBI nr
Match: KAG6574244.1 (45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548.1 bits (1411), Expect = 5.4e-152
Identity = 261/347 (75.22%), Postives = 303/347 (87.32%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MGRAVVY+ ++ AFA FFVF PS+ PDRN QELTRRLGYK R PTFDPLV E+ER AEE 
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
                 NAI  ++T  A ++P+AY+D  DEGRL IS RL+VLFPL+DHSP DG+I+YEE+
Sbjct: 61  ELGSRGNAIGVEQTAKA-KIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEM 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           S+WI EQ V +LN RT +EL F+DKNGDGAISF EYLPQFTEEDIA+  T HG+AGWWK+
Sbjct: 121 SNWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD DPNGLL+F+ELKDFLHP+DSSNYRIQNWLL EKMKRMDYDRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            +DIYKSYIEFETQGDDVPTAEEKFDELDLDEDE+L+VEELRPLFQYL+PGELS+AQH+ 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHFA 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           +HLI EADDNKDG+L+L+EML HEYVFY+++YE +NGDYEDD+HDEL
Sbjct: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of MS000511 vs. ExPASy Swiss-Prot
Match: B5X186 (Calumenin-A OS=Salmo salar OX=8030 GN=calua PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 3.2e-14
Identity = 78/284 (27.46%), Postives = 134/284 (47.18%), Query Frame = 0

Query: 57  AEEHGSSRG----RNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKD 116
           + EH  ++G     +A  GQ+           K + D    +   RL V+   +D    D
Sbjct: 36  SREHDDAQGFDYDHDAFLGQKEA---------KSFDDLSPEESKRRLGVIVEKID-GDSD 95

Query: 117 GVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREY--LPQFTEEDIAKRGT 176
           G ++  EL  WI +   + +     ++    D N DG IS+ EY  +   T  D  +   
Sbjct: 96  GFVTEVELRAWIKKAQKKYIYENVDRQWKDFDVNNDGMISWEEYRNVTYGTYLDDPEPDD 155

Query: 177 AHGEAGWW---KRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYD 236
            +         +R+F  AD + + + N EE   FLHPE+  +  +++ ++ E M+ +D +
Sbjct: 156 GYNYQHMMARDERRFKMADQNRDQIANKEEFTAFLHPEEYDH--MKDIVVLETMEDIDKN 215

Query: 237 RDGKLNFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKFDEL-DLDEDELLSVEELRPLFQ 296
            DG ++ +E+   + D+Y    E E + D V T  E+F E  D ++D  +  EE      
Sbjct: 216 GDGFIDLNEY---IGDMYNHEDEME-EPDWVATEREQFSEFRDKNKDGKMDREE---TMD 275

Query: 297 YLHPGELSYAQHYTSHLIYEADDNKDGYLSLDEMLNHEYVFYSS 331
           ++ P +  +A+    HL+YE+D NKDG LS +E+LN   +F  S
Sbjct: 276 WILPSDYDHAEAEAKHLVYESDSNKDGKLSKEEILNKYDLFVGS 300

BLAST of MS000511 vs. ExPASy Swiss-Prot
Match: B5X4E0 (Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.2e-14
Identity = 65/239 (27.20%), Postives = 123/239 (51.46%), Query Frame = 0

Query: 98  RLLVLFPLLDHSPKDGVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREY- 157
           RL +L   +D   KDG +S EE+  WI     + +     ++   HD NGDG +S+ EY 
Sbjct: 73  RLGMLVERIDED-KDGYVSVEEMKKWIKHSQKRWIYDDVDRQWKGHDHNGDGLVSWEEYK 132

Query: 158 ---LPQFTEEDIAKRGTAHGE-AGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRI 217
                   ++   + G ++ +     +R+F  +D D +   N EE   FLHPE+  +  +
Sbjct: 133 NATYGYILDDPDPEDGFSYRQMISRDERRFKMSDLDADLKANKEEFTAFLHPEEYDH--M 192

Query: 218 QNWLLREKMKRMDYDRDGKLNFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKFDEL-DLD 277
           ++ ++ E M+ +D + DG ++ +E+   + D+Y    +  ++ + V T  E+F E  D +
Sbjct: 193 KDIVVLETMEDIDKNGDGFIDLEEY---IGDMYNQEGD-PSEPEWVRTEREQFTEFRDTN 252

