MS000462 (gene) Bitter gourd (TR) v1

Overview
NameMS000462
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncation/H(+) antiporter 3-like
Locationscaffold44: 1874555 .. 1885562 (-)
RNA-Seq ExpressionMS000462
SyntenyMS000462
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGTTCAATTCAGACTTGAGGATCACAAACTCAACCGTAGATATTATTCCCACTACCATTTGTCTGTTAATGCCACCTCGGGTTGGCTCTAATGGCATTTGGGGAAACTCCTCTACCTCTGAATGGTGGCTCAACAATTCATTGCCTCTCTTGGAGTTGCAGCTCTTCTTCTTCTTTCTCCTAATGTCTATTATTCATCTTCTCTTCAAGCCCTTTGGGGTCCCCAAGATTTCCTCTCAGATTATTGTAAGCACACCAAAATCTTCTTCTTTTTGGGTTGATATTAATTGTTTAGAAATGGGTTATTTCTAGTAATCGCATACACTTTGTAAACTAGAAGGCAAGTTTTGATTTTTCACTGTGTTTATATCTTTTCCTGTGATATTACTATTCGTGTGAGTGAATAAGATGAGCAGAACAGAGACTAATTTTTTATATTTTGGCAGGCTGGTTTGGTAGTTGGTTGTTCATGGGGCCCATTGGACGACACAAAATTCATACTTTTTAGGATTAACAGTGAAGAGATCCTGGGGCTGTTATCCTACTTCGGCTACATGCTATTTTTGTTCATAACAGCAGCGAAGATGGACGTGAGAATGATCTTGAAGACAGGAAGAAAAGCCTGGATAATCGGAATCCCTTCAACGTTAATTCCTATAATTTGTGGTTTTTGGGTTGGATCTTATTTCCTGGATGGCTTAAGTCCCGCAGAGAAAAATAGAGTCCCTGTAATTGTTAGCATCCAGAGTTTTATCTCATTCCCAGTGATTGCTTCTCTTCTGAGCGAGCTGAAGATGGTGAGCACAGAACTTGGGCGTTTGGGGCTGTCCTCAGCGTTGATTGCTGACCTATTAGGCCAATTTGTTATGGCGACTGCAAACCAAATCAGAATCTCCAGAAGCAACATTACACTTGGCTATTTTAACAAGGCAGGAATTATGGTTCAATTTTGTCTCATAATTTTTGTGCTGAGGCCATCAGTGCTTTGGATTTTGAAACAGACCCCTGAGGGGAAGCCAGTGAGCCGTGCCACTACTCACGGTGTCTTTATTTTGGTTCTACTTTCTGCTGTTTCTTCTGCTCTTTTGGGGCAGCCAGCAGTTTTTGGGCCTTATCTTTTGGGGCTGGCCATCCCAGATGGAGCTTCTGGATTGGGGTTCTCCCTGGTGGAGAAAATGGAGTGCTTTGTTTCAGACTTCTTTATGCCATTGTTTGTCATAACATGTTCTCTACAGGCCGATGTTTCCAAGATGTTCCTTGTCTTTGCAGCTGCTGCTTTTGCAAGAATTAACATAATTATTACACTTGTCACTTATGGAGTCAAACTGGTGTGTTGCTTCCTTTGTTCTTTGTATTGCAAGTTGCCCTTGAGAGATTGCTTCGTCCTGTCCCTCATTCTTAGCTGCAAAGGTGTGGTTGAGTTGGGCTTCTACTTAGTCACCCATCAATACAAGGTCCGATTTGTTTCATTTTGGTCTCTTAACGTTTTAAAAAGCAACCATTTTGGTTGGTTTATGACTTTCAAAATGTAAAATGCTTGTTTTAGTGATCAGATTAACCATCTTTTTCCTTGTTTCTTTGATCCCATTTCCAGATTATCAACCACGGGATTCTGGCATGGTTCATACTGTTCTTATTAACAATTGCAACCTTTGTGCCACTGACAGTGAAATGGCTCACAGACCCCAAAAGGACACACAGTAGCCATCAGAACAGAAACATCATGCATTTAAGCCCCAACTCAGAATTGCGGATCCTTCCCTGCATACACAAAAACGAACACATCTATGGCCTCATCCATCTCCTCAATCTCTCATGTCCAACGCCGCAAAACCCGCTCGCCGTTTACGCCCTTCACCTCATCGAGCTCGTCGGCCGCACCACCCCCATCTTCATTTCCCATAGACAGGAAAACAAGGCCATTGCCGACCAACCCTACTCAGAAAATGTCACTCTCTCCTTTGACCACTTTGAGAAGGACAACTCTGCAGGCTCTGTCTATGCTGAATGCTTCACAGTAATCACACCCCACAAGTTCATGACCAATGACATCTGCAAATTGGCGCTTGACAAGGTCACGTCTCTTATAATTCTGCCCTTCCACAGGACATGGACGGCGGATGGCCTTATAGACCAAGAGGACAACATGTTGAGGAATTTGAACTGCAGTGTGCTCCAGAAGGCGGATTGTTCAGTGGCAATTTTTGCTGACAAAGGGCATTTAGGGAGCATTGCTTCTATGGCTTCTTCTGGCACACAATTTAGATATTCAGTTTGTTTGATATATTTGGGAGGGAGTGATGACAGGGAGGCAATTTCTTTTGCCAAACGCATGGCAAAGGAGCCCACAATTGAGCTCACTGTGCTGAAACTTGGGTCTCCTATGGAAGATGGGTCGAGTAGCAAATGGGAGAACATGCTTGATTCTGAGGTTCTAAAGGATTTCAAAATGAACTGTTTGGGGAATGGGGGAGTGAGGTATTTTGAGGAAGTGTGTGATGATGGGCCTCAAACAGCACTGACACTTAGAAAAATTGTGAATGAGTTTGATCTTATGATAGTAGGGAGAAGAGATGGGTTGGAATCTCCTCAGACTGCTGGGCTTAGTGAGTGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCACTTGATAACAACACAAGAACTTCTGTGTTGGTAATACAACAGCAGAAGTAATTAATTAATTAATTCACTAGACATATCAAGATGTGCATATACTCTAATTATGTATCTTTTTGCAGAGTATATGTAGAACAATACTCTTGTTTATCATCAACTTTAATTTTTTGCAGTTTGTTTGTGTCTGATAAATGAAAATGAAGGGAATTTTCAGCTTCTTAGATTGGTGCATTTATTCATTCATTTCTTGTAAGCAAAGACTAATAATTATTTAGTCAAATTAATATGTGTTGAGTTGGTAAAGAAACAAAGTCCCACGTTGACTAGGGAAGAACATGATCCATGGTAGATGAGGACAATTATCTTCATTGATATGATACTTTTTGGGTGAAATCAAAAGTAAAGCCGTGAGAGTTATGTCCAAAGCGAACAATGTGATACCATGGTGAAGATACTTGGGGTAAAAGGGGGACCGTTGTCCTTAACAAGCACCAATATTTTGACACTATCTAAACTACTTGCAACAGTTTAATCAACACAAACAGAGAATACGAAATATGAAAGAAAAGCGAACGTCAGAGTTTATTCTAGTTCACCTCAAGTTTGGGATTTCATCTAGTCTTCTGCAATATGCAGGTTCTAATAACAAATCTATCTTAGAGATAGTAACATATCCTCCAATATCAATTCAAGAAGAAGTGTGACAATCGTGCATAGGGGTGTATGAATAAATGGACACTGTGCTATAGCAATGAGAGAGGTAATCCTAAGACTATTGCAAGTATATAGTTATGAACCAACATGAGTTTAGATCAATTGGTTAAAGCATATATCTCAACTTAAATATCGTAGGTTCGAATCCTCACCCTACAACTCGACAAGTTGGTAAGAACGGTTCATAAATTTGTTAGACGCCCTGTTTAAAATTTAAAACTTAATAATAATTTGATATAATTATCTCAAAAGGTTTATGGGTGTTAAATAATATTTCTCAAGTATGGGAAAGAAGCCTCTAGAAACTACTAGGTTAAGTGGTTTGCCAAATCTTGCTCAAATAGCCTCTTTAGAGATAGAAATCTCTAGAAGCCACTAGGATAAGTAAGCTCGTTCAAAGAGTTTTATTGGAGAATTGAAGAAACTAGAAGCTATTAGAATGCAATATGTGGCCACTAGATTATCACCTATCCAAGGTTAGTTTGTGGCATAATTATAGATAGAATTTTGCTTAATGAGCAAGTATGAATGGGCTATAAATAGGAGCGGACCCATACTTGGCAAACTATCCCAAAAATTCTCAAATTCCAAGTGAGCTAAAGTGTGAGTGTGAGGATTGGTGAGATAAAGATAGAGAGAGTTTCTTCTTACGAGAGATTTTGTTCTCCATAAGAAAGAATTTAACTCATACTTTATTGAGTGAAATTCTTCTTCCTTATTCGTGGTTTTTACTCTAATTCATTTAGGAGTTTTCCACGTAAAATCACTGAATCATTTTCTTCTTCTTCCCCTTTACCTCTTCTCAATCCATTTTAACTGCGTCTTACTCTATTCGATTCCAATCATTTTGGCAACCATGGGTACAATTCAATGACCCTAATTGGTGGCTCAAGGGTTCTTTGCCCCTCTTGTAAGAATTTTTATATTGGTGCGTTAAATTACAAAGTTAATTTTGTTCGGTCTAACAGATGCACGTACCTTAACGTTTCAAATAGGTTCTTAAAACTCTCAAGATTGTATCAATCCAATCCCTATCCTTTTTAAAAAAGGTTTTCCAAACTTTCAATTTTGTGTCTAAATAATAGATCTCTTCGTTAGTTTAATTACTAACGTTATTATTTAAGAATTAAGCGTATGATTTCCAGAACCAAACGTATAATTTACTGTATAATCTACTATTTTTTCAAAGTATAAGAATGAGAGATTCAATTTAGAGAATTCAAGAATTAAACTTATAATTAAACTTATAAAAATTATAGGATTCTTAGCAGATTTTGGGTACACCTTGTATTATTCCTGTCCACAGCCAAAATGGACGCGTCAATGACAATAAGAGCTGGAAAGAAAAAGCACTGACGATCGAAATTCCAGCGTTTTCAGTCCCTCTAATCACTGCAACTTTGGTTCAAAGGCTTTTAGATGATGGCATACACCAGAAACAGGCTTAACCCTACTACCCATCTTGATTTCGTATGACTTTGTTTCCCGCGGTGGGCCGTTTTGGGGCTTTCCACGGCCTTGGTTCAATGACTTGCTTCTTAAGGATAAATAAACTATCCTTCAACCCAAAAGTTTAAATTAATGGGTTAGTTCTACAAGTGAAAATCATTATAAATGAGGAAAAAATAACAACTTAGAAGTTCGAACTCTGATACCATGTTAAAGATGAATAAACTCAACCCAAAAGTTTAACTAATGGATTGGGTAAATTTAATTCATATATATGGATTCTCAACATTGCTCGACCTCTTCGTGAGGGTCTCAACAAACCCAGAAGGGATGCCAGTGAAGGGTAGCTACATTCAAGCTATCGTTTTTTTTTTTTTTTTTTACTCTTCTTACCACTGTGTTGTCCAATTTTATTGGCTAGGTCTCCTTGATGGGGCCTTATATCTTGGGTTTGGCTGTTTCTGACGAGTCTGGGTAACGTTTCTATTTCTTCATTTTTAATAAAGAAAAATTAATTACATTGCTCCAACAACCATGAATTCAAGTATGATATAAAACAATAATTCTATTTATGTTTTGGAACAAATTAAAAATGAGAACAAAGAAATAAAAAACTTGTTTTTGTTGTTTTTAAAATTTCTATACAATTTTGAGAATGTTCCCAAAAAGGGAGGAACAAGAAACATAATTAGTTGAAAAATGCTTAGATTTTTTAAGAAAATTAGAAACAAAAAAGGTGAACGTTATCAAACGAGAGCCTAAATTTATTGTGGAATTGATTATCTACACTATATATGGCCAGAGATTACAATTGATTTCATCTCTCTCCTCCATCTTCCATCTTCATTATATATTGGGTTGAGAAAACAGGAGTTTCTAATTTGTTTGATTTAGGGTTCAATATATGTTTTTAGTTTGTTAGTAATGTTTGAAGGGTTTTTTGGATTTAGTTTCCCTCTATATTTTGTAATTAGCTTTTGATTTAGTTTCTTAGGCATGTCTTAACAATTTTTGGGAGTTGAAGTCAAACCGAGCATAGTTCAATTGATTAAAGTACCTACCTCTAATCAAAAGATCATGAGTTCGAATCTTCACCCTTACATGTTATACTAAAAAAGAATTTGAGAGTTGAAATTTTTGTTACTATTTGGTGCTCTAATTCTTCTTTTAGATAACTCGAGTTTATCACTTATATTTAACATTTATTAATGAAAATTATATAATTAGAAAAAAAATGTTACAACTAATTTTTTTTAAACTATTTGAAACGATCGAAACATATATCTAGTATTTTGGTTTTAACGGTTCTTTTTTTGTTTTGTTTTTCTAACTTGGATTCAACAACTACAGATATCAACGATGGGCTTCTTGCATGCTTCGTTCTTGCCAATTTTTTTTGTTACAACATTGGTGCCAATTGCACTGAAGTGTATCTATGATCCTTCGACGAAATATGCTGCATCATGCATTTGAATCCCTCCGACGACGAGCTTCGACTGCATTCACCGGAATGAAATATCAACGGCATCATTCATCTTCTCAATCTCTCATGTCCCACGGCGGAAAACACGCTCGCCATTCATGTATTCCATCTCATTGAGCTAGCTCTCCCGTCGATCGGACCGCCCCAATCTTCATTTCTCACAAATATTTTCACAAGGCCCTCAATGATCGATCATAATCTAAACATATTTATTCGTTCTTTTGATCGGTCTGAGAGAGACAACGATGATGGAACAGTTTACGTCGAATGCTTCACCGCATGCAATCTCGTCAAACCGGTTAATGCACGACGATGTATGCATGCTCTTGCTCAACAAGATCACATCCCTCATTTTCCATCGAACCTGGACAGTGGATGGTTTTGTCTACCACGACGACAACATGATCAGGACATTGAACTGCAGCGTGCTCGGAATGGCTCCATGCTCGATTGGCATCTTCACCAACCAAGGACGTTTAGGGAGCATTTCCACAACAGCAACACCGAGAATGAAATGTAAATACTCGGTCACTCGTAGAGATTGATAAATGATTTCATTTATTCTAGGGTTGGTACATGAGAGATGAGATAATTATGAGAATTTTGCACCGAATTAGTATGACAAAGAACTAACCATTGCACCTAGGGATGGTACAGAAAGACGAAGGATCAGTAGCTGAACTTGAGTTTTGCGAAGAGTTTGAGTGAATCCATTCTCAATGTGAACGTATAAAATCAGGAGGGTCGGAATATTTACCTTGAAATAGACTCTATTGGAAGCGAAGAAGCTGTTGAACAAGGAAGAGGCCTACATCTCGGAAATTTTCTGGGTCGCGGACCAAATTCGATCAGTACTTTTGAAGAAAAATCAGTGGTCTAATAATCAATTTTATTAATTTAAAAAGTGTGACAATCGTATACGAGGGCTCATGAATCAACTGCCAATATATAGTCTAAATACTCAAAAGAAATTATAGTTTAACTATTATCTATACCAACAAAATTCTGCTTTCTTTTTAGTCTATTAATAAAAACTCCAATATAGAAGGATAGAAACTTTATATTTTTTGAAGTTTATAAATATATTAGATGTCATCATCGAAAATTAAAATTATAGTAGTTGTTCTTTTATTACGGAATTATATATAGACGAGGATTCAAACCCACCATCTTTTGATTCAAAATGAAGTTTTTTTTAGTACATACTCCGGATATATACAGTTAAGCTGACAATGAGATACTCAAGGACGACCATTACAAGACAAAGTGATGCACAAACTCCGTAGATTTCGATGAAATATAGGGAAAAACTAACTCCCAAAGGAACAAACACAACCCTATTAATGGTTAGAGTTGCATTTCTTCCTCAAATCTCCTAAATATAATCAGCCGAATAATCATAAACTCAACAAAATCTAATGTAAATTTATAGTCAAAATCGTTTCTAGACTTTAAATAAACTATCATGTAATGATGAAAACAGCTACTTATTTTTTTGTTTTTCCTCTCGGTTTGACTTATATCTAACCGACCCATATAAAAAAGGAGGTGAATTTTGAAACGAAAACCAACCATTTTTGTGTGGAAATCATCAAATTTCTTCTTCTTCCTTGTGGGCTGTTGAGAATTTTTTGTGTGGAGAAGGGAGAAGATGCAGTCGAATTATTCAAGTTCGAAGGAGGAGTCCCATTTATCATATTATTCAGAATCAAATTCCACGAGTGTGATTTGCCTAGATGTTCCATCTCATATAATCTCAAAAGGTTTATGGGTCCAATTCAATGACCCTAATTGGTGGCTCAAGGGTTCGTTGCCCCTCTTGGAGTTTCAATTGACTGTTTTGTGCTTCTCATTGGTTATTACCCATCTTATTCTCAAGCATTTTGGGGTCTCCAAAGTTTCCTCTCAAATTCTTGTAAGTACACACAAATCCTCCTACACCCTCTTCTCAATCATTTGTAACATTTGGTTGTTAGTAAACTTGTAAGAATTTTTATGTCAGTGTTAAATTACAAAGTTTGGATCTTTTTGTTAGTTTAATTCATGCTCTCATGGCAGGCTAACGTGTAAAAAATGTTGATTTCAAATAAGTTCGATCTCCCATATCACAAAACTGTTCGGCCAACCCGCATGATTCCTCTAGCTAGTTTCAAATAACAATATAATCGCCTTCTTTTTCTTGCATTAGGGGAAAAGGGTTTCCTCCTATTCCTAACTTTTACACAAAATGGCTATATTTGATCAACAACTTCTAACTGATCATGTGACTGAAAAATGAATCAGATTGGGCTGATATTTGGGTGCTCGTGGAGGCAGTTCGACAAGGAGACAATCAATCTTTTCTACGAACGCAACCAAGACATTCTAGGATTGTTGGCAGAATTCGGGTACACCTTGTACTTGTTCCTGTCCGGAGTCAAAATAGACCTGTCAATGACAATCAGAGCCGGAAAAAATGCACTCATCATCGGAATTCTAGCCTTATTAGTCCCTCTGTTCGCTGCAAATTTTGTTCGAAGCCTTTTAGTTGATGGCAGTGGCATATCTGAAGCAGACTTATCCACACTCCCCATCTTGATTTCCTTCCACAGTATGTCTTCATTTCCCGTAATCGCCTCTCTCTTGAGCGACCTTGAGATTGTGAACTCGGAATTGGGGCGTTTGGGGCTCTCCTCCGCCTTGTTCAGCGACTTGCTCAGCCTCTTCATGATGGTCACCGCCAGACAGGCCAAACGATTCAAAGATGTGCCTTCAATAGCTTCTCTCCAACTCGGGGCTCTAATTTTGCTCTTCCTTCTGATTCTATTTGTGTTCAGGCCAGCCATGCTTTGGATCATCAGACAAACCCCGGAAGGGATGCCGGTGAAGGGTAGCTACATTCAAGCTGTCATTTTTTTGGTTCTCTTCACCACTGTGTTGTTCAATTTCACTGGCCAGATTTCCATAATGGGGCCTTATGTCTTTGGTTTGGCTGTTCCTGATGGGGCCCCTTTGGCCTCCACTCTTGTGAACAATATTGAGTGCCTTGTTTCGGATGTGTTTATGCCCATTTTGGTGATTACCTGTGCTTTGAAAGCGGATCTGTCGCAGATTTCTTCCTCGTTTGATGCTGCTTTCACAAAGTTGAACATAGTTCTCATTCTTGTCACTTTTGTCGTCAAGATTGTGTCTTGCTTTCTGACTTCCAGGCATTGTAGGCTGCCCTTCAGGGATTCCTTGGCACTTTCTCTCATCATGAGCAATAAAGGTGCTGTGGAATTGGTTCTCTACACGATGGCCAGAGATTACAATGTATGTATCCTTGATTTCATCTCTCTCCTCCACCTTCACATATTGGGTAGAGAAAGAATGGTTCTAGAATTTGTTCCTGTCATGTCATATAAGTCAATCACCCATATTTTAACATTTACTTCACTAATGGAAATGATATAACTAGAAAAAATGTTACAACTAGGTTCTTTTAAACGATAGGACTGAATTGAAAACACATTAGAGATTGAAAGTATATTTAGTGGTTTGGTTTTAATGGGTTGTGTGTTTTTCTTTTCTTTTTCTAATGTGGATTGGAACAACTACAGGCTATCAATGATGGGCTTCTTGGATGGTGCTTCCTTTCCATAGTTTTTGTTGCAACATTGGTGCCAATTTTGGTGAAGTGTCTCTATGATCCTTCGATGAAATATGCTGGCTATCAGAATAGGAACATCATGCATTTGAATCCTGCCTCCGACAAGCTTCGCCTGCTTGCCTGCATTCACCAAAATGAAAATATCAACAGCATTATTCATCTTCTCAATCTCTCATGTCCCACGGCAGAAAACCCGCTTGCTATTCATATATTACATCTCATCGAGCTCTCCGGTCGGACCGCCCCCGTCTTCATTTCTCACAAGTATTTTCACAACAAGGCCCTCAACGATCGATCCTACTCTCAGCACATTATTCATTCTTTTGATCAGTTTGAGAGAGACAGCGATGGCACGGTTTGCGTCGAATGCTTCACCGCAATCTCGCCAAACCGGTTAATGCACAACGACATATGCACCCTTGCGCTCGACAAGATTACATCCCTCATTATAATGCCATTCCATCGAACATGGACAGTGGATGGTTTTGCCTACCACGACGACAACATGGTCAGGACATTGAACTGCAGCGTGCTCGGAAGGGCTCCATGCTCGATCGGCATCTTCGCCGACCGGGGACGTTTGGGGAGCATTGCTGCAACGGCAATACCGAGAAGTAGATACTCAGTCTGCGTGCTCTTCTTGGGAGGGAAGGACGATAGGGAGGCAATATCGTATGCGAAACGCATGGCAAAGGACTCAAGAGTGGAGCTGACAATGCTCCGACTCCGGGGGCCGCCAGAGGAACATGGACAAAAGACAGACAATTGGGAAGATATACTTGATGCTGAAGTTCTAAGAGATTTTAGAAGCAAGTGTTTGGGAGATGGTAGAGTGGTGTACAAGGAGGACGTGTGCAGAGATGGGCAACAAACGGCATTTATTATTAGAAAAATAGTAAATTTGTTTGATCTTATGATAGTAGGGAGAAGAAATGGCTTAAAATCGTCTCAAACTGATGGTCTCAATGAATGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCAACAGATATCAACAGTAGAGCTTCTTTGTTGGTAATACAACAACAACAGATCGTGATTCAA

mRNA sequence

ATGACGTTCAATTCAGACTTGAGGATCACAAACTCAACCGTAGATATTATTCCCACTACCATTTGTCTGTTAATGCCACCTCGGGTTGGCTCTAATGGCATTTGGGGAAACTCCTCTACCTCTGAATGGTGGCTCAACAATTCATTGCCTCTCTTGGAGTTGCAGCTCTTCTTCTTCTTTCTCCTAATGTCTATTATTCATCTTCTCTTCAAGCCCTTTGGGGTCCCCAAGATTTCCTCTCAGATTATTGCTGGTTTGGTAGTTGGTTGTTCATGGGGCCCATTGGACGACACAAAATTCATACTTTTTAGGATTAACAGTGAAGAGATCCTGGGGCTGTTATCCTACTTCGGCTACATGCTATTTTTGTTCATAACAGCAGCGAAGATGGACGTGAGAATGATCTTGAAGACAGGAAGAAAAGCCTGGATAATCGGAATCCCTTCAACGTTAATTCCTATAATTTGTGGTTTTTGGGTTGGATCTTATTTCCTGGATGGCTTAAGTCCCGCAGAGAAAAATAGAGTCCCTGTAATTGTTAGCATCCAGAGTTTTATCTCATTCCCAGTGATTGCTTCTCTTCTGAGCGAGCTGAAGATGGTGAGCACAGAACTTGGGCGTTTGGGGCTGTCCTCAGCGTTGATTGCTGACCTATTAGGCCAATTTGTTATGGCGACTGCAAACCAAATCAGAATCTCCAGAAGCAACATTACACTTGGCTATTTTAACAAGGCAGGAATTATGGTTCAATTTTGTCTCATAATTTTTGTGCTGAGGCCATCAGTGCTTTGGATTTTGAAACAGACCCCTGAGGGGAAGCCAGTGAGCCGTGCCACTACTCACGGTGTCTTTATTTTGGTTCTACTTTCTGCTGTTTCTTCTGCTCTTTTGGGGCAGCCAGCAGTTTTTGGGCCTTATCTTTTGGGGCTGGCCATCCCAGATGGAGCTTCTGGATTGGGGTTCTCCCTGGTGGAGAAAATGGAGTGCTTTGTTTCAGACTTCTTTATGCCATTGTTTGTCATAACATGTTCTCTACAGGCCGATGTTTCCAAGATGTTCCTTGTCTTTGCAGCTGCTGCTTTTGCAAGAATTAACATAATTATTACACTTGTCACTTATGGAGTCAAACTGGTGTGTTGCTTCCTTTGTTCTTTGTATTGCAAGTTGCCCTTGAGAGATTGCTTCGTCCTGTCCCTCATTCTTAGCTGCAAAGGTGTGGTTGAGTTGGGCTTCTACTTAGTCACCCATCAATACAAGATTATCAACCACGGGATTCTGGCATGGTTCATACTGTTCTTATTAACAATTGCAACCTTTGTGCCACTGACAGTGAAATGGCTCACAGACCCCAAAAGGACACACAGTAGCCATCAGAACAGAAACATCATGCATTTAAGCCCCAACTCAGAATTGCGGATCCTTCCCTGCATACACAAAAACGAACACATCTATGGCCTCATCCATCTCCTCAATCTCTCATGTCCAACGCCGCAAAACCCGCTCGCCGTTTACGCCCTTCACCTCATCGAGCTCGTCGGCCGCACCACCCCCATCTTCATTTCCCATAGACAGGAAAACAAGGCCATTGCCGACCAACCCTACTCAGAAAATGTCACTCTCTCCTTTGACCACTTTGAGAAGGACAACTCTGCAGGCTCTGTCTATGCTGAATGCTTCACAGTAATCACACCCCACAAGTTCATGACCAATGACATCTGCAAATTGGCGCTTGACAAGGTCACGTCTCTTATAATTCTGCCCTTCCACAGGACATGGACGGCGGATGGCCTTATAGACCAAGAGGACAACATGTTGAGGAATTTGAACTGCAGTGTGCTCCAGAAGGCGGATTGTTCAGTGGCAATTTTTGCTGACAAAGGGCATTTAGGGAGCATTGCTTCTATGGCTTCTTCTGGCACACAATTTAGATATTCAGTTTGTTTGATATATTTGGGAGGGAGTGATGACAGGGAGGCAATTTCTTTTGCCAAACGCATGGCAAAGGAGCCCACAATTGAGCTCACTGTGCTGAAACTTGGGTCTCCTATGGAAGATGGGTCGAGTAGCAAATGGGAGAACATGCTTGATTCTGAGGTTCTAAAGGATTTCAAAATGAACTGTTTGGGGAATGGGGGAGTGAGGTATTTTGAGGAAGTGTGTGATGATGGGCCTCAAACAGCACTGACACTTAGAAAAATTGTGAATGAGTTTGATCTTATGATAGTAGGGAGAAGAGATGGGTTGGAATCTCCTCAGACTGCTGGGCTTAGTGAGTGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCACTTGATAACAACACAAGAACTTCTGTGTTGATTGGGCTGATATTTGGGTGCTCGTGGAGGCAGTTCGACAAGGAGACAATCAATCTTTTCTACGAACGCAACCAAGACATTCTAGGATTGTTGGCAGAATTCGGGTACACCTTGTACTTGTTCCTGTCCGGAGTCAAAATAGACCTGTCAATGACAATCAGAGCCGGAAAAAATGCACTCATCATCGGAATTCTAGCCTTATTAGTCCCTCTGTTCGCTGCAAATTTTGTTCGAAGCCTTTTAGTTGATGGCAGTGGCATATCTGAAGCAGACTTATCCACACTCCCCATCTTGATTTCCTTCCACAGTATGTCTTCATTTCCCGTAATCGCCTCTCTCTTGAGCGACCTTGAGATTGTGAACTCGGAATTGGGGCGTTTGGGGCTCTCCTCCGCCTTGTTCAGCGACTTGCTCAGCCTCTTCATGATGGTCACCGCCAGACAGGCCAAACGATTCAAAGATGTGCCTTCAATAGCTTCTCTCCAACTCGGGGCTCTAATTTTGCTCTTCCTTCTGATTCTATTTGTGTTCAGGCCAGCCATGCTTTGGATCATCAGACAAACCCCGGAAGGGATGCCGGTGAAGGGTAGCTACATTCAAGCTGTCATTTTTTTGGTTCTCTTCACCACTGTGTTGTTCAATTTCACTGGCCAGATTTCCATAATGGGGCCTTATGTCTTTGGTTTGGCTGTTCCTGATGGGGCCCCTTTGGCCTCCACTCTTGTGAACAATATTGAGTGCCTTGTTTCGGATGTGTTTATGCCCATTTTGGTGATTACCTGTGCTTTGAAAGCGGATCTGTCGCAGATTTCTTCCTCGTTTGATGCTGCTTTCACAAAGTTGAACATAGTTCTCATTCTTGTCACTTTTGTCGTCAAGATTGTGTCTTGCTTTCTGACTTCCAGGCATTGTAGGCTGCCCTTCAGGGATTCCTTGGCACTTTCTCTCATCATGAGCAATAAAGGTGCTGTGGAATTGGTTCTCTACACGATGGCCAGAGATTACAATGCTATCAATGATGGGCTTCTTGGATGGTGCTTCCTTTCCATAGTTTTTGTTGCAACATTGGTGCCAATTTTGGTGAAGTGTCTCTATGATCCTTCGATGAAATATGCTGGCTATCAGAATAGGAACATCATGCATTTGAATCCTGCCTCCGACAAGCTTCGCCTGCTTGCCTGCATTCACCAAAATGAAAATATCAACAGCATTATTCATCTTCTCAATCTCTCATGTCCCACGGCAGAAAACCCGCTTGCTATTCATATATTACATCTCATCGAGCTCTCCGGTCGGACCGCCCCCGTCTTCATTTCTCACAAGTATTTTCACAACAAGGCCCTCAACGATCGATCCTACTCTCAGCACATTATTCATTCTTTTGATCAGTTTGAGAGAGACAGCGATGGCACGGTTTGCGTCGAATGCTTCACCGCAATCTCGCCAAACCGGTTAATGCACAACGACATATGCACCCTTGCGCTCGACAAGATTACATCCCTCATTATAATGCCATTCCATCGAACATGGACAGTGGATGGTTTTGCCTACCACGACGACAACATGGTCAGGACATTGAACTGCAGCGTGCTCGGAAGGGCTCCATGCTCGATCGGCATCTTCGCCGACCGGGGACGTTTGGGGAGCATTGCTGCAACGGCAATACCGAGAAGTAGATACTCAGTCTGCGTGCTCTTCTTGGGAGGGAAGGACGATAGGGAGGCAATATCGTATGCGAAACGCATGGCAAAGGACTCAAGAGTGGAGCTGACAATGCTCCGACTCCGGGGGCCGCCAGAGGAACATGGACAAAAGACAGACAATTGGGAAGATATACTTGATGCTGAAGTTCTAAGAGATTTTAGAAGCAAGTGTTTGGGAGATGGTAGAGTGGTGTACAAGGAGGACGTGTGCAGAGATGGGCAACAAACGGCATTTATTATTAGAAAAATAGTAAATTTGTTTGATCTTATGATAGTAGGGAGAAGAAATGGCTTAAAATCGTCTCAAACTGATGGTCTCAATGAATGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCAACAGATATCAACAGTAGAGCTTCTTTGTTGGTAATACAACAACAACAGATCGTGATTCAA

Coding sequence (CDS)

ATGACGTTCAATTCAGACTTGAGGATCACAAACTCAACCGTAGATATTATTCCCACTACCATTTGTCTGTTAATGCCACCTCGGGTTGGCTCTAATGGCATTTGGGGAAACTCCTCTACCTCTGAATGGTGGCTCAACAATTCATTGCCTCTCTTGGAGTTGCAGCTCTTCTTCTTCTTTCTCCTAATGTCTATTATTCATCTTCTCTTCAAGCCCTTTGGGGTCCCCAAGATTTCCTCTCAGATTATTGCTGGTTTGGTAGTTGGTTGTTCATGGGGCCCATTGGACGACACAAAATTCATACTTTTTAGGATTAACAGTGAAGAGATCCTGGGGCTGTTATCCTACTTCGGCTACATGCTATTTTTGTTCATAACAGCAGCGAAGATGGACGTGAGAATGATCTTGAAGACAGGAAGAAAAGCCTGGATAATCGGAATCCCTTCAACGTTAATTCCTATAATTTGTGGTTTTTGGGTTGGATCTTATTTCCTGGATGGCTTAAGTCCCGCAGAGAAAAATAGAGTCCCTGTAATTGTTAGCATCCAGAGTTTTATCTCATTCCCAGTGATTGCTTCTCTTCTGAGCGAGCTGAAGATGGTGAGCACAGAACTTGGGCGTTTGGGGCTGTCCTCAGCGTTGATTGCTGACCTATTAGGCCAATTTGTTATGGCGACTGCAAACCAAATCAGAATCTCCAGAAGCAACATTACACTTGGCTATTTTAACAAGGCAGGAATTATGGTTCAATTTTGTCTCATAATTTTTGTGCTGAGGCCATCAGTGCTTTGGATTTTGAAACAGACCCCTGAGGGGAAGCCAGTGAGCCGTGCCACTACTCACGGTGTCTTTATTTTGGTTCTACTTTCTGCTGTTTCTTCTGCTCTTTTGGGGCAGCCAGCAGTTTTTGGGCCTTATCTTTTGGGGCTGGCCATCCCAGATGGAGCTTCTGGATTGGGGTTCTCCCTGGTGGAGAAAATGGAGTGCTTTGTTTCAGACTTCTTTATGCCATTGTTTGTCATAACATGTTCTCTACAGGCCGATGTTTCCAAGATGTTCCTTGTCTTTGCAGCTGCTGCTTTTGCAAGAATTAACATAATTATTACACTTGTCACTTATGGAGTCAAACTGGTGTGTTGCTTCCTTTGTTCTTTGTATTGCAAGTTGCCCTTGAGAGATTGCTTCGTCCTGTCCCTCATTCTTAGCTGCAAAGGTGTGGTTGAGTTGGGCTTCTACTTAGTCACCCATCAATACAAGATTATCAACCACGGGATTCTGGCATGGTTCATACTGTTCTTATTAACAATTGCAACCTTTGTGCCACTGACAGTGAAATGGCTCACAGACCCCAAAAGGACACACAGTAGCCATCAGAACAGAAACATCATGCATTTAAGCCCCAACTCAGAATTGCGGATCCTTCCCTGCATACACAAAAACGAACACATCTATGGCCTCATCCATCTCCTCAATCTCTCATGTCCAACGCCGCAAAACCCGCTCGCCGTTTACGCCCTTCACCTCATCGAGCTCGTCGGCCGCACCACCCCCATCTTCATTTCCCATAGACAGGAAAACAAGGCCATTGCCGACCAACCCTACTCAGAAAATGTCACTCTCTCCTTTGACCACTTTGAGAAGGACAACTCTGCAGGCTCTGTCTATGCTGAATGCTTCACAGTAATCACACCCCACAAGTTCATGACCAATGACATCTGCAAATTGGCGCTTGACAAGGTCACGTCTCTTATAATTCTGCCCTTCCACAGGACATGGACGGCGGATGGCCTTATAGACCAAGAGGACAACATGTTGAGGAATTTGAACTGCAGTGTGCTCCAGAAGGCGGATTGTTCAGTGGCAATTTTTGCTGACAAAGGGCATTTAGGGAGCATTGCTTCTATGGCTTCTTCTGGCACACAATTTAGATATTCAGTTTGTTTGATATATTTGGGAGGGAGTGATGACAGGGAGGCAATTTCTTTTGCCAAACGCATGGCAAAGGAGCCCACAATTGAGCTCACTGTGCTGAAACTTGGGTCTCCTATGGAAGATGGGTCGAGTAGCAAATGGGAGAACATGCTTGATTCTGAGGTTCTAAAGGATTTCAAAATGAACTGTTTGGGGAATGGGGGAGTGAGGTATTTTGAGGAAGTGTGTGATGATGGGCCTCAAACAGCACTGACACTTAGAAAAATTGTGAATGAGTTTGATCTTATGATAGTAGGGAGAAGAGATGGGTTGGAATCTCCTCAGACTGCTGGGCTTAGTGAGTGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCACTTGATAACAACACAAGAACTTCTGTGTTGATTGGGCTGATATTTGGGTGCTCGTGGAGGCAGTTCGACAAGGAGACAATCAATCTTTTCTACGAACGCAACCAAGACATTCTAGGATTGTTGGCAGAATTCGGGTACACCTTGTACTTGTTCCTGTCCGGAGTCAAAATAGACCTGTCAATGACAATCAGAGCCGGAAAAAATGCACTCATCATCGGAATTCTAGCCTTATTAGTCCCTCTGTTCGCTGCAAATTTTGTTCGAAGCCTTTTAGTTGATGGCAGTGGCATATCTGAAGCAGACTTATCCACACTCCCCATCTTGATTTCCTTCCACAGTATGTCTTCATTTCCCGTAATCGCCTCTCTCTTGAGCGACCTTGAGATTGTGAACTCGGAATTGGGGCGTTTGGGGCTCTCCTCCGCCTTGTTCAGCGACTTGCTCAGCCTCTTCATGATGGTCACCGCCAGACAGGCCAAACGATTCAAAGATGTGCCTTCAATAGCTTCTCTCCAACTCGGGGCTCTAATTTTGCTCTTCCTTCTGATTCTATTTGTGTTCAGGCCAGCCATGCTTTGGATCATCAGACAAACCCCGGAAGGGATGCCGGTGAAGGGTAGCTACATTCAAGCTGTCATTTTTTTGGTTCTCTTCACCACTGTGTTGTTCAATTTCACTGGCCAGATTTCCATAATGGGGCCTTATGTCTTTGGTTTGGCTGTTCCTGATGGGGCCCCTTTGGCCTCCACTCTTGTGAACAATATTGAGTGCCTTGTTTCGGATGTGTTTATGCCCATTTTGGTGATTACCTGTGCTTTGAAAGCGGATCTGTCGCAGATTTCTTCCTCGTTTGATGCTGCTTTCACAAAGTTGAACATAGTTCTCATTCTTGTCACTTTTGTCGTCAAGATTGTGTCTTGCTTTCTGACTTCCAGGCATTGTAGGCTGCCCTTCAGGGATTCCTTGGCACTTTCTCTCATCATGAGCAATAAAGGTGCTGTGGAATTGGTTCTCTACACGATGGCCAGAGATTACAATGCTATCAATGATGGGCTTCTTGGATGGTGCTTCCTTTCCATAGTTTTTGTTGCAACATTGGTGCCAATTTTGGTGAAGTGTCTCTATGATCCTTCGATGAAATATGCTGGCTATCAGAATAGGAACATCATGCATTTGAATCCTGCCTCCGACAAGCTTCGCCTGCTTGCCTGCATTCACCAAAATGAAAATATCAACAGCATTATTCATCTTCTCAATCTCTCATGTCCCACGGCAGAAAACCCGCTTGCTATTCATATATTACATCTCATCGAGCTCTCCGGTCGGACCGCCCCCGTCTTCATTTCTCACAAGTATTTTCACAACAAGGCCCTCAACGATCGATCCTACTCTCAGCACATTATTCATTCTTTTGATCAGTTTGAGAGAGACAGCGATGGCACGGTTTGCGTCGAATGCTTCACCGCAATCTCGCCAAACCGGTTAATGCACAACGACATATGCACCCTTGCGCTCGACAAGATTACATCCCTCATTATAATGCCATTCCATCGAACATGGACAGTGGATGGTTTTGCCTACCACGACGACAACATGGTCAGGACATTGAACTGCAGCGTGCTCGGAAGGGCTCCATGCTCGATCGGCATCTTCGCCGACCGGGGACGTTTGGGGAGCATTGCTGCAACGGCAATACCGAGAAGTAGATACTCAGTCTGCGTGCTCTTCTTGGGAGGGAAGGACGATAGGGAGGCAATATCGTATGCGAAACGCATGGCAAAGGACTCAAGAGTGGAGCTGACAATGCTCCGACTCCGGGGGCCGCCAGAGGAACATGGACAAAAGACAGACAATTGGGAAGATATACTTGATGCTGAAGTTCTAAGAGATTTTAGAAGCAAGTGTTTGGGAGATGGTAGAGTGGTGTACAAGGAGGACGTGTGCAGAGATGGGCAACAAACGGCATTTATTATTAGAAAAATAGTAAATTTGTTTGATCTTATGATAGTAGGGAGAAGAAATGGCTTAAAATCGTCTCAAACTGATGGTCTCAATGAATGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCAACAGATATCAACAGTAGAGCTTCTTTGTTGGTAATACAACAACAACAGATCGTGATTCAA

