MS000424 (gene) Bitter gourd (TR) v1

Overview


Name	MS000424
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	rRNA 2'-O-methyltransferase fibrillarin
Location	scaffold44: 1613681 .. 1616303 (-)
RNA-Seq Expression	MS000424
Synteny	MS000424

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGATGATACGTGCAGGCCGCGGTGGTGGTGGTGGATTTAGGGGCAGGGGTGATGGAGGAAGGGGTGGTAGAGGTAGAGGCGGTGGTGGAGGTAGAGGTGGTGGTGGAGGCAGAGGTATGGCAAGCCGTGGTGGTGGTCGCGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGCGGTGGTATGAAAGGTGGAAGCAAAGTTGTGATTGAACCTCACAGACATGAAGGCATTTTCATAGCCAAGGGCAAGGAAGATGCTCTTGTCACTAAGAATATGGTCAGTGGTGAATCAGTCTACAATGAGAAGAGGATATCCGTACAGGTGATTCACTTGTAATCTTGCCCTTGAAATGCTTGCGCCTGAATGTTTGTAAGTTTTGAAAGTATACTTCTTGAAATCGAGGGAAACCATTCTCATATGAAAAACTTCGACCTTGTGAGTGTTTGATTATTATCCGTGAGAAATTCTAGTTGGAAATAATGTCTCCAAATAGTTTTGCGTGGTTCTGGCGCAAGAGTTATGAAGTTCATATGCCTGTAAATATTTTTAACTTTCAAGTGCATCATTAATGCATGGTCTATTGTTCCAACTTGAATGCCTTCAATTTTTGCATGTATTCAGCTCTATGTTTTGGGTGTGTTTGTAAGTTGTCTACAAAGCTAAATATTTTATATATACTGTCCTTCCAGAATGAAGATGGAACTAAAGTTGAATACAGAGTTTGGAACCCATTCAGGTCCAAGTTGGCAGCTGCAGTTCTTGGTGGTGTTGATGACATATGGATTGTTAGTGCTTTTTACCCGAAAATTTGGTTTTATCTACTTCTCTTTTCAAATTTTGTTTTATTTTTAATTTGTTAACATATGTACTGCAGAAACCTGGTGCTCGAGTGTTGTATCTTGGTGCTGCTTCAGGAACAACAGTATCGCATGTCTCAGATGTTGTTGGGCCTGTAAGTAGTTATTTATAGAGTATCTGTAGTAGTCTTTGATTGAAGTAAAAGGGTGCTTAACCATTTTAGCTATGACTCTGATTTGTAGACTGGTATAGTGTATGCCGTGGAATTCTCTCACAGAAGTGGTAGAGATTTGGTCAACATGGCGAAGAAGCGCACAAATGTTATACCCATCATTGAAGATGCCAGACACCCTGCAAAATACCGAATGTTGGTTGGCATGGTGGATGTGATATTTTCAGATGTTGCTCAGCCAGATCAGGTTCATACTTTTTGCTTTTTCTGACTGGTCTTATTATTAGTGTCTTTGAACATCATGACCTTTTTTAGACTGATGGCACAACTTATATGATAAATTGAAACTATTGATTTTTTTTCTTTTCCCGATTGGTTTTTAAGTGGTATGGTACGTTTCAAGCTGTGGTTTTTCATTTCTACGGTTTTGTTGTCGTTTGATTTAATGCTTCTAATATTTTCATCTCTCTATGCTTGAAGCATTTTTCTCTTTGAAATATTTAGCACATGACGAATTGCTCGGATTCCCCTTCAAGACTTATTGAATGATGCAAGTCCCGAGTTTCTGATGTTTTATAAAGACTTACTTTTGAATTGTTGTCTTAGTTTTTCAATTCTCTCTATTTTTCCTCGTATACTTGCAGACTTTTTGTTTTCATGCCTTTTCTCCTCAAACTATTTAGCATATGACAAATTTTTTGGATTCCCCTTCAAAACTTCTTGATTGATGCAAGTCCCGAGTTTCAGATGCTTTGAAAAGAGTTGCTTTTGAATTGTCTTCCTTGTTTCTTCAAAAATTGTATCATTTTGTGAGATATTATCCAAATGCTTATTTTCAAACTTTTTCTTGGTCGTGTTTTACTTTCTCCCGTGTTTATTCATCCTATTGCTATTCTATCTCAAGTGGATAGTATTAAATCAATCAAAACTTTTTTTTTGTTTAGGTACCAGTACCACTTCCCCTGTGTTGGAAGTTCATAAGTTGCATTTCTTACTTTTGTGAAATATCTTTGTTTTCATATTGTCTTTCTGCAGATAAATTTGATATTGGACATGATGATGTATCTACTCACATTTTTTCCTTTTAGGCCCTTCATATTTGCTAAGCATGTGATGAATTACTCGGATTCCCCTTCAAGACTTCTTGAATGATGTATAACCCGAGTTTCTGATGCATCCAAGAATATCACATTTTGATATTACTTGTCATCGGTTTACCATATTCGCATTCTTGCTCAGCTGATTCTTTGAAGTCATTAACTTCTCATTCTTTCATCTCAACTTCTAGGGTTGACTTCTTTTAACTTCCCTTCGATTCAGGCTAGAATTTTGGCACTTAATGCATCGTATTTTCTCAAAGCCGGTGGCCATTTTGTTATATCAATCAAGGTGAGTTTATGATTTGCATTTTGTTAAATTCTATAGACTATATATAATCCATGAATAGAAGATAATATGGTTTATATCAAACATTTTCAGGCCAACTGTATCGACTCAACCGTTCCAGCAGAGGCAGTGTTTGCAAGTGAGGTGAATAAGCTAAAAGCAGATCAGTTCAAACCCACCGAGCAAGTTACTCTCGAACCATTCGAGCGAGATCATGCTTGTGTTGTCGGTATCTATCGGGCACCAAAGAAACAGAAGCCCGCTGCG

mRNA sequence

TTGATGATACGTGCAGGCCGCGGTGGTGGTGGTGGATTTAGGGGCAGGGGTGATGGAGGAAGGGGTGGTAGAGGTAGAGGCGGTGGTGGAGGTAGAGGTGGTGGTGGAGGCAGAGGTATGGCAAGCCGTGGTGGTGGTCGCGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGCGGTGGTATGAAAGGTGGAAGCAAAGTTGTGATTGAACCTCACAGACATGAAGGCATTTTCATAGCCAAGGGCAAGGAAGATGCTCTTGTCACTAAGAATATGGTCAGTGGTGAATCAGTCTACAATGAGAAGAGGATATCCGTACAGAATGAAGATGGAACTAAAGTTGAATACAGAGTTTGGAACCCATTCAGGTCCAAGTTGGCAGCTGCAGTTCTTGGTGGTGTTGATGACATATGGATTAAACCTGGTGCTCGAGTGTTGTATCTTGGTGCTGCTTCAGGAACAACAGTATCGCATGTCTCAGATGTTGTTGGGCCTACTGGTATAGTGTATGCCGTGGAATTCTCTCACAGAAGTGGTAGAGATTTGGTCAACATGGCGAAGAAGCGCACAAATGTTATACCCATCATTGAAGATGCCAGACACCCTGCAAAATACCGAATGTTGGTTGGCATGGTGGATGTGATATTTTCAGATGTTGCTCAGCCAGATCAGGCTAGAATTTTGGCACTTAATGCATCGTATTTTCTCAAAGCCGGTGGCCATTTTGTTATATCAATCAAGGCCAACTGTATCGACTCAACCGTTCCAGCAGAGGCAGTGTTTGCAAGTGAGGTGAATAAGCTAAAAGCAGATCAGTTCAAACCCACCGAGCAAGTTACTCTCGAACCATTCGAGCGAGATCATGCTTGTGTTGTCGGTATCTATCGGGCACCAAAGAAACAGAAGCCCGCTGCG

Coding sequence (CDS)

TTGATGATACGTGCAGGCCGCGGTGGTGGTGGTGGATTTAGGGGCAGGGGTGATGGAGGAAGGGGTGGTAGAGGTAGAGGCGGTGGTGGAGGTAGAGGTGGTGGTGGAGGCAGAGGTATGGCAAGCCGTGGTGGTGGTCGCGGAGGTGGAAGAGGTCGCGGTGGAGGAAGAGGCGGTGGTATGAAAGGTGGAAGCAAAGTTGTGATTGAACCTCACAGACATGAAGGCATTTTCATAGCCAAGGGCAAGGAAGATGCTCTTGTCACTAAGAATATGGTCAGTGGTGAATCAGTCTACAATGAGAAGAGGATATCCGTACAGAATGAAGATGGAACTAAAGTTGAATACAGAGTTTGGAACCCATTCAGGTCCAAGTTGGCAGCTGCAGTTCTTGGTGGTGTTGATGACATATGGATTAAACCTGGTGCTCGAGTGTTGTATCTTGGTGCTGCTTCAGGAACAACAGTATCGCATGTCTCAGATGTTGTTGGGCCTACTGGTATAGTGTATGCCGTGGAATTCTCTCACAGAAGTGGTAGAGATTTGGTCAACATGGCGAAGAAGCGCACAAATGTTATACCCATCATTGAAGATGCCAGACACCCTGCAAAATACCGAATGTTGGTTGGCATGGTGGATGTGATATTTTCAGATGTTGCTCAGCCAGATCAGGCTAGAATTTTGGCACTTAATGCATCGTATTTTCTCAAAGCCGGTGGCCATTTTGTTATATCAATCAAGGCCAACTGTATCGACTCAACCGTTCCAGCAGAGGCAGTGTTTGCAAGTGAGGTGAATAAGCTAAAAGCAGATCAGTTCAAACCCACCGAGCAAGTTACTCTCGAACCATTCGAGCGAGATCATGCTTGTGTTGTCGGTATCTATCGGGCACCAAAGAAACAGAAGCCCGCTGCG

Protein sequence

LMIRAGRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA

Homology

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BLAST of MS000424 vs. NCBI nr
Match: XP_022141240.1 (mediator of RNA polymerase II transcription subunit 36a-like [Momordica charantia])

HSP 1 Score: 550.1 bits (1416), Expect = 1.2e-152
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

[Close]

BLAST of MS000424 vs. NCBI nr
Match: XP_023552409.1 (mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 546.6 bits (1407), Expect = 1.4e-151
Identity = 298/300 (99.33%), Postives = 299/300 (99.67%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

[Close]

BLAST of MS000424 vs. NCBI nr
Match: XP_022945875.1 (probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita moschata] >XP_022968445.1 probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita maxima] >KAG6574193.1 rRNA 2'-O-methyltransferase fibrillarin 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 544.3 bits (1401), Expect = 6.8e-151
Identity = 296/300 (98.67%), Postives = 299/300 (99.67%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRG GGGRGMASRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGP+GIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

[Close]

BLAST of MS000424 vs. NCBI nr
Match: XP_008439295.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 36a-like [Cucumis melo])

HSP 1 Score: 543.1 bits (1398), Expect = 1.5e-150
Identity = 295/300 (98.33%), Postives = 298/300 (99.33%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVV+EPHRHEGIFIAKGKEDALVTKNMV+GESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVTGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

[Close]

BLAST of MS000424 vs. NCBI nr
Match: KAG7013251.1 (Mediator of RNA polymerase II transcription subunit 36a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543.1 bits (1398), Expect = 1.5e-150
Identity = 295/299 (98.66%), Postives = 298/299 (99.67%), Query Frame = 0


Query: 7   RGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGSK 66
           RGGGGGFRGRGDGGRGGRGRGGGGGRG GGGRGMASRGGGRGGGRGRGGGRGGGMKGGSK
Sbjct: 5   RGGGGGFRGRGDGGRGGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGGGMKGGSK 64

Query: 67  VVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSKL 126
           VV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFRSKL
Sbjct: 65  VVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSKL 124

Query: 127 AAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNMA 186
           AAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGP+GIVYAVEFSHRSGRDLVNMA
Sbjct: 125 AAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSGRDLVNMA 184

Query: 187 KKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISI 246
           KKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISI
Sbjct: 185 KKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISI 244

Query: 247 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 306
           KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 245 KANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 303

[Close]

BLAST of MS000424 vs. ExPASy Swiss-Prot
Match: Q94AH9 (rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB2 PE=1 SV=2)

HSP 1 Score: 442.2 bits (1136), Expect = 4.8e-123
Identity = 258/311 (82.96%), Postives = 272/311 (87.46%), Query Frame = 0


Query: 8   GGGGGFR-GRGDGG-RGGRGRGG-GGGRGG---GGGRGMASRGG-GRGGGRGRGGGRG-- 67
           G GGGF  GRG GG  GGRG GG  GGRGG   GGGRG + RGG GRG G  RGG RG  
Sbjct: 7   GSGGGFSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPPRGGARGGR 66

Query: 68  -----GGMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK 127
                GGMKGGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTK
Sbjct: 67  GPAGRGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTK 126

Query: 128 VEYRVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVE 187
            EYRVWNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVE
Sbjct: 127 TEYRVWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVE 186

Query: 188 FSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS 247
           FSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS
Sbjct: 187 FSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS 246

Query: 248 YFLKAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVG 305
           YFLK+GGHFVISIKANCIDSTVPAEAVF +EV KL+ +QFKP EQVTLEPFERDHACVVG
Sbjct: 247 YFLKSGGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVG 306

[Close]

BLAST of MS000424 vs. ExPASy Swiss-Prot
Match: Q9FEF8 (rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB1 PE=1 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.1e-122
Identity = 253/304 (83.22%), Postives = 266/304 (87.50%), Query Frame = 0


Query: 3   IRAGRGGGGGFRGRGDGGRG-GRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGM 62
           +  GRGGGG   GR  GGRG G GR  GGGR G  GR    RG GRG  RGRGG   GGM
Sbjct: 5   VTGGRGGGGFRGGRDGGGRGFGGGRSFGGGRSGDRGRS-GPRGRGRGAPRGRGGPPRGGM 64

Query: 63  KGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNP 122
           KGGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTKVEYRVWNP
Sbjct: 65  KGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYRVWNP 124

Query: 123 FRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRD 182
           FRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVEFSHRSGRD
Sbjct: 125 FRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRD 184

Query: 183 LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGH 242
           LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS+FLK GGH
Sbjct: 185 LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASFFLKTGGH 244

Query: 243 FVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQ 302
           FVISIKANCIDSTV AEAVF SEV KL+ +QFKP EQVTLEPFERDHACVVG YR PKKQ
Sbjct: 245 FVISIKANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKQ 304

Query: 303 KPAA 306
           K  A
Sbjct: 305 KTPA 307

[Close]

BLAST of MS000424 vs. ExPASy Swiss-Prot
Match: Q8I1F4 (rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila erecta OX=7220 GN=Fib PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.6e-110
Identity = 229/299 (76.59%), Postives = 253/299 (84.62%), Query Frame = 0


Query: 4   RAGRGG-GGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMK 63
           R GRGG GGG  G G GG GG GRGG GGRGGGGGRG   RGGG  GG GRGGG  GG K
Sbjct: 49  RGGRGGFGGGRGGGGRGGGGGGGRGGFGGRGGGGGRGGGGRGGGGRGGGGRGGG-AGGFK 108

Query: 64  GGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPF 123
           GG  V IEPHRHEG+FIA+GKEDALVT+N V G  VY EKRISV+N +G K+EYRVWNPF
Sbjct: 109 GGKTVTIEPHRHEGVFIARGKEDALVTRNFVPGSEVYGEKRISVEN-NGEKIEYRVWNPF 168

Query: 124 RSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDL 183
           RSKLAAAVLGGV+ I + PG++VLYLGAASGTTVSHVSDVVGP G+VYAVEFSHRSGRDL
Sbjct: 169 RSKLAAAVLGGVEQIHMPPGSKVLYLGAASGTTVSHVSDVVGPEGLVYAVEFSHRSGRDL 228

Query: 184 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHF 243
           +N+AKKRTN+IPIIEDARHP KYRMLVGMVD IF+DVAQPDQ RI+ALNA +FLK GGHF
Sbjct: 229 INVAKKRTNIIPIIEDARHPHKYRMLVGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHF 288

Query: 244 VISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQ 302
           VISIKA+CIDST   EAVFASEV K++AD+ KP EQ+TLEP+ERDHA VVG+YR P KQ
Sbjct: 289 VISIKASCIDSTAQPEAVFASEVKKMQADKLKPQEQLTLEPYERDHAVVVGVYRPPPKQ 345

[Close]

BLAST of MS000424 vs. ExPASy Swiss-Prot
Match: Q9W1V3 (rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila melanogaster OX=7227 GN=Fib PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 8.8e-109
Identity = 226/299 (75.59%), Postives = 251/299 (83.95%), Query Frame = 0