Query: 278 EDELLSVEELRPLFQYLHPGELSYAQHYTSHLIYEADDNKDGYLSLDEMLNHEYVFYSS 331
           +D  +  EE +    ++ P +  +A+    HL+YE+D++KDG L+  E++    +F  S
Sbjct: 253 KDGRMDKEETK---DWILPSDYDHAEAEAKHLVYESDNDKDGKLTKAEIVEKYDLFVGS 301

BLAST of MS000511 vs. ExPASy Swiss-Prot
Match: Q7ZUC2 (45 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.6e-13
Identity = 73/270 (27.04%), Postives = 135/270 (50.00%), Query Frame = 0

Query: 98  RLLVLFPLLDHSPKDGVISYEELSDWIIEQAVQRLNYRTRK-ELDFH--DKNGDGAISFR 157
           +L+ +F  +D + KD  +S +E+  WI+E+  +      R+ +L F   D +GDG +++ 
Sbjct: 96  KLIEIFTKVDIN-KDRSVSAKEMQRWIMEKTEEHFQEAVRENKLSFRAVDPDGDGLVTWD 155

Query: 158 EYLPQF------TEEDIAKRGTAHGE----------AGWWKRQFTNADTDP-NGLLNFEE 217
           EY  +F       E+++A++   + E              K ++  AD  P + LLN EE
Sbjct: 156 EYRVKFLASKGLNEKEVAEKIKNNEELKVDEETQEVLESLKDRWFQADNPPADQLLNEEE 215

Query: 218 LKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHHVFDIYKSYIEFETQGDD 277
              FLHPE S    +  ++++E ++ +D D DGKL   EF+       ++    +   D 
Sbjct: 216 FLSFLHPEHSRG--MLRYMVKEIVRDLDQDGDGKLTLAEFISLPMGTVENQQAQDIDDDW 275

Query: 278 VPTAEEKFDE-LDLDEDELLSVEELRPLFQYLHPGELSYAQHYTSHLIYEADDNKDGYLS 337
           V   +++F+E +D + D ++++EEL    +Y+ P     A +    +I  AD+N++  L 
Sbjct: 276 VRERKKEFEEVIDANHDTIVTMEELE---EYMDPMNEHNALNEAKQMIAVADENQNHNLE 335

Query: 338 LDEMLNHEYVFYSSVYEGRNGDYEDDYHDE 347
           L+E+L +   F  S    +  DY  + H+E
Sbjct: 336 LEEILKYSEYFTGS----KLMDYARNVHEE 355

BLAST of MS000511 vs. ExPASy Swiss-Prot
Match: J3S9D9 (Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 4.6e-13
Identity = 73/263 (27.76%), Postives = 126/263 (47.91%), Query Frame = 0

Query: 98  RLLVLFPLLDHSPKDGVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYL 157
           RL V+   +D    DG ++  ELS WI       +    +++   +DK+GDG +S+ EY 
Sbjct: 54  RLKVIISRID-VDLDGFLTEAELSSWIQHSFKSYIIEDAKQQFQHYDKDGDGRVSWEEYN 113

Query: 158 PQF----------TEEDIAKRGTAHGEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDS 217
            Q           T  D A+  +        K++F  A+ D +  L+FEE   F HPE++
Sbjct: 114 IQMYDRVIDFEEDTTLDDAEEESFRQLHLKDKKRFQKANKDGDSHLDFEEFAAFEHPEEA 173

Query: 218 SNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKF-D 277
               ++ ++++E ++  D D DG ++  EFL      Y+     +   + +   E++F +
Sbjct: 174 D--YMKEFVIQESLEEHDKDGDGFVSLQEFLGD----YRRDPAAKEDPEWIVVEEDRFKN 233

Query: 278 ELDLDEDELLSVEELRPLFQYLHPGELSYAQHYTSHLIYEADDNKDGYLSLDEMLNHEYV 337
           + D D+D  LS +E   L  ++ P     AQ    HL+ E D + D  LS +E+L ++ +
Sbjct: 234 DYDKDKDGKLSPKE---LLTWVMPNNEGLAQEEAVHLLDEMDLDGDRRLSANEILENQDL 293

