MS000474 (gene) Bitter gourd (TR) v1

Overview
NameMS000474
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein-serine/threonine phosphatase
Locationscaffold44: 1950052 .. 1952784 (-)
RNA-Seq ExpressionMS000474
SyntenyMS000474
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATGCAGCTACAATATAAGGAATGTGATTGCTCAGGCTGGACATATAGGAATAATCAACAGACGGGTCCAAATCAAATATTTGTGGAGGAGCTTACCTGCCAATCTTGGATTTGCTCACACCTGGAAGCGTGAATTTAGAACAAGTCTCATGATGATGGTCGATACGGGTGCATCGGAAAATCGCGGGCTGATTGTTGATCAGCATCCAGAGAAAGACGAAGATGGTGGCTATGCCAGTGGAGGGTGGAAAAGGTCTGAATGGATTTCGTTTGAATGATTTTCATTTGAAGTTAAGCAGAATTGTGCTATTGAATTGTTATTCTGCTCCTACGAAGATAAAATGAACACAATGCTTTGATATTGTTGCTGCACCTAGCCTATTGAATAATGGATTTCAATGAGAAATGCACTTAGCTTTTTATCTTTTGGTTTGATTTTCAGCGATGATGGGAAACTTAGTTGGGGGTATTCAAGTTTCAGAGGAAAGAGGGCAACCATGGAAGATTTCTTTGATATTAAAATGTCAAAGGTCGATGGTCAAACAGTCTGCCTTTTTGGCATTTTTGATGGTTAGACTCTCCCTCCATTTTTCCAGTGGCGTGATGTAGGTGTTAGGTTGTCAGTAAATTCTAATCTTTCCAATTCAGTGGACTAGGTAGTGATTGAAGTTTAATAAATTGTATATATTCAAACTGCACCAAGTTTATATTGTATATATCCAAACCANAATTGATGTTGAACATTTAACGATATATGCTATCATGGATATTTTCAGATGTTAGAAATAGTAGAGATAATTACTTCTTTTTATGTTGTTATAGGTCACGGTGGATCCCGTGCTGCAGAGTTTCTGAAGGACCATCTCTTTGAGAATCTCATGAAGCATCCAAAATTTTTGATGGATACCAAATTAGCAATCAGTGTGTTTGCCTTCCCTTATGCCTCATCCTTTTGTTTACGAAGCTAAAAACATTAGGTTTATATGATGGTTATATTATAGCTACCCACATGTGTAACAGTGCAGGATCTTTAAGTTTTATGTTCTTTGTACACATTGCACTGTTTTATATTGAAAACAAAATTCTAAGATTCCAGTTCTCATAATTTTCTACTGATTAAAAATTTGTTCTATCACTTCACAGGTGAAACTTATCAACAGACCGATGCTGAATTTTTGAATTCAGAAAAAGATACTTTAAGGGACGATGGCTCTACTGCTTCAACAGCTTTGCTAGTTGGTAATCACCTCTATGTTGCAAATGTTGGAGATTCACGTGCTATAATATCAAAGGGTGGTGAAGGTATTACTTCTTTATTTTTTTCTTGTTTTCTATGCTTTTTAGTTTTAATGTAAGGCAGAAAGTCAAGACTCTTGACCATAATAGTTTCTAGTGAAAGCTAATAATAAAGGTAAAGTTAAACAAATGGGAATTCAAAATGTACCTTCATATGCTTGGTCAGTGACTTTGTGCTTATTTTTTGAATGTAAACTTTGAAACGAATTTTAATTCTTCTGGGCAGCTATCCCCCTTTCTGAGGACCATAAGCCGAACAGAACTGACGAGCGAAGGAGAATTGAAAACGCCGGGGGTGTTGTCATGTGGGCTGGTAAGATTAATAATGGCTTTGGTAATGATGTTAATAACTGCTATCCCTGTTGGTTAACTTGGAAAATGATTAGTGCTAACTTGAAATATATAAACAAGGGACTCAAAGTTCCATCATCACATTTACACAGACACTCTAATAAGAATGATTCACATGCTAATGGAAGTTCTGAGCACTGAAAGCTGAAATATGTATCCTAAACCAAAAACCGAGTCATCCTGTTCGTTTTAACTACTATGCAAGATTTCCTTGATAACTACGGATTGGTTGCCTGAACAATATAATTCTTGTTACCTACTTCTGGAAAGAATGTAATGCCTTTAGTTCATTTTCTGCTTCGATATTGCAATAATCCGGAGGTGATTACATCATAGGTCTGTCTTAATCACATTGTATTCTGTTGTTGTTAGGTACTTGGAGGGTAGGAGGAGTTTTAGCAATGTCCCGTGCTTTTGGTAACAAGATGTTGAAGCAGTTTGTCGTGGCAGATCCCGAGATTCAGGTGTTTTTCCCAATACCCTGTCGTCCAGGCAATCATTTTAGTTCCAAAAAATAGTTATTTTTGTTAACCTATGTGCAGGGGAGCCCAAATAAGTATTAGGTTCACCTTGTGATTGGTAGTGTTAGACCAATAATGGAATCATGGAATGGATAGATGGAAGGAGAAAGTCTTGGATCTTCTCAAATTTTTAGTAGCTTGAATAGACTTAGAATGTTTTATGGAGATCTGAATGTTTCAACTAGCGAATTTCACATGTAGTAAAGGTTGTTGTGGTTTACGAACTGACAACAATGCTGATCAGGATCTCGAAGTTGACAAAGACATTGAGTTGCTCGTTCTCGCAAGCGATGGACTTTGGGACGTGGTACGCAACGAGGTATGTGCTCTCTCCCATTCATCCAAGATGCTGTAAATGGCTATATGATCTCCACATATGAACATAAATGCCGAATTCAGGATGCAGTATTAGTTGCTGGGAAGGAGGAAGAACCCGAGGCAGCTGCTCGGAAGCTAACCGAAGCAGCATTTACCCGTGGTAGTGCTGACAACATAACGTGCATTGTGGTGAAGTTCCATCACGGCAATGCAGGTCCCGCTGCCGATACTCGAGATGCGGAC

mRNA sequence

ATGGTATGCAGCTACAATATAAGGAATGTGATTGCTCAGGCTGGACATATAGGAATAATCAACAGACGGGTCCAAATCAAATATTTGTGGAGGAGCTTACCTGCCAATCTTGGATTTGCTCACACCTGGAAGCGTGAATTTAGAACAAGTCTCATGATGATGGTCGATACGGGTGCATCGGAAAATCGCGGGCTGATTGTTGATCAGCATCCAGAGAAAGACGAAGATGGTGGCTATGCCAGTGGAGGGTGGAAAAGCGATGATGGGAAACTTAGTTGGGGGTATTCAAGTTTCAGAGGAAAGAGGGCAACCATGGAAGATTTCTTTGATATTAAAATGTCAAAGGTCGATGGTCAAACAGTCTGCCTTTTTGGCATTTTTGATGGTCACGGTGGATCCCGTGCTGCAGAGTTTCTGAAGGACCATCTCTTTGAGAATCTCATGAAGCATCCAAAATTTTTGATGGATACCAAATTAGCAATCAGTGAAACTTATCAACAGACCGATGCTGAATTTTTGAATTCAGAAAAAGATACTTTAAGGGACGATGGCTCTACTGCTTCAACAGCTTTGCTAGTTGGTAATCACCTCTATGTTGCAAATGTTGGAGATTCACGTGCTATAATATCAAAGGGTGGTGAAGCTATCCCCCTTTCTGAGGACCATAAGCCGAACAGAACTGACGAGCGAAGGAGAATTGAAAACGCCGGGGGTGTTGTCATGTGGGCTGGTACTTGGAGGGTAGGAGGAGTTTTAGCAATGTCCCGTGCTTTTGGTAACAAGATGTTGAAGCAGTTTGTCGTGGCAGATCCCGAGATTCAGGATCTCGAAGTTGACAAAGACATTGAGTTGCTCGTTCTCGCAAGCGATGGACTTTGGGACGTGGTACGCAACGAGGATGCAGTATTAGTTGCTGGGAAGGAGGAAGAACCCGAGGCAGCTGCTCGGAAGCTAACCGAAGCAGCATTTACCCGTGGTAGTGCTGACAACATAACGTGCATTGTGGTGAAGTTCCATCACGGCAATGCAGGTCCCGCTGCCGATACTCGAGATGCGGAC

Coding sequence (CDS)

ATGGTATGCAGCTACAATATAAGGAATGTGATTGCTCAGGCTGGACATATAGGAATAATCAACAGACGGGTCCAAATCAAATATTTGTGGAGGAGCTTACCTGCCAATCTTGGATTTGCTCACACCTGGAAGCGTGAATTTAGAACAAGTCTCATGATGATGGTCGATACGGGTGCATCGGAAAATCGCGGGCTGATTGTTGATCAGCATCCAGAGAAAGACGAAGATGGTGGCTATGCCAGTGGAGGGTGGAAAAGCGATGATGGGAAACTTAGTTGGGGGTATTCAAGTTTCAGAGGAAAGAGGGCAACCATGGAAGATTTCTTTGATATTAAAATGTCAAAGGTCGATGGTCAAACAGTCTGCCTTTTTGGCATTTTTGATGGTCACGGTGGATCCCGTGCTGCAGAGTTTCTGAAGGACCATCTCTTTGAGAATCTCATGAAGCATCCAAAATTTTTGATGGATACCAAATTAGCAATCAGTGAAACTTATCAACAGACCGATGCTGAATTTTTGAATTCAGAAAAAGATACTTTAAGGGACGATGGCTCTACTGCTTCAACAGCTTTGCTAGTTGGTAATCACCTCTATGTTGCAAATGTTGGAGATTCACGTGCTATAATATCAAAGGGTGGTGAAGCTATCCCCCTTTCTGAGGACCATAAGCCGAACAGAACTGACGAGCGAAGGAGAATTGAAAACGCCGGGGGTGTTGTCATGTGGGCTGGTACTTGGAGGGTAGGAGGAGTTTTAGCAATGTCCCGTGCTTTTGGTAACAAGATGTTGAAGCAGTTTGTCGTGGCAGATCCCGAGATTCAGGATCTCGAAGTTGACAAAGACATTGAGTTGCTCGTTCTCGCAAGCGATGGACTTTGGGACGTGGTACGCAACGAGGATGCAGTATTAGTTGCTGGGAAGGAGGAAGAACCCGAGGCAGCTGCTCGGAAGCTAACCGAAGCAGCATTTACCCGTGGTAGTGCTGACAACATAACGTGCATTGTGGTGAAGTTCCATCACGGCAATGCAGGTCCCGCTGCCGATACTCGAGATGCGGAC

Protein sequence

MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGASENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD
Homology
BLAST of MS000474 vs. NCBI nr
Match: XP_022140944.1 (probable protein phosphatase 2C 76 [Momordica charantia] >XP_022140945.1 probable protein phosphatase 2C 76 [Momordica charantia])

HSP 1 Score: 710.3 bits (1832), Expect = 8.3e-201
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD 354
           AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD
Sbjct: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD 353

BLAST of MS000474 vs. NCBI nr
Match: TYK19589.1 (putative protein phosphatase 2C 76 [Cucumis melo var. makuwa])

HSP 1 Score: 672.5 bits (1734), Expect = 1.9e-189
Identity = 331/351 (94.30%), Postives = 342/351 (97.44%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRR+QI  +WRSLP+NLGF HTWKRE+RTSLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRIQINNIWRSLPSNLGFVHTWKREYRTSLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENR  IVD+HPEKDEDGGY SGGWKS+DG+LSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRRPIVDKHPEKDEDGGYTSGGWKSEDGRLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFL DTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLTDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSR IISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRTIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKDIELLV+ASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDIELLVVASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRD 352
           AVLVAGKE+EPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGPAADTR+
Sbjct: 301 AVLVAGKEDEPEAAARKLTEAAFTRGSADNITCIVVKFHHENAGPAADTRE 351

BLAST of MS000474 vs. NCBI nr
Match: XP_008439342.1 (PREDICTED: probable protein phosphatase 2C 76 [Cucumis melo] >XP_008439343.1 PREDICTED: probable protein phosphatase 2C 76 [Cucumis melo] >XP_008439344.1 PREDICTED: probable protein phosphatase 2C 76 [Cucumis melo] >XP_008439345.1 PREDICTED: probable protein phosphatase 2C 76 [Cucumis melo] >KAA0050431.1 putative protein phosphatase 2C 76 [Cucumis melo var. makuwa])

HSP 1 Score: 670.2 bits (1728), Expect = 9.5e-189
Identity = 330/351 (94.02%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRR+QI  +WRSLP+NLGF HTWKRE+R SLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRIQINNIWRSLPSNLGFVHTWKREYRISLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENR  IVD+HPEKDEDGGY SGGWKS+DG+LSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRRPIVDKHPEKDEDGGYTSGGWKSEDGRLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFL DTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLTDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSR IISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRTIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKDIELLV+ASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDIELLVVASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRD 352
           AVLVAGKE+EPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGPAADTR+
Sbjct: 301 AVLVAGKEDEPEAAARKLTEAAFTRGSADNITCIVVKFHHENAGPAADTRE 351

BLAST of MS000474 vs. NCBI nr
Match: XP_011651412.1 (probable protein phosphatase 2C 76 [Cucumis sativus] >XP_031739065.1 probable protein phosphatase 2C 76 [Cucumis sativus] >XP_031739066.1 probable protein phosphatase 2C 76 [Cucumis sativus] >KAE8650646.1 hypothetical protein Csa_010826 [Cucumis sativus])

HSP 1 Score: 667.9 bits (1722), Expect = 4.7e-188
Identity = 329/348 (94.54%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRRVQI  +WRSLP+NLGF HTWKRE+RTSLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRVQISNIWRSLPSNLGFVHTWKREYRTSLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENRG IVD+HPEKDEDGGY SGGWKS+DG+LSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRGPIVDKHPEKDEDGGYTSGGWKSEDGRLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFL DTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLTDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSR IISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRTIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKDIELLV+ASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDIELLVVASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAAD 349
           AVLVAGKE+EPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGP A+
Sbjct: 301 AVLVAGKEDEPEAAARKLTEAAFTRGSADNITCIVVKFHHENAGPVAE 348

BLAST of MS000474 vs. NCBI nr
Match: XP_038903569.1 (probable protein phosphatase 2C 76 isoform X1 [Benincasa hispida])

HSP 1 Score: 665.2 bits (1715), Expect = 3.1e-187
Identity = 327/351 (93.16%), Postives = 338/351 (96.30%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRN IAQAGHIGIINRRVQI  +WRSLP+NLG  HTWKRE+RTSLMMMVDTGAS
Sbjct: 1   MVCSYNIRNAIAQAGHIGIINRRVQINNIWRSLPSNLGSVHTWKREYRTSLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENRG +VD+HPEKDEDGGY SGGWKS+DGKLSWGYSSFRGKRATMEDFFDIKMSKV+GQT
Sbjct: 61  ENRGPVVDKHPEKDEDGGYTSGGWKSEDGKLSWGYSSFRGKRATMEDFFDIKMSKVNGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKF MDTKLAISETYQQTDA+FLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFFMDTKLAISETYQQTDADFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSR IISK GEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRTIISKAGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKDIELLVLASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDIELLVLASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRD 352
           AV VAGKE+EPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGPA DTR+
Sbjct: 301 AVSVAGKEDEPEAAARKLTEAAFTRGSADNITCIVVKFHHDNAGPAVDTRE 351

BLAST of MS000474 vs. ExPASy Swiss-Prot
Match: Q94AT1 (Probable protein phosphatase 2C 76 OS=Arabidopsis thaliana OX=3702 GN=At5g53140 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 1.7e-135
Identity = 247/349 (70.77%), Postives = 285/349 (81.66%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAG-HIGIINR-RVQIKYLWRSLPAN-------LGFAHTWKREFRTSL 60
           MVCS  IR+ I QAG  IG++ + R Q  ++ ++L          +GF  T    FRTS 
Sbjct: 1   MVCSSFIRSFIVQAGCRIGVLAQGRHQFIHIKKTLSVGFGFRTSVIGFRTTSGIGFRTSA 60

Query: 61  MMMVDTGASENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDI 120
            MMVDT A E R  +VD  PEK +DGGY  GGWK+DDG LS GY SFRGKR+TMEDF+DI
Sbjct: 61  KMMVDTSAGEKRISLVDMPPEKVDDGGYIGGGWKNDDGSLSCGYCSFRGKRSTMEDFYDI 120

Query: 121 KMSKVDGQTVCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAE 180
           K S ++GQ VC+FGIFDGHGGSRAAE+LK+HLF NLMKHP+FL DTKLA++ETY+QTD  
Sbjct: 121 KASTIEGQAVCMFGIFDGHGGSRAAEYLKEHLFNNLMKHPQFLTDTKLALNETYKQTDVA 180

Query: 181 FLNSEKDTLRDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERR 240
           FL SEKDT RDDGSTAS A+LVGNHLYVANVGDSR I+SK G+AI LS+DHKPNR+DER+
Sbjct: 181 FLESEKDTYRDDGSTASAAVLVGNHLYVANVGDSRTIVSKAGKAIALSDDHKPNRSDERK 240

Query: 241 RIENAGGVVMWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDG 300
           RIE+AGGV+MWAGTWRVGGVLAMSRAFGN+MLKQFVVA+PEIQDLE+D + ELLVLASDG
Sbjct: 241 RIESAGGVIMWAGTWRVGGVLAMSRAFGNRMLKQFVVAEPEIQDLEIDHEAELLVLASDG 300

Query: 301 LWDVVRNEDAVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHH 341
           LWDVV NEDAV +A  EEEPEAAARKLT+ AF+RGSADNITCIVVKF H
Sbjct: 301 LWDVVPNEDAVALAQSEEEPEAAARKLTDTAFSRGSADNITCIVVKFRH 349

BLAST of MS000474 vs. ExPASy Swiss-Prot
Match: Q6L5C4 (Probable protein phosphatase 2C 52 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0587100 PE=2 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.3e-119
Identity = 213/285 (74.74%), Postives = 251/285 (88.07%), Query Frame = 0

Query: 68  DQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIF 127
           ++  E+ ++ GY SGGWKS+DG LS GYSSFRGKRA+MEDF+DIK SK+D + + LFGIF
Sbjct: 205 EKERERVDEVGYMSGGWKSEDGFLSCGYSSFRGKRASMEDFYDIKSSKIDDKQISLFGIF 264

Query: 128 DGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGSTA 187
           DGHGGSRAAE+LK+HLFENLMKHP+F+ +TKLAISETY++TD+EFL+SE  T RDDGSTA
Sbjct: 265 DGHGGSRAAEYLKEHLFENLMKHPEFMTNTKLAISETYKKTDSEFLDSESHTHRDDGSTA 324

Query: 188 STALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWR 247
           STA+LVGNHLYVANVGDSRA+ISK G+AI LSEDHKPNR+DER+RIE+AGGVVMWAGTWR
Sbjct: 325 STAVLVGNHLYVANVGDSRAVISKAGKAIALSEDHKPNRSDERKRIESAGGVVMWAGTWR 384

Query: 248 VGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGK 307
           VGGVLAMSRAFGN++LKQFVVADPEIQ+ E+D ++E L+LASDGLWDVV NEDAV +   
Sbjct: 385 VGGVLAMSRAFGNRLLKQFVVADPEIQEQEIDDELEFLILASDGLWDVVPNEDAVSLVKI 444

Query: 308 EEEPEAAARKLTEAAFTRGSADNITCIVVKFHH----GNAGPAAD 349
           EEEPEAAARKLTE AF+RGS DNITCIVVKF H    G++ P +D
Sbjct: 445 EEEPEAAARKLTETAFSRGSGDNITCIVVKFQHDKMDGDSSPTSD 489

BLAST of MS000474 vs. ExPASy Swiss-Prot
Match: Q6EN45 (Probable protein phosphatase 2C member 13, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PP2C13 PE=2 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 5.5e-115
Identity = 208/312 (66.67%), Postives = 255/312 (81.73%), Query Frame = 0

Query: 41  HTWKREFRTSLMMMVDTGASENRGLIVDQHPEK---------DEDG---GYASGGWKSDD 100
           H+  R  R +  MM+D  +S++        P++          +DG   GYASGGW  +D
Sbjct: 50  HSGWRGLRVAARMMLD--SSDSAAAAGQMQPQQRAAGAVACSAQDGGAAGYASGGWARED 109

Query: 101 GKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIFDGHGGSRAAEFLKDHLFENLM 160
           GKL  GYSSFRGKRATMEDF+D+K++++DGQ V LFG+FDGHGG RAAE+LK++LFENL+
Sbjct: 110 GKLKCGYSSFRGKRATMEDFYDVKLTEIDGQAVSLFGVFDGHGGPRAAEYLKENLFENLL 169

Query: 161 KHPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGSTASTALLVGNHLYVANVGDSRAI 220
           KHP+FL DTKLAISETYQ+TD +FL SE +  RDDGSTASTA+LVG HLYVANVGDSRA+
Sbjct: 170 KHPEFLTDTKLAISETYQKTDTDFLESESNAFRDDGSTASTAVLVGGHLYVANVGDSRAV 229

Query: 221 ISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWRVGGVLAMSRAFGNKMLKQFVV 280
           +SK G+A+ LSEDHKPNR+DER+RIENAGGVV+WAGTWRVGGVLAMSRAFGN++LK FVV
Sbjct: 230 VSKAGKAMALSEDHKPNRSDERKRIENAGGVVIWAGTWRVGGVLAMSRAFGNRLLKPFVV 289

Query: 281 ADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGKEEEPEAAARKLTEAAFTRGSA 340
           A+PEIQ+  V++D+E LVLASDGLWDVV NE+AV +A  E+ PE+ ARKLTE A++RGSA
Sbjct: 290 AEPEIQEELVNEDLECLVLASDGLWDVVENEEAVSLAKTEDLPESVARKLTEIAYSRGSA 349

BLAST of MS000474 vs. ExPASy Swiss-Prot
Match: Q0JL75 (Probable protein phosphatase 2C 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0618200 PE=2 SV=2)

HSP 1 Score: 392.5 bits (1007), Expect = 5.0e-108
Identity = 190/269 (70.63%), Postives = 224/269 (83.27%), Query Frame = 0

Query: 72  EKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIFDGHG 131
           ++D++  Y  GGW SDDG++S GYSSFRG+RA MEDF+DIK SKVD   + LFGIFDGHG
Sbjct: 95  DEDDEARYICGGWTSDDGRMSCGYSSFRGRRANMEDFYDIKSSKVDDNQINLFGIFDGHG 154

Query: 132 GSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGSTASTAL 191
           GS AAE LK HLFENL+KHP F+ DTK AISETY++TD++FL++E +  R+DGSTASTA+
Sbjct: 155 GSHAAEHLKKHLFENLLKHPSFITDTKSAISETYRKTDSDFLDAETNINREDGSTASTAI 214

Query: 192 LVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWRVGGV 251
            VGNH+YVANVGDSR ++SK G+AI LS DHKPNR DER+RIENAGGVV W+GTWRVGGV
Sbjct: 215 FVGNHIYVANVGDSRTVMSKAGKAIALSSDHKPNRKDERKRIENAGGVVTWSGTWRVGGV 274

Query: 252 LAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGKEEEP 311
           LAMSRAFGN+ LK+FVVA+PE+Q+ E+D D+E L+LASDGLWDVV NE AV     EE P
Sbjct: 275 LAMSRAFGNRFLKRFVVAEPEVQEQEIDDDLEFLILASDGLWDVVSNEHAVAFVKAEEGP 334

Query: 312 EAAARKLTEAAFTRGSADNITCIVVKFHH 341
           EAAARKL E AF RGS DNITCIVVKF H
Sbjct: 335 EAAARKLAEIAFARGSTDNITCIVVKFLH 363

BLAST of MS000474 vs. ExPASy Swiss-Prot
Match: Q0DBU3 (Probable protein phosphatase 2C 56 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0526800 PE=3 SV=2)

HSP 1 Score: 347.4 bits (890), Expect = 1.9e-94
Identity = 168/270 (62.22%), Postives = 219/270 (81.11%), Query Frame = 0

Query: 75  EDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIFDGHGGSR 134
           +  G+ +GG  S+DG+LSW YSSF+G+R +MED F IKM+ ++ QTV LFG+FDGHGGS 
Sbjct: 79  QSNGWITGGSASEDGRLSWDYSSFKGRRPSMEDRFSIKMTTINEQTVSLFGVFDGHGGSL 138

Query: 135 AAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNS-EKDTLRDDGSTASTALLV 194
           AAE+LK+HLFENL+ HP+ L DTKLAIS+T+ +TDA+FL S   +  RDDGSTA TA+LV
Sbjct: 139 AAEYLKEHLFENLVNHPELLRDTKLAISQTFLKTDADFLESVSSNPFRDDGSTAVTAILV 198

Query: 195 GNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWRVGGVLA 254
           GNHLYV NVGDSR +  K G+A+PLSEDHKPNR DE++RIE+AGG+V++  TWRV G+LA
Sbjct: 199 GNHLYVGNVGDSRVVALKAGKAVPLSEDHKPNRKDEQKRIEDAGGIVVFDDTWRVNGLLA 258

Query: 255 MSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGKEEEPEA 314
           MSRAFGN+ LK +V A+P+IQ+  VD+ +E L+LA+DGLWDV+RNEDAV +   ++ P+A
Sbjct: 259 MSRAFGNRALKHYVKAEPDIQEKVVDESLEYLILATDGLWDVMRNEDAVSLLKAQDGPKA 318

Query: 315 AARKLTEAAFTRGSADNITCIVVKFHHGNA 344
           AA KLTE A +R + DNITCIV++FHHG +
Sbjct: 319 AAMKLTEVAHSRLTLDNITCIVLQFHHGKS 348

BLAST of MS000474 vs. ExPASy TrEMBL
Match: A0A6J1CGL3 (Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111011481 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 4.0e-201
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD 354
           AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD
Sbjct: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD 353

BLAST of MS000474 vs. ExPASy TrEMBL
Match: A0A5D3D7P7 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1274G00390 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 9.3e-190
Identity = 331/351 (94.30%), Postives = 342/351 (97.44%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRR+QI  +WRSLP+NLGF HTWKRE+RTSLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRIQINNIWRSLPSNLGFVHTWKREYRTSLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENR  IVD+HPEKDEDGGY SGGWKS+DG+LSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRRPIVDKHPEKDEDGGYTSGGWKSEDGRLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFL DTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLTDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSR IISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRTIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKDIELLV+ASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDIELLVVASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRD 352
           AVLVAGKE+EPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGPAADTR+
Sbjct: 301 AVLVAGKEDEPEAAARKLTEAAFTRGSADNITCIVVKFHHENAGPAADTRE 351

BLAST of MS000474 vs. ExPASy TrEMBL
Match: A0A1S3AZ87 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103484157 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 4.6e-189
Identity = 330/351 (94.02%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRR+QI  +WRSLP+NLGF HTWKRE+R SLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRIQINNIWRSLPSNLGFVHTWKREYRISLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENR  IVD+HPEKDEDGGY SGGWKS+DG+LSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRRPIVDKHPEKDEDGGYTSGGWKSEDGRLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFL DTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLTDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSR IISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRTIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKDIELLV+ASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDIELLVVASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRD 352
           AVLVAGKE+EPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGPAADTR+
Sbjct: 301 AVLVAGKEDEPEAAARKLTEAAFTRGSADNITCIVVKFHHENAGPAADTRE 351

BLAST of MS000474 vs. ExPASy TrEMBL
Match: A0A5A7UA70 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00190 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 4.6e-189
Identity = 330/351 (94.02%), Postives = 341/351 (97.15%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVIAQAGHIGIINRR+QI  +WRSLP+NLGF HTWKRE+R SLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVIAQAGHIGIINRRIQINNIWRSLPSNLGFVHTWKREYRISLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENR  IVD+HPEKDEDGGY SGGWKS+DG+LSWGYSSFRGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRRPIVDKHPEKDEDGGYTSGGWKSEDGRLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFL DTKLAISETYQQTDAEFLNSEKDTL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLTDTKLAISETYQQTDAEFLNSEKDTL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSR IISKGGEAIPLSEDHKPNRTDERRRIENAGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRTIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKDIELLV+ASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDIELLVVASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRD 352
           AVLVAGKE+EPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGPAADTR+
Sbjct: 301 AVLVAGKEDEPEAAARKLTEAAFTRGSADNITCIVVKFHHENAGPAADTRE 351

BLAST of MS000474 vs. ExPASy TrEMBL
Match: A0A6J1HU07 (Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111467783 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 4.3e-187
Identity = 325/353 (92.07%), Postives = 342/353 (96.88%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAGHIGIINRRVQIKYLWRSLPANLGFAHTWKREFRTSLMMMVDTGAS 60
           MVCSYNIRNVI+ AGH+GIINRRVQI  +WRSLP+NLGF HTWKRE+RTSLMMMVDTGAS
Sbjct: 1   MVCSYNIRNVISWAGHVGIINRRVQINNIWRSLPSNLGFVHTWKREYRTSLMMMVDTGAS 60

Query: 61  ENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQT 120
           ENR  +VD+HPEK+EDGGYASGGWKSDDGKLSWGYSS RGKRATMEDFFDIKMSKVDGQT
Sbjct: 61  ENRRPVVDKHPEKNEDGGYASGGWKSDDGKLSWGYSSIRGKRATMEDFFDIKMSKVDGQT 120

Query: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTL 180
           VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTK+AISETYQ+TDAEFL+ EK+TL
Sbjct: 121 VCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKVAISETYQRTDAEFLSLEKETL 180

Query: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVV 240
           RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIE AGGVV
Sbjct: 181 RDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIEKAGGVV 240

Query: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNED 300
           MWAGTWRVGGVLAMSRAFGNKMLKQFVVADP+IQDLEVDKD+ELL+LASDGLWDVVRNED
Sbjct: 241 MWAGTWRVGGVLAMSRAFGNKMLKQFVVADPDIQDLEVDKDVELLILASDGLWDVVRNED 300

Query: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAGPAADTRDAD 354
           AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHH NAGPAA+TR+ D
Sbjct: 301 AVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHDNAGPAAETREID 353

BLAST of MS000474 vs. TAIR 10
Match: AT5G53140.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.2e-136
Identity = 247/349 (70.77%), Postives = 285/349 (81.66%), Query Frame = 0

Query: 1   MVCSYNIRNVIAQAG-HIGIINR-RVQIKYLWRSLPAN-------LGFAHTWKREFRTSL 60
           MVCS  IR+ I QAG  IG++ + R Q  ++ ++L          +GF  T    FRTS 
Sbjct: 1   MVCSSFIRSFIVQAGCRIGVLAQGRHQFIHIKKTLSVGFGFRTSVIGFRTTSGIGFRTSA 60

Query: 61  MMMVDTGASENRGLIVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDI 120
            MMVDT A E R  +VD  PEK +DGGY  GGWK+DDG LS GY SFRGKR+TMEDF+DI
Sbjct: 61  KMMVDTSAGEKRISLVDMPPEKVDDGGYIGGGWKNDDGSLSCGYCSFRGKRSTMEDFYDI 120

Query: 121 KMSKVDGQTVCLFGIFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAE 180
           K S ++GQ VC+FGIFDGHGGSRAAE+LK+HLF NLMKHP+FL DTKLA++ETY+QTD  
Sbjct: 121 KASTIEGQAVCMFGIFDGHGGSRAAEYLKEHLFNNLMKHPQFLTDTKLALNETYKQTDVA 180

Query: 181 FLNSEKDTLRDDGSTASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERR 240
           FL SEKDT RDDGSTAS A+LVGNHLYVANVGDSR I+SK G+AI LS+DHKPNR+DER+
Sbjct: 181 FLESEKDTYRDDGSTASAAVLVGNHLYVANVGDSRTIVSKAGKAIALSDDHKPNRSDERK 240

Query: 241 RIENAGGVVMWAGTWRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDG 300
           RIE+AGGV+MWAGTWRVGGVLAMSRAFGN+MLKQFVVA+PEIQDLE+D + ELLVLASDG
Sbjct: 241 RIESAGGVIMWAGTWRVGGVLAMSRAFGNRMLKQFVVAEPEIQDLEIDHEAELLVLASDG 300

Query: 301 LWDVVRNEDAVLVAGKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHH 341
           LWDVV NEDAV +A  EEEPEAAARKLT+ AF+RGSADNITCIVVKF H
Sbjct: 301 LWDVVPNEDAVALAQSEEEPEAAARKLTDTAFSRGSADNITCIVVKFRH 349

BLAST of MS000474 vs. TAIR 10
Match: AT4G31750.1 (HOPW1-1-interacting 2 )

HSP 1 Score: 340.1 bits (871), Expect = 2.1e-93
Identity = 166/279 (59.50%), Postives = 218/279 (78.14%), Query Frame = 0

Query: 66  IVDQHPEKDEDGGYASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFG 125
           ++    +   D G  SGG  S +GK S+GY+S  GKR++MEDF++ ++  V+G+ V LFG
Sbjct: 7   VLSSSSQVHSDDGPVSGGGLSQNGKFSYGYASSPGKRSSMEDFYETRIDGVEGEIVGLFG 66

Query: 126 IFDGHGGSRAAEFLKDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGS 185
           +FDGHGG+RAAE++K +LF NL++HPKF+ DT  AI++ Y QTD+EFL SE    RD GS
Sbjct: 67  VFDGHGGARAAEYVKQNLFSNLIRHPKFISDTTAAIADAYNQTDSEFLKSENSQNRDAGS 126

Query: 186 TASTALLVGNHLYVANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGT 245
           TASTA+LVG+ L VANVGDSRA+I +GG AI +S DHKP+++DER+RIE+AGG VMWAGT
Sbjct: 127 TASTAILVGDRLLVANVGDSRAVICRGGNAIAVSRDHKPDQSDERQRIEDAGGFVMWAGT 186

Query: 246 WRVGGVLAMSRAFGNKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVA 305
           WRVGGVLA+SRAFG+++LKQ+VVADPEIQ+ +VD  +E L+LASDGLWDVV NE+AV + 
Sbjct: 187 WRVGGVLAVSRAFGDRLLKQYVVADPEIQEEKVDSSLEFLILASDGLWDVVSNEEAVGMI 246

Query: 306 GKEEEPEAAARKLTEAAFTRGSADNITCIVVKFHHGNAG 345
              E+PE  A++L   A+ RGSADNITC+VV+F    AG
Sbjct: 247 KAIEDPEEGAKRLMMEAYQRGSADNITCVVVRFFSDQAG 285

BLAST of MS000474 vs. TAIR 10
Match: AT5G24940.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 339.7 bits (870), Expect = 2.7e-93
Identity = 164/259 (63.32%), Postives = 207/259 (79.92%), Query Frame = 0

Query: 80  ASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIFDGHGGSRAAEFL 139
           ASGG  S +GK S+GY+S  GKR++MEDFF+ ++  +DG+ V LFG+FDGHGGSRAAE++
Sbjct: 21  ASGGGLSQNGKFSYGYASSAGKRSSMEDFFETRIDGIDGEIVGLFGVFDGHGGSRAAEYV 80

Query: 140 KDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGSTASTALLVGNHLYV 199
           K HLF NL+ HPKF+ DTK AI++ Y  TD+E L SE    RD GSTASTA+LVG+ L V
Sbjct: 81  KRHLFSNLITHPKFISDTKSAIADAYTHTDSELLKSENSHTRDAGSTASTAILVGDRLLV 140

Query: 200 ANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWRVGGVLAMSRAFG 259
           ANVGDSRA+I +GG A  +S DHKP+++DER RIENAGG VMWAGTWRVGGVLA+SRAFG
Sbjct: 141 ANVGDSRAVICRGGNAFAVSRDHKPDQSDERERIENAGGFVMWAGTWRVGGVLAVSRAFG 200

Query: 260 NKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGKEEEPEAAARKLT 319
           +++LKQ+VVADPEIQ+ ++D  +E L+LASDGLWDV  NE+AV V  + E+PE + +KL 
Sbjct: 201 DRLLKQYVVADPEIQEEKIDDSLEFLILASDGLWDVFSNEEAVAVVKEVEDPEESTKKLV 260

Query: 320 EAAFTRGSADNITCIVVKF 339
             A  RGSADNITC+VV+F
Sbjct: 261 GEAIKRGSADNITCVVVRF 279

BLAST of MS000474 vs. TAIR 10
Match: AT5G10740.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 338.2 bits (866), Expect = 8.0e-93
Identity = 164/259 (63.32%), Postives = 210/259 (81.08%), Query Frame = 0

Query: 80  ASGGWKSDDGKLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIFDGHGGSRAAEFL 139
           ASGG  S +GK S+GY+S  GKR++MEDFF+ ++  ++G+ V LFG+FDGHGG+RAAE++
Sbjct: 21  ASGGGLSQNGKFSYGYASSAGKRSSMEDFFETRIDGINGEIVGLFGVFDGHGGARAAEYV 80

Query: 140 KDHLFENLMKHPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGSTASTALLVGNHLYV 199
           K HLF NL+ HPKF+ DTK AI++ Y  TD+E L SE    RD GSTASTA+LVG+ L V
Sbjct: 81  KRHLFSNLITHPKFISDTKSAITDAYNHTDSELLKSENSHNRDAGSTASTAILVGDRLVV 140

Query: 200 ANVGDSRAIISKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWRVGGVLAMSRAFG 259
           ANVGDSRA+IS+GG+AI +S DHKP+++DER RIENAGG VMWAGTWRVGGVLA+SRAFG
Sbjct: 141 ANVGDSRAVISRGGKAIAVSRDHKPDQSDERERIENAGGFVMWAGTWRVGGVLAVSRAFG 200

Query: 260 NKMLKQFVVADPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGKEEEPEAAARKLT 319
           +++LKQ+VVADPEIQ+ ++D  +E L+LASDGLWDV  NE AV +  + E+PE +A+KL 
Sbjct: 201 DRLLKQYVVADPEIQEEKIDDTLEFLILASDGLWDVFSNEAAVAMVKEVEDPEDSAKKLV 260

Query: 320 EAAFTRGSADNITCIVVKF 339
             A  RGSADNITC+VV+F
Sbjct: 261 GEAIKRGSADNITCVVVRF 279

BLAST of MS000474 vs. TAIR 10
Match: AT1G43900.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 306.6 bits (784), Expect = 2.6e-83
Identity = 144/249 (57.83%), Postives = 197/249 (79.12%), Query Frame = 0

Query: 90  KLSWGYSSFRGKRATMEDFFDIKMSKVDGQTVCLFGIFDGHGGSRAAEFLKDHLFENLMK 149
           K S+GYSS +GKRATMED+F+ ++S V+GQ V  FG+FDGHGG+R AE+LK++LF+NL+ 
Sbjct: 121 KFSYGYSSLKGKRATMEDYFETRISDVNGQMVAFFGVFDGHGGARTAEYLKNNLFKNLVS 180

Query: 150 HPKFLMDTKLAISETYQQTDAEFLNSEKDTLRDDGSTASTALLVGNHLYVANVGDSRAII 209
           H  F+ DTK AI E ++QTD E+L  E    ++ GSTA+TA L+G+ L VANVGDSR + 
Sbjct: 181 HDDFISDTKKAIVEVFKQTDEEYLIEEAGQPKNAGSTAATAFLIGDKLIVANVGDSRVVA 240

Query: 210 SKGGEAIPLSEDHKPNRTDERRRIENAGGVVMWAGTWRVGGVLAMSRAFGNKMLKQFVVA 269
           S+ G A+PLS+DHKP+R+DER+RIE+AGG ++WAGTWRVGG+LA+SRAFG+K LK +V+A
Sbjct: 241 SRNGSAVPLSDDHKPDRSDERQRIEDAGGFIIWAGTWRVGGILAVSRAFGDKQLKPYVIA 300

Query: 270 DPEIQDLEVDKDIELLVLASDGLWDVVRNEDAVLVAGKEEEPEAAARKLTEAAFTRGSAD 329
           +PEIQ+ ++   +E +V+ASDGLW+V+ N+DAV +     + E AARKL +  + RGS D
Sbjct: 301 EPEIQEEDI-STLEFIVVASDGLWNVLSNKDAVAIVRDISDAETAARKLVQEGYARGSCD 360

Query: 330 NITCIVVKF 339
           NITCIVV+F
Sbjct: 361 NITCIVVRF 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140944.18.3e-201100.00probable protein phosphatase 2C 76 [Momordica charantia] >XP_022140945.1 probabl... [more]
TYK19589.11.9e-18994.30putative protein phosphatase 2C 76 [Cucumis melo var. makuwa][more]
XP_008439342.19.5e-18994.02PREDICTED: probable protein phosphatase 2C 76 [Cucumis melo] >XP_008439343.1 PRE... [more]
XP_011651412.14.7e-18894.54probable protein phosphatase 2C 76 [Cucumis sativus] >XP_031739065.1 probable pr... [more]
XP_038903569.13.1e-18793.16probable protein phosphatase 2C 76 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q94AT11.7e-13570.77Probable protein phosphatase 2C 76 OS=Arabidopsis thaliana OX=3702 GN=At5g53140 ... [more]
Q6L5C41.3e-11974.74Probable protein phosphatase 2C 52 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q6EN455.5e-11566.67Probable protein phosphatase 2C member 13, mitochondrial OS=Oryza sativa subsp. ... [more]
Q0JL755.0e-10870.63Probable protein phosphatase 2C 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q0DBU31.9e-9462.22Probable protein phosphatase 2C 56 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Match NameE-valueIdentityDescription
A0A6J1CGL34.0e-201100.00Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A5D3D7P79.3e-19094.30Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3AZ874.6e-18994.02Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103484157 PE=... [more]
A0A5A7UA704.6e-18994.02Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1HU074.3e-18792.07Protein-serine/threonine phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111467783... [more]
Match NameE-valueIdentityDescription
AT5G53140.11.2e-13670.77Protein phosphatase 2C family protein [more]
AT4G31750.12.1e-9359.50HOPW1-1-interacting 2 [more]
AT5G24940.12.7e-9363.32Protein phosphatase 2C family protein [more]
AT5G10740.18.0e-9363.32Protein phosphatase 2C family protein [more]
AT1G43900.12.6e-8357.83Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00331PP2C_SIG_2coord: 90..338
e-value: 0.0025
score: 9.6
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 82..336
e-value: 2.2E-89
score: 312.9
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 96..331
e-value: 4.4E-63
score: 213.3
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 92..338
score: 51.818501
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 94..338
e-value: 1.93791E-92
score: 274.974
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 84..342
e-value: 1.8E-88
score: 299.0
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 90..339
NoneNo IPR availablePANTHERPTHR47992:SF70PROTEIN PHOSPHATASE 2C 52-RELATEDcoord: 45..347
NoneNo IPR availablePANTHERPTHR47992ALPHABET, ISOFORM E-RELATEDcoord: 45..347
IPR000222PPM-type phosphatase, divalent cation bindingPROSITEPS01032PPM_1coord: 123..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000474.1MS000474.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0043169 cation binding
molecular_function GO:0016791 phosphatase activity