MS000435 (gene) Bitter gourd (TR) v1

Overview


Name	MS000435
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	beta-carotene hydroxylase 2, chloroplastic
Location	scaffold44: 1663999 .. 1666161 (-)
RNA-Seq Expression	MS000435
Synteny	MS000435

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCGGCCTCTCCGCCGCCGTAGCGCCCAAACCATCCCATCTCTTCCTCACTACTTCCCACCTCTCCCCTAAACCTCGAGCTCCATTTCTGCTCCCCCCTGCAGTCTTCCGGAACAGTAAAATCCAATGGAAGATTCGGAGAAAATCGCTGTTCACCGTCTGCGTGCTCGTCGAGGATCAAAATAGTTCCGGCGAGATGGAGAATCTCGCCGATGAAGGATCGCCGATGATTGTGGTCCCTCAGATCCCGTCTCCTCGTGTTGCAGAAAAATTGGCTCGGAAGAGATCGGAGCGCTTTACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCCGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGTGCTCATATCACTATCTCTCTCTATCTTGAAACTTAAATTGTTTTTGTTTCCATTGGCGCCGTCTTCTTTCTGACTGAATACTGAATTGCGATTGCTTAATTTGGCGGTGTGCAGGGCGGAGAGATTTCTTTCTCTGAGATGTTTGGTACATTTTCTCTCTCCGTTGGCGCTGCTGTATGTTTTCGATTTCGTTTCTTCTACCTCTCTACTTTTTCCCGAAGAATTCAATTCGATTTGCCGTTGCCTAATTTTTCGCAATCAATTTGAGATTATCAGGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTTTCTAAACCTTCTTCTCTATATTATTATTTCGTAATTTGTGTTTGTCTGCTGAGAAAATTTCAAACAACAATCCACTGTGAAGCTTAATTTTTCTCATTTTCCGTCTTCACTTTCCTACCAAACAAACGGTTGAAAAAGAAATCGCTGACGCTTGGAGTACCTAAAACCTTCTTATCTATAGAATTCCGTTGTGTTTGGCCGCTGAGAAAATTTAAACAACAATTCACTGTGAAACTTTCTCTCATTTTCCATGTTCACTTTCCTAGCAAACAAACAACATTTAAGAAAAAACGATTGGTTGACGCTTGGACTTTGTTGATTTCCGCAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAGCTGAACGATGTTTTCGCCATTACCAACGCCGTGCCCGCCATAGCTCTCCTCTCTTATGGCTTCTTCCACAAAGGCCTCGTCCCCGGCCTCTGCTTCGGCGCTGTAAGTAAAATTTCTCTCTCATTATCTTTCAGAATCTCAGTTTGTTCATATTCCAACTGTTTAATGGTTATTTAATGACTGGGGATTAGTGCTAATGACGGTGTAAAATGACGAAAATAGCCCTGGAAAATGTGGTTTTTGGGTTACTGACACGCTGGCGGGTTGTGGCAGGGCCTCGGAATTACGGTCTTCGGCATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAGGGTCGCTGCCGCTCACCAGGTAAATTAATTAAACTTTTCAAAACACAATAATAATAATATCCAAAAATCTTTTTTTTATTTACTTAAAATAAAAATTATAATTTAAATATTTTTCATGTCTGAACCTCTGCTAATATATATGTAATAAACGGGTTTAAATTTAAGTTTTAAGAAAATCAGAAAATGTAGAGATCAACATGAATAGTTAAAAGCTTAGAAACAAAAATATGATTTTAAATTAATCTGTTAATTCAATATAAGTAATAGTTGTAATAAGAAAATAGCTACGGAGTTAAAAAATATAATAGTATTCTTAGACTTTGGACAAGCATATAGAAAAGTTATTTACGTGTCTGTGTAAATCTAAATTTTAAATATTGTCCACGATTTAAGTACTAAATTTTGGTGATTTTTTTTTTTTTTGGGTTTGGCCTTAGCTTCATCATTCGGATAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCAAAGGTCAGTCTATGATCTATTTATCTATTTGTTTTATTGCGAATTTTAAAATTTTTATTATTAACTATTTTCGAAATTTAAATGGTGTGGTGCAGGAATTAGAGGAGGTTGGAGGCATGGAAGAATTGGAGAAGGAAATCAGCAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCA

mRNA sequence

ATGGCGGCCGGCCTCTCCGCCGCCGTAGCGCCCAAACCATCCCATCTCTTCCTCACTACTTCCCACCTCTCCCCTAAACCTCGAGCTCCATTTCTGCTCCCCCCTGCAGTCTTCCGGAACAGTAAAATCCAATGGAAGATTCGGAGAAAATCGCTGTTCACCGTCTGCGTGCTCGTCGAGGATCAAAATAGTTCCGGCGAGATGGAGAATCTCGCCGATGAAGGATCGCCGATGATTGTGGTCCCTCAGATCCCGTCTCCTCGTGTTGCAGAAAAATTGGCTCGGAAGAGATCGGAGCGCTTTACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCCGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTTCTTTCTCTGAGATGTTTGGTACATTTTCTCTCTCCGTTGGCGCTGCTGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAGCTGAACGATGTTTTCGCCATTACCAACGCCGTGCCCGCCATAGCTCTCCTCTCTTATGGCTTCTTCCACAAAGGCCTCGTCCCCGGCCTCTGCTTCGGCGCTGGCCTCGGAATTACGGTCTTCGGCATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAGGGTCGCTGCCGCTCACCAGCTTCATCATTCGGATAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCAAAGGAATTAGAGGAGGTTGGAGGCATGGAAGAATTGGAGAAGGAAATCAGCAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCA

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCGTAGCGCCCAAACCATCCCATCTCTTCCTCACTACTTCCCACCTCTCCCCTAAACCTCGAGCTCCATTTCTGCTCCCCCCTGCAGTCTTCCGGAACAGTAAAATCCAATGGAAGATTCGGAGAAAATCGCTGTTCACCGTCTGCGTGCTCGTCGAGGATCAAAATAGTTCCGGCGAGATGGAGAATCTCGCCGATGAAGGATCGCCGATGATTGTGGTCCCTCAGATCCCGTCTCCTCGTGTTGCAGAAAAATTGGCTCGGAAGAGATCGGAGCGCTTTACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCCGTCATGGCGGTTTATTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTTCTTTCTCTGAGATGTTTGGTACATTTTCTCTCTCCGTTGGCGCTGCTGTCGGCATGGAGTTTTGGGCGAGATGGGCTCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAGCTGAACGATGTTTTCGCCATTACCAACGCCGTGCCCGCCATAGCTCTCCTCTCTTATGGCTTCTTCCACAAAGGCCTCGTCCCCGGCCTCTGCTTCGGCGCTGGCCTCGGAATTACGGTCTTCGGCATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAGGGTCGCTGCCGCTCACCAGCTTCATCATTCGGATAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCAAAGGAATTAGAGGAGGTTGGAGGCATGGAAGAATTGGAGAAGGAAATCAGCAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCA

Protein sequence

MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVEDQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRIKSTATKSINGS

Homology

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BLAST of MS000435 vs. NCBI nr
Match: XP_022141331.1 (beta-carotene hydroxylase 2, chloroplastic [Momordica charantia])

HSP 1 Score: 623.6 bits (1607), Expect = 9.0e-175
Identity = 308/311 (99.04%), Postives = 310/311 (99.68%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLSAAVAPKPSHLFLTTSHLS KPRAPFLLPPAVFRNSKIQWK+RRKSLFTVCVLVE
Sbjct: 1   MAAGLSAAVAPKPSHLFLTTSHLSLKPRAPFLLPPAVFRNSKIQWKVRRKSLFTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAITNA+PAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAITNALPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI
Sbjct: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300

Query: 301 KSTATKSINGS 312
           KSTATKSINGS
Sbjct: 301 KSTATKSINGS 311

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BLAST of MS000435 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 574.7 bits (1480), Expect = 4.8e-160
Identity = 280/311 (90.03%), Postives = 298/311 (95.82%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLSAA+ PKP HL LT+SHLSPKPR PFL PP+VFRNS+IQWK+RR++LFTVCVLVE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQNSSGE+ENL+DE SP I+VPQIPSP V+E+LARK+SERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQNSSGEVENLSDEVSP-IIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEI FSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG+EELEKEI+RRI
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRI 300

Query: 301 KSTATKSINGS 312
           K  A KSI+GS
Sbjct: 301 KWAAPKSIDGS 310

[Close]

BLAST of MS000435 vs. NCBI nr
Match: KAG7014750.1 (Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.9 bits (1470), Expect = 6.9e-159
Identity = 281/311 (90.35%), Postives = 295/311 (94.86%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAA LSAA+ PKP HLFL +SHLSPKPR PFL PP+VFRNS+IQ KIRRK+LFTVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSPKPRTPFLFPPSVFRNSRIQRKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQN+SGE+E+L+DE  P IVVPQIPSP VA++LARKRSERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQNNSGEVESLSDE-EPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEI FSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEI+RRI
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRI 300

Query: 301 KSTATKSINGS 312
           K T TKSINGS
Sbjct: 301 KLTTTKSINGS 310

[Close]

BLAST of MS000435 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 569.7 bits (1467), Expect = 1.5e-158
Identity = 279/311 (89.71%), Postives = 296/311 (95.18%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLSA + PKP H+FLT+S+LSPKPR PFL P +VFRNS+IQWK+RRK+L TVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQ+SS E+ENLADE  P I+VPQIPSP VAEKLARK+SERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQSSSSEVENLADEKLP-IIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRF+WQMEGGEI FSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP
Sbjct: 121 AVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG+EELEKEISRRI
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRI 300

Query: 301 KSTATKSINGS 312
           KSTA K INGS
Sbjct: 301 KSTAKKPINGS 310

[Close]

BLAST of MS000435 vs. NCBI nr
Match: XP_004140758.1 (beta-carotene hydroxylase 2, chloroplastic [Cucumis sativus] >KGN57410.1 hypothetical protein Csa_009664 [Cucumis sativus])

HSP 1 Score: 567.4 bits (1461), Expect = 7.7e-158
Identity = 276/311 (88.75%), Postives = 296/311 (95.18%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLSAA  PKP HLFLT+SHLSPKP +PFL PP+VFRNS+ QWK+RRK+L TVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
            QNSSG+++NL+DE SP IVVPQIPSP V+E+LARK+SERFTYLVAAVMS+FGITSMAVM
Sbjct: 61  GQNSSGKVDNLSDEESP-IVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEI FSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELND+FAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG+EELEKEI+RRI
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRI 300

Query: 301 KSTATKSINGS 312
           K TA KSI+GS
Sbjct: 301 KWTARKSIDGS 310

[Close]

BLAST of MS000435 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 438.3 bits (1126), Expect = 7.0e-122
Identity = 218/288 (75.69%), Postives = 240/288 (83.33%), Query Frame = 0


Query: 25  PKPRAPFLLPPAVFR-NSKIQWKIRRKSLFTVCVLVEDQNSSGEMENLADEGSPMIVVPQ 84
           P P  P + P   F  N     + RR+  FTVC ++ED     + E   +E   M +  Q
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFE-AGEEDIEMKIEEQ 86

Query: 85  IPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEISFSEMFGT 144
           I + R+AEKLARK+SERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+ FSEMFGT
Sbjct: 87  ISATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGT 146

Query: 145 FSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAITNAVPAIALL 204
           F+LSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREGPFELNDVFAI NAVPAIALL
Sbjct: 147 FALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 206

Query: 205 SYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRRVAAAHQL 264
            YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGP+ANVPY R+VAAAH L
Sbjct: 207 DYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSL 266

Query: 265 HHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRIKSTATKSINGS 312
           HHS+KFNGVPYGLFLGPKELEEVGG+EELEKE++RR     T+ I GS
Sbjct: 267 HHSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRR-----TRYIKGS 308

[Close]

BLAST of MS000435 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.4e-116
Identity = 223/316 (70.57%), Postives = 250/316 (79.11%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLS AV  KP H   ++S    + R P  L       S     +R K  F+VC +VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSL-------SGFSPSLRFKR-FSVCYVVE 60

Query: 61  DQNSSGEMENLADE-----GSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGIT 120
           ++  +  +EN  DE      S   +  +  + R+AEKL RK+SER TYL+AA++SSFGIT
Sbjct: 61  ERRQNSPIEN--DERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGIT 120

Query: 121 SMAVMAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHE 180
           SMAVMAVYYRF WQMEGGEIS  EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHE
Sbjct: 121 SMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHE 180

Query: 181 SHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVH 240
           SHHKPREGPFELNDVFAI NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVH
Sbjct: 181 SHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVH 240

Query: 241 DGLVHKRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKE 300
           DGLVHKRFPVGPIA+VPY R+VAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+KE
Sbjct: 241 DGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKE 300

Query: 301 ISRRIKSTATKSINGS 312
           ISRRIKS    S +GS
Sbjct: 301 ISRRIKSYKKASGSGS 306

[Close]

BLAST of MS000435 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 3.2e-114
Identity = 212/304 (69.74%), Postives = 241/304 (79.28%), Query Frame = 0


Query: 6   SAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVEDQNSS 65
           S+++ PK S +  +T  L   P  PF L     R        R K   TVC ++E++   
Sbjct: 23  SSSLTPKSSPVSTSTPTLVVFP--PFKLVSKSLRT-------RSKPRLTVCFVLEEKELR 82

Query: 66  GEMENLADEGSPMIVVPQ-------IPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMA 125
           G++   +D+      V +         + ++A+KLARK+SERFTYLVAAVMSSFGITSMA
Sbjct: 83  GKLVVASDDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMA 142

Query: 126 VMAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHH 185
           V++VYYRF WQMEGGEI  SEMFGTF+LSVGAAVGMEFWARWAH ALWHASLWHMHESHH
Sbjct: 143 VLSVYYRFSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHH 202

Query: 186 KPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGL 245
           KPREGPFELND+FAI NAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGL
Sbjct: 203 KPREGPFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGL 262

Query: 246 VHKRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISR 303
           VHKRFPVGPIA+VPYFRRVAAAH LHHSDKFNGVPYGLFLGPKELEEVGG++ LE EI+R
Sbjct: 263 VHKRFPVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINR 317

[Close]

BLAST of MS000435 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.6e-113
Identity = 210/302 (69.54%), Postives = 241/302 (79.80%), Query Frame = 0


Query: 1   MAAGLSA-AVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLV 60
           MAAGLS  AV  KP +    +S  +  P +  + PP++  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAV 120
           E++  S  M++     S       + + R+ +K  +K+SERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
           MAVYYRF WQM+GGE+S  EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAITNAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRR 300
           HKRFPVGPIANVPY R+VAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEISRR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

Query: 301 IK 302
           IK
Sbjct: 301 IK 294

[Close]

BLAST of MS000435 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 403.3 bits (1035), Expect = 2.5e-111
Identity = 202/291 (69.42%), Postives = 231/291 (79.38%), Query Frame = 0


Query: 25  PKPRAPFLLPPAVF-----RNSKIQWKIRRKSLFTVCVLVED-------QNSSGEMENLA 84
           P P+     PP +F      N     + RRK     C +++D            + E + 
Sbjct: 22  PAPKYFATAPPLLFFSPLTCNLDAILRSRRKPRLAACFVLKDDKLYTAQSGKQSDTEAIG 81

Query: 85  DEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEG 144
           DE        +  + R+AEK ARK+SERFTYLVAAVMSS GITSMAV++VYYRF WQMEG
Sbjct: 82  DEIEVETNEEKSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEG 141

Query: 145 GEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFA 204
           GE+ FSEMF TF+L+ GAA+GME+WARWAHRALWHASLWHMHESHH+PREGPFELND+FA
Sbjct: 142 GEMPFSEMFCTFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFA 201

Query: 205 ITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP 264
           I NAVPAIALLS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP
Sbjct: 202 IINAVPAIALLSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVP 261

Query: 265 YFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRIKST 304
           YF+RVAAAHQLHHSDKF+GVPYGLFLGPKELEEVG +EELEKE++RRIKS+
Sbjct: 262 YFQRVAAAHQLHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIKSS 312

[Close]

BLAST of MS000435 vs. ExPASy TrEMBL
Match: A0A6J1CIV1 (beta-carotene hydroxylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111011750 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 4.4e-175
Identity = 308/311 (99.04%), Postives = 310/311 (99.68%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLSAAVAPKPSHLFLTTSHLS KPRAPFLLPPAVFRNSKIQWK+RRKSLFTVCVLVE
Sbjct: 1   MAAGLSAAVAPKPSHLFLTTSHLSLKPRAPFLLPPAVFRNSKIQWKVRRKSLFTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAITNA+PAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAITNALPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI
Sbjct: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300

Query: 301 KSTATKSINGS 312
           KSTATKSINGS
Sbjct: 301 KSTATKSINGS 311

[Close]

BLAST of MS000435 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.7e-158
Identity = 276/311 (88.75%), Postives = 296/311 (95.18%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLSAA  PKP HLFLT+SHLSPKP +PFL PP+VFRNS+ QWK+RRK+L TVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
            QNSSG+++NL+DE SP IVVPQIPSP V+E+LARK+SERFTYLVAAVMS+FGITSMAVM
Sbjct: 61  GQNSSGKVDNLSDEESP-IVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEI FSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELND+FAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG+EELEKEI+RRI
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRI 300

Query: 301 KSTATKSINGS 312
           K TA KSI+GS
Sbjct: 301 KWTARKSIDGS 310

[Close]

BLAST of MS000435 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.3e-158
Identity = 279/311 (89.71%), Postives = 293/311 (94.21%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAA LSAA+ PKP HLFL +SHLS KPR PFL PP+VFRNS+IQ K+RRK+LFTVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQNSSGE+ENL+DE  P IVVPQIPSP VA++LARKRSERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQNSSGEVENLSDE-EPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEI FSEMFGTF LSVGAAVGMEFWARWAHRALWH+S+WHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEI+RRI
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRI 300

Query: 301 KSTATKSINGS 312
           K T TKSINGS
Sbjct: 301 KLTTTKSINGS 310

[Close]

BLAST of MS000435 vs. ExPASy TrEMBL
Match: A0A6J1E4L5 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430578 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 6.3e-158
Identity = 278/311 (89.39%), Postives = 294/311 (94.53%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAA +S A+ P+P HLFL +SHLSPKPR PFL PP+VFRNS+IQ KIRRK+LFTVCVLVE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQNSSGE+E+L+DE  P IVVPQIPSP VA++LARKRSERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQNSSGEVESLSDE-EPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRFYWQMEGGEI FSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKP
Sbjct: 121 AVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEI+RRI
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRI 300

Query: 301 KSTATKSINGS 312
           K T TKSI+GS
Sbjct: 301 KLTTTKSIDGS 310

[Close]

BLAST of MS000435 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.4e-157
Identity = 276/311 (88.75%), Postives = 295/311 (94.86%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLSA + PKP H+FLT+S+LSPKPR PFL P +VFRNS+IQWK+RRK+L TVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAVM 120
           DQ+SS E+ENLADE  P I+VPQIPSP VAEKLARK+SERFTYLVAAVMSSFGITSMAVM
Sbjct: 61  DQSSSSEVENLADEKLP-IIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVM 120

Query: 121 AVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180
           AVYYRF+WQMEGGEI FSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP
Sbjct: 121 AVYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKP 180

Query: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240
           REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH
Sbjct: 181 REGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVH 240

Query: 241 KRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRRI 300
           KRFPVGPIANVPYFR+VAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG++ELEKEI+RR 
Sbjct: 241 KRFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRT 300

Query: 301 KSTATKSINGS 312
           KSTA K INGS
Sbjct: 301 KSTAKKPINGS 310

[Close]

BLAST of MS000435 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 419.1 bits (1076), Expect = 3.1e-117
Identity = 223/316 (70.57%), Postives = 250/316 (79.11%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLS AV  KP H   ++S    + R P  L       S     +R K  F+VC +VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSL-------SGFSPSLRFKR-FSVCYVVE 60

Query: 61  DQNSSGEMENLADE-----GSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGIT 120
           ++  +  +EN  DE      S   +  +  + R+AEKL RK+SER TYL+AA++SSFGIT
Sbjct: 61  ERRQNSPIEN--DERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGIT 120

Query: 121 SMAVMAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHE 180
           SMAVMAVYYRF WQMEGGEIS  EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHE
Sbjct: 121 SMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHE 180

Query: 181 SHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVH 240
           SHHKPREGPFELNDVFAI NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVH
Sbjct: 181 SHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVH 240

Query: 241 DGLVHKRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKE 300
           DGLVHKRFPVGPIA+VPY R+VAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+KE
Sbjct: 241 DGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKE 300

Query: 301 ISRRIKSTATKSINGS 312
           ISRRIKS    S +GS
Sbjct: 301 ISRRIKSYKKASGSGS 306

[Close]

BLAST of MS000435 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 409.1 bits (1050), Expect = 3.3e-114
Identity = 210/302 (69.54%), Postives = 241/302 (79.80%), Query Frame = 0


Query: 1   MAAGLSA-AVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLV 60
           MAAGLS  AV  KP +    +S  +  P +  + PP++  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAV 120
           E++  S  M++     S       + + R+ +K  +K+SERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
           MAVYYRF WQM+GGE+S  EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAITNAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRR 300
           HKRFPVGPIANVPY R+VAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEISRR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

Query: 301 IK 302
           IK
Sbjct: 301 IK 294

[Close]

BLAST of MS000435 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 261.5 bits (667), Expect = 8.4e-70
Identity = 145/225 (64.44%), Postives = 166/225 (73.78%), Query Frame = 0


Query: 1   MAAGLSAAVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLVE 60
           MAAGLS AV  KP H   ++S    + R P  L       S     +R K  F+VC +VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSL-------SGFSPSLRFKR-FSVCYVVE 60

Query: 61  DQNSSGEMENLADE-----GSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGIT 120
           ++  +  +EN  DE      S   +  +  + R+AEKL RK+SER TYL+AA++SSFGIT
Sbjct: 61  ERRQNSPIEN--DERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGIT 120

Query: 121 SMAVMAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHE 180
           SMAVMAVYYRF WQMEGGEIS  EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHE
Sbjct: 121 SMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHE 180

Query: 181 SHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGA 221
           SHHKPREGPFELNDVFAI NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 SHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of MS000435 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 245.7 bits (626), Expect = 4.8e-65
Identity = 148/302 (49.01%), Postives = 177/302 (58.61%), Query Frame = 0


Query: 1   MAAGLSA-AVAPKPSHLFLTTSHLSPKPRAPFLLPPAVFRNSKIQWKIRRKSLFTVCVLV 60
           MAAGLS  AV  KP +    +S  +  P +  + PP++  N       RR+ + TVC +V
Sbjct: 1   MAAGLSTIAVTLKPLN---RSSFSANHPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEMENLADEGSPMIVVPQIPSPRVAEKLARKRSERFTYLVAAVMSSFGITSMAV 120
           E++  S  M++     S       + + R+ +K  +K+SERFTYL+AAVMSSFGITSMA+
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEISFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
           MAVYYRF WQM+GGE+S  EMFGTF+LSVGA                             
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGA----------------------------- 180

Query: 181 PREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
                                                   AGLGIT+FGMAYMFVHDGLV
Sbjct: 181 ----------------------------------------AGLGITMFGMAYMFVHDGLV 225

Query: 241 HKRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGMEELEKEISRR 300
           HKRFPVGPIANVPY R+VAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG EELEKEISRR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 225

Query: 301 IK 302
           IK
Sbjct: 301 IK 225

The following BLAST results are available for this feature:

BLAST of MS000435 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141331.1	9.0e-175	99.04	beta-carotene hydroxylase 2, chloroplastic [Momordica charantia]	[more]
XP_038877448.1	4.8e-160	90.03	beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida]	[more]
KAG7014750.1	6.9e-159	90.35	Beta-carotene 3-hydroxylase 1, chloroplastic [Cucurbita argyrosperma subsp. argy...	[more]
KAG7013258.1	1.5e-158	89.71	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_004140758.1	7.7e-158	88.75	beta-carotene hydroxylase 2, chloroplastic [Cucumis sativus] >KGN57410.1 hypothe...	[more]

BLAST of MS000435 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	7.0e-122	75.69	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
Q9SZZ8	4.4e-116	70.57	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
B3SGL0	3.2e-114	69.74	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9LTG0	4.6e-113	69.54	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	2.5e-111	69.42	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]

BLAST of MS000435 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIV1	4.4e-175	99.04	beta-carotene hydroxylase 2, chloroplastic OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A0A0L606	3.7e-158	88.75	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1J1S8	6.3e-158	89.71	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1E4L5	6.3e-158	89.39	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1FZ94	1.4e-157	88.75	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata...	[more]

BLAST of MS000435 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	3.1e-117	70.57	beta-hydroxylase 1	[more]
AT5G52570.1	3.3e-114	69.54	beta-carotene hydroxylase 2	[more]
AT4G25700.2	8.4e-70	64.44	beta-hydroxylase 1	[more]
AT5G52570.2	4.8e-65	49.01	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 150..277 e-value: 2.5E-13 score: 50.5
None	No IPR available	PANTHER	PTHR31899:SF10	BETA-CAROTENE HYDROXYLASE	coord: 2..302
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 2..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000435.1	MS000435.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity