MS000469 (gene) Bitter gourd (TR) v1

Overview
NameMS000469
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptiontranscription factor SPEECHLESS
Locationscaffold44: 1917436 .. 1918752 (+)
RNA-Seq ExpressionMS000469
SyntenyMS000469
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGATAGTTTGGCTGATTTTTTCCAACAAAATGAAGAATTTGTCGACAATCCCCATTTGGAAGGAGACGACCTCTTCAGCATCTTTGAGAGTTTGGAGAGTGTTGCAGAGTTCACCGTCATCGAGGACGACATCGAAGCTTTCAAAGACGGTGAAGAGACCACGAGCTTGGTCTTCTCCGAGACTGAGCTTGAGACTTCGCCTAAAAGCAAGCGCTTGAAGATGACGACCACGATGTCGTCCCCAATGGCAGCTCCCTCCGAAGAACCAAACCCAGATGGGCCTCAACGAATGTCTCACATTACGGTGGAGCGCAATCGTAGGAAGCAAATGAATGAGCACTTGTCCGTATTAAGGTCACTCATGCCGTGCTTCTATGTCAAGAAAGTAAGCACTTTTGAGTTTAGTGGTGATTTCTTACCGTATATCAGACTCAGAATAAAGTTCCTTCCATGTTCGAACTTTTATAATCTCATTTCCTCCACTTACCATATGCATTATAAGCATGCTTTTAAGGGTTAAGTGGTTAACATTTGTTGATGAGGGTTTTATGTAAGATTTTCACATATATGCTTTGATTTTTACTTCACTCACGAATCCAAGTAATTAATTAATGTTGTGTTCTTCTTCTTCTTCTTCTTCTTGAATGCACTTGACATAATTCAGGGAGATCAAGCTTCAATAATTGGAGGGGTGGTGGAATACATCAAAGAGTTGCAGCAAGTTCTTCAATCTCTAGAGGCCAAAAAGCAAAGGAAAGTTTATAGTGAAGTGTTGAGCCCTAGGGTAGTTTCAAGTCCAAGAGCTAATTTGGCAATGAGTCCAAGAAAACCACCACTGAGTCCACGCCTTAACTTGCCAATTAGTCCAAGAACTCCACAACCCACTAGCCCTTACAGCAAGCCTCCCCGGTTGCAGCAACCTCCCAGCACCGGCACCGCCGCCGCCGCCGCCGCTGGTGGTGGTGGTTATAATATGGTCTCTTCTGCAGGCAGCTGCACCACCAACGTCAACAACAATAACTCATTAGAGTTGCCGTCGCCTTGTAACTCTTCCTCCACTACCACAAATTCCTCCATTGACATGAATGCCAATGATCTTGTGGCCAATTCTAAATCCGCCATAGCTGAAGTTGAGGTGAAGTTTACGGGTCCAAATGTGGTTTTGAAGACGGTTTCTCCTCCAATCCCTGGTCAGGCTGTGAAGATCATTTCTGCACTTGAACAACTCTCTCTTGAAATTCTCCATGTCAAGATCACCACCCTTGATGAAACCATGCTCAACTCTTTTACAATTAAGGTATATACACAACAA

mRNA sequence

ATGGTTGATAGTTTGGCTGATTTTTTCCAACAAAATGAAGAATTTGTCGACAATCCCCATTTGGAAGGAGACGACCTCTTCAGCATCTTTGAGAGTTTGGAGAGTGTTGCAGAGTTCACCGTCATCGAGGACGACATCGAAGCTTTCAAAGACGGTGAAGAGACCACGAGCTTGGTCTTCTCCGAGACTGAGCTTGAGACTTCGCCTAAAAGCAAGCGCTTGAAGATGACGACCACGATGTCGTCCCCAATGGCAGCTCCCTCCGAAGAACCAAACCCAGATGGGCCTCAACGAATGTCTCACATTACGGTGGAGCGCAATCGTAGGAAGCAAATGAATGAGCACTTGTCCGTATTAAGGTCACTCATGCCGTGCTTCTATGTCAAGAAAGGAGATCAAGCTTCAATAATTGGAGGGGTGGTGGAATACATCAAAGAGTTGCAGCAAGTTCTTCAATCTCTAGAGGCCAAAAAGCAAAGGAAAGTTTATAGTGAAGTGTTGAGCCCTAGGGTAGTTTCAAGTCCAAGAGCTAATTTGGCAATGAGTCCAAGAAAACCACCACTGAGTCCACGCCTTAACTTGCCAATTAGTCCAAGAACTCCACAACCCACTAGCCCTTACAGCAAGCCTCCCCGGTTGCAGCAACCTCCCAGCACCGGCACCGCCGCCGCCGCCGCCGCTGGTGGTGGTGGTTATAATATGGTCTCTTCTGCAGGCAGCTGCACCACCAACGTCAACAACAATAACTCATTAGAGTTGCCGTCGCCTTGTAACTCTTCCTCCACTACCACAAATTCCTCCATTGACATGAATGCCAATGATCTTGTGGCCAATTCTAAATCCGCCATAGCTGAAGTTGAGGTGAAGTTTACGGGTCCAAATGTGGTTTTGAAGACGGTTTCTCCTCCAATCCCTGGTCAGGCTGTGAAGATCATTTCTGCACTTGAACAACTCTCTCTTGAAATTCTCCATGTCAAGATCACCACCCTTGATGAAACCATGCTCAACTCTTTTACAATTAAGGTATATACACAACAA

Coding sequence (CDS)

ATGGTTGATAGTTTGGCTGATTTTTTCCAACAAAATGAAGAATTTGTCGACAATCCCCATTTGGAAGGAGACGACCTCTTCAGCATCTTTGAGAGTTTGGAGAGTGTTGCAGAGTTCACCGTCATCGAGGACGACATCGAAGCTTTCAAAGACGGTGAAGAGACCACGAGCTTGGTCTTCTCCGAGACTGAGCTTGAGACTTCGCCTAAAAGCAAGCGCTTGAAGATGACGACCACGATGTCGTCCCCAATGGCAGCTCCCTCCGAAGAACCAAACCCAGATGGGCCTCAACGAATGTCTCACATTACGGTGGAGCGCAATCGTAGGAAGCAAATGAATGAGCACTTGTCCGTATTAAGGTCACTCATGCCGTGCTTCTATGTCAAGAAAGGAGATCAAGCTTCAATAATTGGAGGGGTGGTGGAATACATCAAAGAGTTGCAGCAAGTTCTTCAATCTCTAGAGGCCAAAAAGCAAAGGAAAGTTTATAGTGAAGTGTTGAGCCCTAGGGTAGTTTCAAGTCCAAGAGCTAATTTGGCAATGAGTCCAAGAAAACCACCACTGAGTCCACGCCTTAACTTGCCAATTAGTCCAAGAACTCCACAACCCACTAGCCCTTACAGCAAGCCTCCCCGGTTGCAGCAACCTCCCAGCACCGGCACCGCCGCCGCCGCCGCCGCTGGTGGTGGTGGTTATAATATGGTCTCTTCTGCAGGCAGCTGCACCACCAACGTCAACAACAATAACTCATTAGAGTTGCCGTCGCCTTGTAACTCTTCCTCCACTACCACAAATTCCTCCATTGACATGAATGCCAATGATCTTGTGGCCAATTCTAAATCCGCCATAGCTGAAGTTGAGGTGAAGTTTACGGGTCCAAATGTGGTTTTGAAGACGGTTTCTCCTCCAATCCCTGGTCAGGCTGTGAAGATCATTTCTGCACTTGAACAACTCTCTCTTGAAATTCTCCATGTCAAGATCACCACCCTTGATGAAACCATGCTCAACTCTTTTACAATTAAGGTATATACACAACAA

Protein sequence

MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVFSETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKVYTQQ
Homology
BLAST of MS000469 vs. NCBI nr
Match: XP_022141167.1 (transcription factor SPEECHLESS [Momordica charantia])

HSP 1 Score: 625.2 bits (1611), Expect = 3.4e-175
Identity = 341/343 (99.42%), Postives = 342/343 (99.71%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF 60
           MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF
Sbjct: 1   MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF 60

Query: 61  SETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSVLR 120
           SETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSVLR
Sbjct: 61  SETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSVLR 120

Query: 121 SLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRANLA 180
           SLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRANLA
Sbjct: 121 SLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRANLA 180

Query: 181 MSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGT-AAAAAAGGGGYNMVSSAG 240
           MSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGT AAAAAAGGGGYNMVSSAG
Sbjct: 181 MSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGTAAAAAAAGGGGYNMVSSAG 240

Query: 241 SCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEVKFTGPNVVLKT 300
           SCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEVKFTGPNVVLKT
Sbjct: 241 SCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEVKFTGPNVVLKT 300

Query: 301 VSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKV 343
           VSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIK+
Sbjct: 301 VSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKI 343

BLAST of MS000469 vs. NCBI nr
Match: XP_038906043.1 (transcription factor SPEECHLESS [Benincasa hispida])

HSP 1 Score: 506.1 bits (1302), Expect = 2.3e-139
Identity = 302/366 (82.51%), Postives = 313/366 (85.52%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVD----NPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETT 60
           MVD+ AD FQQNEEFVD    NPH EGDDLFSIFESLESVAEFT+IEDDIE  KDGEETT
Sbjct: 1   MVDTFADLFQQNEEFVDHTTSNPHFEGDDLFSIFESLESVAEFTMIEDDIEPTKDGEETT 60

Query: 61  SLVFSETELETSPKSKRLKMTTTMSSPMA-----APSEEPNPDGPQRMSHITVERNRRKQ 120
           SLVFSETELETSPKSKRLK TT  S  +A     + SE+PN DGPQRMSHITVERNRRKQ
Sbjct: 61  SLVFSETELETSPKSKRLKTTTATSPTIAMTTGLSSSEDPNLDGPQRMSHITVERNRRKQ 120

Query: 121 MNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRV 180
           MNEHL+VLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRV
Sbjct: 121 MNEHLTVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRV 180

Query: 181 VSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK-PPRLQQPPSTGTAAAAAAGGG 240
           VSSPR NL MSPRKPPLSPRLNLPISPRTPQPTSPYSK PPRLQQPPS    AAA AG G
Sbjct: 181 VSSPRPNLPMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPPRLQQPPS--AVAAAVAGDG 240

Query: 241 GYNMVSSAGSCTTNVNN--NNSLELPSPCNSSSTTTNSSIDM------------NANDLV 300
           GYN +SS+ SCT+N+ N  NNSLE PSPCNS S TTNSSID             N NDLV
Sbjct: 241 GYN-ISSSSSCTSNIANKSNNSLE-PSPCNSCS-TTNSSIDNISSNNNNNNNNGNNNDLV 300

Query: 301 ANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLN 343
           ANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETM N
Sbjct: 301 ANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMFN 360

BLAST of MS000469 vs. NCBI nr
Match: XP_004149468.1 (transcription factor SPEECHLESS [Cucumis sativus] >KGN57858.1 hypothetical protein Csa_011454 [Cucumis sativus])

HSP 1 Score: 498.0 bits (1281), Expect = 6.4e-137
Identity = 297/365 (81.37%), Postives = 310/365 (84.93%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVD---NPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTS 60
           MVD+ AD FQQN+EFVD   NPH EGDDLFSIFESLE+VAEFT+IEDDIE  KDGEETTS
Sbjct: 1   MVDTFADLFQQNDEFVDHTLNPHFEGDDLFSIFESLENVAEFTMIEDDIEPVKDGEETTS 60

Query: 61  LVFSETELETSPKSKRLKM------TTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQ 120
           LVFSETELETSPK+KRLK       T  M+S   + SE+PNPDGPQRMSHITVERNRRKQ
Sbjct: 61  LVFSETELETSPKTKRLKTIAATSPTAAMTSTGLSSSEDPNPDGPQRMSHITVERNRRKQ 120

Query: 121 MNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK-VYSEVLSPR 180
           MNEHL+VLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK VYSEVLSPR
Sbjct: 121 MNEHLTVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVVYSEVLSPR 180

Query: 181 VVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK-PPRLQQPPSTGTAAAAAAGG 240
           VVSSPR NLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK PPRLQQPPS      AA GG
Sbjct: 181 VVSSPRPNLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPPRLQQPPS------AATGG 240

Query: 241 GGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSID------------MNANDLVA 300
           G YN+ SS+ S TTN+ NNNSLE PSPCNS S TTNSSID             N NDLVA
Sbjct: 241 GAYNIPSSSSS-TTNMANNNSLE-PSPCNSCS-TTNSSIDNNNNNNNSNSNSNNNNDLVA 300

Query: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNS 343
           NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQA KIISALEQLSLEILHVKITTLDETM NS
Sbjct: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAFKIISALEQLSLEILHVKITTLDETMFNS 356

BLAST of MS000469 vs. NCBI nr
Match: XP_008465382.1 (PREDICTED: transcription factor SPEECHLESS [Cucumis melo] >KAA0050458.1 transcription factor SPEECHLESS [Cucumis melo var. makuwa] >TYK03138.1 transcription factor SPEECHLESS [Cucumis melo var. makuwa])

HSP 1 Score: 496.5 bits (1277), Expect = 1.8e-136
Identity = 296/365 (81.10%), Postives = 309/365 (84.66%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVD---NPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTS 60
           MVD+ AD FQQN+EFVD   NPH EGDDLFSIFESLE+VAEFT+IEDD E  KDGEETTS
Sbjct: 1   MVDTFADLFQQNDEFVDHTLNPHFEGDDLFSIFESLENVAEFTMIEDDTEPVKDGEETTS 60

Query: 61  LVFSETELETSPKSKRLKM------TTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQ 120
           LVFSETELETSPK+KRLK       T  M+S   + SE+PNPDGPQRMSHITVERNRRKQ
Sbjct: 61  LVFSETELETSPKTKRLKTIAATSPTAAMTSTGLSSSEDPNPDGPQRMSHITVERNRRKQ 120

Query: 121 MNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK-VYSEVLSPR 180
           MNEHL+VLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK VYSEVLSPR
Sbjct: 121 MNEHLTVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVVYSEVLSPR 180

Query: 181 VVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK-PPRLQQPPSTGTAAAAAAGG 240
           VVSSPR NLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK PPRLQQ PS      AA GG
Sbjct: 181 VVSSPRPNLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPPRLQQTPS------AATGG 240

Query: 241 GGYNMVSSAGSCTTNV-NNNNSLELPSPCNSSSTTTNSSID-----------MNANDLVA 300
           G YN +SS+ S TTN+ NNNNSLE PSPCNS S TTNSSID            N NDLVA
Sbjct: 241 GAYNNISSSSSSTTNMANNNNSLE-PSPCNSCS-TTNSSIDNNNNNNSNSNSNNNNDLVA 300

Query: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNS 343
           NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQA KIISALEQLSLEILHVKITTLDETM NS
Sbjct: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAFKIISALEQLSLEILHVKITTLDETMFNS 357

BLAST of MS000469 vs. NCBI nr
Match: KAG6574226.1 (Transcription factor SPEECHLESS, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013294.1 Transcription factor SPEECHLESS, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 490.7 bits (1262), Expect = 1.0e-134
Identity = 285/352 (80.97%), Postives = 303/352 (86.08%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVDN--PHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSL 60
           MV SLA FFQ N+EFVDN  PHL+GDDLFSIFESLESVAEFT+IEDD E  KDGEETTSL
Sbjct: 1   MVASLAQFFQPNQEFVDNTTPHLQGDDLFSIFESLESVAEFTMIEDDTEPTKDGEETTSL 60

Query: 61  VFSETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSV 120
           VFSETELE SPK+KRLK TTT     A+P EE NPDG QR+SHITVERNRRKQMNEHLS+
Sbjct: 61  VFSETELEASPKTKRLKTTTTAK---ASPLEEANPDGTQRISHITVERNRRKQMNEHLSI 120

Query: 121 LRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRAN 180
           LR+LMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAK+QRKVYSE LSPRVVSSPR N
Sbjct: 121 LRTLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKRQRKVYSEGLSPRVVSSPRPN 180

Query: 181 LAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGTAAAAAAGGGGYNMVSSA 240
           L MSPRKPPLSPRLNLPISPRTPQPTSPY+KPPRLQQPP+T  AA A++G          
Sbjct: 181 LQMSPRKPPLSPRLNLPISPRTPQPTSPYTKPPRLQQPPTTVAAATASSG---------- 240

Query: 241 GSCTTNV-NNNNSLELPSPCNSSSTTTNSSIDMN-----ANDLVANSKSAIAEVEVKFTG 300
             CTTN+ NNNNSLE PSPCNSSSTT +S  + N      NDLVANSKSAIAEVEVKFTG
Sbjct: 241 --CTTNMANNNNSLE-PSPCNSSSTTYSSIDNANNANNANNDLVANSKSAIAEVEVKFTG 300

Query: 301 PNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKVYT 345
           PNVVLKTVSPPIPGQAVKIISALE LSLEILHVKITTLD+TM+NSFTIKVYT
Sbjct: 301 PNVVLKTVSPPIPGQAVKIISALEHLSLEILHVKITTLDQTMINSFTIKVYT 336

BLAST of MS000469 vs. ExPASy Swiss-Prot
Match: Q700C7 (Transcription factor SPEECHLESS OS=Arabidopsis thaliana OX=3702 GN=SPCH PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.2e-75
Identity = 196/369 (53.12%), Postives = 240/369 (65.04%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF 60
           M + + DF ++  EFVD   L GDDLF+I ESLE   E +         KDG  ++  + 
Sbjct: 1   MQEIIPDFLEEC-EFVDT-SLAGDDLFAILESLEGAGEISPTAASTP--KDGTTSSKELV 60

Query: 61  SETELE-TSPKSKRLKMTTTMSSPM------AAPSEEPNPDGPQRMSHITVERNRRKQMN 120
            + + E +SPK K+ ++ T                E+   DG Q+MSH+TVERNRRKQMN
Sbjct: 61  KDQDYENSSPKRKKQRLETRKEEDEEEEDGDGEAEEDNKQDGQQKMSHVTVERNRRKQMN 120

Query: 121 EHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVS 180
           EHL+VLRSLMPCFYVK+GDQASIIGGVVEYI ELQQVLQSLEAKKQRK Y+EVLSPRVV 
Sbjct: 121 EHLTVLRSLMPCFYVKRGDQASIIGGVVEYISELQQVLQSLEAKKQRKTYAEVLSPRVVP 180

Query: 181 SPRAN-LAMSPRKPPLSPRLN----------LPISPRTPQPTSPY-SKPPRL---QQPPS 240
           SPR +   +SPRKPPLSPR+N           PISPRTPQPTSPY + PP+L    QPP 
Sbjct: 181 SPRPSPPVLSPRKPPLSPRINHHQIHHHLLLPPISPRTPQPTSPYRAIPPQLPLIPQPP- 240

Query: 241 TGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMN-----AN 300
                            SS  SC       +SL  P P + +S++++ S+  N      N
Sbjct: 241 -------------LRSYSSLASC-------SSLGDPPPYSPASSSSSPSVSSNHESSVIN 300

Query: 301 DLVANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDET 343
           +LVANSKSA+A+VEVKF+G NV+LKTVS  IPGQ +KII+ALE L+LEIL V I T+DET
Sbjct: 301 ELVANSKSALADVEVKFSGANVLLKTVSHKIPGQVMKIIAALEDLALEILQVNINTVDET 344

BLAST of MS000469 vs. ExPASy Swiss-Prot
Match: Q9M8K6 (Transcription factor MUTE OS=Arabidopsis thaliana OX=3702 GN=MUTE PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-27
Identity = 98/244 (40.16%), Postives = 126/244 (51.64%), Query Frame = 0

Query: 99  MSHITVERNRRKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKK 158
           MSHI VERNRR+QMNEHL  LRSL PCFY+K+GDQASIIGGV+E+IKELQQ++Q LE+KK
Sbjct: 1   MSHIAVERNRRRQMNEHLKSLRSLTPCFYIKRGDQASIIGGVIEFIKELQQLVQVLESKK 60

Query: 159 QRKVYSEVLSPRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPS 218
           +RK                              LN P  P   Q   P            
Sbjct: 61  RRKT-----------------------------LNRPSFPYDHQTIEP------------ 120

Query: 219 TGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVAN 278
                            SS G+ TT V  +  +E     N  +T+T   +    N     
Sbjct: 121 -----------------SSLGAATTRVPFSR-IE-----NVMTTSTFKEVGACCN----- 173

Query: 279 SKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSF 338
             S  A VE K +G NVVL+ VS  I GQ VKIIS LE+LS ++LH+ I++++ET+L  F
Sbjct: 181 --SPHANVEAKISGSNVVLRVVSRRIVGQLVKIISVLEKLSFQVLHLNISSMEETVLYFF 173

Query: 339 TIKV 343
            +K+
Sbjct: 241 VVKI 173

BLAST of MS000469 vs. ExPASy Swiss-Prot
Match: Q56YJ8 (Transcription factor FAMA OS=Arabidopsis thaliana OX=3702 GN=FAMA PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.1e-26
Identity = 101/297 (34.01%), Postives = 141/297 (47.47%), Query Frame = 0

Query: 49  FKDGEETTSLVFSETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNR 108
           F  GEE      + T+ E   K KR + + T        SEE      QRM+HI VERNR
Sbjct: 158 FIGGEEEDRENKNVTKKEVKSKRKRARTSKT--------SEEVE---SQRMTHIAVERNR 217

Query: 109 RKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLS 168
           RKQMNEHL VLRSLMP  YV++GDQASIIGG +E+++EL+Q+LQ LE++K+R++  E   
Sbjct: 218 RKQMNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRILGET-- 277

Query: 169 PRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGTAAAAAAG 228
                                      ++  T   +SP +      QP           G
Sbjct: 278 ------------------------GRDMTTTTTSSSSPITTVANQAQPLIITGNVTELEG 337

Query: 229 GGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEV 288
           GGG                                          +  A +KS +A+VEV
Sbjct: 338 GGG----------------------------------------LREETAENKSCLADVEV 377

Query: 289 KFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKVYTQ 346
           K  G + ++K +S   PGQ +K I+ALE L L ILH  ITT+++T+L SF +K+ ++
Sbjct: 398 KLLGFDAMIKILSRRRPGQLIKTIAALEDLHLSILHTNITTMEQTVLYSFNVKITSE 377

BLAST of MS000469 vs. ExPASy Swiss-Prot
Match: Q9C7T4 (Transcription factor bHLH96 OS=Arabidopsis thaliana OX=3702 GN=BHLH96 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.6e-16
Identity = 78/246 (31.71%), Postives = 113/246 (45.93%), Query Frame = 0

Query: 97  QRMSHITVERNRRKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEA 156
           QRM+HI VERNRRKQMNE+L+VLRSLMP +Y ++GDQASI+GG + Y+KEL+  LQS+E 
Sbjct: 123 QRMTHIAVERNRRKQMNEYLAVLRSLMPPYYAQRGDQASIVGGAINYLKELEHHLQSME- 182

Query: 157 KKQRKVYSEVLSPRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQP 216
                       P V ++     A   +    S   + P S     P   YS  P     
Sbjct: 183 ------------PPVKTATEDTGAGHDQTKTTSASSSGPFSDFFAFP--QYSNRP----- 242

Query: 217 PSTGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLV 276
               T+AAAA G                                                
Sbjct: 243 ----TSAAAAEG------------------------------------------------ 290

Query: 277 ANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLN 336
                 +AE+EV     +  LK ++   P Q +K++S+++ L L +LH+ +TT D+++L 
Sbjct: 303 ------MAEIEVTMVESHASLKILAKKRPRQLLKLVSSIQSLRLTLLHLNVTTRDDSVLY 290

Query: 337 SFTIKV 343
           S ++KV
Sbjct: 363 SISVKV 290

BLAST of MS000469 vs. ExPASy Swiss-Prot
Match: Q9SK91 (Transcription factor bHLH94 OS=Arabidopsis thaliana OX=3702 GN=BHLH94 PE=1 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 3.4e-16
Identity = 73/246 (29.67%), Postives = 109/246 (44.31%), Query Frame = 0

Query: 97  QRMSHITVERNRRKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEA 156
           QRM+HI VERNRRKQMNE+L+VLRSLMP  Y ++GDQASI+GG + Y+KEL+ +LQS+E 
Sbjct: 113 QRMTHIAVERNRRKQMNEYLAVLRSLMPSSYAQRGDQASIVGGAINYVKELEHILQSMEP 172

Query: 157 KKQRKVYSEVLSPRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQP 216
           K+                                                     R   P
Sbjct: 173 KR----------------------------------------------------TRTHDP 232

Query: 217 PSTGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLV 276
               T+ ++  G                             +    +T SS D      V
Sbjct: 233 KGDKTSTSSLVG-----------------------PFTDFFSFPQYSTKSSSD------V 277

Query: 277 ANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLN 336
             S S+ AE+EV     +  +K ++   P Q +K+I++L+ L L +LH+ +TTL  ++L 
Sbjct: 293 PESSSSPAEIEVTVAESHANIKIMTKKKPRQLLKLITSLQSLRLTLLHLNVTTLHNSILY 277

Query: 337 SFTIKV 343
           S +++V
Sbjct: 353 SISVRV 277

BLAST of MS000469 vs. ExPASy TrEMBL
Match: A0A6J1CJ40 (transcription factor SPEECHLESS OS=Momordica charantia OX=3673 GN=LOC111011625 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.7e-175
Identity = 341/343 (99.42%), Postives = 342/343 (99.71%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF 60
           MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF
Sbjct: 1   MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF 60

Query: 61  SETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSVLR 120
           SETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSVLR
Sbjct: 61  SETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSVLR 120

Query: 121 SLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRANLA 180
           SLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRANLA
Sbjct: 121 SLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRANLA 180

Query: 181 MSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGT-AAAAAAGGGGYNMVSSAG 240
           MSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGT AAAAAAGGGGYNMVSSAG
Sbjct: 181 MSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGTAAAAAAAGGGGYNMVSSAG 240

Query: 241 SCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEVKFTGPNVVLKT 300
           SCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEVKFTGPNVVLKT
Sbjct: 241 SCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEVKFTGPNVVLKT 300

Query: 301 VSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKV 343
           VSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIK+
Sbjct: 301 VSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKI 343

BLAST of MS000469 vs. ExPASy TrEMBL
Match: A0A0A0L7S9 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354520 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 3.1e-137
Identity = 297/365 (81.37%), Postives = 310/365 (84.93%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVD---NPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTS 60
           MVD+ AD FQQN+EFVD   NPH EGDDLFSIFESLE+VAEFT+IEDDIE  KDGEETTS
Sbjct: 1   MVDTFADLFQQNDEFVDHTLNPHFEGDDLFSIFESLENVAEFTMIEDDIEPVKDGEETTS 60

Query: 61  LVFSETELETSPKSKRLKM------TTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQ 120
           LVFSETELETSPK+KRLK       T  M+S   + SE+PNPDGPQRMSHITVERNRRKQ
Sbjct: 61  LVFSETELETSPKTKRLKTIAATSPTAAMTSTGLSSSEDPNPDGPQRMSHITVERNRRKQ 120

Query: 121 MNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK-VYSEVLSPR 180
           MNEHL+VLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK VYSEVLSPR
Sbjct: 121 MNEHLTVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVVYSEVLSPR 180

Query: 181 VVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK-PPRLQQPPSTGTAAAAAAGG 240
           VVSSPR NLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK PPRLQQPPS      AA GG
Sbjct: 181 VVSSPRPNLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPPRLQQPPS------AATGG 240

Query: 241 GGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSID------------MNANDLVA 300
           G YN+ SS+ S TTN+ NNNSLE PSPCNS S TTNSSID             N NDLVA
Sbjct: 241 GAYNIPSSSSS-TTNMANNNSLE-PSPCNSCS-TTNSSIDNNNNNNNSNSNSNNNNDLVA 300

Query: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNS 343
           NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQA KIISALEQLSLEILHVKITTLDETM NS
Sbjct: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAFKIISALEQLSLEILHVKITTLDETMFNS 356

BLAST of MS000469 vs. ExPASy TrEMBL
Match: A0A5A7U5B2 (Transcription factor SPEECHLESS OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00130 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 9.0e-137
Identity = 296/365 (81.10%), Postives = 309/365 (84.66%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVD---NPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTS 60
           MVD+ AD FQQN+EFVD   NPH EGDDLFSIFESLE+VAEFT+IEDD E  KDGEETTS
Sbjct: 1   MVDTFADLFQQNDEFVDHTLNPHFEGDDLFSIFESLENVAEFTMIEDDTEPVKDGEETTS 60

Query: 61  LVFSETELETSPKSKRLKM------TTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQ 120
           LVFSETELETSPK+KRLK       T  M+S   + SE+PNPDGPQRMSHITVERNRRKQ
Sbjct: 61  LVFSETELETSPKTKRLKTIAATSPTAAMTSTGLSSSEDPNPDGPQRMSHITVERNRRKQ 120

Query: 121 MNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK-VYSEVLSPR 180
           MNEHL+VLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK VYSEVLSPR
Sbjct: 121 MNEHLTVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVVYSEVLSPR 180

Query: 181 VVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK-PPRLQQPPSTGTAAAAAAGG 240
           VVSSPR NLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK PPRLQQ PS      AA GG
Sbjct: 181 VVSSPRPNLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPPRLQQTPS------AATGG 240

Query: 241 GGYNMVSSAGSCTTNV-NNNNSLELPSPCNSSSTTTNSSID-----------MNANDLVA 300
           G YN +SS+ S TTN+ NNNNSLE PSPCNS S TTNSSID            N NDLVA
Sbjct: 241 GAYNNISSSSSSTTNMANNNNSLE-PSPCNSCS-TTNSSIDNNNNNNSNSNSNNNNDLVA 300

Query: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNS 343
           NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQA KIISALEQLSLEILHVKITTLDETM NS
Sbjct: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAFKIISALEQLSLEILHVKITTLDETMFNS 357

BLAST of MS000469 vs. ExPASy TrEMBL
Match: A0A1S3CNN8 (transcription factor SPEECHLESS OS=Cucumis melo OX=3656 GN=LOC103503016 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 9.0e-137
Identity = 296/365 (81.10%), Postives = 309/365 (84.66%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVD---NPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTS 60
           MVD+ AD FQQN+EFVD   NPH EGDDLFSIFESLE+VAEFT+IEDD E  KDGEETTS
Sbjct: 1   MVDTFADLFQQNDEFVDHTLNPHFEGDDLFSIFESLENVAEFTMIEDDTEPVKDGEETTS 60

Query: 61  LVFSETELETSPKSKRLKM------TTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQ 120
           LVFSETELETSPK+KRLK       T  M+S   + SE+PNPDGPQRMSHITVERNRRKQ
Sbjct: 61  LVFSETELETSPKTKRLKTIAATSPTAAMTSTGLSSSEDPNPDGPQRMSHITVERNRRKQ 120

Query: 121 MNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK-VYSEVLSPR 180
           MNEHL+VLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRK VYSEVLSPR
Sbjct: 121 MNEHLTVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVVYSEVLSPR 180

Query: 181 VVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK-PPRLQQPPSTGTAAAAAAGG 240
           VVSSPR NLAMSPRKPPLSPRLNLPISPRTPQPTSPYSK PPRLQQ PS      AA GG
Sbjct: 181 VVSSPRPNLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPPRLQQTPS------AATGG 240

Query: 241 GGYNMVSSAGSCTTNV-NNNNSLELPSPCNSSSTTTNSSID-----------MNANDLVA 300
           G YN +SS+ S TTN+ NNNNSLE PSPCNS S TTNSSID            N NDLVA
Sbjct: 241 GAYNNISSSSSSTTNMANNNNSLE-PSPCNSCS-TTNSSIDNNNNNNSNSNSNNNNDLVA 300

Query: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNS 343
           NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQA KIISALEQLSLEILHVKITTLDETM NS
Sbjct: 301 NSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAFKIISALEQLSLEILHVKITTLDETMFNS 357

BLAST of MS000469 vs. ExPASy TrEMBL
Match: A0A6J1G099 (transcription factor SPEECHLESS-like OS=Cucurbita moschata OX=3662 GN=LOC111449504 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.9e-134
Identity = 281/347 (80.98%), Postives = 302/347 (87.03%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVDN--PHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSL 60
           MV SLA F Q N+EFVDN  PHL+GDDLFSIFESLESVAEF++IEDDIE  KDGEETTSL
Sbjct: 1   MVASLAQFCQPNQEFVDNTTPHLQGDDLFSIFESLESVAEFSMIEDDIEPTKDGEETTSL 60

Query: 61  VFSETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNRRKQMNEHLSV 120
           VFSETELE SPK+KRLK TTT +   A+P EE NPDG QR+SHITVERNRRKQMNEHLS+
Sbjct: 61  VFSETELEASPKTKRLKTTTTTTMAKASPLEEANPDGTQRISHITVERNRRKQMNEHLSI 120

Query: 121 LRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVSSPRAN 180
           LR+LMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAK+QRKVYSE LSPRVVSSPR N
Sbjct: 121 LRTLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKRQRKVYSEGLSPRVVSSPRPN 180

Query: 181 LAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGTAAAAAAGGGGYNMVSSA 240
           L MSPRKPPLSPRLNLPISPRTPQPTSPY+KPPRLQQPP+T  AA A++G          
Sbjct: 181 LQMSPRKPPLSPRLNLPISPRTPQPTSPYTKPPRLQQPPTTVAAATASSG---------- 240

Query: 241 GSCTTNV-NNNNSLELPSPCNSSSTTTNSSIDMN--ANDLVANSKSAIAEVEVKFTGPNV 300
             CTTN+ NNNNSLE PSPCNSSSTT +S  + N   NDLVANSKSAIAEVEVKFTGPNV
Sbjct: 241 --CTTNMANNNNSLE-PSPCNSSSTTYSSIDNANNANNDLVANSKSAIAEVEVKFTGPNV 300

Query: 301 VLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKV 343
           VLKTVSPPIPGQAVKIISALE LSLEILHVKITTLD+TM+NSFTIK+
Sbjct: 301 VLKTVSPPIPGQAVKIISALEHLSLEILHVKITTLDQTMINSFTIKI 334

BLAST of MS000469 vs. TAIR 10
Match: AT5G53210.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.1e-76
Identity = 196/369 (53.12%), Postives = 240/369 (65.04%), Query Frame = 0

Query: 1   MVDSLADFFQQNEEFVDNPHLEGDDLFSIFESLESVAEFTVIEDDIEAFKDGEETTSLVF 60
           M + + DF ++  EFVD   L GDDLF+I ESLE   E +         KDG  ++  + 
Sbjct: 1   MQEIIPDFLEEC-EFVDT-SLAGDDLFAILESLEGAGEISPTAASTP--KDGTTSSKELV 60

Query: 61  SETELE-TSPKSKRLKMTTTMSSPM------AAPSEEPNPDGPQRMSHITVERNRRKQMN 120
            + + E +SPK K+ ++ T                E+   DG Q+MSH+TVERNRRKQMN
Sbjct: 61  KDQDYENSSPKRKKQRLETRKEEDEEEEDGDGEAEEDNKQDGQQKMSHVTVERNRRKQMN 120

Query: 121 EHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLSPRVVS 180
           EHL+VLRSLMPCFYVK+GDQASIIGGVVEYI ELQQVLQSLEAKKQRK Y+EVLSPRVV 
Sbjct: 121 EHLTVLRSLMPCFYVKRGDQASIIGGVVEYISELQQVLQSLEAKKQRKTYAEVLSPRVVP 180

Query: 181 SPRAN-LAMSPRKPPLSPRLN----------LPISPRTPQPTSPY-SKPPRL---QQPPS 240
           SPR +   +SPRKPPLSPR+N           PISPRTPQPTSPY + PP+L    QPP 
Sbjct: 181 SPRPSPPVLSPRKPPLSPRINHHQIHHHLLLPPISPRTPQPTSPYRAIPPQLPLIPQPP- 240

Query: 241 TGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMN-----AN 300
                            SS  SC       +SL  P P + +S++++ S+  N      N
Sbjct: 241 -------------LRSYSSLASC-------SSLGDPPPYSPASSSSSPSVSSNHESSVIN 300

Query: 301 DLVANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDET 343
           +LVANSKSA+A+VEVKF+G NV+LKTVS  IPGQ +KII+ALE L+LEIL V I T+DET
Sbjct: 301 ELVANSKSALADVEVKFSGANVLLKTVSHKIPGQVMKIIAALEDLALEILQVNINTVDET 344

BLAST of MS000469 vs. TAIR 10
Match: AT3G06120.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.0e-28
Identity = 98/244 (40.16%), Postives = 126/244 (51.64%), Query Frame = 0

Query: 99  MSHITVERNRRKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKK 158
           MSHI VERNRR+QMNEHL  LRSL PCFY+K+GDQASIIGGV+E+IKELQQ++Q LE+KK
Sbjct: 1   MSHIAVERNRRRQMNEHLKSLRSLTPCFYIKRGDQASIIGGVIEFIKELQQLVQVLESKK 60

Query: 159 QRKVYSEVLSPRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPS 218
           +RK                              LN P  P   Q   P            
Sbjct: 61  RRKT-----------------------------LNRPSFPYDHQTIEP------------ 120

Query: 219 TGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVAN 278
                            SS G+ TT V  +  +E     N  +T+T   +    N     
Sbjct: 121 -----------------SSLGAATTRVPFSR-IE-----NVMTTSTFKEVGACCN----- 173

Query: 279 SKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSF 338
             S  A VE K +G NVVL+ VS  I GQ VKIIS LE+LS ++LH+ I++++ET+L  F
Sbjct: 181 --SPHANVEAKISGSNVVLRVVSRRIVGQLVKIISVLEKLSFQVLHLNISSMEETVLYFF 173

Query: 339 TIKV 343
            +K+
Sbjct: 241 VVKI 173

BLAST of MS000469 vs. TAIR 10
Match: AT3G24140.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-27
Identity = 101/297 (34.01%), Postives = 141/297 (47.47%), Query Frame = 0

Query: 49  FKDGEETTSLVFSETELETSPKSKRLKMTTTMSSPMAAPSEEPNPDGPQRMSHITVERNR 108
           F  GEE      + T+ E   K KR + + T        SEE      QRM+HI VERNR
Sbjct: 158 FIGGEEEDRENKNVTKKEVKSKRKRARTSKT--------SEEVE---SQRMTHIAVERNR 217

Query: 109 RKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEAKKQRKVYSEVLS 168
           RKQMNEHL VLRSLMP  YV++GDQASIIGG +E+++EL+Q+LQ LE++K+R++  E   
Sbjct: 218 RKQMNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRILGET-- 277

Query: 169 PRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQPPSTGTAAAAAAG 228
                                      ++  T   +SP +      QP           G
Sbjct: 278 ------------------------GRDMTTTTTSSSSPITTVANQAQPLIITGNVTELEG 337

Query: 229 GGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLVANSKSAIAEVEV 288
           GGG                                          +  A +KS +A+VEV
Sbjct: 338 GGG----------------------------------------LREETAENKSCLADVEV 377

Query: 289 KFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLNSFTIKVYTQ 346
           K  G + ++K +S   PGQ +K I+ALE L L ILH  ITT+++T+L SF +K+ ++
Sbjct: 398 KLLGFDAMIKILSRRRPGQLIKTIAALEDLHLSILHTNITTMEQTVLYSFNVKITSE 377

BLAST of MS000469 vs. TAIR 10
Match: AT1G72210.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.8e-17
Identity = 78/246 (31.71%), Postives = 113/246 (45.93%), Query Frame = 0

Query: 97  QRMSHITVERNRRKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEA 156
           QRM+HI VERNRRKQMNE+L+VLRSLMP +Y ++GDQASI+GG + Y+KEL+  LQS+E 
Sbjct: 123 QRMTHIAVERNRRKQMNEYLAVLRSLMPPYYAQRGDQASIVGGAINYLKELEHHLQSME- 182

Query: 157 KKQRKVYSEVLSPRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQP 216
                       P V ++     A   +    S   + P S     P   YS  P     
Sbjct: 183 ------------PPVKTATEDTGAGHDQTKTTSASSSGPFSDFFAFP--QYSNRP----- 242

Query: 217 PSTGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLV 276
               T+AAAA G                                                
Sbjct: 243 ----TSAAAAEG------------------------------------------------ 290

Query: 277 ANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLN 336
                 +AE+EV     +  LK ++   P Q +K++S+++ L L +LH+ +TT D+++L 
Sbjct: 303 ------MAEIEVTMVESHASLKILAKKRPRQLLKLVSSIQSLRLTLLHLNVTTRDDSVLY 290

Query: 337 SFTIKV 343
           S ++KV
Sbjct: 363 SISVKV 290

BLAST of MS000469 vs. TAIR 10
Match: AT1G22490.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 2.4e-17
Identity = 73/246 (29.67%), Postives = 109/246 (44.31%), Query Frame = 0

Query: 97  QRMSHITVERNRRKQMNEHLSVLRSLMPCFYVKKGDQASIIGGVVEYIKELQQVLQSLEA 156
           QRM+HI VERNRRKQMNE+L+VLRSLMP  Y ++GDQASI+GG + Y+KEL+ +LQS+E 
Sbjct: 113 QRMTHIAVERNRRKQMNEYLAVLRSLMPSSYAQRGDQASIVGGAINYVKELEHILQSMEP 172

Query: 157 KKQRKVYSEVLSPRVVSSPRANLAMSPRKPPLSPRLNLPISPRTPQPTSPYSKPPRLQQP 216
           K+                                                     R   P
Sbjct: 173 KR----------------------------------------------------TRTHDP 232

Query: 217 PSTGTAAAAAAGGGGYNMVSSAGSCTTNVNNNNSLELPSPCNSSSTTTNSSIDMNANDLV 276
               T+ ++  G                             +    +T SS D      V
Sbjct: 233 KGDKTSTSSLVG-----------------------PFTDFFSFPQYSTKSSSD------V 277

Query: 277 ANSKSAIAEVEVKFTGPNVVLKTVSPPIPGQAVKIISALEQLSLEILHVKITTLDETMLN 336
             S S+ AE+EV     +  +K ++   P Q +K+I++L+ L L +LH+ +TTL  ++L 
Sbjct: 293 PESSSSPAEIEVTVAESHANIKIMTKKKPRQLLKLITSLQSLRLTLLHLNVTTLHNSILY 277

Query: 337 SFTIKV 343
           S +++V
Sbjct: 353 SISVRV 277

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141167.13.4e-17599.42transcription factor SPEECHLESS [Momordica charantia][more]
XP_038906043.12.3e-13982.51transcription factor SPEECHLESS [Benincasa hispida][more]
XP_004149468.16.4e-13781.37transcription factor SPEECHLESS [Cucumis sativus] >KGN57858.1 hypothetical prote... [more]
XP_008465382.11.8e-13681.10PREDICTED: transcription factor SPEECHLESS [Cucumis melo] >KAA0050458.1 transcri... [more]
KAG6574226.11.0e-13480.97Transcription factor SPEECHLESS, partial [Cucurbita argyrosperma subsp. sororia]... [more]
Match NameE-valueIdentityDescription
Q700C77.2e-7553.12Transcription factor SPEECHLESS OS=Arabidopsis thaliana OX=3702 GN=SPCH PE=1 SV=... [more]
Q9M8K61.5e-2740.16Transcription factor MUTE OS=Arabidopsis thaliana OX=3702 GN=MUTE PE=2 SV=1[more]
Q56YJ82.1e-2634.01Transcription factor FAMA OS=Arabidopsis thaliana OX=3702 GN=FAMA PE=1 SV=1[more]
Q9C7T42.6e-1631.71Transcription factor bHLH96 OS=Arabidopsis thaliana OX=3702 GN=BHLH96 PE=1 SV=1[more]
Q9SK913.4e-1629.67Transcription factor bHLH94 OS=Arabidopsis thaliana OX=3702 GN=BHLH94 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CJ401.7e-17599.42transcription factor SPEECHLESS OS=Momordica charantia OX=3673 GN=LOC111011625 P... [more]
A0A0A0L7S93.1e-13781.37BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354520 PE=4 S... [more]
A0A5A7U5B29.0e-13781.10Transcription factor SPEECHLESS OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3CNN89.0e-13781.10transcription factor SPEECHLESS OS=Cucumis melo OX=3656 GN=LOC103503016 PE=4 SV=... [more]
A0A6J1G0991.9e-13480.98transcription factor SPEECHLESS-like OS=Cucurbita moschata OX=3662 GN=LOC1114495... [more]
Match NameE-valueIdentityDescription
AT5G53210.15.1e-7653.12basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G06120.11.0e-2840.16basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G24140.11.5e-2734.01basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G72210.11.8e-1731.71basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G22490.12.4e-1729.67basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 140..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..227
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..98
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 188..213
NoneNo IPR availablePANTHERPTHR46684:SF4TRANSCRIPTION FACTOR SPEECHLESScoord: 1..342
NoneNo IPR availableCDDcd11448bHLH_AtFAMA_likecoord: 99..172
e-value: 4.43095E-45
score: 147.108
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 102..153
e-value: 5.0E-12
score: 56.0
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 97..148
e-value: 4.2E-12
score: 45.9
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 96..147
score: 15.776831
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 90..161
e-value: 2.4E-14
score: 55.0
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 95..169
IPR044283Transcription factor FAMA/SPEECHLESS/MUTEPANTHERPTHR46684TRANSCRIPTION FACTOR FAMAcoord: 1..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000469.1MS000469.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010052 guard cell differentiation
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0046983 protein dimerization activity