Query: 338 FYSS--VYEGRNGDYEDDYHDEL 348
           F +S     GR    +  YH+EL
Sbjct: 294 FLNSEATDYGRQLHDKSFYHEEL 306

BLAST of MS000511 vs. ExPASy Swiss-Prot
Match: O43852 (Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 6.0e-13
Identity = 64/231 (27.71%), Postives = 114/231 (49.35%), Query Frame = 0

Query: 111 KDGVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYL----------PQF 170
           KDG ++ +EL DWI     + +     ++   HD N DG +S+ EY           P  
Sbjct: 84  KDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEYKNATYGYVLDDPDP 143

Query: 171 TEEDIAKRGTAHGEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREK 230
            +    K+     E     R+F  AD D + +   EE   FLHPE+     +++ +++E 
Sbjct: 144 DDGFNYKQMMVRDE-----RRFKMADKDGDLIATKEEFTAFLHPEEYD--YMKDIVVQET 203

Query: 231 MKRMDYDRDGKLNFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKFDEL-DLDEDELLSVE 290
           M+ +D + DG ++ +E+   + D+Y S+     + + V T  E+F E  D + D  +  E
Sbjct: 204 MEDIDKNADGFIDLEEY---IGDMY-SHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKE 263

Query: 291 ELRPLFQYLHPGELSYAQHYTSHLIYEADDNKDGYLSLDEMLNHEYVFYSS 331
           E +    ++ P +  +A+    HL+YE+D NKDG L+ +E+++   +F  S
Sbjct: 264 ETK---DWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLFVGS 300

BLAST of MS000511 vs. ExPASy TrEMBL
Match: A0A6J1CHQ2 (reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 4.7e-202
Identity = 345/347 (99.42%), Postives = 346/347 (99.71%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH
Sbjct: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
           GS RGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL
Sbjct: 61  GSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR
Sbjct: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH
Sbjct: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
           VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT
Sbjct: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           SHLIYEADDNKDGYLSLDEMLNHEYVFY+SVYEGRNGDYEDDYHDEL
Sbjct: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDDYHDEL 347

BLAST of MS000511 vs. ExPASy TrEMBL
Match: A0A6J1G0G2 (reticulocalbin-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449565 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.9e-153
Identity = 262/347 (75.50%), Postives = 303/347 (87.32%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MGRAVVY+ ++ AFA FFVF PS+ PDRN QELTRRLGYK R PTFDPLV E+ER AEE 
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
                 NAI  ++T  A ++P+AY+D  DEGRL IS RL+VLFPL+DHSP DG+I+YEE+
Sbjct: 61  ELGSRGNAIGVEQTAKA-KIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEM 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           S+WI EQ V +LN RT +EL F+DKNGDGAISF EYLPQFTEEDIA+  T HG+AGWWK+
Sbjct: 121 SNWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD DPNGLL+F+ELKDFLHP+DSSNYRIQNWLL EKMKRMDYDRDGKL+FDEFLHH
Sbjct: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            +DIYKSYIEFETQGDDVPTAEEKFDELDLDEDE+L+VEELRPLFQYL+PGELS+AQHY 
Sbjct: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           +HLI EADDNKDG+L+L+EML HEYVFY+++YE +NGDYEDD+HDEL
Sbjct: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of MS000511 vs. ExPASy TrEMBL
Match: A0A0A0LAH3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 1.3e-151
Identity = 263/347 (75.79%), Postives = 300/347 (86.46%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MG+AVVY+   T FA FF+  PSN  +R+HQ+ TRRLG+K   PTFDPLV EMER A E 
Sbjct: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAE- 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
              RG NAI        +++ D+Y++YYDEGRL ISLRLLVLFPLLD+SPKDGVISYEEL
Sbjct: 61  --ERGENAIGVDNQN--HKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           SDWI  QA++RLNYRT K+L+F+DKNGD AISF EYLPQFTEEDIA+  T +GEAGWW++
Sbjct: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRK 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD D NGLL F+ELKDFLHPEDSSNYRIQNWLL +KMKRMD+D+DGKLNFDEFLHH
Sbjct: 181 QFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            +DIYK+YIEFETQG+DVP+AEEKFDELDLDEDE+LS EELRPLFQYLHPGE+SYAQHYT
Sbjct: 241 TYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYT 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           SHLI EADDNKDGYL++DEMLNHEYVFYS+VYE +NGDYEDDYHDEL
Sbjct: 301 SHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 342

BLAST of MS000511 vs. ExPASy TrEMBL
Match: A0A5A7U5L5 (Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001680 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.9e-150
Identity = 263/348 (75.57%), Postives = 300/348 (86.21%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MG+AVVY+   TAFA FF+  PSN   RN+Q+ TRRLG+K   PTFDPLV EMER   E 
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHSRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAE- 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
              RG NAI  +     +++ DAY++YYD+GRL ISLRLLVLFPLLD+SPKDGVISYEEL
Sbjct: 61  --ERGENAIGVENQN--HKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           SDWI  QA++RLNYRT K+L+F+DKNGD AISF EYLPQFT+EDIA+  T +GEAGWW +
Sbjct: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWWIK 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD D NGLL+F+ELKDFLHPEDSSNYRIQNWLL +KMKRMD+DRDGKLNFDEFLHH
Sbjct: 181 QFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            +DIYK+YIEFETQGDD+PTAEEKFDELDLDEDE+LSVEE+RPLFQYLHPGE+SYAQHYT
Sbjct: 241 TYDIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYT 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDD-YHDEL 348
           SHLI EADDNKDGYL++DEMLNHEY FYS+VYE +NGDYEDD YHDEL
Sbjct: 301 SHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of MS000511 vs. ExPASy TrEMBL
Match: A0A5D3CJD2 (Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001480 PE=4 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.9e-150
Identity = 263/348 (75.57%), Postives = 301/348 (86.49%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60
           MG+AVVY+   TAFA FF+  PSN  +RN+Q+ TRRLG+K   PTFDPLV EMER   E 
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHNRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAE- 60

Query: 61  GSSRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120
              RG NAI  +     +++ DAY++YYDEGRL ISLRLLVLFPLLD+SPKDGVISYEEL
Sbjct: 61  --ERGENAIGMENQN--HKITDAYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120

Query: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180
           SDWI  QA++RLNYRT K+L+F+DKNGD AISF EYLPQFT+EDIA+  T +GEAGWW +
Sbjct: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNDTGYGEAGWWIK 180

Query: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240
           QFTNAD D NGLL+F+ELKDFLHPEDSSNYRIQNWLL +KMKRMD+DRDGKLNFDEFLHH
Sbjct: 181 QFTNADVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHH 240

Query: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300
            ++IYK+YIEFETQGDD+PTAEEKFDELDLDEDE+LSVEE+RPLFQYLHPGE+SYAQHYT
Sbjct: 241 TYNIYKNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYT 300

Query: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDD-YHDEL 348
           SHLI EADDNKDGYL++DEMLNHEY FYS+VYE +NGDYEDD YHDEL
Sbjct: 301 SHLINEADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

BLAST of MS000511 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.0e-80
Identity = 156/353 (44.19%), Postives = 220/353 (62.32%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQE--LTRRLGYKLRAPTFDPLVEEMERYAE 60
           M + VVY  L     F  +            E  +TRR+G +L  P FDPLV  +ER + 
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQNQTQSIEGLITRRIGRRLEMPVFDPLVTRIERLSH 60

Query: 61  EHGSSRGRNAISGQRTTTA--NQVPDAYKDYY-DEGRLKISLRLLVLFPLLDHSPKDGVI 120
           E          +G +T  A   +  D +++Y+  E RL  ++R+  LFPLLD SP+DG +
Sbjct: 61  EKE--------AGTKTVEAAKEEKDDMFEEYFAQERRLNTTMRIKFLFPLLDASPRDGFV 120

Query: 121 SYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEA 180
           S +EL  W+++Q    + YRT KEL+  DK+ DG I+F EYLPQF+++DI K    HGEA
Sbjct: 121 SLKELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEA 180

Query: 181 GWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFD 240
           GWW  QF N+D D NG L+ EE  +FLHPEDS N   Q W+L+E+M  MD + DGKL + 
Sbjct: 181 GWWMEQFKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYK 240

Query: 241 EFLHHVFDIYKSYIEFETQGDD-VPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELS 300
           EF+ + +++YK + +FE + D+ VPT +  F E+D D+D  L  +ELRP+ QYL PGE+S
Sbjct: 241 EFVKNAYEMYKEFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRPILQYLQPGEMS 300

Query: 301 YAQHYTSHLIYEADDNKDGYLSLDEMLNHEYVFYSSVYEGRNGDYEDDYHDEL 348
           YA+ Y++ L +EAD++KDG LSL+EML+HE VFY +V+     D +   HDEL
Sbjct: 301 YAKFYSTFLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHEDLDDEDYFDHDEL 345

BLAST of MS000511 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 201.8 bits (512), Expect = 8.8e-52
Identity = 127/390 (32.56%), Postives = 201/390 (51.54%), Query Frame = 0

Query: 1   MGRAVVYLFLITAFAFFFV--FYPSNSPDRNHQELTRRLGYKLR-------APT------ 60
           M +A V L++       F+  + P    D +H        ++L+        PT      
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNFKPTRHDPVP 60

Query: 61  FDPLVEEMERYAEEH-------GSSRGRNAISGQRTTTA---------------NQVPDA 120
           FDPLV +MER  E+          S        Q+ TT                 +  DA
Sbjct: 61  FDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFMDA 120

Query: 121 YKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEELSDWIIEQAVQRLNYRTRKELDFH 180
                DE +  ++ RL++LFP +D SP DG ++  EL++W ++ + + + +RT+++LD H
Sbjct: 121 EDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLDVH 180

Query: 181 DKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKRQFTNA-DTDPNGLLNFEELKDFL 240
           D+N DG ISF EY P             + + GWWK +  NA D + +GLLN  E  DFL
Sbjct: 181 DRNKDGFISFSEYEPPSWVRKSDNNSFGY-DMGWWKEEHFNASDANGDGLLNLTEFNDFL 240

Query: 241 HPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHHVFDIYKSYIEFETQG----DDV 300
           HP D+ N ++  WL +E+++  D D+DGK++F+EF H +FD  ++Y E          D+
Sbjct: 241 HPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYHDL 300

Query: 301 P--TAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYTSHLIYEADDNKDGYLS 347
           P   A++ F +LD ++D  LS  EL P+   +HP E  YA+    ++I +AD +KD  L+
Sbjct: 301 PEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRRLT 360

BLAST of MS000511 vs. TAIR 10
Match: AT1G74740.1 (calcium-dependent protein kinase 30 )

HSP 1 Score: 48.5 bits (114), Expect = 1.2e-05
Identity = 39/142 (27.46%), Postives = 67/142 (47.18%), Query Frame = 0

Query: 102 LFPLLDHSPKDGVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLP--- 161
           +F L+D    DG ISY EL   + +   Q      +  ++  D NG+G + + E++    
Sbjct: 368 MFTLMD-DDNDGKISYLELRAGLRKVGSQLGEPEIKLLMEVADVNGNGCLDYGEFVAVII 427

Query: 162 --QFTEEDIAKRGTAHGEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWL 221
             Q  E D             +++ F   D D +G +  EEL++ L  E        N +
Sbjct: 428 HLQKMEND-----------EHFRQAFMFFDKDGSGYIESEELREALTDELGEP---DNSV 487

Query: 222 LREKMKRMDYDRDGKLNFDEFL 239
           + + M+ +D D+DGK+N+DEF+
Sbjct: 488 IIDIMREVDTDKDGKINYDEFV 494

BLAST of MS000511 vs. TAIR 10
Match: AT5G12180.1 (calcium-dependent protein kinase 17 )

HSP 1 Score: 48.5 bits (114), Expect = 1.2e-05
Identity = 32/126 (25.40%), Postives = 60/126 (47.62%), Query Frame = 0

Query: 113 GVISYEELSDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAH 172
           G I+ EEL   + +Q  +   Y  ++ ++  D +G+G I + E++      +   R    
Sbjct: 392 GTITLEELRQGLAKQGTRLSEYEVQQLMEAADADGNGTIDYGEFIAATMHINRLDREEHL 451

Query: 173 GEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKL 232
             A      F + D D +G +  EEL+  L     ++ R     ++E +  +D D DG++
Sbjct: 452 YSA------FQHFDKDNSGYITMEELEQALREFGMNDGRD----IKEIISEVDGDNDGRI 507

Query: 233 NFDEFL 239
           N+DEF+
Sbjct: 512 NYDEFV 507

BLAST of MS000511 vs. TAIR 10
Match: AT4G23650.1 (calcium-dependent protein kinase 6 )

HSP 1 Score: 47.4 bits (111), Expect = 2.8e-05
Identity = 59/226 (26.11%), Postives = 103/226 (45.58%), Query Frame = 0

Query: 105 LLDHSPKDGVISYEELS-DWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEE 164
           +L + PKD + + E L+  WI            R++ +  DK  D A+  R  + QF   
Sbjct: 316 MLKYDPKDRLTAAEVLNHPWI------------REDGEASDKPLDNAVLSR--MKQFRAM 375

Query: 165 DIAKR--------GTAHGEAGWWKRQFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNW 224
           +  K+          +  E    K  F + DTD NG++  EEL+  L P+  S  +I   
Sbjct: 376 NKLKKMALKVIAENLSEEEIIGLKEMFKSLDTDNNGIVTLEELRTGL-PKLGS--KISEA 435

Query: 225 LLREKMKRMDYDRDGKLNFDEFLHHVFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEL 284
            +R+ M+  D D DG +++ EF+     ++ + IE E   D + TA + FD    D    
Sbjct: 436 EIRQLMEAADMDGDGSIDYLEFISAT--MHMNRIERE---DHLYTAFQFFDN---DNSGY 495

Query: 285 LSVEELRPLFQYLHPGELSYAQHYTSHLIYEADDNKDGYLSLDEML 322
           +++EEL    +  + G+          +I E D ++DG ++ +E +
Sbjct: 496 ITMEELELAMKKYNMGD----DKSIKEIIAEVDTDRDGKINYEEFV 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141310.19.7e-20299.42reticulocalbin-2 [Momordica charantia][more]
XP_022945265.11.8e-15275.50reticulocalbin-2 isoform X1 [Cucurbita moschata][more]
XP_023541445.12.4e-15275.22reticulocalbin-2 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7013311.14.1e-15275.2245 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6574244.15.4e-15275.2245 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
B5X1863.2e-1427.46Calumenin-A OS=Salmo salar OX=8030 GN=calua PE=2 SV=1[more]
B5X4E04.2e-1427.20Calumenin-B OS=Salmo salar OX=8030 GN=calub PE=2 SV=1[more]
Q7ZUC21.6e-1327.0445 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1[more]
J3S9D94.6e-1327.76Reticulocalbin-2 OS=Crotalus adamanteus OX=8729 PE=1 SV=1[more]
O438526.0e-1327.71Calumenin OS=Homo sapiens OX=9606 GN=CALU PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CHQ24.7e-20299.42reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1[more]
A0A6J1G0G28.9e-15375.50reticulocalbin-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449565 PE=4 S... [more]
A0A0A0LAH31.3e-15175.79Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G238180 PE=4 SV=1[more]
A0A5A7U5L51.9e-15075.57Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001... [more]
A0A5D3CJD21.9e-15075.57Reticulocalbin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G001... [more]
Match NameE-valueIdentityDescription
AT4G27790.12.0e-8044.19Calcium-binding EF hand family protein [more]
AT5G08580.18.8e-5232.56Calcium-binding EF hand family protein [more]
AT1G74740.11.2e-0527.46calcium-dependent protein kinase 30 [more]
AT5G12180.11.2e-0525.40calcium-dependent protein kinase 17 [more]
AT4G23650.12.8e-0526.11calcium-dependent protein kinase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 216..244
e-value: 0.016
score: 24.4
coord: 135..163
e-value: 62.0
score: 3.3
coord: 177..205
e-value: 1.8
score: 15.7
coord: 260..288
e-value: 32.0
score: 5.6
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 301..320
e-value: 0.024
score: 14.4
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 144..155
e-value: 0.059
score: 12.9
coord: 221..237
e-value: 0.0089
score: 15.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 217..247
score: 9.045907
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 256..291
score: 9.352757
NoneNo IPR availableGENE3D1.10.238.10coord: 159..246
e-value: 7.3E-12
score: 47.5
NoneNo IPR availableGENE3D1.10.238.10coord: 247..325
e-value: 1.4E-9
score: 40.1
NoneNo IPR availableGENE3D1.10.238.10coord: 98..158
e-value: 9.4E-6
score: 27.7
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 1..347
NoneNo IPR availablePANTHERPTHR10827:SF77CALCIUM-BINDING EF HAND FAMILY PROTEINcoord: 1..347
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 308..320
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 144..156
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 186..198
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 225..237
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 174..337
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 98..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000511.1MS000511.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding