MS000450 (gene) Bitter gourd (TR) v1

Overview


Name	MS000450
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	NADH dehydrogenase
Location	scaffold44: 1733843 .. 1735188 (+)
RNA-Seq Expression	MS000450
Synteny	MS000450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAAGGTGAAGCAGACGACCGGGATCGTAGGTCTGGAGGTCGTCCCTAACGCGAGAGAGGTATTGATCGGTCTTTACTCTAAAACCCTAAAGGAGATCCAGGCTGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACGCGCCACCGCTTGAAGGTTTGCCAGGAGGAAGACGACTGGGAGAAAATTGAGAAGCGGCTTGGCTGTGGCCAGGTGGAGGAGCTCATCGAGGAGGCTCAGGACGAGCTCAAGCTCATTGCTAAAATGATCGGTATGTATTTAATTTTTATATGCAGTCAATTCCCGTTTCATTTTCATTTGAATTCTGGTTCCGTTTACGATGGAATGTAAGAATTGGTAAAGGAATCTAGCAGGATTTGGGAGTTATATAATGTTTATATCTCTGCATTGCGTCGAAATGATGAAAACCGAACCTTTTTAGCTCAATTTCCGTAGGAAAACTCACGACTGCGAACTTTGAGGTGTTTGTTATTGTGAGTTTTGTATGTTGATGAGAACTTGTAGGGAATAGGATGGAGATTCGCGATTTAATTTAACAAGTATTGAGTGTCAAAGAATGCCATTTGCATCTTGTGTTGTGCTGGAAAAAGTGGGTTACTAATACTTAAAGATTAGACTGTAATTATCCTTCTTCCATCGTTACCCTCTTCTTGGCTCGTTGAATCATTGGCCTCATGGCGTAATGGCAATCCCCGATTTCATTTTTCTCTTAACCTGGGGTCATTGGCTGTAAGATTAGTTGTCTACTCATTTTTTTCTGTAAGATTAGTATCGTACTAGATTTATATTTTCTCAATAGATGAATCTGAGGTCATTCGCTGGACTGTCTCCTTGAGCATCATTTGGGTAATTGGATTACTACCTAACGTCCTGAAGTGTTAATGTCTCTTAACTTCTGAAGTTGATTTCTTGTTCTCACCAAGATGAATGAATGAATATCGCATATTCAAAGGAATCATGATCTAGTCTGGAAGTTTGAAATCTCTTCAGTGATGACAGAAATTTTATAGATAGAGATTTACCGACTTCTTTTTTCCAGTTATCACAATATTTCTATAGTTATGTTATCAATTAGTTATCTCAAATATGCAGAGTGGGATCCCTGGGGTGTTCCTGATGACTATGAATGTGAAGTGATCGAGAACGACGCTCCGGTACCCAAGCATATTCCTCTGCACCGACCAGGTCCTCTCCCGGAAGAGTTCTACAAAACGCTGGAAGCCATCGGTGGTGGTAGTACGCAAAAGGTAGAGACAGCCGAAAAAGCATCTCAGGTGACAGAG

mRNA sequence

ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAAGGTGAAGCAGACGACCGGGATCGTAGGTCTGGAGGTCGTCCCTAACGCGAGAGAGGTATTGATCGGTCTTTACTCTAAAACCCTAAAGGAGATCCAGGCTGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACGCGCCACCGCTTGAAGGTTTGCCAGGAGGAAGACGACTGGGAGAAAATTGAGAAGCGGCTTGGCTGTGGCCAGGTGGAGGAGCTCATCGAGGAGGCTCAGGACGAGCTCAAGCTCATTGCTAAAATGATCGAGTGGGATCCCTGGGGTGTTCCTGATGACTATGAATGTGAAGTGATCGAGAACGACGCTCCGGTACCCAAGCATATTCCTCTGCACCGACCAGGTCCTCTCCCGGAAGAGTTCTACAAAACGCTGGAAGCCATCGGTGGTGGTAGTACGCAAAAGGTAGAGACAGCCGAAAAAGCATCTCAGGTGACAGAG

Coding sequence (CDS)

ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAAGGTGAAGCAGACGACCGGGATCGTAGGTCTGGAGGTCGTCCCTAACGCGAGAGAGGTATTGATCGGTCTTTACTCTAAAACCCTAAAGGAGATCCAGGCTGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACGCGCCACCGCTTGAAGGTTTGCCAGGAGGAAGACGACTGGGAGAAAATTGAGAAGCGGCTTGGCTGTGGCCAGGTGGAGGAGCTCATCGAGGAGGCTCAGGACGAGCTCAAGCTCATTGCTAAAATGATCGAGTGGGATCCCTGGGGTGTTCCTGATGACTATGAATGTGAAGTGATCGAGAACGACGCTCCGGTACCCAAGCATATTCCTCTGCACCGACCAGGTCCTCTCCCGGAAGAGTTCTACAAAACGCTGGAAGCCATCGGTGGTGGTAGTACGCAAAAGGTAGAGACAGCCGAAAAAGCATCTCAGGTGACAGAG

Protein sequence

MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE

Homology

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BLAST of MS000450 vs. NCBI nr
Match: XP_022141236.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Momordica charantia])

HSP 1 Score: 335.5 bits (859), Expect = 2.6e-88
Identity = 166/168 (98.81%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE 169
           ENDA VPKHIPLHRPGPLPEEFYKTLEAIGGGS+QKVETAEKASQVTE
Sbjct: 121 ENDASVPKHIPLHRPGPLPEEFYKTLEAIGGGSSQKVETAEKASQVTE 168

[Close]

BLAST of MS000450 vs. NCBI nr
Match: XP_008439300.1 (PREDICTED: probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucumis melo])

HSP 1 Score: 325.5 bits (833), Expect = 2.7e-85
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGL+VVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE+DWE IEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI G ST+KVET EKASQVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISGDSTRKVETTEKASQVTE 168

[Close]

BLAST of MS000450 vs. NCBI nr
Match: XP_038874963.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Benincasa hispida])

HSP 1 Score: 325.1 bits (832), Expect = 3.6e-85
Identity = 159/168 (94.64%), Postives = 164/168 (97.62%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLAKVKQTTGIVGL+VVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE+DWEKIE+RLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEKIEQRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAIG  S +KVETAEKA QVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAIGSDSAKKVETAEKAPQVTE 168

[Close]

BLAST of MS000450 vs. NCBI nr
Match: XP_004140748.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucumis sativus] >KGN57450.2 hypothetical protein Csa_010484 [Cucumis sativus])

HSP 1 Score: 323.9 bits (829), Expect = 8.0e-85
Identity = 159/168 (94.64%), Postives = 162/168 (96.43%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGL+VVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE+DWE IEKRLGCGQVEELIEEAQDELKLI KMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE 169
           ENDAPVPKHIPLHRPGPLPEEFYKTLEAI G ST+KVET EKASQVTE
Sbjct: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAISGDSTKKVETPEKASQVTE 168

[Close]

BLAST of MS000450 vs. NCBI nr
Match: XP_022945797.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita moschata] >XP_023542504.1 probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 322.4 bits (825), Expect = 2.3e-84
Identity = 159/169 (94.08%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLAKVKQTTGIVGL+VVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE+DWE+IEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAI-GGGSTQKVETAEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI  GGS QKVETA+KASQVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISSGGSAQKVETADKASQVTE 169

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BLAST of MS000450 vs. ExPASy Swiss-Prot
Match: Q9FLX7 (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g52840 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 1.9e-73
Identity = 133/160 (83.12%), Postives = 143/160 (89.38%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGL+VVPNAR VLI LYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TR RL VC+EE+DWE IEKRLGCGQVEELIEEA+DEL LI KMIEWDPWGVPDDYECEVI
Sbjct: 61  TRQRLNVCKEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETA 161
           ENDAP+PKH+P HRPGPLPE+FYKTLE +   S  ++  A
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEQFYKTLEGLIAESKTEIPAA 160

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BLAST of MS000450 vs. ExPASy Swiss-Prot
Match: Q559Z4 (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyostelium discoideum OX=44689 GN=ndufa5 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.8e-18
Identity = 46/107 (42.99%), Postives = 73/107 (68.22%), Query Frame = 0


Query: 14  VKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEDD 73
           +K++TGI GL+V PNAR +L GLY++ LK+++ +P   GYRK+ E  T+ RL V + E D
Sbjct: 6   IKKSTGITGLKVEPNARHILAGLYNQLLKKLEILPPTAGYRKSAELETKFRLGVIENETD 65

Query: 74  WEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 121
             KIE ++  GQ+EELI +A+++LK++  + E   W +PD  +  V+
Sbjct: 66  IVKIENKIYAGQIEELIVQAKNDLKVVDLVHESRAWELPDKNKPPVV 112

[Close]

BLAST of MS000450 vs. ExPASy Swiss-Prot
Match: P23935 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus OX=9913 GN=NDUFA5 PE=1 SV=3)

HSP 1 Score: 84.3 bits (207), Expect = 1.4e-15
Identity = 42/96 (43.75%), Postives = 62/96 (64.58%), Query Frame = 0


Query: 14  VKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEDD 73
           +K+TTG+VGL V     E L  LY+K L  +  +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCETPHERLKILYTKILDVLGHIPKNAAYRKYTEQITNEKLSMVKAEPD 64

Query: 74  WEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPW 110
            +K+E+RL  GQ+EE+I +A++EL L  KMI+W PW
Sbjct: 65  VKKLEERLQGGQIEEVILQAENELSLARKMIQWKPW 100

[Close]

BLAST of MS000450 vs. ExPASy Swiss-Prot
Match: Q9CPP6 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Mus musculus OX=10090 GN=Ndufa5 PE=1 SV=3)

HSP 1 Score: 79.3 bits (194), Expect = 4.5e-14
Identity = 41/96 (42.71%), Postives = 59/96 (61.46%), Query Frame = 0


Query: 14  VKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEDD 73
           +K+TTG+VGL V     E L  LY+KTL  ++  P+   YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCDTPHERLTILYTKTLDILKHFPKHAAYRKYTEQITNEKLDMVKAEPD 64

Query: 74  WEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPW 110
            +K+E  L  G+VEE+I +A+ EL L  KM++W PW
Sbjct: 65  VKKLEALLQGGEVEEVILQAEKELSLARKMLKWKPW 100

[Close]

BLAST of MS000450 vs. ExPASy Swiss-Prot
Match: Q4R5J1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Macaca fascicularis OX=9541 GN=NDUFA5 PE=3 SV=3)

HSP 1 Score: 78.6 bits (192), Expect = 7.7e-14
Identity = 40/96 (41.67%), Postives = 59/96 (61.46%), Query Frame = 0


Query: 14  VKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEDD 73
           +K+TTG+VGL V     E L  LY+K L  +  +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCSTPHERLSILYTKILDVLAEIPKNAAYRKYTEQITNEKLAMVKAEPD 64

Query: 74  WEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPW 110
            +K+E +L  GQ+EE+I +A+ EL L  KM +W PW
Sbjct: 65  VKKLEDQLQGGQLEEVILQAEHELSLARKMRDWKPW 100

[Close]

BLAST of MS000450 vs. ExPASy TrEMBL
Match: A0A6J1CII6 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011683 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.3e-88
Identity = 166/168 (98.81%), Postives = 167/168 (99.40%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE 169
           ENDA VPKHIPLHRPGPLPEEFYKTLEAIGGGS+QKVETAEKASQVTE
Sbjct: 121 ENDASVPKHIPLHRPGPLPEEFYKTLEAIGGGSSQKVETAEKASQVTE 168

[Close]

BLAST of MS000450 vs. ExPASy TrEMBL
Match: A0A1S3AZ47 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103484126 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.3e-85
Identity = 159/168 (94.64%), Postives = 163/168 (97.02%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGL+VVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE+DWE IEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI G ST+KVET EKASQVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISGDSTRKVETTEKASQVTE 168

[Close]

BLAST of MS000450 vs. ExPASy TrEMBL
Match: A0A6J1G1X4 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111449938 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.1e-84
Identity = 159/169 (94.08%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLAKVKQTTGIVGL+VVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE+DWE+IEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAI-GGGSTQKVETAEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI  GGS QKVETA+KASQVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISSGGSAQKVETADKASQVTE 169

[Close]

BLAST of MS000450 vs. ExPASy TrEMBL
Match: A0A6J1HXB9 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467715 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.5e-84
Identity = 159/170 (93.53%), Postives = 165/170 (97.06%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLAKVKQTTGIVGL+VVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TRHRLKVCQEE+DWE+IEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAI--GGGSTQKVETAEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI  GG S QKVETA+KASQVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISSGGSSAQKVETADKASQVTE 170

[Close]