Protein sequence

MTFNSDLRITNSTVDIIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKISSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIVSIQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNEHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLIGLIFGCSWRQFDKETINLFYERNQDILGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDGSGISEADLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFMMVTARQAKRFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITCALKADLSQISSSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHLIELSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITSLIIMPFHRTWTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIAATAIPRSRYSVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPEEHGQKTDNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRRNGLKSSQTDGLNEWNEFPELGVLGDLIASTDINSRASLLVIQQQQIVIQ
Homology
BLAST of MS000462 vs. NCBI nr
Match: KAG7014725.1 (Cation/H(+) antiporter 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1604.0 bits (4152), Expect = 0.0e+00
Identity = 830/1546 (53.69%), Postives = 1105/1546 (71.47%), Query Frame = 0

Query: 16   IIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGV 75
            +I +T C+ MP  V S G+W + +  +WWL+ SLP LELQL  F L M+I HL+ K FG+
Sbjct: 57   MINSTTCVFMPIHVNSPGLWESYNLFDWWLSYSLPRLELQLALFCLSMTITHLVLKNFGI 116

Query: 76   PKISSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAAKMDVRMI 135
            PKISS+IIAGL+ GCSW   D  ++ LF + S++ L + +YFGYML+LF+ A KMDVRM+
Sbjct: 117  PKISSRIIAGLIFGCSWKQWDGERYKLFTLESQDTLSVFTYFGYMLYLFVLALKMDVRML 176

Query: 136  LKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIVSIQSFISFPVIASLL 195
             +T RKA+++ +P+ + P+ICG +V +  L  L    + +V ++VS+   IS PVI ++L
Sbjct: 177  SRTKRKAYLVALPAFVGPMICGHFVTTLLLPYLDRPSQKKVSLMVSLHCMISLPVIENVL 236

Query: 196  SELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLII 255
             EL M+S+++GRLGLS+AL+ D+L Q  +   N +R+ + +   G+F  AG  ++  LI 
Sbjct: 237  RELNMISSDIGRLGLSAALVGDMLSQLGLVAGNVVRVYQLSAVRGFFYLAGFFIEGFLIW 296

Query: 256  FVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDG 315
            FV +P+ LW++K+TP+GK V  +   GV  LVLLS+  S LL QPA+ GPYLLGL IPDG
Sbjct: 297  FVFKPATLWMIKRTPKGKLVGGSNIQGVMCLVLLSSAVSVLLSQPAILGPYLLGLIIPDG 356

Query: 316  ASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGV 375
             S L  S++E+++ FVS+ F+P+F+   +LQAD+S + LV     F + N+++  VT+ V
Sbjct: 357  -SPLVISMIERLDFFVSELFLPIFIAMSALQADLS-ILLVGFKTVFTQFNLVLAFVTFSV 416

Query: 376  KLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLT 435
            K++  FL SLY  LP+ D   L+ ++S KG+VEL F  +   Y +++ G+L W  L +L 
Sbjct: 417  KVISSFLGSLYSGLPVHDSLALAFLMSNKGIVELAFITILRGYSVVSDGLLIWLTLVILL 476

Query: 436  IATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNEHIYGLIHLLNLSCP 495
            +AT VP  VK+L +P   ++  QN+NI++L  NSELR+L C+H+ +  +GLI LLNLSCP
Sbjct: 477  VATLVPFVVKYLYNPSIKYAVSQNKNIVNLPKNSELRVLVCVHQEKDTHGLIQLLNLSCP 536

Query: 496  TPQNPLAVYALHLIELVGRTTPIFISHRQEN-----KAIADQPYSENVTLSFDHFEKDNS 555
            T QNPLA+  LH ++LVGR TP+FISH Q+N         D+ YSEN+ L F+ FE+D +
Sbjct: 537  TKQNPLAITVLHPVDLVGRITPVFISHNQDNYGNNPYGHQDESYSENIVLCFNRFERDQN 596

Query: 556  AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC 615
             G+V  ECFT ITPHKFM +++C+L L K +SLIILPFH+TWTADG +D++DN ++ LN 
Sbjct: 597  -GTVCVECFTTITPHKFMVSEVCRLELQKASSLIILPFHQTWTADGHMDRDDNTIKALNS 656

Query: 616  SVLQKADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEP 675
             V++ A CSV IFA++G+LG++ S         YSVC+I+LGGSDDREAIS+AKR+ K+P
Sbjct: 657  GVIESASCSVGIFANRGNLGNMMSENDC-----YSVCVIFLGGSDDREAISYAKRLTKDP 716

Query: 676  TIELTVLKL--GSPMEDG--SSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQT 735
             +ELT+L+L   S  ED   +   W+ MLDSE L+DFK NC G+G VRY E VC+DG  T
Sbjct: 717  RVELTLLRLFAHSTAEDEACNDQNWDKMLDSETLRDFKTNCFGDGRVRYIEAVCEDGTHT 776

Query: 736  ALTLRKIVNEFDLMIVGRRDGLE--SPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSV- 795
             + LRK+VN+FDLM+VGRR GLE  SPQT GL+EWN+FPELG+LGDLI SLD N R SV 
Sbjct: 777  VMGLRKMVNDFDLMVVGRRKGLEESSPQTCGLNEWNDFPELGILGDLIVSLDVNFRASVL 836

Query: 796  --------------------------------------------------LIGLIFGCSW 855
                                                              L+GL  GCS 
Sbjct: 837  GLWVKLNDPHWWLNNSLPLLELQLILFCFALVFAHHFLKRFGFSKISSQILMGLAIGCSR 896

Query: 856  RQFDKETINLFYERNQDILGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLV 915
             Q++     LF   +Q++L LL +FGY LYLFLS  KID++M+I+ GKN+L+IGI A++ 
Sbjct: 897  NQWEDAKSKLFGVGSQNVLVLLTDFGYALYLFLSAAKIDVTMSIKTGKNSLLIGIPAVMA 956

Query: 916  PLFAANFVRSLLVDGSGISEADLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGL 975
            PL    FVR+ L   + ++    + LPIL+ FH M+SFPV+ASL+S+L+IVNSELGRL L
Sbjct: 957  PLLIEGFVRNGLYGDTHLTRKQKAALPILMGFHGMTSFPVVASLVSELQIVNSELGRLSL 1016

Query: 976  SSALFSDLLSLFMMVTARQAKRFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTP 1035
            SSAL SD+  + +M+   QA RF + PS AS +L  L+LLFLL +FVFRPAM WII+QTP
Sbjct: 1017 SSALVSDIFGVLIMIAVGQANRFNNNPSKASAELSCLLLLFLLAVFVFRPAMRWIIKQTP 1076

Query: 1036 EGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLV 1095
            EG PV  SYIQ V+FL L +TVL +FTGQ  I+GP++FGLAVPDGAPLASTLV+ +E LV
Sbjct: 1077 EGAPVNSSYIQLVVFLALLSTVLASFTGQSPIIGPFIFGLAVPDGAPLASTLVDKLETLV 1136

Query: 1096 SDVFMPILVITCALKADLSQIS-SSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPF 1155
            SD+FMPIL+ TCAL+ D S+IS ++F   FTK+NI LI  +  +K V+  ++S++C+ PF
Sbjct: 1137 SDMFMPILITTCALRVDFSKISAATFHNGFTKMNISLICASVGIKFVTGVVSSKYCKFPF 1196

Query: 1156 RDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPS 1215
            +D+L +SLI+  KG+VELV Y + RDY+ I++GL G C + I+ +ATLVP  V+CLYDPS
Sbjct: 1197 KDALTISLIVGCKGSVELVSYRIVRDYDGIDNGLYGTCTVCILIIATLVPAAVRCLYDPS 1256

Query: 1216 MKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHLIE 1275
             +YAG+QNRNIMHLNP+SD+LRLLACIH+NENI +I+HLLN+SCPT  +PL +HI HLIE
Sbjct: 1257 RRYAGFQNRNIMHLNPSSDQLRLLACIHRNENITAILHLLNVSCPTPVSPLTVHIFHLIE 1316

Query: 1276 LSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHN 1335
            L GRTAP+FISHK   +  L++ SYS+ II+SFD+FERD++GTV VECFTA+SP  +MH+
Sbjct: 1317 LPGRTAPIFISHKQ-QDDPLDNHSYSRQIINSFDRFERDNEGTVYVECFTAVSPCTVMHD 1376

Query: 1336 DICTLALDKITSLIIMPFHRTWTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLG 1395
            ++CTLALDKI SLII+PFH TWTVDGF   DD  +R LN SVL +APCS+GIF DRG LG
Sbjct: 1377 EVCTLALDKIASLIILPFHITWTVDGFIDQDDQKIRKLNYSVLEKAPCSVGIFVDRGNLG 1436

Query: 1396 SIAATAIPRSRY---SVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPE-EHGQ 1455
               A           SVCV+F+GGKDDREAISYAKRM  DSRV LT+LRL  P E ++G 
Sbjct: 1437 RFRAMVSSSEMICSCSVCVIFVGGKDDREAISYAKRMVSDSRVRLTVLRLLAPMEDQNGS 1496

Query: 1456 KT-DNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRR 1494
            K+   WED +D EV++DF+SKCLGD RVVY E+VCRDGQ+TAFI+RKI + FDL+IVGRR
Sbjct: 1497 KSRQTWEDRVDGEVVKDFKSKCLGDERVVYLENVCRDGQETAFILRKIADRFDLIIVGRR 1556

BLAST of MS000462 vs. NCBI nr
Match: XP_022141218.1 (cation/H(+) antiporter 3-like [Momordica charantia])

HSP 1 Score: 1550.0 bits (4012), Expect = 0.0e+00
Identity = 782/785 (99.62%), Postives = 783/785 (99.75%), Query Frame = 0

Query: 1   MTFNSDLRITNSTVDIIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFF 60
           MTFNSDLRITNSTVDIIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFF
Sbjct: 5   MTFNSDLRITNSTVDIIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFF 64

Query: 61  LLMSIIHLLFKPFGVPKISSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYM 120
           LLMSIIHLLFKPFGVPKISSQIIAGLVVGCSWGPLDDTKF LFRINSEEILGLLSYFGYM
Sbjct: 65  LLMSIIHLLFKPFGVPKISSQIIAGLVVGCSWGPLDDTKFRLFRINSEEILGLLSYFGYM 124

Query: 121 LFLFITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIV 180
           LFLFITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIV
Sbjct: 125 LFLFITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIV 184

Query: 181 SIQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLG 240
           SIQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLG
Sbjct: 185 SIQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLG 244

Query: 241 YFNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQP 300
           YFNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQP
Sbjct: 245 YFNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQP 304

Query: 301 AVFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAA 360
           AVFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAA
Sbjct: 305 AVFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAA 364

Query: 361 FARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKI 420
           FARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKI
Sbjct: 365 FARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKI 424

Query: 421 INHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKN 480
           INHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKN
Sbjct: 425 INHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKN 484

Query: 481 EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL 540
           EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL
Sbjct: 485 EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL 544

Query: 541 SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ 600
           SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ
Sbjct: 545 SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ 604

Query: 601 EDNMLRNLNCSVLQKADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAI 660
           EDNMLRNLNCSVLQKADCSVAIFADKGHLGS ASMASSGTQFRYSVCLIYLGGSDDREAI
Sbjct: 605 EDNMLRNLNCSVLQKADCSVAIFADKGHLGSXASMASSGTQFRYSVCLIYLGGSDDREAI 664

Query: 661 SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC 720
           SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC
Sbjct: 665 SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC 724

Query: 721 DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR 780
           DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR
Sbjct: 725 DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR 784

Query: 781 TSVLI 786
           TSVL+
Sbjct: 785 TSVLV 789

BLAST of MS000462 vs. NCBI nr
Match: TYK31711.1 (cation/H(+) antiporter 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 795/1522 (52.23%), Postives = 1032/1522 (67.81%), Query Frame = 0

Query: 19   TTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKI 78
            +TIC+ +PP V S GIW     SEWWL  SLPLLE QL      ++I +   K FG+ KI
Sbjct: 13   STICIDLPPYVNSKGIWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRFGISKI 72

Query: 79   SSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAAKMDVRMILKT 138
            S QI+ GL  G SW   D+ K     + S+E+L LL+  GY  + F+ AAK+D+RM + T
Sbjct: 73   SCQILIGLAFGWSWNEWDEAKRKHLNVGSQEVLALLAELGYTFYTFLIAAKVDLRMTVAT 132

Query: 139  GRKAWIIGIPSTLIPIICGFWVGSYFLDG--LSPAEKNRVPVIVSIQSFISFPVIASLLS 198
            G+ A +IGI + L+P+I    V S  ++   L+  ++  +P + S  +  SFPV+ASL+ 
Sbjct: 133  GKSALLIGISALLLPLITETLVVSMVVEDWELTLRQRLALPALSSFHAKSSFPVVASLVK 192

Query: 199  ELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLIIF 258
            EL ++++ELGRLGLSSALI D+ G F+     QIR    N +        +M+   +  F
Sbjct: 193  ELHIMNSELGRLGLSSALINDIFGTFISTMQVQIRRYYLNTSSVSTELGALMMLILVAFF 252

Query: 259  VLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGA 318
            VLRP++ WI+KQTP+G PV      GV  L  L  V   L G  ++ G Y+LGLA PDGA
Sbjct: 253  VLRPTMFWIIKQTPQGMPVKSCYIDGVVSLAFLYIVLGNLTGHASIIGAYVLGLANPDGA 312

Query: 319  SGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVK 378
              L  +LV K+E  V + FMP+FV TC+L+AD+SK+        F ++NII+  V   VK
Sbjct: 313  P-LASTLVSKIESLVENVFMPIFVTTCALRADLSKISTTAFDVVFTKLNIILLCVACTVK 372

Query: 379  LVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTI 438
            LV     S YCKLP +D   LSLI+  KG VEL  Y V+   + I++ +   F++++L  
Sbjct: 373  LVASVSSSKYCKLPFKDALALSLIMCSKGPVELMAYTVSRDNRFIDNELFGCFVVWILFF 432

Query: 439  ATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSE-LRILPCIHKNEHIYGLIHLLNLSCP 498
            AT VP+ V  L DP R ++ +QNRNIMHL+  S+ LR+L CIH++E++  +IHLLNLSCP
Sbjct: 433  ATIVPIAVIRLYDPSRKYAGYQNRNIMHLNRFSDKLRLLACIHQHENVNAIIHLLNLSCP 492

Query: 499  TPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVY 558
            T +N + V+  HLIEL G+ TPIFISH+++      + YS+ +  SFD FE++N  G+ Y
Sbjct: 493  TIENSIVVHIFHLIELPGQITPIFISHKRQGNPFDKRSYSQQIIRSFDKFERENE-GTAY 552

Query: 559  AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 618
             ECFT ++P   M ND+C LALDK+ S IILPFH TWT DG I++ D  +R LN S+L++
Sbjct: 553  VECFTSVSPCTKMHNDVCTLALDKIASFIILPFHITWTMDGSIERVDKNVRTLNYSILER 612

Query: 619  ADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 678
            A CSV IFA +  L    +   S     YSVC+I+LGG DDREA+S+AKRM  +  +ELT
Sbjct: 613  APCSVGIFAHRRKLEHFKARKRSS----YSVCVIFLGGKDDREALSYAKRMVNDLRVELT 672

Query: 679  VLKLGSPMEDGSSSK----WENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLR 738
            VL+L +  +  + SK    WE+++D EV+KDFK  CLG+  V Y EEVC DG +TAL LR
Sbjct: 673  VLRLQTSKDYQNRSKSLNSWEHVMDEEVVKDFKEKCLGDERVVYEEEVCTDGQKTALILR 732

Query: 739  KIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTS--------- 798
            K+V+ FDLMIVGRR+GLE+PQT GL EWNEFPELG LGDLIAS D NT TS         
Sbjct: 733  KVVDMFDLMIVGRRNGLETPQTDGLDEWNEFPELGHLGDLIASSDINTGTSLLPSLPLLE 792

Query: 799  ----------------------------VLIGLIFGCSWRQFDKETINLFYERNQDILGL 858
                                        +L GL FG SW ++D+         +Q++L L
Sbjct: 793  FQLIVLCFSLAITYFFLKRLGISKISCQILTGLAFGWSWNEWDEAKRKNLNIGSQEVLAL 852

Query: 859  LAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDGSGISEA 918
            LAE GYTLY FL   K+DL MT+  GK AL+IGI ALL+PL     V S++V+   ++  
Sbjct: 853  LAELGYTLYTFLIAAKVDLRMTVATGKRALLIGISALLLPLITETLVVSMVVEDLALTLR 912

Query: 919  DLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFMMVTARQAK 978
                LP L SFH++ SFPV+ASL+ +L I+NSELGRL LSSAL SD +  F+++     K
Sbjct: 913  QRFALPSLSSFHAIISFPVVASLVKELHIMNSELGRLCLSSALISDFVGTFILIMKGHIK 972

Query: 979  RFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQAVIFLVLFTT 1038
            R+    S  S ++GAL++L L+  FV RP M WII+QTP+GMPVK  YI  V+FL L   
Sbjct: 973  RYHMNTSRISTEVGALMVLILVAFFVLRPTMFWIIKQTPQGMPVKSCYIDGVVFLALLYI 1032

Query: 1039 VLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITCALKADLSQI 1098
            VL  FTG  SI+G YV GLA+PDGAPLASTLV+  ECLV DVFMPI V TCAL+ADLS+I
Sbjct: 1033 VLGTFTGHASIIGAYVMGLAIPDGAPLASTLVSKFECLVEDVFMPIFVTTCALRADLSKI 1092

Query: 1099 S-SSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNKGAVELVLY 1158
            S ++FD  FTKLNI+L+ V   VK V+   +SR+C+LPF+D+LALSLIM +KG VEL+ Y
Sbjct: 1093 SATTFDVVFTKLNIILLCVACTVKFVASVSSSRYCKLPFKDALALSLIMCSKGPVELIFY 1152

Query: 1159 TMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMHLNPASDKL 1218
            T+ RD   I++ L G   + I+F AT+VPI VK LYDPS KYA YQ+RNIMHLN  SDKL
Sbjct: 1153 TIFRDNRFIDNELFGCFVVWILFFATMVPIAVKGLYDPSRKYACYQDRNIMHLNRFSDKL 1212

Query: 1219 RLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHLIELSGRTAPVFISHKYFHNKALN 1278
            RLLACIHQ+EN+N+IIHLLNLSCPT EN + +HI HLIEL GR  P+FISHK   N + +
Sbjct: 1213 RLLACIHQHENVNAIIHLLNLSCPTIENSIIVHIFHLIELPGRITPIFISHKRQGN-SFD 1272

Query: 1279 DRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITSLIIMPFHRT 1338
             RSYSQ I+HSFD+FE++++GT CVEC+T++SP  +MHND+CTLALDKI S II+PFH T
Sbjct: 1273 KRSYSQQIVHSFDKFEKENEGTACVECYTSVSPCTVMHNDVCTLALDKIASFIILPFHIT 1332

Query: 1339 WTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIAATAIPRSRYSVCVLFLGG 1398
            WTVDG     D  VRTLN S+L +APCS+GIF  R +L    A    RS YSVCV+FLGG
Sbjct: 1333 WTVDGSIGRVDKNVRTLNYSILEKAPCSVGIFVHRSKLEHFRAR--KRSSYSVCVIFLGG 1392

Query: 1399 KDDREAISYAKRMAKDSRVELTMLRLRGPP--EEHGQKTDNWEDILDAEVLRDFRSKCLG 1458
            KDDREA+SYAKRM KD RVELT+LRL+ P   +   +  ++WE I+D EV++DF+ KCLG
Sbjct: 1393 KDDREALSYAKRMVKDLRVELTVLRLKAPKNYQNRSKLHNSWEYIMDEEVVKDFKGKCLG 1452

Query: 1459 DGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRRNGLKSSQTDGLNEWNEFPELGVLG 1494
            D RVVY+E +C DGQ+TAF++RK+V++FDLMIVGRRNGL++ QTDGLNEWNEFPELG LG
Sbjct: 1453 DERVVYEEKICGDGQETAFLLRKVVDMFDLMIVGRRNGLETPQTDGLNEWNEFPELGYLG 1512

BLAST of MS000462 vs. NCBI nr
Match: KAA0025160.1 (cation/H(+) antiporter 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 800/1559 (51.31%), Postives = 1039/1559 (66.65%), Query Frame = 0

Query: 19   TTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKI 78
            +TIC+ +PP V S GIW     SEWWL  SLPLLE QL      ++I +   K FG+ KI
Sbjct: 13   STICIDLPPYVNSKGIWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRFGISKI 72

Query: 79   SSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAAKMDVRMILKT 138
            S QI+ GL  G SW   D+ K     + S+E+L LL+  GY L+ F+ AAK+D+RM + T
Sbjct: 73   SCQILIGLAFGWSWNEWDEAKRKHLNVGSQEVLALLAELGYTLYTFLIAAKVDLRMTVAT 132

Query: 139  GRKAWIIGIPSTLIPIICGFWVGSYFLDG--LSPAEKNRVPVIVSIQSFISFPVIASLLS 198
            G+ A +IGI + L+P+I    V S  ++   L+  ++  +P + S  +  SFPV+ASL+ 
Sbjct: 133  GKSALLIGISALLLPLITETLVVSMVVEDWELTLRQRLALPALSSFHAKSSFPVVASLVK 192

Query: 199  ELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLIIF 258
            EL ++++ELGRLGLSSAL+ D+ G F++    QIR    N +        +M+   +  F
Sbjct: 193  ELHIMNSELGRLGLSSALVNDIFGTFILTMQVQIRRYYLNTSSVSTELGALMMLILVAFF 252

Query: 259  VLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGA 318
            VLRP++ WI+KQTP+G PV      GV  L  L  V   + G  ++ G Y+LGLAIP+GA
Sbjct: 253  VLRPTMFWIIKQTPQGMPVKSCYIDGVVSLAFLYIVLGNITGHASIIGAYVLGLAIPNGA 312

Query: 319  SGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVK 378
              L  +LV K+EC V + FMP+FV TC+L+AD+SK+        F ++NII+  V   VK
Sbjct: 313  P-LASTLVSKIECLVENVFMPIFVTTCALRADLSKISTTAFDVVFTKLNIILLCVACTVK 372

Query: 379  LVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTI 438
            LV     S YCKLP +D   LSLI+  KG VEL  Y V+   + I++ +   F++++L  
Sbjct: 373  LVASVSSSKYCKLPFKDALALSLIMCSKGPVELMAYTVSRDNRFIDNELFGCFVVWVLFF 432

Query: 439  ATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSE-LRILPCIHKNEHIYGLIHLLNLSCP 498
            AT VP+ VK L DP R ++ +QNRNIMHL+  S+ LR+L CIH++E++  +IHLLNLSCP
Sbjct: 433  ATIVPIAVKRLYDPSRKYAGYQNRNIMHLNRFSDKLRLLACIHQHENVNAIIHLLNLSCP 492

Query: 499  TPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVY 558
            T +N + V+  HLIEL G+ TPIFISH+++      + YS+ +  SFD FE++N  G+ Y
Sbjct: 493  TIENSIVVHIFHLIELPGQITPIFISHKRQGNPFDKRSYSQQIIRSFDKFERENE-GTAY 552

Query: 559  AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 618
             ECFT ++P   M ND+C LALDK+ S IILPFH TWT DG I++ D  +R LN S+L++
Sbjct: 553  VECFTSVSPCTKMHNDVCTLALDKIASFIILPFHITWTVDGSIERVDKNVRTLNYSILER 612

Query: 619  ADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 678
            A CSV IFA +  L    +   S     YSVC+I+LGG DDREA+S+AKRM  +  +ELT
Sbjct: 613  APCSVGIFAHRRKLEHFKARKRSS----YSVCVIFLGGKDDREALSYAKRMVNDLRVELT 672

Query: 679  VLKLGSPMEDGSSSK----WENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLR 738
            VL+L +  +  + SK    WE+++D EV+KDFK  CLG+  V Y EEVC DG +TAL LR
Sbjct: 673  VLRLQASKDYQNRSKSLNSWEHVMDEEVVKDFKGKCLGDERVVYEEEVCTDGQKTALILR 732

Query: 739  KIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTS--------- 798
            K+V+ FDLMIVGRR+GLE+PQT GL EWNEFPELG LGDLIAS D NT TS         
Sbjct: 733  KVVDMFDLMIVGRRNGLETPQTDGLDEWNEFPELGHLGDLIASSDINTGTSLLETQLNPN 792

Query: 799  ------------------------------------------------------------ 858
                                                                        
Sbjct: 793  MTSTICIDIPTYVNSKGLWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRLGIS 852

Query: 859  -----VLIGLIFGCSWRQFDKETINLFYERNQDILGLLAEFGYTLYLFLSGVKIDLSMTI 918
                 +L GL FG SW ++D+         +Q++L LLAE GYTLY FL   K+DL MT+
Sbjct: 853  KISCQILTGLAFGWSWNEWDEAKRKNLNIGSQEVLALLAELGYTLYTFLIAAKVDLRMTV 912

Query: 919  RAGKNALIIGILALLVPLFAANFVRSLLVDGSGISEADLSTLPILISFHSMSSFPVIASL 978
              GK AL+IGI ALL+PL     V S++V+   ++      LP L SFH++ SFPV+ASL
Sbjct: 913  ATGKRALLIGISALLLPLITETLVVSMVVEDLALTLRQRFALPSLSSFHAIISFPVVASL 972

Query: 979  LSDLEIVNSELGRLGLSSALFSDLLSLFMMVTARQAKRFKDVPSIASLQLGALILLFLLI 1038
            + +L I+NSELGRL LSSAL SD +  F+++     KR+    S  S ++GAL++L L+ 
Sbjct: 973  VKELHIMNSELGRLCLSSALISDFVGTFILIMKGHIKRYHMNTSRISTEVGALMVLILVA 1032

Query: 1039 LFVFRPAMLWIIRQTPEGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPD 1098
             FV RPAM WII+QTP+GMPVK  YI  V+FL L   VL  FTG  SI+G YV GLA+PD
Sbjct: 1033 FFVLRPAMFWIIKQTPQGMPVKSCYIDGVVFLALLYIVLGTFTGHASIIGAYVMGLAIPD 1092

Query: 1099 GAPLASTLVNNIECLVSDVFMPILVITCALKADLSQIS-SSFDAAFTKLNIVLILVTFVV 1158
            GAPLASTLV+  ECLV DVFMPI V TCAL+ADLS+IS ++FD  FTKLNI+L+ V   V
Sbjct: 1093 GAPLASTLVSKFECLVEDVFMPIFVTTCALRADLSKISATTFDVVFTKLNIILLCVACTV 1152

Query: 1159 KIVSCFLTSRHCRLPFRDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVF 1218
            K V+   +SR+C+LPF+D+LALSLIM +KG VEL+ YT+ RD   I++ L G   + I+F
Sbjct: 1153 KFVASVSSSRYCKLPFKDALALSLIMCSKGPVELIFYTIFRDNRFIDNELFGCFVVWILF 1212

Query: 1219 VATLVPILVKCLYDPSMKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSC 1278
             AT+VPI VK LYDPS KYA YQ+RNIMHLN  SDKLRLLACIHQ+EN+N+IIHLLNLSC
Sbjct: 1213 FATMVPIAVKGLYDPSRKYACYQDRNIMHLNRFSDKLRLLACIHQHENVNAIIHLLNLSC 1272

Query: 1279 PTAENPLAIHILHLIELSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTV 1338
            PT EN + +HI HLIEL GR  P+FISHK   N + + RSYSQ I+HSFD+FER+++GT 
Sbjct: 1273 PTIENSIIVHIFHLIELPGRITPIFISHKRQGN-SFDKRSYSQQIVHSFDKFERENEGTA 1332

Query: 1339 CVECFTAISPNRLMHNDICTLALDKITSLIIMPFHRTWTVDGFAYHDDNMVRTLNCSVLG 1398
            CVEC+T++SP  +MHND+CTLALDKI S II+PFH TWTVDG     D  VRTLN S+L 
Sbjct: 1333 CVECYTSVSPCTVMHNDVCTLALDKIASFIILPFHITWTVDGSIGRVDKNVRTLNYSILE 1392

Query: 1399 RAPCSIGIFADRGRLGSIAATAIPRSRYSVCVLFLGGKDDREAISYAKRMAKDSRVELTM 1458
            RAPCS+GIFA R +L    A    RS YSVCV+FLGGKDDREA+SYAKRM KD RVELT+
Sbjct: 1393 RAPCSVGIFAHRSKLEHFRAR--KRSSYSVCVIFLGGKDDREALSYAKRMVKDLRVELTV 1452

Query: 1459 LRLRGPP--EEHGQKTDNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRK 1494
            LRL+ P   +   +  ++WE I+D EV++DF+ KCLGD RVVY+E +C DGQ+TAF++RK
Sbjct: 1453 LRLKAPQNYQNRSKLHNSWEYIMDEEVVKDFKGKCLGDERVVYEEKICGDGQETAFLLRK 1512

BLAST of MS000462 vs. NCBI nr
Match: XP_008439404.2 (PREDICTED: uncharacterized protein LOC103484221 [Cucumis melo])

HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 794/1574 (50.44%), Postives = 1035/1574 (65.76%), Query Frame = 0

Query: 19   TTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGV--- 78
            +TIC+ +PP V S GIW     SEWWL  SLPLLE QL      ++I +   K F +   
Sbjct: 13   STICIDLPPYVNSKGIWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRFAIKPD 72

Query: 79   ------------PKISSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFL 138
                          ++  +  GL  G SW   D+ K     + S+E+L LL+  GY L+ 
Sbjct: 73   GKIQKNLNTIYSKNLNPVLRIGLAFGWSWNEWDEAKRKHLNVGSQEVLALLAELGYTLYT 132

Query: 139  FITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDG--LSPAEKNRVPVIVS 198
            F+ AAK+D+RM + TG+ A +IGI + L+P+I    V S  ++   L+  ++  +P + S
Sbjct: 133  FLIAAKVDLRMTVATGKSALLIGISALLLPLITETLVVSMVVEDWELTLRQRLALPALSS 192

Query: 199  IQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGY 258
              +  SFPV+ASL+ EL ++++ELGRLGLSSAL+ D+ G F++    QIR    N +   
Sbjct: 193  FHAKSSFPVVASLVKELHIMNSELGRLGLSSALVNDIFGTFILTMQVQIRRYYLNTSSVS 252

Query: 259  FNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPA 318
                 +M+   +  FVLRP++ WI+KQTP+G PV      GV  L  L  V   + G  +
Sbjct: 253  TELGALMMLILVAFFVLRPTMFWIIKQTPQGMPVKSCYIDGVVSLAFLYIVLGNITGHAS 312

Query: 319  VFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAF 378
            + G Y+LGLAIP+GA  L  +LV K+EC V + FMP+FV TC+L+AD+SK+        F
Sbjct: 313  IIGAYVLGLAIPNGAP-LASTLVSKIECLVENVFMPIFVTTCALRADLSKISTTAFDVVF 372

Query: 379  ARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKII 438
             ++NII+  V   VKLV     S YCKLP +D   LSLI+  KG VEL  Y V+   + I
Sbjct: 373  TKLNIILLCVACTVKLVASVSSSKYCKLPFKDALALSLIMCSKGPVELMAYTVSRDNRFI 432

Query: 439  NHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSE-LRILPCIHKN 498
            ++ +   F++++L  AT VP+ VK L DP R ++ +QNRNIMHL+  S+ LR+L CIH++
Sbjct: 433  DNELFGCFVVWVLFFATIVPIAVKRLYDPSRKYAGYQNRNIMHLNRFSDKLRLLACIHQH 492

Query: 499  EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL 558
            E++  +IHLLNLSCPT +N + V+  HLIEL G+ TPIFISH+++      + YS+ +  
Sbjct: 493  ENVNAIIHLLNLSCPTIENSIVVHIFHLIELPGQITPIFISHKRQGNPFDKRSYSQQIIR 552

Query: 559  SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ 618
            SFD FE++N  G+ Y ECFT ++P   M ND+C LALDK+ S IILPFH TWT DG I++
Sbjct: 553  SFDKFERENE-GTAYVECFTSVSPCTKMHNDVCTLALDKIASFIILPFHITWTVDGSIER 612

Query: 619  EDNMLRNLNCSVLQKADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAI 678
             D  +R LN S+L++A CSV IFA +  L    +   S     YSVC+I+LGG DDREA+
Sbjct: 613  VDKNVRTLNYSILERAPCSVGIFAHRRKLEHFKARKRSS----YSVCVIFLGGKDDREAL 672

Query: 679  SFAKRMAKEPTIELTVLKLGSPMEDGSSSK----WENMLDSEVLKDFKMNCLGNGGVRYF 738
            S+AKRM  +  +ELTVL+L +  +  + SK    WE+++D EV+KDFK  CLG+  V Y 
Sbjct: 673  SYAKRMVNDLRVELTVLRLQASKDYQNRSKSLNSWEHVMDEEVVKDFKGKCLGDERVVYE 732

Query: 739  EEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLD 798
            EEVC DG +TAL LRK+V+ FDLMIVGRR+GLE+PQT GL EWNEFPELG LGDLIAS D
Sbjct: 733  EEVCTDGQKTALILRKVVDMFDLMIVGRRNGLETPQTDGLDEWNEFPELGHLGDLIASSD 792

Query: 799  NNTRTS------------------------------------------------------ 858
             NT TS                                                      
Sbjct: 793  INTGTSLLETQLNPNMTSTICIDIPTYVNSKGLWVEFDDSEWWLKPSLPLLEFQLIVLCF 852

Query: 859  --------------------VLIGLIFGCSWRQFDKETINLFYERNQDILGLLAEFGYTL 918
                                +L GL FG SW ++D+         +Q++L LLAE GYTL
Sbjct: 853  SLAITYFFLKRLGISKISCQILTGLAFGWSWNEWDEAKRKNLNIGSQEVLALLAELGYTL 912

Query: 919  YLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDGSGISEADLSTLPIL 978
            Y FL   K+DL MT+  GK AL+IGI ALL+PL     V S++V+   ++      LP L
Sbjct: 913  YTFLIAAKVDLRMTVATGKRALLIGISALLLPLITETLVVSMVVEDLALTLRQRFALPSL 972

Query: 979  ISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFMMVTARQAKRFKDVPSI 1038
             SFH++ SFPV+ASL+ +L I+NSELGRL LSSAL SD +  F+++     KR+    S 
Sbjct: 973  SSFHAIISFPVVASLVKELHIMNSELGRLCLSSALISDFVGTFILIMKGHIKRYHMNTSR 1032

Query: 1039 ASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQAVIFLVLFTTVLFNFTGQ 1098
             S ++GAL++L L+  FV RPAM WII+QTP+GMPVK  YI  V+FL L   VL  FTG 
Sbjct: 1033 ISTEVGALMVLILVAFFVLRPAMFWIIKQTPQGMPVKSCYIDGVVFLALLYIVLGTFTGH 1092

Query: 1099 ISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITCALKADLSQIS-SSFDAA 1158
             SI+G YV GLA+PDGAPLASTLV+  ECLV DVFMPI V TCAL+ADLS+IS ++FD  
Sbjct: 1093 ASIIGAYVMGLAIPDGAPLASTLVSKFECLVEDVFMPIFVTTCALRADLSKISATTFDVV 1152

Query: 1159 FTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNKGAVELVLYTMARDYNA 1218
            FTKLNI+L+ V   VK V+   +SR+C+LPF+D+LALSLIM +KG VEL+ YT+ RD   
Sbjct: 1153 FTKLNIILLCVACTVKFVASVSSSRYCKLPFKDALALSLIMCSKGPVELIFYTIFRDNRF 1212

Query: 1219 INDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMHLNPASDKLRLLACIHQ 1278
            I++ L G   + I+F AT+VPI VK LYDPS KYA YQ+RNIMHLN  SDKLRLLACIHQ
Sbjct: 1213 IDNELFGCFVVWILFFATMVPIAVKGLYDPSRKYACYQDRNIMHLNRFSDKLRLLACIHQ 1272

Query: 1279 NENINSIIHLLNLSCPTAENPLAIHILHLIELSGRTAPVFISHKYFHNKALNDRSYSQHI 1338
            +EN+N+IIHLLNLSCPT EN + +HI HLIEL GR  P+FISHK   N + + RSYSQ I
Sbjct: 1273 HENVNAIIHLLNLSCPTIENSIIVHIFHLIELPGRITPIFISHKRQGN-SFDKRSYSQQI 1332

Query: 1339 IHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITSLIIMPFHRTWTVDGFAY 1398
            +HSFD+FER+++GT CVEC+T++SP  +MHND+CTLALDKI S II+PFH TWTVDG   
Sbjct: 1333 VHSFDKFERENEGTACVECYTSVSPCTVMHNDVCTLALDKIASFIILPFHITWTVDGSIG 1392

Query: 1399 HDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIAATAIPRSRYSVCVLFLGGKDDREAIS 1458
              D  VRTLN S+L RAPCS+GIFA R +L    A    RS YSVCV+FLGGKDDREA+S
Sbjct: 1393 RVDKNVRTLNYSILERAPCSVGIFAHRSKLEHFRAR--KRSSYSVCVIFLGGKDDREALS 1452

Query: 1459 YAKRMAKDSRVELTMLRLRGPP--EEHGQKTDNWEDILDAEVLRDFRSKCLGDGRVVYKE 1494
            YAKRM KD RVELT+LRL+ P   +   +  ++WE I+D EV++DF+ KCLGD RVVY+E
Sbjct: 1453 YAKRMVKDLRVELTVLRLKAPQNYQNRSKLHNSWEYIMDEEVVKDFKGKCLGDERVVYEE 1512

BLAST of MS000462 vs. ExPASy Swiss-Prot
Match: Q9FYC1 (Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 5.6e-140
Identity = 290/708 (40.96%), Postives = 429/708 (60.59%), Query Frame = 0

Query: 809  QDILGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDG 868
            + + GL+    Y ++ FL GVK+DLS+    G+ A+ IG+ ++L+ +     +  L++  
Sbjct: 115  ETLFGLVGACSYMMFWFLMGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRD 174

Query: 869  SGISEAD--LSTLPILISF--HSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSL 928
             G  + +  +S   I+  +    +SSFPVI +LL +L + NSELGRL +SSA+ SD  + 
Sbjct: 175  VGTKKGEPVMSFFEIIFIYLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTS 234

Query: 929  FMMVTARQAKRFKD--------------VPSIASLQLGALILLFLLILFVFRPAMLWIIR 988
             +       K  KD              V +    + G ++L     +++FRP M +II+
Sbjct: 235  ILSAVLVFLKELKDDKSRLGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIK 294

Query: 989  QTPEGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIE 1048
            +TP G PVK  YI A+I LV  + +L ++  Q   +GP++ GLAVP G PL S ++   E
Sbjct: 295  RTPSGRPVKKFYIYAIIILVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFE 354

Query: 1049 CLVSDVFMPILVITCALKADLSQISSSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRL 1108
             +V   F+P  V T A + D S + S  D    K  ++L+ V+F+VK     L +    +
Sbjct: 355  SVVFGTFLPFFVATSAEEIDTSILQSWID---LKSIVILVSVSFIVKFALTTLPAFLYGM 414

Query: 1109 PFRDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYD 1168
            P +D +ALSLIMS KG  E   Y  A     I         L I+  + ++P L+K +YD
Sbjct: 415  PAKDCIALSLIMSFKGIFEFGAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYD 474

Query: 1169 PSMKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHL 1228
            PS  YAGY+ RN++H+ P S+ LR+L+CI++ ++I  +I+LL  +CP+ ENP+A ++LHL
Sbjct: 475  PSRMYAGYEKRNMLHMKPNSE-LRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHL 534

Query: 1229 IELSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLM 1288
            +EL G+  PV ISH+    K+ N    S++++ SF+QF  D  G+V V  +TA+S  ++M
Sbjct: 535  MELVGQANPVLISHRLQTRKSENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMM 594

Query: 1289 HNDICTLALDKITSLIIMPFHRTWTVDGFAYHDDN-MVRTLNCSVLGRAPCSIGIFADRG 1348
            H DIC LAL+  TSLII+PFH+TW+ DG A   D+ M+R LN SVL  +PCS+GIF  R 
Sbjct: 595  HGDICMLALNNTTSLIILPFHQTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRS 654

Query: 1349 RLG--SIAATAIPRSRYSVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPEEHG 1408
              G  +I  TA   S Y VC+LFLGGKDDREA+S AKRMA+DSR+ +T++ L    +   
Sbjct: 655  SNGRRTIKETAANFSSYQVCMLFLGGKDDREALSLAKRMARDSRITITVVSLISSEQRAN 714

Query: 1409 QKTDNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRR 1468
            Q TD W+ +LD E+LRD +S  L    +V+ E+V  D  QT+ +++ I N +DL IVGR 
Sbjct: 715  QATD-WDRMLDLELLRDVKSNVLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGRE 774

Query: 1469 NGLKSSQTDGLNEWNEFPELGVLGDLIASTDINSRASLLVIQQQQIVI 1496
             G KS  T+GL EW+EF ELG++GDL+ S D+N +AS+LVIQQQQ +I
Sbjct: 775  KGRKSVFTEGLEEWSEFEELGIIGDLLTSQDLNCQASVLVIQQQQQMI 817

BLAST of MS000462 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 8.6e-133
Identity = 280/715 (39.16%), Postives = 429/715 (60.00%), Query Frame = 0

Query: 811  ILGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAAN--FVRSLLVDG 870
            +  L A   Y ++ FL GVK+D  +    G+ A+ IG+ ++L+     +  F  +L   G
Sbjct: 118  VFSLTAACSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVG 177

Query: 871  SGISEADLSTLP--ILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFM 930
            +  S+  L++L   ++ S   +SSFPV+ +LL +L + NSELGRL +SSA+ SD  +  +
Sbjct: 178  TKNSDHTLNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSIL 237

Query: 931  MVTARQAKRFKDVPS----------IAS----LQLGALILLFLLILFVFRPAMLWIIRQT 990
                   K  KD  +          IA     ++ G ++L   + ++VFRP M +II+QT
Sbjct: 238  ASVLIFMKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQT 297

Query: 991  PEGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECL 1050
            P G PVK  Y+  +I +V  + +L N+  Q   MGP++ GLAVP G PL S ++   E  
Sbjct: 298  PSGRPVKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESA 357

Query: 1051 VSDVFMPILVITCALKADLSQISSSFDAAFTKLN-IVLILVT-FVVKIVSCFLTSRHCRL 1110
            +   F+P  + + + + D+S +       +  LN I+LI+VT FVVK +   + +    +
Sbjct: 358  IFGTFLPFFIASSSTEIDISAL-----FGWEGLNGIILIMVTSFVVKFIFTTVPALFYGM 417

Query: 1111 PFRDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYD 1170
            P  D  ALSLIMS KG  EL  Y +A    ++         L I   + ++P +++ LYD
Sbjct: 418  PMEDCFALSLIMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYD 477

Query: 1171 PSMKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHL 1230
            PS  YAGY+ RN+ HL P S+ LR+L+CI++ ++I+ +I+LL   CP+ E+P+A ++LHL
Sbjct: 478  PSRMYAGYEKRNMQHLKPNSE-LRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHL 537

Query: 1231 IELSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLM 1290
            +EL G+  P+FISHK    +   + SYS +++ SF++F +D  G+V V  +TA+S    M
Sbjct: 538  MELVGQANPIFISHK-LQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTM 597

Query: 1291 HNDICTLALDKITSLIIMPFHRTWTVDGFA-YHDDNMVRTLNCSVLGRAPCSIGIFADRG 1350
            H DIC LAL+  TSLI++PFH+TW+ DG A   ++NM+R LN SVL  APCS+G+F  R 
Sbjct: 598  HGDICMLALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRS 657

Query: 1351 RLG---------SIAATAIPRSRYSVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLR 1410
              G         +I  T    S Y++C++FLGGKDDREA++ A RMA+D R+ +T++RL 
Sbjct: 658  SSGRKNISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLI 717

Query: 1411 GPPEEHGQKTDNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFD 1470
               +E  ++   W+ +LD E+LRD +S  L D  + Y E    D  +T+ ++R +V+ FD
Sbjct: 718  -TTDEKARENTVWDKMLDDELLRDVKSNTLVD--IFYSEKAIEDAAETSSLLRSMVSDFD 777

Query: 1471 LMIVGRRNGLKSSQTDGLNEWNEFPELGVLGDLIASTDINSRASLLVIQQQQIVI 1496
            + IVGR NG  S  T+GL EW+EF ELG++GDL+ S D N +AS+LVIQQQQ++I
Sbjct: 778  MFIVGRGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQQQLMI 822

BLAST of MS000462 vs. ExPASy Swiss-Prot
Match: Q9FYB9 (Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2)

HSP 1 Score: 436.8 bits (1122), Expect = 9.9e-121
Identity = 266/687 (38.72%), Postives = 397/687 (57.79%), Query Frame = 0

Query: 812  LGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLL--VDGS 871
            L  ++ FG  ++ FL  V+    +   +GK  ++IGI++   PLF+ +F+      +D  
Sbjct: 103  LRCISVFGRLMFTFLMTVRTSRRVAFHSGKLPVVIGIVSFFAPLFSLSFLNLFTDNIDPH 162

Query: 872  GIS-EADLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFMMV 931
             +S +  L+   +++   S    P    +L +L+I+NSELGRL LS++  +D+L +F M+
Sbjct: 163  YMSLDKALAERTVIVITQSQILLPSTTYILLELKIINSELGRLALSASAINDMLGIFAMI 222

Query: 932  TA-RQAKRFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQAVI 991
             A  QA       +IA   L A+I+ FL++ FVF+P + WII +TPE  PV+  YI AVI
Sbjct: 223  VATTQATYIHVSHAIAYRDLVAVIIFFLIVFFVFKPMVQWIIDRTPEDKPVEDIYIHAVI 282

Query: 992  FLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITCAL 1051
                 +   F F     ++GP + G+ +P+G PL S L    E L  +VF+PI +   A+
Sbjct: 283  LTAFASAAYFVFFNMKYVLGPLIIGIIIPEGPPLGSALEAKFERLTMNVFLPISITFSAM 342

Query: 1052 KADLSQISSSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNKGA 1111
            + D  +I S F   +   NI L L+  V+K+V+C     + +LP  +SLA+SLI+S K  
Sbjct: 343  RCDGLRILSQFTDIY--FNIFLTLLILVIKLVACLTLCLYYKLPRSESLAVSLILSYKSF 402

Query: 1112 VELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMHLN 1171
            VE VLY    +   I+     +  L  +  A +VP++V+ +YDP  KY  YQ R+I+HL 
Sbjct: 403  VEFVLYEAVLEEKFISQATYAFLILYSLLSAGIVPMVVRSMYDPKRKYVNYQKRDILHLE 462

Query: 1172 PASDKLRLLACIHQNENINSIIHLLNL-SCPTAENPLAIHILHLIELSGRTAPVFISHKY 1231
             A+  LR+L C+H+ EN++  I  L L S P  + P+A+ +LHL++L G+  P+ +SH  
Sbjct: 463  -ANSGLRILTCLHKPENVSETIAFLQLFSSPIHDFPIAVTVLHLVKLVGQINPIIVSHDK 522

Query: 1232 FHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITSLI 1291
               K L+  SY      +F QF ++S  +V V  FTA S   LMH DICTLALD+ TS+I
Sbjct: 523  -KLKRLHKNSYIHTANLAFRQFMQESLESVTVTTFTAFSHENLMHEDICTLALDRTTSMI 582

Query: 1292 IMPFHRTWTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIA-ATAIPRSRYS 1351
            ++P  R WTVDG    DD   R LN S+L RAPCSIGI  DRG+    +  T+  R    
Sbjct: 583  VVPSGRKWTVDGMFESDDLAARQLNQSLLDRAPCSIGILVDRGQFSRKSYVTSKNRYNID 642

Query: 1352 VCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPEEHGQKTDNWEDILDAEVLRDF 1411
            V VLF+GGKDDREA+S  KRM  + RV +T++RL    E   +    W+ ILD E L+D 
Sbjct: 643  VGVLFIGGKDDREALSLVKRMKYNPRVRVTVIRLIFDHEIESE----WDYILDNEGLKDL 702

Query: 1412 RSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRRNGLKSSQTDGLNEWNEFP 1471
            +S    +  ++Y E +     +    ++ +   +DLM+VGR + + S    GL EW E P
Sbjct: 703  KSTESNED-ILYTERIVTSVVEVVKAVQLLAEEYDLMVVGRDHDMTSQDLSGLTEWVELP 762

Query: 1472 ELGVLGDLIASTDINSRASLLVIQQQQ 1493
            ELGV+GDL+A+ D+NS+ S+LV+QQQQ
Sbjct: 763  ELGVIGDLLAARDLNSKVSVLVVQQQQ 780

BLAST of MS000462 vs. ExPASy Swiss-Prot
Match: Q9FYC0 (Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2)

HSP 1 Score: 428.3 bits (1100), Expect = 3.5e-118
Identity = 267/785 (34.01%), Postives = 414/785 (52.74%), Query Frame = 0

Query: 22  CLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKISSQ 81
           C+ +   + S G W N  + +     SLPL+E Q+   F+ + IIH   K FG+  I S 
Sbjct: 10  CIPLVFNISSFGFWENLKSPDVIFGYSLPLMEFQILLIFVFIIIIHSFLKSFGISPIPSY 69

Query: 82  IIAGLVVG-------------CSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAA 141
           ++AGL++G              SW P          ++    L  LS  G ++  F    
Sbjct: 70  MLAGLILGPQLFNLREVSSRKLSWDP---------ALDGNGPLRGLSVCGNIMLAFFMTV 129

Query: 142 KMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSP--AEKNRV----PVIVSI 201
           K+  R+    G    +IG  S ++P + GF V +   D + P     N+V     V++S 
Sbjct: 130 KISRRLAFNNGWLPIVIGTLSFIVPFLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISS 189

Query: 202 QSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRS-NITLGY 261
           QS I  P +   LSELK++++ELGRL LS++LI D+    V   A  +   ++ +    Y
Sbjct: 190 QSSILLPTVVHFLSELKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNISPMTAY 249

Query: 262 FNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPA 321
            +   +++   +   VLRP V WI+++TPEGKPV+    H V + V+ SA  S+      
Sbjct: 250 RDLIAVIILILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMKY 309

Query: 322 VFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAF 381
           + GP+LLG+ IP+G   +G +L  K E    +  +P+ +   +++ DV K+   +    +
Sbjct: 310 LLGPFLLGIIIPEGPP-IGSALEAKYEALTMNVLIPISITFSTMRCDVMKIVYQYDDIWY 369

Query: 382 ARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKII 441
              NI +   T  +K+    +  LYCK+P ++    SL+L  K   E+  Y  T+    I
Sbjct: 370 ---NIFLMTFTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDSYI 429

Query: 442 NHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNE 501
           +     + I   L  +  +P  +  L DPKR +  +Q +NIM+L P+S+LRIL CIH+ E
Sbjct: 430 SQATYTFLITCALINSGIIPTALAGLYDPKRKYVGYQKKNIMNLKPDSDLRILTCIHRPE 489

Query: 502 HIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLS 561
           +I   I  L     T    + V  LHL++LVG+T P+ ISH ++   +    Y     L+
Sbjct: 490 NISAAISFLQFLPST----IVVTVLHLVKLVGKTVPVLISHNKQINRVVTNSYIHTANLA 549

Query: 562 FDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQE 621
           F   E      SV    FT IT    M ++ICK+AL++ TS+II+P  R WT DG  + E
Sbjct: 550 FSQLE------SVTMTMFTAITHENLMHDEICKVALEQATSIIIVPSGRKWTVDGAFESE 609

Query: 622 DNMLRNLNCSVLQKADCSVAIFADKGHLGSIASMASSGT-QFRYSVCLIYLGGSDDREAI 681
           D  +R LN S+L+ A CS+ I  D+G L      +  GT +F   V +I++GG DDREA+
Sbjct: 610 DEAIRRLNESLLKSASCSIGILVDRGQL------SLKGTRKFNIDVGVIFIGGKDDREAL 669

Query: 682 SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC 741
           S  K+M + P +++TV++L S  E   S+ W+ +LD EVL+D K +      + Y E + 
Sbjct: 670 SLVKKMKQNPRVKITVIRLISDRET-ESTNWDYILDHEVLEDLK-DTEATNSIAYTERIV 729

Query: 742 DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR 786
             GP+ A T+R +  ++DLM+VGR  G+ SP   GL EW E PELGV+GDL+AS + ++R
Sbjct: 730 TGGPEVATTVRSLSEDYDLMVVGRDHGMASPDFDGLMEWVELPELGVIGDLLASRELDSR 763

BLAST of MS000462 vs. ExPASy Swiss-Prot
Match: Q58P69 (Cation/H(+) antiporter 10 OS=Arabidopsis thaliana OX=3702 GN=CHX10 PE=2 SV=2)

HSP 1 Score: 420.6 bits (1080), Expect = 7.3e-116
Identity = 263/690 (38.12%), Postives = 393/690 (56.96%), Query Frame = 0

Query: 812  LGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDG--- 871
            L  ++ FG  ++ FL  V+    +   +GK  ++IGI++   PLF   F ++   D    
Sbjct: 103  LRCISVFGTLMFTFLMTVRTSRRVAFHSGKLPVVIGIVSFFAPLFGLGF-QNFFSDNIDP 162

Query: 872  --SGISEADLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFM 931
                +++A L     ++   S    P    +L +L+I+NSELGRL LS+ + +D+L +F 
Sbjct: 163  HYMPLTKA-LGERTAIVITQSSILLPSTTYILLELKIINSELGRLALSACVINDILGIFS 222

Query: 932  MVTAR-QAKRFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQA 991
            M+ A  QA       + A     A+I+ FL++  VF+P + W+I +TPE  PV+  YI A
Sbjct: 223  MIVASIQATYIHVSHATAYRDTVAVIIFFLVVFLVFKPMVQWVIDRTPEDKPVEDMYIHA 282

Query: 992  VIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITC 1051
            VI   L +   F F     I+GP + G+ +P+G PL S L    E L  +VF+PI +   
Sbjct: 283  VIITALASAAYFVFFNMKYILGPLMIGIIIPEGPPLGSALEAKFERLTMNVFLPISITFS 342

Query: 1052 ALKADLSQISSSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNK 1111
            A++ D ++I S F+  F   NI L  +  V+K+V+C     + +LP  +SLA+S I+S K
Sbjct: 343  AMRCDGARILSQFNDIF--FNIFLTFLILVIKLVACLAPCLYYKLPLSESLAVSFILSYK 402

Query: 1112 GAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMH 1171
               + VLY    D   I+     +  L  +  A +VP +++ +YDP  KY  YQ R+I+H
Sbjct: 403  SFADFVLYEAVLDDTYISQATYSFLILYSLLNAGIVPTVLRRMYDPRRKYVNYQKRDILH 462

Query: 1172 LNPASDKLRLLACIHQNENINSIIHLLN-LSCPTAENPLAIHILHLIELSGRTAPVFISH 1231
            L   SD LR+L C+H+ EN++  I  L  LS P  + P+A+ +LHL++L G+  P+ +SH
Sbjct: 463  LERNSD-LRILTCLHKPENVSETIAFLQLLSSPNLDFPIAVTVLHLVKLVGQINPIIVSH 522

Query: 1232 KYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITS 1291
                 K LN  SY      +F QF  +S  +V V  FTA S   LMH DICTLALDK TS
Sbjct: 523  DK-KLKRLNKDSYIHTANLAFRQFVLESLESVTVTTFTAFSHENLMHEDICTLALDKTTS 582

Query: 1292 LIIMPFHRTWTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIAATAIPRSRY 1351
            +I++P  R WTVDG    D+  +R LN S+L RAPCSIGI  DRG+  S  +    + RY
Sbjct: 583  MIVVPSGRKWTVDGLFESDNTAIRHLNQSLLDRAPCSIGILVDRGQF-SRKSIVTSKKRY 642

Query: 1352 --SVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPEEHGQKTDNWEDILDAEVL 1411
               V VLF+GGKDDREA+S  KRM  + R+ +T++RL     +H  ++D W+ ILD E L
Sbjct: 643  IIDVGVLFIGGKDDREALSLVKRMKNNPRIRVTVIRL---VFDHEIESD-WDYILDNEGL 702

Query: 1412 RDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRRNGLKSSQTDGLNEWN 1471
            +D +S    +  + Y E +     +    ++ +   +DLM+VGR + + S    GL EW 
Sbjct: 703  KDLKS-TEDNKDIDYIERIVTSSVEVVKAVQLLAEEYDLMVVGRDHDMTSQDLSGLMEWV 762

Query: 1472 EFPELGVLGDLIASTDINSRASLLVIQQQQ 1493
            E PELGV+GDL+A+ D++S+ S+LV+QQQQ
Sbjct: 763  ELPELGVIGDLLAARDLSSKVSVLVVQQQQ 780


HSP 2 Score: 417.9 bits (1073), Expect = 4.7e-115
Identity = 259/770 (33.64%), Postives = 412/770 (53.51%), Query Frame = 0

Query: 29  VGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKISSQIIAGLVV 88
           + S G W N  + +     SLPLLE+Q+   F  + + H+  +  G+ +I+S +IAG+V+
Sbjct: 17  ISSQGFWDNLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGISQIASYMIAGIVL 76

Query: 89  GCS-WGPLDDTKFILF---RINSEEILGLLSYFGYMLFLFITAAKMDVRMILKTGRKAWI 148
           G   +  L+ +   L     ++    L  +S FG ++F F+   +   R+   +G+   +
Sbjct: 77  GPQLFDVLEKSSGKLSVDPALDGIAALRCISVFGTLMFTFLMTVRTSRRVAFHSGKLPVV 136

Query: 149 IGIPSTLIPIICGFWVGSYFLDGLSP-------AEKNRVPVIVSIQSFISFPVIASLLSE 208
           IGI S   P+  G    ++F D + P       A   R  ++++ QS I  P    +L E
Sbjct: 137 IGIVSFFAPLF-GLGFQNFFSDNIDPHYMPLTKALGERTAIVIT-QSSILLPSTTYILLE 196

Query: 209 LKMVSTELGRLGLSSALIADLLGQFVMATAN-QIRISRSNITLGYFNKAGIMVQFCLIIF 268
           LK++++ELGRL LS+ +I D+LG F M  A+ Q      +    Y +   +++ F ++  
Sbjct: 197 LKIINSELGRLALSACVINDILGIFSMIVASIQATYIHVSHATAYRDTVAVIIFFLVVFL 256

Query: 269 VLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGA 328
           V +P V W++ +TPE KPV     H V I  L SA          + GP ++G+ IP+G 
Sbjct: 257 VFKPMVQWVIDRTPEDKPVEDMYIHAVIITALASAAYFVFFNMKYILGPLMIGIIIPEGP 316

Query: 329 SGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVK 388
             LG +L  K E    + F+P+ +   +++ D +++   F    F   NI +T +   +K
Sbjct: 317 P-LGSALEAKFERLTMNVFLPISITFSAMRCDGARILSQFNDIFF---NIFLTFLILVIK 376

Query: 389 LVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTI 448
           LV C    LY KLPL +   +S ILS K   +   Y        I+    ++ IL+ L  
Sbjct: 377 LVACLAPCLYYKLPLSESLAVSFILSYKSFADFVLYEAVLDDTYISQATYSFLILYSLLN 436

Query: 449 ATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNEHIYGLIHLLN-LSCP 508
           A  VP  ++ + DP+R + ++Q R+I+HL  NS+LRIL C+HK E++   I  L  LS P
Sbjct: 437 AGIVPTVLRRMYDPRRKYVNYQKRDILHLERNSDLRILTCLHKPENVSETIAFLQLLSSP 496

Query: 509 TPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVY 568
               P+AV  LHL++LVG+  PI +SH ++ K +    Y     L+F  F  + S  SV 
Sbjct: 497 NLDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLNKDSYIHTANLAFRQFVLE-SLESVT 556

Query: 569 AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 628
              FT  +    M  DIC LALDK TS+I++P  R WT DGL + ++  +R+LN S+L +
Sbjct: 557 VTTFTAFSHENLMHEDICTLALDKTTSMIVVPSGRKWTVDGLFESDNTAIRHLNQSLLDR 616

Query: 629 ADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 688
           A CS+ I  D+G   S  S+ +S  ++   V ++++GG DDREA+S  KRM   P I +T
Sbjct: 617 APCSIGILVDRGQF-SRKSIVTSKKRYIIDVGVLFIGGKDDREALSLVKRMKNNPRIRVT 676

Query: 689 VLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVN 748
           V++L    +    S W+ +LD+E LKD K +   N  + Y E +     +    ++ +  
Sbjct: 677 VIRL--VFDHEIESDWDYILDNEGLKDLK-STEDNKDIDYIERIVTSSVEVVKAVQLLAE 736

Query: 749 EFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLI 786
           E+DLM+VGR   + S   +GL EW E PELGV+GDL+A+ D +++ SVL+
Sbjct: 737 EYDLMVVGRDHDMTSQDLSGLMEWVELPELGVIGDLLAARDLSSKVSVLV 775

BLAST of MS000462 vs. ExPASy TrEMBL
Match: A0A6J1CJU7 (cation/H(+) antiporter 3-like OS=Momordica charantia OX=3673 GN=LOC111011670 PE=4 SV=1)

HSP 1 Score: 1550.0 bits (4012), Expect = 0.0e+00
Identity = 782/785 (99.62%), Postives = 783/785 (99.75%), Query Frame = 0

Query: 1   MTFNSDLRITNSTVDIIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFF 60
           MTFNSDLRITNSTVDIIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFF
Sbjct: 5   MTFNSDLRITNSTVDIIPTTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFF 64

Query: 61  LLMSIIHLLFKPFGVPKISSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYM 120
           LLMSIIHLLFKPFGVPKISSQIIAGLVVGCSWGPLDDTKF LFRINSEEILGLLSYFGYM
Sbjct: 65  LLMSIIHLLFKPFGVPKISSQIIAGLVVGCSWGPLDDTKFRLFRINSEEILGLLSYFGYM 124

Query: 121 LFLFITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIV 180
           LFLFITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIV
Sbjct: 125 LFLFITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIV 184

Query: 181 SIQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLG 240
           SIQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLG
Sbjct: 185 SIQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLG 244

Query: 241 YFNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQP 300
           YFNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQP
Sbjct: 245 YFNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQP 304

Query: 301 AVFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAA 360
           AVFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAA
Sbjct: 305 AVFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAA 364

Query: 361 FARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKI 420
           FARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKI
Sbjct: 365 FARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKI 424

Query: 421 INHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKN 480
           INHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKN
Sbjct: 425 INHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKN 484

Query: 481 EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL 540
           EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL
Sbjct: 485 EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL 544

Query: 541 SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ 600
           SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ
Sbjct: 545 SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ 604

Query: 601 EDNMLRNLNCSVLQKADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAI 660
           EDNMLRNLNCSVLQKADCSVAIFADKGHLGS ASMASSGTQFRYSVCLIYLGGSDDREAI
Sbjct: 605 EDNMLRNLNCSVLQKADCSVAIFADKGHLGSXASMASSGTQFRYSVCLIYLGGSDDREAI 664

Query: 661 SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC 720
           SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC
Sbjct: 665 SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC 724

Query: 721 DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR 780
           DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR
Sbjct: 725 DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR 784

Query: 781 TSVLI 786
           TSVL+
Sbjct: 785 TSVLV 789

BLAST of MS000462 vs. ExPASy TrEMBL
Match: A0A5A7SGA0 (Cation/H(+) antiporter 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold2405G00070 PE=4 SV=1)

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 800/1559 (51.31%), Postives = 1039/1559 (66.65%), Query Frame = 0

Query: 19   TTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKI 78
            +TIC+ +PP V S GIW     SEWWL  SLPLLE QL      ++I +   K FG+ KI
Sbjct: 13   STICIDLPPYVNSKGIWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRFGISKI 72

Query: 79   SSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAAKMDVRMILKT 138
            S QI+ GL  G SW   D+ K     + S+E+L LL+  GY L+ F+ AAK+D+RM + T
Sbjct: 73   SCQILIGLAFGWSWNEWDEAKRKHLNVGSQEVLALLAELGYTLYTFLIAAKVDLRMTVAT 132

Query: 139  GRKAWIIGIPSTLIPIICGFWVGSYFLDG--LSPAEKNRVPVIVSIQSFISFPVIASLLS 198
            G+ A +IGI + L+P+I    V S  ++   L+  ++  +P + S  +  SFPV+ASL+ 
Sbjct: 133  GKSALLIGISALLLPLITETLVVSMVVEDWELTLRQRLALPALSSFHAKSSFPVVASLVK 192

Query: 199  ELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLIIF 258
            EL ++++ELGRLGLSSAL+ D+ G F++    QIR    N +        +M+   +  F
Sbjct: 193  ELHIMNSELGRLGLSSALVNDIFGTFILTMQVQIRRYYLNTSSVSTELGALMMLILVAFF 252

Query: 259  VLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGA 318
            VLRP++ WI+KQTP+G PV      GV  L  L  V   + G  ++ G Y+LGLAIP+GA
Sbjct: 253  VLRPTMFWIIKQTPQGMPVKSCYIDGVVSLAFLYIVLGNITGHASIIGAYVLGLAIPNGA 312

Query: 319  SGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVK 378
              L  +LV K+EC V + FMP+FV TC+L+AD+SK+        F ++NII+  V   VK
Sbjct: 313  P-LASTLVSKIECLVENVFMPIFVTTCALRADLSKISTTAFDVVFTKLNIILLCVACTVK 372

Query: 379  LVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTI 438
            LV     S YCKLP +D   LSLI+  KG VEL  Y V+   + I++ +   F++++L  
Sbjct: 373  LVASVSSSKYCKLPFKDALALSLIMCSKGPVELMAYTVSRDNRFIDNELFGCFVVWVLFF 432

Query: 439  ATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSE-LRILPCIHKNEHIYGLIHLLNLSCP 498
            AT VP+ VK L DP R ++ +QNRNIMHL+  S+ LR+L CIH++E++  +IHLLNLSCP
Sbjct: 433  ATIVPIAVKRLYDPSRKYAGYQNRNIMHLNRFSDKLRLLACIHQHENVNAIIHLLNLSCP 492

Query: 499  TPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVY 558
            T +N + V+  HLIEL G+ TPIFISH+++      + YS+ +  SFD FE++N  G+ Y
Sbjct: 493  TIENSIVVHIFHLIELPGQITPIFISHKRQGNPFDKRSYSQQIIRSFDKFERENE-GTAY 552

Query: 559  AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 618
             ECFT ++P   M ND+C LALDK+ S IILPFH TWT DG I++ D  +R LN S+L++
Sbjct: 553  VECFTSVSPCTKMHNDVCTLALDKIASFIILPFHITWTVDGSIERVDKNVRTLNYSILER 612

Query: 619  ADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 678
            A CSV IFA +  L    +   S     YSVC+I+LGG DDREA+S+AKRM  +  +ELT
Sbjct: 613  APCSVGIFAHRRKLEHFKARKRSS----YSVCVIFLGGKDDREALSYAKRMVNDLRVELT 672

Query: 679  VLKLGSPMEDGSSSK----WENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLR 738
            VL+L +  +  + SK    WE+++D EV+KDFK  CLG+  V Y EEVC DG +TAL LR
Sbjct: 673  VLRLQASKDYQNRSKSLNSWEHVMDEEVVKDFKGKCLGDERVVYEEEVCTDGQKTALILR 732

Query: 739  KIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTS--------- 798
            K+V+ FDLMIVGRR+GLE+PQT GL EWNEFPELG LGDLIAS D NT TS         
Sbjct: 733  KVVDMFDLMIVGRRNGLETPQTDGLDEWNEFPELGHLGDLIASSDINTGTSLLETQLNPN 792

Query: 799  ------------------------------------------------------------ 858
                                                                        
Sbjct: 793  MTSTICIDIPTYVNSKGLWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRLGIS 852

Query: 859  -----VLIGLIFGCSWRQFDKETINLFYERNQDILGLLAEFGYTLYLFLSGVKIDLSMTI 918
                 +L GL FG SW ++D+         +Q++L LLAE GYTLY FL   K+DL MT+
Sbjct: 853  KISCQILTGLAFGWSWNEWDEAKRKNLNIGSQEVLALLAELGYTLYTFLIAAKVDLRMTV 912

Query: 919  RAGKNALIIGILALLVPLFAANFVRSLLVDGSGISEADLSTLPILISFHSMSSFPVIASL 978
              GK AL+IGI ALL+PL     V S++V+   ++      LP L SFH++ SFPV+ASL
Sbjct: 913  ATGKRALLIGISALLLPLITETLVVSMVVEDLALTLRQRFALPSLSSFHAIISFPVVASL 972

Query: 979  LSDLEIVNSELGRLGLSSALFSDLLSLFMMVTARQAKRFKDVPSIASLQLGALILLFLLI 1038
            + +L I+NSELGRL LSSAL SD +  F+++     KR+    S  S ++GAL++L L+ 
Sbjct: 973  VKELHIMNSELGRLCLSSALISDFVGTFILIMKGHIKRYHMNTSRISTEVGALMVLILVA 1032

Query: 1039 LFVFRPAMLWIIRQTPEGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPD 1098
             FV RPAM WII+QTP+GMPVK  YI  V+FL L   VL  FTG  SI+G YV GLA+PD
Sbjct: 1033 FFVLRPAMFWIIKQTPQGMPVKSCYIDGVVFLALLYIVLGTFTGHASIIGAYVMGLAIPD 1092

Query: 1099 GAPLASTLVNNIECLVSDVFMPILVITCALKADLSQIS-SSFDAAFTKLNIVLILVTFVV 1158
            GAPLASTLV+  ECLV DVFMPI V TCAL+ADLS+IS ++FD  FTKLNI+L+ V   V
Sbjct: 1093 GAPLASTLVSKFECLVEDVFMPIFVTTCALRADLSKISATTFDVVFTKLNIILLCVACTV 1152

Query: 1159 KIVSCFLTSRHCRLPFRDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVF 1218
            K V+   +SR+C+LPF+D+LALSLIM +KG VEL+ YT+ RD   I++ L G   + I+F
Sbjct: 1153 KFVASVSSSRYCKLPFKDALALSLIMCSKGPVELIFYTIFRDNRFIDNELFGCFVVWILF 1212

Query: 1219 VATLVPILVKCLYDPSMKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSC 1278
             AT+VPI VK LYDPS KYA YQ+RNIMHLN  SDKLRLLACIHQ+EN+N+IIHLLNLSC
Sbjct: 1213 FATMVPIAVKGLYDPSRKYACYQDRNIMHLNRFSDKLRLLACIHQHENVNAIIHLLNLSC 1272

Query: 1279 PTAENPLAIHILHLIELSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTV 1338
            PT EN + +HI HLIEL GR  P+FISHK   N + + RSYSQ I+HSFD+FER+++GT 
Sbjct: 1273 PTIENSIIVHIFHLIELPGRITPIFISHKRQGN-SFDKRSYSQQIVHSFDKFERENEGTA 1332

Query: 1339 CVECFTAISPNRLMHNDICTLALDKITSLIIMPFHRTWTVDGFAYHDDNMVRTLNCSVLG 1398
            CVEC+T++SP  +MHND+CTLALDKI S II+PFH TWTVDG     D  VRTLN S+L 
Sbjct: 1333 CVECYTSVSPCTVMHNDVCTLALDKIASFIILPFHITWTVDGSIGRVDKNVRTLNYSILE 1392

Query: 1399 RAPCSIGIFADRGRLGSIAATAIPRSRYSVCVLFLGGKDDREAISYAKRMAKDSRVELTM 1458
            RAPCS+GIFA R +L    A    RS YSVCV+FLGGKDDREA+SYAKRM KD RVELT+
Sbjct: 1393 RAPCSVGIFAHRSKLEHFRAR--KRSSYSVCVIFLGGKDDREALSYAKRMVKDLRVELTV 1452

Query: 1459 LRLRGPP--EEHGQKTDNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRK 1494
            LRL+ P   +   +  ++WE I+D EV++DF+ KCLGD RVVY+E +C DGQ+TAF++RK
Sbjct: 1453 LRLKAPQNYQNRSKLHNSWEYIMDEEVVKDFKGKCLGDERVVYEEKICGDGQETAFLLRK 1512

BLAST of MS000462 vs. ExPASy TrEMBL
Match: A0A5D3E7Y0 (Cation/H(+) antiporter 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold788G00110 PE=4 SV=1)

HSP 1 Score: 1476.1 bits (3820), Expect = 0.0e+00
Identity = 795/1522 (52.23%), Postives = 1032/1522 (67.81%), Query Frame = 0

Query: 19   TTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKI 78
            +TIC+ +PP V S GIW     SEWWL  SLPLLE QL      ++I +   K FG+ KI
Sbjct: 13   STICIDLPPYVNSKGIWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRFGISKI 72

Query: 79   SSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAAKMDVRMILKT 138
            S QI+ GL  G SW   D+ K     + S+E+L LL+  GY  + F+ AAK+D+RM + T
Sbjct: 73   SCQILIGLAFGWSWNEWDEAKRKHLNVGSQEVLALLAELGYTFYTFLIAAKVDLRMTVAT 132

Query: 139  GRKAWIIGIPSTLIPIICGFWVGSYFLDG--LSPAEKNRVPVIVSIQSFISFPVIASLLS 198
            G+ A +IGI + L+P+I    V S  ++   L+  ++  +P + S  +  SFPV+ASL+ 
Sbjct: 133  GKSALLIGISALLLPLITETLVVSMVVEDWELTLRQRLALPALSSFHAKSSFPVVASLVK 192

Query: 199  ELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLIIF 258
            EL ++++ELGRLGLSSALI D+ G F+     QIR    N +        +M+   +  F
Sbjct: 193  ELHIMNSELGRLGLSSALINDIFGTFISTMQVQIRRYYLNTSSVSTELGALMMLILVAFF 252

Query: 259  VLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGA 318
            VLRP++ WI+KQTP+G PV      GV  L  L  V   L G  ++ G Y+LGLA PDGA
Sbjct: 253  VLRPTMFWIIKQTPQGMPVKSCYIDGVVSLAFLYIVLGNLTGHASIIGAYVLGLANPDGA 312

Query: 319  SGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVK 378
              L  +LV K+E  V + FMP+FV TC+L+AD+SK+        F ++NII+  V   VK
Sbjct: 313  P-LASTLVSKIESLVENVFMPIFVTTCALRADLSKISTTAFDVVFTKLNIILLCVACTVK 372

Query: 379  LVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTI 438
            LV     S YCKLP +D   LSLI+  KG VEL  Y V+   + I++ +   F++++L  
Sbjct: 373  LVASVSSSKYCKLPFKDALALSLIMCSKGPVELMAYTVSRDNRFIDNELFGCFVVWILFF 432

Query: 439  ATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSE-LRILPCIHKNEHIYGLIHLLNLSCP 498
            AT VP+ V  L DP R ++ +QNRNIMHL+  S+ LR+L CIH++E++  +IHLLNLSCP
Sbjct: 433  ATIVPIAVIRLYDPSRKYAGYQNRNIMHLNRFSDKLRLLACIHQHENVNAIIHLLNLSCP 492

Query: 499  TPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVY 558
            T +N + V+  HLIEL G+ TPIFISH+++      + YS+ +  SFD FE++N  G+ Y
Sbjct: 493  TIENSIVVHIFHLIELPGQITPIFISHKRQGNPFDKRSYSQQIIRSFDKFERENE-GTAY 552

Query: 559  AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 618
             ECFT ++P   M ND+C LALDK+ S IILPFH TWT DG I++ D  +R LN S+L++
Sbjct: 553  VECFTSVSPCTKMHNDVCTLALDKIASFIILPFHITWTMDGSIERVDKNVRTLNYSILER 612

Query: 619  ADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 678
            A CSV IFA +  L    +   S     YSVC+I+LGG DDREA+S+AKRM  +  +ELT
Sbjct: 613  APCSVGIFAHRRKLEHFKARKRSS----YSVCVIFLGGKDDREALSYAKRMVNDLRVELT 672

Query: 679  VLKLGSPMEDGSSSK----WENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLR 738
            VL+L +  +  + SK    WE+++D EV+KDFK  CLG+  V Y EEVC DG +TAL LR
Sbjct: 673  VLRLQTSKDYQNRSKSLNSWEHVMDEEVVKDFKEKCLGDERVVYEEEVCTDGQKTALILR 732

Query: 739  KIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTS--------- 798
            K+V+ FDLMIVGRR+GLE+PQT GL EWNEFPELG LGDLIAS D NT TS         
Sbjct: 733  KVVDMFDLMIVGRRNGLETPQTDGLDEWNEFPELGHLGDLIASSDINTGTSLLPSLPLLE 792

Query: 799  ----------------------------VLIGLIFGCSWRQFDKETINLFYERNQDILGL 858
                                        +L GL FG SW ++D+         +Q++L L
Sbjct: 793  FQLIVLCFSLAITYFFLKRLGISKISCQILTGLAFGWSWNEWDEAKRKNLNIGSQEVLAL 852

Query: 859  LAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDGSGISEA 918
            LAE GYTLY FL   K+DL MT+  GK AL+IGI ALL+PL     V S++V+   ++  
Sbjct: 853  LAELGYTLYTFLIAAKVDLRMTVATGKRALLIGISALLLPLITETLVVSMVVEDLALTLR 912

Query: 919  DLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFMMVTARQAK 978
                LP L SFH++ SFPV+ASL+ +L I+NSELGRL LSSAL SD +  F+++     K
Sbjct: 913  QRFALPSLSSFHAIISFPVVASLVKELHIMNSELGRLCLSSALISDFVGTFILIMKGHIK 972

Query: 979  RFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQAVIFLVLFTT 1038
            R+    S  S ++GAL++L L+  FV RP M WII+QTP+GMPVK  YI  V+FL L   
Sbjct: 973  RYHMNTSRISTEVGALMVLILVAFFVLRPTMFWIIKQTPQGMPVKSCYIDGVVFLALLYI 1032

Query: 1039 VLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITCALKADLSQI 1098
            VL  FTG  SI+G YV GLA+PDGAPLASTLV+  ECLV DVFMPI V TCAL+ADLS+I
Sbjct: 1033 VLGTFTGHASIIGAYVMGLAIPDGAPLASTLVSKFECLVEDVFMPIFVTTCALRADLSKI 1092

Query: 1099 S-SSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNKGAVELVLY 1158
            S ++FD  FTKLNI+L+ V   VK V+   +SR+C+LPF+D+LALSLIM +KG VEL+ Y
Sbjct: 1093 SATTFDVVFTKLNIILLCVACTVKFVASVSSSRYCKLPFKDALALSLIMCSKGPVELIFY 1152

Query: 1159 TMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMHLNPASDKL 1218
            T+ RD   I++ L G   + I+F AT+VPI VK LYDPS KYA YQ+RNIMHLN  SDKL
Sbjct: 1153 TIFRDNRFIDNELFGCFVVWILFFATMVPIAVKGLYDPSRKYACYQDRNIMHLNRFSDKL 1212

Query: 1219 RLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHLIELSGRTAPVFISHKYFHNKALN 1278
            RLLACIHQ+EN+N+IIHLLNLSCPT EN + +HI HLIEL GR  P+FISHK   N + +
Sbjct: 1213 RLLACIHQHENVNAIIHLLNLSCPTIENSIIVHIFHLIELPGRITPIFISHKRQGN-SFD 1272

Query: 1279 DRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITSLIIMPFHRT 1338
             RSYSQ I+HSFD+FE++++GT CVEC+T++SP  +MHND+CTLALDKI S II+PFH T
Sbjct: 1273 KRSYSQQIVHSFDKFEKENEGTACVECYTSVSPCTVMHNDVCTLALDKIASFIILPFHIT 1332

Query: 1339 WTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIAATAIPRSRYSVCVLFLGG 1398
            WTVDG     D  VRTLN S+L +APCS+GIF  R +L    A    RS YSVCV+FLGG
Sbjct: 1333 WTVDGSIGRVDKNVRTLNYSILEKAPCSVGIFVHRSKLEHFRAR--KRSSYSVCVIFLGG 1392

Query: 1399 KDDREAISYAKRMAKDSRVELTMLRLRGPP--EEHGQKTDNWEDILDAEVLRDFRSKCLG 1458
            KDDREA+SYAKRM KD RVELT+LRL+ P   +   +  ++WE I+D EV++DF+ KCLG
Sbjct: 1393 KDDREALSYAKRMVKDLRVELTVLRLKAPKNYQNRSKLHNSWEYIMDEEVVKDFKGKCLG 1452

Query: 1459 DGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRRNGLKSSQTDGLNEWNEFPELGVLG 1494
            D RVVY+E +C DGQ+TAF++RK+V++FDLMIVGRRNGL++ QTDGLNEWNEFPELG LG
Sbjct: 1453 DERVVYEEKICGDGQETAFLLRKVVDMFDLMIVGRRNGLETPQTDGLNEWNEFPELGYLG 1512

BLAST of MS000462 vs. ExPASy TrEMBL
Match: A0A1S3AYQ2 (uncharacterized protein LOC103484221 OS=Cucumis melo OX=3656 GN=LOC103484221 PE=4 SV=1)

HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 794/1574 (50.44%), Postives = 1035/1574 (65.76%), Query Frame = 0

Query: 19   TTICLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGV--- 78
            +TIC+ +PP V S GIW     SEWWL  SLPLLE QL      ++I +   K F +   
Sbjct: 13   STICIDLPPYVNSKGIWVEFDDSEWWLKPSLPLLEFQLIVLCFSLAITYFFLKRFAIKPD 72

Query: 79   ------------PKISSQIIAGLVVGCSWGPLDDTKFILFRINSEEILGLLSYFGYMLFL 138
                          ++  +  GL  G SW   D+ K     + S+E+L LL+  GY L+ 
Sbjct: 73   GKIQKNLNTIYSKNLNPVLRIGLAFGWSWNEWDEAKRKHLNVGSQEVLALLAELGYTLYT 132

Query: 139  FITAAKMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDG--LSPAEKNRVPVIVS 198
            F+ AAK+D+RM + TG+ A +IGI + L+P+I    V S  ++   L+  ++  +P + S
Sbjct: 133  FLIAAKVDLRMTVATGKSALLIGISALLLPLITETLVVSMVVEDWELTLRQRLALPALSS 192

Query: 199  IQSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGY 258
              +  SFPV+ASL+ EL ++++ELGRLGLSSAL+ D+ G F++    QIR    N +   
Sbjct: 193  FHAKSSFPVVASLVKELHIMNSELGRLGLSSALVNDIFGTFILTMQVQIRRYYLNTSSVS 252

Query: 259  FNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPA 318
                 +M+   +  FVLRP++ WI+KQTP+G PV      GV  L  L  V   + G  +
Sbjct: 253  TELGALMMLILVAFFVLRPTMFWIIKQTPQGMPVKSCYIDGVVSLAFLYIVLGNITGHAS 312

Query: 319  VFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAF 378
            + G Y+LGLAIP+GA  L  +LV K+EC V + FMP+FV TC+L+AD+SK+        F
Sbjct: 313  IIGAYVLGLAIPNGAP-LASTLVSKIECLVENVFMPIFVTTCALRADLSKISTTAFDVVF 372

Query: 379  ARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKII 438
             ++NII+  V   VKLV     S YCKLP +D   LSLI+  KG VEL  Y V+   + I
Sbjct: 373  TKLNIILLCVACTVKLVASVSSSKYCKLPFKDALALSLIMCSKGPVELMAYTVSRDNRFI 432

Query: 439  NHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSE-LRILPCIHKN 498
            ++ +   F++++L  AT VP+ VK L DP R ++ +QNRNIMHL+  S+ LR+L CIH++
Sbjct: 433  DNELFGCFVVWVLFFATIVPIAVKRLYDPSRKYAGYQNRNIMHLNRFSDKLRLLACIHQH 492

Query: 499  EHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTL 558
            E++  +IHLLNLSCPT +N + V+  HLIEL G+ TPIFISH+++      + YS+ +  
Sbjct: 493  ENVNAIIHLLNLSCPTIENSIVVHIFHLIELPGQITPIFISHKRQGNPFDKRSYSQQIIR 552

Query: 559  SFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQ 618
            SFD FE++N  G+ Y ECFT ++P   M ND+C LALDK+ S IILPFH TWT DG I++
Sbjct: 553  SFDKFERENE-GTAYVECFTSVSPCTKMHNDVCTLALDKIASFIILPFHITWTVDGSIER 612

Query: 619  EDNMLRNLNCSVLQKADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAI 678
             D  +R LN S+L++A CSV IFA +  L    +   S     YSVC+I+LGG DDREA+
Sbjct: 613  VDKNVRTLNYSILERAPCSVGIFAHRRKLEHFKARKRSS----YSVCVIFLGGKDDREAL 672

Query: 679  SFAKRMAKEPTIELTVLKLGSPMEDGSSSK----WENMLDSEVLKDFKMNCLGNGGVRYF 738
            S+AKRM  +  +ELTVL+L +  +  + SK    WE+++D EV+KDFK  CLG+  V Y 
Sbjct: 673  SYAKRMVNDLRVELTVLRLQASKDYQNRSKSLNSWEHVMDEEVVKDFKGKCLGDERVVYE 732

Query: 739  EEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLD 798
            EEVC DG +TAL LRK+V+ FDLMIVGRR+GLE+PQT GL EWNEFPELG LGDLIAS D
Sbjct: 733  EEVCTDGQKTALILRKVVDMFDLMIVGRRNGLETPQTDGLDEWNEFPELGHLGDLIASSD 792

Query: 799  NNTRTS------------------------------------------------------ 858
             NT TS                                                      
Sbjct: 793  INTGTSLLETQLNPNMTSTICIDIPTYVNSKGLWVEFDDSEWWLKPSLPLLEFQLIVLCF 852

Query: 859  --------------------VLIGLIFGCSWRQFDKETINLFYERNQDILGLLAEFGYTL 918
                                +L GL FG SW ++D+         +Q++L LLAE GYTL
Sbjct: 853  SLAITYFFLKRLGISKISCQILTGLAFGWSWNEWDEAKRKNLNIGSQEVLALLAELGYTL 912

Query: 919  YLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDGSGISEADLSTLPIL 978
            Y FL   K+DL MT+  GK AL+IGI ALL+PL     V S++V+   ++      LP L
Sbjct: 913  YTFLIAAKVDLRMTVATGKRALLIGISALLLPLITETLVVSMVVEDLALTLRQRFALPSL 972

Query: 979  ISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFMMVTARQAKRFKDVPSI 1038
             SFH++ SFPV+ASL+ +L I+NSELGRL LSSAL SD +  F+++     KR+    S 
Sbjct: 973  SSFHAIISFPVVASLVKELHIMNSELGRLCLSSALISDFVGTFILIMKGHIKRYHMNTSR 1032

Query: 1039 ASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQAVIFLVLFTTVLFNFTGQ 1098
             S ++GAL++L L+  FV RPAM WII+QTP+GMPVK  YI  V+FL L   VL  FTG 
Sbjct: 1033 ISTEVGALMVLILVAFFVLRPAMFWIIKQTPQGMPVKSCYIDGVVFLALLYIVLGTFTGH 1092

Query: 1099 ISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITCALKADLSQIS-SSFDAA 1158
             SI+G YV GLA+PDGAPLASTLV+  ECLV DVFMPI V TCAL+ADLS+IS ++FD  
Sbjct: 1093 ASIIGAYVMGLAIPDGAPLASTLVSKFECLVEDVFMPIFVTTCALRADLSKISATTFDVV 1152

Query: 1159 FTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNKGAVELVLYTMARDYNA 1218
            FTKLNI+L+ V   VK V+   +SR+C+LPF+D+LALSLIM +KG VEL+ YT+ RD   
Sbjct: 1153 FTKLNIILLCVACTVKFVASVSSSRYCKLPFKDALALSLIMCSKGPVELIFYTIFRDNRF 1212

Query: 1219 INDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMHLNPASDKLRLLACIHQ 1278
            I++ L G   + I+F AT+VPI VK LYDPS KYA YQ+RNIMHLN  SDKLRLLACIHQ
Sbjct: 1213 IDNELFGCFVVWILFFATMVPIAVKGLYDPSRKYACYQDRNIMHLNRFSDKLRLLACIHQ 1272

Query: 1279 NENINSIIHLLNLSCPTAENPLAIHILHLIELSGRTAPVFISHKYFHNKALNDRSYSQHI 1338
            +EN+N+IIHLLNLSCPT EN + +HI HLIEL GR  P+FISHK   N + + RSYSQ I
Sbjct: 1273 HENVNAIIHLLNLSCPTIENSIIVHIFHLIELPGRITPIFISHKRQGN-SFDKRSYSQQI 1332

Query: 1339 IHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITSLIIMPFHRTWTVDGFAY 1398
            +HSFD+FER+++GT CVEC+T++SP  +MHND+CTLALDKI S II+PFH TWTVDG   
Sbjct: 1333 VHSFDKFERENEGTACVECYTSVSPCTVMHNDVCTLALDKIASFIILPFHITWTVDGSIG 1392

Query: 1399 HDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIAATAIPRSRYSVCVLFLGGKDDREAIS 1458
              D  VRTLN S+L RAPCS+GIFA R +L    A    RS YSVCV+FLGGKDDREA+S
Sbjct: 1393 RVDKNVRTLNYSILERAPCSVGIFAHRSKLEHFRAR--KRSSYSVCVIFLGGKDDREALS 1452

Query: 1459 YAKRMAKDSRVELTMLRLRGPP--EEHGQKTDNWEDILDAEVLRDFRSKCLGDGRVVYKE 1494
            YAKRM KD RVELT+LRL+ P   +   +  ++WE I+D EV++DF+ KCLGD RVVY+E
Sbjct: 1453 YAKRMVKDLRVELTVLRLKAPQNYQNRSKLHNSWEYIMDEEVVKDFKGKCLGDERVVYEE 1512

BLAST of MS000462 vs. ExPASy TrEMBL
Match: A0A6J1CI53 (cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111011605 PE=4 SV=1)

HSP 1 Score: 1176.0 bits (3041), Expect = 0.0e+00
Identity = 583/656 (88.87%), Postives = 620/656 (94.51%), Query Frame = 0

Query: 130 MDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIVSIQSFISFP 189
           MDVR  +KTGRKAWIIGIPS  +P+ICG +VGSYF DGLS AEKN VP++VSI SFISFP
Sbjct: 1   MDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFP 60

Query: 190 VIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMV 249
           VIASLLSELK+VSTELGRLG+SSALIADL  QFVM TANQIRISR++ITLGYF+KAGIMV
Sbjct: 61  VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMV 120

Query: 250 QFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLG 309
           Q CLI+FVLRPSVLW+LKQTPEGKPVSR TTHG+F+ VL+SAV+SALLGQPA+FGPYLLG
Sbjct: 121 QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG 180

Query: 310 LAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIIT 369
           LAIPDGA+ LGFSLVEK+ECFVSDFF+PLFVITCSLQ D+SK+F+V  AA  AR+NI+++
Sbjct: 181 LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLS 240

Query: 370 LVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWF 429
           LVTYGVKLVCCFLCSL CKLPLRD FVLSLILSCKGV EL F  +  QY++IN GILAWF
Sbjct: 241 LVTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWF 300

Query: 430 ILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNEHIYGLIHL 489
           I+FLL +ATFVPLTVKWLTDPKRTH  +QNRNIMHLSPNSELR+LPCIHKNEHIYGLIHL
Sbjct: 301 IVFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHL 360

Query: 490 LNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDN 549
           LNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQEN AI+DQ YSENVTLSFDHFEKDN
Sbjct: 361 LNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDN 420

Query: 550 SAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLN 609
           SAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLN
Sbjct: 421 SAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLN 480

Query: 610 CSVLQKADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKE 669
           CSVLQKADCSVAIFADKGHLGS ASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKE
Sbjct: 481 CSVLQKADCSVAIFADKGHLGSXASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKE 540

Query: 670 PTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALT 729
           PTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALT
Sbjct: 541 PTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALT 600

Query: 730 LRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLI 786
           LRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVL+
Sbjct: 601 LRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLV 655

BLAST of MS000462 vs. TAIR 10
Match: AT3G44900.1 (cation/H+ exchanger 4 )

HSP 1 Score: 500.7 bits (1288), Expect = 3.9e-141
Identity = 290/708 (40.96%), Postives = 429/708 (60.59%), Query Frame = 0

Query: 809  QDILGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDG 868
            + + GL+    Y ++ FL GVK+DLS+    G+ A+ IG+ ++L+ +     +  L++  
Sbjct: 115  ETLFGLVGACSYMMFWFLMGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRD 174

Query: 869  SGISEAD--LSTLPILISF--HSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSL 928
             G  + +  +S   I+  +    +SSFPVI +LL +L + NSELGRL +SSA+ SD  + 
Sbjct: 175  VGTKKGEPVMSFFEIIFIYLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTS 234

Query: 929  FMMVTARQAKRFKD--------------VPSIASLQLGALILLFLLILFVFRPAMLWIIR 988
             +       K  KD              V +    + G ++L     +++FRP M +II+
Sbjct: 235  ILSAVLVFLKELKDDKSRLGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIK 294

Query: 989  QTPEGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIE 1048
            +TP G PVK  YI A+I LV  + +L ++  Q   +GP++ GLAVP G PL S ++   E
Sbjct: 295  RTPSGRPVKKFYIYAIIILVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFE 354

Query: 1049 CLVSDVFMPILVITCALKADLSQISSSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRL 1108
             +V   F+P  V T A + D S + S  D    K  ++L+ V+F+VK     L +    +
Sbjct: 355  SVVFGTFLPFFVATSAEEIDTSILQSWID---LKSIVILVSVSFIVKFALTTLPAFLYGM 414

Query: 1109 PFRDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYD 1168
            P +D +ALSLIMS KG  E   Y  A     I         L I+  + ++P L+K +YD
Sbjct: 415  PAKDCIALSLIMSFKGIFEFGAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYD 474

Query: 1169 PSMKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHL 1228
            PS  YAGY+ RN++H+ P S+ LR+L+CI++ ++I  +I+LL  +CP+ ENP+A ++LHL
Sbjct: 475  PSRMYAGYEKRNMLHMKPNSE-LRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHL 534

Query: 1229 IELSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLM 1288
            +EL G+  PV ISH+    K+ N    S++++ SF+QF  D  G+V V  +TA+S  ++M
Sbjct: 535  MELVGQANPVLISHRLQTRKSENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMM 594

Query: 1289 HNDICTLALDKITSLIIMPFHRTWTVDGFAYHDDN-MVRTLNCSVLGRAPCSIGIFADRG 1348
            H DIC LAL+  TSLII+PFH+TW+ DG A   D+ M+R LN SVL  +PCS+GIF  R 
Sbjct: 595  HGDICMLALNNTTSLIILPFHQTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRS 654

Query: 1349 RLG--SIAATAIPRSRYSVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPEEHG 1408
              G  +I  TA   S Y VC+LFLGGKDDREA+S AKRMA+DSR+ +T++ L    +   
Sbjct: 655  SNGRRTIKETAANFSSYQVCMLFLGGKDDREALSLAKRMARDSRITITVVSLISSEQRAN 714

Query: 1409 QKTDNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRR 1468
            Q TD W+ +LD E+LRD +S  L    +V+ E+V  D  QT+ +++ I N +DL IVGR 
Sbjct: 715  QATD-WDRMLDLELLRDVKSNVLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGRE 774

Query: 1469 NGLKSSQTDGLNEWNEFPELGVLGDLIASTDINSRASLLVIQQQQIVI 1496
             G KS  T+GL EW+EF ELG++GDL+ S D+N +AS+LVIQQQQ +I
Sbjct: 775  KGRKSVFTEGLEEWSEFEELGIIGDLLTSQDLNCQASVLVIQQQQQMI 817

BLAST of MS000462 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 476.9 bits (1226), Expect = 6.1e-134
Identity = 280/715 (39.16%), Postives = 429/715 (60.00%), Query Frame = 0

Query: 811  ILGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAAN--FVRSLLVDG 870
            +  L A   Y ++ FL GVK+D  +    G+ A+ IG+ ++L+     +  F  +L   G
Sbjct: 118  VFSLTAACSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVG 177

Query: 871  SGISEADLSTLP--ILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFM 930
            +  S+  L++L   ++ S   +SSFPV+ +LL +L + NSELGRL +SSA+ SD  +  +
Sbjct: 178  TKNSDHTLNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSIL 237

Query: 931  MVTARQAKRFKDVPS----------IAS----LQLGALILLFLLILFVFRPAMLWIIRQT 990
                   K  KD  +          IA     ++ G ++L   + ++VFRP M +II+QT
Sbjct: 238  ASVLIFMKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQT 297

Query: 991  PEGMPVKGSYIQAVIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECL 1050
            P G PVK  Y+  +I +V  + +L N+  Q   MGP++ GLAVP G PL S ++   E  
Sbjct: 298  PSGRPVKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESA 357

Query: 1051 VSDVFMPILVITCALKADLSQISSSFDAAFTKLN-IVLILVT-FVVKIVSCFLTSRHCRL 1110
            +   F+P  + + + + D+S +       +  LN I+LI+VT FVVK +   + +    +
Sbjct: 358  IFGTFLPFFIASSSTEIDISAL-----FGWEGLNGIILIMVTSFVVKFIFTTVPALFYGM 417

Query: 1111 PFRDSLALSLIMSNKGAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYD 1170
            P  D  ALSLIMS KG  EL  Y +A    ++         L I   + ++P +++ LYD
Sbjct: 418  PMEDCFALSLIMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYD 477

Query: 1171 PSMKYAGYQNRNIMHLNPASDKLRLLACIHQNENINSIIHLLNLSCPTAENPLAIHILHL 1230
            PS  YAGY+ RN+ HL P S+ LR+L+CI++ ++I+ +I+LL   CP+ E+P+A ++LHL
Sbjct: 478  PSRMYAGYEKRNMQHLKPNSE-LRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHL 537

Query: 1231 IELSGRTAPVFISHKYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLM 1290
            +EL G+  P+FISHK    +   + SYS +++ SF++F +D  G+V V  +TA+S    M
Sbjct: 538  MELVGQANPIFISHK-LQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTM 597

Query: 1291 HNDICTLALDKITSLIIMPFHRTWTVDGFA-YHDDNMVRTLNCSVLGRAPCSIGIFADRG 1350
            H DIC LAL+  TSLI++PFH+TW+ DG A   ++NM+R LN SVL  APCS+G+F  R 
Sbjct: 598  HGDICMLALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRS 657

Query: 1351 RLG---------SIAATAIPRSRYSVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLR 1410
              G         +I  T    S Y++C++FLGGKDDREA++ A RMA+D R+ +T++RL 
Sbjct: 658  SSGRKNISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLI 717

Query: 1411 GPPEEHGQKTDNWEDILDAEVLRDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFD 1470
               +E  ++   W+ +LD E+LRD +S  L D  + Y E    D  +T+ ++R +V+ FD
Sbjct: 718  -TTDEKARENTVWDKMLDDELLRDVKSNTLVD--IFYSEKAIEDAAETSSLLRSMVSDFD 777

Query: 1471 LMIVGRRNGLKSSQTDGLNEWNEFPELGVLGDLIASTDINSRASLLVIQQQQIVI 1496
            + IVGR NG  S  T+GL EW+EF ELG++GDL+ S D N +AS+LVIQQQQ++I
Sbjct: 778  MFIVGRGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQQQLMI 822

BLAST of MS000462 vs. TAIR 10
Match: AT3G44920.1 (cation/H+ exchanger 11 )

HSP 1 Score: 436.8 bits (1122), Expect = 7.0e-122
Identity = 266/687 (38.72%), Postives = 397/687 (57.79%), Query Frame = 0

Query: 812  LGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLL--VDGS 871
            L  ++ FG  ++ FL  V+    +   +GK  ++IGI++   PLF+ +F+      +D  
Sbjct: 103  LRCISVFGRLMFTFLMTVRTSRRVAFHSGKLPVVIGIVSFFAPLFSLSFLNLFTDNIDPH 162

Query: 872  GIS-EADLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFMMV 931
             +S +  L+   +++   S    P    +L +L+I+NSELGRL LS++  +D+L +F M+
Sbjct: 163  YMSLDKALAERTVIVITQSQILLPSTTYILLELKIINSELGRLALSASAINDMLGIFAMI 222

Query: 932  TA-RQAKRFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQAVI 991
             A  QA       +IA   L A+I+ FL++ FVF+P + WII +TPE  PV+  YI AVI
Sbjct: 223  VATTQATYIHVSHAIAYRDLVAVIIFFLIVFFVFKPMVQWIIDRTPEDKPVEDIYIHAVI 282

Query: 992  FLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITCAL 1051
                 +   F F     ++GP + G+ +P+G PL S L    E L  +VF+PI +   A+
Sbjct: 283  LTAFASAAYFVFFNMKYVLGPLIIGIIIPEGPPLGSALEAKFERLTMNVFLPISITFSAM 342

Query: 1052 KADLSQISSSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNKGA 1111
            + D  +I S F   +   NI L L+  V+K+V+C     + +LP  +SLA+SLI+S K  
Sbjct: 343  RCDGLRILSQFTDIY--FNIFLTLLILVIKLVACLTLCLYYKLPRSESLAVSLILSYKSF 402

Query: 1112 VELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMHLN 1171
            VE VLY    +   I+     +  L  +  A +VP++V+ +YDP  KY  YQ R+I+HL 
Sbjct: 403  VEFVLYEAVLEEKFISQATYAFLILYSLLSAGIVPMVVRSMYDPKRKYVNYQKRDILHLE 462

Query: 1172 PASDKLRLLACIHQNENINSIIHLLNL-SCPTAENPLAIHILHLIELSGRTAPVFISHKY 1231
             A+  LR+L C+H+ EN++  I  L L S P  + P+A+ +LHL++L G+  P+ +SH  
Sbjct: 463  -ANSGLRILTCLHKPENVSETIAFLQLFSSPIHDFPIAVTVLHLVKLVGQINPIIVSHDK 522

Query: 1232 FHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITSLI 1291
               K L+  SY      +F QF ++S  +V V  FTA S   LMH DICTLALD+ TS+I
Sbjct: 523  -KLKRLHKNSYIHTANLAFRQFMQESLESVTVTTFTAFSHENLMHEDICTLALDRTTSMI 582

Query: 1292 IMPFHRTWTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIA-ATAIPRSRYS 1351
            ++P  R WTVDG    DD   R LN S+L RAPCSIGI  DRG+    +  T+  R    
Sbjct: 583  VVPSGRKWTVDGMFESDDLAARQLNQSLLDRAPCSIGILVDRGQFSRKSYVTSKNRYNID 642

Query: 1352 VCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPEEHGQKTDNWEDILDAEVLRDF 1411
            V VLF+GGKDDREA+S  KRM  + RV +T++RL    E   +    W+ ILD E L+D 
Sbjct: 643  VGVLFIGGKDDREALSLVKRMKYNPRVRVTVIRLIFDHEIESE----WDYILDNEGLKDL 702

Query: 1412 RSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRRNGLKSSQTDGLNEWNEFP 1471
            +S    +  ++Y E +     +    ++ +   +DLM+VGR + + S    GL EW E P
Sbjct: 703  KSTESNED-ILYTERIVTSVVEVVKAVQLLAEEYDLMVVGRDHDMTSQDLSGLTEWVELP 762

Query: 1472 ELGVLGDLIASTDINSRASLLVIQQQQ 1493
            ELGV+GDL+A+ D+NS+ S+LV+QQQQ
Sbjct: 763  ELGVIGDLLAARDLNSKVSVLVVQQQQ 780

BLAST of MS000462 vs. TAIR 10
Match: AT3G44910.1 (cation/H+ exchanger 12 )

HSP 1 Score: 428.3 bits (1100), Expect = 2.5e-119
Identity = 267/785 (34.01%), Postives = 414/785 (52.74%), Query Frame = 0

Query: 22  CLLMPPRVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKISSQ 81
           C+ +   + S G W N  + +     SLPL+E Q+   F+ + IIH   K FG+  I S 
Sbjct: 10  CIPLVFNISSFGFWENLKSPDVIFGYSLPLMEFQILLIFVFIIIIHSFLKSFGISPIPSY 69

Query: 82  IIAGLVVG-------------CSWGPLDDTKFILFRINSEEILGLLSYFGYMLFLFITAA 141
           ++AGL++G              SW P          ++    L  LS  G ++  F    
Sbjct: 70  MLAGLILGPQLFNLREVSSRKLSWDP---------ALDGNGPLRGLSVCGNIMLAFFMTV 129

Query: 142 KMDVRMILKTGRKAWIIGIPSTLIPIICGFWVGSYFLDGLSP--AEKNRV----PVIVSI 201
           K+  R+    G    +IG  S ++P + GF V +   D + P     N+V     V++S 
Sbjct: 130 KISRRLAFNNGWLPIVIGTLSFIVPFLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISS 189

Query: 202 QSFISFPVIASLLSELKMVSTELGRLGLSSALIADLLGQFVMATANQIRISRS-NITLGY 261
           QS I  P +   LSELK++++ELGRL LS++LI D+    V   A  +   ++ +    Y
Sbjct: 190 QSSILLPTVVHFLSELKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNISPMTAY 249

Query: 262 FNKAGIMVQFCLIIFVLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPA 321
            +   +++   +   VLRP V WI+++TPEGKPV+    H V + V+ SA  S+      
Sbjct: 250 RDLIAVIILILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMKY 309

Query: 322 VFGPYLLGLAIPDGASGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAF 381
           + GP+LLG+ IP+G   +G +L  K E    +  +P+ +   +++ DV K+   +    +
Sbjct: 310 LLGPFLLGIIIPEGPP-IGSALEAKYEALTMNVLIPISITFSTMRCDVMKIVYQYDDIWY 369

Query: 382 ARINIIITLVTYGVKLVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKII 441
              NI +   T  +K+    +  LYCK+P ++    SL+L  K   E+  Y  T+    I
Sbjct: 370 ---NIFLMTFTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDSYI 429

Query: 442 NHGILAWFILFLLTIATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNE 501
           +     + I   L  +  +P  +  L DPKR +  +Q +NIM+L P+S+LRIL CIH+ E
Sbjct: 430 SQATYTFLITCALINSGIIPTALAGLYDPKRKYVGYQKKNIMNLKPDSDLRILTCIHRPE 489

Query: 502 HIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLS 561
           +I   I  L     T    + V  LHL++LVG+T P+ ISH ++   +    Y     L+
Sbjct: 490 NISAAISFLQFLPST----IVVTVLHLVKLVGKTVPVLISHNKQINRVVTNSYIHTANLA 549

Query: 562 FDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQE 621
           F   E      SV    FT IT    M ++ICK+AL++ TS+II+P  R WT DG  + E
Sbjct: 550 FSQLE------SVTMTMFTAITHENLMHDEICKVALEQATSIIIVPSGRKWTVDGAFESE 609

Query: 622 DNMLRNLNCSVLQKADCSVAIFADKGHLGSIASMASSGT-QFRYSVCLIYLGGSDDREAI 681
           D  +R LN S+L+ A CS+ I  D+G L      +  GT +F   V +I++GG DDREA+
Sbjct: 610 DEAIRRLNESLLKSASCSIGILVDRGQL------SLKGTRKFNIDVGVIFIGGKDDREAL 669

Query: 682 SFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVC 741
           S  K+M + P +++TV++L S  E   S+ W+ +LD EVL+D K +      + Y E + 
Sbjct: 670 SLVKKMKQNPRVKITVIRLISDRET-ESTNWDYILDHEVLEDLK-DTEATNSIAYTERIV 729

Query: 742 DDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTR 786
             GP+ A T+R +  ++DLM+VGR  G+ SP   GL EW E PELGV+GDL+AS + ++R
Sbjct: 730 TGGPEVATTVRSLSEDYDLMVVGRDHGMASPDFDGLMEWVELPELGVIGDLLASRELDSR 763

BLAST of MS000462 vs. TAIR 10
Match: AT3G44930.1 (cation/H+ exchanger 10 )

HSP 1 Score: 420.6 bits (1080), Expect = 5.2e-117
Identity = 263/690 (38.12%), Postives = 393/690 (56.96%), Query Frame = 0

Query: 812  LGLLAEFGYTLYLFLSGVKIDLSMTIRAGKNALIIGILALLVPLFAANFVRSLLVDG--- 871
            L  ++ FG  ++ FL  V+    +   +GK  ++IGI++   PLF   F ++   D    
Sbjct: 103  LRCISVFGTLMFTFLMTVRTSRRVAFHSGKLPVVIGIVSFFAPLFGLGF-QNFFSDNIDP 162

Query: 872  --SGISEADLSTLPILISFHSMSSFPVIASLLSDLEIVNSELGRLGLSSALFSDLLSLFM 931
                +++A L     ++   S    P    +L +L+I+NSELGRL LS+ + +D+L +F 
Sbjct: 163  HYMPLTKA-LGERTAIVITQSSILLPSTTYILLELKIINSELGRLALSACVINDILGIFS 222

Query: 932  MVTAR-QAKRFKDVPSIASLQLGALILLFLLILFVFRPAMLWIIRQTPEGMPVKGSYIQA 991
            M+ A  QA       + A     A+I+ FL++  VF+P + W+I +TPE  PV+  YI A
Sbjct: 223  MIVASIQATYIHVSHATAYRDTVAVIIFFLVVFLVFKPMVQWVIDRTPEDKPVEDMYIHA 282

Query: 992  VIFLVLFTTVLFNFTGQISIMGPYVFGLAVPDGAPLASTLVNNIECLVSDVFMPILVITC 1051
            VI   L +   F F     I+GP + G+ +P+G PL S L    E L  +VF+PI +   
Sbjct: 283  VIITALASAAYFVFFNMKYILGPLMIGIIIPEGPPLGSALEAKFERLTMNVFLPISITFS 342

Query: 1052 ALKADLSQISSSFDAAFTKLNIVLILVTFVVKIVSCFLTSRHCRLPFRDSLALSLIMSNK 1111
            A++ D ++I S F+  F   NI L  +  V+K+V+C     + +LP  +SLA+S I+S K
Sbjct: 343  AMRCDGARILSQFNDIF--FNIFLTFLILVIKLVACLAPCLYYKLPLSESLAVSFILSYK 402

Query: 1112 GAVELVLYTMARDYNAINDGLLGWCFLSIVFVATLVPILVKCLYDPSMKYAGYQNRNIMH 1171
               + VLY    D   I+     +  L  +  A +VP +++ +YDP  KY  YQ R+I+H
Sbjct: 403  SFADFVLYEAVLDDTYISQATYSFLILYSLLNAGIVPTVLRRMYDPRRKYVNYQKRDILH 462

Query: 1172 LNPASDKLRLLACIHQNENINSIIHLLN-LSCPTAENPLAIHILHLIELSGRTAPVFISH 1231
            L   SD LR+L C+H+ EN++  I  L  LS P  + P+A+ +LHL++L G+  P+ +SH
Sbjct: 463  LERNSD-LRILTCLHKPENVSETIAFLQLLSSPNLDFPIAVTVLHLVKLVGQINPIIVSH 522

Query: 1232 KYFHNKALNDRSYSQHIIHSFDQFERDSDGTVCVECFTAISPNRLMHNDICTLALDKITS 1291
                 K LN  SY      +F QF  +S  +V V  FTA S   LMH DICTLALDK TS
Sbjct: 523  DK-KLKRLNKDSYIHTANLAFRQFVLESLESVTVTTFTAFSHENLMHEDICTLALDKTTS 582

Query: 1292 LIIMPFHRTWTVDGFAYHDDNMVRTLNCSVLGRAPCSIGIFADRGRLGSIAATAIPRSRY 1351
            +I++P  R WTVDG    D+  +R LN S+L RAPCSIGI  DRG+  S  +    + RY
Sbjct: 583  MIVVPSGRKWTVDGLFESDNTAIRHLNQSLLDRAPCSIGILVDRGQF-SRKSIVTSKKRY 642

Query: 1352 --SVCVLFLGGKDDREAISYAKRMAKDSRVELTMLRLRGPPEEHGQKTDNWEDILDAEVL 1411
               V VLF+GGKDDREA+S  KRM  + R+ +T++RL     +H  ++D W+ ILD E L
Sbjct: 643  IIDVGVLFIGGKDDREALSLVKRMKNNPRIRVTVIRL---VFDHEIESD-WDYILDNEGL 702

Query: 1412 RDFRSKCLGDGRVVYKEDVCRDGQQTAFIIRKIVNLFDLMIVGRRNGLKSSQTDGLNEWN 1471
            +D +S    +  + Y E +     +    ++ +   +DLM+VGR + + S    GL EW 
Sbjct: 703  KDLKS-TEDNKDIDYIERIVTSSVEVVKAVQLLAEEYDLMVVGRDHDMTSQDLSGLMEWV 762

Query: 1472 EFPELGVLGDLIASTDINSRASLLVIQQQQ 1493
            E PELGV+GDL+A+ D++S+ S+LV+QQQQ
Sbjct: 763  ELPELGVIGDLLAARDLSSKVSVLVVQQQQ 780


HSP 2 Score: 417.9 bits (1073), Expect = 3.4e-116
Identity = 259/770 (33.64%), Postives = 412/770 (53.51%), Query Frame = 0

Query: 29  VGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKISSQIIAGLVV 88
           + S G W N  + +     SLPLLE+Q+   F  + + H+  +  G+ +I+S +IAG+V+
Sbjct: 17  ISSQGFWDNLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGISQIASYMIAGIVL 76

Query: 89  GCS-WGPLDDTKFILF---RINSEEILGLLSYFGYMLFLFITAAKMDVRMILKTGRKAWI 148
           G   +  L+ +   L     ++    L  +S FG ++F F+   +   R+   +G+   +
Sbjct: 77  GPQLFDVLEKSSGKLSVDPALDGIAALRCISVFGTLMFTFLMTVRTSRRVAFHSGKLPVV 136

Query: 149 IGIPSTLIPIICGFWVGSYFLDGLSP-------AEKNRVPVIVSIQSFISFPVIASLLSE 208
           IGI S   P+  G    ++F D + P       A   R  ++++ QS I  P    +L E
Sbjct: 137 IGIVSFFAPLF-GLGFQNFFSDNIDPHYMPLTKALGERTAIVIT-QSSILLPSTTYILLE 196

Query: 209 LKMVSTELGRLGLSSALIADLLGQFVMATAN-QIRISRSNITLGYFNKAGIMVQFCLIIF 268
           LK++++ELGRL LS+ +I D+LG F M  A+ Q      +    Y +   +++ F ++  
Sbjct: 197 LKIINSELGRLALSACVINDILGIFSMIVASIQATYIHVSHATAYRDTVAVIIFFLVVFL 256

Query: 269 VLRPSVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGA 328
           V +P V W++ +TPE KPV     H V I  L SA          + GP ++G+ IP+G 
Sbjct: 257 VFKPMVQWVIDRTPEDKPVEDMYIHAVIITALASAAYFVFFNMKYILGPLMIGIIIPEGP 316

Query: 329 SGLGFSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVK 388
             LG +L  K E    + F+P+ +   +++ D +++   F    F   NI +T +   +K
Sbjct: 317 P-LGSALEAKFERLTMNVFLPISITFSAMRCDGARILSQFNDIFF---NIFLTFLILVIK 376

Query: 389 LVCCFLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTI 448
           LV C    LY KLPL +   +S ILS K   +   Y        I+    ++ IL+ L  
Sbjct: 377 LVACLAPCLYYKLPLSESLAVSFILSYKSFADFVLYEAVLDDTYISQATYSFLILYSLLN 436

Query: 449 ATFVPLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNEHIYGLIHLLN-LSCP 508
           A  VP  ++ + DP+R + ++Q R+I+HL  NS+LRIL C+HK E++   I  L  LS P
Sbjct: 437 AGIVPTVLRRMYDPRRKYVNYQKRDILHLERNSDLRILTCLHKPENVSETIAFLQLLSSP 496

Query: 509 TPQNPLAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVY 568
               P+AV  LHL++LVG+  PI +SH ++ K +    Y     L+F  F  + S  SV 
Sbjct: 497 NLDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLNKDSYIHTANLAFRQFVLE-SLESVT 556

Query: 569 AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 628
              FT  +    M  DIC LALDK TS+I++P  R WT DGL + ++  +R+LN S+L +
Sbjct: 557 VTTFTAFSHENLMHEDICTLALDKTTSMIVVPSGRKWTVDGLFESDNTAIRHLNQSLLDR 616

Query: 629 ADCSVAIFADKGHLGSIASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 688
           A CS+ I  D+G   S  S+ +S  ++   V ++++GG DDREA+S  KRM   P I +T
Sbjct: 617 APCSIGILVDRGQF-SRKSIVTSKKRYIIDVGVLFIGGKDDREALSLVKRMKNNPRIRVT 676

Query: 689 VLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVN 748
           V++L    +    S W+ +LD+E LKD K +   N  + Y E +     +    ++ +  
Sbjct: 677 VIRL--VFDHEIESDWDYILDNEGLKDLK-STEDNKDIDYIERIVTSSVEVVKAVQLLAE 736

Query: 749 EFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLI 786
           E+DLM+VGR   + S   +GL EW E PELGV+GDL+A+ D +++ SVL+
Sbjct: 737 EYDLMVVGRDHDMTSQDLSGLMEWVELPELGVIGDLLAARDLSSKVSVLV 775

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014725.10.0e+0053.69Cation/H(+) antiporter 3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022141218.10.0e+0099.62cation/H(+) antiporter 3-like [Momordica charantia][more]
TYK31711.10.0e+0052.23cation/H(+) antiporter 10-like [Cucumis melo var. makuwa][more]
KAA0025160.10.0e+0051.31cation/H(+) antiporter 10-like [Cucumis melo var. makuwa][more]
XP_008439404.20.0e+0050.44PREDICTED: uncharacterized protein LOC103484221 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q9FYC15.6e-14040.96Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1[more]
Q9FFB88.6e-13339.16Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9FYB99.9e-12138.72Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2[more]
Q9FYC03.5e-11834.01Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2[more]
Q58P697.3e-11638.12Cation/H(+) antiporter 10 OS=Arabidopsis thaliana OX=3702 GN=CHX10 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CJU70.0e+0099.62cation/H(+) antiporter 3-like OS=Momordica charantia OX=3673 GN=LOC111011670 PE=... [more]
A0A5A7SGA00.0e+0051.31Cation/H(+) antiporter 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A5D3E7Y00.0e+0052.23Cation/H(+) antiporter 10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3AYQ20.0e+0050.44uncharacterized protein LOC103484221 OS=Cucumis melo OX=3656 GN=LOC103484221 PE=... [more]
A0A6J1CI530.0e+0088.87cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111011605 PE=... [more]
Match NameE-valueIdentityDescription
AT3G44900.13.9e-14140.96cation/H+ exchanger 4 [more]
AT5G22900.16.1e-13439.16cation/H+ exchanger 3 [more]
AT3G44920.17.0e-12238.72cation/H+ exchanger 11 [more]
AT3G44910.12.5e-11934.01cation/H+ exchanger 12 [more]
AT3G44930.15.2e-11738.12cation/H+ exchanger 10 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 781..1155
e-value: 3.2E-47
score: 163.2
coord: 52..457
e-value: 5.5E-52
score: 178.9
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 783..1144
e-value: 1.1E-27
score: 96.9
coord: 59..447
e-value: 4.2E-29
score: 101.5
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 20..785
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 786..1493
NoneNo IPR availablePANTHERPTHR32468:SF17CATION/H(+) ANTIPORTER 4coord: 20..785
coord: 786..1493

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000462.1MS000462.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity