MS000446 (gene) Bitter gourd (TR) v1

BLAST of MS000446 vs. NCBI nr
Match: XP_022141221.1 (uncharacterized protein LOC111011673 [Momordica charantia])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 339/340 (99.71%), Postives = 339/340 (99.71%), Query Frame = 0

Query: 1   ASSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMR 60
           ASSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMR
Sbjct: 2   ASSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMR 61

Query: 61  QSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTL 120
           QSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTL
Sbjct: 62  QSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTL 121

Query: 121 ASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVII 180
           ASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVII
Sbjct: 122 ASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVII 181

Query: 181 WNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKA 240
           WNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIV CATNSEIPLVKGEKLKA
Sbjct: 182 WNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVCCATNSEIPLVKGEKLKA 241

Query: 241 GAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLV 300
           GAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLV
Sbjct: 242 GAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLV 301

Query: 301 ELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           ELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI
Sbjct: 302 ELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341

BLAST of MS000446 vs. NCBI nr
Match: XP_038880486.1 (protein SAR DEFICIENT 4 [Benincasa hispida] >XP_038880489.1 protein SAR DEFICIENT 4 [Benincasa hispida])

HSP 1 Score: 546.2 bits (1406), Expect = 2.0e-151
Identity = 285/341 (83.58%), Postives = 314/341 (92.08%), Query Frame = 0

Query: 2   SSTPNNPKD--ETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPM 61
           +ST +NPKD  ETNL   +T+RSPMFI+KEALDSIL+HQ L EHF+SSLPIV  TI SPM
Sbjct: 2   ASTSSNPKDETETNLAKKITARSPMFITKEALDSILTHQRLSEHFQSSLPIVFHTIFSPM 61

Query: 62  RQSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQT 121
           RQSHAV P+SSLLLMPSWSSSPS+PYIGVKLV HFPQNSAINLPAIHASYSLFSSTTGQT
Sbjct: 62  RQSHAVQPHSSLLLMPSWSSSPSLPYIGVKLVIHFPQNSAINLPAIHASYSLFSSTTGQT 121

Query: 122 LASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVI 181
           LASMDGT LTLYRTSC+SGLAAKYLARPDS VMVMVGAGAL PHLIKAHLAARPSV KVI
Sbjct: 122 LASMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVNKVI 181

Query: 182 IWNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLK 241
           IWNRTEE+A+KLA+EM+KNAGL+GVCFESS NL+DAI  ADIVSCATNSE+PLVKG+KLK
Sbjct: 182 IWNRTEERAQKLADEMRKNAGLDGVCFESSANLDDAIPEADIVSCATNSEVPLVKGDKLK 241

Query: 242 AGAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNL 301
           AGA+L+LVGSF HSMRECDD+AIKRGRVFVD E A+EEAGELVGA ERGVIEKR+I+GNL
Sbjct: 242 AGAYLNLVGSFKHSMRECDDMAIKRGRVFVDGEEALEEAGELVGALERGVIEKRDILGNL 301

Query: 302 VELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           V+LIKGEVVGRR+G+EITVFKSVGSA+ DLV+AQ  YETTI
Sbjct: 302 VDLIKGEVVGRRNGDEITVFKSVGSAVFDLVAAQFAYETTI 342

BLAST of MS000446 vs. NCBI nr
Match: XP_023552312.1 (protein SAR DEFICIENT 4-like [Cucurbita pepo subsp. pepo] >XP_023552313.1 protein SAR DEFICIENT 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545.8 bits (1405), Expect = 2.6e-151
Identity = 280/339 (82.60%), Postives = 314/339 (92.63%), Query Frame = 0

Query: 2   SSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMRQ 61
           +S  NNPKD+T+LT  +T R PMFI+KE LDSIL HQNLIEHF+SSL IVSPTISSPMRQ
Sbjct: 2   ASPSNNPKDKTSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMRQ 61

Query: 62  SHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLA 121
           SH V P+SSLLLMPSWSSSP++PYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQTL 
Sbjct: 62  SHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLT 121

Query: 122 SMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIW 181
           SMDGT LTLYRTSC+SGLAAKYLARPDS VMVMVGAGAL PHLIKAHLAARPSV+KVIIW
Sbjct: 122 SMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIW 181

Query: 182 NRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAG 241
           NRTEE+AK LA+EM+KNAG++G+CFES+GNL+DAI+VADIVSCATNSE+PLVKG+KLKAG
Sbjct: 182 NRTEERAKVLADEMRKNAGMDGLCFESTGNLDDAISVADIVSCATNSEVPLVKGDKLKAG 241

Query: 242 AHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLVE 301
           A+L+LVGSF HSMRECDDLAIKRGRVFVD+E A+EEAGELVGA ERGVIEKR I+GNLV+
Sbjct: 242 AYLNLVGSFKHSMRECDDLAIKRGRVFVDSEEALEEAGELVGALERGVIEKRSILGNLVD 301

Query: 302 LIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           LIKGEVVGRR+ +EITVFKSVGSA+ DLV+AQ  YE +I
Sbjct: 302 LIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of MS000446 vs. NCBI nr
Match: XP_022922838.1 (protein SAR DEFICIENT 4 [Cucurbita moschata] >XP_022922839.1 protein SAR DEFICIENT 4 [Cucurbita moschata] >KAG7014743.1 lhpI, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543.1 bits (1398), Expect = 1.7e-150
Identity = 277/339 (81.71%), Postives = 313/339 (92.33%), Query Frame = 0

Query: 2   SSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMRQ 61
           +S PNNPKD+T+LT  +T R PMFI+KE LDSIL HQ LIEHF+SSL IVSPTISSPMRQ
Sbjct: 2   ASPPNNPKDKTSLTKKITGRVPMFITKEDLDSILPHQYLIEHFQSSLSIVSPTISSPMRQ 61

Query: 62  SHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLA 121
           SH + P+SSLLLMPSWSSSP++PYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQTL 
Sbjct: 62  SHTIQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLT 121

Query: 122 SMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIW 181
           S+DGT LTLYRTSC+SGLA KYLARPDS VMVMVGAGAL PHLIKAHLA RPSV+KVIIW
Sbjct: 122 SIDGTTLTLYRTSCISGLATKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVRKVIIW 181

Query: 182 NRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAG 241
           NRTEE+AK LA+EM+KNAG++G+CFES+GNL+DAI+VADIVSCATNSE+PLVKG+KLKAG
Sbjct: 182 NRTEERAKVLADEMRKNAGMDGLCFESTGNLDDAISVADIVSCATNSEVPLVKGDKLKAG 241

Query: 242 AHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLVE 301
           A+L+LVGSF HSMRECDDLAIKRGRVFVD+E A+EEAGELVGAFERGVIEKR I+GNLV+
Sbjct: 242 AYLNLVGSFKHSMRECDDLAIKRGRVFVDSEEALEEAGELVGAFERGVIEKRSILGNLVD 301

Query: 302 LIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           LIKGEVVGRR+ +EITVFKSVGSA+ DLV+AQ  YE +I
Sbjct: 302 LIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of MS000446 vs. NCBI nr
Match: XP_022984671.1 (protein SAR DEFICIENT 4-like [Cucurbita maxima] >XP_022984672.1 protein SAR DEFICIENT 4-like [Cucurbita maxima])

HSP 1 Score: 539.7 bits (1389), Expect = 1.9e-149
Identity = 277/339 (81.71%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 2   SSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMRQ 61
           +S  NNPKDET+LT  +T R PMFI+KE LDSIL HQNLIEHF+SSL IVSPTISSPMRQ
Sbjct: 2   ASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMRQ 61

Query: 62  SHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLA 121
           SH V P+SSLLLMPSWSSSP++PYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQTL 
Sbjct: 62  SHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLT 121

Query: 122 SMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIW 181
           SMDGT LTLYRTSC+SGLAAKYLARPDS VMVMVGAGAL PHLIKAHLAARPSV+KVIIW
Sbjct: 122 SMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIW 181

Query: 182 NRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAG 241
           NRTEE+AK LA+EM+KNAG++G+CFES+ NL+DAI++ADIVSCATNSE+PLVKG++LKAG
Sbjct: 182 NRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAG 241

Query: 242 AHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLVE 301
           A+L+LVGSF HSM ECDDLAIKRGRVFVD+E A+EEAGELVGA ERGVIEKR I+GNLV+
Sbjct: 242 AYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLVD 301

Query: 302 LIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           LIKGEVVGRR+ +EITVFKSVGSA+ DLV+AQ  YE +I
Sbjct: 302 LIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of MS000446 vs. ExPASy Swiss-Prot
Match: Q9FLY0 (Protein SAR DEFICIENT 4 OS=Arabidopsis thaliana OX=3702 GN=SARD4 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.5e-112
Identity = 208/316 (65.82%), Postives = 259/316 (81.96%), Query Frame = 0

Query: 23  PMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMRQSHAVHPNSSLLLMPSWSSSPS 82
           P+FI  E+  SILSH+ LI HFR++LP  S TI+SP+RQ++ V   SSLLLMPSWSSS S
Sbjct: 5   PVFIPAESFPSILSHETLINHFRTNLPKHSSTITSPVRQNYTVSSPSSLLLMPSWSSSSS 64

Query: 83  IPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLASMDGTALTLYRTSCVSGLAAK 142
           +PY+GVKLVT+FP NS+ NLP IH SY+LFSSTTGQTLA+MDGT LTLYRTS VSGL +K
Sbjct: 65  LPYMGVKLVTYFPHNSSQNLPGIHGSYTLFSSTTGQTLATMDGTVLTLYRTSSVSGLGSK 124

Query: 143 YLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIWNRTEEKAKKLAEEMQKNAGLE 202
            LAR DS V++MVG+GALAPHLIK+HLAA+PS+++VIIWNRT ++A++LAE + K+   +
Sbjct: 125 ILARDDSQVLIMVGSGALAPHLIKSHLAAKPSLRRVIIWNRTPQRAQELAETLSKDPQHK 184

Query: 203 GVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAGAHLDLVGSFSHSMRECDDLAI 262
            + F+S  +L+  I + DI+SCATNS +PLVKGE LK G HLDLVGSFSH M+ECDD AI
Sbjct: 185 EISFDSHDSLDQIIPLGDIISCATNSTVPLVKGEFLKPGTHLDLVGSFSHEMKECDDNAI 244

Query: 263 KRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLVELIKGEVVGRRDGNEITVFKSV 322
           +RG VFVDN+ A+ EAGEL GAFERGVI++ +I GNLVELIKG+  GR+   +ITVFKSV
Sbjct: 245 QRGSVFVDNDTAMIEAGELAGAFERGVIKREDICGNLVELIKGDKEGRKSSTDITVFKSV 304

Query: 323 GSAMVDLVSAQLVYET 339
           GS  VDL++AQLV+ET
Sbjct: 305 GSGTVDLLTAQLVHET 320

BLAST of MS000446 vs. ExPASy Swiss-Prot
Match: Q485R8 (Delta(1)-pyrroline-2-carboxylate reductase OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) OX=167879 GN=lhpI PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.0e-45
Identity = 118/312 (37.82%), Postives = 178/312 (57.05%), Query Frame = 0

Query: 37  HQNLIEHFRSSLPIV----SPTISSPMRQSHAVHPNSS-----LLLMPSWSSSPSIPYIG 96
           HQNL  +F   +P++    S   S P RQ +++ P  S       L+PSW+       IG
Sbjct: 10  HQNL--NFEELIPLLKQSFSRPFSMPQRQVYSLAPEQSENHDAFALLPSWNEE----VIG 69

Query: 97  VKLVTHFPQNS-AINLPAIHASYSLFSSTTGQTLASMDGTALTLYRTSCVSGLAAKYLAR 156
            K  T+FP N+   +LP + +   LF   TG+ LA +DGT++T +RT+ +S LA++ L+R
Sbjct: 70  NKAFTYFPDNAKKHDLPGLFSKIMLFKRQTGEPLALVDGTSVTYWRTAAISALASQLLSR 129

Query: 157 PDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIWNRTEEKAKKLAEEMQKNAGLEGVCF 216
            +S  +++ G G LA +L+KAHL  R  +K+V +W R  +K  KL  +   +     V F
Sbjct: 130 KNSQHLMLFGTGNLASYLVKAHLTVR-DIKQVTLWGRNAKKVSKLIADF--SILYPAVTF 189

Query: 217 ESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAGAHLDLVGSFSHSMRECDDLAIKRGR 276
           ++S ++   +A ADI+ CAT ++ PL  G  + AG H+D +G+     RECD   I R R
Sbjct: 190 KTSVDVNAEVASADIICCATGAKTPLFDGNSVSAGCHIDCLGNHMTDARECDTTTILRAR 249

Query: 277 VFVDN-EGAVEEAGELVGAFERGVIEKREIVGNLVELIKGEVVGRRDGNEITVFKSVGSA 336
           VFVD+    + EAGEL+         K EIVG L ++ K   + R+  +EIT+FKSVG+A
Sbjct: 250 VFVDSLTNTLNEAGELLIPMAEDAFNKDEIVGELADMCKTPSMLRQSSDEITLFKSVGTA 309

Query: 337 MVDLVSAQLVYE 338
           + DLV+A  V E
Sbjct: 310 ISDLVAAHSVVE 312

BLAST of MS000446 vs. ExPASy Swiss-Prot
Match: Q9HDZ0 (Uncharacterized protein P11E10.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP11E10.01 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 6.4e-44
Identity = 120/327 (36.70%), Postives = 180/327 (55.05%), Query Frame = 0

Query: 20  SRSPMFISKEALDSILSHQNLIEHFRSSLP--IVSPT-ISSPMRQSH-AVHPNSSLLLMP 79
           S S  F+S+E L S L    LI   R      +V PT +  P+ + + +   N+ LL+MP
Sbjct: 2   SGSCQFLSRETLSSSLGWIPLINALREIFTENVVCPTRLHYPIDEDNPSSTANNILLIMP 61

Query: 80  SWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLASMDGTALTLYRTSC 139
            W       ++GVK V  FP+N+   LP++ + Y L  +TTG  LA +DG  LT  RT+ 
Sbjct: 62  CWIPG---KFLGVKQVNVFPENTKHGLPSLSSHYLLSDATTGCHLAQLDGNELTSRRTAA 121

Query: 140 VSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIWNRTEEKAKKLAEEM 199
            S LA+ YL++ DS+ ++++G+G +A  LI AH + RP ++ V IWN   E AK LA   
Sbjct: 122 ASALASSYLSKEDSTSLLIIGSGKVAEKLIHAHCSVRP-IRSVRIWNHRFESAKSLASRA 181

Query: 200 QKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAGAHLDLVGSFSHSMR 259
            K      +      NLE+A+  +DI+S AT S+ P++ G  +  G H+DLVG F+  M 
Sbjct: 182 SKELPSIQIDAVPIQNLENAVKCSDIISSATLSKKPIIVGSWINPGTHIDLVGGFTPHMH 241

Query: 260 ECDDLAIKRGRVFVD-NEGAVEEAGELVGAFERGVIEKREIVGNLVELIKGEVVGR---- 319
           E D   I+   VFVD  +GA+ EAG+L+   + G+    E++ +L +L   +  GR    
Sbjct: 242 EADSKCIQISNVFVDTRKGALHEAGDLLTPIKEGLFSPNEVIADLFDLCNNKHSGRSQLK 301

Query: 320 RDGNEITVFKSVGSAMVDLVSAQLVYE 338
                IT+FKSVG +  DL +A L Y+
Sbjct: 302 NPAEAITLFKSVGDSREDLAAASLAYK 324

BLAST of MS000446 vs. ExPASy Swiss-Prot
Match: Q63FA5 (Delta(1)-pyrroline-2-carboxylate reductase OS=Bacillus cereus (strain ZK / E33L) OX=288681 GN=arcB PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 5.8e-37
Identity = 104/287 (36.24%), Postives = 162/287 (56.45%), Query Frame = 0

Query: 57  SPMRQS-HAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSST 116
           +P+R S    +  ++ L+MP  S +  +  +G+K+VT  P+N  I    I+    L    
Sbjct: 36  TPIRTSLPFANEQNTALIMP--SVAEGLEALGLKVVTVVPENKKIGKKTINGIVMLSDFQ 95

Query: 117 TGQTLASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSV 176
           TG+ LA ++G+ LT+ RT  +SG+A K+LAR ++  + ++G G  A  + +A  A R  +
Sbjct: 96  TGEPLALLEGSYLTMIRTGALSGVATKHLARHNAKTLCIIGTGEQAKGIAEAVFAVR-DI 155

Query: 177 KKVIIWNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKG 236
           +KV+++NRTEEKA   A+ +Q+  G     +    N  +AI+ ADI+   TN+  P V  
Sbjct: 156 EKVMLYNRTEEKAYAFAQYIQEKFGKPAYVY---ANANEAISEADIIVTTTNASTP-VFS 215

Query: 237 EKLKAGAHLDLVGSFSHSMRECDDLAIKRGR--VFVDNEGAVEEAGELVGAFERGVIEKR 296
           EKL+ G H++ VGSF  +M+E    AI      V    E A+EE G+L      G+ E  
Sbjct: 216 EKLQKGVHINAVGSFRPNMQELPSHAIANANKVVVESKEAALEETGDLQVPVREGLFEAS 275

Query: 297 EIVGNLVELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           +I   L ++I GE  GR    EITVFKSVG A+VD++ A+ +YE  +
Sbjct: 276 DIHAELGQIISGEKAGRESDEEITVFKSVGLAVVDIIVAKYLYEKAV 315

BLAST of MS000446 vs. ExPASy Swiss-Prot
Match: Q73CR9 (Delta(1)-pyrroline-2-carboxylate reductase OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=BCE_0995 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.5e-36
Identity = 108/321 (33.64%), Postives = 183/321 (57.01%), Query Frame = 0

Query: 24  MFISKEALDSILSHQNLIEHFRSSLPIVSPTIS-SPMRQS-HAVHPNSSLLLMPSWSSSP 83
           + IS     ++++   +IE+   +L   S   + +P+R S    +  ++ L+MP  S + 
Sbjct: 2   LVISANEQRNLVNMNEVIEYAALALKEFSAERTITPIRDSLPFANEQNTALIMP--SVAE 61

Query: 84  SIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLASMDGTALTLYRTSCVSGLAA 143
            +  +G+K+VT  P+N  I    I+    L    TG+ LA ++G+ LT+ RT  +SG+A 
Sbjct: 62  GLEALGLKVVTVVPENKKIGKKTINGIVMLSDFQTGEPLALLEGSYLTMIRTGALSGVAT 121

Query: 144 KYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIWNRTEEKAKKLAEEMQKNAGL 203
           K+LAR ++  + ++G G  A  + +A  A R  ++KVI++NRTEEKA   ++ +Q+    
Sbjct: 122 KHLARHNAKTLCIIGTGEQAKGIAEAVFAVR-DIEKVILYNRTEEKAYAFSQYIQEKFNK 181

Query: 204 EGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAGAHLDLVGSFSHSMRECDDLA 263
               + S+    +AI+ ADI++  TN+  P V  +KL+ G H++ VGSF  SM+E    A
Sbjct: 182 PAYVYTSA---NEAISEADIIATTTNASTP-VFSKKLQKGVHVNAVGSFRPSMQELPSHA 241

Query: 264 IKRG-RVFVDN-EGAVEEAGELVGAFERGVIEKREIVGNLVELIKGEVVGRRDGNEITVF 323
           I    +V V++ E A+EE G+L    + G+ +  +I   L ++I GE  GR    E+TVF
Sbjct: 242 IANATKVVVESKEAALEETGDLQVPIQEGLFKSSDIHAELGQIISGEKAGRESDEEVTVF 301

Query: 324 KSVGSAMVDLVSAQLVYETTI 341
           KSVG A+VD++ A+ +YE  +
Sbjct: 302 KSVGLAVVDIIVAKYLYERAV 315

BLAST of MS000446 vs. ExPASy TrEMBL
Match: A0A6J1CIG8 (uncharacterized protein LOC111011673 OS=Momordica charantia OX=3673 GN=LOC111011673 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.8e-181
Identity = 339/340 (99.71%), Postives = 339/340 (99.71%), Query Frame = 0

Query: 1   ASSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMR 60
           ASSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMR
Sbjct: 2   ASSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMR 61

Query: 61  QSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTL 120
           QSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTL
Sbjct: 62  QSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTL 121

Query: 121 ASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVII 180
           ASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVII
Sbjct: 122 ASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVII 181

Query: 181 WNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKA 240
           WNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIV CATNSEIPLVKGEKLKA
Sbjct: 182 WNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVCCATNSEIPLVKGEKLKA 241

Query: 241 GAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLV 300
           GAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLV
Sbjct: 242 GAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLV 301

Query: 301 ELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           ELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI
Sbjct: 302 ELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341

BLAST of MS000446 vs. ExPASy TrEMBL
Match: A0A6J1E7X4 (protein SAR DEFICIENT 4 OS=Cucurbita moschata OX=3662 GN=LOC111430702 PE=4 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 8.2e-151
Identity = 277/339 (81.71%), Postives = 313/339 (92.33%), Query Frame = 0

Query: 2   SSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMRQ 61
           +S PNNPKD+T+LT  +T R PMFI+KE LDSIL HQ LIEHF+SSL IVSPTISSPMRQ
Sbjct: 2   ASPPNNPKDKTSLTKKITGRVPMFITKEDLDSILPHQYLIEHFQSSLSIVSPTISSPMRQ 61

Query: 62  SHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLA 121
           SH + P+SSLLLMPSWSSSP++PYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQTL 
Sbjct: 62  SHTIQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLT 121

Query: 122 SMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIW 181
           S+DGT LTLYRTSC+SGLA KYLARPDS VMVMVGAGAL PHLIKAHLA RPSV+KVIIW
Sbjct: 122 SIDGTTLTLYRTSCISGLATKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVRKVIIW 181

Query: 182 NRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAG 241
           NRTEE+AK LA+EM+KNAG++G+CFES+GNL+DAI+VADIVSCATNSE+PLVKG+KLKAG
Sbjct: 182 NRTEERAKVLADEMRKNAGMDGLCFESTGNLDDAISVADIVSCATNSEVPLVKGDKLKAG 241

Query: 242 AHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLVE 301
           A+L+LVGSF HSMRECDDLAIKRGRVFVD+E A+EEAGELVGAFERGVIEKR I+GNLV+
Sbjct: 242 AYLNLVGSFKHSMRECDDLAIKRGRVFVDSEEALEEAGELVGAFERGVIEKRSILGNLVD 301

Query: 302 LIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           LIKGEVVGRR+ +EITVFKSVGSA+ DLV+AQ  YE +I
Sbjct: 302 LIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of MS000446 vs. ExPASy TrEMBL
Match: A0A6J1J2T7 (protein SAR DEFICIENT 4-like OS=Cucurbita maxima OX=3661 GN=LOC111482883 PE=4 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 9.1e-150
Identity = 277/339 (81.71%), Postives = 312/339 (92.04%), Query Frame = 0

Query: 2   SSTPNNPKDETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMRQ 61
           +S  NNPKDET+LT  +T R PMFI+KE LDSIL HQNLIEHF+SSL IVSPTISSPMRQ
Sbjct: 2   ASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMRQ 61

Query: 62  SHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLA 121
           SH V P+SSLLLMPSWSSSP++PYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQTL 
Sbjct: 62  SHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLT 121

Query: 122 SMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIW 181
           SMDGT LTLYRTSC+SGLAAKYLARPDS VMVMVGAGAL PHLIKAHLAARPSV+KVIIW
Sbjct: 122 SMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIW 181

Query: 182 NRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAG 241
           NRTEE+AK LA+EM+KNAG++G+CFES+ NL+DAI++ADIVSCATNSE+PLVKG++LKAG
Sbjct: 182 NRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAG 241

Query: 242 AHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLVE 301
           A+L+LVGSF HSM ECDDLAIKRGRVFVD+E A+EEAGELVGA ERGVIEKR I+GNLV+
Sbjct: 242 AYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLVD 301

Query: 302 LIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           LIKGEVVGRR+ +EITVFKSVGSA+ DLV+AQ  YE +I
Sbjct: 302 LIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of MS000446 vs. ExPASy TrEMBL
Match: A0A0A0L8U0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G184010 PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.2e-148
Identity = 283/341 (82.99%), Postives = 313/341 (91.79%), Query Frame = 0

Query: 2   SSTPNNPKD--ETNLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPM 61
           +S  NNPKD  ETNLT  +T R+PM I+KEALDSIL HQNLIEHF+SSLPIV PTISSPM
Sbjct: 2   ASASNNPKDDTETNLTKKITVRTPMVITKEALDSILQHQNLIEHFQSSLPIVFPTISSPM 61

Query: 62  RQSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQT 121
           RQSHAV P+SSLLLMPSWSSSPS+PYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQT
Sbjct: 62  RQSHAVQPHSSLLLMPSWSSSPSLPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQT 121

Query: 122 LASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVI 181
           L SMDGTALTLYRTSC+SGLAAKYLARPDS VMVMVGAGAL PHLIKAHLA RPSVKKVI
Sbjct: 122 LGSMDGTALTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVKKVI 181

Query: 182 IWNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLK 241
           IWNRTEE+AKKLA+EM++NAGL+GVCFESS NL+DAI+VADIVSCATNSE+PLVKG+KLK
Sbjct: 182 IWNRTEERAKKLADEMRENAGLDGVCFESSANLDDAISVADIVSCATNSEVPLVKGDKLK 241

Query: 242 AGAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNL 301
            GA+L+LVGSF HSMRECDD+A+KRGRVFVD E A+EEAGELVGA ERGVIEK +I+G L
Sbjct: 242 GGAYLNLVGSFQHSMRECDDMAMKRGRVFVDCEEALEEAGELVGALERGVIEKSDIMG-L 301

Query: 302 VELI-KGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETT 340
           V+LI KGE+VGRR+ +EI VFKSVGSA+ DLV+AQL YETT
Sbjct: 302 VDLIKKGEIVGRRNEDEIFVFKSVGSAVFDLVAAQLAYETT 341

BLAST of MS000446 vs. ExPASy TrEMBL
Match: A0A5A7SWS6 (Delta(1)-pyrroline-2-carboxylate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001900 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.5e-147
Identity = 279/341 (81.82%), Postives = 312/341 (91.50%), Query Frame = 0

Query: 2   SSTPNNPKDET--NLTTNVTSRSPMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPM 61
           +ST NN KD+T  NLT  +T R+PM I+KEALDSIL HQNLIEHF+SSLPIV PTISSPM
Sbjct: 2   ASTSNNLKDDTKSNLTKKITVRTPMVITKEALDSILQHQNLIEHFQSSLPIVFPTISSPM 61

Query: 62  RQSHAVHPNSSLLLMPSWSSSPSIPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQT 121
           RQSH V P+SSLLLMPSWSSSPS+PYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQT
Sbjct: 62  RQSHVVQPHSSLLLMPSWSSSPSLPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQT 121

Query: 122 LASMDGTALTLYRTSCVSGLAAKYLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVI 181
           L SMDGTALTLYRTSC+SGLAAKYLARPDS VMVMVGAGAL PHLIKAHLA RPSVKKVI
Sbjct: 122 LGSMDGTALTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVKKVI 181

Query: 182 IWNRTEEKAKKLAEEMQKNAGLEGVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLK 241
           IWNRTEE+AK+LA+EM++NAGL+GVCFESS NL+DAI+VADIVSCATNSE+PLVKG+KLK
Sbjct: 182 IWNRTEERAKRLADEMRENAGLDGVCFESSANLDDAISVADIVSCATNSEVPLVKGDKLK 241

Query: 242 AGAHLDLVGSFSHSMRECDDLAIKRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNL 301
            GA+L+LVGSF  SMRECDD+AIKRGRVFVD E A+EEAGEL+GA ERGVIEK +I+G L
Sbjct: 242 CGAYLNLVGSFKPSMRECDDMAIKRGRVFVDCEEALEEAGELLGALERGVIEKSDILG-L 301

Query: 302 VELIKGEVVGRRDGNEITVFKSVGSAMVDLVSAQLVYETTI 341
           V+LIKG++VGRR+ +EI+VFKSVGSAM DLV+AQ  YETTI
Sbjct: 302 VDLIKGQIVGRRNEDEISVFKSVGSAMFDLVAAQFAYETTI 341

BLAST of MS000446 vs. TAIR 10
Match: AT5G52810.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 406.8 bits (1044), Expect = 1.8e-113
Identity = 208/316 (65.82%), Postives = 259/316 (81.96%), Query Frame = 0

Query: 23  PMFISKEALDSILSHQNLIEHFRSSLPIVSPTISSPMRQSHAVHPNSSLLLMPSWSSSPS 82
           P+FI  E+  SILSH+ LI HFR++LP  S TI+SP+RQ++ V   SSLLLMPSWSSS S
Sbjct: 5   PVFIPAESFPSILSHETLINHFRTNLPKHSSTITSPVRQNYTVSSPSSLLLMPSWSSSSS 64

Query: 83  IPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQTLASMDGTALTLYRTSCVSGLAAK 142
           +PY+GVKLVT+FP NS+ NLP IH SY+LFSSTTGQTLA+MDGT LTLYRTS VSGL +K
Sbjct: 65  LPYMGVKLVTYFPHNSSQNLPGIHGSYTLFSSTTGQTLATMDGTVLTLYRTSSVSGLGSK 124

Query: 143 YLARPDSSVMVMVGAGALAPHLIKAHLAARPSVKKVIIWNRTEEKAKKLAEEMQKNAGLE 202
            LAR DS V++MVG+GALAPHLIK+HLAA+PS+++VIIWNRT ++A++LAE + K+   +
Sbjct: 125 ILARDDSQVLIMVGSGALAPHLIKSHLAAKPSLRRVIIWNRTPQRAQELAETLSKDPQHK 184

Query: 203 GVCFESSGNLEDAIAVADIVSCATNSEIPLVKGEKLKAGAHLDLVGSFSHSMRECDDLAI 262
            + F+S  +L+  I + DI+SCATNS +PLVKGE LK G HLDLVGSFSH M+ECDD AI
Sbjct: 185 EISFDSHDSLDQIIPLGDIISCATNSTVPLVKGEFLKPGTHLDLVGSFSHEMKECDDNAI 244

Query: 263 KRGRVFVDNEGAVEEAGELVGAFERGVIEKREIVGNLVELIKGEVVGRRDGNEITVFKSV 322
           +RG VFVDN+ A+ EAGEL GAFERGVI++ +I GNLVELIKG+  GR+   +ITVFKSV
Sbjct: 245 QRGSVFVDNDTAMIEAGELAGAFERGVIKREDICGNLVELIKGDKEGRKSSTDITVFKSV 304

Query: 323 GSAMVDLVSAQLVYET 339
           GS  VDL++AQLV+ET
Sbjct: 305 GSGTVDLLTAQLVHET 320