BLAST of MS000450 vs. ExPASy TrEMBL
Match: A0A6J1JA00 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111482620 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.3e-84
Identity = 156/168 (92.86%), Postives = 161/168 (95.83%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGL+VVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           T HRLKVCQEE+DWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI
Sbjct: 61  TNHRLKVCQEEEDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETAEKASQVTE 169
           ENDAPVPKH+PLHRPGPLPEEFYKTLEAI G   QKV++AEK SQVTE
Sbjct: 121 ENDAPVPKHVPLHRPGPLPEEFYKTLEAISGDGAQKVDSAEKTSQVTE 168

[Close]

BLAST of MS000450 vs. TAIR 10
Match: AT5G52840.1 (NADH-ubiquinone oxidoreductase-related )

HSP 1 Score: 276.6 bits (706), Expect = 1.4e-74
Identity = 133/160 (83.12%), Postives = 143/160 (89.38%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLAKVKQTTGIVGL+VVPNAR VLI LYSKTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
           TR RL VC+EE+DWE IEKRLGCGQVEELIEEA+DEL LI KMIEWDPWGVPDDYECEVI
Sbjct: 61  TRQRLNVCKEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMIEWDPWGVPDDYECEVI 120

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGSTQKVETA 161
           ENDAP+PKH+P HRPGPLPE+FYKTLE +   S  ++  A
Sbjct: 121 ENDAPIPKHVPQHRPGPLPEQFYKTLEGLIAESKTEIPAA 160

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BLAST of MS000450 vs. TAIR 10
Match: AT4G28005.1 (BEST Arabidopsis thaliana protein match is: NADH-ubiquinone oxidoreductase-related (TAIR:AT5G52840.1); Has 37 Blast hits to 37 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 33; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 111.7 bits (278), Expect = 5.8e-25
Identity = 74/169 (43.79%), Postives = 87/169 (51.48%), Query Frame = 0


Query: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60
           MF RVIGRPLLAKVKQTTGIV                                       
Sbjct: 1   MFQRVIGRPLLAKVKQTTGIV--------------------------------------- 60

Query: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMIEWDPWGVPDDYECEVI 120
                +VC EE+DW  IEKRL CG+VEELIEEA DEL L+ KMI              +I
Sbjct: 61  -----EVCPEEEDWGVIEKRLACGKVEELIEEASDELTLVGKMI--------GSLHPNLI 115

Query: 121 ENDAPVPKHIPLHRPGPLPEEFYKTLEAIGGGS-TQKVETAEKASQVTE 169
              AP+PKH+P HRPGPLPEEFY+TL+A+   S T+   T+    Q+ E
Sbjct: 121 L--APIPKHVPHHRPGPLPEEFYRTLQAVNSESKTEITATSSSDPQLKE 115

The following BLAST results are available for this feature:

BLAST of MS000450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141236.1	2.6e-88	98.81	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
XP_008439300.1	2.7e-85	94.64	PREDICTED: probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5...	[more]
XP_038874963.1	3.6e-85	94.64	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
XP_004140748.1	8.0e-85	94.64	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
XP_022945797.1	2.3e-84	94.08	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]

BLAST of MS000450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX7	1.9e-73	83.13	Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
Q559Z4	1.8e-18	42.99	Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyos...	[more]
P23935	1.4e-15	43.75	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus OX=99...	[more]
Q9CPP6	4.5e-14	42.71	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Mus musculus OX=...	[more]
Q4R5J1	7.7e-14	41.67	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Macaca fascicula...	[more]

BLAST of MS000450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CII6	1.3e-88	98.81	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A1S3AZ47	1.3e-85	94.64	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A6J1G1X4	1.1e-84	94.08	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A6J1HXB9	1.5e-84	93.53	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A6J1JA00	3.3e-84	92.86	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]

BLAST of MS000450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52840.1	1.4e-74	83.13	NADH-ubiquinone oxidoreductase-related	[more]
AT4G28005.1	5.8e-25	43.79	BEST Arabidopsis thaliana protein match is: NADH-ubiquinone oxidoreductase-relat...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006806	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5	PFAM	PF04716	ETC_C1_NDUFA5	coord: 22..88 e-value: 4.3E-27 score: 93.7
IPR006806	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5	PANTHER	PTHR12653	NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT	coord: 1..164
None	No IPR available	PANTHER	PTHR12653:SF1	BNAA02G10640D PROTEIN	coord: 1..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000450.1	MS000450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022904	respiratory electron transport chain