MS000468 (gene) Bitter gourd (TR) v1

Overview


Name	MS000468
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	dihydrofolate synthetase isoform X1
Location	scaffold44: 1903615 .. 1908922 (+)
RNA-Seq Expression	MS000468
Synteny	MS000468

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTCTTCAAATATCATCGCCGTTATCGCCCACAAATTCTCGGAGGGCTTCTCCTAAATTCTTTGGTGGGTGAAGGGCCTTCTATCAGTTGCAGAATAGGGTCCAAACAATGCTTCGCCACCCACTCAGAAGATCTAGAGCTAACGGAATTCATGGGATACTTGGATTCCTTGAAGAACTACGAGAAGTTGGGTGTGCCCAGAGGCGCCGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGACTGATGGAGCGGCTGGGTAATCCGCAATCCAAGTTTAAGGTTGCCTCATTACCATGAACTTTTACTTTTCCTAGTCTTAACTAAATCTTGCTGTTTAGGACTAACCCACTTCCTCTCCAGTCCAAGGAGTTTAATTAGACTTGAATTTATTTGTTAAATCACCCCTTGACTTAGAAGCTTAGCCTGTGGTCACAGTAAATTTAAACCTTTATTGATTAACACTTTCCCCAGTGGAATTGGAAATTAGTGGAAGAAAATGACATTATACAAGGGTTCAAACTCAAGAGTATGACTCTATACCTGATACACGTTAAATCACCACTCAACCTATTAAACCTAAAAATTTAAGTTCAAGTATGTAAATTAATCATTTATTCAGGTTCTTAACCATATTAACTATTTACCTCTTAGTGACTAATGGTAGAAGAATTATAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGGTACAGCCTTGTTGGAAGATTTATCAGATTTATGCATACCCTTACTTGGTAGTGATTTAGGAAGGCATAACTTTGCGTTTGTGCATTTTTTATGTGCAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAGTTTCTTGACCAGTCAGTTGAATTTGAAAATGGGCGTATTAGTCATTTCGAGGTACTAATTCATGCAGTCTGTTGTAGTGATTGGAAAAATCTCTAAGAACCCAAATGGCTTATATGCTGCTTCTGATTCTACGTGATGTGATTAAGCAAAAGGATATTTCAGGTCTATGTTTTCGGTCGGTAAATTTGTCTGTCTCATGATCTTTTATTTCGTTCAAGATTTGAACTTCGTACATGTTTGATACCCTGCATCCTTTTGGGCTTGATTTCATGTGGGAAGGCATAAATTGTTCCAGTCAATGCTAAACCTTTCTGGGCCGTTTAAAAGTTTGTTGGACCTATGCAGTCATTAATATTTTCTTTGATCTTTTAGACGAGCTGATGAAATTCTCCAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCACAAGAGAATGTTGATGTTGCAGTCATTGAGGTTCACTTTCTTGTTGACTATTTTTTGTTTGGCAAGTATTTTTTTCTCTCTAGAACAAGCTTTCAAGGCCATTAACGATCTGTTCTAATTTTAACTGAAACAGGCTGGACTTGGTGGTGCACGAGATGCAACAAACATAATTTGCAGCTCTAAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTAGGTGGCTCTTTGGAGAGCATTGCAATGGCAAAGGCTGGAATAATTAAACACGGTTGTCCGGTTAGGTTGTTTTTTTGATGTATAATTTTGGTGTTGATTGACTTGTTCAGCATTTGATATCCATTATCCATGCATTAGTTAAGTTTTCTCTTTTCTAATGACTTTACATGATTTACAATACCAATCCAGGATTAAATATCCTTTGAGTTTTTTGGTAACCAAATGTAGTAGGTAGGGTTAGGCAGTTGTCTCGTGAGAATACTCGAGATGCACGCAAGTTGACCGGGATGCTCATAGATATAAAAAAAAAAAAAAAAAAAAGAAGAAGAAGAAGAAGAAGAGAAGAGAAGAGGGGACTTTAAGTTAGCCTGAACTTACTTAAGAGTAAGCATATCACAAAAAAAGGCTCATCATAATTGTTTAAAAGATTTGTTTTTGTTGAAAGACCAGAAGAGATACAGTACAACTAAGATGCCGCAAAACTCTCCTATCTCTCTCCACGTATATCATCAAAAACATAAATCCTATTTCTAACTTTCAAAATCTCCCTTAAAATAGCTGAGGAGGGTTTATTTGATAGTAAGATATTATTATATATATATATATATATATATCAGTGAGTGTCCGGACCAACTTGTGGCGTACCTCGAATAATTTTATGGGACAACCACCTAATCCTACTACATTTAGTTGTCAAGGTAACTCATAGGAAATATCTTACGTAGGTGGCTACCATGGTTTGAACTTATTCCCTCTAAGCCATTTACTATTTACATTGGCACTTATTTGACCACAAGATCATTCCTTGATAGAAGGATTATTAACCTATTGACCTGAATAATCTCCTACTGATGAAGGACCTATTGAATACTGATTTAACTTCCTAAAGTCTATTCCCGTAAACTCTTAGGAAAATAACAATGGAAAAACTTGATCTACATTGTTTCACTCTATTTTGTACACCAAACATCTTGCTGTACTCTATTATTCATATTAATTCTCCCGTGAACTTTGAGCATAACAAAACTGAATTGCCATGTGGACGTTATCAAATTGATTTATTGCTGTAAGCAAACAACCTGTATGAAAAGTTCTCAGAGACATCCAAAGCCCATTCTCTGGTATTTGGCCTCCAGGGGCATGGAGTTTTCTCACCTGAAGCTGTTAACCTAAATGTATGTATTTCTGTGTTTGTGTGAGAGAGAGAGAGAGAGTTGAAGAGGAGTCCATTATTGAACTCATGTGTACTAATTCTCCAACACCTATTCTGATAATATTGTCCTTCTCATTTTATCTGATTTCTCTTCTGAAATTTATTCCATGCTCCTTGCATTTGATTTCAGACTATATTGGGTGGTCCTTTCCTTCCAAGTATTGAGTGCATTCTTCGCCATAAAGCATTATCCATGTCTTCGTATGTAATATCTGCCTCATATCCTGGACATAGAAGTACTATAAAAGGTGTAAGCCTGCTTGATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAAGTATGGCTCATGTATTCTACCAAATAAATTTAAATTTCACTCCTTGAACTTCTATCAATAATTCCTAAATCTTTTTGCCAATGCAGTTCATTGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAACGCAGCAACTGCAACTTGTGCTATTCTCACTCTCCGCAATTTAGGTATAATATCATTTTTGATATTTTATTTGATCTCTGTACCTTCATTTAGAGAATAAACTATTCTAATAAATAATTCAGGTTGGAGAATTTCGGATTCCTCAATTAAGACTGGACTAGAGCAAACATTCTTGGTCGGTAGGAGCCATTTCTTATCAGCAAAGGAGGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTGCATTCATATTTCTGTTTATTACAGTCAAATTTGGTTTGTTGGGAGATGAGGGTTTTTTTTTCCTTTTTTATTTTATATATAATTTTAAATACCATGAACATCTAGCTAGCTTATGTGCATCGTGACCAGTCACAAGACAATTATTGGAGCCTACAACGTTTGGTTAGGAACATTTAATTGTCAGGAAATTCGTAGATAGATGGTTACTATAGTATTTTAACTTACCTCTTGACTACTGAGCCAACCCTTGGACGTGAGCATTGTGCTTGTGCCATGTCTCCTCTACCCTACTGTACTTGAGACTTTTGGTAATTTGATGTTTGAATCATGGGTACTCTTGTAGATGTCACCGGTACATGTTTCAAATTCATGGATTGCTAATTGCTCAAAAAACTCCAGAATGTTCAAGGTCTTTTTGGTGTTGATCAAATGGGTTTAGCTGATAAAATTTCAACCTGTGTCGGCTGATATATAGGGAAGTTTATGTTGTGTTGCTCTGGTTTAGAAGTTGGTCTCATACCTTACATTCATTTAGACACCCTTTCTGAGTGGTTTCTTGAATGTTTTCTCAGCCCACACCAAAGACTCTGCCAAAGCATTAGTGGACACCATTCAGATGACTTTTCCGGAGGCACGGTTGGCTCTCGTGGTTGCAATGGCTAGCGATAAGGATCACGTTGGTTTTGCCAGAGAATTTCTTCAAGGTATAGCCTTCCCCTTCAAAGAGTTCGTCCCAGATTTTCTTTGACCTACAGAGGACAGATTTCTTCTCTCCATTCACCTCGAAGAATATTGCTTGATTGTAAAATCTTCAATAGTCATGAAACTTTTGGATTATATAGGGTTTGTTTGATAACTATGTTACTTTTGGATCATTTTAAAATGTGTGCAGAGGTAGATTAGCTAAGGTTATAAAAAGAGCTTAACTTAGAGATAGTATGAGCAAGATAGATCAGTGAAAGAATGTGTAAAGAGAAATAGTAAGAGAAACGCTGTGTTCAAGAGTAGAAAACAGAAAATAGGTTTTTGAAATTAGTTTTTTTTTGTTCTTAATGCTAGTTTAGATTTCCAAAGTATTGTTGGAAAACAGAAAACTAAAAGCAAAAAAATGTCATCCAACATACTTTATAAAAACAAAAGCAGAAAACAGAGTTGTCTTCTTAACCTCTTTTATCAAATTACACGTTTCCTATTCTTTCAAATTGAGAATGTCAAATGTTTCTGCTCTTTCTTCATTGTCTTACTGCTTGGTGATTTAAACGTAATAATCGCTTTCTGTAGAGATTCAATTTGAATCTGGGATATCTCGACGAAAACATAACAATCTGAGTTCCCATCTGCCTTCTCTAATGTGTGAGATGTATAGCTATGAAATGCTGACAGTCGGCAGTTACTGAACTTGCAGGTGGAAAATTGGAGTCCATTATCTTGACTGAAGCTAATATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCCATTCTAAGAGACTGCTGGATCCAAGCAGCCAATGAATTGGGGATCCCAATTTCTCTAGAAACTAAAGATGCACTGGTCTCCTCCACAAGCAAACTCGAAAGCAGACCGATATTAACTACCGAGACCTCGTTATTACATGCCATGAAAATTGCTGCTGAGATTCTCAAGCAGAGAACGGAAGGGCGAGCAGGTCTTACCGTAGTAACTGGGTCGCTGCATGCTGTTTCAATGGTGTTATCTTCTCTCCATTCT

mRNA sequence

ATGAATGTCTTCAAATATCATCGCCGTTATCGCCCACAAATTCTCGGAGGGCTTCTCCTAAATTCTTTGGTGGGTGAAGGGCCTTCTATCAGTTGCAGAATAGGGTCCAAACAATGCTTCGCCACCCACTCAGAAGATCTAGAGCTAACGGAATTCATGGGATACTTGGATTCCTTGAAGAACTACGAGAAGTTGGGTGTGCCCAGAGGCGCCGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGACTGATGGAGCGGCTGGGTAATCCGCAATCCAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAGTTTCTTGACCAGTCAGTTGAATTTGAAAATGGGCGTATTAGTCATTTCGAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCACAAGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACAAACATAATTTGCAGCTCTAAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTAGGTGGCTCTTTGGAGAGCATTGCAATGGCAAAGGCTGGAATAATTAAACACGGTTGTCCGACTATATTGGGTGGTCCTTTCCTTCCAAGTATTGAGTGCATTCTTCGCCATAAAGCATTATCCATGTCTTCGTATGTAATATCTGCCTCATATCCTGGACATAGAAGTACTATAAAAGGTGTAAGCCTGCTTGATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCATTGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAACGCAGCAACTGCAACTTGTGCTATTCTCACTCTCCGCAATTTAGGTTGGAGAATTTCGGATTCCTCAATTAAGACTGGACTAGAGCAAACATTCTTGGTCGGTAGGAGCCATTTCTTATCAGCAAAGGAGGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTGCATTCATATTTCTGTTTATTACAGTCAAATTTGGTTTGTTGGGAGATGAGGCCCACACCAAAGACTCTGCCAAAGCATTAGTGGACACCATTCAGATGACTTTTCCGGAGGCACGGTTGGCTCTCGTGGTTGCAATGGCTAGCGATAAGGATCACGTTGGTTTTGCCAGAGAATTTCTTCAAGGTGGAAAATTGGAGTCCATTATCTTGACTGAAGCTAATATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCCATTCTAAGAGACTGCTGGATCCAAGCAGCCAATGAATTGGGGATCCCAATTTCTCTAGAAACTAAAGATGCACTGGTCTCCTCCACAAGCAAACTCGAAAGCAGACCGATATTAACTACCGAGACCTCGTTATTACATGCCATGAAAATTGCTGCTGAGATTCTCAAGCAGAGAACGGAAGGGCGAGCAGGTCTTACCGTAGTAACTGGGTCGCTGCATGCTGTTTCAATGGTGTTATCTTCTCTCCATTCT

Coding sequence (CDS)

ATGAATGTCTTCAAATATCATCGCCGTTATCGCCCACAAATTCTCGGAGGGCTTCTCCTAAATTCTTTGGTGGGTGAAGGGCCTTCTATCAGTTGCAGAATAGGGTCCAAACAATGCTTCGCCACCCACTCAGAAGATCTAGAGCTAACGGAATTCATGGGATACTTGGATTCCTTGAAGAACTACGAGAAGTTGGGTGTGCCCAGAGGCGCCGGGACGGATTCTGATGATGGGTTCGATCTCGGAAGGATGAGAAGACTGATGGAGCGGCTGGGTAATCCGCAATCCAAGTTTAAGGCCATTCACATTGCGGGAACCAAGGGAAAAGGGTCGACTGCTGCCTTCCTGTCTAACATTTTACGAGCAGAGGGATACTCTGTTGGCTGTTATACTAGTCCTCATATTGAAACTATAAGGGAACGTATTTCACTCGGAAGATCTGGAGAGATGGTCTCTGGGAAGGCACTAAACTCTCTTTTCAAAAGGAACAAAGAGTTTCTTGACCAGTCAGTTGAATTTGAAAATGGGCGTATTAGTCATTTCGAGGTCCTTACTGCTATGGCATTCTCACTCTTTGCACAAGAGAATGTTGATGTTGCAGTCATTGAGGCTGGACTTGGTGGTGCACGAGATGCAACAAACATAATTTGCAGCTCTAAACTTGCTGCTGCAGTCATAACTTCAATTGGAGAGGAACACATGGCTGCCCTAGGTGGCTCTTTGGAGAGCATTGCAATGGCAAAGGCTGGAATAATTAAACACGGTTGTCCGACTATATTGGGTGGTCCTTTCCTTCCAAGTATTGAGTGCATTCTTCGCCATAAAGCATTATCCATGTCTTCGTATGTAATATCTGCCTCATATCCTGGACATAGAAGTACTATAAAAGGTGTAAGCCTGCTTGATGGAAGACTTTGCCAATGCTGCGACATAGTAATTCAAATAGACAATGAATTCATTGAGTTATTGGATGTCAACCTCCGCATGCTTGGACGTCATCAACTTCAGAACGCAGCAACTGCAACTTGTGCTATTCTCACTCTCCGCAATTTAGGTTGGAGAATTTCGGATTCCTCAATTAAGACTGGACTAGAGCAAACATTCTTGGTCGGTAGGAGCCATTTCTTATCAGCAAAGGAGGCTGGGGTTCTTGGACTACCTGGAGCAACAATATTGCTGGATGGAGGTGCATTCATATTTCTGTTTATTACAGTCAAATTTGGTTTGTTGGGAGATGAGGCCCACACCAAAGACTCTGCCAAAGCATTAGTGGACACCATTCAGATGACTTTTCCGGAGGCACGGTTGGCTCTCGTGGTTGCAATGGCTAGCGATAAGGATCACGTTGGTTTTGCCAGAGAATTTCTTCAAGGTGGAAAATTGGAGTCCATTATCTTGACTGAAGCTAATATTGGTGGAGGCAAATCAAGGACAACTTCAGCTGCCATTCTAAGAGACTGCTGGATCCAAGCAGCCAATGAATTGGGGATCCCAATTTCTCTAGAAACTAAAGATGCACTGGTCTCCTCCACAAGCAAACTCGAAAGCAGACCGATATTAACTACCGAGACCTCGTTATTACATGCCATGAAAATTGCTGCTGAGATTCTCAAGCAGAGAACGGAAGGGCGAGCAGGTCTTACCGTAGTAACTGGGTCGCTGCATGCTGTTTCAATGGTGTTATCTTCTCTCCATTCT

Protein sequence

MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQRTEGRAGLTVVTGSLHAVSMVLSSLHS

Homology

[Close]

BLAST of MS000468 vs. NCBI nr
Match: XP_022141068.1 (dihydrofolate synthetase isoform X1 [Momordica charantia])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.3e-300
Identity = 543/566 (95.94%), Postives = 544/566 (96.11%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MNVFKYHRRYRPQILGGLLLN LVGEGPSISCRIGSKQCFATHSEDLELTEFM YLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPFLPSIECIL  KALSMSSYVISASYPGHRSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDG                  AHTKDSA
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS
Sbjct: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR
Sbjct: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
           TEGRAGLTVVTGSLHAVSMVLSSLHS
Sbjct: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of MS000468 vs. NCBI nr
Match: XP_023553540.1 (dihydrofolate synthetase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 944.1 bits (2439), Expect = 5.5e-271
Identity = 487/566 (86.04%), Postives = 519/566 (91.70%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MN+FKYH  +RPQI G LLLN  VGEGPSIS RIGSKQCF  HSED ++TEFM YLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLS+IL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSSIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECILR KALSMSS V+SAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG TILLDG                  AHTKDSA
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQM+FP+A+LALVVAMASDKDH GFA EFLQGGKLESI+L+EANIGGGKSRTTS
Sbjct: 421 KALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQGGKLESIVLSEANIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AA+LRDCWIQA+NE+GIPISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR
Sbjct: 481 AALLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
            EGR GL VVTGSLHAVSMVLSSLHS
Sbjct: 541 IEGRRGLVVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of MS000468 vs. NCBI nr
Match: KAG6576673.1 (Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 941.4 bits (2432), Expect = 3.6e-270
Identity = 484/566 (85.51%), Postives = 518/566 (91.52%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MN+FKYH  +RPQI G LLLN  VGEGPSIS RIGSKQCF  HSED ++TEFM YLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGT+S+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTESEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECILR KALSMSS V+SAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG  ILLDG                  AHTKDSA
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQM+FP+A+LALVVAMASDKDH GFA EFLQGGKLESI+L+EANIGGGKSRTTS
Sbjct: 421 KALVDTIQMSFPDAQLALVVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AA+LRDCWIQA+NE+GIPISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR
Sbjct: 481 AALLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
            +GR GL VVTGSLHAVSMVLSSLHS
Sbjct: 541 IDGRRGLVVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of MS000468 vs. NCBI nr
Match: XP_022922898.1 (dihydrofolate synthetase isoform X1 [Cucurbita moschata])

HSP 1 Score: 938.7 bits (2425), Expect = 2.3e-269
Identity = 483/566 (85.34%), Postives = 517/566 (91.34%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MN+FKYH  +RPQI G LLLN  VGEGPSIS RIGSKQCF  HSED ++TEFM YLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECILR KALSMSS V+SAS PG+RS+IKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG  ILLDG                  AHTKDSA
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQM+FP+A+LALVVAMASDKDH GFA EFLQGGKLESI+L+EANIGG KSRTTS
Sbjct: 421 KALVDTIQMSFPDAQLALVVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AA+LRDCWIQA+NE+GIPISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR
Sbjct: 481 AALLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
            +GR GL VVTGSLHAVSMVLSSLHS
Sbjct: 541 IDGRRGLVVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of MS000468 vs. NCBI nr
Match: XP_038905573.1 (dihydrofolate synthetase [Benincasa hispida])

HSP 1 Score: 933.3 bits (2411), Expect = 9.7e-268
Identity = 487/566 (86.04%), Postives = 509/566 (89.93%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           M +FKYH   RPQILG LLLN   GE PSIS RIGSKQCF+TH ED  +TEFM YLDSLK
Sbjct: 1   MKIFKYHHHCRPQILGTLLLNHFRGECPSISVRIGSKQCFSTHPEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP+GAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPKGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYS GCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEF DQSVE ENGRISH
Sbjct: 121 RAEGYSFGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFFDQSVELENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAF LFAQENVD+AVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFLLFAQENVDLAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIA AKAGIIK GCP ILGGPFLPSIE ILR KALSMSS VISAS PG+RSTIKGVSL
Sbjct: 241 LESIATAKAGIIKRGCPAILGGPFLPSIERILRDKALSMSSPVISASGPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+G +CQCCD+VIQID+EFIELLDVNLRMLG HQLQNAATATC IL LRNLGWRISD+S+
Sbjct: 301 LNGGICQCCDVVIQIDDEFIELLDVNLRMLGHHQLQNAATATCVILALRNLGWRISDASV 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLEQTFLVGRSHFL AKEAGVLGLPGATILLDG                  AHTKDSA
Sbjct: 361 RSGLEQTFLVGRSHFLPAKEAGVLGLPGATILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQM FPEARLALVVAMASDKDH+GFAREFLQGGKLESI+LTEA+IGGGKSRTTS
Sbjct: 421 KALVDTIQMAFPEARLALVVAMASDKDHIGFAREFLQGGKLESIVLTEAHIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           A  LRDCWIQA+NELGIPISLETKDA VS TSKLE+RP+LTTETSLLHA+K+AAEILKQR
Sbjct: 481 AVFLRDCWIQASNELGIPISLETKDAPVSFTSKLENRPVLTTETSLLHAIKVAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
           TEGR GL VVTGSLHAVSMVLS LHS
Sbjct: 541 TEGRQGLVVVTGSLHAVSMVLSFLHS 548

[Close]

BLAST of MS000468 vs. ExPASy Swiss-Prot
Match: F4JYE9 (Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.3e-158
Identity = 317/528 (60.04%), Postives = 386/528 (73.11%), Query Frame = 0


Query: 44  SEDLELTEFMGYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED EL +F+G+L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPFLP IE ILR KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 ISASYPGHRSTIKGVSLLDG-RLCQCCDIVIQ---IDNEFIELLDVNLRMLGRHQLQNAA 343
           I AS  G  S+IKG+   +G  LCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCAILTLRNLG-WRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAF 403
           TATC  L LR+ G  R++D +I+ GLE T L+GRS FL+ KEA  L LPGAT+LLDG   
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDG--- 399

Query: 404 IFLFITVKFGLLGDEAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQG 463
                          AHTK+SA+AL + I+  FPE RL  VVAMASDKDHV FA+E L G
Sbjct: 400 ---------------AHTKESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSG 459

Query: 464 GKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLETKDALVSSTSKLESRP 523
            K E++ILTEA+IGGGK R+T +++L++ WI+AA+ELG                   SR 
Sbjct: 460 LKPEAVILTEADIGGGKIRSTESSVLKESWIKAADELG-------------------SRS 519

Query: 524 ILTTET-SLLHAMKIAAEILK-QRTEGRAGLTVVTGSLHAVSMVLSSL 565
           +  +E  ++L ++K+A +IL    T   +G+ +VTGSLH VS VL+SL
Sbjct: 520 MEASENKTVLGSLKLAYKILSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of MS000468 vs. ExPASy Swiss-Prot
Match: Q05865 (Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=folC PE=3 SV=2)

HSP 1 Score: 168.7 bits (426), Expect = 1.9e-40
Identity = 121/371 (32.61%), Postives = 190/371 (51.21%), Query Frame = 0


Query: 81  LGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRE 140
           LGRM++LM RLG+P+ K +A H+AGT GKGST AF+ ++L+  GY+VG +TSP+I T  E
Sbjct: 24  LGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNE 83

Query: 141 RISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQ-ENVDV 200
           RIS+  +G  +S +   +L  + K  ++   + E G+ + FE++TA AF  FA+   VD 
Sbjct: 84  RISV--NGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDF 143

Query: 201 AVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTI 260
            + E GLGG  D+TN++   +    VITSIG +HM  LG ++E IA  KAGIIK G P I
Sbjct: 144 VIFETGLGGRFDSTNVV---EPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIP-I 203

Query: 261 LGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQIDNEF 320
           +     P    ++RH+A   ++       P        V   +  L        + + + 
Sbjct: 204 VTAVTQPEALQVIRHEAERHAA-------PFQSLHDACVIFNEEALPAGEQFSFKTEEKC 263

Query: 321 IELLDVNLRMLGRHQLQNAATATCAI--LTLRNLGWRISDSSIKTGLEQTFLVGRSHFLS 380
            E  D+   ++G HQ QNAA +  A   L   N+   ISD ++++GL +    GR   + 
Sbjct: 264 YE--DIRTSLIGTHQRQNAALSILAAEWLNKENIA-HISDEALRSGLVKAAWPGRLELVQ 323

Query: 381 AKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSAKALVDTIQMTFPEARLA 440
                        + LDG                  AH ++  + L +T++  F  +R++
Sbjct: 324 EH---------PPVYLDG------------------AHNEEGVEKLAETMKQRFANSRIS 351

Query: 441 LVVAMASDKDH 449
           +V +   DK +
Sbjct: 384 VVFSALKDKPY 351

[Close]

BLAST of MS000468 vs. ExPASy Swiss-Prot
Match: P15925 (Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 9.8e-29
Identity = 112/419 (26.73%), Postives = 183/419 (43.68%), Query Frame = 0


Query: 47  LELTEFMGYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGT 106
           +  TE + Y+ S     K G             D  R+  L+  LGNPQ + + IH+ GT
Sbjct: 1   MNYTETVAYIHSFPRLAKTG-------------DHRRILTLLHALGNPQQQGRYIHVTGT 60

Query: 107 KGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEF 166
            GKGS A  ++++L A G +VG YTSP I    ERI +    E +   AL +     +  
Sbjct: 61  NGKGSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDH--EPIPDAALVNAVAFVRAA 120

Query: 167 LDQSVEFE-NGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAV 226
           L++  + + +  ++ FE +TA+ +  F Q  VDVAVIE G+GG  D+TN+I       +V
Sbjct: 121 LERLQQQQADFNVTEFEFITALGYWYFRQRQVDVAVIEVGIGGDTDSTNVITP---VVSV 180

Query: 227 ITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVI- 286
           +T +  +H   LG ++ +IA  KAGIIK G P + G   +P    ++  K  +  S  + 
Sbjct: 181 LTEVALDHQKLLGHTITAIAKHKAGIIKRGIPVVTGN-LVPDAAAVVAAKVATTGSQWLR 240

Query: 287 ---SASYP-------GHRSTIKGVSLLDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQ 346
                S P       G R T +     DGR+                  D+ + ++G +Q
Sbjct: 241 FDRDFSVPKAKLHGWGQRFTYED---QDGRIS-----------------DLEVPLVGDYQ 300

Query: 347 LQNAATA-TCAILTLRNLGWRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILL 406
            +N A A   A +  +   W ++  +I+ GL  +    R   +S             I++
Sbjct: 301 QRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARLEKIS---------DTPLIVI 352

Query: 407 DGGAFIFLFITVKFGLLGDEAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFA 453
           DG                  AH  D    L+  ++  F +  + ++  + +DKD+   A
Sbjct: 361 DG------------------AHNPDGINGLITALKQLFSQP-ITVIAGILADKDYAAMA 352

[Close]

BLAST of MS000468 vs. ExPASy Swiss-Prot
Match: D4GW05 (Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=folCP PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.4e-27
Identity = 116/402 (28.86%), Postives = 177/402 (44.03%), Query Frame = 0


Query: 84  MRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERIS 143
           +RRL+  LG+P      + +AG+ GKGSTA  +  +LR  G  VG YTSPH + +RER+ 
Sbjct: 25  IRRLLSHLGDPHEGVSFVQVAGSNGKGSTARMVDAMLRESGAHVGLYTSPHFDDVRERVR 84

Query: 144 LGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIE 203
           +   G  +   AL++     K +L +    +   ++ FE +TA+A   F +  VDVAV+E
Sbjct: 85  V--DGRKIPKSALSAFVAEAKPYLVERAA-DGEPLTFFETVTALALWYFDRAGVDVAVLE 144

Query: 204 AGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILG-- 263
            G+GG  DAT+ +      A+ +T++  EH A LG ++  IA  KA +     P + G  
Sbjct: 145 VGMGGELDATSAV---DPVASAVTNVSLEHTAVLGDTVAEIAKTKAAVAPADAPLVTGTT 204

Query: 264 GPFLPSI-----ECILRHKALSMSSYVISASYPGHRSTIKGVSLLDGRLCQCCDIVIQID 323
           G  L  I     + +    A + S   +  SY G  +             Q   + ++ D
Sbjct: 205 GEALSVIREEAGDVLTVGDADADSDADVRVSYGGRVNH------------QEAAVTVETD 264

Query: 324 NEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSIKTGLEQTFLVGRSHFL 383
               E LDV + +LG +Q +NA     A+   R +   I   +I  GL      GR   +
Sbjct: 265 ---AETLDVRIPLLGAYQARNAG---IAVSLARQVRPDIDAEAIHRGLRNAHWPGRFEVM 324

Query: 384 SAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSAKALVDTIQMTFPEARL 443
             +          T++LDG                  AH  D A A V T+   F    L
Sbjct: 325 GTE---------PTVVLDG------------------AHNPD-ACAQVATVLDEFDYDDL 368

Query: 444 ALVVAMASDKDH---VGFAREFLQGGKLESIILTEANIGGGK 476
            LV     DKDH   VG   E      + S++  +A+I  G+
Sbjct: 385 HLVYGAMHDKDHGEMVGALPE------VASVVTCKADISRGE 368

[Close]

BLAST of MS000468 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.6e-26
Identity = 87/241 (36.10%), Postives = 124/241 (51.45%), Query Frame = 0


Query: 25  GEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK---NYEKLGVPRGAGTDSDDGFDL 84
           G    ++ R G          D+E  + +  L++L+   NY ++ V R  G   D    L
Sbjct: 18  GATTRVAVRRGLSAWPVLQEPDMEYQDAVRTLNTLQTNANYLEM-VKRKRG---DPQTQL 77

Query: 85  GRMRRLMERLG---NPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETI 144
             M+  + R G       +   IHI GTKGKGST AF   ILR+ G   G ++SPH+  +
Sbjct: 78  EAMKLYLARSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQV 137

Query: 145 RERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFEN--GRISHFEVLTAMAFSLFAQEN 204
           RERI +  +G+ +S +     F R    L+++ +  +      +F  LT MAF +F QE 
Sbjct: 138 RERIRI--NGQPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEK 197

Query: 205 VDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGC 258
           VD+AV+E G+GGA D TNII   K     ITS+G +H   LG ++E IA  K GI K G 
Sbjct: 198 VDLAVLEVGIGGAYDCTNII--RKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGV 250

[Close]

BLAST of MS000468 vs. ExPASy TrEMBL
Match: A0A6J1CJG1 (dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011557 PE=3 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 2.1e-300
Identity = 543/566 (95.94%), Postives = 544/566 (96.11%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MNVFKYHRRYRPQILGGLLLN LVGEGPSISCRIGSKQCFATHSEDLELTEFM YLDSLK
Sbjct: 1   MNVFKYHRRYRPQILGGLLLNFLVGEGPSISCRIGSKQCFATHSEDLELTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPFLPSIECIL  KALSMSSYVISASYPGHRSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILHDKALSMSSYVISASYPGHRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI
Sbjct: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDG                  AHTKDSA
Sbjct: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS
Sbjct: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR
Sbjct: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
           TEGRAGLTVVTGSLHAVSMVLSSLHS
Sbjct: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of MS000468 vs. ExPASy TrEMBL
Match: A0A6J1E819 (dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430739 PE=3 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 1.1e-269
Identity = 483/566 (85.34%), Postives = 517/566 (91.34%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MN+FKYH  +RPQI G LLLN  VGEGPSIS RIGSKQCF  HSED ++TEFM YLDSLK
Sbjct: 1   MNLFKYHHHFRPQIHGRLLLNYFVGEGPSISSRIGSKQCFRNHSEDQDMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSRLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECILR KALSMSS V+SAS PG+RS+IKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSSIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ DNEFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRIS++SI
Sbjct: 301 LNGRLCQCCDLIIQTDNEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISEASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG  ILLDG                  AHTKDSA
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGILGLPGTAILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQM+FP+A+LALVVAMASDKDH GFA EFLQGGKLESI+L+EANIGG KSRTTS
Sbjct: 421 KALVDTIQMSFPDAQLALVVAMASDKDHYGFATEFLQGGKLESIVLSEANIGGSKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AA+LRDCWIQA+NE+GIPISLETKDA VSSTSKLE+RP+LTTETSLL A+KIAAEILKQR
Sbjct: 481 AALLRDCWIQASNEMGIPISLETKDAPVSSTSKLENRPVLTTETSLLRAIKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
            +GR GL VVTGSLHAVSMVLSSLHS
Sbjct: 541 IDGRRGLVVVTGSLHAVSMVLSSLHS 548

[Close]

BLAST of MS000468 vs. ExPASy TrEMBL
Match: A0A1S3CNR7 (dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.2e-265
Identity = 483/566 (85.34%), Postives = 510/566 (90.11%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MNVFKYH   RPQILG LLLN  +GE PSIS R GSKQCFATHSED  +TEFM YLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRNGSKQCFATHSEDQHMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGS AAFLSNIL
Sbjct: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSIAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIET+RERISLGRSGEMVSGKALNSLFKRNKE  DQSVE E+GRISH
Sbjct: 121 RAEGYSVGCYTSPHIETLRERISLGRSGEMVSGKALNSLFKRNKEVFDQSVELEHGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSGLAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIK GCPTILGG FLP IE ILR KALSMSS VI+ S PG+RSTIKGV++
Sbjct: 241 LESIAMAKAGIIKRGCPTILGGSFLPRIEYILRDKALSMSSPVIAVSDPGNRSTIKGVNM 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+G LCQCCDIVIQIDNEF+ELLDVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGGLCQCCDIVIQIDNEFVELLDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLEQTFL+GRSHFL+AKEA VLGLPGATILLDG                  AHTKDSA
Sbjct: 361 RSGLEQTFLIGRSHFLAAKEAEVLGLPGATILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQM FPEARLALV+AMASDK+HV FAREFLQGGKLE ++LTEA+IGGGKSRTTS
Sbjct: 421 KALVDTIQMAFPEARLALVIAMASDKNHVDFAREFLQGGKLECVVLTEAHIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AA LRDCWIQA+ ELGIPISLETKDA VS TSKLE+RP+LTTETSLLHA+KIAAEILKQR
Sbjct: 481 AAFLRDCWIQASIELGIPISLETKDAEVSFTSKLENRPVLTTETSLLHAIKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
           T+G+  L VV+GSLHAVSMVLSSLHS
Sbjct: 541 TKGQQSLVVVSGSLHAVSMVLSSLHS 548

[Close]

BLAST of MS000468 vs. ExPASy TrEMBL
Match: A0A0A0L7A4 (Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G354540 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.6e-263
Identity = 479/566 (84.63%), Postives = 510/566 (90.11%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MNVFKYH   RPQILG LLLN  +GE PSIS R GSKQCFATHSED  +T+FM YLDSLK
Sbjct: 1   MNVFKYHHHCRPQILGSLLLNHFIGECPSISSRFGSKQCFATHSEDQHMTQFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVPRG+GTDSDDGFDLGRMRRLMERLGNPQS+FKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPRGSGTDSDDGFDLGRMRRLMERLGNPQSRFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           R EGYSVGCYTSPHIETIRERISLGRSG+MVSGKALNSLFKRNKE  DQSVE ENG +SH
Sbjct: 121 RVEGYSVGCYTSPHIETIRERISLGRSGDMVSGKALNSLFKRNKEVFDQSVELENGHLSH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQE+VDVAVIEAGLGGARDATNIICSS+LAAAVITSIGEEH+AALGGS
Sbjct: 181 FEVLTAMAFSLFAQEDVDVAVIEAGLGGARDATNIICSSELAAAVITSIGEEHVAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIA AKAGIIK GCPTILGGPFLP IE ILR KALSMSS VISAS PG+RSTIKGV+L
Sbjct: 241 LESIATAKAGIIKRGCPTILGGPFLPRIEYILRDKALSMSSPVISASDPGNRSTIKGVNL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+G L QCCDIVIQIDNEFIELLDVNLRMLG HQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGGLSQCCDIVIQIDNEFIELLDVNLRMLGPHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLEQTFL+GRSHFL+A+EA VLGLPGATILLDG                  AHTKDSA
Sbjct: 361 RSGLEQTFLIGRSHFLAAREAEVLGLPGATILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KAL+DTIQM FPEA+LALVVAMASDK+HVGFAREFLQGGKLES++LTEA IGGGKSRTTS
Sbjct: 421 KALLDTIQMAFPEAQLALVVAMASDKNHVGFAREFLQGGKLESVVLTEALIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AA LRDCWIQA+NELGIPISLETKDA V  TSKL +RP+LTTETSLLHA+KIAAEILKQR
Sbjct: 481 AAFLRDCWIQASNELGIPISLETKDAEVFFTSKLGNRPVLTTETSLLHAIKIAAEILKQR 540

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
           T+GR GL VV+GSLHAVSMVL+SLHS
Sbjct: 541 TKGRQGLVVVSGSLHAVSMVLASLHS 548

[Close]

BLAST of MS000468 vs. ExPASy TrEMBL
Match: A0A6J1J6Z7 (dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 9.8e-258
Identity = 472/566 (83.39%), Postives = 502/566 (88.69%), Query Frame = 0


Query: 1   MNVFKYHRRYRPQILGGLLLNSLVGEGPSISCRIGSKQCFATHSEDLELTEFMGYLDSLK 60
           MNVFKYH  +R QILG LLLN  VGEGPSIS RIGSKQCF  HSED ++TEFM YLDSLK
Sbjct: 1   MNVFKYHHHFRLQILGRLLLNYFVGEGPSISSRIGSKQCFGNHSEDQQMTEFMEYLDSLK 60

Query: 61  NYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120
           NYEKLGVP GAGTDS+DGFDLGRM+RLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL
Sbjct: 61  NYEKLGVPTGAGTDSEDGFDLGRMKRLMERLGNPQSKFKAIHIAGTKGKGSTAAFLSNIL 120

Query: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRNKEFLDQSVEFENGRISH 180
           RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALN LFKRNKE LDQSV+ ENGRISH
Sbjct: 121 RAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNFLFKRNKELLDQSVKLENGRISH 180

Query: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAAAVITSIGEEHMAALGGS 240
           FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSS LAAAVITSIGEEHMAALGGS
Sbjct: 181 FEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSILAAAVITSIGEEHMAALGGS 240

Query: 241 LESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYVISASYPGHRSTIKGVSL 300
           LESIAMAKAGIIKHGCPTILGGPF+P+IECILR KALSMSS V+SAS PG+RSTIKGVSL
Sbjct: 241 LESIAMAKAGIIKHGCPTILGGPFIPNIECILRDKALSMSSPVVSASDPGNRSTIKGVSL 300

Query: 301 LDGRLCQCCDIVIQIDNEFIELLDVNLRMLGRHQLQNAATATCAILTLRNLGWRISDSSI 360
           L+GRLCQCCD++IQ D+EFIEL DVNLRMLGRHQLQNAATATC ILTLRNLGWRISD+SI
Sbjct: 301 LNGRLCQCCDLIIQTDDEFIELFDVNLRMLGRHQLQNAATATCVILTLRNLGWRISDASI 360

Query: 361 KTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAFIFLFITVKFGLLGDEAHTKDSA 420
           ++GLE+TFLVGRSHFLSAKEAG+LGLPG TILLDG                  AHTKDSA
Sbjct: 361 RSGLEKTFLVGRSHFLSAKEAGMLGLPGTTILLDG------------------AHTKDSA 420

Query: 421 KALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQGGKLESIILTEANIGGGKSRTTS 480
           KALVDTIQM+FP+A+LALVVAMASDKDH GFA EFLQ GKLESI+L+EANIGGGKSRTTS
Sbjct: 421 KALVDTIQMSFPDAQLALVVAMASDKDHNGFATEFLQCGKLESIVLSEANIGGGKSRTTS 480

Query: 481 AAILRDCWIQAANELGIPISLETKDALVSSTSKLESRPILTTETSLLHAMKIAAEILKQR 540
           AA+LRDCWIQA+NE+GIP          + TS      +LTTETSLL A+KIAAEILKQR
Sbjct: 481 AALLRDCWIQASNEMGIP----------TQTS------LLTTETSLLRAIKIAAEILKQR 532

Query: 541 TEGRAGLTVVTGSLHAVSMVLSSLHS 567
           TEGR GL VVTGSLHAVSMVLSSLHS
Sbjct: 541 TEGRRGLVVVTGSLHAVSMVLSSLHS 532

[Close]

BLAST of MS000468 vs. TAIR 10
Match: AT5G41480.1 (Folylpolyglutamate synthetase family protein )

HSP 1 Score: 561.2 bits (1445), Expect = 9.3e-160
Identity = 317/528 (60.04%), Postives = 386/528 (73.11%), Query Frame = 0


Query: 44  SEDLELTEFMGYLDSLKNYEKLGVPRGAGTDSDDGFDLGRMRRLMERLGNPQSKFKAIHI 103
           +ED EL +F+G+L+SLKNYEK GVP+GAGTDSDDGFDLGRM+RLM RL NP  K+K +H+
Sbjct: 40  TEDPELRDFVGFLESLKNYEKSGVPKGAGTDSDDGFDLGRMKRLMLRLRNPHYKYKVVHV 99

Query: 104 AGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKALNSLFKRN 163
           AGTKGKGST+AFLSNILRA GYSVGCY+SPHI +I+ERIS   +GE VS   LN LF   
Sbjct: 100 AGTKGKGSTSAFLSNILRAGGYSVGCYSSPHILSIKERISC--NGEPVSASTLNDLFYSV 159

Query: 164 KEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNIICSSKLAA 223
           K  L+QS++ ENG +SHFE+LT +AFSLF +ENVD+AVIEAGLGGARDATN+I SS LAA
Sbjct: 160 KPILEQSIQEENGSLSHFEILTGIAFSLFEKENVDIAVIEAGLGGARDATNVIESSNLAA 219

Query: 224 AVITSIGEEHMAALGGSLESIAMAKAGIIKHGCPTILGGPFLPSIECILRHKALSMSSYV 283
           +VIT+IGEEHMAALGGSLESIA AK+GIIKHG P +LGGPFLP IE ILR KA S+SS V
Sbjct: 220 SVITTIGEEHMAALGGSLESIAEAKSGIIKHGRPVVLGGPFLPHIEGILRSKAASVSSSV 279

Query: 284 ISASYPGHRSTIKGVSLLDG-RLCQCCDIVIQ---IDNEFIELLDVNLRMLGRHQLQNAA 343
           I AS  G  S+IKG+   +G  LCQ CDIVIQ    D   +EL DVNLRMLG HQLQNA 
Sbjct: 280 ILASNIGSSSSIKGIINKNGIGLCQSCDIVIQNEKDDQPIVELSDVNLRMLGHHQLQNAV 339

Query: 344 TATCAILTLRNLG-WRISDSSIKTGLEQTFLVGRSHFLSAKEAGVLGLPGATILLDGGAF 403
           TATC  L LR+ G  R++D +I+ GLE T L+GRS FL+ KEA  L LPGAT+LLDG   
Sbjct: 340 TATCVSLCLRDQGCGRVTDEAIRIGLENTRLLGRSQFLTPKEAETLLLPGATVLLDG--- 399

Query: 404 IFLFITVKFGLLGDEAHTKDSAKALVDTIQMTFPEARLALVVAMASDKDHVGFAREFLQG 463
                          AHTK+SA+AL + I+  FPE RL  VVAMASDKDHV FA+E L G
Sbjct: 400 ---------------AHTKESARALKEMIKKDFPEKRLVFVVAMASDKDHVSFAKELLSG 459

Query: 464 GKLESIILTEANIGGGKSRTTSAAILRDCWIQAANELGIPISLETKDALVSSTSKLESRP 523
            K E++ILTEA+IGGGK R+T +++L++ WI+AA+ELG                   SR 
Sbjct: 460 LKPEAVILTEADIGGGKIRSTESSVLKESWIKAADELG-------------------SRS 519

Query: 524 ILTTET-SLLHAMKIAAEILK-QRTEGRAGLTVVTGSLHAVSMVLSSL 565
           +  +E  ++L ++K+A +IL    T   +G+ +VTGSLH VS VL+SL
Sbjct: 520 MEASENKTVLGSLKLAYKILSDDTTSSDSGMVIVTGSLHIVSSVLASL 528

[Close]

BLAST of MS000468 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 113.6 bits (283), Expect = 5.1e-25
Identity = 69/162 (42.59%), Postives = 91/162 (56.17%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E VDV ++E GLGG  DATN+
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNV 175

Query: 216 ICSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCP 258
           I   K     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 176 I--QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP 213

[Close]

BLAST of MS000468 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 112.1 bits (279), Expect = 1.5e-24
Identity = 69/162 (42.59%), Postives = 90/162 (55.56%), Query Frame = 0


Query: 96  SKFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKA 155
           S+ K IH+AGTKGKGST  F  +ILR  G   G +TSPH+  +RER  L  +G  +S + 
Sbjct: 56  SQMKIIHVAGTKGKGSTCTFAESILRCYGLRTGLFTSPHLIDVRERFRL--NGIEISQEK 115

Query: 156 LNSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNI 215
             + F      L +    E    ++F  L  +AF +F  E VDV ++E GLGG  DATNI
Sbjct: 116 FVNYFWCCFHKLKEKTSNEVPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNI 175

Query: 216 ICSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCP 258
               K     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 176 ---QKPVVCGISSLGYDHMEILGYTLAEIAAEKAGIFKSGVP 212

[Close]

BLAST of MS000468 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 111.3 bits (277), Expect = 2.5e-24
Identity = 67/161 (41.61%), Postives = 88/161 (54.66%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 111 KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 170

Query: 157 NSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNII 216
              F      L +    E    ++F  L  +AF +FA E VD A++E GLGG  DATN +
Sbjct: 171 LGYFWWCYNRLKERTNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAV 230

Query: 217 CSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCP 258
              K     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 231 --QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 267

[Close]

BLAST of MS000468 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 111.3 bits (277), Expect = 2.5e-24
Identity = 67/161 (41.61%), Postives = 88/161 (54.66%), Query Frame = 0


Query: 97  KFKAIHIAGTKGKGSTAAFLSNILRAEGYSVGCYTSPHIETIRERISLGRSGEMVSGKAL 156
           K   IH+AGTKGKGST  F  +I+R  G+  G +TSPH+  +RER  L   G  +S +  
Sbjct: 53  KMNVIHVAGTKGKGSTCTFTESIIRNYGFRTGLFTSPHLIDVRERFRL--DGVDISEEKF 112

Query: 157 NSLFKRNKEFLDQSVEFENGRISHFEVLTAMAFSLFAQENVDVAVIEAGLGGARDATNII 216
              F      L +    E    ++F  L  +AF +FA E VD A++E GLGG  DATN +
Sbjct: 113 LGYFWWCYNRLKERTNEEIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAV 172

Query: 217 CSSKLAAAVITSIGEEHMAALGGSLESIAMAKAGIIKHGCP 258
              K     I+S+G +HM  LG +L  IA  KAGI K G P
Sbjct: 173 --QKPVVCGISSLGYDHMEILGDTLGKIAGEKAGIFKLGVP 209

The following BLAST results are available for this feature:

BLAST of MS000468 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141068.1	4.3e-300	95.94	dihydrofolate synthetase isoform X1 [Momordica charantia]	[more]
XP_023553540.1	5.5e-271	86.04	dihydrofolate synthetase [Cucurbita pepo subsp. pepo]	[more]
KAG6576673.1	3.6e-270	85.51	Dihydrofolate synthetase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922898.1	2.3e-269	85.34	dihydrofolate synthetase isoform X1 [Cucurbita moschata]	[more]
XP_038905573.1	9.7e-268	86.04	dihydrofolate synthetase [Benincasa hispida]	[more]

BLAST of MS000468 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JYE9	1.3e-158	60.04	Dihydrofolate synthetase OS=Arabidopsis thaliana OX=3702 GN=DHFS PE=1 SV=1	[more]
Q05865	1.9e-40	32.61	Dihydrofolate synthase/folylpolyglutamate synthase OS=Bacillus subtilis (strain ...	[more]
P15925	9.8e-29	26.73	Folylpolyglutamate synthase OS=Lactobacillus casei OX=1582 GN=fpgS PE=1 SV=1	[more]
D4GW05	1.4e-27	28.86	Probable bifunctional folylpolyglutamate synthase/dihydropteroate synthase OS=Ha...	[more]
A6H751	1.6e-26	36.10	Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV...	[more]

BLAST of MS000468 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CJG1	2.1e-300	95.94	dihydrofolate synthetase isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110115...	[more]
A0A6J1E819	1.1e-269	85.34	dihydrofolate synthetase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11143073...	[more]
A0A1S3CNR7	2.2e-265	85.34	dihydrofolate synthetase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503019 PE=3...	[more]
A0A0A0L7A4	1.6e-263	84.63	Mur_ligase_M domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G35454...	[more]
A0A6J1J6Z7	9.8e-258	83.39	dihydrofolate synthetase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483133 ...	[more]

BLAST of MS000468 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41480.1	9.3e-160	60.04	Folylpolyglutamate synthetase family protein	[more]
AT3G55630.3	5.1e-25	42.59	DHFS-FPGS homolog D	[more]
AT3G55630.2	1.5e-24	42.59	DHFS-FPGS homolog D	[more]
AT5G05980.1	2.5e-24	41.61	DHFS-FPGS homolog B	[more]
AT5G05980.2	2.5e-24	41.61	DHFS-FPGS homolog B	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013221	Mur ligase, central	PFAM	PF08245	Mur_ligase_M	coord: 103..254 e-value: 3.6E-8 score: 33.6
IPR001645	Folylpolyglutamate synthetase	TIGRFAM	TIGR01499	TIGR01499	coord: 81..562 e-value: 1.7E-84 score: 282.4
IPR001645	Folylpolyglutamate synthetase	PANTHER	PTHR11136	FOLYLPOLYGLUTAMATE SYNTHASE-RELATED	coord: 46..564
IPR036565	Mur-like, catalytic domain superfamily	GENE3D	3.40.1190.10		coord: 40..368 e-value: 1.0E-84 score: 286.1
IPR036565	Mur-like, catalytic domain superfamily	SUPERFAMILY	53623	MurD-like peptide ligases, catalytic domain	coord: 78..368
IPR036615	Mur ligase, C-terminal domain superfamily	GENE3D	3.90.190.20		coord: 402..566 e-value: 1.3E-13 score: 53.4
IPR036615	Mur ligase, C-terminal domain superfamily	SUPERFAMILY	53244	MurD-like peptide ligases, peptide-binding domain	coord: 409..564
None	No IPR available	PIRSR	PIRSR038895-1	PIRSR038895-1	coord: 42..562 e-value: 4.5E-51 score: 171.7
None	No IPR available	PANTHER	PTHR11136:SF0	DIHYDROFOLATE SYNTHETASE-RELATED	coord: 46..564
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01011	FOLYLPOLYGLU_SYNT_1	coord: 101..124
IPR018109	Folylpolyglutamate synthetase, conserved site	PROSITE	PS01012	FOLYLPOLYGLU_SYNT_2	coord: 201..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000468.1	MS000468.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009058	biosynthetic process
biological_process	GO:0009396	folic acid-containing compound biosynthetic process
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0004326	tetrahydrofolylpolyglutamate synthase activity