Query: 4   RAGRGG-GGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMK 63
           R GRGG GGG  G G GG GG GRG  GGRGGGGGRG   RGGG  GG GRGGG  GG K
Sbjct: 48  RGGRGGFGGGRGGGGRGGGGGGGRGAFGGRGGGGGRGGGGRGGGGRGGGGRGGG-AGGFK 107

Query: 64  GGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPF 123
           GG  V IEPHRHEG+FIA+GKEDALVT+N V G  VY EKRISV+  +G K+EYRVWNPF
Sbjct: 108 GGKTVTIEPHRHEGVFIARGKEDALVTRNFVPGSEVYGEKRISVET-NGEKIEYRVWNPF 167

Query: 124 RSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDL 183
           RSKLAAAVLGGV+ I + PG++VLYLGAASGTTVSHVSDVVGP G+VYAVEFSHRSGRDL
Sbjct: 168 RSKLAAAVLGGVEQIHMPPGSKVLYLGAASGTTVSHVSDVVGPEGLVYAVEFSHRSGRDL 227

Query: 184 VNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHF 243
           +N+AKKRTN+IPIIEDARHP KYRMLVGMVD IF+DVAQPDQ RI+ALNA +FLK GGHF
Sbjct: 228 INVAKKRTNIIPIIEDARHPHKYRMLVGMVDTIFADVAQPDQGRIVALNAQHFLKNGGHF 287

Query: 244 VISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQ 302
           VISIKA+CIDST   EAVFA+EV K++AD+ KP EQ+TLEP+ERDHA VVG+YR P KQ
Sbjct: 288 VISIKASCIDSTAQPEAVFAAEVKKMQADKLKPQEQLTLEPYERDHAVVVGVYRPPPKQ 344

[Close]

BLAST of MS000424 vs. ExPASy Swiss-Prot
Match: P22509 (rRNA 2'-O-methyltransferase fibrillarin OS=Rattus norvegicus OX=10116 GN=Fbl PE=1 SV=2)

HSP 1 Score: 388.7 bits (997), Expect = 6.3e-107
Identity = 225/304 (74.01%), Postives = 253/304 (83.22%), Query Frame = 0


Query: 6   GRGGGGGF-RGRGDGGRGGRGRGGGG----GRGGGGGRGMASRGGGRGGGRGRGGGRGG- 65
           GRGG GGF  GRG  G GGRGRGGGG    GRGGGGGRG    G   GGGRGRGGGRGG 
Sbjct: 27  GRGGRGGFGGGRGGFGGGGRGRGGGGGGFRGRGGGGGRG---GGFQSGGGRGRGGGRGGK 86

Query: 66  -GMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRV 125
            G + G  V++EPHRHEG+FI +GKEDALVTKN+V GESVY EKR+S+ +E   K+EYR 
Sbjct: 87  RGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSI-SEGDDKIEYRA 146

Query: 126 WNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRS 185
           WNPFRSKLAAA+LGGVD I IKPGA+VLYLGAASGTTVSHVSD+VGP G+VYAVEFSHRS
Sbjct: 147 WNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRS 206

Query: 186 GRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKA 245
           GRDL+N+AKKRTN+IP+IEDARHP KYRML+ MVDVIF+DVAQPDQ RI+ALNA  FL+ 
Sbjct: 207 GRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRN 266

Query: 246 GGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAP 303
           GGHFVISIKANCIDST  AEAVFASEV K++ +  KP EQ+TLEP+ERDHA VVG+YR P
Sbjct: 267 GGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPP 326

[Close]

BLAST of MS000424 vs. ExPASy TrEMBL
Match: A0A6J1CHH3 (mediator of RNA polymerase II transcription subunit 36a-like OS=Momordica charantia OX=3673 GN=LOC111011688 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.0e-153
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

[Close]

BLAST of MS000424 vs. ExPASy TrEMBL
Match: A0A6J1HX74 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita maxima OX=3661 GN=LOC111467690 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 296/300 (98.67%), Postives = 299/300 (99.67%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRG GGGRGMASRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGP+GIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

[Close]

BLAST of MS000424 vs. ExPASy TrEMBL
Match: A0A6J1G280 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita moschata OX=3662 GN=LOC111449984 PE=3 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 3.3e-151
Identity = 296/300 (98.67%), Postives = 299/300 (99.67%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRG GGGRGMASRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGFGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGP+GIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPSGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305

[Close]

BLAST of MS000424 vs. ExPASy TrEMBL
Match: A0A1S3AY25 (mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo OX=3656 GN=LOC103484122 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 7.4e-151
Identity = 295/300 (98.33%), Postives = 298/300 (99.33%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMQSRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVV+EPHRHEGIFIAKGKEDALVTKNMV+GESVYNEKRISVQNEDGTK+EYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVTGESVYNEKRISVQNEDGTKIEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 305

[Close]

BLAST of MS000424 vs. ExPASy TrEMBL
Match: A0A6J1JA27 (probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita maxima OX=3661 GN=LOC111483086 PE=3 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 4.8e-150
Identity = 297/300 (99.00%), Postives = 298/300 (99.33%), Query Frame = 0


Query: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGMKGGS 65
           GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGM SRGGGRGGGRGRGGGRGGGMKGGS
Sbjct: 6   GRGGGGGFRGRGDGGRGGRGRGGGGGRGGGGGRGM-SRGGGRGGGRGRGGGRGGGMKGGS 65

Query: 66  KVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125
           KVV+EPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK
Sbjct: 66  KVVVEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSK 125

Query: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185
           LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM
Sbjct: 126 LAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNM 185

Query: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245
           AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS
Sbjct: 186 AKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVIS 245

Query: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKPAA 305
           IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQK AA
Sbjct: 246 IKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQKAAA 304

[Close]

BLAST of MS000424 vs. TAIR 10
Match: AT4G25630.1 (fibrillarin 2 )

HSP 1 Score: 442.2 bits (1136), Expect = 3.4e-124
Identity = 258/311 (82.96%), Postives = 272/311 (87.46%), Query Frame = 0


Query: 8   GGGGGFR-GRGDGG-RGGRGRGG-GGGRGG---GGGRGMASRGG-GRGGGRGRGGGRG-- 67
           G GGGF  GRG GG  GGRG GG  GGRGG   GGGRG + RGG GRG G  RGG RG  
Sbjct: 7   GSGGGFSGGRGRGGYSGGRGDGGFSGGRGGGGRGGGRGFSDRGGRGRGRGPPRGGARGGR 66

Query: 68  -----GGMKGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTK 127
                GGMKGGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTK
Sbjct: 67  GPAGRGGMKGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTK 126

Query: 128 VEYRVWNPFRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVE 187
            EYRVWNPFRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVE
Sbjct: 127 TEYRVWNPFRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVE 186

Query: 188 FSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS 247
           FSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS
Sbjct: 187 FSHRSGRDLVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS 246

Query: 248 YFLKAGGHFVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVG 305
           YFLK+GGHFVISIKANCIDSTVPAEAVF +EV KL+ +QFKP EQVTLEPFERDHACVVG
Sbjct: 247 YFLKSGGHFVISIKANCIDSTVPAEAVFQTEVKKLQQEQFKPAEQVTLEPFERDHACVVG 306

[Close]

BLAST of MS000424 vs. TAIR 10
Match: AT5G52470.1 (fibrillarin 1 )

HSP 1 Score: 441.0 bits (1133), Expect = 7.6e-124
Identity = 253/304 (83.22%), Postives = 266/304 (87.50%), Query Frame = 0


Query: 3   IRAGRGGGGGFRGRGDGGRG-GRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGM 62
           +  GRGGGG   GR  GGRG G GR  GGGR G  GR    RG GRG  RGRGG   GGM
Sbjct: 5   VTGGRGGGGFRGGRDGGGRGFGGGRSFGGGRSGDRGRS-GPRGRGRGAPRGRGGPPRGGM 64

Query: 63  KGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNP 122
           KGGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTKVEYRVWNP
Sbjct: 65  KGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYRVWNP 124

Query: 123 FRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRD 182
           FRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVEFSHRSGRD
Sbjct: 125 FRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRD 184

Query: 183 LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGH 242
           LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNAS+FLK GGH
Sbjct: 185 LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASFFLKTGGH 244

Query: 243 FVISIKANCIDSTVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKKQ 302
           FVISIKANCIDSTV AEAVF SEV KL+ +QFKP EQVTLEPFERDHACVVG YR PKKQ
Sbjct: 245 FVISIKANCIDSTVAAEAVFQSEVKKLQQEQFKPAEQVTLEPFERDHACVVGGYRMPKKQ 304

Query: 303 KPAA 306
           K  A
Sbjct: 305 KTPA 307

[Close]

BLAST of MS000424 vs. TAIR 10
Match: AT5G52490.1 (Fibrillarin family protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.6e-94
Identity = 197/287 (68.64%), Postives = 227/287 (79.09%), Query Frame = 0


Query: 24  RGRGGG--GGRG-GGGGRGMASRGGGRGG--GRGRGGGR-----GGGMKGGSKVVIEPHR 83
           RGRGGG  GGRG   GG G A RG GRGG  GRGRG GR      GG+KGGSKV++ PHR
Sbjct: 6   RGRGGGVRGGRGLARGGEGSAVRGSGRGGESGRGRGPGRVKSESDGGIKGGSKVLVTPHR 65

Query: 84  HEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNPFRSKLAAAVLGG 143
           H G+F+AK K DALVTKN+V GE +YNEKRI VQNED + VEYRVWNP RSKLA A+  G
Sbjct: 66  HAGVFVAKSKADALVTKNLVPGEIIYNEKRIFVQNEDRSTVEYRVWNPHRSKLADAITTG 125

Query: 144 VDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRDLVNMAKKRTNVI 203
           VD+IWIKPG +VLYLGA+SG TVSHVSD+VGP G VYAVE S   G+ L+NMA+KRTNVI
Sbjct: 126 VDNIWIKPGVKVLYLGASSGYTVSHVSDIVGPEGCVYAVEHSDICGKVLMNMAEKRTNVI 185

Query: 204 PIIEDARHPAKYRMLVGMVDVIFSDVAQPDQARILALNASYFLKAGGHFVISIKANCIDS 263
           PIIEDARHPAKYRMLVGMVD+IFSDV  P+QA IL+LNASYFLK+GGHF+ISIKAN IDS
Sbjct: 186 PIIEDARHPAKYRMLVGMVDIIFSDVNHPEQANILSLNASYFLKSGGHFMISIKANSIDS 245

Query: 264 TVPAEAVFASEVNKLKADQFKPTEQVTLEPFERDHACVVGIYRAPKK 301
           T+ AE V+  EV KL+ ++ +PTE + L+  E  HACV G YR P+K
Sbjct: 246 TIAAETVYQMEVEKLQMEELRPTEILHLDSCEEKHACVFGGYRLPRK 292

[Close]

BLAST of MS000424 vs. TAIR 10
Match: AT5G52470.2 (fibrillarin 1 )

HSP 1 Score: 309.7 bits (792), Expect = 2.6e-84
Identity = 185/223 (82.96%), Postives = 195/223 (87.44%), Query Frame = 0


Query: 3   IRAGRGGGGGFRGRGDGGRG-GRGRGGGGGRGGGGGRGMASRGGGRGGGRGRGGGRGGGM 62
           +  GRGGGG   GR  GGRG G GR  GGGR G  GR    RG GRG  RGRGG   GGM
Sbjct: 5   VTGGRGGGGFRGGRDGGGRGFGGGRSFGGGRSGDRGRS-GPRGRGRGAPRGRGGPPRGGM 64

Query: 63  KGGSKVVIEPHRHEGIFIAKGKEDALVTKNMVSGESVYNEKRISVQNEDGTKVEYRVWNP 122
           KGGSKV++EPHRH G+FIAKGKEDALVTKN+V GE+VYNEKRISVQNEDGTKVEYRVWNP
Sbjct: 65  KGGSKVIVEPHRHAGVFIAKGKEDALVTKNLVPGEAVYNEKRISVQNEDGTKVEYRVWNP 124

Query: 123 FRSKLAAAVLGGVDDIWIKPGARVLYLGAASGTTVSHVSDVVGPTGIVYAVEFSHRSGRD 182
           FRSKLAAA+LGGVD+IWIKPGA+VLYLGAASGTTVSHVSD+VGP G VYAVEFSHRSGRD
Sbjct: 125 FRSKLAAAILGGVDNIWIKPGAKVLYLGAASGTTVSHVSDLVGPEGCVYAVEFSHRSGRD 184

Query: 183 LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQ 225
           LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQ
Sbjct: 185 LVNMAKKRTNVIPIIEDARHPAKYRMLVGMVDVIFSDVAQPDQ 226

The following BLAST results are available for this feature:

BLAST of MS000424 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141240.1	1.2e-152	100.00	mediator of RNA polymerase II transcription subunit 36a-like [Momordica charanti...	[more]
XP_023552409.1	1.4e-151	99.33	mediator of RNA polymerase II transcription subunit 36a-like [Cucurbita pepo sub...	[more]
XP_022945875.1	6.8e-151	98.67	probable mediator of RNA polymerase II transcription subunit 36b [Cucurbita mosc...	[more]
XP_008439295.1	1.5e-150	98.33	PREDICTED: mediator of RNA polymerase II transcription subunit 36a-like [Cucumis...	[more]
KAG7013251.1	1.5e-150	98.66	Mediator of RNA polymerase II transcription subunit 36a, partial [Cucurbita argy...	[more]

BLAST of MS000424 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AH9	4.8e-123	82.96	rRNA 2'-O-methyltransferase fibrillarin 2 OS=Arabidopsis thaliana OX=3702 GN=FIB...	[more]
Q9FEF8	1.1e-122	83.22	rRNA 2'-O-methyltransferase fibrillarin 1 OS=Arabidopsis thaliana OX=3702 GN=FIB...	[more]
Q8I1F4	1.6e-110	76.59	rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila erecta OX=7220 GN=Fib PE=3...	[more]
Q9W1V3	8.8e-109	75.59	rRNA 2'-O-methyltransferase fibrillarin OS=Drosophila melanogaster OX=7227 GN=Fi...	[more]
P22509	6.3e-107	74.01	rRNA 2'-O-methyltransferase fibrillarin OS=Rattus norvegicus OX=10116 GN=Fbl PE=...	[more]

BLAST of MS000424 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHH3	6.0e-153	100.00	mediator of RNA polymerase II transcription subunit 36a-like OS=Momordica charan...	[more]
A0A6J1HX74	3.3e-151	98.67	probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita ma...	[more]
A0A6J1G280	3.3e-151	98.67	probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita mo...	[more]
A0A1S3AY25	7.4e-151	98.33	mediator of RNA polymerase II transcription subunit 36a-like OS=Cucumis melo OX=...	[more]
A0A6J1JA27	4.8e-150	99.00	probable mediator of RNA polymerase II transcription subunit 36b OS=Cucurbita ma...	[more]

BLAST of MS000424 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25630.1	3.4e-124	82.96	fibrillarin 2	[more]
AT5G52470.1	7.6e-124	83.22	fibrillarin 1	[more]
AT5G52490.1	1.6e-94	68.64	Fibrillarin family protein	[more]
AT5G52470.2	2.6e-84	82.96	fibrillarin 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000692	Fibrillarin	PRINTS	PR00052	FIBRILLARIN	coord: 87..106 score: 64.29 coord: 192..221 score: 70.24 coord: 222..251 score: 67.62 coord: 274..295 score: 61.36 coord: 115..134 score: 75.71 coord: 142..163 score: 79.22 coord: 167..184 score: 72.22
IPR000692	Fibrillarin	SMART	SM01206	Fibrillarin_2	coord: 69..297 e-value: 8.6E-173 score: 590.0
IPR000692	Fibrillarin	PFAM	PF01269	Fibrillarin	coord: 69..296 e-value: 1.1E-109 score: 364.9
IPR000692	Fibrillarin	PIRSF	PIRSF006540	Nop17p	coord: 19..299 e-value: 5.6E-121 score: 400.5
IPR000692	Fibrillarin	HAMAP	MF_00351	RNA_methyltransf_FlpA	coord: 83..296 score: 36.980152
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 64..119 e-value: 1.6E-29 score: 103.5
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 120..297 e-value: 1.6E-88 score: 296.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..66
None	No IPR available	PANTHER	PTHR10335:SF22	FIBRILLARIN, S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE-RELATED	coord: 4..304
None	No IPR available	PANTHER	PTHR10335	RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN	coord: 4..304
IPR020813	Fibrillarin, conserved site	PROSITE	PS00566	FIBRILLARIN	coord: 167..181
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 67..295

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000424.1	MS000424.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006364	rRNA processing
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding