MS000473 (gene) Bitter gourd (TR) v1

Overview


Name	MS000473
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Trehalose 6-phosphate phosphatase
Location	scaffold44: 1943005 .. 1945107 (-)
RNA-Seq Expression	MS000473
Synteny	MS000473

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTATATTATTGTACAGAGAAAACACCCTTGTGGTTTGGAATCGTTTGAGAAGATGATGAAGGGAGCCAAAGGGAAGAAAATTGTTTTGTTTTTGGATTATGATGGAACCCTCTCTCCCATTGTTGATGATCCTGATCGTGCTTTCATGTCTCCTGAGGTAAATATTTTTTTTCCTAATTTTATTTTTTACATCGATATAATTAACTTTCCATACTTTCACCACCATTAATTATATTCAAATCTTAAAAATATTTAATGCCGTCATGCTTGGGATATTGGGATATTGGGATATACCCATATATATTACATAATATTCTTGTTTTTCATTTTTTTTTAAACTTCATTGAAGGAAAACCATTAATTTAATGGTGTTTTTTTTTCCTTTATTCTCTAAAATACCAAATTAGGAAAATAAAAAGTTTACTTTAAAGAGATAGGTGTCGTCAATTTGCTTTCCAAAGTTTTCATTTACTCTCTAGAATTTGAGTTTTGTTTGTCCTTATTTTACGAATTTTAAGTTGGTCAAACTTTCGTTATATTTTTTAACGCAATAATGAGGTGATATTTTGTATCTGGTTAATTGACCTTATTAGGGTTTAGGGTTTGAATAAAAGATAGAGGTATAATCAAGGGACAAAATTATATTAATGGGAAACTAAAACCCAAATTAAAGTAGGATGAAATTTACCCTTTAACCTAAAACATGACCATAGTTTTAATAAAAGTCAAAGAAATTGCGATCGTCTTTTTTTAAAATAAGTACATACGAGAGAAATAGAATATCTTAATATCTTAATTTTCGAAATTTTTTTTATAATGTTTGTGAAATAATAGACGATTTTTTTTGTTTTTTTGTTTTTTTTGAATGCAGATGAGAGCAGGTGTGAGAGAAGTGGCAAACTGCTTTCCAACAGCAATAATAAGCGGAAGGAGCCGTGACAAAGTATGAATTAATTAACATTTATATTTTCAATTGAACAAAATAAGCCGTAATTAATTAATTATTAGATAAAATAAAATATTGGTTTCAGGTGAAAGAATTCGTGAAGTTGAATAACGTGTATTATGCTGGGAGCCACGGAATGGACATTATGGCTCCAACATCCGACAATGAGGTTATTTTCTTGTGGTTTTATAACATTTAATTTCGACATGTCGACTTTAATTCAAATAAATAAATAAATAATGATATTGTGTACTGCATTTGCATTTGCAGGTGGAGGAGGTTGCGTATCAACCCGCCAAAAAGTTCCTGCCTGCAGTTCAACAGGTTATAATTAATTTGGCTTCTGTTTAAAAAATTATAAAATAAAAAAACAGTAATTAATTTGATATTTAATTATTTACACAGATAAAGGCCGTGTTGGAAGAAGAAATTACAGAAATAGAAGGTGCAGTGGTGGAGGATAATAGATTCTGTGTTTCTGTGCATTATCGACACGTCCGCGAACAGGTCACCAATTTTAAATTTTAAATTTTCCTCTCTCTCTCTCTATATATCTGCACGTCACGTGCATGTGCATGTGCACGTGCTTGAAGAAAAGTTTTTATATCTTTATTATTGACATGCAGGACCTGGAGAAATTGGAGGAGAAGGTAGAGTCTGTACTGGAAAACTATCCAGATTTCCATGTAACGCAGGGCAAAAAGGTAAATTCAATTTTTTTTTGCTCGTGATGTGACAATATGTGAATTGTGTATTAATTTGTATGAAAATTTTGAAATATATAGGTTATAGAAATAAGACCGACAATAAACTGGAACAAAGGGCATGCGGTGGAATATTATCTTCATATTCTTGGACATACTAATTCTAAGGATATAATTCCTGTGTATATTGGTGACGATCGAACCGATGAAGATGCATTCAAGGTATTTTTTATTTTTAAAGAAAATATTTGATGGTGGAAATTAATGGAATAAATAGAATTAATTTGTTTTGTAGGCAATACAAAGGAGAGGAGGAGAAGGGATTGGAATTCTTGTATCTTCCATTCCAAAGCAAACAAAAGCTTGCTACACTTTGAAGGATCCATCTCAAGTTTTAATTTTCTTGCAAAGCTTAGCAAGATGGAAGCGTGCTTCTTCAATCACAGACCCT

mRNA sequence

AATTATATTATTGTACAGAGAAAACACCCTTGTGGTTTGGAATCGTTTGAGAAGATGATGAAGGGAGCCAAAGGGAAGAAAATTGTTTTGTTTTTGGATTATGATGGAACCCTCTCTCCCATTGTTGATGATCCTGATCGTGCTTTCATGTCTCCTGAGATGAGAGCAGGTGTGAGAGAAGTGGCAAACTGCTTTCCAACAGCAATAATAAGCGGAAGGAGCCGTGACAAAGTGAAAGAATTCGTGAAGTTGAATAACGTGTATTATGCTGGGAGCCACGGAATGGACATTATGGCTCCAACATCCGACAATGAGGTGGAGGAGGTTGCGTATCAACCCGCCAAAAAGTTCCTGCCTGCAGTTCAACAGATAAAGGCCGTGTTGGAAGAAGAAATTACAGAAATAGAAGGTGCAGTGGTGGAGGATAATAGATTCTGTGTTTCTGTGCATTATCGACACGTCCGCGAACAGGACCTGGAGAAATTGGAGGAGAAGGTAGAGTCTGTACTGGAAAACTATCCAGATTTCCATGTAACGCAGGGCAAAAAGGTTATAGAAATAAGACCGACAATAAACTGGAACAAAGGGCATGCGGTGGAATATTATCTTCATATTCTTGGACATACTAATTCTAAGGATATAATTCCTGTGTATATTGGTGACGATCGAACCGATGAAGATGCATTCAAGGCAATACAAAGGAGAGGAGGAGAAGGGATTGGAATTCTTGTATCTTCCATTCCAAAGCAAACAAAAGCTTGCTACACTTTGAAGGATCCATCTCAAGTTTTAATTTTCTTGCAAAGCTTAGCAAGATGGAAGCGTGCTTCTTCAATCACAGACCCT

Coding sequence (CDS)

AATTATATTATTGTACAGAGAAAACACCCTTGTGGTTTGGAATCGTTTGAGAAGATGATGAAGGGAGCCAAAGGGAAGAAAATTGTTTTGTTTTTGGATTATGATGGAACCCTCTCTCCCATTGTTGATGATCCTGATCGTGCTTTCATGTCTCCTGAGATGAGAGCAGGTGTGAGAGAAGTGGCAAACTGCTTTCCAACAGCAATAATAAGCGGAAGGAGCCGTGACAAAGTGAAAGAATTCGTGAAGTTGAATAACGTGTATTATGCTGGGAGCCACGGAATGGACATTATGGCTCCAACATCCGACAATGAGGTGGAGGAGGTTGCGTATCAACCCGCCAAAAAGTTCCTGCCTGCAGTTCAACAGATAAAGGCCGTGTTGGAAGAAGAAATTACAGAAATAGAAGGTGCAGTGGTGGAGGATAATAGATTCTGTGTTTCTGTGCATTATCGACACGTCCGCGAACAGGACCTGGAGAAATTGGAGGAGAAGGTAGAGTCTGTACTGGAAAACTATCCAGATTTCCATGTAACGCAGGGCAAAAAGGTTATAGAAATAAGACCGACAATAAACTGGAACAAAGGGCATGCGGTGGAATATTATCTTCATATTCTTGGACATACTAATTCTAAGGATATAATTCCTGTGTATATTGGTGACGATCGAACCGATGAAGATGCATTCAAGGCAATACAAAGGAGAGGAGGAGAAGGGATTGGAATTCTTGTATCTTCCATTCCAAAGCAAACAAAAGCTTGCTACACTTTGAAGGATCCATCTCAAGTTTTAATTTTCTTGCAAAGCTTAGCAAGATGGAAGCGTGCTTCTTCAATCACAGACCCT

Protein sequence

NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRASSITDP

Homology

[Close]

BLAST of MS000473 vs. NCBI nr
Match: XP_022140946.1 (probable trehalose-phosphate phosphatase D [Momordica charantia])

HSP 1 Score: 562.8 bits (1449), Expect = 1.7e-156
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0


Query: 7   RKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFP 66
           RKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFP
Sbjct: 24  RKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFP 83

Query: 67  TAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKA 126
           TAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKA
Sbjct: 84  TAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKA 143

Query: 127 VLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIE 186
           VLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIE
Sbjct: 144 VLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIE 203

Query: 187 IRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSS 246
           IRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSS
Sbjct: 204 IRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSS 263

Query: 247 IPKQTKACYTLKDPSQVLIFLQSLARWKRASSITDP 283
           IPKQTKACYTLKDPSQVLIFLQSLARWKRASSITDP
Sbjct: 264 IPKQTKACYTLKDPSQVLIFLQSLARWKRASSITDP 299

[Close]

BLAST of MS000473 vs. NCBI nr
Match: XP_008439346.1 (PREDICTED: probable trehalose-phosphate phosphatase C [Cucumis melo])

HSP 1 Score: 449.9 bits (1156), Expect = 1.6e-122
Identity = 219/285 (76.84%), Postives = 249/285 (87.37%), Query Frame = 0


Query: 1   NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVRE 60
           NY    +KHPCGLESFE +MKG K KKIV+FLDYDGTLSPIVDDPDRAFMS EMR  VRE
Sbjct: 64  NYNSWLKKHPCGLESFEGIMKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVRE 123

Query: 61  VANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDI-MAPTSDNEVEEVAYQPAKKFLP 120
           VA CFPTAIISGRSRDKVKEFVKL+NV+YAGSHGMDI  APTSDNEV+ V++QPAKKFLP
Sbjct: 124 VAKCFPTAIISGRSRDKVKEFVKLSNVHYAGSHGMDITTAPTSDNEVDAVSFQPAKKFLP 183

Query: 121 AVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVT 180
            +QQI+ VLEEE  +IEGA+VEDNRFCVSVH+RHV E+DL+KLE+KVE+VLE YPDFH+T
Sbjct: 184 VIQQIREVLEEETRKIEGAMVEDNRFCVSVHFRHVHERDLDKLEKKVETVLERYPDFHIT 243

Query: 181 QGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEG 240
            GKKV+EIRPTINWNKGHA+EYYLH LGHTN  D++P+YIGDDRTDEDAFK IQ R G+G
Sbjct: 244 SGKKVMEIRPTINWNKGHAMEYYLHTLGHTNEDDVVPLYIGDDRTDEDAFKVIQMR-GQG 303

Query: 241 IGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKR--ASSITDP 283
           I ILVSSIPK T+A Y+LKDPSQVL FL  L RW++  +SSIT+P
Sbjct: 304 IAILVSSIPKNTRASYSLKDPSQVLAFLLRLVRWRKLSSSSITNP 347

[Close]

BLAST of MS000473 vs. NCBI nr
Match: XP_038906676.1 (probable trehalose-phosphate phosphatase C isoform X1 [Benincasa hispida])

HSP 1 Score: 449.9 bits (1156), Expect = 1.6e-122
Identity = 218/285 (76.49%), Postives = 253/285 (88.77%), Query Frame = 0


Query: 1   NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVRE 60
           NY    +KHPCGLESFE++MKG + KKIV+FLDYDGTLSPIVDDPDRAFMS EMR  VRE
Sbjct: 67  NYSSWLKKHPCGLESFERIMKGLQRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVRE 126

Query: 61  VANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMD-IMAPTSDNEVEEVAYQPAKKFLP 120
           VA CFPTAIISGRSRDKVKEFVKL+NV+YAGSHGMD IM PTSDNEV+EV++QPAK+FLP
Sbjct: 127 VAKCFPTAIISGRSRDKVKEFVKLSNVHYAGSHGMDIIMTPTSDNEVDEVSFQPAKEFLP 186

Query: 121 AVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVT 180
           A+QQI+ +LEEE  +IEGA+VEDNRFCVSVH+RHV E+DLEKLE+KV+S++   PDFH+T
Sbjct: 187 AIQQIRQILEEETRKIEGAMVEDNRFCVSVHFRHVHERDLEKLEKKVQSIIGKNPDFHIT 246

Query: 181 QGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEG 240
           QGKKV+EIRPTINWNKGHA+EYYLHILGHT+  D+IP+YIGDDRTDEDAFK I+ R G+G
Sbjct: 247 QGKKVMEIRPTINWNKGHAMEYYLHILGHTSIDDVIPLYIGDDRTDEDAFKVIEMR-GQG 306

Query: 241 IGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKR--ASSITDP 283
           I ILVSSIPK TKA Y+LKDPSQVL FL  LARW++  +SSIT+P
Sbjct: 307 IAILVSSIPKHTKASYSLKDPSQVLAFLLRLARWRKVSSSSITNP 350

[Close]

BLAST of MS000473 vs. NCBI nr
Match: KAA0050432.1 (putative trehalose-phosphate phosphatase C [Cucumis melo var. makuwa])

HSP 1 Score: 448.4 bits (1152), Expect = 4.7e-122
Identity = 216/285 (75.79%), Postives = 248/285 (87.02%), Query Frame = 0


Query: 1   NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVRE 60
           NY    +KHPCGLESFE +MKG K KKIV+FLDYDGTLSPIVDDPDRAFMS EMR  VRE
Sbjct: 29  NYNSWLKKHPCGLESFEGIMKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVRE 88

Query: 61  VANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDI-MAPTSDNEVEEVAYQPAKKFLP 120
           VA CFPTAIISGRSRDKVKEFVKL+NV+YAGSHGMDI  APTSDNEV+ V++QPAKKFLP
Sbjct: 89  VAKCFPTAIISGRSRDKVKEFVKLSNVHYAGSHGMDITTAPTSDNEVDAVSFQPAKKFLP 148

Query: 121 AVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVT 180
            +QQI+ VLEEE  +IEGA+VEDNRFCVSVH+RHV E+DL+KLE+KVE+VLE YPDFH+T
Sbjct: 149 VIQQIREVLEEETRKIEGAMVEDNRFCVSVHFRHVHERDLDKLEKKVETVLERYPDFHIT 208

Query: 181 QGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEG 240
            GKKV+EIRPTINWNKGHA+EYYLH LGHTN  D++P+YIGDDRTDEDAFK + +  G+G
Sbjct: 209 SGKKVMEIRPTINWNKGHAMEYYLHTLGHTNEDDVVPLYIGDDRTDEDAFKVVIQMRGQG 268

Query: 241 IGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKR--ASSITDP 283
           I ILVSSIPK T+A Y+LKDPSQVL FL  L RW++  +SSIT+P
Sbjct: 269 IAILVSSIPKNTRASYSLKDPSQVLAFLLRLVRWRKLSSSSITNP 313

[Close]

BLAST of MS000473 vs. NCBI nr
Match: KAE8650645.1 (hypothetical protein Csa_009958 [Cucumis sativus])

HSP 1 Score: 438.3 bits (1126), Expect = 4.9e-119
Identity = 215/279 (77.06%), Postives = 244/279 (87.46%), Query Frame = 0


Query: 1   NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVRE 60
           NY    + HP GLESF+ MMKG K KKIV+FLDYDGTLSPIVDDPDRAFMS EMR  VRE
Sbjct: 29  NYNSWLKTHPSGLESFDGMMKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVRE 88

Query: 61  VANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDI-MAPTSDNEVEEVAYQPAKKFLP 120
           VA CFPTAIISGRSRDKVKEFVKL+NV+YAGSHGMDI M PTSDNEV+ V++QPAKKFLP
Sbjct: 89  VAKCFPTAIISGRSRDKVKEFVKLSNVHYAGSHGMDITMTPTSDNEVDGVSFQPAKKFLP 148

Query: 121 AVQQIKAVLEEEIT-EIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHV 180
           A+QQI+ VLEEEIT +IEGA+VEDNRFCVSVH+RHV E+DLE L+ KVE+VLE YPDFH+
Sbjct: 149 AIQQIREVLEEEITRKIEGAMVEDNRFCVSVHFRHVHERDLENLKRKVETVLERYPDFHI 208

Query: 181 TQGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGE 240
           T GKKV+EIRPTINWNKGHA+EYYLH LGHTN  D++P+YIGDDRTDEDAFK IQ+R G+
Sbjct: 209 TLGKKVMEIRPTINWNKGHAMEYYLHTLGHTNVDDVVPLYIGDDRTDEDAFKVIQKR-GQ 268

Query: 241 GIGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRAS 278
           GI ILVSSIPK T+A Y+LKDP QVL FL  LARW+++S
Sbjct: 269 GIAILVSSIPKNTRASYSLKDPPQVLAFLLRLARWRKSS 306

[Close]

BLAST of MS000473 vs. ExPASy Swiss-Prot
Match: Q9C9S4 (Trehalose-phosphate phosphatase B OS=Arabidopsis thaliana OX=3702 GN=TPPB PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.3e-86
Identity = 152/272 (55.88%), Postives = 208/272 (76.47%), Query Frame = 0


Query: 8   KHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPT 67
           + P  L  F++++  AKGK+IV+FLDYDGTLSPIV+DPD+AF++ EMR  V++VA+ FPT
Sbjct: 98  RFPSALNMFDEIVNAAKGKQIVMFLDYDGTLSPIVEDPDKAFITHEMREVVKDVASNFPT 157

Query: 68  AIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDN----EVEEVAYQPAKKFLPAVQQ 127
           AI++GRS +KV+ FV++N +YYAGSHGMDI  PT++N      E V +QPA++FLP +++
Sbjct: 158 AIVTGRSIEKVRSFVQVNEIYYAGSHGMDIEGPTNENSNGQSNERVLFQPAREFLPMIEK 217

