MS000503 (gene) Bitter gourd (TR) v1

BLAST of MS000503 vs. NCBI nr
Match: XP_023552510.1 (uncharacterized protein At4g00950-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 333.2 bits (853), Expect = 1.6e-87
Identity = 172/218 (78.90%), Postives = 187/218 (85.78%), Query Frame = 0

Query: 1   MRPATGENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE 60
           MR ATGENPSSTPPKLSLFSLPRQPPEPPG++TPPLHASISVPFQWEEAPGKPRPFGIIE
Sbjct: 1   MRLATGENPSSTPPKLSLFSLPRQPPEPPGLVTPPLHASISVPFQWEEAPGKPRPFGIIE 60

Query: 61  -SSKPKSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETR 120
            +SKPKSARSLDLPPRLF D KVAHF+SPTTA DDP+VG  LSS+LSFRFPD+WAET TR
Sbjct: 61  PNSKPKSARSLDLPPRLFTDTKVAHFSSPTTAVDDPVVGQDLSSNLSFRFPDTWAETATR 120

Query: 121 EGKDGKYVGSRRWISFRKNKEIPK-------AAGGGDGGGRDDRRGDGGTRVKITRFRSR 180
           EGK GKYVGSRRW+SFRKNKE+PK       +AGG + GGR+   G+G TRVKITRFRSR
Sbjct: 121 EGKHGKYVGSRRWMSFRKNKEVPKGASEIPRSAGGDNSGGRNAGSGEGETRVKITRFRSR 180

Query: 181 RSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
           R LF  S +KS  IASIYGSLKQVIPWRRK D+ R VS
Sbjct: 181 RRLFRKSDTKSQLIASIYGSLKQVIPWRRKPDETRTVS 218

BLAST of MS000503 vs. NCBI nr
Match: XP_022923037.1 (uncharacterized protein At4g00950-like [Cucurbita moschata])

HSP 1 Score: 332.0 bits (850), Expect = 3.7e-87
Identity = 172/218 (78.90%), Postives = 186/218 (85.32%), Query Frame = 0

Query: 1   MRPATGENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE 60
           MR A GENPSSTPPKLSLFSLPRQPPEPPG++TPPLHASISVPFQWEEAPGKPRPFG+IE
Sbjct: 1   MRLAKGENPSSTPPKLSLFSLPRQPPEPPGLVTPPLHASISVPFQWEEAPGKPRPFGMIE 60

Query: 61  -SSKPKSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETR 120
            +SKP+SARSLDLPPRLF D KVAHF+SPTTA DDP+VG  LSS+LSFRFPD+WAET TR
Sbjct: 61  PNSKPRSARSLDLPPRLFTDTKVAHFSSPTTAVDDPVVGQDLSSNLSFRFPDTWAETATR 120

Query: 121 EGKDGKYVGSRRWISFRKNKEIPK-------AAGGGDGGGRDDRRGDGGTRVKITRFRSR 180
           EGK GKYVGSRRW+SFRKNKE+PK       +AGG   GGR+   GDG TRVKITRFRSR
Sbjct: 121 EGKHGKYVGSRRWMSFRKNKEVPKGASEIPRSAGGDKSGGRNAGSGDGETRVKITRFRSR 180

Query: 181 RSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
           RSLF  S SKS  IASIYGSLKQVIPWRRK D+ R VS
Sbjct: 181 RSLFRKSDSKSQLIASIYGSLKQVIPWRRKPDETRGVS 218

BLAST of MS000503 vs. NCBI nr
Match: KAG6576648.1 (Protein ABIL2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 327.4 bits (838), Expect = 9.0e-86
Identity = 168/210 (80.00%), Postives = 181/210 (86.19%), Query Frame = 0

Query: 6   GENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE-SSKP 65
           GENPSSTPPKLSLFSLPRQPPEPPG++TPPLHASISVPFQWEEAPGKPRPFGIIE +SKP
Sbjct: 761 GENPSSTPPKLSLFSLPRQPPEPPGLVTPPLHASISVPFQWEEAPGKPRPFGIIEPNSKP 820

Query: 66  KSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETREGKDG 125
           +SARSLDLPPRLF D KVAHF+SPTTA DDP+VG  LSS+LSFRFPD+WAET TREGK G
Sbjct: 821 RSARSLDLPPRLFTDTKVAHFSSPTTAVDDPVVGQDLSSNLSFRFPDTWAETATREGKHG 880

Query: 126 KYVGSRRWISFRKNKEIPK-------AAGGGDGGGRDDRRGDGGTRVKITRFRSRRSLFG 185
           KYVGSRRW+SFRKNKE+PK       +AGG   GGR+   GDG TRVKITRFRSRRSLF 
Sbjct: 881 KYVGSRRWMSFRKNKEVPKGASEIPRSAGGDKSGGRNAGSGDGETRVKITRFRSRRSLFR 940

Query: 186 TSTSKSHFIASIYGSLKQVIPWRRKQDDMR 208
            S SKS  IASIYGSLKQVIPWRRK D+ R
Sbjct: 941 KSDSKSQLIASIYGSLKQVIPWRRKPDETR 970

BLAST of MS000503 vs. NCBI nr
Match: KAG7014699.1 (Protein ABIL2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327.4 bits (838), Expect = 9.0e-86
Identity = 168/210 (80.00%), Postives = 181/210 (86.19%), Query Frame = 0

Query: 6    GENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE-SSKP 65
            GENPSSTPPKLSLFSLPRQPPEPPG++TPPLHASISVPFQWEEAPGKPRPFGIIE +SKP
Sbjct: 805  GENPSSTPPKLSLFSLPRQPPEPPGLVTPPLHASISVPFQWEEAPGKPRPFGIIEPNSKP 864

Query: 66   KSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETREGKDG 125
            +SARSLDLPPRLF D KVAHF+SPTTA DDP+VG  LSS+LSFRFPD+WAET TREGK G
Sbjct: 865  RSARSLDLPPRLFTDTKVAHFSSPTTAVDDPVVGQDLSSNLSFRFPDTWAETATREGKHG 924

Query: 126  KYVGSRRWISFRKNKEIPK-------AAGGGDGGGRDDRRGDGGTRVKITRFRSRRSLFG 185
            KYVGSRRW+SFRKNKE+PK       +AGG   GGR+   GDG TRVKITRFRSRRSLF 
Sbjct: 925  KYVGSRRWMSFRKNKEVPKGASEIPRSAGGDKSGGRNAGSGDGETRVKITRFRSRRSLFR 984

Query: 186  TSTSKSHFIASIYGSLKQVIPWRRKQDDMR 208
             S SKS  IASIYGSLKQVIPWRRK D+ R
Sbjct: 985  KSDSKSQLIASIYGSLKQVIPWRRKPDETR 1014

BLAST of MS000503 vs. NCBI nr
Match: XP_022984515.1 (uncharacterized protein At4g00950-like [Cucurbita maxima])

HSP 1 Score: 326.2 bits (835), Expect = 2.0e-85
Identity = 169/218 (77.52%), Postives = 184/218 (84.40%), Query Frame = 0

Query: 1   MRPATGENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE 60
           MR  TGENPSSTPPKLSLFSLPRQPPEPPG++TPPLHASISVPFQWEEAPGKPRPFGIIE
Sbjct: 1   MRLETGENPSSTPPKLSLFSLPRQPPEPPGLVTPPLHASISVPFQWEEAPGKPRPFGIIE 60

Query: 61  -SSKPKSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETR 120
            +SKP+SARSLDLPPRLF D KVAHF+SPTTA DDP+V   LSS+LSFRFPD+WAET TR
Sbjct: 61  PNSKPRSARSLDLPPRLFTDTKVAHFSSPTTAVDDPVVAQDLSSNLSFRFPDTWAETATR 120

Query: 121 EGKDGKYVGSRRWISFRKNKEIPK-------AAGGGDGGGRDDRRGDGGTRVKITRFRSR 180
           EGK GKYVGSRRW+SFRKNKE+PK       +AGG + GGR+   G+G TRVKITRFRSR
Sbjct: 121 EGKHGKYVGSRRWMSFRKNKEVPKGASEIPRSAGGDNSGGRNVGSGEGETRVKITRFRSR 180

Query: 181 RSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
           RS    S SKS  IASIYGSLKQVIPWRRK D+ R VS
Sbjct: 181 RSFLRKSDSKSQLIASIYGSLKQVIPWRRKPDETRGVS 218

BLAST of MS000503 vs. ExPASy Swiss-Prot
Match: Q9M160 (Uncharacterized protein At4g00950 OS=Arabidopsis thaliana OX=3702 GN=At4g00950 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.3e-05
Identity = 74/269 (27.51%), Postives = 100/269 (37.17%), Query Frame = 0

Query: 21  LPRQPPEPPGMLTPPLHASI--SVPFQWEEAPGKPRPFGIIESSKPKSA----------- 80
           LP +P      ++ P+H+SI  SVPF WEE PGKP+      SS   S+           
Sbjct: 21  LPTKPNTHSHSMSSPIHSSISASVPFSWEEEPGKPKQHSTSSSSSSSSSPLTSYSSSPFE 80

Query: 81  --RSLDLPPRLFADAK----VAHFASPTTAGDDP--LVGPHLSSSLSFRFPDSWAET--- 140
             +SL+LPPRL    K    V    SP T  D P  +       S SFR     +     
Sbjct: 81  THKSLELPPRLHLLEKDGGSVTKLHSPITVFDGPYSMTTSKRMDSPSFRMMVKGSADCYG 140

Query: 141 ETREGKDGKY----VGSRR-------------------WISFRKNKEIPKAAGGGDGG-- 200
             R   DG      VGS++                   +  FR+ + +      G G   
Sbjct: 141 SFRSDIDGDLEDLEVGSKQQENLSSGSLAVVKKRGRLGFFGFRRRRALKGKTEFGRGSYV 200

Query: 201 -------------------------GRDDRRGDGGTR------VKITRFRSRRSLFGT-- 204
                                    G DD  G   ++      VKI+   SR   F T  
Sbjct: 201 FPSSVDRESEYSRKEEEEEKEDKRFGYDDTDGISCSQSSRFCDVKISSI-SRTGSFSTLP 260

BLAST of MS000503 vs. ExPASy TrEMBL
Match: A0A6J1E520 (uncharacterized protein At4g00950-like OS=Cucurbita moschata OX=3662 GN=LOC111430836 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.8e-87
Identity = 172/218 (78.90%), Postives = 186/218 (85.32%), Query Frame = 0

Query: 1   MRPATGENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE 60
           MR A GENPSSTPPKLSLFSLPRQPPEPPG++TPPLHASISVPFQWEEAPGKPRPFG+IE
Sbjct: 1   MRLAKGENPSSTPPKLSLFSLPRQPPEPPGLVTPPLHASISVPFQWEEAPGKPRPFGMIE 60

Query: 61  -SSKPKSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETR 120
            +SKP+SARSLDLPPRLF D KVAHF+SPTTA DDP+VG  LSS+LSFRFPD+WAET TR
Sbjct: 61  PNSKPRSARSLDLPPRLFTDTKVAHFSSPTTAVDDPVVGQDLSSNLSFRFPDTWAETATR 120

Query: 121 EGKDGKYVGSRRWISFRKNKEIPK-------AAGGGDGGGRDDRRGDGGTRVKITRFRSR 180
           EGK GKYVGSRRW+SFRKNKE+PK       +AGG   GGR+   GDG TRVKITRFRSR
Sbjct: 121 EGKHGKYVGSRRWMSFRKNKEVPKGASEIPRSAGGDKSGGRNAGSGDGETRVKITRFRSR 180

Query: 181 RSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
           RSLF  S SKS  IASIYGSLKQVIPWRRK D+ R VS
Sbjct: 181 RSLFRKSDSKSQLIASIYGSLKQVIPWRRKPDETRGVS 218

BLAST of MS000503 vs. ExPASy TrEMBL
Match: A0A6J1J8U1 (uncharacterized protein At4g00950-like OS=Cucurbita maxima OX=3661 GN=LOC111482786 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 9.7e-86
Identity = 169/218 (77.52%), Postives = 184/218 (84.40%), Query Frame = 0

Query: 1   MRPATGENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE 60
           MR  TGENPSSTPPKLSLFSLPRQPPEPPG++TPPLHASISVPFQWEEAPGKPRPFGIIE
Sbjct: 1   MRLETGENPSSTPPKLSLFSLPRQPPEPPGLVTPPLHASISVPFQWEEAPGKPRPFGIIE 60

Query: 61  -SSKPKSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETR 120
            +SKP+SARSLDLPPRLF D KVAHF+SPTTA DDP+V   LSS+LSFRFPD+WAET TR
Sbjct: 61  PNSKPRSARSLDLPPRLFTDTKVAHFSSPTTAVDDPVVAQDLSSNLSFRFPDTWAETATR 120

Query: 121 EGKDGKYVGSRRWISFRKNKEIPK-------AAGGGDGGGRDDRRGDGGTRVKITRFRSR 180
           EGK GKYVGSRRW+SFRKNKE+PK       +AGG + GGR+   G+G TRVKITRFRSR
Sbjct: 121 EGKHGKYVGSRRWMSFRKNKEVPKGASEIPRSAGGDNSGGRNVGSGEGETRVKITRFRSR 180

Query: 181 RSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
           RS    S SKS  IASIYGSLKQVIPWRRK D+ R VS
Sbjct: 181 RSFLRKSDSKSQLIASIYGSLKQVIPWRRKPDETRGVS 218

BLAST of MS000503 vs. ExPASy TrEMBL
Match: E5GBA5 (Uncharacterized protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.4e-84
Identity = 173/227 (76.21%), Postives = 184/227 (81.06%), Query Frame = 0

Query: 1   MRPATGENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE 60
           MR  TGENPSSTPPKLSLFSLPRQPPEPPGM+TPPLHASISVPFQWEEAPGKPRPFGIIE
Sbjct: 1   MRFVTGENPSSTPPKLSLFSLPRQPPEPPGMVTPPLHASISVPFQWEEAPGKPRPFGIIE 60

Query: 61  -SSKPKSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAE---- 120
            +SKPKSARSLDLPPRLFADAKVAHFASPTTA D+P+ G  LSSSLSFRFPD+WAE    
Sbjct: 61  PNSKPKSARSLDLPPRLFADAKVAHFASPTTAVDEPIFGRDLSSSLSFRFPDTWAETVRA 120

Query: 121 ------TETREGKDGKYVGSRRWISFRKNKEIPKA------AGGGDGGGRDDRRGDGGTR 180
                 T TREGKDGKYVGSRRW+SFRKNKEIPK+       GGGD   R+    DG TR
Sbjct: 121 TATATATGTREGKDGKYVGSRRWMSFRKNKEIPKSGSEIAVTGGGD---RNVGSSDGETR 180

Query: 181 VKITRFRSRRSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
           VKITRFRSRRS F    SKSHFIA+IYGSLKQ I WRRK D+M  +S
Sbjct: 181 VKITRFRSRRSFFRKPNSKSHFIANIYGSLKQAISWRRKGDEMENIS 224

BLAST of MS000503 vs. ExPASy TrEMBL
Match: A0A1S3CS64 (uncharacterized protein LOC103504144 OS=Cucumis melo OX=3656 GN=LOC103504144 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.4e-84
Identity = 173/227 (76.21%), Postives = 184/227 (81.06%), Query Frame = 0

Query: 1   MRPATGENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE 60
           MR  TGENPSSTPPKLSLFSLPRQPPEPPGM+TPPLHASISVPFQWEEAPGKPRPFGIIE
Sbjct: 1   MRFVTGENPSSTPPKLSLFSLPRQPPEPPGMVTPPLHASISVPFQWEEAPGKPRPFGIIE 60

Query: 61  -SSKPKSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAE---- 120
            +SKPKSARSLDLPPRLFADAKVAHFASPTTA D+P+ G  LSSSLSFRFPD+WAE    
Sbjct: 61  PNSKPKSARSLDLPPRLFADAKVAHFASPTTAVDEPIFGRDLSSSLSFRFPDTWAETVRA 120

Query: 121 ------TETREGKDGKYVGSRRWISFRKNKEIPKA------AGGGDGGGRDDRRGDGGTR 180
                 T TREGKDGKYVGSRRW+SFRKNKEIPK+       GGGD   R+    DG TR
Sbjct: 121 TATATATGTREGKDGKYVGSRRWMSFRKNKEIPKSGSEIAVTGGGD---RNVGSSDGETR 180

Query: 181 VKITRFRSRRSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
           VKITRFRSRRS F    SKSHFIA+IYGSLKQ I WRRK D+M  +S
Sbjct: 181 VKITRFRSRRSFFRKPNSKSHFIANIYGSLKQAISWRRKGDEMENIS 224

BLAST of MS000503 vs. ExPASy TrEMBL
Match: A0A5A7U5K0 (Myosin heavy chain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G001580 PE=4 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.2e-83
Identity = 170/220 (77.27%), Postives = 181/220 (82.27%), Query Frame = 0

Query: 6    GENPSSTPPKLSLFSLPRQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIE-SSKP 65
            GENPSSTPPKLSLFSLPRQPPEPPGM+TPPLHASISVPFQWEEAPGKPRPFGIIE +SKP
Sbjct: 811  GENPSSTPPKLSLFSLPRQPPEPPGMVTPPLHASISVPFQWEEAPGKPRPFGIIEPNSKP 870

Query: 66   KSARSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAE--------T 125
            KSARSLDLPPRLFADAKVAHFASPTTA D+P+ G  LSSSLSFRFPD+WAE        T
Sbjct: 871  KSARSLDLPPRLFADAKVAHFASPTTAVDEPIFGRDLSSSLSFRFPDTWAETVRATATAT 930

Query: 126  ETREGKDGKYVGSRRWISFRKNKEIPKA------AGGGDGGGRDDRRGDGGTRVKITRFR 185
             TREGKDGKYVGSRRW+SFRKNKEIPK+       GGGD   R+    DG TRVKITRFR
Sbjct: 931  GTREGKDGKYVGSRRWMSFRKNKEIPKSGSEIAVTGGGD---RNVGSSDGETRVKITRFR 990

Query: 186  SRRSLFGTSTSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
            SRRS F    SKSHFIA+IYGSLKQ I WRRK D+M  +S
Sbjct: 991  SRRSFFRKPNSKSHFIANIYGSLKQAISWRRKGDEMENIS 1027

BLAST of MS000503 vs. TAIR 10
Match: AT4G27810.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G53030.1); Has 73 Blast hits to 66 proteins in 11 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 73; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 105.1 bits (261), Expect = 6.8e-23
Identity = 79/211 (37.44%), Postives = 108/211 (51.18%), Query Frame = 0

Query: 14  PKLSLFSLP-RQPPEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIESSKPKSA----- 73
           PKL LFS+P  +  + PG+ TPP++ + SVPF WEEAPGKPR   + + +KP ++     
Sbjct: 15  PKLPLFSIPFNRACDTPGLATPPVNIAGSVPFLWEEAPGKPR---VSDENKPLASKQNER 74

Query: 74  --------RSLDLPPRLFADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETR 133
                   R L+LPPRLF  A      SPTT  D P   P  S S+  R         + 
Sbjct: 75  EGGGGGVVRCLELPPRLFFPAD--DEPSPTTVLDGPYDVPRRSLSVIRR---------SE 134

Query: 134 EGKDGKYVGSRRWISFRKNKEIPKAAGGGDGGGRDDRRGDGGTRVKITRFRSRRSLFGTS 193
              +G++  SR   S        +   GG           GGT VKI+R R + SL   S
Sbjct: 135 RASEGRFEFSRSTNS--------RCCDGG-----------GGTTVKISRVRRKGSLLNLS 192

Query: 194 TSKSHFIASIYGSLKQVIPWRRKQDDMRKVS 211
            SKS F+A +Y   KQVIPWRR+Q+++ ++S
Sbjct: 195 HSKSQFLARVYQGFKQVIPWRRRQENLPRMS 192

BLAST of MS000503 vs. TAIR 10
Match: AT5G53030.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27810.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 87.0 bits (214), Expect = 1.9e-17
Identity = 85/235 (36.17%), Postives = 111/235 (47.23%), Query Frame = 0

Query: 10  SSTPPKLSLFSLPRQP---PEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIESSKPKS 69
           SST  +L LFS P         PG+ TPP++ + SVPF WEEAPGKPR          K 
Sbjct: 13  SSTRKQLPLFSYPMNNIAYETTPGLATPPVNIAGSVPFLWEEAPGKPRRVKKPARLNQKG 72

Query: 70  -ARSLDLPPRLF--ADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETREG-- 129
             RSL+LPPRL    ++   +  SPTT  D    GP+     S   P S A      G  
Sbjct: 73  VVRSLELPPRLVLPGESTTVNEPSPTTVLD----GPYDLRRRSLSLPRSAAVIRKLRGVP 132

Query: 130 -----KDGKYV-GSRRWISFRKNKEIPKAA--------GGGD-------GGGRDDRRGDG 189
                K+ + V GS RW SF   KE+ +           G D       GGG  +  GD 
Sbjct: 133 APAPEKEERLVGGSSRWGSFGNCKEVSEGIFDFSRFRDDGYDCRRDWAGGGGVGNFAGD- 192

Query: 190 GTRVKITRFRSRRSLFGTS-TSKSHF----IASIYGSLKQVIPWRRKQDDMRKVS 211
             +VK+ R   + S F  S T+KS F     A +Y   KQVIPW+RKQ+++ + +
Sbjct: 193 -AKVKLYRIIKKGSFFNLSHTTKSDFWLKMQARVYEGFKQVIPWKRKQENLERTN 241

BLAST of MS000503 vs. TAIR 10
Match: AT5G53030.2 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G27810.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-12
Identity = 76/211 (36.02%), Postives = 95/211 (45.02%), Query Frame = 0

Query: 10  SSTPPKLSLFSLPRQP---PEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIESSKPKS 69
           SST  +L LFS P         PG+ TPP++ + SVPF WEEAPGKPR          K 
Sbjct: 13  SSTRKQLPLFSYPMNNIAYETTPGLATPPVNIAGSVPFLWEEAPGKPRRVKKPARLNQKG 72

Query: 70  -ARSLDLPPRLF--ADAKVAHFASPTTAGDDPLVGPHLSSSLSFRFPDSWAETETREG-- 129
             RSL+LPPRL    ++   +  SPTT  D    GP+     S   P S A      G  
Sbjct: 73  VVRSLELPPRLVLPGESTTVNEPSPTTVLD----GPYDLRRRSLSLPRSAAVIRKLRGVP 132

Query: 130 -----KDGKYV-GSRRWISFRKNKEIPKAA--------GGGD-------GGGRDDRRGDG 189
                K+ + V GS RW SF   KE+ +           G D       GGG  +  GD 
Sbjct: 133 APAPEKEERLVGGSSRWGSFGNCKEVSEGIFDFSRFRDDGYDCRRDWAGGGGVGNFAGD- 192

Query: 190 GTRVKITRFRSRRSLFGTS-TSKSHFIASIY 191
             +VK+ R   + S F  S T+KS F  S Y
Sbjct: 193 -AKVKLYRIIKKGSFFNLSHTTKSDFWVSFY 217

BLAST of MS000503 vs. TAIR 10
Match: AT4G00950.1 (Protein of unknown function (DUF688) )

HSP 1 Score: 51.6 bits (122), Expect = 8.9e-07
Identity = 74/269 (27.51%), Postives = 100/269 (37.17%), Query Frame = 0

Query: 21  LPRQPPEPPGMLTPPLHASI--SVPFQWEEAPGKPRPFGIIESSKPKSA----------- 80
           LP +P      ++ P+H+SI  SVPF WEE PGKP+      SS   S+           
Sbjct: 21  LPTKPNTHSHSMSSPIHSSISASVPFSWEEEPGKPKQHSTSSSSSSSSSPLTSYSSSPFE 80

Query: 81  --RSLDLPPRLFADAK----VAHFASPTTAGDDP--LVGPHLSSSLSFRFPDSWAET--- 140
             +SL+LPPRL    K    V    SP T  D P  +       S SFR     +     
Sbjct: 81  THKSLELPPRLHLLEKDGGSVTKLHSPITVFDGPYSMTTSKRMDSPSFRMMVKGSADCYG 140

Query: 141 ETREGKDGKY----VGSRR-------------------WISFRKNKEIPKAAGGGDGG-- 200
             R   DG      VGS++                   +  FR+ + +      G G   
Sbjct: 141 SFRSDIDGDLEDLEVGSKQQENLSSGSLAVVKKRGRLGFFGFRRRRALKGKTEFGRGSYV 200

Query: 201 -------------------------GRDDRRGDGGTR------VKITRFRSRRSLFGT-- 204
                                    G DD  G   ++      VKI+   SR   F T  
Sbjct: 201 FPSSVDRESEYSRKEEEEEKEDKRFGYDDTDGISCSQSSRFCDVKISSI-SRTGSFSTLP 260

BLAST of MS000503 vs. TAIR 10
Match: AT2G46535.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF688 (InterPro:IPR007789); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF688) (TAIR:AT3G61840.1); Has 48 Blast hits to 48 proteins in 8 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 48; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 42.4 bits (98), Expect = 5.4e-04
Identity = 24/64 (37.50%), Postives = 31/64 (48.44%), Query Frame = 0

Query: 26 PEPPGMLTPPLHASISVPFQWEEAPGKPRPFGIIESSKPKS-ARSLDLPPRLFADAKVAH 85
          P  P +   P+H   SVPF WE+ PGKP+        +P S  + LDLPPRL    +   
Sbjct: 29 PASPRVFASPIHTLASVPFCWEDQPGKPK-----HPLRPLSYPKCLDLPPRLLLPGEFTQ 87

Query: 86 FASP 89
             P
Sbjct: 89 MPLP 87