MS000458 (gene) Bitter gourd (TR) v1

Overview


Name	MS000458
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin-conjugating enzyme E2 protein
Location	scaffold44: 1832793 .. 1835157 (+)
RNA-Seq Expression	MS000458
Synteny	MS000458

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCGAAGAGGATTTTGAAGGAACTCAAGGATTTGCAGAAGGACCCTCCTACATCATGTAGCGCTGGTATGGAATTAATTTCTTAAAGAAAAAAAAAATTGGTGGCTGGGGGATGTTTTCTTCTTTGGATTGATTTATTTCCCTTGGCCTAATTCGTCCTTTAGATTGATTGGTCTTACAATATTCATCGTTCTCTTTTGCTACTTGCCTTCGTGTGGATATGTATTCTTTCTAGAGAGTCAATTTTTCTGCTGTACTTGTAGATTATCCACCCTTCGTGGTTTCTGTTGAGAATATATGCTAGTAATTATCTCTAGATAGGGAAAGGTTGTTGGCTGAAATTTGTTAGAGGTTTCTTTACCAACTGATCGTAGAATAATCATGTTAATGTGGTTTATGAAAGGGGAGAATTGAGTCAATGAATCCCGTGTAAAATATTCCGAGAATTTGGTTCTATTGTGTGTCTGAGCATCTCAATTGTTCAACTTCGAATCTTTAGACGATTTCATGTGGAAGTTCCACTCATACAGAAGAAATTTTATGCACTTCTTCATGTGTTCACATCTGGAACTAAAATTAAGATATTACAGATTGCGACTATTTTCATTTTTCCTTTATTTTGTAATTTGACTTCAGTAATTACGTTTTAAGTAGTTTAGAACTTCCAGCTCCTGGTTAGCTTCAGTACTTCTTGCTCTAAAGACAAAGTTTCAGGTTATAACATGAACATTGGTTGATTGGATATGAATGGCGAGATGGTAACTATATTGACTTGTCCTAAGATTTGTTAATACATTGAGATTTAGTTTTGATCCTGTATGGTTTTCAATCAGTTGGTTTCTATTGAGGTGCTGACTCATGTAAGAATTTTTGCAGGTCCAGTTGCCGAAGACATGTTTCATTGGCAAGCTACCATCATGGGTCCTCCAGACAGTCCTTATGCTGGGGGTGTTTTCCTTGTCACCATTCATTTCCCCCCAGATTATCCTTTTAAGCCTCCCAAGGTGTGACAGCTTCTTTTAAGTTGCTGTTTACTCTAAGCCTATGTGTCCTATATTTTATAAAATATGCTGAATATCTCGATCTTGCCTTTTATTTTCTTATCTGTTCTTTAAGATAAACTGTTTTCAGCCTAAGGGTCTGAATTGGGTAATTTGTTTGTAACTAAGTTTAGCTGTATTGAGCTCAACTAACAGAGTGAATTCCCTTTGATATTTGGCCATGCATTTTCTCCTTCATTTGATAGTGTTATTCAATTATGCATGCAATTATTTTTATAATTGTTAGATGTAAGTTCTCCAGATAGGTTAATGTGCTGCCTTCAGATTGTCTTTATTAAATAAAAGGGAGTGGTCCAAGGCATAGATGCTGTTTAGGCTAGATTCTTTGTCACGCCCTGTCATCTGTTGGTTTCTTTATTTTTCTGGTTAGGGTTTAACATGTAATTTGATGAATTGTGACTACGTGTGCAAATGTTTCTTTCTTGCCTAGGGAACATTTCCTCCTTTCTTGTAATTGACTTCTTTAACATAGAAATGTTTCTTGTCATGGATTTTCTTGGAAGCGGCTTTCTTATTATTTCCTCCTGTCTCATAATTTAATGTATTTTCAGGTGGCATTTAGAACAAAGGTGTTCCACCCAAATATCAATAGCAATGGTAGTATTTGCCTTGACATTTTGAAAGAGCAGTGGAGCCCTGCCCTAACTATTTCCAAGGTTTGTCCCTTGACTTGATTAAGAAAAAAAGAGGTTCTGTTTAAGACTATGATATGGTCCTCTTTGCAAATGCATACTAGTTTTCGTACCTATCTCCCCAAAACCTTTTTTTCCGCCTGTTCTCTTAACATTGTAACGTCTGTACTAGCATCCAGATGCTATTTGAAAGATGGTGCAAACATTGACCGGCATCTCTTTTTCGGACTTAGATAGGAGAAATAACTCAACTAAGTAGAACAAACCTAACCTTTTTGTCCAATTGTTATTGAAGAATATCTTCGTGGGCTAGTCTCCATCAACAATATGGCCCTTGATATGGGATGGGATCGGCTCCCTTGACTCCATATATTCATATTGAATTGGATTCATCTTGATTGATTCTATAACCCCTTTTTTGGCAATATGTCACGGACATCTCCTGGTTGTACCTTGATATTTTTCTATAATGCGTCCTTGTGTTCATGATTACTGAAACAGCAGTTTCCTTGTATGTTTAAAACTAGGTCTTACTTTCGATCTGTTCGCTGTTGACGGACCCAAATCCTGATGACCCGCTGGTGCCCGAGATTGCTCACATGTACAAGACTGACAGGAACAAATACGAGACGACTGCCAGGAGCTGGACGCAGAAGTATGCGATGGGC

mRNA sequence

ATGGCGTCGAAGAGGATTTTGAAGGAACTCAAGGATTTGCAGAAGGACCCTCCTACATCATGTAGCGCTGGTCCAGTTGCCGAAGACATGTTTCATTGGCAAGCTACCATCATGGGTCCTCCAGACAGTCCTTATGCTGGGGGTGTTTTCCTTGTCACCATTCATTTCCCCCCAGATTATCCTTTTAAGCCTCCCAAGGTGGCATTTAGAACAAAGGTGTTCCACCCAAATATCAATAGCAATGGTAGTATTTGCCTTGACATTTTGAAAGAGCAGTGGAGCCCTGCCCTAACTATTTCCAAGGTCTTACTTTCGATCTGTTCGCTGTTGACGGACCCAAATCCTGATGACCCGCTGGTGCCCGAGATTGCTCACATGTACAAGACTGACAGGAACAAATACGAGACGACTGCCAGGAGCTGGACGCAGAAGTATGCGATGGGC

Coding sequence (CDS)

ATGGCGTCGAAGAGGATTTTGAAGGAACTCAAGGATTTGCAGAAGGACCCTCCTACATCATGTAGCGCTGGTCCAGTTGCCGAAGACATGTTTCATTGGCAAGCTACCATCATGGGTCCTCCAGACAGTCCTTATGCTGGGGGTGTTTTCCTTGTCACCATTCATTTCCCCCCAGATTATCCTTTTAAGCCTCCCAAGGTGGCATTTAGAACAAAGGTGTTCCACCCAAATATCAATAGCAATGGTAGTATTTGCCTTGACATTTTGAAAGAGCAGTGGAGCCCTGCCCTAACTATTTCCAAGGTCTTACTTTCGATCTGTTCGCTGTTGACGGACCCAAATCCTGATGACCCGCTGGTGCCCGAGATTGCTCACATGTACAAGACTGACAGGAACAAATACGAGACGACTGCCAGGAGCTGGACGCAGAAGTATGCGATGGGC

Protein sequence

MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYAMG

Homology

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BLAST of MS000458 vs. NCBI nr
Match: TKS17383.1 (hypothetical protein D5086_0000014670 [Populus alba])

HSP 1 Score: 314.3 bits (804), Expect = 5.6e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 18  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 77

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 78  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 137

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 138 PEIAHMYKTDRNKYETTARSWTQKYAMG 165

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BLAST of MS000458 vs. NCBI nr
Match: KAF5451120.1 (hypothetical protein F2P56_031415, partial [Juglans regia])

HSP 1 Score: 314.3 bits (804), Expect = 5.6e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 52  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 111

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 112 PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 171

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 172 PEIAHMYKTDRNKYETTARSWTQKYAMG 199

[Close]

BLAST of MS000458 vs. NCBI nr
Match: KAB5524173.1 (hypothetical protein DKX38_021922 [Salix brachista] >KAB5524196.1 hypothetical protein DKX38_021945 [Salix brachista])

HSP 1 Score: 314.3 bits (804), Expect = 5.6e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 15  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 74

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 75  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 134

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 135 PEIAHMYKTDRNKYETTARSWTQKYAMG 162

[Close]

BLAST of MS000458 vs. NCBI nr
Match: KAF5451122.1 (hypothetical protein F2P56_031417 [Juglans regia])

HSP 1 Score: 314.3 bits (804), Expect = 5.6e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 28  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 87

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 88  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 147

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 148 PEIAHMYKTDRNKYETTARSWTQKYAMG 175

[Close]

BLAST of MS000458 vs. NCBI nr
Match: KAF3432883.1 (hypothetical protein FNV43_RR23985 [Rhamnella rubrinervis])

HSP 1 Score: 314.3 bits (804), Expect = 5.6e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 220 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 279

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 280 PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 339

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 340 PEIAHMYKTDRNKYETTARSWTQKYAMG 367

[Close]

BLAST of MS000458 vs. ExPASy Swiss-Prot
Match: P35133 (Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana OX=3702 GN=UBC10 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.2e-82
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP +SPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTD+NKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

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BLAST of MS000458 vs. ExPASy Swiss-Prot
Match: P35135 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 1.5e-82
Identity = 145/148 (97.97%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP DSPYAGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDR KYETTARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYETTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. ExPASy Swiss-Prot
Match: P35132 (SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 2.0e-82
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP DSPY+GGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTD+NKYE+TAR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARTWTQKYAMG 148

[Close]

BLAST of MS000458 vs. ExPASy Swiss-Prot
Match: Q94F47 (Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 4.4e-82
Identity = 143/148 (96.62%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP DSPY+GGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDR KYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. ExPASy Swiss-Prot
Match: Q8S919 (Ubiquitin-conjugating enzyme E2 5B OS=Oryza sativa subsp. japonica OX=39947 GN=UBC5B PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 2.9e-81
Identity = 142/148 (95.95%), Postives = 145/148 (97.97%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQAT+MGP DSPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATLMGPSDSPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVA +TKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVALKTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDR KYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. ExPASy TrEMBL
Match: A0A059CHQ6 (UBC core domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_D01776 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.7e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. ExPASy TrEMBL
Match: A0A6J1J9S3 (ubiquitin-conjugating enzyme E2 10 OS=Cucurbita maxima OX=3661 GN=LOC111483017 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.7e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. ExPASy TrEMBL
Match: A0A5D2FJ63 (UBC core domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES288_A08G121400v1 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.7e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. ExPASy TrEMBL
Match: A0A5N5K2Z8 (UBC core domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38_021922 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.7e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 15  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 74

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 75  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 134

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 135 PEIAHMYKTDRNKYETTARSWTQKYAMG 162

[Close]

BLAST of MS000458 vs. ExPASy TrEMBL
Match: M5XDZ1 (UBC core domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G306300 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.7e-82
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY
Sbjct: 13  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 72

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 73  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 132

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTDRNKYETTARSWTQKYAMG
Sbjct: 133 PEIAHMYKTDRNKYETTARSWTQKYAMG 160

[Close]

BLAST of MS000458 vs. TAIR 10
Match: AT5G53300.1 (ubiquitin-conjugating enzyme 10 )

HSP 1 Score: 307.0 bits (785), Expect = 8.3e-84
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP +SPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTD+NKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. TAIR 10
Match: AT5G53300.2 (ubiquitin-conjugating enzyme 10 )

HSP 1 Score: 307.0 bits (785), Expect = 8.3e-84
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP +SPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTD+NKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. TAIR 10
Match: AT5G53300.4 (ubiquitin-conjugating enzyme 10 )

HSP 1 Score: 307.0 bits (785), Expect = 8.3e-84
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP +SPYAGGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSESPYAGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTD+NKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARSWTQKYAMG 148

[Close]

BLAST of MS000458 vs. TAIR 10
Match: AT4G27960.1 (ubiquitin conjugating enzyme 9 )

HSP 1 Score: 306.2 bits (783), Expect = 1.4e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP DSPY+GGVFLVTIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTD+NKYE+TAR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARTWTQKYAMG 148

[Close]

BLAST of MS000458 vs. TAIR 10
Match: AT4G27960.2 (ubiquitin conjugating enzyme 9 )

HSP 1 Score: 306.2 bits (783), Expect = 1.4e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDY 60
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP DSPY+GGVFLVTIHFPPDY
Sbjct: 31  MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 90

Query: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 91  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 150

Query: 121 PEIAHMYKTDRNKYETTARSWTQKYAMG 149
           PEIAHMYKTD+NKYE+TAR+WTQKYAMG
Sbjct: 151 PEIAHMYKTDKNKYESTARTWTQKYAMG 178

The following BLAST results are available for this feature:

BLAST of MS000458 vs. NCBI nr


Match Name	E-value	Identity	Description
TKS17383.1	5.6e-82	100.00	hypothetical protein D5086_0000014670 [Populus alba]	[more]
KAF5451120.1	5.6e-82	100.00	hypothetical protein F2P56_031415, partial [Juglans regia]	[more]
KAB5524173.1	5.6e-82	100.00	hypothetical protein DKX38_021922 [Salix brachista] >KAB5524196.1 hypothetical p...	[more]
KAF5451122.1	5.6e-82	100.00	hypothetical protein F2P56_031417 [Juglans regia]	[more]
KAF3432883.1	5.6e-82	100.00	hypothetical protein FNV43_RR23985 [Rhamnella rubrinervis]	[more]

BLAST of MS000458 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P35133	1.2e-82	97.30	Ubiquitin-conjugating enzyme E2 10 OS=Arabidopsis thaliana OX=3702 GN=UBC10 PE=1...	[more]
P35135	1.5e-82	97.97	Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1	[more]
P35132	2.0e-82	96.62	SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1	[more]
Q94F47	4.4e-82	96.62	Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1...	[more]
Q8S919	2.9e-81	95.95	Ubiquitin-conjugating enzyme E2 5B OS=Oryza sativa subsp. japonica OX=39947 GN=U...	[more]

BLAST of MS000458 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A059CHQ6	2.7e-82	100.00	UBC core domain-containing protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_D01...	[more]
A0A6J1J9S3	2.7e-82	100.00	ubiquitin-conjugating enzyme E2 10 OS=Cucurbita maxima OX=3661 GN=LOC111483017 P...	[more]
A0A5D2FJ63	2.7e-82	100.00	UBC core domain-containing protein OS=Gossypium darwinii OX=34276 GN=ES288_A08G1...	[more]
A0A5N5K2Z8	2.7e-82	100.00	UBC core domain-containing protein OS=Salix brachista OX=2182728 GN=DKX38_021922...	[more]
M5XDZ1	2.7e-82	100.00	UBC core domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_2G306300 P...	[more]

BLAST of MS000458 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G53300.1	8.3e-84	97.30	ubiquitin-conjugating enzyme 10	[more]
AT5G53300.2	8.3e-84	97.30	ubiquitin-conjugating enzyme 10	[more]
AT5G53300.4	8.3e-84	97.30	ubiquitin-conjugating enzyme 10	[more]
AT4G27960.1	1.4e-83	96.62	ubiquitin conjugating enzyme 9	[more]
AT4G27960.2	1.4e-83	96.62	ubiquitin conjugating enzyme 9	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 4..147 e-value: 9.6E-81 score: 284.2
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 3..145
None	No IPR available	PANTHER	PTHR24068:SF346	BNAA01G32320D PROTEIN	coord: 3..145
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 1..148 e-value: 6.9E-76 score: 255.3
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 2..147
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 5..141 e-value: 4.0E-54 score: 182.1
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 1..147 score: 61.741959
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 3..142 e-value: 1.12189E-75 score: 219.766
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 74..89

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000458.1	MS000458.1	mRNA