MS000486 (gene) Bitter gourd (TR) v1

BLAST of MS000486 vs. NCBI nr
Match: XP_022140831.1 (glutamate synthase [NADH], amyloplastic isoform X1 [Momordica charantia])

HSP 1 Score: 4434.4 bits (11500), Expect = 0.0e+00
Identity = 2219/2228 (99.60%), Postives = 2221/2228 (99.69%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF
Sbjct: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKE      AAKD
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKE------AAKD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT
Sbjct: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGW+SKVLDITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWQSKVLDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH
Sbjct: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS
Sbjct: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL
Sbjct: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740
            LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP
Sbjct: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740

Query: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800
            LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG
Sbjct: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800

Query: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860
            RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA
Sbjct: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860

Query: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920
            GLAAADQLNKMGH VTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN
Sbjct: 1861 GLAAADQLNKMGHAVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920

Query: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980
            ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS
Sbjct: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980

Query: 1981 NLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040
            NLE+GNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ
Sbjct: 1981 NLEDGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040

Query: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100
            WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF
Sbjct: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100

Query: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160
            KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG
Sbjct: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160

Query: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTS 2220
            DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTS
Sbjct: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTS 2220

Query: 2221 SSRHTVMT 2229
            SSRHTVMT
Sbjct: 2221 SSRHTVMT 2222

BLAST of MS000486 vs. NCBI nr
Match: XP_023552487.1 (glutamate synthase [NADH], amyloplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 4262.6 bits (11054), Expect = 0.0e+00
Identity = 2114/2229 (94.84%), Postives = 2177/2229 (97.67%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSLLKL AAPS+FDK+SVKP LNV  KAR+G RAARCSASK A+RSLNVSEKKFF
Sbjct: 1    MLAKSGSLLKLTAAPSSFDKSSVKPQLNVNSKARIGARAARCSASKAASRSLNVSEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSGR+QFWH DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGRVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAG+L+ALPH++FKE      AA+D
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGLLLALPHDFFKE------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESK+VF KVAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKKVFAKVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            L TEPVIEQVFLTPSTRSKVDLEKQMYILRRLS++AI+AALNLEHGGARDFYICSLSSRT
Sbjct: 241  LQTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSVIAIQAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKP+QLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPIQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMD QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDAQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK+QKIELKD+VGS+DKSE+T P IAGALPTS D+DNME MGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKRQKIELKDVVGSIDKSELTTPSIAGALPTSADDDNMETMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLPFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPL+SIE+MEAIK+MNYRGWRSKV+DITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLVSIEEMEAIKQMNYRGWRSKVVDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAI+EGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIREGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIPAKS+GE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPAKSNGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVE ASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGTSIPLDEVEAASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM  LPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMESLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMS+LG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSKLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKSLPVYIE+PINNVNRAVGTMLSHEVTKRYHMAGLP ETIHIKF GS
Sbjct: 1381 DQKLIALSKSALEKSLPVYIESPINNVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFNGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGI LELEGDSNDYVGKGLSGGKIVVYPP+ SLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRRSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDI+ LKMMIQQHQRHTSSNLAKEVL+NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDIVALKMMIQQHQRHTSSNLAKEVLDNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKV PREYKRVLANMKAQ A KE SEPAAK+ EEESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVIPREYKRVLANMKAQGATKEVSEPAAKDVEEESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740
            LNG+SKQVE+TEPPKRPTE PDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP
Sbjct: 1681 LNGDSKQVEKTEPPKRPTETPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740

Query: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800
            LLKTQSARCMDCGTPFCHQE+SGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG
Sbjct: 1741 LLKTQSARCMDCGTPFCHQESSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800

Query: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860
            RVCPAPCEGSCVLGIIENPVSIK+IECAIIDKAFEEGWM PRPPLTRSGKRVAIVGSGPA
Sbjct: 1801 RVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMTPRPPLTRSGKRVAIVGSGPA 1860

Query: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920
            GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN
Sbjct: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920

Query: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980
            AN+GTDPSY+LDRL+DENDALVLAVGATKPRDLPVPGREL+GVHFAMEFLHSNTKSLLDS
Sbjct: 1921 ANVGTDPSYSLDRLQDENDALVLAVGATKPRDLPVPGRELSGVHFAMEFLHSNTKSLLDS 1980

Query: 1981 NLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040
            NL++G YISAKDKKVVVIGGGDTGTDCIGTSIRHGCS+VVNLELLPQPPQTRAPGNPWPQ
Sbjct: 1981 NLQDGKYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSVVNLELLPQPPQTRAPGNPWPQ 2040

Query: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100
            WPRIFRVDYGHQEAA KFGKDPRSYEVLTKRFIGDENG VKGLE++RVQWEKDANGRFQF
Sbjct: 2041 WPRIFRVDYGHQEAATKFGKDPRSYEVLTKRFIGDENGVVKGLEVIRVQWEKDANGRFQF 2100

Query: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160
            KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFST+VDGIFAAG
Sbjct: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTSVDGIFAAG 2160

Query: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQD-ST 2220
            DCRRGQSLVVWAI+EGRQAAAQVDKY SKEEKN+LVGEGGYEGVG  SQDYNNRQQD S+
Sbjct: 2161 DCRRGQSLVVWAISEGRQAAAQVDKYLSKEEKNLLVGEGGYEGVGKSSQDYNNRQQDSSS 2220

Query: 2221 SSSRHTVMT 2229
            SSSRHT+MT
Sbjct: 2221 SSSRHTIMT 2223

BLAST of MS000486 vs. NCBI nr
Match: XP_022922726.1 (glutamate synthase [NADH], amyloplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 4257.2 bits (11040), Expect = 0.0e+00
Identity = 2112/2230 (94.71%), Postives = 2176/2230 (97.58%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSL+KL AAPS+FDK+SVKP LNV  KAR+G RAARCSASK A+RSLNV+EKKFF
Sbjct: 1    MLAKSGSLIKLTAAPSSFDKSSVKPQLNVNSKARIGARAARCSASKAASRSLNVTEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSG +QFWH DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGGVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAG+L+ALPHE+FKE      AA+D
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGLLLALPHEFFKE------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESK+VF KVAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKKVFAKVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            L TEPVIEQVFLTPSTRSKVDLEKQMYILRRLS+VAI+AALNLEHGGARDFYICSLSSRT
Sbjct: 241  LQTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSVVAIQAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKP+QLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPIQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMD QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDAQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK+QKIELKD+VGS+DKSE+T P IAGALPTS D+DNME MGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKRQKIELKDVVGSIDKSELTTPSIAGALPTSADDDNMETMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLPFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPL+SIE+MEAIK+MNYRGWRSKV+DITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLVSIEEMEAIKQMNYRGWRSKVVDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAI+EGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIREGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIPAKS+GE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPAKSNGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVE ASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGTSIPLDEVEAASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM  LPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMESLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMS+LG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSKLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKSLPVYIE+PINNVNRAVGTMLSHEVTKRYHMAGLP ETIHIKF GS
Sbjct: 1381 DQKLIALSKSALEKSLPVYIESPINNVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFNGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGI LELEGDSNDYVGKGLSGGKIVVYPP+ SLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRRSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDI+ LKMMIQQHQRHTSSNLAKEVL+NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDIVALKMMIQQHQRHTSSNLAKEVLDNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKRVLANMKAQ A KE SEPAAK+ EEESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRVLANMKAQGATKEVSEPAAKDVEEESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740
            LNG+SKQVE+TEPPKRPTE  DAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP
Sbjct: 1681 LNGDSKQVEKTEPPKRPTETLDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740

Query: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800
            LLKTQSARCMDCGTPFCHQE+SGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG
Sbjct: 1741 LLKTQSARCMDCGTPFCHQESSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800

Query: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860
            RVCPAPCEGSCVLGIIENPVSIK+IECAIIDKAFEEGWM PRPPLTRSGKRVAI+GSGPA
Sbjct: 1801 RVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMTPRPPLTRSGKRVAIIGSGPA 1860

Query: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920
            GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN
Sbjct: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920

Query: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980
            AN+GTDPSY+LDRL+DENDALVLAVGATKPRDLPVPGREL+GVHFAMEFLHSNTKSLLDS
Sbjct: 1921 ANVGTDPSYSLDRLQDENDALVLAVGATKPRDLPVPGRELSGVHFAMEFLHSNTKSLLDS 1980

Query: 1981 NLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040
            NL++G YISAKDKKVVVIGGGDTGTDCIGTSIRHGCS+VVNLELLPQPPQTRAPGNPWPQ
Sbjct: 1981 NLQDGKYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSVVNLELLPQPPQTRAPGNPWPQ 2040

Query: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100
            WPRIFRVDYGHQEAA KFGKDPRSYEVLTKRFIGDENG VKGLE++RVQWEKDANGRFQF
Sbjct: 2041 WPRIFRVDYGHQEAATKFGKDPRSYEVLTKRFIGDENGVVKGLEVIRVQWEKDANGRFQF 2100

Query: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160
            KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFST+VDGIFAAG
Sbjct: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTSVDGIFAAG 2160

Query: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQD--S 2220
            DCRRGQSLVVWAI+EGRQAAAQVDKY SKEEKN+LVGEGGYEGVG  SQDYNNRQQD  S
Sbjct: 2161 DCRRGQSLVVWAISEGRQAAAQVDKYLSKEEKNLLVGEGGYEGVGKSSQDYNNRQQDSSS 2220

Query: 2221 TSSSRHTVMT 2229
            +SSSRHT+MT
Sbjct: 2221 SSSSRHTIMT 2224

BLAST of MS000486 vs. NCBI nr
Match: XP_023552486.1 (glutamate synthase [NADH], amyloplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 4256.8 bits (11039), Expect = 0.0e+00
Identity = 2114/2233 (94.67%), Postives = 2177/2233 (97.49%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSLLKL AAPS+FDK+SVKP LNV  KAR+G RAARCSASK A+RSLNVSEKKFF
Sbjct: 1    MLAKSGSLLKLTAAPSSFDKSSVKPQLNVNSKARIGARAARCSASKAASRSLNVSEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSGR+QFWH DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGRVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAG+L+ALPH++FKE      AA+D
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGLLLALPHDFFKE------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESK+VF KVAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKKVFAKVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            L TEPVIEQVFLTPSTRSKVDLEKQMYILRRLS++AI+AALNLEHGGARDFYICSLSSRT
Sbjct: 241  LQTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSVIAIQAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKP+QLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPIQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMD QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDAQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK+QKIELKD+VGS+DKSE+T P IAGALPTS D+DNME MGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKRQKIELKDVVGSIDKSELTTPSIAGALPTSADDDNMETMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLPFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPL+SIE+MEAIK+MNYRGWRSKV+DITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLVSIEEMEAIKQMNYRGWRSKVVDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAI+EGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIREGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIPAKS+GE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPAKSNGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVE ASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGTSIPLDEVEAASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM  LPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMESLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMS+LG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSKLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKSLPVYIE+PINNVNRAVGTMLSHEVTKRYHMAGLP ETIHIKF GS
Sbjct: 1381 DQKLIALSKSALEKSLPVYIESPINNVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFNGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGI LELEGDSNDYVGKGLSGGKIVVYPP+ SLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRRSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDI+ LKMMIQQHQRHTSSNLAKEVL+NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDIVALKMMIQQHQRHTSSNLAKEVLDNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKV PREYKRVLANMKAQ A KE SEPAAK+ EEESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVIPREYKRVLANMKAQGATKEVSEPAAKDVEEESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQ----VEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES 1740
            LNG+SKQ    VE+TEPPKRPTE PDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES
Sbjct: 1681 LNGDSKQLDPKVEKTEPPKRPTETPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES 1740

Query: 1741 KPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFP 1800
            KPGPLLKTQSARCMDCGTPFCHQE+SGCPLGNKIPEFNELVYQNRWREALERLLETNNFP
Sbjct: 1741 KPGPLLKTQSARCMDCGTPFCHQESSGCPLGNKIPEFNELVYQNRWREALERLLETNNFP 1800

Query: 1801 EFTGRVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVG 1860
            EFTGRVCPAPCEGSCVLGIIENPVSIK+IECAIIDKAFEEGWM PRPPLTRSGKRVAIVG
Sbjct: 1801 EFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMTPRPPLTRSGKRVAIVG 1860

Query: 1861 SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN 1920
            SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN
Sbjct: 1861 SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN 1920

Query: 1921 FVVNANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKS 1980
            FVVNAN+GTDPSY+LDRL+DENDALVLAVGATKPRDLPVPGREL+GVHFAMEFLHSNTKS
Sbjct: 1921 FVVNANVGTDPSYSLDRLQDENDALVLAVGATKPRDLPVPGRELSGVHFAMEFLHSNTKS 1980

Query: 1981 LLDSNLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGN 2040
            LLDSNL++G YISAKDKKVVVIGGGDTGTDCIGTSIRHGCS+VVNLELLPQPPQTRAPGN
Sbjct: 1981 LLDSNLQDGKYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSVVNLELLPQPPQTRAPGN 2040

Query: 2041 PWPQWPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANG 2100
            PWPQWPRIFRVDYGHQEAA KFGKDPRSYEVLTKRFIGDENG VKGLE++RVQWEKDANG
Sbjct: 2041 PWPQWPRIFRVDYGHQEAATKFGKDPRSYEVLTKRFIGDENGVVKGLEVIRVQWEKDANG 2100

Query: 2101 RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGI 2160
            RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFST+VDGI
Sbjct: 2101 RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTSVDGI 2160

Query: 2161 FAAGDCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQ 2220
            FAAGDCRRGQSLVVWAI+EGRQAAAQVDKY SKEEKN+LVGEGGYEGVG  SQDYNNRQQ
Sbjct: 2161 FAAGDCRRGQSLVVWAISEGRQAAAQVDKYLSKEEKNLLVGEGGYEGVGKSSQDYNNRQQ 2220

Query: 2221 D-STSSSRHTVMT 2229
            D S+SSSRHT+MT
Sbjct: 2221 DSSSSSSRHTIMT 2227

BLAST of MS000486 vs. NCBI nr
Match: XP_022922725.1 (glutamate synthase [NADH], amyloplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 4251.4 bits (11025), Expect = 0.0e+00
Identity = 2112/2234 (94.54%), Postives = 2176/2234 (97.40%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSL+KL AAPS+FDK+SVKP LNV  KAR+G RAARCSASK A+RSLNV+EKKFF
Sbjct: 1    MLAKSGSLIKLTAAPSSFDKSSVKPQLNVNSKARIGARAARCSASKAASRSLNVTEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSG +QFWH DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGGVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAG+L+ALPHE+FKE      AA+D
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGLLLALPHEFFKE------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESK+VF KVAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKKVFAKVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            L TEPVIEQVFLTPSTRSKVDLEKQMYILRRLS+VAI+AALNLEHGGARDFYICSLSSRT
Sbjct: 241  LQTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSVVAIQAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKP+QLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPIQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMD QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDAQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK+QKIELKD+VGS+DKSE+T P IAGALPTS D+DNME MGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKRQKIELKDVVGSIDKSELTTPSIAGALPTSADDDNMETMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLPFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPL+SIE+MEAIK+MNYRGWRSKV+DITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLVSIEEMEAIKQMNYRGWRSKVVDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAI+EGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIREGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIPAKS+GE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPAKSNGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVE ASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGTSIPLDEVEAASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM  LPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMESLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMS+LG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSKLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKSLPVYIE+PINNVNRAVGTMLSHEVTKRYHMAGLP ETIHIKF GS
Sbjct: 1381 DQKLIALSKSALEKSLPVYIESPINNVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFNGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGI LELEGDSNDYVGKGLSGGKIVVYPP+ SLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRRSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDI+ LKMMIQQHQRHTSSNLAKEVL+NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDIVALKMMIQQHQRHTSSNLAKEVLDNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKRVLANMKAQ A KE SEPAAK+ EEESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRVLANMKAQGATKEVSEPAAKDVEEESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQ----VEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES 1740
            LNG+SKQ    VE+TEPPKRPTE  DAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES
Sbjct: 1681 LNGDSKQLDPKVEKTEPPKRPTETLDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES 1740

Query: 1741 KPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFP 1800
            KPGPLLKTQSARCMDCGTPFCHQE+SGCPLGNKIPEFNELVYQNRWREALERLLETNNFP
Sbjct: 1741 KPGPLLKTQSARCMDCGTPFCHQESSGCPLGNKIPEFNELVYQNRWREALERLLETNNFP 1800

Query: 1801 EFTGRVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVG 1860
            EFTGRVCPAPCEGSCVLGIIENPVSIK+IECAIIDKAFEEGWM PRPPLTRSGKRVAI+G
Sbjct: 1801 EFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMTPRPPLTRSGKRVAIIG 1860

Query: 1861 SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN 1920
            SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN
Sbjct: 1861 SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN 1920

Query: 1921 FVVNANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKS 1980
            FVVNAN+GTDPSY+LDRL+DENDALVLAVGATKPRDLPVPGREL+GVHFAMEFLHSNTKS
Sbjct: 1921 FVVNANVGTDPSYSLDRLQDENDALVLAVGATKPRDLPVPGRELSGVHFAMEFLHSNTKS 1980

Query: 1981 LLDSNLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGN 2040
            LLDSNL++G YISAKDKKVVVIGGGDTGTDCIGTSIRHGCS+VVNLELLPQPPQTRAPGN
Sbjct: 1981 LLDSNLQDGKYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSVVNLELLPQPPQTRAPGN 2040

Query: 2041 PWPQWPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANG 2100
            PWPQWPRIFRVDYGHQEAA KFGKDPRSYEVLTKRFIGDENG VKGLE++RVQWEKDANG
Sbjct: 2041 PWPQWPRIFRVDYGHQEAATKFGKDPRSYEVLTKRFIGDENGVVKGLEVIRVQWEKDANG 2100

Query: 2101 RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGI 2160
            RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFST+VDGI
Sbjct: 2101 RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTSVDGI 2160

Query: 2161 FAAGDCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQ 2220
            FAAGDCRRGQSLVVWAI+EGRQAAAQVDKY SKEEKN+LVGEGGYEGVG  SQDYNNRQQ
Sbjct: 2161 FAAGDCRRGQSLVVWAISEGRQAAAQVDKYLSKEEKNLLVGEGGYEGVGKSSQDYNNRQQ 2220

Query: 2221 D--STSSSRHTVMT 2229
            D  S+SSSRHT+MT
Sbjct: 2221 DSSSSSSSRHTIMT 2228

BLAST of MS000486 vs. ExPASy Swiss-Prot
Match: Q03460 (Glutamate synthase [NADH], amyloplastic OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 3727.2 bits (9664), Expect = 0.0e+00
Identity = 1835/2210 (83.03%), Postives = 2009/2210 (90.90%), Query Frame = 0

Query: 25   PHLNV--TPKARLG--TRAARCSASKGATRSLNVSEKKFFGARLRAPGS-GRIQFWHSDG 84
            P +N    P ARL    R  RCSA+        V  K++ G +LR+ G   RIQ W S G
Sbjct: 15   PQINAISNPNARLRPLARVTRCSAT-------CVERKRWLGTKLRSGGGLERIQLWESGG 74

Query: 85   PGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEMLV 144
             GR PKLR+ V+S  SAVP+KP+GLYDP+FDKDSCGVGFVAEL+G+SSRKT+TDALEMLV
Sbjct: 75   LGRLPKLRVAVKSSFSAVPDKPMGLYDPAFDKDSCGVGFVAELNGQSSRKTVTDALEMLV 134

Query: 145  RMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKDNGFELPPPGQYAVGMFFLP 204
            RM+HRGACGCE NTGDGAGILVALPH +++E            F+LPP G YAVGMFFLP
Sbjct: 135  RMTHRGACGCEANTGDGAGILVALPHGFYQEV---------VDFQLPPQGNYAVGMFFLP 194

Query: 205  TSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTRSK 264
             SDSRR+ESK +FTKVAESLGH VLGWRSV TDNTGLGKSA LTEPVIEQVFLTPS+ SK
Sbjct: 195  KSDSRRKESKNIFTKVAESLGHKVLGWRSVPTDNTGLGKSAQLTEPVIEQVFLTPSSDSK 254

Query: 265  VDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKD-YYLD 324
            VDLEKQMYILR+LSMV+I +ALNL+  G  DFYICSLSSRT++YKGQL P QL + YY D
Sbjct: 255  VDLEKQMYILRKLSMVSITSALNLQSDGITDFYICSLSSRTVIYKGQLTPAQLGEYYYAD 314

Query: 325  LGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLKCQ 384
            LGNERFTSYMALIHSRFSTNTFPSWDRAQP RVLGHNGEINTLRGNVNW+KAREGLLKC+
Sbjct: 315  LGNERFTSYMALIHSRFSTNTFPSWDRAQPFRVLGHNGEINTLRGNVNWIKAREGLLKCK 374

Query: 385  ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMDP 444
            ELGLSE++LK  LPIVDA+SSDSG FDGVLE L+ +G+SLPEAVMMMIPEAWQNDKNMDP
Sbjct: 375  ELGLSENDLKKFLPIVDANSSDSGCFDGVLEFLLHSGKSLPEAVMMMIPEAWQNDKNMDP 434

Query: 445  QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEVGV 504
            QRKA YEY+S LMEPWDGPALISFTDG YLGATLDRNGLRPGRFYVTHSGRVIMASEVGV
Sbjct: 435  QRKAFYEYYSALMEPWDGPALISFTDGHYLGATLDRNGLRPGRFYVTHSGRVIMASEVGV 494

Query: 505  VDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKDIV 564
            VDI PEDV RKGRLNPGMMLLVDFE  +VV+D+ALK+QYSLARPYG+WL+KQKIELKDI+
Sbjct: 495  VDIPPEDVCRKGRLNPGMMLLVDFEKQIVVNDDALKEQYSLARPYGDWLEKQKIELKDII 554

Query: 565  GSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKDGV 624
             SV +S++ PP I+G  P S D+ +MENMGI GLLAPLKAFGY+ E+LE+LLLPMAKDGV
Sbjct: 555  DSVHESDIVPPTISGVPPLSNDDVDMENMGIQGLLAPLKAFGYSVESLEILLLPMAKDGV 614

Query: 625  EALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGDLT 684
            EALGSMGNDTPLAVMS+REKLTFEYFKQMFAQVTNPPIDPIREKIVTSM+CM+GPEGDLT
Sbjct: 615  EALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMRCMVGPEGDLT 674

Query: 685  ETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITYPKYLGRRGLEETLDRICA 744
            ETTEEQCHRLSLKGPLLS ++MEAIK+MNYRGWRSKV+DITY K  G +GLEE LDRIC 
Sbjct: 675  ETTEEQCHRLSLKGPLLSTKEMEAIKKMNYRGWRSKVIDITYSKERGTKGLEEALDRICT 734

Query: 745  EAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEPRE 804
            EAH+AI EGYTTLVLSDRAFS K VAVSSLLAVGAVHQ+LV  LERT+V L++ESAEPRE
Sbjct: 735  EAHNAISEGYTTLVLSDRAFSKKHVAVSSLLAVGAVHQHLVKTLERTRVALMVESAEPRE 794

Query: 805  VHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELHTKEELVKKYFKASNYGMM 864
            VHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIP K+SG+ ++K+ELVKKYFKAS YGMM
Sbjct: 795  VHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPPKASGDFNSKDELVKKYFKASTYGMM 854

Query: 865  KVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHEMA 924
            KVLAKMGISTLASYKGAQIFEALGLSSEV+EKCFAGTPSRVEGATFEMLA+DAL+LHE+A
Sbjct: 855  KVLAKMGISTLASYKGAQIFEALGLSSEVIEKCFAGTPSRVEGATFEMLAQDALHLHELA 914

Query: 925  FPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSKLV 984
            FP+R+F  GSAEAVALPNPGDYHWRKGGEVHLNDP+AIAKLQEAARTNSV+AYK+YSK +
Sbjct: 915  FPSRIFSPGSAEAVALPNPGDYHWRKGGEVHLNDPLAIAKLQEAARTNSVDAYKQYSKTI 974

Query: 985  HELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMN 1044
            HELNKACNLRGLLKFK+    +P+ EVEPASEIVKRFCTGAMSYGSISLEAHT LA AMN
Sbjct: 975  HELNKACNLRGLLKFKDAASKVPISEVEPASEIVKRFCTGAMSYGSISLEAHTALATAMN 1034

Query: 1045 KIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQ 1104
             IGGKSNTGEGGEQPSRM PL DGS NPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQ
Sbjct: 1035 TIGGKSNTGEGGEQPSRMEPLADGSRNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQ 1094

Query: 1105 GAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPAA 1164
            GAKPGEGGELPGHKV+G+IA TRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN+NPAA
Sbjct: 1095 GAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANPAA 1154

Query: 1165 RISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQ 1224
            RISVKLVSEAGVGVIASGVVKGHA+HVLISGHDGGTGASRWTGIK+AGLPWELGLAETHQ
Sbjct: 1155 RISVKLVSEAGVGVIASGVVKGHAEHVLISGHDGGTGASRWTGIKSAGLPWELGLAETHQ 1214

Query: 1225 TLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRKCHKNTCP 1284
            TLVANDLRGRT LQTDGQLKTGRDVAIAALLGAEE+GFSTAPLIT+GCIMMRKCHKNTCP
Sbjct: 1215 TLVANDLRGRTTLQTDGQLKTGRDVAIAALLGAEEYGFSTAPLITLGCIMMRKCHKNTCP 1274

Query: 1285 VGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDLLEVDKEV 1344
            VGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLG RTVN+MVGRSD+LEVDKEV
Sbjct: 1275 VGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGFRTVNEMVGRSDMLEVDKEV 1334

Query: 1345 AWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMALDQKLIALSKSAIEKSLPV 1404
               N KLENIDLSLLLRPAA+LRPEAAQ+CVQKQDHGLDMALD KLI+LS +A+EK LPV
Sbjct: 1335 VKGNAKLENIDLSLLLRPAAELRPEAAQYCVQKQDHGLDMALDNKLISLSNAALEKGLPV 1394

Query: 1405 YIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAFLCPGIMLELE 1464
            YIETPI N NRAVGTMLSHEVTKRY++AGLP +TIHI+FTGSAGQS GAFLCPGI LELE
Sbjct: 1395 YIETPICNTNRAVGTMLSHEVTKRYNLAGLPADTIHIQFTGSAGQSFGAFLCPGITLELE 1454

Query: 1465 GDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFNGMAAERFCVR 1524
            GDSNDY+GKGLSGGK+VVYPPKGS FDPK+NI+IGNVALYGAT GEAYFNGMAAERFCVR
Sbjct: 1455 GDSNDYIGKGLSGGKVVVYPPKGSNFDPKDNILIGNVALYGATRGEAYFNGMAAERFCVR 1514

Query: 1525 NSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDGKFESRCNLEL 1584
            NSGA AVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+AYVLD+DG F+SRCNLEL
Sbjct: 1515 NSGALAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGTFQSRCNLEL 1574

Query: 1585 VDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFPREYKRVLANM 1644
            VDLDKVEEE+DI+TL+M+IQQHQRHT+S LAKEVL +FENLLP+F+KVFPREYKRVLA+M
Sbjct: 1575 VDLDKVEEEEDIITLRMLIQQHQRHTNSLLAKEVLVDFENLLPKFVKVFPREYKRVLASM 1634

Query: 1645 KAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAASLNGNSKQVEETEPPKRPT 1704
            K+  A+K+A E AA++ +E+ DEA+ +EKDAFEELKK+A ASLN      + +E PKRP+
Sbjct: 1635 KSDAASKDAVERAAEDVDEQDDEAQAVEKDAFEELKKLATASLN-----EKPSEAPKRPS 1694

Query: 1705 EIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGPLLKTQSARCMDCGTPFCH 1764
            ++ DAVKHRGF+AYEREGV+YRDPNVR+ DW EVM E+KPGPLLKTQSARCMDCGTPFCH
Sbjct: 1695 QVTDAVKHRGFVAYEREGVQYRDPNVRLNDWNEVMMETKPGPLLKTQSARCMDCGTPFCH 1754

Query: 1765 QENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTGRVCPAPCEGSCVLGIIEN 1824
            QENSGCPLGNKIPEFNELVYQNRW+EALERLLETNNFPEFTGRVCPAPCEGSCVLGIIEN
Sbjct: 1755 QENSGCPLGNKIPEFNELVYQNRWQEALERLLETNNFPEFTGRVCPAPCEGSCVLGIIEN 1814

Query: 1825 PVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPAGLAAADQLNKMGHTVTVY 1884
            PVSIKNIECAIIDKAFEEGWM+PRPP+ R+GKRVAIVGSGP+GLAAADQLNKMGH VTV+
Sbjct: 1815 PVSIKNIECAIIDKAFEEGWMIPRPPVKRTGKRVAIVGSGPSGLAAADQLNKMGHIVTVF 1874

Query: 1885 ERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVNANIGTDPSYTLDRLRDEN 1944
            ERADRIGGLMMYGVPNMKTDKVD+VQRRVNLMAEEG+NFVVNANIG DP Y+L+RLR+EN
Sbjct: 1875 ERADRIGGLMMYGVPNMKTDKVDIVQRRVNLMAEEGINFVVNANIGLDPLYSLERLREEN 1934

Query: 1945 DALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDSNLENGNYISAKDKKVVVI 2004
            DA+VLAVGATKPRDLPVPGREL+GVHFAMEFLH+NTKSLLDSNL++GNYISAK KKVVVI
Sbjct: 1935 DAIVLAVGATKPRDLPVPGRELSGVHFAMEFLHANTKSLLDSNLQDGNYISAKGKKVVVI 1994

Query: 2005 GGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQWPRIFRVDYGHQEAAAKF 2064
            GGGDTGTDCIGTSIRHGC+ VVNLELLPQPP TRAPGNPWPQWPRIFRVDYGHQEA  KF
Sbjct: 1995 GGGDTGTDCIGTSIRHGCTAVVNLELLPQPPPTRAPGNPWPQWPRIFRVDYGHQEAETKF 2054

Query: 2065 GKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQFKEVEGSEEIIEADLVLLA 2124
            GKDPR+YEVLTKRF+GDENG VKGLE+VRV WEKD  G+FQFKE+EGSEEIIEADLVLLA
Sbjct: 2055 GKDPRTYEVLTKRFVGDENGVVKGLEVVRVCWEKDETGKFQFKEIEGSEEIIEADLVLLA 2114

Query: 2125 MGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAGDCRRGQSLVVWAINEGRQ 2184
            MGFLGPE+T+AEKLGVE+DNRSNFKA+YGRFST+VDG+FAAGDCRRGQSLVVWAI+EGRQ
Sbjct: 2115 MGFLGPEATIAEKLGVERDNRSNFKADYGRFSTSVDGVFAAGDCRRGQSLVVWAISEGRQ 2174

Query: 2185 AAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTSSSRHTVMT 2229
            AAAQVD Y + E+  I   +G  +    R QD N +       S+HTVMT
Sbjct: 2175 AAAQVDSYLTNEDHGI---DGNQDEFVKRQQDLNKKH------SKHTVMT 2194

BLAST of MS000486 vs. ExPASy Swiss-Prot
Match: Q9LV03 (Glutamate synthase 1 [NADH], chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GLT1 PE=1 SV=2)

HSP 1 Score: 3702.5 bits (9600), Expect = 0.0e+00
Identity = 1822/2179 (83.62%), Postives = 1988/2179 (91.23%), Query Frame = 0

Query: 19   DKTSVKPHLNVTP-KARLGTRAARCSASKGATRSLNVSEKKFFGARLRAPGSGRIQFWHS 78
            + TSV   L VT   +R  +  ARCS  K       + E  F G R+R  GS  +QFW S
Sbjct: 26   NSTSVASQLAVTSGVSRRRSCTARCSVKKPV-----IPESPFLGTRVRRSGSETLQFWRS 85

Query: 79   DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEM 138
            DGPGRS KLR VV+S  SAVPEKPLGLYDPS+DKDSCGVGFVAELSGE++RKT+TD+LEM
Sbjct: 86   DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEM 145

Query: 139  LVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKDNGFELPPPGQYAVGMFF 198
            L+RM+HRGACGCE+NTGDGAGILV LPH+++ E      AA + GF LP  G YAVGMFF
Sbjct: 146  LIRMTHRGACGCESNTGDGAGILVGLPHDFYAE------AATELGFVLPSAGNYAVGMFF 205

Query: 199  LPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTR 258
            LPT +SRREESK VFTKVAESLGHSVLGWR V TDN+GLG SAL TEP+I QVFLTP+T+
Sbjct: 206  LPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTK 265

Query: 259  SKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKD-YY 318
            SK D E+QMYILRR+SMVAIRAALNL+HG  +DFYICSLSSRTIVYKGQLKP QLKD YY
Sbjct: 266  SKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYY 325

Query: 319  LDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLK 378
             DLG+ERFTSYMAL+HSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWM+AREGLLK
Sbjct: 326  ADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLK 385

Query: 379  CQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNM 438
            C ELGLS+ ELK LLPIVD SSSDSGAFDGVLELL+RAGRSLPEAVMMMIPEAWQNDKN+
Sbjct: 386  CNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNI 445

Query: 439  DPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEV 498
            DP RK  YEY S LMEPWDGPALISFTDGRYLGATLDRNGLRPGRFY+THSGRVIMASEV
Sbjct: 446  DPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEV 505

Query: 499  GVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKD 558
            GVVD+ PEDV RKGRLNPGMMLLVDFE H+VVDD+ALKQQYSLARPYGEWLK+QKIELKD
Sbjct: 506  GVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKD 565

Query: 559  IVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKD 618
            I+ SV ++E   P I+G +P S D+D+ME+MGIHGLL+PLKAFGYT EALEMLLLPMAKD
Sbjct: 566  IIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKD 625

Query: 619  GVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGD 678
            G EALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIREKIVTSM+CMIGPEGD
Sbjct: 626  GSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGD 685

Query: 679  LTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITYPKYLGRRGLEETLDRI 738
            LTETTEEQCHRLSLKGPLL IE+MEAIK+MNYRGWR+KVLDITY K  G +GLEETLDRI
Sbjct: 686  LTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRI 745

Query: 739  CAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEP 798
            C EA++AIKEGYT LVLSDRAFS  RVAVSSL+AVGAVH +LV  L RTQVGL++ESAEP
Sbjct: 746  CDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEP 805

Query: 799  REVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELHTKEELVKKYFKASNYG 858
            REVHHFCTLVGFGADAICPYLAVEA++RLQVDGKIP KS+GE H+KEELVKKY+KASNYG
Sbjct: 806  REVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYG 865

Query: 859  MMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHE 918
            MMKVLAKMGISTLASYKGAQIFEALGLSSEV++KCFAGTPSRVEGATFEMLARD L LHE
Sbjct: 866  MMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHE 925

Query: 919  MAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSK 978
            +AFPTR +  GSAEA AL NPG+YHWRK GE+HLNDP+AIAKLQEAARTNSV AYKEYSK
Sbjct: 926  LAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSK 985

Query: 979  LVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1038
             ++ELNK  NLRGL+KFK+  V IPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA
Sbjct: 986  RINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1045

Query: 1039 MNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKM 1098
            MNK+GGKSNTGEGGE PSRM PL DGS NPKRS+IKQ+ASGRFGVSSYYLTNADELQIKM
Sbjct: 1046 MNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKM 1105

Query: 1099 AQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNP 1158
            AQGAKPGEGGELPGHKV+G+IA TRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN+NP
Sbjct: 1106 AQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANP 1165

Query: 1159 AARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAET 1218
             ARISVKLVSEAGVGVIASGVVKGHADHVLI+GHDGGTGASRWTGIKNAGLPWELGLAET
Sbjct: 1166 GARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAET 1225

Query: 1219 HQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRKCHKNT 1278
            HQTLVANDLRGRTVLQTDGQLKTGRDVA+AALLGAEEFGFSTAPLIT+GCIMMRKCHKNT
Sbjct: 1226 HQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNT 1285

Query: 1279 CPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDLLEVDK 1338
            CPVGIATQDPVLREKFAGEPEHVINFFFM+AEE+REIMS LG RTV +M+GR+D+LE+D+
Sbjct: 1286 CPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDR 1345

Query: 1339 EVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMALDQKLIALSKSAIEKSL 1398
            EV   N+KLENIDLSLLLRPAA++RP AAQ+CVQKQDHGLDMALDQ+LIALSKSA+EKSL
Sbjct: 1346 EVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSL 1405

Query: 1399 PVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAFLCPGIMLE 1458
            PVYIETPI NVNRAVGTMLSHEVTKRYH+ GLP +TIHIKFTGSAGQSLGAFLCPGIMLE
Sbjct: 1406 PVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLE 1465

Query: 1459 LEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFNGMAAERFC 1518
            LEGDSNDYVGKGLSGGK+VVYPPKGS FDPKENI+IGNVALYGATSGEAYFNGMAAERF 
Sbjct: 1466 LEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFS 1525

Query: 1519 VRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDGKFESRCNL 1578
            VRNSGAKAVVEG+GDHGCEYMTGGTVVVLG TGRNFAAGMSGG+AYVLD+DGKF +RCNL
Sbjct: 1526 VRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNL 1585

Query: 1579 ELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFPREYKRVLA 1638
            ELVDLDKVE+E+D +TLKMMIQQHQRHT+S LA+EVL +FENLLP+FIKVFPR+YKRVL+
Sbjct: 1586 ELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLS 1645

Query: 1639 NMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS----LNGNSKQVEETE 1698
             MK +E +K+A E A++EA +E++E EL EKDAF ELK MAAAS    ++GN    E   
Sbjct: 1646 AMKHEEVSKQAIERASEEA-DETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAE--- 1705

Query: 1699 PPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGPLLKTQSARCMDC 1758
               RP+++ +AVK+ GFIAYEREGVKYRDPNVR+ DW EVMEESKPGPLL TQSARCMDC
Sbjct: 1706 --ARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDC 1765

Query: 1759 GTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTGRVCPAPCEGSCV 1818
            GTPFCHQENSGCPLGNKIPEFNELVYQNRW+EAL RLLETNNFPEFTGRVCPAPCEGSCV
Sbjct: 1766 GTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCV 1825

Query: 1819 LGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPAGLAAADQLNKMG 1878
            LGIIENPVSIK+IECAIIDKAFEEGWMVPRPPL R+GK+VAI+GSGPAGLAAADQLNKMG
Sbjct: 1826 LGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMG 1885

Query: 1879 HTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVNANIGTDPSYTLD 1938
            H VTVYER+DRIGGLMMYGVPNMKTDK+DVVQRRV+LM +EG+NFVVNANIG DPSY+LD
Sbjct: 1886 HLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLD 1945

Query: 1939 RLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDSNLENGNYISAKD 1998
             L++ENDA+VLAVG+TKPRDLPVPGR+L+GVHFAMEFLH+NTKSLLDSN E+GNYISAK 
Sbjct: 1946 GLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKG 2005

Query: 1999 KKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQWPRIFRVDYGHQ 2058
            KKVVVIGGGDTGTDCIGTSIRHGC+N+VNLELLPQPP TRAPGNPWPQWPR+FR+DYGHQ
Sbjct: 2006 KKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQ 2065

Query: 2059 EAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQFKEVEGSEEIIEA 2118
            EA  KFGKDPR+YEVLTKRFIGD+NG VKGLELVRV WEKD  GRFQFKE+EGSEEIIEA
Sbjct: 2066 EATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEA 2125

Query: 2119 DLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAGDCRRGQSLVVWA 2178
            DLV LAMGFLGPE T+AEKLG+E DNRSNFKAEYGRFST V+G+FAAGDCRRGQSLVVWA
Sbjct: 2126 DLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWA 2185

Query: 2179 INEGRQAAAQVDKYFSKEE 2192
            I+EGRQAA QVDK+ +K +
Sbjct: 2186 ISEGRQAADQVDKFLTKTD 2187

BLAST of MS000486 vs. ExPASy Swiss-Prot
Match: Q0JKD0 (Glutamate synthase 1 [NADH], chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0681900 PE=2 SV=1)

HSP 1 Score: 3416.3 bits (8857), Expect = 0.0e+00
Identity = 1692/2176 (77.76%), Postives = 1886/2176 (86.67%), Query Frame = 0

Query: 34   RLGTRAARCSASKGATRSLNVSEKKFFGARLRAPG-------SGRIQFWHSDGPGRSPKL 93
            +L T AA   A + A RS +     +  ARL   G        G  Q        R P  
Sbjct: 10   KLRTDAAPTGAGRRARRSHSSVAAPYRAARLVQGGVSIEGGLVGGCQLTEERVAARPP-- 69

Query: 94   RLVVRSG-----LSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEMLVRM 153
            R   R       LS +PE  +GLYDPS ++DSCGVGFVAELSG+  R T+ DALEML RM
Sbjct: 70   RAAARDAEPVRPLSTLPESSIGLYDPSRERDSCGVGFVAELSGDYKRATVNDALEMLERM 129

Query: 154  SHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKDNGFELPPPGQYAVGMFFLPTS 213
            +HRGACGCE NTGDGAGILVALPH +F+E        KD GFELP PG+YAVGM FLP  
Sbjct: 130  AHRGACGCEKNTGDGAGILVALPHNFFRE------VTKDAGFELPQPGEYAVGMVFLPID 189

Query: 214  DSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTRSKVD 273
            + RRE SK  F KVAESLGH +LGWR V TDN+ LG+SAL TEPVIEQVFLT S+ S+ D
Sbjct: 190  EKRRERSKAEFQKVAESLGHVILGWRRVPTDNSDLGESALQTEPVIEQVFLTKSSSSEAD 249

Query: 274  LEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKD-YYLDLG 333
             E+Q+YILRRLS+++IRAALNL  GG RDFY+CSLSSRTIVYKGQLKP QLK  YY DLG
Sbjct: 250  FEQQLYILRRLSILSIRAALNLRRGGKRDFYMCSLSSRTIVYKGQLKPCQLKGYYYADLG 309

Query: 334  NERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLKCQEL 393
            +E FTSYMAL+HSRFSTNTFPSWDRAQPMRVLGHNGEINTL+GN NWMKAREGLL+C++L
Sbjct: 310  HENFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLKGNKNWMKAREGLLECEKL 369

Query: 394  GLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMDPQR 453
            GL++D+   +LPIVDA+SSDSGAFDGVLELLIR GRSLPEAVMMMIPEAWQND NM+P++
Sbjct: 370  GLTKDQFSKILPIVDATSSDSGAFDGVLELLIRGGRSLPEAVMMMIPEAWQNDVNMEPEK 429

Query: 454  KALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVD 513
            KALYE+ S LMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRV+M SEVGVVD
Sbjct: 430  KALYEFLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVVMGSEVGVVD 489

Query: 514  IAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKDIVGS 573
            +  +DV RKGRLNPGMMLLVDFENH VVDDEALK QYS A PYGEWLK+QKI LKDIV S
Sbjct: 490  VPSKDVLRKGRLNPGMMLLVDFENHTVVDDEALKAQYSKAHPYGEWLKRQKIYLKDIVES 549

Query: 574  VDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKDGVEA 633
            V ++E   P I+G+L  +   +  E+ G++G++ PLKAFGYT EALEMLLLPMAKDGVEA
Sbjct: 550  VPETERVAPGISGSL--TQKNEKKEHAGVNGIVTPLKAFGYTVEALEMLLLPMAKDGVEA 609

Query: 634  LGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGDLTET 693
            LGSMGNDTPLAVMS+REKLTFEYFKQMFAQVTNPPIDPIREKIVTSM+CMIGPEGDL ET
Sbjct: 610  LGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGDLLET 669

Query: 694  TEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITYPKYLGRRGLEETLDRICAEA 753
            TE+QC+RL+L+GPL+SI++MEAIK+MNYRGWRSKVLDITYPK  GR+GLEETLDRIC EA
Sbjct: 670  TEKQCNRLALEGPLVSIDEMEAIKKMNYRGWRSKVLDITYPKKSGRKGLEETLDRICTEA 729

Query: 754  HDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEPREVH 813
              AIK+GYT LVLSDR FS+ RVAVSSLLAVGAVHQ+LV NLERT+VGL++ESAEPREVH
Sbjct: 730  RGAIKKGYTVLVLSDRGFSSDRVAVSSLLAVGAVHQHLVANLERTRVGLLVESAEPREVH 789

Query: 814  HFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELHTKEELVKKYFKASNYGMMKV 873
            HFCTLVGFGADA+CPYLA+EAIW LQ DGKIP    G+ ++KEELVKKYF ASNYGMMKV
Sbjct: 790  HFCTLVGFGADAVCPYLAIEAIWCLQNDGKIPPNGDGKPYSKEELVKKYFYASNYGMMKV 849

Query: 874  LAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHEMAFP 933
            LAKMGISTLASYKGAQIFEALGLSSEV+ KCF GTPSR+EGATFEMLARDAL LHE+AFP
Sbjct: 850  LAKMGISTLASYKGAQIFEALGLSSEVIRKCFDGTPSRIEGATFEMLARDALRLHELAFP 909

Query: 934  TRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSKLVHE 993
            +R  P GSA+A ALPNPGDYHWRK GEVHLNDP+A+AKLQEAAR NS  AYKEYS+ + E
Sbjct: 910  SRAPPPGSADAKALPNPGDYHWRKNGEVHLNDPLAMAKLQEAARVNSRAAYKEYSRRIQE 969

Query: 994  LNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKI 1053
            LNK CNLRG+LKFK+T   I +DEVEPASEIVKRF TGAMSYGSISLEAHT LAMAMNK+
Sbjct: 970  LNKTCNLRGMLKFKDTADMISVDEVEPASEIVKRFVTGAMSYGSISLEAHTALAMAMNKL 1029

Query: 1054 GGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGA 1113
            GGKSNTGEGGEQPSRM PL +GSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGA
Sbjct: 1030 GGKSNTGEGGEQPSRMEPLANGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKMAQGA 1089

Query: 1114 KPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPAARI 1173
            KPGEGGELPGHKV+G+IA TR+STAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNP ARI
Sbjct: 1090 KPGEGGELPGHKVIGDIAVTRHSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPRARI 1149

Query: 1174 SVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTL 1233
            SVKLVSEAGVGV+ASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTL
Sbjct: 1150 SVKLVSEAGVGVVASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAETHQTL 1209

Query: 1234 VANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRKCHKNTCPVG 1293
            VAN LRGR +LQTDGQLKTG+DVA+A LLGAEEFGFSTAPLIT+GCIMMRKCH NTCPVG
Sbjct: 1210 VANGLRGRAILQTDGQLKTGKDVAVACLLGAEEFGFSTAPLITLGCIMMRKCHTNTCPVG 1269

Query: 1294 IATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDLLEVDKEVAW 1353
            IATQDPVLREKFAGEPEHVINFFFM+AEE+REIMSQLG RT+ +MVGRSD+LEVD EV  
Sbjct: 1270 IATQDPVLREKFAGEPEHVINFFFMLAEELREIMSQLGFRTITEMVGRSDMLEVDPEVVK 1329

Query: 1354 QNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMALDQKLIALSKSAIEKSLPVYI 1413
             NEKLENIDLSL+L+PAA++RP AAQ+CV+KQDHGLDMALD KLIALSK+A+EK + V+I
Sbjct: 1330 SNEKLENIDLSLILKPAAEIRPGAAQYCVEKQDHGLDMALDNKLIALSKAALEKEVRVFI 1389

Query: 1414 ETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAFLCPGIMLELEGD 1473
            ETPI N NRAVGTMLSHEVTKRYHM GLP  TIH+K TGSAGQSLGAFLCPGI LELEGD
Sbjct: 1390 ETPIQNTNRAVGTMLSHEVTKRYHMKGLPAGTIHVKLTGSAGQSLGAFLCPGITLELEGD 1449

Query: 1474 SNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFNGMAAERFCVRNS 1533
            SNDYVGKGLSGGKIVVYPP+ S F P++NI+IGNVALYGAT GEAYFNGMAAERFCVRNS
Sbjct: 1450 SNDYVGKGLSGGKIVVYPPRDSTFIPEDNIVIGNVALYGATIGEAYFNGMAAERFCVRNS 1509

Query: 1534 GAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDGKFESRCNLELVD 1593
            GA+AVVEG+GDHGCEYMTGGTVV+LG TGRNFAAGMSGG+AYV DIDGKF  RCN ELVD
Sbjct: 1510 GAQAVVEGIGDHGCEYMTGGTVVILGKTGRNFAAGMSGGIAYVYDIDGKFSVRCNHELVD 1569

Query: 1594 LDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFPREYKRVLANMKA 1653
            L  VEEE+DI TLKMMI+QH+ +T S +A+++L NF+ LLP+F+KVFPR+YKRVL NMKA
Sbjct: 1570 LYHVEEEEDITTLKMMIEQHRLNTGSVVARDILSNFDTLLPKFVKVFPRDYKRVLDNMKA 1629

Query: 1654 QE-AAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAASLNGNSKQVEETEPPKRPTE 1713
            ++ AAK A EP                              ++  +K+V+  +   RPT 
Sbjct: 1630 EKAAAKLAKEPKISN-------------------------GVSVTTKKVQPEQSTNRPTR 1689

Query: 1714 IPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGPLLKTQSARCMDCGTPFCHQ 1773
            + +A K+RGFI+YERE + YRDPN R+ DWKEV  ES PGPLL TQSARCMDCGTPFCHQ
Sbjct: 1690 VSNAKKYRGFISYERESISYRDPNERVKDWKEVAIESVPGPLLNTQSARCMDCGTPFCHQ 1749

Query: 1774 ENS--GCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTGRVCPAPCEGSCVLGIIE 1833
            E+S  GCPLGNKIPEFNELV+QNRWREAL+RLLETNNFPEFTGRVCPAPCEGSCVLGIIE
Sbjct: 1750 ESSGAGCPLGNKIPEFNELVHQNRWREALDRLLETNNFPEFTGRVCPAPCEGSCVLGIIE 1809

Query: 1834 NPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPAGLAAADQLNKMGHTVTV 1893
            NPVSIK+IECAIIDK FEEGWMVPRPPL R+GK+VAI+GSGPAGLAAADQLNKMGH VTV
Sbjct: 1810 NPVSIKSIECAIIDKGFEEGWMVPRPPLQRTGKKVAIIGSGPAGLAAADQLNKMGHFVTV 1869

Query: 1894 YERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVNANIGTDPSYTLDRLRDE 1953
            +ERADRIGGLMMYGVPNMKTDK+++VQRRVNLMAEEG+ FVVNAN+G+DP Y+++RLR E
Sbjct: 1870 FERADRIGGLMMYGVPNMKTDKIEIVQRRVNLMAEEGITFVVNANVGSDPLYSIERLRSE 1929

Query: 1954 NDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDSNLENGNYISAKDKKVVV 2013
            NDA++LA GATKPRDL +PGREL+GVHFAMEFLH+NTKSLLDSNLE+G YISAK KKVVV
Sbjct: 1930 NDAVILACGATKPRDLGIPGRELSGVHFAMEFLHANTKSLLDSNLEDGRYISAKGKKVVV 1989

Query: 2014 IGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQWPRIFRVDYGHQEAAAK 2073
            IGGGDTGTDCIGTSIRHGC+++VNLELL +PP  RA  NPWPQWPRIFRVDYGHQEA++K
Sbjct: 1990 IGGGDTGTDCIGTSIRHGCTSIVNLELLTKPPSKRAADNPWPQWPRIFRVDYGHQEASSK 2049

Query: 2074 FGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQFKEVEGSEEIIEADLVLL 2133
            FG DPR+YEVLTKRFIGDENG VK LE+VRV+WEK  +GRFQFKE+EGS E IEADLVLL
Sbjct: 2050 FGNDPRTYEVLTKRFIGDENGNVKALEVVRVKWEK-VDGRFQFKEIEGSNETIEADLVLL 2109

Query: 2134 AMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAGDCRRGQSLVVWAINEGR 2193
            AMGFLGPE+T+AEKLG+EKDNRSNFKA++G F+T+VDGIFAAGDCRRGQSLVVWAI EGR
Sbjct: 2110 AMGFLGPEATIAEKLGLEKDNRSNFKAQFGNFATSVDGIFAAGDCRRGQSLVVWAITEGR 2149

BLAST of MS000486 vs. ExPASy Swiss-Prot
Match: Q0DG35 (Glutamate synthase 2 [NADH], chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0555600 PE=2 SV=2)

HSP 1 Score: 3380.9 bits (8765), Expect = 0.0e+00
Identity = 1668/2203 (75.71%), Postives = 1895/2203 (86.02%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            M A  G  LKL AAP+       K       +A   T AA      GA       E  F 
Sbjct: 1    MSAAQGLALKLRAAPAAGGVRGEKRR-----RAASATAAAAARPRHGAMS----LEGGFL 60

Query: 61   GARL--------RAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKD 120
            G  L        RA  S + +     G  R P         +S +PE  +GLYDPS ++D
Sbjct: 61   GGALPAEDRVAPRASASRQAEAGAGAGAARPPP------RSMSKIPESSIGLYDPSMERD 120

Query: 121  SCGVGFVAELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEAR 180
            SCGVGF+AELSGE SRKT+ DA+EML RM+HRGACGCE NTGDGAGILVALPH +F+E  
Sbjct: 121  SCGVGFIAELSGEYSRKTVDDAIEMLDRMAHRGACGCEKNTGDGAGILVALPHNFFRE-- 180

Query: 181  YILCAAKDNGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETD 240
                  KD GFELPPPG+YAVGMFF+PT D RRE+SK +F + AE LGH+VLGWR V TD
Sbjct: 181  ----VTKDAGFELPPPGEYAVGMFFMPTDDKRREKSKLLFREKAELLGHTVLGWRRVPTD 240

Query: 241  NTGLGKSALLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFY 300
            N+GLG+SA+ TEPVIEQVF+T S  SK D E+QMY+LRR S+++IR  L +++GG +DFY
Sbjct: 241  NSGLGQSAVDTEPVIEQVFVTKSASSKADFERQMYVLRRFSVMSIREVLGVKNGGTKDFY 300

Query: 301  ICSLSSRTIVYKGQLKPVQLKDYYL-DLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRV 360
            +CSLSSRTIVYKGQLKP QLK Y+  DLG+E FTSYMALIHSRFSTNTFPSWDRAQPMRV
Sbjct: 301  MCSLSSRTIVYKGQLKPSQLKGYFFADLGDESFTSYMALIHSRFSTNTFPSWDRAQPMRV 360

Query: 361  LGHNGEINTLRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELL 420
            LGHNGEINTLRGN NWMKAREGLLKC+ LGL+ DE+  LLPIVDA+SSDSGA D VLELL
Sbjct: 361  LGHNGEINTLRGNKNWMKAREGLLKCEGLGLTRDEMLKLLPIVDATSSDSGAIDNVLELL 420

Query: 421  IRAGRSLPEAVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGAT 480
            I++GRS PEAVMMMIPEAWQND NMDP+RKALYE+FS LMEPWDGPALISFTDGRYLGAT
Sbjct: 421  IQSGRSAPEAVMMMIPEAWQNDVNMDPERKALYEFFSALMEPWDGPALISFTDGRYLGAT 480

Query: 481  LDRNGLRPGRFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDE 540
            LDRNGLRPGRFYVT+SGRVIMASEVGVVD+ P+DVSRKGRLNPGMMLLVDFENH VV+D+
Sbjct: 481  LDRNGLRPGRFYVTYSGRVIMASEVGVVDVPPQDVSRKGRLNPGMMLLVDFENHCVVNDD 540

Query: 541  ALKQQYSLARPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHG 600
             LK++YS  RPYGEWLK+Q+I+L DI+ SV+++E   P I+GALP +  ++N  +MGI G
Sbjct: 541  ELKKEYSKVRPYGEWLKRQRIQLTDIIESVNEAERIAPSISGALPIT--KENKADMGICG 600

Query: 601  LLAPLKAFGYTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQV 660
            +L PLKAFGYT EALEML+LPMAKDG EALGSMGNDTPLAVMS+REKLTFEYFKQMFAQV
Sbjct: 601  ILTPLKAFGYTREALEMLMLPMAKDGQEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQV 660

Query: 661  TNPPIDPIREKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGW 720
            TNPPIDPIREKIVTSM+CMIGPEGDL+ETTE QCHRL+LK PLL+  +MEAIK+MNYRGW
Sbjct: 661  TNPPIDPIREKIVTSMECMIGPEGDLSETTERQCHRLTLKSPLLNTNEMEAIKKMNYRGW 720

Query: 721  RSKVLDITYPKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAV 780
            RSKVLDITYPK  GR GL++TLD+ICA+A +AI EGYT LVLSDR FS++RVAVSSLLAV
Sbjct: 721  RSKVLDITYPKKNGRMGLKQTLDKICAQAREAIHEGYTILVLSDRGFSSERVAVSSLLAV 780

Query: 781  GAVHQYLVNNLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKI 840
            GAVHQ+LV++LERT++GL++ESAEPREVHHF TL+GFGADAICPYLA+EAIWRLQ+DG+I
Sbjct: 781  GAVHQHLVSHLERTRIGLLVESAEPREVHHFSTLIGFGADAICPYLAIEAIWRLQIDGRI 840

Query: 841  PAKSSGELHTKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKC 900
            P  + G+ +T+E+L++KYF ASNYGMMKVLAKMGISTLASYKGAQIFEALGL+SEVV KC
Sbjct: 841  P-PNDGKPYTQEQLIEKYFYASNYGMMKVLAKMGISTLASYKGAQIFEALGLASEVVSKC 900

Query: 901  FAGTPSRVEGATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLN 960
            F GTPSRVEGATFEMLA+DAL LHE+AFP+R  P GSA+A ALPNPGDYHWRK GEVHLN
Sbjct: 901  FEGTPSRVEGATFEMLAQDALRLHEIAFPSRTLPPGSADANALPNPGDYHWRKNGEVHLN 960

Query: 961  DPVAIAKLQEAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEI 1020
            DP +IAKLQEAAR NS  AYKEYS+ ++ELNKAC LRG+LKF+E    I LDEVEPA EI
Sbjct: 961  DPFSIAKLQEAARINSREAYKEYSRRIYELNKACTLRGMLKFREIPNQISLDEVEPAKEI 1020

Query: 1021 VKRFCTGAMSYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAI 1080
            VKRFCTGAMSYGSISLEAHT+LA AMN +GGKSNTGEGGEQP RMVPLPDGS NP+ SAI
Sbjct: 1021 VKRFCTGAMSYGSISLEAHTSLAEAMNTLGGKSNTGEGGEQPCRMVPLPDGSKNPRISAI 1080

Query: 1081 KQVASGRFGVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLIS 1140
            KQVASGRFGVS YYLTNA E+QIKMAQGAKPGEGGELPGHKV+G+IA TRNSTAGVGLIS
Sbjct: 1081 KQVASGRFGVSIYYLTNAVEVQIKMAQGAKPGEGGELPGHKVIGDIAVTRNSTAGVGLIS 1140

Query: 1141 PPPHHDIYSIEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHD 1200
            PPPHHDIYSIEDLAQLIHDLKN+NP ARISVKLVSEAGVG++ASGVVKGHADHVLISGHD
Sbjct: 1141 PPPHHDIYSIEDLAQLIHDLKNANPGARISVKLVSEAGVGIVASGVVKGHADHVLISGHD 1200

Query: 1201 GGTGASRWTGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGA 1260
            GGTGASRWTGIKNAGLPWELGLAETHQTLVAN LRGR VLQTDGQ+KTGRDVA+A LLGA
Sbjct: 1201 GGTGASRWTGIKNAGLPWELGLAETHQTLVANGLRGRAVLQTDGQMKTGRDVAVACLLGA 1260

Query: 1261 EEFGFSTAPLITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMR 1320
            EEFGFSTAPLIT+GCIMMRKCH NTCP GIATQDPVLR KFAG+PEHVIN+FFM+AEE+R
Sbjct: 1261 EEFGFSTAPLITLGCIMMRKCHTNTCPAGIATQDPVLRAKFAGKPEHVINYFFMLAEEVR 1320

Query: 1321 EIMSQLGVRTVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQK 1380
            EIM+QLG RTVN+MVGRSD+LE+D +V   NEKLENIDLS LL+PAA++ P A Q+CV+K
Sbjct: 1321 EIMAQLGFRTVNEMVGRSDMLEIDPKVLEGNEKLENIDLSRLLKPAAEISPGAVQYCVEK 1380

Query: 1381 QDHGLDMALDQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLE 1440
            QDHGLDMALD KLIA S +A+ K + V+IETP+ N+NRAVGTMLSHEVTKRYH+ GLP +
Sbjct: 1381 QDHGLDMALDNKLIASSTAALRKGVRVFIETPVRNINRAVGTMLSHEVTKRYHIHGLPSD 1440

Query: 1441 TIHIKFTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENII 1500
            TIHIK  GSAGQS GAFLCPGI LELEGDSNDYVGKGLSGGKIVVYPP+ S F+P++NI+
Sbjct: 1441 TIHIKLNGSAGQSFGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRNSRFNPQDNIV 1500

Query: 1501 IGNVALYGATSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRN 1560
            IGNVALYGAT GEAYFNGMAAERFCVRNSGA+AVVEG+GDHGCEYMTGGT V+LG TGRN
Sbjct: 1501 IGNVALYGATKGEAYFNGMAAERFCVRNSGAQAVVEGIGDHGCEYMTGGTAVILGKTGRN 1560

Query: 1561 FAAGMSGGVAYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKE 1620
            FAAGMSGG+AYV D+DGKF SRCN ELVDL  V EEDDI TL+MMIQQH+ HT S+LA++
Sbjct: 1561 FAAGMSGGIAYVYDVDGKFSSRCNYELVDLYAVVEEDDITTLRMMIQQHRLHTQSDLARD 1620

Query: 1621 VLENFENLLPRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFE 1680
            +L NF+ LLP+FIKV+PR+YKRVL  +K ++AAKEA + A           E+++K   E
Sbjct: 1621 ILLNFDTLLPKFIKVYPRDYKRVLDKLKEEKAAKEAEQKA----------REVVDKKPVE 1680

Query: 1681 ELKKMAAASLNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKE 1740
             ++  A   ++  +++V   EP  RP+ + +AVK+RGFI YEREG  YRDPN R+ DW E
Sbjct: 1681 VIQ--APNGISVKTEKVMNEEPSSRPSRVSNAVKYRGFIKYEREGTSYRDPNERVKDWNE 1740

Query: 1741 VMEESKPGPLLKTQSARCMDCGTPFCHQENS--GCPLGNKIPEFNELVYQNRWREALERL 1800
            V  E  PGPLLKTQSARCMDCGTPFCHQE S  GCPLGNKIPEFNELV+QNRW EAL+RL
Sbjct: 1741 VAIELVPGPLLKTQSARCMDCGTPFCHQEGSGAGCPLGNKIPEFNELVHQNRWHEALDRL 1800

Query: 1801 LETNNFPEFTGRVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSG 1860
            LETNNFPEFTGRVCPAPCEGSCVLGII+NPVSIK+IECAIIDK FEEGWMVPRPPL R+G
Sbjct: 1801 LETNNFPEFTGRVCPAPCEGSCVLGIIDNPVSIKSIECAIIDKGFEEGWMVPRPPLRRTG 1860

Query: 1861 KRVAIVGSGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNL 1920
            KRVAIVGSGPAGLAAADQLNKMGH VTV+ERADRIGGLMMYGVPNMK DK  +VQRRV L
Sbjct: 1861 KRVAIVGSGPAGLAAADQLNKMGHFVTVFERADRIGGLMMYGVPNMKADKEGIVQRRVEL 1920

Query: 1921 MAEEGVNFVVNANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEF 1980
            MA+EGV F+VNA++G+DP Y++++LR ENDA++LA GATKP+DLP+PGRELAG+HFAMEF
Sbjct: 1921 MAKEGVQFIVNAHVGSDPLYSVEKLRSENDAIILACGATKPKDLPIPGRELAGIHFAMEF 1980

Query: 1981 LHSNTKSLLDSNLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPP 2040
            LH+NTKSLLDSNLE+GNYISA+ +KVVVIGGGDTGTDCIGTSIRHGC+N+VNLELLP+PP
Sbjct: 1981 LHANTKSLLDSNLEDGNYISAQGRKVVVIGGGDTGTDCIGTSIRHGCTNLVNLELLPEPP 2040

Query: 2041 QTRAPGNPWPQWPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQ 2100
            + RAP NPWPQWPRIFRVDYGHQEA +KFGKDPRSY+VLTKRFIGDENG VK LE++RV+
Sbjct: 2041 RKRAPDNPWPQWPRIFRVDYGHQEATSKFGKDPRSYKVLTKRFIGDENGNVKALEVIRVE 2100

Query: 2101 WEKDANGRFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRF 2160
            W K  NGRFQFKEVEGSEEIIEADLVLLAMGFLGPE+TVA KLG+E+D RSNFKA++G F
Sbjct: 2101 WGK-VNGRFQFKEVEGSEEIIEADLVLLAMGFLGPEATVANKLGLEQDMRSNFKAQFGNF 2160

Query: 2161 STNVDGIFAAGDCRRGQSLVVWAINEGRQAAAQVDKYFSKEEK 2193
            +TNV+G+FAAGDCRRGQSLVVWAI EGRQAAA VD Y SK+++
Sbjct: 2161 ATNVEGVFAAGDCRRGQSLVVWAITEGRQAAAAVDNYLSKDDE 2166

BLAST of MS000486 vs. ExPASy Swiss-Prot
Match: Q9C102 (Glutamate synthase [NADH] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=glt1 PE=1 SV=1)

HSP 1 Score: 2299.6 bits (5958), Expect = 0.0e+00
Identity = 1178/2134 (55.20%), Postives = 1512/2134 (70.85%), Query Frame = 0

Query: 92   SGLSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEMLVRMSHRGACGCET 151
            S   A+P K   LYDP+++KDSCGVGF   + G+ S K +TDA  +L  M+HRGA G +T
Sbjct: 48   SWAGALP-KAQALYDPAYEKDSCGVGFTCHIKGQVSHKIVTDARLLLCNMTHRGATGADT 107

Query: 152  NTGDGAGILVALPHEYF-KEARYILCAAKDNGFELPPPGQYAVG-MFFLPTSDSRREESK 211
              GDGAG++  +P+ +  KE   I C        LP  G+YA+G +FF P +D  R E+ 
Sbjct: 108  RDGDGAGVMTGMPYTFMQKEFGQIGCT-------LPKSGEYAIGNVFFSPEADVCR-EAM 167

Query: 212  RVFTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQ--VFLTPSTRSKVD-----L 271
              FT+VAE LG ++L WRSV  DN+ LG +AL  EP I Q  V L  +   + +      
Sbjct: 168  TAFTQVAEKLGLAILAWRSVPCDNSILGPAALSREPTILQPCVVLKAAYDGEAEFDTDLF 227

Query: 272  EKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKDYYLDLGNE 331
            E+Q+Y+LR+ S   I        G  + FYICSL   TIVYKGQL PVQ+ +Y+LDL N 
Sbjct: 228  ERQLYVLRKQSSHLI--------GKEKWFYICSLHRETIVYKGQLAPVQVYNYFLDLNNA 287

Query: 332  RFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLKCQELGL 391
             + S+ AL+HSRFSTNTFPSWDRAQPMR+  HNGEINTLRGN NWM AREGL+K    G 
Sbjct: 288  EYVSHFALVHSRFSTNTFPSWDRAQPMRLAAHNGEINTLRGNKNWMHAREGLMKSSRFG- 347

Query: 392  SEDELKHLLPIVDASSSDSGAFDGVLELLIRAG-RSLPEAVMMMIPEAWQNDKNMDPQRK 451
              +E   LLPI++   SDS AFD V+ELL  +G  SLPEAVM++IPEAWQNDKN+  ++ 
Sbjct: 348  --EEFASLLPIIERGGSDSAAFDNVIELLCASGVVSLPEAVMLLIPEAWQNDKNISDEKA 407

Query: 452  ALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVDI 511
            A YE+ +C MEPWDGPAL +F DGRY GA LDRNGLRP RFY+T    +I ASEVG V I
Sbjct: 408  AFYEWAACQMEPWDGPALFTFADGRYCGANLDRNGLRPCRFYLTSDDMMICASEVGTVGI 467

Query: 512  APEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKDIVGSV 571
             P+ + +KGRL PG MLLVD +   +VDD+ LK   +    +  WL ++ I++  IV S+
Sbjct: 468  EPDRIVQKGRLYPGRMLLVDTKEGRIVDDKELKHNIASRYDFRSWLDQELIDMNSIVDSL 527

Query: 572  DKS---EMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKDGV 631
             +S   ++TP  I   +P + D+              + AFGYT E + M++ PMA  G 
Sbjct: 528  IESTSVDLTP--IVDDVPLADDK-------------TMLAFGYTLEQINMIMAPMANGGK 587

Query: 632  EALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGDLT 691
            E LGSMGND  +A +SD+ +L ++YF+Q+FAQVTNPPIDPIRE IV S+QC IGP G+L 
Sbjct: 588  ETLGSMGNDAAIACLSDQPRLLYDYFRQLFAQVTNPPIDPIREAIVMSLQCYIGPSGNLL 647

Query: 692  ETTEEQCHRLSLKGPLLSIEQMEAIKRMN--YRGWRSKVLDITYPKYLGRRGLEETLDRI 751
            E  + QC RL +  P+L++E+  A+K ++  Y  W+   +DIT+ K  G  G    ++RI
Sbjct: 648  EINQSQCRRLRMPTPILTVEEFNALKNVDRIYPDWKVASIDITFFKSEGVAGYAAAIERI 707

Query: 752  CAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEP 811
            C+EA  A+ EGY  +VLSDR  +++RV ++S+ A GAVH YLV N  R++V L+ ES + 
Sbjct: 708  CSEADTAVNEGYKAIVLSDRNVNSERVPLASIAACGAVHHYLVQNKLRSRVALVCESGDA 767

Query: 812  REVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELHTKEELVKKYFKASNYG 871
            REVHH CTL+G+GADA+CPYLA+EA+ +L     +   +     T+E  +K +  A N G
Sbjct: 768  REVHHMCTLLGYGADAVCPYLAMEALTKL-----VRQNAMKPGITEETAIKNFKHAINGG 827

Query: 872  MMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHE 931
            ++KV++KMGISTL SYKGAQIFEALG+ +EV+ KCF GT SR+ G TFE +A DA  LHE
Sbjct: 828  ILKVMSKMGISTLQSYKGAQIFEALGIDNEVINKCFLGTASRIRGVTFEHIALDAFALHE 887

Query: 932  MAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSK 991
              +PT      S  ++ +P+ GD+++R GGE H+N P AIA LQ+A R  +  AY E+S+
Sbjct: 888  RGYPT----DQSIRSLQIPDMGDFYYRDGGEQHVNHPKAIASLQDAVRNKNEAAYAEFSR 947

Query: 992  LVHELNKACNLRGLLKFK-ETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAM 1051
              +E  + C LRG+L F  ++  +IP+++VEP +EIV+RFCTGAMSYGSIS+E+H++LA+
Sbjct: 948  THYEQTRRCTLRGMLDFDFDSSQAIPIEQVEPWTEIVRRFCTGAMSYGSISMESHSSLAI 1007

Query: 1052 AMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIK 1111
            AMN++GGKSNTGEGGE P+R   L +G  +  RSAIKQ+ASGRFGV+S+YL++ADELQIK
Sbjct: 1008 AMNRLGGKSNTGEGGEDPARSQRLANG--DTMRSAIKQIASGRFGVTSWYLSDADELQIK 1067

Query: 1112 MAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSN 1171
            MAQGAKPGEGGELPG+KV   IA TR+STAGVGLISPPPHHDIYSIEDL QLI+D+K++N
Sbjct: 1068 MAQGAKPGEGGELPGNKVSESIAKTRHSTAGVGLISPPPHHDIYSIEDLKQLIYDMKSAN 1127

Query: 1172 PAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAE 1231
            P AR+SVKLVSE GVG++ASGV K  ADH+L+SGHDGGTGASRWTGIK AGLPWELG+AE
Sbjct: 1128 PRARVSVKLVSEVGVGIVASGVAKAKADHILVSGHDGGTGASRWTGIKYAGLPWELGVAE 1187

Query: 1232 THQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRKCHKN 1291
            THQTLV NDLRGR V+QTDGQ++TGRDVAIA LLGAEE+GF+T PLI +GCIMMRKCH N
Sbjct: 1188 THQTLVLNDLRGRVVIQTDGQIRTGRDVAIACLLGAEEWGFATTPLIALGCIMMRKCHLN 1247

Query: 1292 TCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDLLEVD 1351
            TCPVGIATQDP LR+KF G+PEHV+NFF+ VAEE+R IM++LG RT+N+MVGRSD L+V 
Sbjct: 1248 TCPVGIATQDPELRKKFEGQPEHVVNFFYYVAEELRGIMAKLGFRTINEMVGRSDKLKVA 1307

Query: 1352 KEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMALDQKLIALSKSAIEKS 1411
            + +   N K + +DL+ LL PA  LRP AA + V+KQDH L   LD KLI  ++  +E+ 
Sbjct: 1308 EPI---NNKSKLLDLTPLLTPAFTLRPGAATYNVRKQDHRLYTRLDNKLIDEAEVTLEEG 1367

Query: 1412 LPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAFLCPGIML 1471
            +P  +E  I N +R +G  LS++++KRY   GLP ++I +   GSAGQS GAFL PG+ L
Sbjct: 1368 IPSVVECEIINTDRTLGATLSNKISKRYGEEGLPTDSIRVNVFGSAGQSFGAFLAPGVTL 1427

Query: 1472 ELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFNGMAAERF 1531
            +LEGD NDYVGKGLSGG++++YPP+ S F P+EN+IIGNV LYGATSG A+ +G+AAERF
Sbjct: 1428 QLEGDCNDYVGKGLSGGRLIIYPPRVSPFKPEENMIIGNVCLYGATSGHAFISGVAAERF 1487

Query: 1532 CVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDGKFESRCN 1591
             VRNSGA AVVEGVGDHGCEYMTGG VV+LGSTGRNFAAGMSGG+AYV D+   F  + N
Sbjct: 1488 AVRNSGAIAVVEGVGDHGCEYMTGGRVVILGSTGRNFAAGMSGGIAYVYDMQMDFAGKIN 1547

Query: 1592 LELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFPREYKRVL 1651
             E+VD+  V +  +I  L+ +IQ H+ +T S +A  +L +F   L RF+KV PREYK VL
Sbjct: 1548 TEMVDISSVTDAAEIAFLRGLIQDHRHYTGSQVADRILSDFPRHLSRFVKVLPREYKAVL 1607

Query: 1652 ANMKA-QEAAKEASEPAA--------KEAEEESDEAELIEKDAFEELKKMAAASLNGNSK 1711
                A +E AK    P A        ++ EE + +   +E      +KK A         
Sbjct: 1608 EREAAKKEEAKRLQYPKAFMPGNPIRQQIEETNAQIADVEDTLGATVKKSAPLD------ 1667

Query: 1712 QVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGPLLKTQS 1771
                              K RGF+ Y+R    YR+P  R  DWKE+    +    L+ Q+
Sbjct: 1668 ------------------KLRGFMKYQRRSEHYRNPLKRTNDWKELSVRLREDE-LRVQT 1727

Query: 1772 ARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTGRVCPAP 1831
            ARCMDCGTPFC Q + GCP+ NKI  +N+LV++ +W+EAL +LL TNNFPEFTGRVCPAP
Sbjct: 1728 ARCMDCGTPFC-QSDYGCPISNKIFTWNDLVFKQQWKEALTQLLLTNNFPEFTGRVCPAP 1787

Query: 1832 CEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPAGLAAAD 1891
            CEG+C LGIIE+PV IK++E AIIDKA+EEGW+VPRPP  R+G+RVAI+GSGPAGLAAAD
Sbjct: 1788 CEGACTLGIIESPVGIKSVERAIIDKAWEEGWIVPRPPAERTGRRVAIIGSGPAGLAAAD 1847

Query: 1892 QLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVNANIGTD 1951
            QLN+ GH V +YERADR GGL+ YG+PNMK DK  VV+RR+ LM +EG+  + N  +G +
Sbjct: 1848 QLNRAGHHVVIYERADRPGGLLQYGIPNMKLDK-KVVERRIQLMIDEGIEVLTNVEVGKN 1907

Query: 1952 PSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDSNLENGN 2011
               +LD L    DA+VLA G+T PRDLP+P R+  G+HFAMEFLH NTKSLLDS L++GN
Sbjct: 1908 GDVSLDELHKVYDAVVLASGSTVPRDLPIPNRDSKGIHFAMEFLHKNTKSLLDSELKDGN 1967

Query: 2012 YISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQWPRIFR 2071
            YISAK K V+VIGGGDTG DC+GTS+RHG  +V NLELLP PP+ RA  NPWPQ+PR+FR
Sbjct: 1968 YISAKGKDVIVIGGGDTGNDCLGTSVRHGAKSVRNLELLPIPPRERAFDNPWPQYPRVFR 2027

Query: 2072 VDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQFKEVEGS 2131
            VDYGH E  A +G+D R Y +LTK F  DE+G VKG+  VR++W K++ GR+  KE+  S
Sbjct: 2028 VDYGHAEVQAHYGQDFREYSILTKSFEKDEDGNVKGINTVRIEWTKNSKGRWIMKEIRNS 2087

Query: 2132 EEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAGDCRRGQ 2191
            EE   ADLV+LA+GFLGPE      + V++D RSN       + T+V GI+AAGDCRRGQ
Sbjct: 2088 EEFFPADLVILALGFLGPEEQATAGMNVDRDARSNISTPTKSYETSVPGIYAAGDCRRGQ 2103

Query: 2192 SLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGG 2201
            SLVVW I EGRQ A ++D  F  + K  L G+GG
Sbjct: 2148 SLVVWGIQEGRQCAREIDLKF--QGKTFLPGDGG 2103

BLAST of MS000486 vs. ExPASy TrEMBL
Match: A0A6J1CH78 (glutamate synthase [NADH], amyloplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011404 PE=3 SV=1)

HSP 1 Score: 4434.4 bits (11500), Expect = 0.0e+00
Identity = 2219/2228 (99.60%), Postives = 2221/2228 (99.69%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF
Sbjct: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKE      AAKD
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKE------AAKD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT
Sbjct: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGW+SKVLDITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWQSKVLDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH
Sbjct: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS
Sbjct: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL
Sbjct: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740
            LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP
Sbjct: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740

Query: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800
            LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG
Sbjct: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800

Query: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860
            RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA
Sbjct: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860

Query: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920
            GLAAADQLNKMGH VTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN
Sbjct: 1861 GLAAADQLNKMGHAVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920

Query: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980
            ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS
Sbjct: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980

Query: 1981 NLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040
            NLE+GNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ
Sbjct: 1981 NLEDGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040

Query: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100
            WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF
Sbjct: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100

Query: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160
            KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG
Sbjct: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160

Query: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTS 2220
            DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTS
Sbjct: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTS 2220

Query: 2221 SSRHTVMT 2229
            SSRHTVMT
Sbjct: 2221 SSRHTVMT 2222

BLAST of MS000486 vs. ExPASy TrEMBL
Match: A0A6J1E4X2 (glutamate synthase [NADH], amyloplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430634 PE=3 SV=1)

HSP 1 Score: 4257.2 bits (11040), Expect = 0.0e+00
Identity = 2112/2230 (94.71%), Postives = 2176/2230 (97.58%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSL+KL AAPS+FDK+SVKP LNV  KAR+G RAARCSASK A+RSLNV+EKKFF
Sbjct: 1    MLAKSGSLIKLTAAPSSFDKSSVKPQLNVNSKARIGARAARCSASKAASRSLNVTEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSG +QFWH DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGGVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAG+L+ALPHE+FKE      AA+D
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGLLLALPHEFFKE------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESK+VF KVAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKKVFAKVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            L TEPVIEQVFLTPSTRSKVDLEKQMYILRRLS+VAI+AALNLEHGGARDFYICSLSSRT
Sbjct: 241  LQTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSVVAIQAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKP+QLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPIQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMD QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDAQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK+QKIELKD+VGS+DKSE+T P IAGALPTS D+DNME MGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKRQKIELKDVVGSIDKSELTTPSIAGALPTSADDDNMETMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLPFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPL+SIE+MEAIK+MNYRGWRSKV+DITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLVSIEEMEAIKQMNYRGWRSKVVDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAI+EGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIREGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIPAKS+GE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPAKSNGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVE ASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGTSIPLDEVEAASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM  LPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMESLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMS+LG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSKLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKSLPVYIE+PINNVNRAVGTMLSHEVTKRYHMAGLP ETIHIKF GS
Sbjct: 1381 DQKLIALSKSALEKSLPVYIESPINNVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFNGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGI LELEGDSNDYVGKGLSGGKIVVYPP+ SLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRRSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDI+ LKMMIQQHQRHTSSNLAKEVL+NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDIVALKMMIQQHQRHTSSNLAKEVLDNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKRVLANMKAQ A KE SEPAAK+ EEESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRVLANMKAQGATKEVSEPAAKDVEEESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740
            LNG+SKQVE+TEPPKRPTE  DAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP
Sbjct: 1681 LNGDSKQVEKTEPPKRPTETLDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740

Query: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800
            LLKTQSARCMDCGTPFCHQE+SGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG
Sbjct: 1741 LLKTQSARCMDCGTPFCHQESSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800

Query: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860
            RVCPAPCEGSCVLGIIENPVSIK+IECAIIDKAFEEGWM PRPPLTRSGKRVAI+GSGPA
Sbjct: 1801 RVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMTPRPPLTRSGKRVAIIGSGPA 1860

Query: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920
            GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN
Sbjct: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920

Query: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980
            AN+GTDPSY+LDRL+DENDALVLAVGATKPRDLPVPGREL+GVHFAMEFLHSNTKSLLDS
Sbjct: 1921 ANVGTDPSYSLDRLQDENDALVLAVGATKPRDLPVPGRELSGVHFAMEFLHSNTKSLLDS 1980

Query: 1981 NLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040
            NL++G YISAKDKKVVVIGGGDTGTDCIGTSIRHGCS+VVNLELLPQPPQTRAPGNPWPQ
Sbjct: 1981 NLQDGKYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSVVNLELLPQPPQTRAPGNPWPQ 2040

Query: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100
            WPRIFRVDYGHQEAA KFGKDPRSYEVLTKRFIGDENG VKGLE++RVQWEKDANGRFQF
Sbjct: 2041 WPRIFRVDYGHQEAATKFGKDPRSYEVLTKRFIGDENGVVKGLEVIRVQWEKDANGRFQF 2100

Query: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160
            KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFST+VDGIFAAG
Sbjct: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTSVDGIFAAG 2160

Query: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQD--S 2220
            DCRRGQSLVVWAI+EGRQAAAQVDKY SKEEKN+LVGEGGYEGVG  SQDYNNRQQD  S
Sbjct: 2161 DCRRGQSLVVWAISEGRQAAAQVDKYLSKEEKNLLVGEGGYEGVGKSSQDYNNRQQDSSS 2220

Query: 2221 TSSSRHTVMT 2229
            +SSSRHT+MT
Sbjct: 2221 SSSSRHTIMT 2224

BLAST of MS000486 vs. ExPASy TrEMBL
Match: A0A6J1E9L3 (glutamate synthase [NADH], amyloplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430634 PE=3 SV=1)

HSP 1 Score: 4251.4 bits (11025), Expect = 0.0e+00
Identity = 2112/2234 (94.54%), Postives = 2176/2234 (97.40%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSL+KL AAPS+FDK+SVKP LNV  KAR+G RAARCSASK A+RSLNV+EKKFF
Sbjct: 1    MLAKSGSLIKLTAAPSSFDKSSVKPQLNVNSKARIGARAARCSASKAASRSLNVTEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSG +QFWH DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGGVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAG+L+ALPHE+FKE      AA+D
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGLLLALPHEFFKE------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESK+VF KVAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKKVFAKVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            L TEPVIEQVFLTPSTRSKVDLEKQMYILRRLS+VAI+AALNLEHGGARDFYICSLSSRT
Sbjct: 241  LQTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSVVAIQAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKP+QLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPIQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMD QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDAQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK+QKIELKD+VGS+DKSE+T P IAGALPTS D+DNME MGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKRQKIELKDVVGSIDKSELTTPSIAGALPTSADDDNMETMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLPFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPL+SIE+MEAIK+MNYRGWRSKV+DITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLVSIEEMEAIKQMNYRGWRSKVVDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAI+EGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIREGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIPAKS+GE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPAKSNGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVE ASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGTSIPLDEVEAASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM  LPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMESLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMS+LG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSKLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKSLPVYIE+PINNVNRAVGTMLSHEVTKRYHMAGLP ETIHIKF GS
Sbjct: 1381 DQKLIALSKSALEKSLPVYIESPINNVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFNGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGI LELEGDSNDYVGKGLSGGKIVVYPP+ SLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGITLELEGDSNDYVGKGLSGGKIVVYPPRRSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDI+ LKMMIQQHQRHTSSNLAKEVL+NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDIVALKMMIQQHQRHTSSNLAKEVLDNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKRVLANMKAQ A KE SEPAAK+ EEESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRVLANMKAQGATKEVSEPAAKDVEEESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQ----VEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES 1740
            LNG+SKQ    VE+TEPPKRPTE  DAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES
Sbjct: 1681 LNGDSKQLDPKVEKTEPPKRPTETLDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEES 1740

Query: 1741 KPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFP 1800
            KPGPLLKTQSARCMDCGTPFCHQE+SGCPLGNKIPEFNELVYQNRWREALERLLETNNFP
Sbjct: 1741 KPGPLLKTQSARCMDCGTPFCHQESSGCPLGNKIPEFNELVYQNRWREALERLLETNNFP 1800

Query: 1801 EFTGRVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVG 1860
            EFTGRVCPAPCEGSCVLGIIENPVSIK+IECAIIDKAFEEGWM PRPPLTRSGKRVAI+G
Sbjct: 1801 EFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMTPRPPLTRSGKRVAIIG 1860

Query: 1861 SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN 1920
            SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN
Sbjct: 1861 SGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVN 1920

Query: 1921 FVVNANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKS 1980
            FVVNAN+GTDPSY+LDRL+DENDALVLAVGATKPRDLPVPGREL+GVHFAMEFLHSNTKS
Sbjct: 1921 FVVNANVGTDPSYSLDRLQDENDALVLAVGATKPRDLPVPGRELSGVHFAMEFLHSNTKS 1980

Query: 1981 LLDSNLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGN 2040
            LLDSNL++G YISAKDKKVVVIGGGDTGTDCIGTSIRHGCS+VVNLELLPQPPQTRAPGN
Sbjct: 1981 LLDSNLQDGKYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSVVNLELLPQPPQTRAPGN 2040

Query: 2041 PWPQWPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANG 2100
            PWPQWPRIFRVDYGHQEAA KFGKDPRSYEVLTKRFIGDENG VKGLE++RVQWEKDANG
Sbjct: 2041 PWPQWPRIFRVDYGHQEAATKFGKDPRSYEVLTKRFIGDENGVVKGLEVIRVQWEKDANG 2100

Query: 2101 RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGI 2160
            RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFST+VDGI
Sbjct: 2101 RFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTSVDGI 2160

Query: 2161 FAAGDCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQ 2220
            FAAGDCRRGQSLVVWAI+EGRQAAAQVDKY SKEEKN+LVGEGGYEGVG  SQDYNNRQQ
Sbjct: 2161 FAAGDCRRGQSLVVWAISEGRQAAAQVDKYLSKEEKNLLVGEGGYEGVGKSSQDYNNRQQ 2220

Query: 2221 D--STSSSRHTVMT 2229
            D  S+SSSRHT+MT
Sbjct: 2221 DSSSSSSSRHTIMT 2228

BLAST of MS000486 vs. ExPASy TrEMBL
Match: A0A6J1J2E0 (glutamate synthase [NADH], amyloplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482791 PE=3 SV=1)

HSP 1 Score: 4242.6 bits (11002), Expect = 0.0e+00
Identity = 2110/2237 (94.32%), Postives = 2174/2237 (97.18%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAKSGSLLKL A PS+FDK+SVKP LNV  KAR+G RAARCSASK A+RSLN+SEKKFF
Sbjct: 1    MLAKSGSLLKLTATPSSFDKSSVKPQLNVNSKARIGARAARCSASKAASRSLNLSEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSGR+QFWH DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGRVQFWHLDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAG+L+ALPHE+FKE      AA+D
Sbjct: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGLLLALPHEFFKE------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPPPGQYAVGMFFLPTSDSRREESK+VF KVAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKKVFAKVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            L TEPVIEQVFLTPSTRSKVDLEKQMYILRRLS+VAI+AAL+LEHGGARDFYICSLSSRT
Sbjct: 241  LQTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSVVAIQAALSLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKP+QLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPIQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMD QRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDAQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK+QKIELKD+VGS+DKSE+T P IAGALPTS D+DNME MGIHGLLAPLKAFG
Sbjct: 541  RPYGEWLKRQKIELKDVVGSIDKSELTTPSIAGALPTSADDDNMETMGIHGLLAPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLPFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPL+SIE+MEAIK+MNYRGWRSKV+DITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLVSIEEMEAIKQMNYRGWRSKVVDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRICAEAHDAI+EGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICAEAHDAIREGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQVDGKIPAKS+GE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQVDGKIPAKSNGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVV+KCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVDKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVAIAKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAIAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVE ASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGTSIPLDEVEAASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM  LPDGSMNPKRSAIKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMESLPDGSMNPKRSAIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMS+LG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSKLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKSLPVYIE+PINNVNRAVGTMLSHEVTKRYHMAGLP ETIHIKF GS
Sbjct: 1381 DQKLIALSKSALEKSLPVYIESPINNVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFNGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPP+ SLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPRRSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDI+ LKMMIQQHQRHTSSNLAKEVL NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDIVALKMMIQQHQRHTSSNLAKEVLNNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKRVLANMK+Q A KE SEPAAK+  EESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRVLANMKSQGATKEVSEPAAKDV-EESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQ--------VEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEV 1740
            LNG+SKQ        VE+ EPPKRPTE PDAVKHRGFIAYEREGVKYRDPNVRMGDWKEV
Sbjct: 1681 LNGDSKQVNFEFNPKVEKMEPPKRPTETPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEV 1740

Query: 1741 MEESKPGPLLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLET 1800
            MEESKPGPLLKTQSARCMDCGTPFCHQE+SGCPLGNKIPEFNELVYQNRWREALERLLET
Sbjct: 1741 MEESKPGPLLKTQSARCMDCGTPFCHQESSGCPLGNKIPEFNELVYQNRWREALERLLET 1800

Query: 1801 NNFPEFTGRVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRV 1860
            NNFPEFTGRVCPAPCEGSCVLGIIENPVSIK+IECAIIDKAFEEGWM PRPPLTRSGKRV
Sbjct: 1801 NNFPEFTGRVCPAPCEGSCVLGIIENPVSIKSIECAIIDKAFEEGWMTPRPPLTRSGKRV 1860

Query: 1861 AIVGSGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAE 1920
            AIVGSGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAE
Sbjct: 1861 AIVGSGPAGLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAE 1920

Query: 1921 EGVNFVVNANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHS 1980
            EGVNFVVNAN+GTDPSY+LDRL+DENDALVLAVGATKPRDLPVPGREL+GVHFAMEFLHS
Sbjct: 1921 EGVNFVVNANVGTDPSYSLDRLQDENDALVLAVGATKPRDLPVPGRELSGVHFAMEFLHS 1980

Query: 1981 NTKSLLDSNLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTR 2040
            NTKSLLDSNL++G YISAKDKKVVVIGGGDTGTDCIGTSIRHGCS+VVNLELLPQPPQTR
Sbjct: 1981 NTKSLLDSNLQDGKYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSVVNLELLPQPPQTR 2040

Query: 2041 APGNPWPQWPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEK 2100
            A GNPWPQWPRIFRVDYGHQEAA KFGKDPRSYEVLTKRFIGDENG VKGLE++RVQWEK
Sbjct: 2041 AAGNPWPQWPRIFRVDYGHQEAATKFGKDPRSYEVLTKRFIGDENGVVKGLEVIRVQWEK 2100

Query: 2101 DANGRFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTN 2160
            DANGRFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFST+
Sbjct: 2101 DANGRFQFKEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTS 2160

Query: 2161 VDGIFAAGDCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYN 2220
            VDGIFAAGDCRRGQSLVVWAI+EGRQAAAQVDKY SKEEKN+LVGEGGYEGVG  SQDYN
Sbjct: 2161 VDGIFAAGDCRRGQSLVVWAISEGRQAAAQVDKYLSKEEKNLLVGEGGYEGVGKSSQDYN 2220

Query: 2221 NRQQD-STSSSRHTVMT 2229
            NRQQD S+SSSRHT+MT
Sbjct: 2221 NRQQDSSSSSSRHTIMT 2230

BLAST of MS000486 vs. ExPASy TrEMBL
Match: A0A5A7UAB6 (Glutamate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00950 PE=3 SV=1)

HSP 1 Score: 4240.3 bits (10996), Expect = 0.0e+00
Identity = 2102/2228 (94.34%), Postives = 2169/2228 (97.35%), Query Frame = 0

Query: 1    MLAKSGSLLKLPAAPSTFDKTSVKPHLNVTPKARLGTRAARCSASKGATRSLNVSEKKFF 60
            MLAK GSLLKLPAAP +FD TSVKP LNV PKARLG RAARCSASKG +  LNVSEKKFF
Sbjct: 1    MLAKPGSLLKLPAAPYSFDNTSVKPQLNVNPKARLGARAARCSASKGGSGLLNVSEKKFF 60

Query: 61   GARLRAPGSGRIQFWHSDGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120
            GARLRAPGSGR QFWH DGPGRSPKLRL +RSGLSAVPEKPLGLYDPSFDKDSCGVGFVA
Sbjct: 61   GARLRAPGSGRFQFWHLDGPGRSPKLRLAIRSGLSAVPEKPLGLYDPSFDKDSCGVGFVA 120

Query: 121  ELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKD 180
            ELSGE+SRKTITDALEMLVRMSHRGACGCETNTGDGAGIL+ALPHE+FK+      AA+D
Sbjct: 121  ELSGETSRKTITDALEMLVRMSHRGACGCETNTGDGAGILLALPHEFFKQ------AARD 180

Query: 181  NGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSA 240
            NGFELPP GQYAVGMFFLPTSDSRREESK+VF +VAESLGHSVLGWRSV+TDNTGLGKSA
Sbjct: 181  NGFELPPAGQYAVGMFFLPTSDSRREESKKVFAQVAESLGHSVLGWRSVQTDNTGLGKSA 240

Query: 241  LLTEPVIEQVFLTPSTRSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300
            LLTEPVIEQVFLTPST+SKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT
Sbjct: 241  LLTEPVIEQVFLTPSTKSKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRT 300

Query: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360
            IVYKGQLKPVQLKDYYLDLGNERFTSYMAL+HSRFSTNTFPSWDRAQPMRVLGHNGEINT
Sbjct: 301  IVYKGQLKPVQLKDYYLDLGNERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINT 360

Query: 361  LRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420
            LRGNVNWMKAREGLLKC+ELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE
Sbjct: 361  LRGNVNWMKAREGLLKCKELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPE 420

Query: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480
            AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG
Sbjct: 421  AVMMMIPEAWQNDKNMDPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPG 480

Query: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540
            RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA
Sbjct: 481  RFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLA 540

Query: 541  RPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFG 600
            RPYGEWLK QKIELKD++GS+DKSEMT P I GAL   + EDNM NMGIHGLL PLKAFG
Sbjct: 541  RPYGEWLKNQKIELKDVIGSIDKSEMTTPAITGAL---SAEDNMNNMGIHGLLTPLKAFG 600

Query: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIR 660
            YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMS+REKLTFEYFKQMFAQVTNPPIDPIR
Sbjct: 601  YTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSNREKLTFEYFKQMFAQVTNPPIDPIR 660

Query: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITY 720
            EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSI +MEAIK+MNYRGWRSKVLDITY
Sbjct: 661  EKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIGEMEAIKKMNYRGWRSKVLDITY 720

Query: 721  PKYLGRRGLEETLDRICAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVN 780
            PKYLGRRGLEETLDRIC+EA +AI+EG+TTLVLSDRAFS+KRVAVSSLLAVGAVHQYLV 
Sbjct: 721  PKYLGRRGLEETLDRICSEAQNAIQEGFTTLVLSDRAFSSKRVAVSSLLAVGAVHQYLVK 780

Query: 781  NLERTQVGLIIESAEPREVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELH 840
            NLERTQVGLI+ESAEPREVHHFCTLVGFGADAICPYLA+EAIWRLQ+DGKIPAKSSGE H
Sbjct: 781  NLERTQVGLIVESAEPREVHHFCTLVGFGADAICPYLAIEAIWRLQIDGKIPAKSSGEFH 840

Query: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900
            TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE
Sbjct: 841  TKEELVKKYFKASNYGMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVE 900

Query: 901  GATFEMLARDALNLHEMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQ 960
            GATFEMLARDA NLHEMAFP+R FP GSAEAVALPNPGDYHWRKGGE+HLNDPVA+AKLQ
Sbjct: 901  GATFEMLARDAHNLHEMAFPSRAFPPGSAEAVALPNPGDYHWRKGGEIHLNDPVAMAKLQ 960

Query: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAM 1020
            EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETG SIPLDEVEPASEIVKRFCTGAM
Sbjct: 961  EAARTNSVNAYKEYSKLVHELNKACNLRGLLKFKETGASIPLDEVEPASEIVKRFCTGAM 1020

Query: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFG 1080
            SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRM PLPDGSMNPKRS+IKQVASGRFG
Sbjct: 1021 SYGSISLEAHTTLAMAMNKIGGKSNTGEGGEQPSRMEPLPDGSMNPKRSSIKQVASGRFG 1080

Query: 1081 VSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYS 1140
            VS YYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIA TRNSTAGVGLISPPPHHDIYS
Sbjct: 1081 VSMYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIAKTRNSTAGVGLISPPPHHDIYS 1140

Query: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200
            IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT
Sbjct: 1141 IEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWT 1200

Query: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260
            GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP
Sbjct: 1201 GIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAP 1260

Query: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVR 1320
            LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLG R
Sbjct: 1261 LITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGFR 1320

Query: 1321 TVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMAL 1380
            TVNQMVGRSD+LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQ+CVQKQDHGLDMAL
Sbjct: 1321 TVNQMVGRSDVLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQYCVQKQDHGLDMAL 1380

Query: 1381 DQKLIALSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGS 1440
            DQKLIALSKSA+EKS+PVYIETPI NVNRAVGTMLSHEVTKRYHMAGLP ETIHIKFTGS
Sbjct: 1381 DQKLIALSKSALEKSIPVYIETPIINVNRAVGTMLSHEVTKRYHMAGLPSETIHIKFTGS 1440

Query: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500
            AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA
Sbjct: 1441 AGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGA 1500

Query: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGV 1560
            TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVV+LG TGRNFAAGMSGG+
Sbjct: 1501 TSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVILGKTGRNFAAGMSGGI 1560

Query: 1561 AYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLL 1620
            AYVLD+DGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVL+NFENLL
Sbjct: 1561 AYVLDMDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLDNFENLL 1620

Query: 1621 PRFIKVFPREYKRVLANMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS 1680
            PRFIKVFPREYKR+LA+MKAQEA KEA EP+AK+A EESDEAEL+EKDAFEELKKMAAAS
Sbjct: 1621 PRFIKVFPREYKRILADMKAQEAVKEALEPSAKDA-EESDEAELVEKDAFEELKKMAAAS 1680

Query: 1681 LNGNSKQVEETEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGP 1740
            LNGNS+QVE+TEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRM DWKEVMEESKPGP
Sbjct: 1681 LNGNSEQVEKTEPPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMRDWKEVMEESKPGP 1740

Query: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800
            LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG
Sbjct: 1741 LLKTQSARCMDCGTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTG 1800

Query: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPA 1860
            RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWM+PRPP  RSGKRVAIVGSGPA
Sbjct: 1801 RVCPAPCEGSCVLGIIENPVSIKNIECAIIDKAFEEGWMIPRPPQVRSGKRVAIVGSGPA 1860

Query: 1861 GLAAADQLNKMGHTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920
            GLAAADQLNKMGH VTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN
Sbjct: 1861 GLAAADQLNKMGHKVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVN 1920

Query: 1921 ANIGTDPSYTLDRLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980
            AN+GTD SY+LD+LR ENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS
Sbjct: 1921 ANVGTDTSYSLDQLRKENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDS 1980

Query: 1981 NLENGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQ 2040
            NL++GNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCS++VNLELLPQPPQTRAPGNPWPQ
Sbjct: 1981 NLQDGNYISAKDKKVVVIGGGDTGTDCIGTSIRHGCSSIVNLELLPQPPQTRAPGNPWPQ 2040

Query: 2041 WPRIFRVDYGHQEAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQF 2100
            WPRIFRVDYGHQEAAAKFGKDPR+YEVLTKRFIGDENG VKGLE++RVQWEKDA+GRFQF
Sbjct: 2041 WPRIFRVDYGHQEAAAKFGKDPRTYEVLTKRFIGDENGVVKGLEVIRVQWEKDADGRFQF 2100

Query: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAG 2160
            KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLG+EKDNRSNFKAEYGRFST+VDG+FAAG
Sbjct: 2101 KEVEGSEEIIEADLVLLAMGFLGPESTVAEKLGIEKDNRSNFKAEYGRFSTSVDGVFAAG 2160

Query: 2161 DCRRGQSLVVWAINEGRQAAAQVDKYFSKEEKNILVGEGGYEGVGNRSQDYNNRQQDSTS 2220
            DCRRGQSLVVWAI+EGRQAAAQVDKY +KEEK  +VGEGGYEGVGN SQDYNNRQQDS+S
Sbjct: 2161 DCRRGQSLVVWAISEGRQAAAQVDKYLAKEEKGGIVGEGGYEGVGNGSQDYNNRQQDSSS 2218

Query: 2221 SSRHTVMT 2229
            SSRHTVMT
Sbjct: 2221 SSRHTVMT 2218

BLAST of MS000486 vs. TAIR 10
Match: AT5G53460.1 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 3702.5 bits (9600), Expect = 0.0e+00
Identity = 1822/2179 (83.62%), Postives = 1988/2179 (91.23%), Query Frame = 0

Query: 19   DKTSVKPHLNVTP-KARLGTRAARCSASKGATRSLNVSEKKFFGARLRAPGSGRIQFWHS 78
            + TSV   L VT   +R  +  ARCS  K       + E  F G R+R  GS  +QFW S
Sbjct: 26   NSTSVASQLAVTSGVSRRRSCTARCSVKKPV-----IPESPFLGTRVRRSGSETLQFWRS 85

Query: 79   DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEM 138
            DGPGRS KLR VV+S  SAVPEKPLGLYDPS+DKDSCGVGFVAELSGE++RKT+TD+LEM
Sbjct: 86   DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEM 145

Query: 139  LVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKDNGFELPPPGQYAVGMFF 198
            L+RM+HRGACGCE+NTGDGAGILV LPH+++ E      AA + GF LP  G YAVGMFF
Sbjct: 146  LIRMTHRGACGCESNTGDGAGILVGLPHDFYAE------AATELGFVLPSAGNYAVGMFF 205

Query: 199  LPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTR 258
            LPT +SRREESK VFTKVAESLGHSVLGWR V TDN+GLG SAL TEP+I QVFLTP+T+
Sbjct: 206  LPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTK 265

Query: 259  SKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKD-YY 318
            SK D E+QMYILRR+SMVAIRAALNL+HG  +DFYICSLSSRTIVYKGQLKP QLKD YY
Sbjct: 266  SKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYY 325

Query: 319  LDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLK 378
             DLG+ERFTSYMAL+HSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWM+AREGLLK
Sbjct: 326  ADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLK 385

Query: 379  CQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNM 438
            C ELGLS+ ELK LLPIVD SSSDSGAFDGVLELL+RAGRSLPEAVMMMIPEAWQNDKN+
Sbjct: 386  CNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNI 445

Query: 439  DPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEV 498
            DP RK  YEY S LMEPWDGPALISFTDGRYLGATLDRNGLRPGRFY+THSGRVIMASEV
Sbjct: 446  DPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEV 505

Query: 499  GVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKD 558
            GVVD+ PEDV RKGRLNPGMMLLVDFE H+VVDD+ALKQQYSLARPYGEWLK+QKIELKD
Sbjct: 506  GVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKD 565

Query: 559  IVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKD 618
            I+ SV ++E   P I+G +P S D+D+ME+MGIHGLL+PLKAFGYT EALEMLLLPMAKD
Sbjct: 566  IIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKD 625

Query: 619  GVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGD 678
            G EALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIREKIVTSM+CMIGPEGD
Sbjct: 626  GSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGD 685

Query: 679  LTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITYPKYLGRRGLEETLDRI 738
            LTETTEEQCHRLSLKGPLL IE+MEAIK+MNYRGWR+KVLDITY K  G +GLEETLDRI
Sbjct: 686  LTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRI 745

Query: 739  CAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEP 798
            C EA++AIKEGYT LVLSDRAFS  RVAVSSL+AVGAVH +LV  L RTQVGL++ESAEP
Sbjct: 746  CDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEP 805

Query: 799  REVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELHTKEELVKKYFKASNYG 858
            REVHHFCTLVGFGADAICPYLAVEA++RLQVDGKIP KS+GE H+KEELVKKY+KASNYG
Sbjct: 806  REVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYG 865

Query: 859  MMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHE 918
            MMKVLAKMGISTLASYKGAQIFEALGLSSEV++KCFAGTPSRVEGATFEMLARD L LHE
Sbjct: 866  MMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHE 925

Query: 919  MAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSK 978
            +AFPTR +  GSAEA AL NPG+YHWRK GE+HLNDP+AIAKLQEAARTNSV AYKEYSK
Sbjct: 926  LAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSK 985

Query: 979  LVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1038
             ++ELNK  NLRGL+KFK+  V IPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA
Sbjct: 986  RINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1045

Query: 1039 MNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKM 1098
            MNK+GGKSNTGEGGE PSRM PL DGS NPKRS+IKQ+ASGRFGVSSYYLTNADELQIKM
Sbjct: 1046 MNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKM 1105

Query: 1099 AQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNP 1158
            AQGAKPGEGGELPGHKV+G+IA TRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN+NP
Sbjct: 1106 AQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANP 1165

Query: 1159 AARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAET 1218
             ARISVKLVSEAGVGVIASGVVKGHADHVLI+GHDGGTGASRWTGIKNAGLPWELGLAET
Sbjct: 1166 GARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAET 1225

Query: 1219 HQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRKCHKNT 1278
            HQTLVANDLRGRTVLQTDGQLKTGRDVA+AALLGAEEFGFSTAPLIT+GCIMMRKCHKNT
Sbjct: 1226 HQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNT 1285

Query: 1279 CPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDLLEVDK 1338
            CPVGIATQDPVLREKFAGEPEHVINFFFM+AEE+REIMS LG RTV +M+GR+D+LE+D+
Sbjct: 1286 CPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDR 1345

Query: 1339 EVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMALDQKLIALSKSAIEKSL 1398
            EV   N+KLENIDLSLLLRPAA++RP AAQ+CVQKQDHGLDMALDQ+LIALSKSA+EKSL
Sbjct: 1346 EVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSL 1405

Query: 1399 PVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAFLCPGIMLE 1458
            PVYIETPI NVNRAVGTMLSHEVTKRYH+ GLP +TIHIKFTGSAGQSLGAFLCPGIMLE
Sbjct: 1406 PVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLE 1465

Query: 1459 LEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFNGMAAERFC 1518
            LEGDSNDYVGKGLSGGK+VVYPPKGS FDPKENI+IGNVALYGATSGEAYFNGMAAERF 
Sbjct: 1466 LEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFS 1525

Query: 1519 VRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDGKFESRCNL 1578
            VRNSGAKAVVEG+GDHGCEYMTGGTVVVLG TGRNFAAGMSGG+AYVLD+DGKF +RCNL
Sbjct: 1526 VRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNL 1585

Query: 1579 ELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFPREYKRVLA 1638
            ELVDLDKVE+E+D +TLKMMIQQHQRHT+S LA+EVL +FENLLP+FIKVFPR+YKRVL+
Sbjct: 1586 ELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLS 1645

Query: 1639 NMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS----LNGNSKQVEETE 1698
             MK +E +K+A E A++EA +E++E EL EKDAF ELK MAAAS    ++GN    E   
Sbjct: 1646 AMKHEEVSKQAIERASEEA-DETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAE--- 1705

Query: 1699 PPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGPLLKTQSARCMDC 1758
               RP+++ +AVK+ GFIAYEREGVKYRDPNVR+ DW EVMEESKPGPLL TQSARCMDC
Sbjct: 1706 --ARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDC 1765

Query: 1759 GTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTGRVCPAPCEGSCV 1818
            GTPFCHQENSGCPLGNKIPEFNELVYQNRW+EAL RLLETNNFPEFTGRVCPAPCEGSCV
Sbjct: 1766 GTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCV 1825

Query: 1819 LGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPAGLAAADQLNKMG 1878
            LGIIENPVSIK+IECAIIDKAFEEGWMVPRPPL R+GK+VAI+GSGPAGLAAADQLNKMG
Sbjct: 1826 LGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMG 1885

Query: 1879 HTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVNANIGTDPSYTLD 1938
            H VTVYER+DRIGGLMMYGVPNMKTDK+DVVQRRV+LM +EG+NFVVNANIG DPSY+LD
Sbjct: 1886 HLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLD 1945

Query: 1939 RLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDSNLENGNYISAKD 1998
             L++ENDA+VLAVG+TKPRDLPVPGR+L+GVHFAMEFLH+NTKSLLDSN E+GNYISAK 
Sbjct: 1946 GLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKG 2005

Query: 1999 KKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQWPRIFRVDYGHQ 2058
            KKVVVIGGGDTGTDCIGTSIRHGC+N+VNLELLPQPP TRAPGNPWPQWPR+FR+DYGHQ
Sbjct: 2006 KKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQ 2065

Query: 2059 EAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQFKEVEGSEEIIEA 2118
            EA  KFGKDPR+YEVLTKRFIGD+NG VKGLELVRV WEKD  GRFQFKE+EGSEEIIEA
Sbjct: 2066 EATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEA 2125

Query: 2119 DLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAGDCRRGQSLVVWA 2178
            DLV LAMGFLGPE T+AEKLG+E DNRSNFKAEYGRFST V+G+FAAGDCRRGQSLVVWA
Sbjct: 2126 DLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWA 2185

Query: 2179 INEGRQAAAQVDKYFSKEE 2192
            I+EGRQAA QVDK+ +K +
Sbjct: 2186 ISEGRQAADQVDKFLTKTD 2187

BLAST of MS000486 vs. TAIR 10
Match: AT5G53460.2 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 3702.5 bits (9600), Expect = 0.0e+00
Identity = 1822/2179 (83.62%), Postives = 1988/2179 (91.23%), Query Frame = 0

Query: 19   DKTSVKPHLNVTP-KARLGTRAARCSASKGATRSLNVSEKKFFGARLRAPGSGRIQFWHS 78
            + TSV   L VT   +R  +  ARCS  K       + E  F G R+R  GS  +QFW S
Sbjct: 26   NSTSVASQLAVTSGVSRRRSCTARCSVKKPV-----IPESPFLGTRVRRSGSETLQFWRS 85

Query: 79   DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEM 138
            DGPGRS KLR VV+S  SAVPEKPLGLYDPS+DKDSCGVGFVAELSGE++RKT+TD+LEM
Sbjct: 86   DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEM 145

Query: 139  LVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKDNGFELPPPGQYAVGMFF 198
            L+RM+HRGACGCE+NTGDGAGILV LPH+++ E      AA + GF LP  G YAVGMFF
Sbjct: 146  LIRMTHRGACGCESNTGDGAGILVGLPHDFYAE------AATELGFVLPSAGNYAVGMFF 205

Query: 199  LPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTR 258
            LPT +SRREESK VFTKVAESLGHSVLGWR V TDN+GLG SAL TEP+I QVFLTP+T+
Sbjct: 206  LPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTK 265

Query: 259  SKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKD-YY 318
            SK D E+QMYILRR+SMVAIRAALNL+HG  +DFYICSLSSRTIVYKGQLKP QLKD YY
Sbjct: 266  SKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYY 325

Query: 319  LDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLK 378
             DLG+ERFTSYMAL+HSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWM+AREGLLK
Sbjct: 326  ADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLK 385

Query: 379  CQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNM 438
            C ELGLS+ ELK LLPIVD SSSDSGAFDGVLELL+RAGRSLPEAVMMMIPEAWQNDKN+
Sbjct: 386  CNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNI 445

Query: 439  DPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEV 498
            DP RK  YEY S LMEPWDGPALISFTDGRYLGATLDRNGLRPGRFY+THSGRVIMASEV
Sbjct: 446  DPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEV 505

Query: 499  GVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKD 558
            GVVD+ PEDV RKGRLNPGMMLLVDFE H+VVDD+ALKQQYSLARPYGEWLK+QKIELKD
Sbjct: 506  GVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKD 565

Query: 559  IVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKD 618
            I+ SV ++E   P I+G +P S D+D+ME+MGIHGLL+PLKAFGYT EALEMLLLPMAKD
Sbjct: 566  IIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKD 625

Query: 619  GVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGD 678
            G EALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIREKIVTSM+CMIGPEGD
Sbjct: 626  GSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGD 685

Query: 679  LTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITYPKYLGRRGLEETLDRI 738
            LTETTEEQCHRLSLKGPLL IE+MEAIK+MNYRGWR+KVLDITY K  G +GLEETLDRI
Sbjct: 686  LTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRI 745

Query: 739  CAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEP 798
            C EA++AIKEGYT LVLSDRAFS  RVAVSSL+AVGAVH +LV  L RTQVGL++ESAEP
Sbjct: 746  CDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEP 805

Query: 799  REVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELHTKEELVKKYFKASNYG 858
            REVHHFCTLVGFGADAICPYLAVEA++RLQVDGKIP KS+GE H+KEELVKKY+KASNYG
Sbjct: 806  REVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYG 865

Query: 859  MMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHE 918
            MMKVLAKMGISTLASYKGAQIFEALGLSSEV++KCFAGTPSRVEGATFEMLARD L LHE
Sbjct: 866  MMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHE 925

Query: 919  MAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSK 978
            +AFPTR +  GSAEA AL NPG+YHWRK GE+HLNDP+AIAKLQEAARTNSV AYKEYSK
Sbjct: 926  LAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSK 985

Query: 979  LVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1038
             ++ELNK  NLRGL+KFK+  V IPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA
Sbjct: 986  RINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1045

Query: 1039 MNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKM 1098
            MNK+GGKSNTGEGGE PSRM PL DGS NPKRS+IKQ+ASGRFGVSSYYLTNADELQIKM
Sbjct: 1046 MNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKM 1105

Query: 1099 AQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNP 1158
            AQGAKPGEGGELPGHKV+G+IA TRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN+NP
Sbjct: 1106 AQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANP 1165

Query: 1159 AARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAET 1218
             ARISVKLVSEAGVGVIASGVVKGHADHVLI+GHDGGTGASRWTGIKNAGLPWELGLAET
Sbjct: 1166 GARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAET 1225

Query: 1219 HQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRKCHKNT 1278
            HQTLVANDLRGRTVLQTDGQLKTGRDVA+AALLGAEEFGFSTAPLIT+GCIMMRKCHKNT
Sbjct: 1226 HQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNT 1285

Query: 1279 CPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDLLEVDK 1338
            CPVGIATQDPVLREKFAGEPEHVINFFFM+AEE+REIMS LG RTV +M+GR+D+LE+D+
Sbjct: 1286 CPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDR 1345

Query: 1339 EVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMALDQKLIALSKSAIEKSL 1398
            EV   N+KLENIDLSLLLRPAA++RP AAQ+CVQKQDHGLDMALDQ+LIALSKSA+EKSL
Sbjct: 1346 EVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSL 1405

Query: 1399 PVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAFLCPGIMLE 1458
            PVYIETPI NVNRAVGTMLSHEVTKRYH+ GLP +TIHIKFTGSAGQSLGAFLCPGIMLE
Sbjct: 1406 PVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLE 1465

Query: 1459 LEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFNGMAAERFC 1518
            LEGDSNDYVGKGLSGGK+VVYPPKGS FDPKENI+IGNVALYGATSGEAYFNGMAAERF 
Sbjct: 1466 LEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFS 1525

Query: 1519 VRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDGKFESRCNL 1578
            VRNSGAKAVVEG+GDHGCEYMTGGTVVVLG TGRNFAAGMSGG+AYVLD+DGKF +RCNL
Sbjct: 1526 VRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNL 1585

Query: 1579 ELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFPREYKRVLA 1638
            ELVDLDKVE+E+D +TLKMMIQQHQRHT+S LA+EVL +FENLLP+FIKVFPR+YKRVL+
Sbjct: 1586 ELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLS 1645

Query: 1639 NMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS----LNGNSKQVEETE 1698
             MK +E +K+A E A++EA +E++E EL EKDAF ELK MAAAS    ++GN    E   
Sbjct: 1646 AMKHEEVSKQAIERASEEA-DETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAE--- 1705

Query: 1699 PPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGPLLKTQSARCMDC 1758
               RP+++ +AVK+ GFIAYEREGVKYRDPNVR+ DW EVMEESKPGPLL TQSARCMDC
Sbjct: 1706 --ARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDC 1765

Query: 1759 GTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTGRVCPAPCEGSCV 1818
            GTPFCHQENSGCPLGNKIPEFNELVYQNRW+EAL RLLETNNFPEFTGRVCPAPCEGSCV
Sbjct: 1766 GTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCV 1825

Query: 1819 LGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPAGLAAADQLNKMG 1878
            LGIIENPVSIK+IECAIIDKAFEEGWMVPRPPL R+GK+VAI+GSGPAGLAAADQLNKMG
Sbjct: 1826 LGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMG 1885

Query: 1879 HTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVNANIGTDPSYTLD 1938
            H VTVYER+DRIGGLMMYGVPNMKTDK+DVVQRRV+LM +EG+NFVVNANIG DPSY+LD
Sbjct: 1886 HLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLD 1945

Query: 1939 RLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDSNLENGNYISAKD 1998
             L++ENDA+VLAVG+TKPRDLPVPGR+L+GVHFAMEFLH+NTKSLLDSN E+GNYISAK 
Sbjct: 1946 GLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKG 2005

Query: 1999 KKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQWPRIFRVDYGHQ 2058
            KKVVVIGGGDTGTDCIGTSIRHGC+N+VNLELLPQPP TRAPGNPWPQWPR+FR+DYGHQ
Sbjct: 2006 KKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQ 2065

Query: 2059 EAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQFKEVEGSEEIIEA 2118
            EA  KFGKDPR+YEVLTKRFIGD+NG VKGLELVRV WEKD  GRFQFKE+EGSEEIIEA
Sbjct: 2066 EATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEA 2125

Query: 2119 DLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAGDCRRGQSLVVWA 2178
            DLV LAMGFLGPE T+AEKLG+E DNRSNFKAEYGRFST V+G+FAAGDCRRGQSLVVWA
Sbjct: 2126 DLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWA 2185

Query: 2179 INEGRQAAAQVDKYFSKEE 2192
            I+EGRQAA QVDK+ +K +
Sbjct: 2186 ISEGRQAADQVDKFLTKTD 2187

BLAST of MS000486 vs. TAIR 10
Match: AT5G53460.3 (NADH-dependent glutamate synthase 1 )

HSP 1 Score: 3702.5 bits (9600), Expect = 0.0e+00
Identity = 1822/2179 (83.62%), Postives = 1988/2179 (91.23%), Query Frame = 0

Query: 19   DKTSVKPHLNVTP-KARLGTRAARCSASKGATRSLNVSEKKFFGARLRAPGSGRIQFWHS 78
            + TSV   L VT   +R  +  ARCS  K       + E  F G R+R  GS  +QFW S
Sbjct: 26   NSTSVASQLAVTSGVSRRRSCTARCSVKKPV-----IPESPFLGTRVRRSGSETLQFWRS 85

Query: 79   DGPGRSPKLRLVVRSGLSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEM 138
            DGPGRS KLR VV+S  SAVPEKPLGLYDPS+DKDSCGVGFVAELSGE++RKT+TD+LEM
Sbjct: 86   DGPGRSAKLRTVVKSSFSAVPEKPLGLYDPSYDKDSCGVGFVAELSGETTRKTVTDSLEM 145

Query: 139  LVRMSHRGACGCETNTGDGAGILVALPHEYFKEARYILCAAKDNGFELPPPGQYAVGMFF 198
            L+RM+HRGACGCE+NTGDGAGILV LPH+++ E      AA + GF LP  G YAVGMFF
Sbjct: 146  LIRMTHRGACGCESNTGDGAGILVGLPHDFYAE------AATELGFVLPSAGNYAVGMFF 205

Query: 199  LPTSDSRREESKRVFTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTR 258
            LPT +SRREESK VFTKVAESLGHSVLGWR V TDN+GLG SAL TEP+I QVFLTP+T+
Sbjct: 206  LPTVESRREESKNVFTKVAESLGHSVLGWRLVPTDNSGLGNSALQTEPIIAQVFLTPTTK 265

Query: 259  SKVDLEKQMYILRRLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKD-YY 318
            SK D E+QMYILRR+SMVAIRAALNL+HG  +DFYICSLSSRTIVYKGQLKP QLKD YY
Sbjct: 266  SKADFEQQMYILRRVSMVAIRAALNLQHGAMKDFYICSLSSRTIVYKGQLKPDQLKDYYY 325

Query: 319  LDLGNERFTSYMALIHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLK 378
             DLG+ERFTSYMAL+HSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWM+AREGLLK
Sbjct: 326  ADLGSERFTSYMALVHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMRAREGLLK 385

Query: 379  CQELGLSEDELKHLLPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNM 438
            C ELGLS+ ELK LLPIVD SSSDSGAFDGVLELL+RAGRSLPEAVMMMIPEAWQNDKN+
Sbjct: 386  CNELGLSKKELKKLLPIVDVSSSDSGAFDGVLELLVRAGRSLPEAVMMMIPEAWQNDKNI 445

Query: 439  DPQRKALYEYFSCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEV 498
            DP RK  YEY S LMEPWDGPALISFTDGRYLGATLDRNGLRPGRFY+THSGRVIMASEV
Sbjct: 446  DPSRKEFYEYLSALMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYITHSGRVIMASEV 505

Query: 499  GVVDIAPEDVSRKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKD 558
            GVVD+ PEDV RKGRLNPGMMLLVDFE H+VVDD+ALKQQYSLARPYGEWLK+QKIELKD
Sbjct: 506  GVVDVPPEDVMRKGRLNPGMMLLVDFEKHIVVDDDALKQQYSLARPYGEWLKRQKIELKD 565

Query: 559  IVGSVDKSEMTPPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKD 618
            I+ SV ++E   P I+G +P S D+D+ME+MGIHGLL+PLKAFGYT EALEMLLLPMAKD
Sbjct: 566  IIESVPEAERIAPSISGVVPASNDDDSMESMGIHGLLSPLKAFGYTVEALEMLLLPMAKD 625

Query: 619  GVEALGSMGNDTPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGD 678
            G EALGSMGNDTPLAVMS+REKL FEYFKQMFAQVTNPPIDPIREKIVTSM+CMIGPEGD
Sbjct: 626  GSEALGSMGNDTPLAVMSNREKLCFEYFKQMFAQVTNPPIDPIREKIVTSMECMIGPEGD 685

Query: 679  LTETTEEQCHRLSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITYPKYLGRRGLEETLDRI 738
            LTETTEEQCHRLSLKGPLL IE+MEAIK+MNYRGWR+KVLDITY K  G +GLEETLDRI
Sbjct: 686  LTETTEEQCHRLSLKGPLLKIEEMEAIKKMNYRGWRTKVLDITYAKERGTKGLEETLDRI 745

Query: 739  CAEAHDAIKEGYTTLVLSDRAFSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEP 798
            C EA++AIKEGYT LVLSDRAFS  RVAVSSL+AVGAVH +LV  L RTQVGL++ESAEP
Sbjct: 746  CDEANEAIKEGYTLLVLSDRAFSATRVAVSSLMAVGAVHHHLVKTLARTQVGLVVESAEP 805

Query: 799  REVHHFCTLVGFGADAICPYLAVEAIWRLQVDGKIPAKSSGELHTKEELVKKYFKASNYG 858
            REVHHFCTLVGFGADAICPYLAVEA++RLQVDGKIP KS+GE H+KEELVKKY+KASNYG
Sbjct: 806  REVHHFCTLVGFGADAICPYLAVEAVYRLQVDGKIPPKSNGEFHSKEELVKKYYKASNYG 865

Query: 859  MMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHE 918
            MMKVLAKMGISTLASYKGAQIFEALGLSSEV++KCFAGTPSRVEGATFEMLARD L LHE
Sbjct: 866  MMKVLAKMGISTLASYKGAQIFEALGLSSEVIQKCFAGTPSRVEGATFEMLARDGLQLHE 925

Query: 919  MAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSK 978
            +AFPTR +  GSAEA AL NPG+YHWRK GE+HLNDP+AIAKLQEAARTNSV AYKEYSK
Sbjct: 926  LAFPTRGYAPGSAEASALTNPGNYHWRKNGEIHLNDPLAIAKLQEAARTNSVAAYKEYSK 985

Query: 979  LVHELNKACNLRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1038
             ++ELNK  NLRGL+KFK+  V IPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA
Sbjct: 986  RINELNKQSNLRGLMKFKDADVKIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMA 1045

Query: 1039 MNKIGGKSNTGEGGEQPSRMVPLPDGSMNPKRSAIKQVASGRFGVSSYYLTNADELQIKM 1098
            MNK+GGKSNTGEGGE PSRM PL DGS NPKRS+IKQ+ASGRFGVSSYYLTNADELQIKM
Sbjct: 1046 MNKLGGKSNTGEGGELPSRMEPLADGSRNPKRSSIKQIASGRFGVSSYYLTNADELQIKM 1105

Query: 1099 AQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNSNP 1158
            AQGAKPGEGGELPGHKV+G+IA TRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKN+NP
Sbjct: 1106 AQGAKPGEGGELPGHKVIGDIAITRNSTAGVGLISPPPHHDIYSIEDLAQLIHDLKNANP 1165

Query: 1159 AARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWELGLAET 1218
             ARISVKLVSEAGVGVIASGVVKGHADHVLI+GHDGGTGASRWTGIKNAGLPWELGLAET
Sbjct: 1166 GARISVKLVSEAGVGVIASGVVKGHADHVLIAGHDGGTGASRWTGIKNAGLPWELGLAET 1225

Query: 1219 HQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRKCHKNT 1278
            HQTLVANDLRGRTVLQTDGQLKTGRDVA+AALLGAEEFGFSTAPLIT+GCIMMRKCHKNT
Sbjct: 1226 HQTLVANDLRGRTVLQTDGQLKTGRDVAVAALLGAEEFGFSTAPLITLGCIMMRKCHKNT 1285

Query: 1279 CPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDLLEVDK 1338
            CPVGIATQDPVLREKFAGEPEHVINFFFM+AEE+REIMS LG RTV +M+GR+D+LE+D+
Sbjct: 1286 CPVGIATQDPVLREKFAGEPEHVINFFFMLAEEVREIMSGLGFRTVTEMIGRADMLELDR 1345

Query: 1339 EVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQDHGLDMALDQKLIALSKSAIEKSL 1398
            EV   N+KLENIDLSLLLRPAA++RP AAQ+CVQKQDHGLDMALDQ+LIALSKSA+EKSL
Sbjct: 1346 EVVKNNDKLENIDLSLLLRPAAEIRPGAAQYCVQKQDHGLDMALDQELIALSKSALEKSL 1405

Query: 1399 PVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAFLCPGIMLE 1458
            PVYIETPI NVNRAVGTMLSHEVTKRYH+ GLP +TIHIKFTGSAGQSLGAFLCPGIMLE
Sbjct: 1406 PVYIETPICNVNRAVGTMLSHEVTKRYHLTGLPKDTIHIKFTGSAGQSLGAFLCPGIMLE 1465

Query: 1459 LEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFNGMAAERFC 1518
            LEGDSNDYVGKGLSGGK+VVYPPKGS FDPKENI+IGNVALYGATSGEAYFNGMAAERF 
Sbjct: 1466 LEGDSNDYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFS 1525

Query: 1519 VRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDGKFESRCNL 1578
            VRNSGAKAVVEG+GDHGCEYMTGGTVVVLG TGRNFAAGMSGG+AYVLD+DGKF +RCNL
Sbjct: 1526 VRNSGAKAVVEGLGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVDGKFNTRCNL 1585

Query: 1579 ELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFPREYKRVLA 1638
            ELVDLDKVE+E+D +TLKMMIQQHQRHT+S LA+EVL +FENLLP+FIKVFPR+YKRVL+
Sbjct: 1586 ELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLADFENLLPKFIKVFPRDYKRVLS 1645

Query: 1639 NMKAQEAAKEASEPAAKEAEEESDEAELIEKDAFEELKKMAAAS----LNGNSKQVEETE 1698
             MK +E +K+A E A++EA +E++E EL EKDAF ELK MAAAS    ++GN    E   
Sbjct: 1646 AMKHEEVSKQAIERASEEA-DETEEKELEEKDAFAELKNMAAASSKEEMSGNGVAAE--- 1705

Query: 1699 PPKRPTEIPDAVKHRGFIAYEREGVKYRDPNVRMGDWKEVMEESKPGPLLKTQSARCMDC 1758
               RP+++ +AVK+ GFIAYEREGVKYRDPNVR+ DW EVMEESKPGPLL TQSARCMDC
Sbjct: 1706 --ARPSKVDNAVKNGGFIAYEREGVKYRDPNVRLNDWNEVMEESKPGPLLTTQSARCMDC 1765

Query: 1759 GTPFCHQENSGCPLGNKIPEFNELVYQNRWREALERLLETNNFPEFTGRVCPAPCEGSCV 1818
            GTPFCHQENSGCPLGNKIPEFNELVYQNRW+EAL RLLETNNFPEFTGRVCPAPCEGSCV
Sbjct: 1766 GTPFCHQENSGCPLGNKIPEFNELVYQNRWQEALNRLLETNNFPEFTGRVCPAPCEGSCV 1825

Query: 1819 LGIIENPVSIKNIECAIIDKAFEEGWMVPRPPLTRSGKRVAIVGSGPAGLAAADQLNKMG 1878
            LGIIENPVSIK+IECAIIDKAFEEGWMVPRPPL R+GK+VAI+GSGPAGLAAADQLNKMG
Sbjct: 1826 LGIIENPVSIKSIECAIIDKAFEEGWMVPRPPLKRTGKKVAIIGSGPAGLAAADQLNKMG 1885

Query: 1879 HTVTVYERADRIGGLMMYGVPNMKTDKVDVVQRRVNLMAEEGVNFVVNANIGTDPSYTLD 1938
            H VTVYER+DRIGGLMMYGVPNMKTDK+DVVQRRV+LM +EG+NFVVNANIG DPSY+LD
Sbjct: 1886 HLVTVYERSDRIGGLMMYGVPNMKTDKIDVVQRRVDLMTKEGINFVVNANIGKDPSYSLD 1945

Query: 1939 RLRDENDALVLAVGATKPRDLPVPGRELAGVHFAMEFLHSNTKSLLDSNLENGNYISAKD 1998
             L++ENDA+VLAVG+TKPRDLPVPGR+L+GVHFAMEFLH+NTKSLLDSN E+GNYISAK 
Sbjct: 1946 GLKEENDAIVLAVGSTKPRDLPVPGRDLSGVHFAMEFLHANTKSLLDSNHEDGNYISAKG 2005

Query: 1999 KKVVVIGGGDTGTDCIGTSIRHGCSNVVNLELLPQPPQTRAPGNPWPQWPRIFRVDYGHQ 2058
            KKVVVIGGGDTGTDCIGTSIRHGC+N+VNLELLPQPP TRAPGNPWPQWPR+FR+DYGHQ
Sbjct: 2006 KKVVVIGGGDTGTDCIGTSIRHGCTNIVNLELLPQPPSTRAPGNPWPQWPRVFRIDYGHQ 2065

Query: 2059 EAAAKFGKDPRSYEVLTKRFIGDENGAVKGLELVRVQWEKDANGRFQFKEVEGSEEIIEA 2118
            EA  KFGKDPR+YEVLTKRFIGD+NG VKGLELVRV WEKD  GRFQFKE+EGSEEIIEA
Sbjct: 2066 EATTKFGKDPRTYEVLTKRFIGDDNGNVKGLELVRVSWEKDETGRFQFKEIEGSEEIIEA 2125

Query: 2119 DLVLLAMGFLGPESTVAEKLGVEKDNRSNFKAEYGRFSTNVDGIFAAGDCRRGQSLVVWA 2178
            DLV LAMGFLGPE T+AEKLG+E DNRSNFKAEYGRFST V+G+FAAGDCRRGQSLVVWA
Sbjct: 2126 DLVFLAMGFLGPEPTLAEKLGLECDNRSNFKAEYGRFSTTVEGVFAAGDCRRGQSLVVWA 2185

Query: 2179 INEGRQAAAQVDKYFSKEE 2192
            I+EGRQAA QVDK+ +K +
Sbjct: 2186 ISEGRQAADQVDKFLTKTD 2187

BLAST of MS000486 vs. TAIR 10
Match: AT5G04140.1 (glutamate synthase 1 )

HSP 1 Score: 1212.6 bits (3136), Expect = 0.0e+00
Identity = 699/1572 (44.47%), Postives = 953/1572 (60.62%), Query Frame = 0

Query: 92   SGLSAVPEKPLGLYDPSFDKDSCGVGFVAELSGESSRKTITDALEMLVRMSHRGACGCET 151
            S  SA+  +   L D   ++ +CGVGF+A L    S   + DAL  L  M HRG CG + 
Sbjct: 84   SSPSALRPQVANLEDILSERGACGVGFIANLDNIPSHGVVKDALIALGCMEHRGGCGADN 143

Query: 152  NTGDGAGILVALPHEYFKEARYILCAAKDNGFELPPPGQYAVGMFFLPTSDSRREESKRV 211
            ++GDG+G++ ++P ++F         AK+            VGM FLP  D+  +E+K+V
Sbjct: 144  DSGDGSGLMSSIPWDFFN------VWAKEQSLAPFDKLHTGVGMIFLPQDDTFMQEAKQV 203

Query: 212  FTKVAESLGHSVLGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTR-SKVDLEKQMYILR 271
               + E  G  VLGWR V  +   +GK+A  T P I+QVF+  +   S  D+E+++YI R
Sbjct: 204  IENIFEKEGLQVLGWREVPVNVPIVGKNARETMPNIQQVFVKIAKEDSTDDIERELYICR 263

Query: 272  RLSMVAIRAALNLEHGGARDFYICSLSSRTIVYKGQLKPVQLKDYYLDLGNERFTSYMAL 331
            +L    I  A+  E  G  + Y CSLS++TIVYKG L+   L  +YLDL NE + S  A+
Sbjct: 264  KL----IERAVATESWGT-ELYFCSLSNQTIVYKGMLRSEALGLFYLDLQNELYESPFAI 323

Query: 332  IHSRFSTNTFPSWDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLKCQELGLSEDELKHL 391
             H R+STNT P W  AQPMR LGHNGEINT++GN+NWM++RE  LK       E+E++  
Sbjct: 324  YHRRYSTNTSPRWPLAQPMRFLGHNGEINTIQGNLNWMQSREASLKAAVWNGRENEIR-- 383

Query: 392  LPIVDASSSDSGAFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMD---PQRKALYEYF 451
             P  +   SDS   D   E++IR+GR+  EA+M+++PEA++N   +    P+    Y+Y+
Sbjct: 384  -PFGNPRGSDSANLDSAAEIMIRSGRTPEEALMILVPEAYKNHPTLSVKYPEVVDFYDYY 443

Query: 452  SCLMEPWDGPALISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVDIAPEDVS 511
               ME WDGPAL+ F+DG+ +GA LDRNGLRP R++ T    V +ASEVGVV +    V+
Sbjct: 444  KGQMEAWDGPALLLFSDGKTVGACLDRNGLRPARYWRTSDNFVYVASEVGVVPVDEAKVT 503

Query: 512  RKGRLNPGMMLLVDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKDIVGSVDKSEMT 571
             KGRL PGMM+ VD  N  V ++  +K++ S   PYG+W+K+    LK +          
Sbjct: 504  MKGRLGPGMMIAVDLVNGQVYENTEVKKRISSFNPYGKWIKENSRFLKPV---------- 563

Query: 572  PPVIAGALPTSTDEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKDGVEALGSMGND 631
                     +ST  +N E      +L   +AFGY++E ++M++  MA  G E    MG+D
Sbjct: 564  ------NFKSSTVMENEE------ILRSQQAFGYSSEDVQMVIESMASQGKEPTFCMGDD 623

Query: 632  TPLAVMSDREKLTFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGDLTETTEEQCHR 691
             PLA +S R  + ++YFKQ FAQVTNP IDP+RE +V S++  IG  G++ E   E   +
Sbjct: 624  IPLAGLSQRPHMLYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILELGPENASQ 683

Query: 692  LSLKGPLLSIEQMEAIKRMNYRGWRSKVLDITYPKYLGRRG-LEETLDRICAEAHDAIKE 751
            + L  P+L+   +E + +  Y   + KVL   +    G  G L++ L  +C  A DA++ 
Sbjct: 684  VILSNPVLNEGALEELMKDQY--LKPKVLSTYFDIRKGVEGSLQKALYYLCEAADDAVRS 743

Query: 752  GYTTLVLSDRA--FSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEPREVHHFCT 811
            G   LVLSDR+      R ++  +LAVGAVHQ+L+ N  R    ++ ++A+    HHF  
Sbjct: 744  GSQLLVLSDRSDRLEPTRPSIPIMLAVGAVHQHLIQNGLRMSASIVADTAQCFSTHHFAC 803

Query: 812  LVGFGADAICPYLAVEAI--WRL-------QVDGKIPAKSSGELHTKEELVKKYFKASNY 871
            LVG+GA A+CPYLA+E    WRL         +GKIP        T E+  K Y KA N 
Sbjct: 804  LVGYGASAVCPYLALETCRQWRLSNKTVAFMRNGKIPTV------TIEQAQKNYTKAVNA 863

Query: 872  GMMKVLAKMGISTLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLH 931
            G++K+L+KMGIS L+SY GAQIFE  GL  +VV+  F G+ S++ G TF+ LAR+ L+  
Sbjct: 864  GLLKILSKMGISLLSSYCGAQIFEIYGLGQDVVDLAFTGSVSKISGLTFDELARETLSFW 923

Query: 932  EMAFPTRLFPAGSAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYS 991
              AF              L N G   +R GGE H N+P     L +A R  S  AY  Y 
Sbjct: 924  VKAF-------SEDTTKRLENFGFIQFRPGGEYHSNNPEMSKLLHKAVREKSETAYAVYQ 983

Query: 992  KLVHELNKACN-LRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLA 1051
            +  H  N+  N LR LL+FK     IP+ +VEPA  IV+RFCTG MS G+IS E H  +A
Sbjct: 984  Q--HLSNRPVNVLRDLLEFKSDRAPIPVGKVEPAVAIVQRFCTGGMSLGAISRETHEAIA 1043

Query: 1052 MAMNKIGGKSNTGEGGEQPSRMVPLPD-------------GSMNP--KRSAIKQVASGRF 1111
            +AMN+IGGKSN+GEGGE P R  PL D             G  N     SAIKQVASGRF
Sbjct: 1044 IAMNRIGGKSNSGEGGEDPIRWKPLTDVVDGYSPTLPHLKGLQNGDIATSAIKQVASGRF 1103

Query: 1112 GVSSYYLTNADELQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIY 1171
            GV+  +L NAD+L+IK+AQGAKPGEGG+LPG KV   IA  R+S  GV LISPPPHHDIY
Sbjct: 1104 GVTPTFLVNADQLEIKVAQGAKPGEGGQLPGKKVSAYIARLRSSKPGVPLISPPPHHDIY 1163

Query: 1172 SIEDLAQLIHDLKNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRW 1231
            SIEDLAQLI DL   NP A++SVKLV+EAG+G +ASGV KG+AD + ISGHDGGTGAS  
Sbjct: 1164 SIEDLAQLIFDLHQINPNAKVSVKLVAEAGIGTVASGVAKGNADIIQISGHDGGTGASPI 1223

Query: 1232 TGIKNAGLPWELGLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTA 1291
            + IK+AG PWELGL ETHQTL+AN LR R +L+ DG LK+G DV +AA +GA+E+GF + 
Sbjct: 1224 SSIKHAGGPWELGLTETHQTLIANGLRERVILRVDGGLKSGVDVLMAAAMGADEYGFGSL 1283

Query: 1292 PLITMGCIMMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGV 1351
             +I  GC+M R CH N CPVG+A+Q   LR +F G P  ++N+F  VAEE+R I++QLG 
Sbjct: 1284 AMIATGCVMARICHTNNCPVGVASQREELRARFPGVPGDLVNYFLYVAEEVRGILAQLGY 1343

Query: 1352 RTVNQMVGRSDLLEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQD-HGLDM 1411
             +++ ++GR++LL   ++++    K +++DLS LL       P  +   ++KQ+ H    
Sbjct: 1344 NSLDDIIGRTELLR-PRDISL--VKTQHLDLSYLLSSVG--TPSLSSTEIRKQEVHTNGP 1403

Query: 1412 ALDQKLIA--LSKSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIK 1471
             LD  ++A  L   AIE    V     I NV+RA    ++  + K+Y   G   + +++ 
Sbjct: 1404 VLDDDILADPLVIDAIENEKVVEKTVKICNVDRAACGRVAGVIAKKYGDTGFAGQ-VNLT 1463

Query: 1472 FTGSAGQSLGAFLCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVA 1531
            F GSAGQS G FL PG+ + L G+SNDYVGKG++GG+IVV P +   F P+E  I+GN  
Sbjct: 1464 FLGSAGQSFGCFLIPGMNIRLIGESNDYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTC 1523

Query: 1532 LYGATSGEAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGM 1591
            LYGAT G+ +  G A ERF VRNS A+AVVEG GDH CEYMTGG VVVLG  GRN AAGM
Sbjct: 1524 LYGATGGQIFARGKAGERFAVRNSLAEAVVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGM 1583

Query: 1592 SGGVAYVLDIDGKFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENF 1629
            +GG+AY+LD D     + N E+V + +V      L LK +I+ H   T S+    +L  +
Sbjct: 1584 TGGLAYLLDEDDTLLPKINREIVKIQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNEW 1596

BLAST of MS000486 vs. TAIR 10
Match: AT5G04140.2 (glutamate synthase 1 )

HSP 1 Score: 1210.3 bits (3130), Expect = 0.0e+00
Identity = 696/1560 (44.62%), Postives = 948/1560 (60.77%), Query Frame = 0

Query: 104  LYDPSFDKDSCGVGFVAELSGESSRKTITDALEMLVRMSHRGACGCETNTGDGAGILVAL 163
            L D   ++ +CGVGF+A L    S   + DAL  L  M HRG CG + ++GDG+G++ ++
Sbjct: 122  LEDILSERGACGVGFIANLDNIPSHGVVKDALIALGCMEHRGGCGADNDSGDGSGLMSSI 181

Query: 164  PHEYFKEARYILCAAKDNGFELPPPGQYAVGMFFLPTSDSRREESKRVFTKVAESLGHSV 223
            P ++F         AK+            VGM FLP  D+  +E+K+V   + E  G  V
Sbjct: 182  PWDFFN------VWAKEQSLAPFDKLHTGVGMIFLPQDDTFMQEAKQVIENIFEKEGLQV 241

Query: 224  LGWRSVETDNTGLGKSALLTEPVIEQVFLTPSTR-SKVDLEKQMYILRRLSMVAIRAALN 283
            LGWR V  +   +GK+A  T P I+QVF+  +   S  D+E+++YI R+L    I  A+ 
Sbjct: 242  LGWREVPVNVPIVGKNARETMPNIQQVFVKIAKEDSTDDIERELYICRKL----IERAVA 301

Query: 284  LEHGGARDFYICSLSSRTIVYKGQLKPVQLKDYYLDLGNERFTSYMALIHSRFSTNTFPS 343
             E  G  + Y CSLS++TIVYKG L+   L  +YLDL NE + S  A+ H R+STNT P 
Sbjct: 302  TESWGT-ELYFCSLSNQTIVYKGMLRSEALGLFYLDLQNELYESPFAIYHRRYSTNTSPR 361

Query: 344  WDRAQPMRVLGHNGEINTLRGNVNWMKAREGLLKCQELGLSEDELKHLLPIVDASSSDSG 403
            W  AQPMR LGHNGEINT++GN+NWM++RE  LK       E+E++   P  +   SDS 
Sbjct: 362  WPLAQPMRFLGHNGEINTIQGNLNWMQSREASLKAAVWNGRENEIR---PFGNPRGSDSA 421

Query: 404  AFDGVLELLIRAGRSLPEAVMMMIPEAWQNDKNMD---PQRKALYEYFSCLMEPWDGPAL 463
              D   E++IR+GR+  EA+M+++PEA++N   +    P+    Y+Y+   ME WDGPAL
Sbjct: 422  NLDSAAEIMIRSGRTPEEALMILVPEAYKNHPTLSVKYPEVVDFYDYYKGQMEAWDGPAL 481

Query: 464  ISFTDGRYLGATLDRNGLRPGRFYVTHSGRVIMASEVGVVDIAPEDVSRKGRLNPGMMLL 523
            + F+DG+ +GA LDRNGLRP R++ T    V +ASEVGVV +    V+ KGRL PGMM+ 
Sbjct: 482  LLFSDGKTVGACLDRNGLRPARYWRTSDNFVYVASEVGVVPVDEAKVTMKGRLGPGMMIA 541

Query: 524  VDFENHVVVDDEALKQQYSLARPYGEWLKKQKIELKDIVGSVDKSEMTPPVIAGALPTST 583
            VD  N  V ++  +K++ S   PYG+W+K+    LK +                   +ST
Sbjct: 542  VDLVNGQVYENTEVKKRISSFNPYGKWIKENSRFLKPV----------------NFKSST 601

Query: 584  DEDNMENMGIHGLLAPLKAFGYTTEALEMLLLPMAKDGVEALGSMGNDTPLAVMSDREKL 643
              +N E      +L   +AFGY++E ++M++  MA  G E    MG+D PLA +S R  +
Sbjct: 602  VMENEE------ILRSQQAFGYSSEDVQMVIESMASQGKEPTFCMGDDIPLAGLSQRPHM 661

Query: 644  TFEYFKQMFAQVTNPPIDPIREKIVTSMQCMIGPEGDLTETTEEQCHRLSLKGPLLSIEQ 703
             ++YFKQ FAQVTNP IDP+RE +V S++  IG  G++ E   E   ++ L  P+L+   
Sbjct: 662  LYDYFKQRFAQVTNPAIDPLREGLVMSLEVNIGKRGNILELGPENASQVILSNPVLNEGA 721

Query: 704  MEAIKRMNYRGWRSKVLDITYPKYLGRRG-LEETLDRICAEAHDAIKEGYTTLVLSDRA- 763
            +E + +  Y   + KVL   +    G  G L++ L  +C  A DA++ G   LVLSDR+ 
Sbjct: 722  LEELMKDQY--LKPKVLSTYFDIRKGVEGSLQKALYYLCEAADDAVRSGSQLLVLSDRSD 781

Query: 764  -FSTKRVAVSSLLAVGAVHQYLVNNLERTQVGLIIESAEPREVHHFCTLVGFGADAICPY 823
                 R ++  +LAVGAVHQ+L+ N  R    ++ ++A+    HHF  LVG+GA A+CPY
Sbjct: 782  RLEPTRPSIPIMLAVGAVHQHLIQNGLRMSASIVADTAQCFSTHHFACLVGYGASAVCPY 841

Query: 824  LAVEAI--WRL-------QVDGKIPAKSSGELHTKEELVKKYFKASNYGMMKVLAKMGIS 883
            LA+E    WRL         +GKIP        T E+  K Y KA N G++K+L+KMGIS
Sbjct: 842  LALETCRQWRLSNKTVAFMRNGKIPTV------TIEQAQKNYTKAVNAGLLKILSKMGIS 901

Query: 884  TLASYKGAQIFEALGLSSEVVEKCFAGTPSRVEGATFEMLARDALNLHEMAFPTRLFPAG 943
             L+SY GAQIFE  GL  +VV+  F G+ S++ G TF+ LAR+ L+    AF        
Sbjct: 902  LLSSYCGAQIFEIYGLGQDVVDLAFTGSVSKISGLTFDELARETLSFWVKAF-------S 961

Query: 944  SAEAVALPNPGDYHWRKGGEVHLNDPVAIAKLQEAARTNSVNAYKEYSKLVHELNKACN- 1003
                  L N G   +R GGE H N+P     L +A R  S  AY  Y +  H  N+  N 
Sbjct: 962  EDTTKRLENFGFIQFRPGGEYHSNNPEMSKLLHKAVREKSETAYAVYQQ--HLSNRPVNV 1021

Query: 1004 LRGLLKFKETGVSIPLDEVEPASEIVKRFCTGAMSYGSISLEAHTTLAMAMNKIGGKSNT 1063
            LR LL+FK     IP+ +VEPA  IV+RFCTG MS G+IS E H  +A+AMN+IGGKSN+
Sbjct: 1022 LRDLLEFKSDRAPIPVGKVEPAVAIVQRFCTGGMSLGAISRETHEAIAIAMNRIGGKSNS 1081

Query: 1064 GEGGEQPSRMVPLPD-------------GSMNP--KRSAIKQVASGRFGVSSYYLTNADE 1123
            GEGGE P R  PL D             G  N     SAIKQVASGRFGV+  +L NAD+
Sbjct: 1082 GEGGEDPIRWKPLTDVVDGYSPTLPHLKGLQNGDIATSAIKQVASGRFGVTPTFLVNADQ 1141

Query: 1124 LQIKMAQGAKPGEGGELPGHKVVGEIASTRNSTAGVGLISPPPHHDIYSIEDLAQLIHDL 1183
            L+IK+AQGAKPGEGG+LPG KV   IA  R+S  GV LISPPPHHDIYSIEDLAQLI DL
Sbjct: 1142 LEIKVAQGAKPGEGGQLPGKKVSAYIARLRSSKPGVPLISPPPHHDIYSIEDLAQLIFDL 1201

Query: 1184 KNSNPAARISVKLVSEAGVGVIASGVVKGHADHVLISGHDGGTGASRWTGIKNAGLPWEL 1243
               NP A++SVKLV+EAG+G +ASGV KG+AD + ISGHDGGTGAS  + IK+AG PWEL
Sbjct: 1202 HQINPNAKVSVKLVAEAGIGTVASGVAKGNADIIQISGHDGGTGASPISSIKHAGGPWEL 1261

Query: 1244 GLAETHQTLVANDLRGRTVLQTDGQLKTGRDVAIAALLGAEEFGFSTAPLITMGCIMMRK 1303
            GL ETHQTL+AN LR R +L+ DG LK+G DV +AA +GA+E+GF +  +I  GC+M R 
Sbjct: 1262 GLTETHQTLIANGLRERVILRVDGGLKSGVDVLMAAAMGADEYGFGSLAMIATGCVMARI 1321

Query: 1304 CHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMSQLGVRTVNQMVGRSDL 1363
            CH N CPVG+A+Q   LR +F G P  ++N+F  VAEE+R I++QLG  +++ ++GR++L
Sbjct: 1322 CHTNNCPVGVASQREELRARFPGVPGDLVNYFLYVAEEVRGILAQLGYNSLDDIIGRTEL 1381

Query: 1364 LEVDKEVAWQNEKLENIDLSLLLRPAADLRPEAAQFCVQKQD-HGLDMALDQKLIA--LS 1423
            L   ++++    K +++DLS LL       P  +   ++KQ+ H     LD  ++A  L 
Sbjct: 1382 LR-PRDISL--VKTQHLDLSYLLSSVG--TPSLSSTEIRKQEVHTNGPVLDDDILADPLV 1441

Query: 1424 KSAIEKSLPVYIETPINNVNRAVGTMLSHEVTKRYHMAGLPLETIHIKFTGSAGQSLGAF 1483
              AIE    V     I NV+RA    ++  + K+Y   G   + +++ F GSAGQS G F
Sbjct: 1442 IDAIENEKVVEKTVKICNVDRAACGRVAGVIAKKYGDTGFAGQ-VNLTFLGSAGQSFGCF 1501

Query: 1484 LCPGIMLELEGDSNDYVGKGLSGGKIVVYPPKGSLFDPKENIIIGNVALYGATSGEAYFN 1543
            L PG+ + L G+SNDYVGKG++GG+IVV P +   F P+E  I+GN  LYGAT G+ +  
Sbjct: 1502 LIPGMNIRLIGESNDYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTCLYGATGGQIFAR 1561

Query: 1544 GMAAERFCVRNSGAKAVVEGVGDHGCEYMTGGTVVVLGSTGRNFAAGMSGGVAYVLDIDG 1603
            G A ERF VRNS A+AVVEG GDH CEYMTGG VVVLG  GRN AAGM+GG+AY+LD D 
Sbjct: 1562 GKAGERFAVRNSLAEAVVEGTGDHCCEYMTGGCVVVLGKVGRNVAAGMTGGLAYLLDEDD 1621

Query: 1604 KFESRCNLELVDLDKVEEEDDILTLKMMIQQHQRHTSSNLAKEVLENFENLLPRFIKVFP 1629
                + N E+V + +V      L LK +I+ H   T S+    +L  +E  LP F ++ P
Sbjct: 1622 TLLPKINREIVKIQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNEWEKYLPLFWQLVP 1622