MS000504 (gene) Bitter gourd (TR) v1

Overview


Name	MS000504
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RWP-RK domain-containing protein
Location	scaffold44: 2134336 .. 2135108 (-)
RNA-Seq Expression	MS000504
Synteny	MS000504

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACTCCACAGAGCACTAGAGCTTCCTCCTCTGGATGTTGGGTTATTCGACTTCGACTTGGCCGACTCGCTTTCATTGCCATTACATTGGGAGAATCTTTCCCAGTTGGAGTTGGAGCTCCAAAGTCCGCCTGAATTTTTCTCGCCGGACAAACACATGACGGCCGACGAGAAGCCGCCGGCGAGGATGATAAAATTAGAATATGAGGAAGGGCAGCATGAACAACAACAACAACGTGAGCAAATTGAAAGAGGGTTTAATAGTAATTCAAGTTGGAGATCAGACGTTGATGAGAAGAAGAGGAGGAATAATATGAGATTGAGGAGATCAAAGAGCTCAGTGCTGGGTTTGGAAGAGATTCAAAACCACTTTCACATCCCAATATCAGAGGCAGCAAAACAGATGAATGTGGGTTTGACAGTGCTCAAAAAGAGGTGCAGAGAGCTCAATATCATGAGGTGGCCCCATAGAAAGCTCAAGAGCTTGAACTCTCTCATAAAAAATGTCAAGGTATATATATTGAGATCAAACAGCTAACCTTGTGTGTGTTCTTTTGATTTATGTATTTGGGTTGTCATTGTTTATTAGTGCAATTTGAAGTGCAGGAGATGGGGTTGTCAAGTGAGATTAAAGTGTTGGAGGAGCACAAGAGGCTCGTGGAAGAATTGCCAGATATGGACCTCACACACAGAACCAAGAGGCTCAGGCAAGCTTGTTTCAAGGCAAATTATAAGAAGAGAAGGTCCAATAATAATAGTTTTGCAACTCTTCAC

mRNA sequence

CCACTCCACAGAGCACTAGAGCTTCCTCCTCTGGATGTTGGGTTATTCGACTTCGACTTGGCCGACTCGCTTTCATTGCCATTACATTGGGAGAATCTTTCCCAGTTGGAGTTGGAGCTCCAAAGTCCGCCTGAATTTTTCTCGCCGGACAAACACATGACGGCCGACGAGAAGCCGCCGGCGAGGATGATAAAATTAGAATATGAGGAAGGGCAGCATGAACAACAACAACAACGTGAGCAAATTGAAAGAGGGTTTAATAGTAATTCAAGTTGGAGATCAGACGTTGATGAGAAGAAGAGGAGGAATAATATGAGATTGAGGAGATCAAAGAGCTCAGTGCTGGGTTTGGAAGAGATTCAAAACCACTTTCACATCCCAATATCAGAGGCAGCAAAACAGATGAATGTGGGTTTGACAGTGCTCAAAAAGAGGTGCAGAGAGCTCAATATCATGAGGTGGCCCCATAGAAAGCTCAAGAGCTTGAACTCTCTCATAAAAAATGTCAAGGAGATGGGGTTGTCAAGTGAGATTAAAGTGTTGGAGGAGCACAAGAGGCTCGTGGAAGAATTGCCAGATATGGACCTCACACACAGAACCAAGAGGCTCAGGCAAGCTTGTTTCAAGGCAAATTATAAGAAGAGAAGGTCCAATAATAATAGTTTTGCAACTCTTCAC

Coding sequence (CDS)

CCACTCCACAGAGCACTAGAGCTTCCTCCTCTGGATGTTGGGTTATTCGACTTCGACTTGGCCGACTCGCTTTCATTGCCATTACATTGGGAGAATCTTTCCCAGTTGGAGTTGGAGCTCCAAAGTCCGCCTGAATTTTTCTCGCCGGACAAACACATGACGGCCGACGAGAAGCCGCCGGCGAGGATGATAAAATTAGAATATGAGGAAGGGCAGCATGAACAACAACAACAACGTGAGCAAATTGAAAGAGGGTTTAATAGTAATTCAAGTTGGAGATCAGACGTTGATGAGAAGAAGAGGAGGAATAATATGAGATTGAGGAGATCAAAGAGCTCAGTGCTGGGTTTGGAAGAGATTCAAAACCACTTTCACATCCCAATATCAGAGGCAGCAAAACAGATGAATGTGGGTTTGACAGTGCTCAAAAAGAGGTGCAGAGAGCTCAATATCATGAGGTGGCCCCATAGAAAGCTCAAGAGCTTGAACTCTCTCATAAAAAATGTCAAGGAGATGGGGTTGTCAAGTGAGATTAAAGTGTTGGAGGAGCACAAGAGGCTCGTGGAAGAATTGCCAGATATGGACCTCACACACAGAACCAAGAGGCTCAGGCAAGCTTGTTTCAAGGCAAATTATAAGAAGAGAAGGTCCAATAATAATAGTTTTGCAACTCTTCAC

Protein sequence

PLHRALELPPLDVGLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSPDKHMTADEKPPARMIKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH

Homology

[Close]

BLAST of MS000504 vs. NCBI nr
Match: XP_022141143.1 (protein RKD4 [Momordica charantia])

HSP 1 Score: 427.6 bits (1098), Expect = 6.9e-116
Identity = 219/223 (98.21%), Postives = 221/223 (99.10%), Query Frame = 0


Query: 4   RALELPPLDVGLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSPDKHMTADEKPPARM 63
           +ALELPPLDVGLFDFDLADSLSLP HWENLSQLELELQSPPEFFSPDKHMTADEKPPARM
Sbjct: 29  KALELPPLDVGLFDFDLADSLSLPFHWENLSQLELELQSPPEFFSPDKHMTADEKPPARM 88

Query: 64  IKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLEEIQNH 123
           IKLEYEEGQHEQQQQREQIERGFNS+SSW SDVDEKKRRNNMRLRRSKSSVLGLEEIQNH
Sbjct: 89  IKLEYEEGQHEQQQQREQIERGFNSDSSWISDVDEKKRRNNMRLRRSKSSVLGLEEIQNH 148

Query: 124 FHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIKVLEE 183
           FHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIKVLEE
Sbjct: 149 FHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIKVLEE 208

Query: 184 HKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH 227
           HKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH
Sbjct: 209 HKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH 251

[Close]

BLAST of MS000504 vs. NCBI nr
Match: XP_023542025.1 (protein RKD2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 247.3 bits (630), Expect = 1.3e-61
Identity = 140/221 (63.35%), Postives = 168/221 (76.02%), Query Frame = 0


Query: 5   ALELPPLDV-GLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSP-----DKHMTADEK 64
           ALELPPLDV G FDF   +SLSLP HW+++S+LE E+ S  +   P     ++HM ADE 
Sbjct: 54  ALELPPLDVDGSFDF---NSLSLPFHWDDISELEAEILSLDKHMIPVTPLYEEHMVADET 113

Query: 65  PPARMIKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLE 124
            P RM+KL+ ++   E Q+    IE G +S      +  E++ R  MR  R  +SVLGLE
Sbjct: 114 KPERMVKLKDDDMSRELQEVTRMIETGLSSCKEEEEEEYERRVRLIMRNGRRTNSVLGLE 173

Query: 125 EIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEI 184
           EIQ HFHIPI+EAAK MNVGLTVLK+RCREL+IMRWPHRKLKSLNSLI NV+EMGL++EI
Sbjct: 174 EIQKHFHIPITEAAKAMNVGLTVLKRRCRELHIMRWPHRKLKSLNSLITNVQEMGLTNEI 233

Query: 185 KVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNN 220
           K LEEHKRLVEELPDMDLTHRT+RLRQACFKANYKK+R +N
Sbjct: 234 KGLEEHKRLVEELPDMDLTHRTRRLRQACFKANYKKKRRSN 271

[Close]

BLAST of MS000504 vs. NCBI nr
Match: XP_022968161.1 (protein RKD4 [Cucurbita maxima])

HSP 1 Score: 245.7 bits (626), Expect = 3.7e-61
Identity = 144/233 (61.80%), Postives = 173/233 (74.25%), Query Frame = 0


Query: 3   HRALELPPLDV-GLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSP------DKHMTA 62
           H ALELPPLDV G FDF   +SLSLP HW+++S+LE E+ S  +   P      +  +  
Sbjct: 27  HEALELPPLDVDGSFDF---NSLSLPFHWDDISELESEILSLDKHMIPVTPLYEEDMVAV 86

Query: 63  DEKPPARMIKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVD--EKKRRNNMRLRRSKSS 122
           DE  P RM+KL+ ++   E Q+    IE G    S+W+ + +  EK+ R  MR  R KSS
Sbjct: 87  DETKPERMVKLKDDDMSRELQEVTRMIETGL---SNWKEEEEECEKRVRLIMRNGRRKSS 146

Query: 123 VLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMG 182
           VLGLEEIQ HFHIPI+EAAK M+VGLTVLK+RCRELNIMRWPHRKLKSLNSLI NV+EMG
Sbjct: 147 VLGLEEIQKHFHIPITEAAKAMSVGLTVLKRRCRELNIMRWPHRKLKSLNSLITNVQEMG 206

Query: 183 LSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH 227
           L++EIK LEEHKRLVEELP+MDLTHRT+RLRQACFKANYKK+R +N   A LH
Sbjct: 207 LTNEIKGLEEHKRLVEELPNMDLTHRTRRLRQACFKANYKKKRRSN--VAVLH 251

[Close]

BLAST of MS000504 vs. NCBI nr
Match: KAG6574250.1 (Protein RKD4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 227.6 bits (579), Expect = 1.0e-55
Identity = 135/226 (59.73%), Postives = 165/226 (73.01%), Query Frame = 0


Query: 3   HRALELPPLDV-GLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSP-----DKHMTA- 62
           H ALELPPLDV   FDF   +SLSLP  W+++S+LE E+    +   P     D+ M A 
Sbjct: 27  HEALELPPLDVDASFDF---NSLSLPFPWDDISELEAEILWLDKHMIPVTPLNDEDMVAV 86

Query: 63  DEKPPARMIKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNN--MRLRRSKSS 122
           DE    RM+KL+  +G   + Q+  ++     S+   + + +E +RR    MR RR K S
Sbjct: 87  DETKAERMVKLKDNDGTSRELQEETRVIETELSSCKEKGEEEECERRMRLIMRNRRRKIS 146

Query: 123 VLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMG 182
            LGL+EIQ HFHIPI+EAAK MNVGLTVLK+RCREL+IMRWPHRKLKSLNSLI NV+EMG
Sbjct: 147 ALGLDEIQKHFHIPITEAAKAMNVGLTVLKRRCRELHIMRWPHRKLKSLNSLITNVQEMG 206

Query: 183 LSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNN 220
           L++EIK LEEHKRLVEELPDMDLTHRT+RLRQACFKANYKK+R +N
Sbjct: 207 LTNEIKGLEEHKRLVEELPDMDLTHRTRRLRQACFKANYKKKRRSN 249

[Close]

BLAST of MS000504 vs. NCBI nr
Match: XP_022945007.1 (protein RKD2 [Cucurbita moschata])

HSP 1 Score: 227.3 bits (578), Expect = 1.4e-55
Identity = 134/226 (59.29%), Postives = 163/226 (72.12%), Query Frame = 0


Query: 3   HRALELPPLDV-GLFDFDLADSLSLPLHWENLSQLELEL------QSPPEFFSPDKHMTA 62
           H ALELPPLDV   FDF   +SLSLP HW+++S+LE E+        P    + +  +  
Sbjct: 27  HEALELPPLDVDASFDF---NSLSLPFHWDDISELEAEILWLDKHMIPVTPLNDEDMLAV 86

Query: 63  DEKPPARMIKLEYEEG-QHEQQQQREQIERGFNS-NSSWRSDVDEKKRRNNMRLRRSKSS 122
           DE    RM+KL+  +G   E Q++   IE G +S   +   +  E++ R  MR  R KS 
Sbjct: 87  DETKAERMVKLKDNDGTSRELQEETRVIETGLSSCKENEEEEECERRMRLIMRNGRRKSK 146

Query: 123 VLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMG 182
           VLGL+EIQ HF IPI+EAAK MNVGLTVLK+RCREL+IMRWPHRKLKSLNSLI NV+EMG
Sbjct: 147 VLGLDEIQKHFDIPITEAAKAMNVGLTVLKRRCRELHIMRWPHRKLKSLNSLITNVQEMG 206

Query: 183 LSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNN 220
           L++EIK LEEHKRLVEELPD DLTHRT+RLRQACFKANYKK+R +N
Sbjct: 207 LTNEIKGLEEHKRLVEELPDTDLTHRTRRLRQACFKANYKKKRRSN 249

[Close]

BLAST of MS000504 vs. ExPASy Swiss-Prot
Match: Q9LVU8 (Protein RKD4 OS=Arabidopsis thaliana OX=3702 GN=RKD4 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.6e-33
Identity = 74/127 (58.27%), Postives = 97/127 (76.38%), Query Frame = 0


Query: 96  VDEKKRRNNMRLRRSKSSVLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWP 155
           V  KK+RN   L++ +   L + EI+  F  PI +AAK++NVGLTVLKKRCREL I RWP
Sbjct: 132 VTVKKKRN---LKKKRQDKLEMSEIKQFFDRPIMKAAKELNVGLTVLKKRCRELGIYRWP 191

Query: 156 HRKLKSLNSLIKNVKEMGLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKR 215
           HRKLKSLNSLIKN+K +G+  E+K LEEH+ L+E+ PD +L+  TK+LRQACFKANYK+R
Sbjct: 192 HRKLKSLNSLIKNLKNVGMEEEVKNLEEHRFLIEQEPDAELSDGTKKLRQACFKANYKRR 251

Query: 216 RSNNNSF 223
           +S  + +
Sbjct: 252 KSLGDDY 255

[Close]

BLAST of MS000504 vs. ExPASy Swiss-Prot
Match: Q9FGD1 (Protein RKD3 OS=Arabidopsis thaliana OX=3702 GN=RKD3 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-25
Identity = 57/115 (49.57%), Postives = 87/115 (75.65%), Query Frame = 0


Query: 118 EEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEM----- 177
           E ++ +F++PI++AAK++N+G+T+LKKRCREL I RWPHRKL SLN+LI N+K++     
Sbjct: 162 EMMKQYFYMPITKAAKELNIGVTLLKKRCRELGIPRWPHRKLTSLNALIANLKDLLGNTK 221

Query: 178 ------GLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNS 222
                  L + +++LE  K+++EE+PD++   +TKRLRQACFKA YK+RR  ++S
Sbjct: 222 GRTPKSKLRNALELLEMEKKMIEEVPDLEFGDKTKRLRQACFKAKYKRRRLFSSS 276

[Close]

BLAST of MS000504 vs. ExPASy Swiss-Prot
Match: Q9CA66 (Protein RKD2 OS=Arabidopsis thaliana OX=3702 GN=RKD2 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.8e-25
Identity = 66/171 (38.60%), Postives = 107/171 (62.57%), Query Frame = 0


Query: 67  EYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRL--------RRSKSSVLGLE 126
           E E+ + +     ++ E G    ++ +  ++E+ R +++R+          S  + L  E
Sbjct: 79  ETEQNRGDGASSEKKRENGTVKETTKKRKINERHREHSVRIISDITTYTTSSAPTTLSKE 138

Query: 127 EIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEM------ 186
            +  +F++PI++AA  +NVGLT+LK+RCREL I RWPHRKL SLN+LI NVKE+      
Sbjct: 139 TVSRYFYMPITQAAIALNVGLTLLKRRCRELGIRRWPHRKLMSLNTLISNVKELQKMEGE 198

Query: 187 ----GLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNN 220
                L   +++LE+ KR +E+LPD++   +TKRLRQACFKAN+K+++  +
Sbjct: 199 ENAEKLQDALEMLEKEKRTIEDLPDLEFKDKTKRLRQACFKANHKRKKKRS 249

[Close]

BLAST of MS000504 vs. ExPASy Swiss-Prot
Match: Q9M9U9 (Protein RKD1 OS=Arabidopsis thaliana OX=3702 GN=RKD1 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 7.6e-25
Identity = 66/147 (44.90%), Postives = 92/147 (62.59%), Query Frame = 0


Query: 94  SDVDEKKRRNNMRLRR---------SKSSVLGLEEIQNHFHIPISEAAKQMNVGLTVLKK 153
           SD  +K      + RR         S S  L  E I  +F++PI++AA+++N+GLT+LKK
Sbjct: 98  SDFAKKSETTTTKKRRCREECFSSCSVSKTLSKETISLYFYMPITQAARELNIGLTLLKK 157

Query: 154 RCRELNIMRWPHRKLKSLNSLIKNVKEM----------GLSSEIKVLEEHKRLVEELPDM 213
           RCREL I RWPHRKL SL  LI NVKE+           L + ++ LE+ K+ +E+LPD+
Sbjct: 158 RCRELGIKRWPHRKLMSLQKLISNVKELEKMEGEENEDKLRNALEKLEKEKKTIEKLPDL 217

Query: 214 DLTHRTKRLRQACFKANYKKRRSNNNS 222
               +TKRLRQACFKAN+K++R +  S
Sbjct: 218 KFEDKTKRLRQACFKANHKRKRRSGMS 244

[Close]

BLAST of MS000504 vs. ExPASy Swiss-Prot
Match: O81791 (Protein RKD5 OS=Arabidopsis thaliana OX=3702 GN=RKD5 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.5e-14
Identity = 57/168 (33.93%), Postives = 94/168 (55.95%), Query Frame = 0


Query: 67  EYEEGQHEQQQQRE-QIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLEEIQNHFH 126
           E EE  +E+ +  E + ++   S S  ++++ +KK+R   R      + L LEE+  +F 
Sbjct: 197 ESEESVNEKTEHSEFENDKTEQSESDAKTEILKKKKRTPSR----HVAELSLEELSKYFD 256

Query: 127 IPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKE-------------M 186
           + I EA++ + VGLTVLKK+CRE  I RWPHRK+KSL+ LI +++              M
Sbjct: 257 LTIVEASRNLKVGLTVLKKKCREFGIPRWPHRKIKSLDCLIHDLQREAEKQQEKNEAAAM 316

Query: 187 GLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNN 221
            ++ + + LE  KR + + P M++   TK+ RQ  FK  ++  R+  N
Sbjct: 317 AVAKKQEKLETEKRNIVKRPFMEIGIETKKFRQENFKKRHRASRAKKN 360

[Close]

BLAST of MS000504 vs. ExPASy TrEMBL
Match: A0A6J1CJM6 (protein RKD4 OS=Momordica charantia OX=3673 GN=LOC111011607 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.3e-116
Identity = 219/223 (98.21%), Postives = 221/223 (99.10%), Query Frame = 0


Query: 4   RALELPPLDVGLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSPDKHMTADEKPPARM 63
           +ALELPPLDVGLFDFDLADSLSLP HWENLSQLELELQSPPEFFSPDKHMTADEKPPARM
Sbjct: 29  KALELPPLDVGLFDFDLADSLSLPFHWENLSQLELELQSPPEFFSPDKHMTADEKPPARM 88

Query: 64  IKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLEEIQNH 123
           IKLEYEEGQHEQQQQREQIERGFNS+SSW SDVDEKKRRNNMRLRRSKSSVLGLEEIQNH
Sbjct: 89  IKLEYEEGQHEQQQQREQIERGFNSDSSWISDVDEKKRRNNMRLRRSKSSVLGLEEIQNH 148

Query: 124 FHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIKVLEE 183
           FHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIKVLEE
Sbjct: 149 FHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIKVLEE 208

Query: 184 HKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH 227
           HKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH
Sbjct: 209 HKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH 251

[Close]

BLAST of MS000504 vs. ExPASy TrEMBL
Match: A0A6J1HU37 (protein RKD4 OS=Cucurbita maxima OX=3661 GN=LOC111467479 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.8e-61
Identity = 144/233 (61.80%), Postives = 173/233 (74.25%), Query Frame = 0


Query: 3   HRALELPPLDV-GLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSP------DKHMTA 62
           H ALELPPLDV G FDF   +SLSLP HW+++S+LE E+ S  +   P      +  +  
Sbjct: 27  HEALELPPLDVDGSFDF---NSLSLPFHWDDISELESEILSLDKHMIPVTPLYEEDMVAV 86

Query: 63  DEKPPARMIKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVD--EKKRRNNMRLRRSKSS 122
           DE  P RM+KL+ ++   E Q+    IE G    S+W+ + +  EK+ R  MR  R KSS
Sbjct: 87  DETKPERMVKLKDDDMSRELQEVTRMIETGL---SNWKEEEEECEKRVRLIMRNGRRKSS 146

Query: 123 VLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMG 182
           VLGLEEIQ HFHIPI+EAAK M+VGLTVLK+RCRELNIMRWPHRKLKSLNSLI NV+EMG
Sbjct: 147 VLGLEEIQKHFHIPITEAAKAMSVGLTVLKRRCRELNIMRWPHRKLKSLNSLITNVQEMG 206

Query: 183 LSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNSFATLH 227
           L++EIK LEEHKRLVEELP+MDLTHRT+RLRQACFKANYKK+R +N   A LH
Sbjct: 207 LTNEIKGLEEHKRLVEELPNMDLTHRTRRLRQACFKANYKKKRRSN--VAVLH 251

[Close]

BLAST of MS000504 vs. ExPASy TrEMBL
Match: A0A6J1FZR5 (protein RKD2 OS=Cucurbita moschata OX=3662 GN=LOC111449372 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 6.6e-56
Identity = 134/226 (59.29%), Postives = 163/226 (72.12%), Query Frame = 0


Query: 3   HRALELPPLDV-GLFDFDLADSLSLPLHWENLSQLELEL------QSPPEFFSPDKHMTA 62
           H ALELPPLDV   FDF   +SLSLP HW+++S+LE E+        P    + +  +  
Sbjct: 27  HEALELPPLDVDASFDF---NSLSLPFHWDDISELEAEILWLDKHMIPVTPLNDEDMLAV 86

Query: 63  DEKPPARMIKLEYEEG-QHEQQQQREQIERGFNS-NSSWRSDVDEKKRRNNMRLRRSKSS 122
           DE    RM+KL+  +G   E Q++   IE G +S   +   +  E++ R  MR  R KS 
Sbjct: 87  DETKAERMVKLKDNDGTSRELQEETRVIETGLSSCKENEEEEECERRMRLIMRNGRRKSK 146

Query: 123 VLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMG 182
           VLGL+EIQ HF IPI+EAAK MNVGLTVLK+RCREL+IMRWPHRKLKSLNSLI NV+EMG
Sbjct: 147 VLGLDEIQKHFDIPITEAAKAMNVGLTVLKRRCRELHIMRWPHRKLKSLNSLITNVQEMG 206

Query: 183 LSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNN 220
           L++EIK LEEHKRLVEELPD DLTHRT+RLRQACFKANYKK+R +N
Sbjct: 207 LTNEIKGLEEHKRLVEELPDTDLTHRTRRLRQACFKANYKKKRRSN 249

[Close]

BLAST of MS000504 vs. ExPASy TrEMBL
Match: A0A5D3E104 (Protein RKD4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G003820 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 8.1e-46
Identity = 121/228 (53.07%), Postives = 148/228 (64.91%), Query Frame = 0


Query: 1   PLHRAL-ELPPLDVGLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSPDKHMTADEKP 60
           PLH  L ELPPLD      D+     LP H ++   LE +     EF S +  M   E+ 
Sbjct: 32  PLHNELDELPPLDNDEL-LDVNSRSLLPFHLDDNDVLEFD----SEFLSRELEMVEIERN 91

Query: 61  PARMIKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLEE 120
                  E EE + E+ ++R ++    N N                  RR  S VLGLEE
Sbjct: 92  GFSSTWKEEEEEEEEEGEKRLRLMGNNNGNK-----------------RRKGSFVLGLEE 151

Query: 121 IQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIK 180
           I+ +FHIPISEAAK+MNVGLTVLK+RCR+LNIMRWPHRK KSLNSLI+NVKEMGL++E+K
Sbjct: 152 IRKYFHIPISEAAKEMNVGLTVLKRRCRQLNIMRWPHRKHKSLNSLIQNVKEMGLTNEVK 211

Query: 181 VLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKK-RRSNNNSFATLH 227
            LEEHKRL+EE+P+MDLTH  KRLRQACFKANYKK RR  +N+ A+LH
Sbjct: 212 GLEEHKRLLEEMPNMDLTHTVKRLRQACFKANYKKNRRLRSNNIASLH 237

[Close]

BLAST of MS000504 vs. ExPASy TrEMBL
Match: E5GBA6 (Transcription factor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 5.3e-45
Identity = 120/228 (52.63%), Postives = 147/228 (64.47%), Query Frame = 0


Query: 1   PLHRAL-ELPPLDVGLFDFDLADSLSLPLHWENLSQLELELQSPPEFFSPDKHMTADEKP 60
           PLH  L ELPPLD      D+     LP H ++   LE +     EF S +  M   E+ 
Sbjct: 26  PLHNELDELPPLDNDEL-LDVNSRSLLPFHLDDNDVLEFD----SEFLSRELEMVEIERN 85

Query: 61  PARMIKLEYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLEE 120
                  E EE + E++ ++     G                 NN   RR  S VLGLEE
Sbjct: 86  GFSSTWKEEEEEEEEEEGEKRLRLMG----------------NNNGNKRRKGSFVLGLEE 145

Query: 121 IQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEMGLSSEIK 180
           I+ +FHIPIS+AAK+MNVGLTVLK+RCR+LNIMRWPHRK KSLNSLI+NVKEMGL++E+K
Sbjct: 146 IRKYFHIPISKAAKEMNVGLTVLKRRCRQLNIMRWPHRKHKSLNSLIQNVKEMGLTNEVK 205

Query: 181 VLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKK-RRSNNNSFATLH 227
            LEEHKRL+EE+P+MDLTH  KRLRQACFKANYKK RR  +N+ A+LH
Sbjct: 206 GLEEHKRLLEEMPNMDLTHTVKRLRQACFKANYKKNRRLRSNNIASLH 232

[Close]

BLAST of MS000504 vs. TAIR 10
Match: AT5G53040.1 (RWP-RK domain-containing protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.9e-34
Identity = 74/127 (58.27%), Postives = 97/127 (76.38%), Query Frame = 0


Query: 96  VDEKKRRNNMRLRRSKSSVLGLEEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWP 155
           V  KK+RN   L++ +   L + EI+  F  PI +AAK++NVGLTVLKKRCREL I RWP
Sbjct: 132 VTVKKKRN---LKKKRQDKLEMSEIKQFFDRPIMKAAKELNVGLTVLKKRCRELGIYRWP 191

Query: 156 HRKLKSLNSLIKNVKEMGLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKR 215
           HRKLKSLNSLIKN+K +G+  E+K LEEH+ L+E+ PD +L+  TK+LRQACFKANYK+R
Sbjct: 192 HRKLKSLNSLIKNLKNVGMEEEVKNLEEHRFLIEQEPDAELSDGTKKLRQACFKANYKRR 251

Query: 216 RSNNNSF 223
           +S  + +
Sbjct: 252 KSLGDDY 255

[Close]

BLAST of MS000504 vs. TAIR 10
Match: AT5G66990.1 (RWP-RK domain-containing protein )

HSP 1 Score: 116.3 bits (290), Expect = 3.2e-26
Identity = 57/115 (49.57%), Postives = 87/115 (75.65%), Query Frame = 0


Query: 118 EEIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEM----- 177
           E ++ +F++PI++AAK++N+G+T+LKKRCREL I RWPHRKL SLN+LI N+K++     
Sbjct: 162 EMMKQYFYMPITKAAKELNIGVTLLKKRCRELGIPRWPHRKLTSLNALIANLKDLLGNTK 221

Query: 178 ------GLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNNS 222
                  L + +++LE  K+++EE+PD++   +TKRLRQACFKA YK+RR  ++S
Sbjct: 222 GRTPKSKLRNALELLEMEKKMIEEVPDLEFGDKTKRLRQACFKAKYKRRRLFSSS 276

[Close]

BLAST of MS000504 vs. TAIR 10
Match: AT1G74480.1 (RWP-RK domain-containing protein )

HSP 1 Score: 115.9 bits (289), Expect = 4.1e-26
Identity = 66/171 (38.60%), Postives = 107/171 (62.57%), Query Frame = 0


Query: 67  EYEEGQHEQQQQREQIERGFNSNSSWRSDVDEKKRRNNMRL--------RRSKSSVLGLE 126
           E E+ + +     ++ E G    ++ +  ++E+ R +++R+          S  + L  E
Sbjct: 79  ETEQNRGDGASSEKKRENGTVKETTKKRKINERHREHSVRIISDITTYTTSSAPTTLSKE 138

Query: 127 EIQNHFHIPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKEM------ 186
            +  +F++PI++AA  +NVGLT+LK+RCREL I RWPHRKL SLN+LI NVKE+      
Sbjct: 139 TVSRYFYMPITQAAIALNVGLTLLKRRCRELGIRRWPHRKLMSLNTLISNVKELQKMEGE 198

Query: 187 ----GLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNN 220
                L   +++LE+ KR +E+LPD++   +TKRLRQACFKAN+K+++  +
Sbjct: 199 ENAEKLQDALEMLEKEKRTIEDLPDLEFKDKTKRLRQACFKANHKRKKKRS 249

[Close]

BLAST of MS000504 vs. TAIR 10
Match: AT1G18790.1 (RWP-RK domain-containing protein )

HSP 1 Score: 115.5 bits (288), Expect = 5.4e-26
Identity = 66/147 (44.90%), Postives = 92/147 (62.59%), Query Frame = 0


Query: 94  SDVDEKKRRNNMRLRR---------SKSSVLGLEEIQNHFHIPISEAAKQMNVGLTVLKK 153
           SD  +K      + RR         S S  L  E I  +F++PI++AA+++N+GLT+LKK
Sbjct: 98  SDFAKKSETTTTKKRRCREECFSSCSVSKTLSKETISLYFYMPITQAARELNIGLTLLKK 157

Query: 154 RCRELNIMRWPHRKLKSLNSLIKNVKEM----------GLSSEIKVLEEHKRLVEELPDM 213
           RCREL I RWPHRKL SL  LI NVKE+           L + ++ LE+ K+ +E+LPD+
Sbjct: 158 RCRELGIKRWPHRKLMSLQKLISNVKELEKMEGEENEDKLRNALEKLEKEKKTIEKLPDL 217

Query: 214 DLTHRTKRLRQACFKANYKKRRSNNNS 222
               +TKRLRQACFKAN+K++R +  S
Sbjct: 218 KFEDKTKRLRQACFKANHKRKRRSGMS 244

[Close]

BLAST of MS000504 vs. TAIR 10
Match: AT4G35590.1 (RWP-RK domain-containing protein )

HSP 1 Score: 80.1 bits (196), Expect = 2.5e-15
Identity = 57/168 (33.93%), Postives = 94/168 (55.95%), Query Frame = 0


Query: 67  EYEEGQHEQQQQRE-QIERGFNSNSSWRSDVDEKKRRNNMRLRRSKSSVLGLEEIQNHFH 126
           E EE  +E+ +  E + ++   S S  ++++ +KK+R   R      + L LEE+  +F 
Sbjct: 197 ESEESVNEKTEHSEFENDKTEQSESDAKTEILKKKKRTPSR----HVAELSLEELSKYFD 256

Query: 127 IPISEAAKQMNVGLTVLKKRCRELNIMRWPHRKLKSLNSLIKNVKE-------------M 186
           + I EA++ + VGLTVLKK+CRE  I RWPHRK+KSL+ LI +++              M
Sbjct: 257 LTIVEASRNLKVGLTVLKKKCREFGIPRWPHRKIKSLDCLIHDLQREAEKQQEKNEAAAM 316

Query: 187 GLSSEIKVLEEHKRLVEELPDMDLTHRTKRLRQACFKANYKKRRSNNN 221
            ++ + + LE  KR + + P M++   TK+ RQ  FK  ++  R+  N
Sbjct: 317 AVAKKQEKLETEKRNIVKRPFMEIGIETKKFRQENFKKRHRASRAKKN 360

The following BLAST results are available for this feature:

BLAST of MS000504 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141143.1	6.9e-116	98.21	protein RKD4 [Momordica charantia]	[more]
XP_023542025.1	1.3e-61	63.35	protein RKD2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022968161.1	3.7e-61	61.80	protein RKD4 [Cucurbita maxima]	[more]
KAG6574250.1	1.0e-55	59.73	Protein RKD4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022945007.1	1.4e-55	59.29	protein RKD2 [Cucurbita moschata]	[more]

BLAST of MS000504 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVU8	2.6e-33	58.27	Protein RKD4 OS=Arabidopsis thaliana OX=3702 GN=RKD4 PE=3 SV=1	[more]
Q9FGD1	4.5e-25	49.57	Protein RKD3 OS=Arabidopsis thaliana OX=3702 GN=RKD3 PE=3 SV=1	[more]
Q9CA66	5.8e-25	38.60	Protein RKD2 OS=Arabidopsis thaliana OX=3702 GN=RKD2 PE=2 SV=1	[more]
Q9M9U9	7.6e-25	44.90	Protein RKD1 OS=Arabidopsis thaliana OX=3702 GN=RKD1 PE=3 SV=1	[more]
O81791	3.5e-14	33.93	Protein RKD5 OS=Arabidopsis thaliana OX=3702 GN=RKD5 PE=3 SV=1	[more]

BLAST of MS000504 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJM6	3.3e-116	98.21	protein RKD4 OS=Momordica charantia OX=3673 GN=LOC111011607 PE=4 SV=1	[more]
A0A6J1HU37	1.8e-61	61.80	protein RKD4 OS=Cucurbita maxima OX=3661 GN=LOC111467479 PE=4 SV=1	[more]
A0A6J1FZR5	6.6e-56	59.29	protein RKD2 OS=Cucurbita moschata OX=3662 GN=LOC111449372 PE=4 SV=1	[more]
A0A5D3E104	8.1e-46	53.07	Protein RKD4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G003820 ...	[more]
E5GBA6	5.3e-45	52.63	Transcription factor OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]

BLAST of MS000504 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53040.1	1.9e-34	58.27	RWP-RK domain-containing protein	[more]
AT5G66990.1	3.2e-26	49.57	RWP-RK domain-containing protein	[more]
AT1G74480.1	4.1e-26	38.60	RWP-RK domain-containing protein	[more]
AT1G18790.1	5.4e-26	44.90	RWP-RK domain-containing protein	[more]
AT4G35590.1	2.5e-15	33.93	RWP-RK domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003035	RWP-RK domain	PFAM	PF02042	RWP-RK	coord: 116..162 e-value: 6.3E-23 score: 80.5
IPR003035	RWP-RK domain	PROSITE	PS51519	RWP_RK	coord: 102..183 score: 17.324911
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..100
None	No IPR available	PANTHER	PTHR46373:SF20	PROTEIN RKD1-RELATED	coord: 69..218
IPR044607	Transcription factor RKD-like	PANTHER	PTHR46373	PROTEIN RKD4	coord: 69..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000504.1	MS000504.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003700	DNA-binding transcription factor activity