Query: 128 IKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKK 187
           +  +LEE+   I GA+VE+N+FC+SVH+R V E+    L E V+SVL +YP   +TQG+K
Sbjct: 218 VVNILEEKTKWIPGAMVENNKFCLSVHFRRVDEKRWPALAEVVKSVLIDYPKLKLTQGRK 277

Query: 188 VIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGIL 247
           V+EIRPTI W+KG A+ + L  LG+ NS D++PVYIGDDRTDEDAFK ++ R G+G GIL
Sbjct: 278 VLEIRPTIKWDKGQALNFLLKSLGYENSDDVVPVYIGDDRTDEDAFKVLRER-GQGFGIL 337

Query: 248 VSSIPKQTKACYTLKDPSQVLIFLQSLARWKR 276
           VS +PK T A Y+L+DPSQV  FL+ L  WKR
Sbjct: 338 VSKVPKDTNASYSLQDPSQVNKFLERLVEWKR 368

[Close]

BLAST of MS000473 vs. ExPASy Swiss-Prot
Match: Q67XC9 (Probable trehalose-phosphate phosphatase D OS=Arabidopsis thaliana OX=3702 GN=TPPD PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 4.8e-85
Identity = 153/274 (55.84%), Postives = 202/274 (73.72%), Query Frame = 0


Query: 8   KHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPT 67
           +HP  L  FE++ + +KGK+IV+FLDYDGTLSPIV++PDRA+MS EMR  V+ VA  FPT
Sbjct: 93  QHPSALTMFEEIAEASKGKQIVMFLDYDGTLSPIVENPDRAYMSEEMREAVKGVARYFPT 152

Query: 68  AIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEE----VAYQPAKKFLPAVQQ 127
           AI++GR RDKV+ FVKL  +YYAGSHGMDI  P+  N+  +    V +Q A +FLP + +
Sbjct: 153 AIVTGRCRDKVRRFVKLPGLYYAGSHGMDIKGPSKRNKHNKNNKGVLFQAANEFLPMIDK 212

Query: 128 IKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKK 187
           +   L E++ +IEGA VE+N+FCVSVHYR V ++D   + E V S+L  YP   +TQG+K
Sbjct: 213 VSKCLVEKMRDIEGANVENNKFCVSVHYRCVDQKDWGLVAEHVTSILSEYPKLRLTQGRK 272

Query: 188 VIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGIL 247
           V+EIRPTI W+KG A+E+ L  LG  NS D++P+YIGDDRTDEDAFK + R  G+G GIL
Sbjct: 273 VLEIRPTIKWDKGKALEFLLESLGFANSNDVLPIYIGDDRTDEDAFKVL-RNKGQGFGIL 332

Query: 248 VSSIPKQTKACYTLKDPSQVLIFLQSLARWKRAS 278
           VS IPK+T A Y+L++PS+V  FLQ L  WK+ S
Sbjct: 333 VSKIPKETSATYSLQEPSEVGEFLQRLVEWKQMS 365

[Close]

BLAST of MS000473 vs. ExPASy Swiss-Prot
Match: Q75WV3 (Probable trehalose-phosphate phosphatase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP1 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.1e-84
Identity = 155/276 (56.16%), Postives = 206/276 (74.64%), Query Frame = 0


Query: 8   KHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPT 67
           K P  L SF++++  A+GKKI +FLDYDGTLSPIVDDPD+A MSP MRA VR VA  FPT
Sbjct: 96  KCPSALASFKQIVASAQGKKIAVFLDYDGTLSPIVDDPDKAVMSPVMRAAVRNVAKYFPT 155

Query: 68  AIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNE------VEEVAYQPAKKFLPAV 127
           AI+SGRSR+KV EFVKL  +YYAGSHGMDIMAP++++E       +   +QPA  FLP +
Sbjct: 156 AIVSGRSRNKVFEFVKLKELYYAGSHGMDIMAPSANHEHSAEKSKQANLFQPAHDFLPMI 215

Query: 128 QQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQG 187
            ++   L + ++ IEGA VE+N+FCVSVHYR+V E+D + +   V  VLE +P   VT G
Sbjct: 216 DEVTKSLLQVVSGIEGATVENNKFCVSVHYRNVAEKDWKLVARLVNEVLEAFPRLKVTNG 275

Query: 188 KKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIG 247
           + V+E+RP I+W+KG AVE+ L  LG  +S+++IP+YIGDDRTDEDAFK +++R   G G
Sbjct: 276 RMVLEVRPVIDWDKGKAVEFLLQSLGLNDSENVIPIYIGDDRTDEDAFKVLRQRNC-GYG 335

Query: 248 ILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRAS 278
           ILVS +PK+T+A Y+L+DPS+V+ FL  L RWK+ S
Sbjct: 336 ILVSQVPKETEAFYSLRDPSEVMEFLNFLVRWKKHS 370

[Close]

BLAST of MS000473 vs. ExPASy Swiss-Prot
Match: Q6ZGP8 (Probable trehalose-phosphate phosphatase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP4 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.1e-84
Identity = 152/272 (55.88%), Postives = 200/272 (73.53%), Query Frame = 0


Query: 7   RKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFP 66
           RKHP  L  FE++   ++GKKIV+F+DYDGTLSPIV DPD A+MS  MRA VREVA  FP
Sbjct: 89  RKHPSALSKFEEIAAKSRGKKIVMFMDYDGTLSPIVADPDTAYMSDAMRAAVREVAKTFP 148

Query: 67  TAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKA 126
           TAI+SGR RDKV+ FV L+++YYAGSHGMDI  P+S+   E    QPA +FLP + ++  
Sbjct: 149 TAIVSGRCRDKVRNFVGLSDLYYAGSHGMDIKGPSSN--PESALCQPASEFLPMIDEVYK 208

Query: 127 VLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIE 186
            L E+     GA VE+N+FC+SVH+R V E+    L E+V++V++ YP   +TQG+KV+E
Sbjct: 209 TLVEKTKSTPGAKVENNKFCLSVHFRCVDEKRWNALGEQVKAVIKEYPKLKLTQGRKVLE 268

Query: 187 IRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSS 246
           IRP+I W+KG A+E+ L  LG  N  D++PVYIGDDRTDEDAFK +++R G+G+GILVS 
Sbjct: 269 IRPSIEWDKGKALEFLLESLGFANCGDVMPVYIGDDRTDEDAFKVLRKR-GQGLGILVSK 328

Query: 247 IPKQTKACYTLKDPSQVLIFLQSLARWKRASS 279
            PK T A Y+L+DP++V+ FL  L  WKR SS
Sbjct: 329 CPKDTNASYSLQDPTEVMEFLLRLVEWKRKSS 357

[Close]

BLAST of MS000473 vs. ExPASy Swiss-Prot
Match: Q9FWQ2 (Probable trehalose-phosphate phosphatase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP2 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.2e-83
Identity = 151/286 (52.80%), Postives = 206/286 (72.03%), Query Frame = 0


Query: 9   HPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPTA 68
           HP  L SFE ++  A+GK++ LFLDYDGTLSPIVD+P+ A MS EMR+ V+ VA+ FPTA
Sbjct: 98  HPSALTSFEDIVNLARGKRLALFLDYDGTLSPIVDNPENAVMSDEMRSAVKHVASLFPTA 157

Query: 69  IISGRSRDKVKEFVKLNNVYYAGSHGMDIMAP---------------TSDNEVEEV-AYQ 128
           IISGRSRDKV +FVKL  +YYAGSHGMDIM P               ++D+E +EV  +Q
Sbjct: 158 IISGRSRDKVFDFVKLTELYYAGSHGMDIMGPVRKSDSSGQHVECIRSTDSEGKEVNLFQ 217

Query: 129 PAKKFLPAVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLEN 188
           PA +FLP + ++   L E I +I+GA +EDN+FCVSVHYR+V   D  ++ ++V +VL+N
Sbjct: 218 PASEFLPMISEVYKKLSESIKDIDGARMEDNKFCVSVHYRNVAPHDYGEVHQRVTAVLKN 277

Query: 189 YPDFHVTQGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAI 248
           YP   +T G+KV+E+RP I+WNKG AVE+ L  LG    +D++P+Y+GDD+TDEDAFK +
Sbjct: 278 YPCLRLTHGRKVLEVRPVIDWNKGKAVEFLLESLGLCGKEDVLPIYVGDDKTDEDAFKVL 337

Query: 249 QRRGGEGIGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRASS 279
            +    G GILVSS+PK T A Y+++DP++V+ FL+ LA WK  S+
Sbjct: 338 -KANSIGFGILVSSVPKDTDAFYSVRDPAEVMEFLKKLASWKEEST 382

[Close]

BLAST of MS000473 vs. ExPASy TrEMBL
Match: A0A6J1CHI7 (Trehalose 6-phosphate phosphatase OS=Momordica charantia OX=3673 GN=LOC111011482 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 8.3e-157
Identity = 276/276 (100.00%), Postives = 276/276 (100.00%), Query Frame = 0


Query: 7   RKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFP 66
           RKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFP
Sbjct: 24  RKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFP 83

Query: 67  TAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKA 126
           TAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKA
Sbjct: 84  TAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPAVQQIKA 143

Query: 127 VLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIE 186
           VLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIE
Sbjct: 144 VLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIE 203

Query: 187 IRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSS 246
           IRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSS
Sbjct: 204 IRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSS 263

Query: 247 IPKQTKACYTLKDPSQVLIFLQSLARWKRASSITDP 283
           IPKQTKACYTLKDPSQVLIFLQSLARWKRASSITDP
Sbjct: 264 IPKQTKACYTLKDPSQVLIFLQSLARWKRASSITDP 299

[Close]

BLAST of MS000473 vs. ExPASy TrEMBL
Match: A0A1S3AY70 (Trehalose 6-phosphate phosphatase OS=Cucumis melo OX=3656 GN=LOC103484159 PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 7.8e-123
Identity = 219/285 (76.84%), Postives = 249/285 (87.37%), Query Frame = 0


Query: 1   NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVRE 60
           NY    +KHPCGLESFE +MKG K KKIV+FLDYDGTLSPIVDDPDRAFMS EMR  VRE
Sbjct: 64  NYNSWLKKHPCGLESFEGIMKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVRE 123

Query: 61  VANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDI-MAPTSDNEVEEVAYQPAKKFLP 120
           VA CFPTAIISGRSRDKVKEFVKL+NV+YAGSHGMDI  APTSDNEV+ V++QPAKKFLP
Sbjct: 124 VAKCFPTAIISGRSRDKVKEFVKLSNVHYAGSHGMDITTAPTSDNEVDAVSFQPAKKFLP 183

Query: 121 AVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVT 180
            +QQI+ VLEEE  +IEGA+VEDNRFCVSVH+RHV E+DL+KLE+KVE+VLE YPDFH+T
Sbjct: 184 VIQQIREVLEEETRKIEGAMVEDNRFCVSVHFRHVHERDLDKLEKKVETVLERYPDFHIT 243

Query: 181 QGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEG 240
            GKKV+EIRPTINWNKGHA+EYYLH LGHTN  D++P+YIGDDRTDEDAFK IQ R G+G
Sbjct: 244 SGKKVMEIRPTINWNKGHAMEYYLHTLGHTNEDDVVPLYIGDDRTDEDAFKVIQMR-GQG 303

Query: 241 IGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKR--ASSITDP 283
           I ILVSSIPK T+A Y+LKDPSQVL FL  L RW++  +SSIT+P
Sbjct: 304 IAILVSSIPKNTRASYSLKDPSQVLAFLLRLVRWRKLSSSSITNP 347

[Close]

BLAST of MS000473 vs. ExPASy TrEMBL
Match: A0A5A7U3L2 (Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00200 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.3e-122
Identity = 216/285 (75.79%), Postives = 248/285 (87.02%), Query Frame = 0


Query: 1   NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVRE 60
           NY    +KHPCGLESFE +MKG K KKIV+FLDYDGTLSPIVDDPDRAFMS EMR  VRE
Sbjct: 29  NYNSWLKKHPCGLESFEGIMKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVRE 88

Query: 61  VANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDI-MAPTSDNEVEEVAYQPAKKFLP 120
           VA CFPTAIISGRSRDKVKEFVKL+NV+YAGSHGMDI  APTSDNEV+ V++QPAKKFLP
Sbjct: 89  VAKCFPTAIISGRSRDKVKEFVKLSNVHYAGSHGMDITTAPTSDNEVDAVSFQPAKKFLP 148

Query: 121 AVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVT 180
            +QQI+ VLEEE  +IEGA+VEDNRFCVSVH+RHV E+DL+KLE+KVE+VLE YPDFH+T
Sbjct: 149 VIQQIREVLEEETRKIEGAMVEDNRFCVSVHFRHVHERDLDKLEKKVETVLERYPDFHIT 208

Query: 181 QGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEG 240
            GKKV+EIRPTINWNKGHA+EYYLH LGHTN  D++P+YIGDDRTDEDAFK + +  G+G
Sbjct: 209 SGKKVMEIRPTINWNKGHAMEYYLHTLGHTNEDDVVPLYIGDDRTDEDAFKVVIQMRGQG 268

Query: 241 IGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKR--ASSITDP 283
           I ILVSSIPK T+A Y+LKDPSQVL FL  L RW++  +SSIT+P
Sbjct: 269 IAILVSSIPKNTRASYSLKDPSQVLAFLLRLVRWRKLSSSSITNP 313

[Close]

BLAST of MS000473 vs. ExPASy TrEMBL
Match: A0A6J1EA53 (Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111430746 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.8e-117
Identity = 208/284 (73.24%), Postives = 241/284 (84.86%), Query Frame = 0


Query: 1   NYIIVQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVRE 60
           NY    +++P GLESFE +MK    KKIV+FLDYDGTLSPIVDDPDRAFMSPEMR  VRE
Sbjct: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120

Query: 61  VANCFPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVAYQPAKKFLPA 120
           VA CFPTAIISGRSR+KVKEFVKL N++YAGSHG+DIMAP SDNE   V+YQPAKKFLP 
Sbjct: 121 VAKCFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMAPNSDNEANGVSYQPAKKFLPE 180

Query: 121 VQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQ 180
           +QQIK  LEEE  +IEGA+VEDN FCVSVH+RHV E+DL KLE+KVE+V+ENYPDFHVTQ
Sbjct: 181 IQQIKRALEEESRKIEGALVEDNTFCVSVHFRHVHERDLGKLEKKVETVMENYPDFHVTQ 240

Query: 181 GKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGI 240
           GKKV+EIRPTINWNKGHA+EYYLH LGH ++ D++P+YIGDD+TDEDAFK IQR+ G+GI
Sbjct: 241 GKKVMEIRPTINWNKGHAMEYYLHTLGHNDTDDVVPLYIGDDQTDEDAFKVIQRK-GQGI 300

Query: 241 GILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRAS--SITDP 283
            ILV+SIPK T A Y+LKDPS+VL FL  LARW  ++  SIT P
Sbjct: 301 AILVASIPKHTNASYSLKDPSEVLAFLLRLARWSNSTSPSITTP 343

[Close]

BLAST of MS000473 vs. ExPASy TrEMBL
Match: A0A5D3D7P4 (Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1274G00380 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.3e-114
Identity = 203/266 (76.32%), Postives = 234/266 (87.97%), Query Frame = 0


Query: 20  MKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPTAIISGRSRDKVK 79
           MKG K KKIV+FLDYDGTLSPIVDDPDRAFMS EMR  VREVA CFPTAIISGRSRDKVK
Sbjct: 1   MKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVREVAKCFPTAIISGRSRDKVK 60

Query: 80  EFVKLNNVYYAGSHGMDI-MAPTSDNEVEEVAYQPAKKFLPAVQQIKAVLEEEITEIEGA 139
           EFVKL+NV+YAGSHGMDI  APTSDNEV+ V++QPAKKFLP +QQI+ VLEEE  ++EGA
Sbjct: 61  EFVKLSNVHYAGSHGMDITTAPTSDNEVDAVSFQPAKKFLPVIQQIREVLEEETRKVEGA 120

Query: 140 VVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKKVIEIRPTINWNKGHA 199
           +VEDNRFCVSVH+RHV E+DL+KLE+KVE+VLE YPDFH+T GKKV+EIRPTINWNKGHA
Sbjct: 121 MVEDNRFCVSVHFRHVHERDLDKLEKKVETVLERYPDFHITSGKKVMEIRPTINWNKGHA 180

Query: 200 VEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGILVSSIPKQTKACYTLK 259
           +EYYLH LGHTN  D++P+YIGDDRTDEDAFK + +  G+GI ILVSSIPK T+A Y+LK
Sbjct: 181 MEYYLHTLGHTNEDDVVPLYIGDDRTDEDAFKVVIQMRGQGIAILVSSIPKNTRASYSLK 240

Query: 260 DPSQVLIFLQSLARWKR--ASSITDP 283
           DPSQVL FL  L RW++  +SSIT+P
Sbjct: 241 DPSQVLAFLLRLVRWRKLSSSSITNP 266

[Close]

BLAST of MS000473 vs. TAIR 10
Match: AT1G78090.1 (trehalose-6-phosphate phosphatase )

HSP 1 Score: 319.3 bits (817), Expect = 3.1e-87
Identity = 152/272 (55.88%), Postives = 208/272 (76.47%), Query Frame = 0


Query: 8   KHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPT 67
           + P  L  F++++  AKGK+IV+FLDYDGTLSPIV+DPD+AF++ EMR  V++VA+ FPT
Sbjct: 98  RFPSALNMFDEIVNAAKGKQIVMFLDYDGTLSPIVEDPDKAFITHEMREVVKDVASNFPT 157

Query: 68  AIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDN----EVEEVAYQPAKKFLPAVQQ 127
           AI++GRS +KV+ FV++N +YYAGSHGMDI  PT++N      E V +QPA++FLP +++
Sbjct: 158 AIVTGRSIEKVRSFVQVNEIYYAGSHGMDIEGPTNENSNGQSNERVLFQPAREFLPMIEK 217

Query: 128 IKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKK 187
           +  +LEE+   I GA+VE+N+FC+SVH+R V E+    L E V+SVL +YP   +TQG+K
Sbjct: 218 VVNILEEKTKWIPGAMVENNKFCLSVHFRRVDEKRWPALAEVVKSVLIDYPKLKLTQGRK 277

Query: 188 VIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGIL 247
           V+EIRPTI W+KG A+ + L  LG+ NS D++PVYIGDDRTDEDAFK ++ R G+G GIL
Sbjct: 278 VLEIRPTIKWDKGQALNFLLKSLGYENSDDVVPVYIGDDRTDEDAFKVLRER-GQGFGIL 337

Query: 248 VSSIPKQTKACYTLKDPSQVLIFLQSLARWKR 276
           VS +PK T A Y+L+DPSQV  FL+ L  WKR
Sbjct: 338 VSKVPKDTNASYSLQDPSQVNKFLERLVEWKR 368

[Close]

BLAST of MS000473 vs. TAIR 10
Match: AT1G35910.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 315.8 bits (808), Expect = 3.4e-86
Identity = 153/274 (55.84%), Postives = 202/274 (73.72%), Query Frame = 0


Query: 8   KHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPT 67
           +HP  L  FE++ + +KGK+IV+FLDYDGTLSPIV++PDRA+MS EMR  V+ VA  FPT
Sbjct: 93  QHPSALTMFEEIAEASKGKQIVMFLDYDGTLSPIVENPDRAYMSEEMREAVKGVARYFPT 152

Query: 68  AIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEE----VAYQPAKKFLPAVQQ 127
           AI++GR RDKV+ FVKL  +YYAGSHGMDI  P+  N+  +    V +Q A +FLP + +
Sbjct: 153 AIVTGRCRDKVRRFVKLPGLYYAGSHGMDIKGPSKRNKHNKNNKGVLFQAANEFLPMIDK 212

Query: 128 IKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENYPDFHVTQGKK 187
           +   L E++ +IEGA VE+N+FCVSVHYR V ++D   + E V S+L  YP   +TQG+K
Sbjct: 213 VSKCLVEKMRDIEGANVENNKFCVSVHYRCVDQKDWGLVAEHVTSILSEYPKLRLTQGRK 272

Query: 188 VIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQRRGGEGIGIL 247
           V+EIRPTI W+KG A+E+ L  LG  NS D++P+YIGDDRTDEDAFK + R  G+G GIL
Sbjct: 273 VLEIRPTIKWDKGKALEFLLESLGFANSNDVLPIYIGDDRTDEDAFKVL-RNKGQGFGIL 332

Query: 248 VSSIPKQTKACYTLKDPSQVLIFLQSLARWKRAS 278
           VS IPK+T A Y+L++PS+V  FLQ L  WK+ S
Sbjct: 333 VSKIPKETSATYSLQEPSEVGEFLQRLVEWKQMS 365

[Close]

BLAST of MS000473 vs. TAIR 10
Match: AT5G51460.3 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.2e-84
Identity = 149/286 (52.10%), Postives = 202/286 (70.63%), Query Frame = 0


Query: 5   VQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANC 64
           +Q K+P  L SFEK+M  AKGK+I LFLDYDGTLSPIV++PD A+MS  MR+ V+ VA  
Sbjct: 98  MQLKYPSALTSFEKIMSFAKGKRIALFLDYDGTLSPIVEEPDCAYMSSAMRSAVQNVAKY 157

Query: 65  FPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVA-------------- 124
           FPTAIISGRSRDKV EFV L+ +YYAGSHGMDIM+P  ++   E +              
Sbjct: 158 FPTAIISGRSRDKVYEFVNLSELYYAGSHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNL 217

Query: 125 YQPAKKFLPAVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVL 184
           +QPA +FLP + ++   L E   +I+G  VEDN+FC+SVHYR+V E++   + + V+ V+
Sbjct: 218 FQPASEFLPMIDKVLCSLIESTKDIKGVKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVI 277

Query: 185 ENYPDFHVTQGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFK 244
             YP   +T G+KV+EIRP I+W+KG AV + L  LG  N +D++P+Y+GDDRTDEDAFK
Sbjct: 278 RTYPKLRLTHGRKVLEIRPVIDWDKGKAVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFK 337

Query: 245 AIQRRGGEGIGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRA 277
            ++     G GILVS++PK + A Y+L+DPS+V+ FL+SL  WKR+
Sbjct: 338 VLRDGPNHGYGILVSAVPKDSNAFYSLRDPSEVMEFLKSLVTWKRS 383

[Close]

BLAST of MS000473 vs. TAIR 10
Match: AT5G51460.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.2e-84
Identity = 149/286 (52.10%), Postives = 202/286 (70.63%), Query Frame = 0


Query: 5   VQRKHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANC 64
           +Q K+P  L SFEK+M  AKGK+I LFLDYDGTLSPIV++PD A+MS  MR+ V+ VA  
Sbjct: 98  MQLKYPSALTSFEKIMSFAKGKRIALFLDYDGTLSPIVEEPDCAYMSSAMRSAVQNVAKY 157

Query: 65  FPTAIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVA-------------- 124
           FPTAIISGRSRDKV EFV L+ +YYAGSHGMDIM+P  ++   E +              
Sbjct: 158 FPTAIISGRSRDKVYEFVNLSELYYAGSHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNL 217

Query: 125 YQPAKKFLPAVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVL 184
           +QPA +FLP + ++   L E   +I+G  VEDN+FC+SVHYR+V E++   + + V+ V+
Sbjct: 218 FQPASEFLPMIDKVLCSLIESTKDIKGVKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVI 277

Query: 185 ENYPDFHVTQGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFK 244
             YP   +T G+KV+EIRP I+W+KG AV + L  LG  N +D++P+Y+GDDRTDEDAFK
Sbjct: 278 RTYPKLRLTHGRKVLEIRPVIDWDKGKAVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFK 337

Query: 245 AIQRRGGEGIGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRA 277
            ++     G GILVS++PK + A Y+L+DPS+V+ FL+SL  WKR+
Sbjct: 338 VLRDGPNHGYGILVSAVPKDSNAFYSLRDPSEVMEFLKSLVTWKRS 383

[Close]

BLAST of MS000473 vs. TAIR 10
Match: AT5G51460.2 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.2e-83
Identity = 148/283 (52.30%), Postives = 200/283 (70.67%), Query Frame = 0


Query: 8   KHPCGLESFEKMMKGAKGKKIVLFLDYDGTLSPIVDDPDRAFMSPEMRAGVREVANCFPT 67
           K+P  L SFEK+M  AKGK+I LFLDYDGTLSPIV++PD A+MS  MR+ V+ VA  FPT
Sbjct: 100 KYPSALTSFEKIMSFAKGKRIALFLDYDGTLSPIVEEPDCAYMSSAMRSAVQNVAKYFPT 159

Query: 68  AIISGRSRDKVKEFVKLNNVYYAGSHGMDIMAPTSDNEVEEVA--------------YQP 127
           AIISGRSRDKV EFV L+ +YYAGSHGMDIM+P  ++   E +              +QP
Sbjct: 160 AIISGRSRDKVYEFVNLSELYYAGSHGMDIMSPAGESLNHEHSRTVSVYEQGKDVNLFQP 219

Query: 128 AKKFLPAVQQIKAVLEEEITEIEGAVVEDNRFCVSVHYRHVREQDLEKLEEKVESVLENY 187
           A +FLP + ++   L E   +I+G  VEDN+FC+SVHYR+V E++   + + V+ V+  Y
Sbjct: 220 ASEFLPMIDKVLCSLIESTKDIKGVKVEDNKFCISVHYRNVEEKNWTLVAQCVDDVIRTY 279

Query: 188 PDFHVTQGKKVIEIRPTINWNKGHAVEYYLHILGHTNSKDIIPVYIGDDRTDEDAFKAIQ 247
           P   +T G+KV+EIRP I+W+KG AV + L  LG  N +D++P+Y+GDDRTDEDAFK ++
Sbjct: 280 PKLRLTHGRKVLEIRPVIDWDKGKAVTFLLESLGLNNCEDVLPIYVGDDRTDEDAFKVLR 339

Query: 248 RRGGEGIGILVSSIPKQTKACYTLKDPSQVLIFLQSLARWKRA 277
                G GILVS++PK + A Y+L+DPS+V+ FL+SL  WKR+
Sbjct: 340 DGPNHGYGILVSAVPKDSNAFYSLRDPSEVMEFLKSLVTWKRS 382

The following BLAST results are available for this feature:

BLAST of MS000473 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140946.1	1.7e-156	100.00	probable trehalose-phosphate phosphatase D [Momordica charantia]	[more]
XP_008439346.1	1.6e-122	76.84	PREDICTED: probable trehalose-phosphate phosphatase C [Cucumis melo]	[more]
XP_038906676.1	1.6e-122	76.49	probable trehalose-phosphate phosphatase C isoform X1 [Benincasa hispida]	[more]
KAA0050432.1	4.7e-122	75.79	putative trehalose-phosphate phosphatase C [Cucumis melo var. makuwa]	[more]
KAE8650645.1	4.9e-119	77.06	hypothetical protein Csa_009958 [Cucumis sativus]	[more]

BLAST of MS000473 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9S4	4.3e-86	55.88	Trehalose-phosphate phosphatase B OS=Arabidopsis thaliana OX=3702 GN=TPPB PE=1 S...	[more]
Q67XC9	4.8e-85	55.84	Probable trehalose-phosphate phosphatase D OS=Arabidopsis thaliana OX=3702 GN=TP...	[more]
Q75WV3	1.1e-84	56.16	Probable trehalose-phosphate phosphatase 1 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q6ZGP8	3.1e-84	55.88	Probable trehalose-phosphate phosphatase 4 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q9FWQ2	1.2e-83	52.80	Probable trehalose-phosphate phosphatase 2 OS=Oryza sativa subsp. japonica OX=39...	[more]

BLAST of MS000473 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHI7	8.3e-157	100.00	Trehalose 6-phosphate phosphatase OS=Momordica charantia OX=3673 GN=LOC111011482...	[more]
A0A1S3AY70	7.8e-123	76.84	Trehalose 6-phosphate phosphatase OS=Cucumis melo OX=3656 GN=LOC103484159 PE=3 S...	[more]
A0A5A7U3L2	2.3e-122	75.79	Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A6J1EA53	3.8e-117	73.24	Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111430746 ...	[more]
A0A5D3D7P4	1.3e-114	76.32	Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]

BLAST of MS000473 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78090.1	3.1e-87	55.88	trehalose-6-phosphate phosphatase	[more]
AT1G35910.1	3.4e-86	55.84	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT5G51460.3	4.2e-84	52.10	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT5G51460.1	4.2e-84	52.10	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]
AT5G51460.2	1.2e-83	52.30	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 152..172
None	No IPR available	GENE3D	3.30.70.1020		coord: 114..190 e-value: 2.2E-51 score: 176.5
None	No IPR available	PANTHER	PTHR43768:SF24	TREHALOSE 6-PHOSPHATE PHOSPHATASE	coord: 8..278
None	No IPR available	CDD	cd01627	HAD_TPP	coord: 29..263 e-value: 1.74134E-66 score: 205.216
IPR003337	Trehalose-phosphatase	PFAM	PF02358	Trehalose_PPase	coord: 31..261 e-value: 5.1E-72 score: 241.8
IPR003337	Trehalose-phosphatase	TIGRFAM	TIGR00685	TIGR00685	coord: 25..271 e-value: 1.4E-56 score: 190.1
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 30..257 e-value: 2.2E-51 score: 176.5
IPR006379	HAD-superfamily hydrolase, subfamily IIB	TIGRFAM	TIGR01484	TIGR01484	coord: 30..235 e-value: 2.6E-20 score: 71.4
IPR044651	Trehalose 6-phosphate OTSB-like	PANTHER	PTHR43768	TREHALOSE 6-PHOSPHATE PHOSPHATASE	coord: 8..278
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 29..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000473.1	MS000473.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005992	trehalose biosynthetic process
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004805	trehalose-phosphatase activity