MS000470 (gene) Bitter gourd (TR) v1

Overview
NameMS000470
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionWRKY transcription factor 13
Locationscaffold44: 1923502 .. 1924796 (-)
RNA-Seq ExpressionMS000470
SyntenyMS000470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATATTGGGGAATGGGAGCAGAAGAATCTGAAAAATGAGCTACTTAGAGGGAAGGAATTGGCAAAGCAGCTCCAACTTTATCTCAATGTGCAACCCTCATCATCCATGCCTGCTTCTCCTTCTGAAGATGGTGAATTATTAGTTCAGAAAATCTTATCTTCTTATGAAAAGGCACTCTCATTTTTCAGCTCAACTGGGATCCAAATATCTGAATCTCCATCATCCCCTAATGGAAGTCCAAGGAGTGAGGACTCTGACCGTGAATTCAAGGACCAGGACCAGAGAATTAATGCTTCCCGCAAAAGGTAACTTTGAACAAAAGCTTATTTATGATCTGAACTGTTCTTAAAATCTCATAGTTTTGAGATATTTCCTGTTTTGTATGTTTGTAGGAACAGTCTTCCAACTTGGACACAGAAATTTCAAGTTAGCCCAGGGATGGCCCCTGAAGGCTCTCTTGATGATGGCTTTTGCTGGAGGAAATATGGTCAAAAAGACATTCTTGGTGCCAAACATCCAAGGTAAAATCTTTGGATCTCTTAACTATTCTAACATTTATTTGCCTATTTTGCTGTTTTAAAGCATTTTTGCAGCAAAATGGTTATATGGTTATGTGTCTGTTGCACACCAGCAGAGGCTATTACAGATGCACGCACAAGAACCTCCAAGGTTGTCTTGCAACCAAACAAGTTCAACGCTCCGACGATGATCCGACCGTCTTCGAAATCACGTACCGAGGAAACCATACTTGCAGCCAAGTCACCAACCTCAGTCCTCCATCAGCAGCACCCGAGTTTCAGCAACCAAACAGTAGCAATATTGATCCAAATTTGGTGCAAAAGCAGAGCCACTCTGTTCCTGACCAAAACCAGCAAAGTTCACCTGATGCTCTCTTGAACTCTTGGGCATCCCTCAGAGTCATAACTGAAAATCTTGACACTCATGAACCAACATTGTTTCCCGCTTTTAGTTATGATCCCACATCAAGTTACAAGGCTGCAGACCATGTCGAGTCGGCATCGATGACGGTCGGTGTGAACTTTGCCGAGTTTCCTCCTTCATTTTTATCCCCAACAACATCTTGTTCTGGGTTGAGCTATTTCTCTGCCTCCTCAGGTGGGTTGAGTGAAGGATTTGTTGGGAATCAGAAGTTGCAGGCAAACAAATCTGAGCTTTCTTTTCCCTCACCAACTTCAGCTCTAAACTCTCAAACTCTTGGCTCAGAGTTCCCATTTGGTGAGGTTGAAATGGAGCCAAGCTTCACATTTGACCACACAAATTTCTTCTCC

mRNA sequence

ATGGAGAATATTGGGGAATGGGAGCAGAAGAATCTGAAAAATGAGCTACTTAGAGGGAAGGAATTGGCAAAGCAGCTCCAACTTTATCTCAATGTGCAACCCTCATCATCCATGCCTGCTTCTCCTTCTGAAGATGGTGAATTATTAGTTCAGAAAATCTTATCTTCTTATGAAAAGGCACTCTCATTTTTCAGCTCAACTGGGATCCAAATATCTGAATCTCCATCATCCCCTAATGGAAGTCCAAGGAGTGAGGACTCTGACCGTGAATTCAAGGACCAGGACCAGAGAATTAATGCTTCCCGCAAAAGGAACAGTCTTCCAACTTGGACACAGAAATTTCAAGTTAGCCCAGGGATGGCCCCTGAAGGCTCTCTTGATGATGGCTTTTGCTGGAGGAAATATGGTCAAAAAGACATTCTTGGTGCCAAACATCCAAGCAGAGGCTATTACAGATGCACGCACAAGAACCTCCAAGGTTGTCTTGCAACCAAACAAGTTCAACGCTCCGACGATGATCCGACCGTCTTCGAAATCACGTACCGAGGAAACCATACTTGCAGCCAAGTCACCAACCTCAGTCCTCCATCAGCAGCACCCGAGTTTCAGCAACCAAACAGTAGCAATATTGATCCAAATTTGGTGCAAAAGCAGAGCCACTCTGTTCCTGACCAAAACCAGCAAAGTTCACCTGATGCTCTCTTGAACTCTTGGGCATCCCTCAGAGTCATAACTGAAAATCTTGACACTCATGAACCAACATTGTTTCCCGCTTTTAGTTATGATCCCACATCAAGTTACAAGGCTGCAGACCATGTCGAGTCGGCATCGATGACGGTCGGTGTGAACTTTGCCGAGTTTCCTCCTTCATTTTTATCCCCAACAACATCTTGTTCTGGGTTGAGCTATTTCTCTGCCTCCTCAGGTGGGTTGAGTGAAGGATTTGTTGGGAATCAGAAGTTGCAGGCAAACAAATCTGAGCTTTCTTTTCCCTCACCAACTTCAGCTCTAAACTCTCAAACTCTTGGCTCAGAGTTCCCATTTGGTGAGGTTGAAATGGAGCCAAGCTTCACATTTGACCACACAAATTTCTTCTCC

Coding sequence (CDS)

ATGGAGAATATTGGGGAATGGGAGCAGAAGAATCTGAAAAATGAGCTACTTAGAGGGAAGGAATTGGCAAAGCAGCTCCAACTTTATCTCAATGTGCAACCCTCATCATCCATGCCTGCTTCTCCTTCTGAAGATGGTGAATTATTAGTTCAGAAAATCTTATCTTCTTATGAAAAGGCACTCTCATTTTTCAGCTCAACTGGGATCCAAATATCTGAATCTCCATCATCCCCTAATGGAAGTCCAAGGAGTGAGGACTCTGACCGTGAATTCAAGGACCAGGACCAGAGAATTAATGCTTCCCGCAAAAGGAACAGTCTTCCAACTTGGACACAGAAATTTCAAGTTAGCCCAGGGATGGCCCCTGAAGGCTCTCTTGATGATGGCTTTTGCTGGAGGAAATATGGTCAAAAAGACATTCTTGGTGCCAAACATCCAAGCAGAGGCTATTACAGATGCACGCACAAGAACCTCCAAGGTTGTCTTGCAACCAAACAAGTTCAACGCTCCGACGATGATCCGACCGTCTTCGAAATCACGTACCGAGGAAACCATACTTGCAGCCAAGTCACCAACCTCAGTCCTCCATCAGCAGCACCCGAGTTTCAGCAACCAAACAGTAGCAATATTGATCCAAATTTGGTGCAAAAGCAGAGCCACTCTGTTCCTGACCAAAACCAGCAAAGTTCACCTGATGCTCTCTTGAACTCTTGGGCATCCCTCAGAGTCATAACTGAAAATCTTGACACTCATGAACCAACATTGTTTCCCGCTTTTAGTTATGATCCCACATCAAGTTACAAGGCTGCAGACCATGTCGAGTCGGCATCGATGACGGTCGGTGTGAACTTTGCCGAGTTTCCTCCTTCATTTTTATCCCCAACAACATCTTGTTCTGGGTTGAGCTATTTCTCTGCCTCCTCAGGTGGGTTGAGTGAAGGATTTGTTGGGAATCAGAAGTTGCAGGCAAACAAATCTGAGCTTTCTTTTCCCTCACCAACTTCAGCTCTAAACTCTCAAACTCTTGGCTCAGAGTTCCCATTTGGTGAGGTTGAAATGGAGCCAAGCTTCACATTTGACCACACAAATTTCTTCTCC

Protein sequence

MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASLRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGLSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDHTNFFS
Homology
BLAST of MS000470 vs. NCBI nr
Match: XP_022140883.1 (probable WRKY transcription factor 53 isoform X1 [Momordica charantia])

HSP 1 Score: 728.4 bits (1879), Expect = 3.1e-206
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 367
           HTNFFS
Sbjct: 361 HTNFFS 366

BLAST of MS000470 vs. NCBI nr
Match: XP_022140884.1 (probable WRKY transcription factor 53 isoform X2 [Momordica charantia])

HSP 1 Score: 721.8 bits (1862), Expect = 2.9e-204
Identity = 365/366 (99.73%), Postives = 365/366 (99.73%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHP RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHP-RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 367
           HTNFFS
Sbjct: 361 HTNFFS 365

BLAST of MS000470 vs. NCBI nr
Match: XP_022140885.1 (probable WRKY transcription factor 53 isoform X3 [Momordica charantia])

HSP 1 Score: 719.5 bits (1856), Expect = 1.4e-203
Identity = 364/366 (99.45%), Postives = 364/366 (99.45%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK  SLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK--SLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 367
           HTNFFS
Sbjct: 361 HTNFFS 364

BLAST of MS000470 vs. NCBI nr
Match: AZU90763.1 (WRKY transcription factor 13 [Siraitia grosvenorii] >QEE82235.1 WRKY transcription factor 13 [Siraitia grosvenorii])

HSP 1 Score: 516.2 bits (1328), Expect = 2.4e-142
Identity = 303/384 (78.91%), Postives = 324/384 (84.38%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSED---------GELLVQ 60
           MENIGEWEQKNLKNELLRGKELA+QLQ+YLNV+ SSSM AS S           GELLV+
Sbjct: 1   MENIGEWEQKNLKNELLRGKELARQLQIYLNVRQSSSMAASSSSSSSSSSSNGGGELLVK 60

Query: 61  KILSSYEKALSFF-SSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTW 120
           KILSSYEKALS   SSTGIQISESPS+ NGSPRSEDS     D DQR NASRKRN LPTW
Sbjct: 61  KILSSYEKALSLLSSSTGIQISESPSTLNGSPRSEDS-----DHDQR-NASRKRNILPTW 120

Query: 121 TQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRS 180
           TQKFQVSPGMA EGSLDDGFCWRKYGQK ILGAKHP RGYYRCTH+N+QGCLATKQVQR 
Sbjct: 121 TQKFQVSPGMALEGSLDDGFCWRKYGQKGILGAKHP-RGYYRCTHRNVQGCLATKQVQRC 180

Query: 181 DDDPTVFEITYRGNHTCSQVTNLSPPSAAP-EFQQPNSSNIDPNLVQKQSHSVPDQNQQS 240
           DDDPTVFEITYRGNHTCSQV+NL+PPSA P +FQQPNSS ID +LV  QSH  PDQNQQ+
Sbjct: 181 DDDPTVFEITYRGNHTCSQVSNLTPPSATPDQFQQPNSS-ID-SLV--QSHP-PDQNQQT 240

Query: 241 S-PDALLNSWASLRVITENLD-THEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEF 300
           S PDALLNSWASL+VITENLD THEPT FP FSYDPTS+Y+AA+HV+SAS TV VNFAEF
Sbjct: 241 SPPDALLNSWASLKVITENLDTTHEPT-FPPFSYDPTSNYEAANHVKSAS-TVDVNFAEF 300

Query: 301 PPSFLSP--TTSCSGLSYFSASSGGLSEGFVGNQKLQANKSELS--FPSPTSALNSQTLG 360
            PSF+SP  TTS SGL YF ASS  LSEGFVG+Q LQANK ELS  F SPTSA+NSQTLG
Sbjct: 301 SPSFISPTTTTSGSGLRYFPASSSVLSEGFVGHQNLQANKPELSEIFSSPTSAVNSQTLG 360

Query: 361 SEFPFGEVE-MEPSFTFDHTNFFS 367
            EFPFGEVE MEPSFTF +TNFFS
Sbjct: 361 LEFPFGEVELMEPSFTFHNTNFFS 370

BLAST of MS000470 vs. NCBI nr
Match: KAG6576671.1 (putative WRKY transcription factor 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 471.5 bits (1212), Expect = 6.7e-129
Identity = 271/383 (70.76%), Postives = 302/383 (78.85%), Query Frame = 0

Query: 1   MENIGEWEQ-KNLKNELLRGKELAKQLQLYLNVQPSSSMPA-------SPSEDG-ELLVQ 60
           M++ GEW+Q K LKNELL+GKELAKQLQ++LNV+PS+S  A       S S DG ELLVQ
Sbjct: 1   MDSFGEWDQKKKLKNELLKGKELAKQLQIHLNVRPSTSSMAAAAASSSSASNDGSELLVQ 60

Query: 61  KILSSYEKALSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWT 120
           KILSSYEKALS  SS GIQISESPSS NGSPRSEDSDREFKD D    +SR RN LPTWT
Sbjct: 61  KILSSYEKALSLLSSNGIQISESPSSLNGSPRSEDSDREFKDHDHTNASSRTRNILPTWT 120

Query: 121 QKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSD 180
           QKFQVSPGMA EGS DDGFCWRKYGQK ILGA+HP RGYYRCTH+NLQGCLATKQVQRSD
Sbjct: 121 QKFQVSPGMALEGSFDDGFCWRKYGQKGILGARHP-RGYYRCTHRNLQGCLATKQVQRSD 180

Query: 181 DDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSP 240
            DP VFEITYRG H+C+QV     PSA+ EFQQ N     P L+  Q      QNQQ+SP
Sbjct: 181 HDPNVFEITYRGTHSCTQVI----PSASTEFQQQN----HPLLLPDQR----VQNQQTSP 240

Query: 241 DALLNSWASLRVITENLD-THEPTLFPAFSYDPTSSYKAADHVESAS-MTVGVNFAEF-P 300
           DALL+SW SLRVITENLD TH+PTLF  F+YDPTS+Y+AAD VES S +   VNF+EF P
Sbjct: 241 DALLSSWPSLRVITENLDTTHDPTLFHPFNYDPTSNYEAADCVESGSTLRANVNFSEFSP 300

Query: 301 PSFLSPTTSCSGLSYFSASSGGLSEGFVGNQK---LQANKSELS--FPSPTSALNSQTLG 360
           P FLSPTTS SGLSYFSASS GLSEGF+G+ +   LQ NKSELS  F SPTSA NS T G
Sbjct: 301 PPFLSPTTSGSGLSYFSASSSGLSEGFIGHHQKLDLQPNKSELSEIFSSPTSASNSATPG 360

Query: 361 SEFPFGEVEMEPSFTFDHTNFFS 367
            +FPFGE++MEP+FTF +TNFFS
Sbjct: 361 LDFPFGELQMEPTFTFHNTNFFS 370

BLAST of MS000470 vs. ExPASy Swiss-Prot
Match: Q9SUP6 (Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 PE=1 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 5.7e-46
Identity = 128/305 (41.97%), Postives = 169/305 (55.41%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPS-----SSMPASPSEDGELLVQKILSSYEKA- 66
           WEQK L +EL+ G + AK+LQ  L   PS     SS   + +E  E+LV++I+SSYE++ 
Sbjct: 9   WEQKTLLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSL 68

Query: 67  --LSFFSSTGIQ----------------ISESPSSPNGSPRSED------SDREFKDQDQ 126
             L++ SS  +Q                + ESP+S NGSPRSE+      S      QD 
Sbjct: 69  LLLNWSSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHRQDY 128

Query: 127 RINASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHK 186
             N S+KR  LP W++K ++SP    EG  DD F WRKYGQKDILGAK P R YYRCTH+
Sbjct: 129 IFN-SKKRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFP-RSYYRCTHR 188

Query: 187 NLQGCLATKQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQ 246
           + Q C ATKQVQRSD D TVFE+TYRG HTCSQ    +PP A+PE +Q   + + P + Q
Sbjct: 189 STQNCWATKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPLASPEKRQ--DTRVKPAITQ 248

Query: 247 KQSHSVPDQNQQSSPDALLNSWASLRVITENLDTHE-----PTLFPAFSYDPTSSYKAAD 277
           K              D L +  ++L V T+ LD  +     P   P ++Y   +      
Sbjct: 249 KPK------------DILESLKSNLTVRTDGLDDGKDVFSFPDTPPFYNYGTING--EFG 295

BLAST of MS000470 vs. ExPASy Swiss-Prot
Match: Q8H0Y8 (Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 PE=1 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 9.7e-38
Identity = 93/201 (46.27%), Postives = 123/201 (61.19%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSS 66
           WE+++L NEL+ G + AKQLQ   +   S+S     +E  E L+  I+SS++KA+   + 
Sbjct: 6   WERRSLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILMLNG 65

Query: 67  TGIQ-----------------ISESPSSPNGSPRSEDSDREFKDQDQRIN-ASRKRNSLP 126
           +  Q                 +  SP+S  G+PRSE+    F  + +  N +S+KR  LP
Sbjct: 66  STTQHNPTIELAPDPLAHPGKVPGSPASITGNPRSEEF---FNVRSKEFNLSSKKRKMLP 125

Query: 127 TWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQ 186
            WT++ ++SP    EG  DD F WRKYGQKDILGAK P R YYRCT +N Q C ATKQVQ
Sbjct: 126 KWTEQVRISPERGLEGPHDDIFSWRKYGQKDILGAKFP-RSYYRCTFRNTQYCWATKQVQ 185

Query: 187 RSDDDPTVFEITYRGNHTCSQ 190
           RSD DPT+FE+TYRG HTCSQ
Sbjct: 186 RSDGDPTIFEVTYRGTHTCSQ 202

BLAST of MS000470 vs. ExPASy Swiss-Prot
Match: Q9FL62 (Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-34
Identity = 113/307 (36.81%), Postives = 164/307 (53.42%), Query Frame = 0

Query: 5   GEWEQ-KNLKNEL-LRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALS 64
           GEWE+ KN  NEL + G++ A Q              AS  E  E L +KIL SY K+L+
Sbjct: 8   GEWEKMKNEINELMIEGRDYAHQFG-----------SASSQETREHLAKKILQSYHKSLT 67

Query: 65  FFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAP 124
             + +G    +  S   GSP+S+DSD+E       I +S+K  S+P W+ K +++PG   
Sbjct: 68  IMNYSG--ELDQVSQGGGSPKSDDSDQE----PLVIKSSKK--SMPRWSSKVRIAPGAGV 127

Query: 125 EGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYR 184
           + +LDDGF WRKYGQKDILGAK P RGYYRCT++  QGC ATKQVQRSD++  + EI+YR
Sbjct: 128 DRTLDDGFSWRKYGQKDILGAKFP-RGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYR 187

Query: 185 GNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQ------------SS 244
           G H+CSQ  N+          +PN +    NL      SV + N Q            S+
Sbjct: 188 GIHSCSQAANVGTTMPIQNL-EPNQTQEHGNL-DMVKESVDNYNHQAHLHHNLHYPLSST 247

Query: 245 PDALLNSWASLRVITENLDTHEPTLF-----PAFSYDPTSSYKAADHVESASMTVGVN-- 291
           P+   N+   L++  +N++    T F      + +Y+  +S  A+    ++  TV +   
Sbjct: 248 PNLENNNAYMLQMRDQNIEYFGSTSFSSDLGTSINYNFPASGSASHSASNSPSTVPLESP 292

BLAST of MS000470 vs. ExPASy Swiss-Prot
Match: Q9SKD9 (Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.5e-25
Identity = 103/296 (34.80%), Postives = 156/296 (52.70%), Query Frame = 0

Query: 8   EQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSST 67
           E+K + NEL  GKELA +L    N++ +SS+     +  + L+  IL  Y+ A+   S  
Sbjct: 4   EEKLVINELELGKELANRLM--NNLKHTSSV-----DSNKTLISDILRIYQNAIFMLSFN 63

Query: 68  GIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAPE-GSL 127
                      N   RS + D     +D + N  +KR      T+K +V      E GS+
Sbjct: 64  --------QDKNILKRSLEID----GKDSK-NVFKKRKVSEKNTEKVKVFVATEQENGSI 123

Query: 128 DDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNHT 187
           DDG CWRKYGQK+I G+K+P R YYRCTH+  Q CLA KQVQ+SD DP++FE+ Y GNHT
Sbjct: 124 DDGHCWRKYGQKEIHGSKNP-RAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHT 183

Query: 188 CSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASLRVITE 247
           C+ +T+   P     F   + S  + N+ +     V +Q++   P        SL    E
Sbjct: 184 CNNITS---PKTTTNF---SVSLTNTNIFEGNRVHVTEQSEDMKPTKSEEVMISL----E 243

Query: 248 NLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGLS 303
           +L+ ++  +F  FS+   S+++  + V  +++ +G NF E     LSP TS S ++
Sbjct: 244 DLE-NKKNIFRTFSF---SNHEIENGVWKSNLFLG-NFVE----DLSPATSGSAIT 259

BLAST of MS000470 vs. ExPASy Swiss-Prot
Match: Q0DFT7 (Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 5.2e-23
Identity = 56/114 (49.12%), Postives = 74/114 (64.91%), Query Frame = 0

Query: 100 ASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQ 159
           A +KR  +    ++ +V+  +    SLDDG  WRKYGQKDILGAK+P R Y+RCTH++ Q
Sbjct: 80  AFKKRKGMAKVRRQVRVT-SVQDTASLDDGLSWRKYGQKDILGAKYP-RAYFRCTHRHTQ 139

Query: 160 GCLATKQVQRSDDDPTVFEITYRGNHTCSQ-----VTNLSPPSAAPEFQQPNSS 209
           GC ATKQVQR+D DP +F++ Y G+HTC Q         +PP  A + QQ  SS
Sbjct: 140 GCNATKQVQRADGDPLLFDVVYLGDHTCGQAAVAAAAQSAPPEHAGQEQQRQSS 191

BLAST of MS000470 vs. ExPASy TrEMBL
Match: A0A6J1CJ16 (probable WRKY transcription factor 53 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011439 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 1.5e-206
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 367
           HTNFFS
Sbjct: 361 HTNFFS 366

BLAST of MS000470 vs. ExPASy TrEMBL
Match: A0A6J1CH11 (probable WRKY transcription factor 53 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011439 PE=4 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.4e-204
Identity = 365/366 (99.73%), Postives = 365/366 (99.73%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHP RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHP-RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 367
           HTNFFS
Sbjct: 361 HTNFFS 365

BLAST of MS000470 vs. ExPASy TrEMBL
Match: A0A6J1CGF2 (probable WRKY transcription factor 53 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011439 PE=4 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 6.9e-204
Identity = 364/366 (99.45%), Postives = 364/366 (99.45%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK  SLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK--SLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 367
           HTNFFS
Sbjct: 361 HTNFFS 364

BLAST of MS000470 vs. ExPASy TrEMBL
Match: A0A3T0I8G0 (WRKY transcription factor 13 OS=Siraitia grosvenorii OX=190515 GN=WRKY13 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.2e-142
Identity = 303/384 (78.91%), Postives = 324/384 (84.38%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSED---------GELLVQ 60
           MENIGEWEQKNLKNELLRGKELA+QLQ+YLNV+ SSSM AS S           GELLV+
Sbjct: 1   MENIGEWEQKNLKNELLRGKELARQLQIYLNVRQSSSMAASSSSSSSSSSSNGGGELLVK 60

Query: 61  KILSSYEKALSFF-SSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTW 120
           KILSSYEKALS   SSTGIQISESPS+ NGSPRSEDS     D DQR NASRKRN LPTW
Sbjct: 61  KILSSYEKALSLLSSSTGIQISESPSTLNGSPRSEDS-----DHDQR-NASRKRNILPTW 120

Query: 121 TQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRS 180
           TQKFQVSPGMA EGSLDDGFCWRKYGQK ILGAKHP RGYYRCTH+N+QGCLATKQVQR 
Sbjct: 121 TQKFQVSPGMALEGSLDDGFCWRKYGQKGILGAKHP-RGYYRCTHRNVQGCLATKQVQRC 180

Query: 181 DDDPTVFEITYRGNHTCSQVTNLSPPSAAP-EFQQPNSSNIDPNLVQKQSHSVPDQNQQS 240
           DDDPTVFEITYRGNHTCSQV+NL+PPSA P +FQQPNSS ID +LV  QSH  PDQNQQ+
Sbjct: 181 DDDPTVFEITYRGNHTCSQVSNLTPPSATPDQFQQPNSS-ID-SLV--QSHP-PDQNQQT 240

Query: 241 S-PDALLNSWASLRVITENLD-THEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEF 300
           S PDALLNSWASL+VITENLD THEPT FP FSYDPTS+Y+AA+HV+SAS TV VNFAEF
Sbjct: 241 SPPDALLNSWASLKVITENLDTTHEPT-FPPFSYDPTSNYEAANHVKSAS-TVDVNFAEF 300

Query: 301 PPSFLSP--TTSCSGLSYFSASSGGLSEGFVGNQKLQANKSELS--FPSPTSALNSQTLG 360
            PSF+SP  TTS SGL YF ASS  LSEGFVG+Q LQANK ELS  F SPTSA+NSQTLG
Sbjct: 301 SPSFISPTTTTSGSGLRYFPASSSVLSEGFVGHQNLQANKPELSEIFSSPTSAVNSQTLG 360

Query: 361 SEFPFGEVE-MEPSFTFDHTNFFS 367
            EFPFGEVE MEPSFTF +TNFFS
Sbjct: 361 LEFPFGEVELMEPSFTFHNTNFFS 370

BLAST of MS000470 vs. ExPASy TrEMBL
Match: A0A6J1E4E2 (probable WRKY transcription factor 53 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430671 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 5.6e-129
Identity = 266/392 (67.86%), Postives = 300/392 (76.53%), Query Frame = 0

Query: 1   MENIGEWEQ-KNLKNELLRGKELAKQLQLYLNVQPSSSMPAS----------------PS 60
           M++ GEW+Q K LKNELL+GKELAKQLQ++LNV+PSSS  A+                 +
Sbjct: 1   MDSFGEWDQKKKLKNELLKGKELAKQLQIHLNVRPSSSSMAAAAAAAAAVSSSSSSSVSN 60

Query: 61  EDGELLVQKILSSYEKALSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK 120
           + GELLVQKILSSYEKALS  SS GIQISESPSS NGSPRSEDSDREFKD D    +SR 
Sbjct: 61  DGGELLVQKILSSYEKALSLLSSNGIQISESPSSLNGSPRSEDSDREFKDHDHTNASSRT 120

Query: 121 RNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLA 180
           RN LPTWTQKFQVSPGMA EGSLDDGFCWRKYGQK ILGA+HP RGYYRCTH+NLQGCLA
Sbjct: 121 RNILPTWTQKFQVSPGMALEGSLDDGFCWRKYGQKGILGARHP-RGYYRCTHRNLQGCLA 180

Query: 181 TKQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVP 240
           TKQVQRSD DP +FEITYRG H+C+QV     PSA+ EFQQ N   +    V        
Sbjct: 181 TKQVQRSDHDPNIFEITYRGTHSCTQVI----PSASTEFQQQNHPLLPDQRV-------- 240

Query: 241 DQNQQSSPDALLNSWASLRVITENLDT-HEPTLFPAFSYDPTSSYKAADHVESAS-MTVG 300
            QNQQ+SPDALL+SW SLRVITENLDT H+PTLF  F+YDPTS+Y+ AD VES S +   
Sbjct: 241 -QNQQTSPDALLSSWPSLRVITENLDTIHDPTLFHPFNYDPTSNYEVADRVESGSTLRAN 300

Query: 301 VNFAEF--PPSFLSPTTSCSGLSYFSASSGGLSEGFVGNQK---LQANKSELS--FPSPT 360
           VNF+EF  PP FLSPTTS SGLSYFSASS GLSEGF+G+ +   LQ NKSELS  F SPT
Sbjct: 301 VNFSEFSPPPPFLSPTTSGSGLSYFSASSSGLSEGFIGHHQKLDLQPNKSELSEIFSSPT 360

Query: 361 SALNSQTLGSEFPFGEVEMEPSFTFDHTNFFS 367
           SA NS T G +FPFGE++MEP+FTF +TNFFS
Sbjct: 361 SASNSATPGLDFPFGELQMEPTFTFHNTNFFS 378

BLAST of MS000470 vs. TAIR 10
Match: AT4G23810.1 (WRKY family transcription factor )

HSP 1 Score: 186.4 bits (472), Expect = 4.0e-47
Identity = 128/305 (41.97%), Postives = 169/305 (55.41%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPS-----SSMPASPSEDGELLVQKILSSYEKA- 66
           WEQK L +EL+ G + AK+LQ  L   PS     SS   + +E  E+LV++I+SSYE++ 
Sbjct: 9   WEQKTLLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSL 68

Query: 67  --LSFFSSTGIQ----------------ISESPSSPNGSPRSED------SDREFKDQDQ 126
             L++ SS  +Q                + ESP+S NGSPRSE+      S      QD 
Sbjct: 69  LLLNWSSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHRQDY 128

Query: 127 RINASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHK 186
             N S+KR  LP W++K ++SP    EG  DD F WRKYGQKDILGAK P R YYRCTH+
Sbjct: 129 IFN-SKKRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFP-RSYYRCTHR 188

Query: 187 NLQGCLATKQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQ 246
           + Q C ATKQVQRSD D TVFE+TYRG HTCSQ    +PP A+PE +Q   + + P + Q
Sbjct: 189 STQNCWATKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPLASPEKRQ--DTRVKPAITQ 248

Query: 247 KQSHSVPDQNQQSSPDALLNSWASLRVITENLDTHE-----PTLFPAFSYDPTSSYKAAD 277
           K              D L +  ++L V T+ LD  +     P   P ++Y   +      
Sbjct: 249 KPK------------DILESLKSNLTVRTDGLDDGKDVFSFPDTPPFYNYGTING--EFG 295

BLAST of MS000470 vs. TAIR 10
Match: AT4G11070.1 (WRKY family transcription factor )

HSP 1 Score: 159.1 bits (401), Expect = 6.9e-39
Identity = 93/201 (46.27%), Postives = 123/201 (61.19%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSS 66
           WE+++L NEL+ G + AKQLQ   +   S+S     +E  E L+  I+SS++KA+   + 
Sbjct: 6   WERRSLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILMLNG 65

Query: 67  TGIQ-----------------ISESPSSPNGSPRSEDSDREFKDQDQRIN-ASRKRNSLP 126
           +  Q                 +  SP+S  G+PRSE+    F  + +  N +S+KR  LP
Sbjct: 66  STTQHNPTIELAPDPLAHPGKVPGSPASITGNPRSEEF---FNVRSKEFNLSSKKRKMLP 125

Query: 127 TWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQ 186
            WT++ ++SP    EG  DD F WRKYGQKDILGAK P R YYRCT +N Q C ATKQVQ
Sbjct: 126 KWTEQVRISPERGLEGPHDDIFSWRKYGQKDILGAKFP-RSYYRCTFRNTQYCWATKQVQ 185

Query: 187 RSDDDPTVFEITYRGNHTCSQ 190
           RSD DPT+FE+TYRG HTCSQ
Sbjct: 186 RSDGDPTIFEVTYRGTHTCSQ 202

BLAST of MS000470 vs. TAIR 10
Match: AT5G24110.1 (WRKY DNA-binding protein 30 )

HSP 1 Score: 148.7 bits (374), Expect = 9.3e-36
Identity = 113/307 (36.81%), Postives = 164/307 (53.42%), Query Frame = 0

Query: 5   GEWEQ-KNLKNEL-LRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALS 64
           GEWE+ KN  NEL + G++ A Q              AS  E  E L +KIL SY K+L+
Sbjct: 8   GEWEKMKNEINELMIEGRDYAHQFG-----------SASSQETREHLAKKILQSYHKSLT 67

Query: 65  FFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAP 124
             + +G    +  S   GSP+S+DSD+E       I +S+K  S+P W+ K +++PG   
Sbjct: 68  IMNYSG--ELDQVSQGGGSPKSDDSDQE----PLVIKSSKK--SMPRWSSKVRIAPGAGV 127

Query: 125 EGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYR 184
           + +LDDGF WRKYGQKDILGAK P RGYYRCT++  QGC ATKQVQRSD++  + EI+YR
Sbjct: 128 DRTLDDGFSWRKYGQKDILGAKFP-RGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYR 187

Query: 185 GNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQ------------SS 244
           G H+CSQ  N+          +PN +    NL      SV + N Q            S+
Sbjct: 188 GIHSCSQAANVGTTMPIQNL-EPNQTQEHGNL-DMVKESVDNYNHQAHLHHNLHYPLSST 247

Query: 245 PDALLNSWASLRVITENLDTHEPTLF-----PAFSYDPTSSYKAADHVESASMTVGVN-- 291
           P+   N+   L++  +N++    T F      + +Y+  +S  A+    ++  TV +   
Sbjct: 248 PNLENNNAYMLQMRDQNIEYFGSTSFSSDLGTSINYNFPASGSASHSASNSPSTVPLESP 292

BLAST of MS000470 vs. TAIR 10
Match: AT4G11070.2 (WRKY family transcription factor )

HSP 1 Score: 147.1 bits (370), Expect = 2.7e-35
Identity = 87/184 (47.28%), Postives = 113/184 (61.41%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSS 66
           WE+++L NEL+ G + AKQLQ  L +  S++                  + E A    + 
Sbjct: 6   WERRSLLNELIHGLKAAKQLQAILMLNGSTTQHN--------------PTIELAPDPLAH 65

Query: 67  TGIQISESPSSPNGSPRSEDSDREFKDQDQRIN-ASRKRNSLPTWTQKFQVSPGMAPEGS 126
            G ++  SP+S  G+PRSE+    F  + +  N +S+KR  LP WT++ ++SP    EG 
Sbjct: 66  PG-KVPGSPASITGNPRSEEF---FNVRSKEFNLSSKKRKMLPKWTEQVRISPERGLEGP 125

Query: 127 LDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNH 186
            DD F WRKYGQKDILGAK P R YYRCT +N Q C ATKQVQRSD DPT+FE+TYRG H
Sbjct: 126 HDDIFSWRKYGQKDILGAKFP-RSYYRCTFRNTQYCWATKQVQRSDGDPTIFEVTYRGTH 170

Query: 187 TCSQ 190
           TCSQ
Sbjct: 186 TCSQ 170

BLAST of MS000470 vs. TAIR 10
Match: AT2G46400.1 (WRKY DNA-binding protein 46 )

HSP 1 Score: 118.6 bits (296), Expect = 1.0e-26
Identity = 103/296 (34.80%), Postives = 156/296 (52.70%), Query Frame = 0

Query: 8   EQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSST 67
           E+K + NEL  GKELA +L    N++ +SS+     +  + L+  IL  Y+ A+   S  
Sbjct: 4   EEKLVINELELGKELANRLM--NNLKHTSSV-----DSNKTLISDILRIYQNAIFMLSFN 63

Query: 68  GIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAPE-GSL 127
                      N   RS + D     +D + N  +KR      T+K +V      E GS+
Sbjct: 64  --------QDKNILKRSLEID----GKDSK-NVFKKRKVSEKNTEKVKVFVATEQENGSI 123

Query: 128 DDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNHT 187
           DDG CWRKYGQK+I G+K+P R YYRCTH+  Q CLA KQVQ+SD DP++FE+ Y GNHT
Sbjct: 124 DDGHCWRKYGQKEIHGSKNP-RAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHT 183

Query: 188 CSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASLRVITE 247
           C+ +T+   P     F   + S  + N+ +     V +Q++   P        SL    E
Sbjct: 184 CNNITS---PKTTTNF---SVSLTNTNIFEGNRVHVTEQSEDMKPTKSEEVMISL----E 243

Query: 248 NLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGLS 303
           +L+ ++  +F  FS+   S+++  + V  +++ +G NF E     LSP TS S ++
Sbjct: 244 DLE-NKKNIFRTFSF---SNHEIENGVWKSNLFLG-NFVE----DLSPATSGSAIT 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140883.13.1e-206100.00probable WRKY transcription factor 53 isoform X1 [Momordica charantia][more]
XP_022140884.12.9e-20499.73probable WRKY transcription factor 53 isoform X2 [Momordica charantia][more]
XP_022140885.11.4e-20399.45probable WRKY transcription factor 53 isoform X3 [Momordica charantia][more]
AZU90763.12.4e-14278.91WRKY transcription factor 13 [Siraitia grosvenorii] >QEE82235.1 WRKY transcripti... [more]
KAG6576671.16.7e-12970.76putative WRKY transcription factor 53, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
Q9SUP65.7e-4641.97Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 ... [more]
Q8H0Y89.7e-3846.27Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 ... [more]
Q9FL621.3e-3436.81Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 ... [more]
Q9SKD91.5e-2534.80Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 ... [more]
Q0DFT75.2e-2349.12Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 P... [more]
Match NameE-valueIdentityDescription
A0A6J1CJ161.5e-206100.00probable WRKY transcription factor 53 isoform X1 OS=Momordica charantia OX=3673 ... [more]
A0A6J1CH111.4e-20499.73probable WRKY transcription factor 53 isoform X2 OS=Momordica charantia OX=3673 ... [more]
A0A6J1CGF26.9e-20499.45probable WRKY transcription factor 53 isoform X3 OS=Momordica charantia OX=3673 ... [more]
A0A3T0I8G01.2e-14278.91WRKY transcription factor 13 OS=Siraitia grosvenorii OX=190515 GN=WRKY13 PE=2 SV... [more]
A0A6J1E4E25.6e-12967.86probable WRKY transcription factor 53 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT4G23810.14.0e-4741.97WRKY family transcription factor [more]
AT4G11070.16.9e-3946.27WRKY family transcription factor [more]
AT5G24110.19.3e-3636.81WRKY DNA-binding protein 30 [more]
AT4G11070.22.7e-3547.28WRKY family transcription factor [more]
AT2G46400.11.0e-2634.80WRKY DNA-binding protein 46 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 126..189
e-value: 4.1E-39
score: 145.9
IPR003657WRKY domainPFAMPF03106WRKYcoord: 127..187
e-value: 1.3E-24
score: 86.2
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 121..185
score: 20.162411
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 112..182
e-value: 1.5E-25
score: 90.6
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 124..189
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..109
NoneNo IPR availablePANTHERPTHR32096:SF36WRKY TRANSCRIPTION FACTOR 30-RELATEDcoord: 1..363
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 1..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000470.1MS000470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding