MS000411 (gene) Bitter gourd (TR) v1

BLAST of MS000411 vs. NCBI nr
Match: XP_022141038.1 (pectin acetylesterase 9 [Momordica charantia])

HSP 1 Score: 825.1 bits (2130), Expect = 2.6e-235
Identity = 396/400 (99.00%), Postives = 396/400 (99.00%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           MKPAAVEAS VALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG
Sbjct: 1   MKPAAVEASFVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAG GNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGTGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDL ALQGMEPN
Sbjct: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLAALQGMEPN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK
Sbjct: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP
Sbjct: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP
Sbjct: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 399

BLAST of MS000411 vs. NCBI nr
Match: XP_038876631.1 (pectin acetylesterase 9 isoform X1 [Benincasa hispida])

HSP 1 Score: 746.1 bits (1925), Expect = 1.5e-211
Identity = 360/400 (90.00%), Postives = 379/400 (94.75%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M P AVEA L+ L+LLA+ALR P  A ARLLVEMT+VRNASSIGA CLDGSLPAYHL RG
Sbjct: 1   MSPPAVEAPLLFLILLAAALRCPL-AGARLLVEMTVVRNASSIGAFCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDVKSCLERA++RRGSTRLMTKWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERANSRRGSTRLMTKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLP GL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPNGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLAAFLHCENF+KYLP NA+VKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGME N
Sbjct: 181 AGGLAAFLHCENFSKYLPRNASVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEQN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNENC ASSY+P+LCIFPQ+SLKYITTPFFILNSAYDV+QFHHGLVPPS+DLHGHWNHCK
Sbjct: 241 LNENCKASSYFPELCIFPQHSLKYITTPFFILNSAYDVFQFHHGLVPPSADLHGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
            +PA CSE QI VLQGLR+SMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFA++SP
Sbjct: 301 FNPAECSELQIQVLQGLRISMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFADDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TIDNKTIAEAVGDWYFS RRNIKEIDCAYPCDS+CHNIIP
Sbjct: 361 TIDNKTIAEAVGDWYFS-RRNIKEIDCAYPCDSTCHNIIP 397

BLAST of MS000411 vs. NCBI nr
Match: XP_011651157.1 (pectin acetylesterase 9 isoform X1 [Cucumis sativus])

HSP 1 Score: 738.4 bits (1905), Expect = 3.2e-209
Identity = 356/400 (89.00%), Postives = 374/400 (93.50%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M P AVEA L+ L LLA+AL  P G  ARLLVEMT+VRNASSIGALCLDGSLPAYHL RG
Sbjct: 1   MSPTAVEAPLLLLFLLAAALLCPLGG-ARLLVEMTVVRNASSIGALCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDV SC ERA++RRGST LMTKWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVTSCFERANSRRGSTHLMTKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASFAGDALFDNGTS+L+FKGQKIWESIILDLLPKGL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFAGDALFDNGTSVLHFKGQKIWESIILDLLPKGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLA FLHCENFT YLP NA+VKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGME N
Sbjct: 181 AGGLATFLHCENFTNYLPRNASVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMERN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNENCTASSY+P+LCIFPQYSLKYITTPFFILNSAYDV+QFHHGLVPPS+DLHGHWNHCK
Sbjct: 241 LNENCTASSYFPELCIFPQYSLKYITTPFFILNSAYDVFQFHHGLVPPSADLHGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           L+PA CSE QI VLQGLR+SMLSALRLF+EYSSRGGMFINSCFAHCQSESQDTWFA +SP
Sbjct: 301 LNPADCSELQIQVLQGLRLSMLSALRLFHEYSSRGGMFINSCFAHCQSESQDTWFAPDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TIDNKTIAEAVGDWYF  RRNIKEIDCAYPCDS+CHNIIP
Sbjct: 361 TIDNKTIAEAVGDWYFG-RRNIKEIDCAYPCDSTCHNIIP 397

BLAST of MS000411 vs. NCBI nr
Match: XP_022985445.1 (pectin acetylesterase 9 [Cucurbita maxima])

HSP 1 Score: 736.5 bits (1900), Expect = 1.2e-208
Identity = 352/400 (88.00%), Postives = 378/400 (94.50%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M  AAVEA L+ LLLLA+ALRWP  A ARLLVEMT+V+NASSIGA CLDGSLPAYHL RG
Sbjct: 1   MNLAAVEAPLLVLLLLAAALRWPL-AGARLLVEMTVVQNASSIGAFCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDVKSCLERA++RRGSTRLM KWEDFSGILSN+ASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERANSRRGSTRLMNKWEDFSGILSNDASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASF G+ALFDNGTSLLYFKGQKIWESIILDLLP+GL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFGGNALFDNGTSLLYFKGQKIWESIILDLLPRGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLA FLHCENF+KYLP NA+VKCLSDAGFFLDLRDIGLNHTMRNFY+DLVALQGM+ N
Sbjct: 181 AGGLATFLHCENFSKYLPRNASVKCLSDAGFFLDLRDIGLNHTMRNFYRDLVALQGMQQN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNE+CTA+SY+P+LCIFPQYSLKYITTPFF+LNSAYDV+QFHHGLVPPS+DL GHWNHCK
Sbjct: 241 LNEDCTAASYFPELCIFPQYSLKYITTPFFVLNSAYDVFQFHHGLVPPSADLRGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           L+PA CSEQQI VLQGLR+SMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWF E+SP
Sbjct: 301 LNPAECSEQQIQVLQGLRISMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFGEDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TID+KTIAEAVGDWYFS RRN KEIDCAYPCDS+CHNIIP
Sbjct: 361 TIDDKTIAEAVGDWYFS-RRNTKEIDCAYPCDSTCHNIIP 397

BLAST of MS000411 vs. NCBI nr
Match: XP_022922469.1 (pectin acetylesterase 9 [Cucurbita moschata] >KAG6576722.1 Pectin acetylesterase 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 736.1 bits (1899), Expect = 1.6e-208
Identity = 352/400 (88.00%), Postives = 376/400 (94.00%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M  AAVEA L+ LLLLA+ALRWP  A ARLLVEMT+V+NASSIGA CLDGSLPAYHL RG
Sbjct: 1   MNLAAVEAPLLVLLLLAAALRWPL-AGARLLVEMTVVQNASSIGAFCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDVKSCLERA++RRGSTRLM KWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERANSRRGSTRLMNKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASF G+ALFDNGTSLLYFKGQKIWESIILDLLPKGL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFGGNALFDNGTSLLYFKGQKIWESIILDLLPKGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLA FLHCENF+KYLP NA+VKCLSDAGFFLDLRD GLNHTMRNFY+DLVALQGM+ N
Sbjct: 181 AGGLATFLHCENFSKYLPRNASVKCLSDAGFFLDLRDTGLNHTMRNFYRDLVALQGMQQN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNE+CTA+SY+P+LCIFPQYSLKYITTPFF+LNSAYDV+QFHHGLVPPS+DL GHWNHCK
Sbjct: 241 LNEDCTAASYFPELCIFPQYSLKYITTPFFVLNSAYDVFQFHHGLVPPSADLRGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           L+PA CSEQQI VLQGLR+SMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWF E+SP
Sbjct: 301 LNPAECSEQQIQVLQGLRISMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFGEDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TID+KTIAEAVGDWYF  RRN KEIDCAYPCDS+CHNIIP
Sbjct: 361 TIDDKTIAEAVGDWYFG-RRNTKEIDCAYPCDSTCHNIIP 397

BLAST of MS000411 vs. ExPASy Swiss-Prot
Match: B9DFR3 (Pectin acetylesterase 9 OS=Arabidopsis thaliana OX=3702 GN=PAE9 PE=2 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.0e-157
Identity = 254/370 (68.65%), Postives = 300/370 (81.08%), Query Frame = 0

Query: 32  VEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERASTR 91
           V MTLVR+A+++GA CLDGSLPAYHL RGFGAG+ NW+LQFEGGGWCND+ SC+ERA TR
Sbjct: 35  VSMTLVRDAAALGAFCLDGSLPAYHLDRGFGAGSNNWILQFEGGGWCNDIASCVERAKTR 94

Query: 92  RGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLYFK 151
           RGSTR M+K   F+G+LSNNAS NP DFYNWN+V+LRYCDGASFAGD+ F NGTSLLYF+
Sbjct: 95  RGSTRYMSKTVVFTGVLSNNASQNP-DFYNWNKVRLRYCDGASFAGDSQFGNGTSLLYFR 154

Query: 152 GQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDAGF 211
           GQ+IW +IILDLLPKGLA A KALL+GCSAGGL+ FLHC+NFT YLP NA+VKC+SDAGF
Sbjct: 155 GQRIWNAIILDLLPKGLAKAHKALLTGCSAGGLSTFLHCDNFTSYLPKNASVKCMSDAGF 214

Query: 212 FLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSY-YPDLCIFPQYSLKYITTPFF 271
           FLD  D+  N TMR+FY  LV+LQG++ NL+ +CT + +  P LC FPQY L++I TPFF
Sbjct: 215 FLDAIDVAANRTMRSFYSQLVSLQGIQKNLDPSCTHAFFPEPSLCFFPQYVLRFIKTPFF 274

Query: 272 ILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFYE 331
           ILNSAYDV+QFHHGLVPPS+D  G WN CKL+  AC+  Q+D LQG R  ML AL  F+ 
Sbjct: 275 ILNSAYDVFQFHHGLVPPSADQTGRWNRCKLNVTACNPHQLDALQGFRKDMLGALMNFFR 334

Query: 332 YSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAYP 391
            S+RGGMFINSCF HCQS  ++TW +  SP I+NKTIAE VGDWYF R    KEI C YP
Sbjct: 335 NSTRGGMFINSCFDHCQSALEETWLSPTSPRINNKTIAETVGDWYFGRGEEAKEIGCPYP 394

Query: 392 CDSSCHNIIP 401
           CD +CHN+IP
Sbjct: 395 CDKTCHNLIP 403

BLAST of MS000411 vs. ExPASy Swiss-Prot
Match: O80731 (Pectin acetylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=PAE3 PE=2 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 9.7e-116
Identity = 197/370 (53.24%), Postives = 258/370 (69.73%), Query Frame = 0

Query: 30  LLVEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERAS 89
           L+V +TL++ A+S GA+CLDG+LP YHLH G G+GA  WL+Q EGGGWCN  +SC+ R +
Sbjct: 51  LMVPLTLIQAAASKGAVCLDGTLPGYHLHPGSGSGANRWLIQLEGGGWCNTRRSCIFRKT 110

Query: 90  TRRGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLY 149
           TRRGS+  M K   F+GILSN ++ NP DF+NWNRVKLRYCDGASF GD+   + +S LY
Sbjct: 111 TRRGSSNHMEKVLAFTGILSNKSNENP-DFFNWNRVKLRYCDGASFTGDS--QDESSQLY 170

Query: 150 FKGQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDA 209
           ++GQ+IW S + +LL KG+  A++ALLSGCSAGGLA+ LHC+ F +  P   TVKCLSDA
Sbjct: 171 YRGQRIWHSAMEELLSKGMQKAEQALLSGCSAGGLASILHCDQFKELFPGTTTVKCLSDA 230

Query: 210 GFFLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSYYPDLCIFPQYSLKYITTPF 269
           G F+D  D+   H++R  ++ +V +Q ++  L+  CT     P  C FPQ  +  I TP 
Sbjct: 231 GMFMDAVDVSGGHSLRKMFQGVVTVQNLQKELSTACT-KHLDPTSCFFPQNLVSGIKTPM 290

Query: 270 FILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFY 329
           F+LN+AYD +Q    L PPS DL G W  CK D + C+  QI   Q  R  M+ A++ F 
Sbjct: 291 FLLNAAYDAWQVQESLAPPSVDLSGSWKACKSDHSHCNSSQIQFFQDFRTHMVDAVKSF- 350

Query: 330 EYSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAY 389
             S+  G+FINSCFAHCQSE QDTW+A +SPT+  KT+AE+VGDWYF  R  +K IDC Y
Sbjct: 351 ATSTHNGVFINSCFAHCQSERQDTWYAPDSPTLHGKTVAESVGDWYFD-RTTVKAIDCPY 410

Query: 390 PCDSSCHNII 400
           PCD +CHN+I
Sbjct: 411 PCDKTCHNLI 414

BLAST of MS000411 vs. ExPASy Swiss-Prot
Match: F4I839 (Pectin acetylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PAE2 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-115
Identity = 197/369 (53.39%), Postives = 261/369 (70.73%), Query Frame = 0

Query: 31  LVEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERAST 90
           +V +TL+++A++ GA+CLDGSLP YHLHRGFG+GA NWL+Q EGGGWC+ +++C+ R +T
Sbjct: 79  MVGLTLIQSAAAKGAVCLDGSLPGYHLHRGFGSGANNWLVQLEGGGWCDTIRNCVYRKTT 138

Query: 91  RRGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLYF 150
           RRGS+  M K   F+GILS+ A+ NP DFYNWNRVK+RYCDG SF+GD+  +N  + L F
Sbjct: 139 RRGSSSYMEKEIPFTGILSDKAADNP-DFYNWNRVKVRYCDGGSFSGDS--ENKAAQLQF 198

Query: 151 KGQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDAG 210
           +G++IW + + DL+ KG+  A++ALLSGCSAGGLA  L C++F K  P +  VKCLSDAG
Sbjct: 199 RGKRIWLAAMEDLMAKGMRQAKQALLSGCSAGGLAVILRCDDFGKLFPPSTRVKCLSDAG 258

Query: 211 FFLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSYYPDLCIFPQYSLKYITTPFF 270
           FFLD  D+    ++R  Y  +V LQ ++ NL + C  +   P  C FPQ  +  + TP F
Sbjct: 259 FFLDAIDVSGGRSLRRLYAGVVRLQNLQTNLPQYC-VNRLNPTSCFFPQNLINQVKTPLF 318

Query: 271 ILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFYE 330
           ILN+AYD +Q    L P S+D  G WN C+L+ A CS  QI  LQG R  M++ ++ F  
Sbjct: 319 ILNAAYDSWQIQESLAPKSADPSGSWNDCRLNYAKCSASQIQFLQGFRTRMVNLVKGF-A 378

Query: 331 YSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAYP 390
             S+ G+F+NSCFAHCQ+E  DTWFA+NSP I NK IA AVGDWYF  R   K IDCAYP
Sbjct: 379 MPSKNGVFLNSCFAHCQTERHDTWFAQNSPAIKNKGIAVAVGDWYF-ERGGAKLIDCAYP 438

Query: 391 CDSSCHNII 400
           CD +CHN++
Sbjct: 439 CDKTCHNLV 441

BLAST of MS000411 vs. ExPASy Swiss-Prot
Match: Q84JS1 (Pectin acetylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=PAE6 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-115
Identity = 189/369 (51.22%), Postives = 263/369 (71.27%), Query Frame = 0

Query: 31  LVEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERAST 90
           ++ +TL+  A S GA+CLDG+LP YHL RGFG+GA +WL+Q EGGGWCN+ +SC+ R ++
Sbjct: 54  MIPLTLIHGADSKGAVCLDGTLPGYHLDRGFGSGANSWLIQLEGGGWCNNHRSCVYRKTS 113

Query: 91  RRGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLYF 150
           RRGS++ M K   F+GILSN +  NP DF+NWNR+KLRYCDGASF+GD+   + +S L++
Sbjct: 114 RRGSSKFMEKALAFTGILSNRSEENP-DFFNWNRIKLRYCDGASFSGDS--QDESSQLFY 173

Query: 151 KGQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDAG 210
           +GQ+IW+  + + L  G+  A +ALLSGCSAGGLA+ LHC+ F + LP++  VKCLSDAG
Sbjct: 174 RGQRIWQVAMEEFLSLGMKQANQALLSGCSAGGLASILHCDEFRELLPSSTKVKCLSDAG 233

Query: 211 FFLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSYYPDLCIFPQYSLKYITTPFF 270
            FLD  D+   H++RN ++ +V +Q ++ +L+  CT +   P  C FPQ  +  I TP F
Sbjct: 234 MFLDSVDVSGGHSLRNMFQGVVTVQNLQKDLSSTCT-NHLDPTSCFFPQNLVSDIKTPMF 293

Query: 271 ILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFYE 330
           +LN+AYD +Q    L PP++D  G W  CK D + C+  QI   Q  R  ML A+  F  
Sbjct: 294 LLNTAYDSWQIQESLAPPTADPGGIWKACKSDHSRCNSSQIQFFQEFRNQMLFAVNSF-S 353

Query: 331 YSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAYP 390
            S + G++INSCFAHCQ+E QDTWFA++SP ++ K +AE+VGDWYF R +N+K IDC YP
Sbjct: 354 NSDQNGLYINSCFAHCQTERQDTWFAQDSPQLNGKRVAESVGDWYFDRAKNVKAIDCPYP 413

Query: 391 CDSSCHNII 400
           CD++CHN+I
Sbjct: 414 CDTTCHNLI 417

BLAST of MS000411 vs. ExPASy Swiss-Prot
Match: Q66GM8 (Pectin acetylesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PAE10 PE=2 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 3.7e-115
Identity = 197/370 (53.24%), Postives = 255/370 (68.92%), Query Frame = 0

Query: 30  LLVEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERAS 89
           L+V +TL+R A S GA+CLDG+LP YHLHRG G+GA +WL+Q EGGGWC+++++C+ R  
Sbjct: 51  LMVGLTLIRGAGSKGAVCLDGTLPGYHLHRGHGSGANSWLIQLEGGGWCDNIRNCVYRKK 110

Query: 90  TRRGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLY 149
           +RRGS+  M K   F+GILSN A  NP DF+NWNRVKLRYCDG SF+GD+   N  + L 
Sbjct: 111 SRRGSSNYMEKQIQFTGILSNKAQENP-DFFNWNRVKLRYCDGGSFSGDS--QNKAARLQ 170

Query: 150 FKGQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDA 209
           F+G+KIW + + DL  KG+  A++ALLSGCSAGGLA  L C+ F         VKCLSDA
Sbjct: 171 FRGEKIWRAAMDDLKAKGMRNAKQALLSGCSAGGLAVILRCDEFRNLFSGWTRVKCLSDA 230

Query: 210 GFFLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSYYPDLCIFPQYSLKYITTPF 269
           G FLD  D+   HT+RN Y  +V LQG++ NL   CT +   P  C FPQ  +  + TP 
Sbjct: 231 GLFLDTPDVSGGHTIRNLYNGVVQLQGVKNNLPHLCT-NHLNPTSCFFPQNLISQMKTPL 290

Query: 270 FILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFY 329
           FI+N+AYD++Q    + PPS+D  G+W+ C+L+   C+  QI  LQG R  ML A+  F 
Sbjct: 291 FIVNAAYDIWQIQSSIAPPSADPSGYWHECRLNHGRCTPAQIRFLQGFRNQMLRAVSGF- 350

Query: 330 EYSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAY 389
             S + G+FINSCFAHCQ+E QDTWFA++SP I  K +A AVGDWYF  R  +K IDC Y
Sbjct: 351 SNSKKNGLFINSCFAHCQTERQDTWFADDSPVIHKKAVAIAVGDWYFD-RAEVKLIDCPY 410

Query: 390 PCDSSCHNII 400
           PCD SCHN++
Sbjct: 411 PCDRSCHNLV 414

BLAST of MS000411 vs. ExPASy TrEMBL
Match: A0A6J1CJD6 (Pectin acetylesterase OS=Momordica charantia OX=3673 GN=LOC111011539 PE=3 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 1.3e-235
Identity = 396/400 (99.00%), Postives = 396/400 (99.00%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           MKPAAVEAS VALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG
Sbjct: 1   MKPAAVEASFVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAG GNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGTGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDL ALQGMEPN
Sbjct: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLAALQGMEPN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK
Sbjct: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP
Sbjct: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP
Sbjct: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 399

BLAST of MS000411 vs. ExPASy TrEMBL
Match: A0A0A0L8Q6 (Pectin acetylesterase OS=Cucumis sativus OX=3659 GN=Csa_3G182200 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 1.6e-209
Identity = 356/400 (89.00%), Postives = 374/400 (93.50%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M P AVEA L+ L LLA+AL  P G  ARLLVEMT+VRNASSIGALCLDGSLPAYHL RG
Sbjct: 1   MSPTAVEAPLLLLFLLAAALLCPLGG-ARLLVEMTVVRNASSIGALCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDV SC ERA++RRGST LMTKWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVTSCFERANSRRGSTHLMTKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASFAGDALFDNGTS+L+FKGQKIWESIILDLLPKGL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFAGDALFDNGTSVLHFKGQKIWESIILDLLPKGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLA FLHCENFT YLP NA+VKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGME N
Sbjct: 181 AGGLATFLHCENFTNYLPRNASVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMERN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNENCTASSY+P+LCIFPQYSLKYITTPFFILNSAYDV+QFHHGLVPPS+DLHGHWNHCK
Sbjct: 241 LNENCTASSYFPELCIFPQYSLKYITTPFFILNSAYDVFQFHHGLVPPSADLHGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           L+PA CSE QI VLQGLR+SMLSALRLF+EYSSRGGMFINSCFAHCQSESQDTWFA +SP
Sbjct: 301 LNPADCSELQIQVLQGLRLSMLSALRLFHEYSSRGGMFINSCFAHCQSESQDTWFAPDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TIDNKTIAEAVGDWYF  RRNIKEIDCAYPCDS+CHNIIP
Sbjct: 361 TIDNKTIAEAVGDWYFG-RRNIKEIDCAYPCDSTCHNIIP 397

BLAST of MS000411 vs. ExPASy TrEMBL
Match: A0A6J1JBC0 (Pectin acetylesterase OS=Cucurbita maxima OX=3661 GN=LOC111483453 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 5.9e-209
Identity = 352/400 (88.00%), Postives = 378/400 (94.50%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M  AAVEA L+ LLLLA+ALRWP  A ARLLVEMT+V+NASSIGA CLDGSLPAYHL RG
Sbjct: 1   MNLAAVEAPLLVLLLLAAALRWPL-AGARLLVEMTVVQNASSIGAFCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDVKSCLERA++RRGSTRLM KWEDFSGILSN+ASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERANSRRGSTRLMNKWEDFSGILSNDASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASF G+ALFDNGTSLLYFKGQKIWESIILDLLP+GL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFGGNALFDNGTSLLYFKGQKIWESIILDLLPRGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLA FLHCENF+KYLP NA+VKCLSDAGFFLDLRDIGLNHTMRNFY+DLVALQGM+ N
Sbjct: 181 AGGLATFLHCENFSKYLPRNASVKCLSDAGFFLDLRDIGLNHTMRNFYRDLVALQGMQQN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNE+CTA+SY+P+LCIFPQYSLKYITTPFF+LNSAYDV+QFHHGLVPPS+DL GHWNHCK
Sbjct: 241 LNEDCTAASYFPELCIFPQYSLKYITTPFFVLNSAYDVFQFHHGLVPPSADLRGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           L+PA CSEQQI VLQGLR+SMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWF E+SP
Sbjct: 301 LNPAECSEQQIQVLQGLRISMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFGEDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TID+KTIAEAVGDWYFS RRN KEIDCAYPCDS+CHNIIP
Sbjct: 361 TIDDKTIAEAVGDWYFS-RRNTKEIDCAYPCDSTCHNIIP 397

BLAST of MS000411 vs. ExPASy TrEMBL
Match: A0A6J1E3G7 (Pectin acetylesterase OS=Cucurbita moschata OX=3662 GN=LOC111430471 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 7.8e-209
Identity = 352/400 (88.00%), Postives = 376/400 (94.00%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M  AAVEA L+ LLLLA+ALRWP  A ARLLVEMT+V+NASSIGA CLDGSLPAYHL RG
Sbjct: 1   MNLAAVEAPLLVLLLLAAALRWPL-AGARLLVEMTVVQNASSIGAFCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDVKSCLERA++RRGSTRLM KWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERANSRRGSTRLMNKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASF G+ALFDNGTSLLYFKGQKIWESIILDLLPKGL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFGGNALFDNGTSLLYFKGQKIWESIILDLLPKGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLA FLHCENF+KYLP NA+VKCLSDAGFFLDLRD GLNHTMRNFY+DLVALQGM+ N
Sbjct: 181 AGGLATFLHCENFSKYLPRNASVKCLSDAGFFLDLRDTGLNHTMRNFYRDLVALQGMQQN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LNE+CTA+SY+P+LCIFPQYSLKYITTPFF+LNSAYDV+QFHHGLVPPS+DL GHWNHCK
Sbjct: 241 LNEDCTAASYFPELCIFPQYSLKYITTPFFVLNSAYDVFQFHHGLVPPSADLRGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           L+PA CSEQQI VLQGLR+SMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWF E+SP
Sbjct: 301 LNPAECSEQQIQVLQGLRISMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFGEDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TID+KTIAEAVGDWYF  RRN KEIDCAYPCDS+CHNIIP
Sbjct: 361 TIDDKTIAEAVGDWYFG-RRNTKEIDCAYPCDSTCHNIIP 397

BLAST of MS000411 vs. ExPASy TrEMBL
Match: A0A1S3AYA0 (Pectin acetylesterase OS=Cucumis melo OX=3656 GN=LOC103484085 PE=3 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.0e-208
Identity = 350/400 (87.50%), Postives = 374/400 (93.50%), Query Frame = 0

Query: 1   MKPAAVEASLVALLLLASALRWPRGAEARLLVEMTLVRNASSIGALCLDGSLPAYHLHRG 60
           M P AVEA L+ L LLA+AL  P G  ARLLVEMT+VRNASSIGA CLDGSLPAYHL RG
Sbjct: 1   MSPTAVEAPLLLLFLLAAALLCPLGG-ARLLVEMTVVRNASSIGAFCLDGSLPAYHLDRG 60

Query: 61  FGAGAGNWLLQFEGGGWCNDVKSCLERASTRRGSTRLMTKWEDFSGILSNNASLNPADFY 120
           FGAGAGNWLLQFEGGGWCNDVKSC ERA++RRGST LMTKWEDFSGILSNNASLNP DFY
Sbjct: 61  FGAGAGNWLLQFEGGGWCNDVKSCFERANSRRGSTHLMTKWEDFSGILSNNASLNP-DFY 120

Query: 121 NWNRVKLRYCDGASFAGDALFDNGTSLLYFKGQKIWESIILDLLPKGLATAQKALLSGCS 180
           NWNRVKLRYCDGASFAGDALFDNGTS+L+FKGQK+WESIILDLLPKGL TA+KALLSGCS
Sbjct: 121 NWNRVKLRYCDGASFAGDALFDNGTSVLHFKGQKMWESIILDLLPKGLGTARKALLSGCS 180

Query: 181 AGGLAAFLHCENFTKYLPTNATVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVALQGMEPN 240
           AGGLA FLHCENFT YLP NA+VKCLSDAGFFLDLRDIGLNHTMRNFYKDLV+LQGME N
Sbjct: 181 AGGLATFLHCENFTNYLPRNASVKCLSDAGFFLDLRDIGLNHTMRNFYKDLVSLQGMEQN 240

Query: 241 LNENCTASSYYPDLCIFPQYSLKYITTPFFILNSAYDVYQFHHGLVPPSSDLHGHWNHCK 300
           LN+NCTASSY+P+LCIFPQYSLKYITTPFFILNSAYDV+QFHHGLVPPS+DLHGHWNHCK
Sbjct: 241 LNKNCTASSYFPELCIFPQYSLKYITTPFFILNSAYDVFQFHHGLVPPSADLHGHWNHCK 300

Query: 301 LDPAACSEQQIDVLQGLRMSMLSALRLFYEYSSRGGMFINSCFAHCQSESQDTWFAENSP 360
           L+PA CSE QI VLQGLR+SMLSALRLF+EYSSRGGMFINSCFAHCQSESQDTWFA +SP
Sbjct: 301 LNPAECSELQIQVLQGLRISMLSALRLFHEYSSRGGMFINSCFAHCQSESQDTWFAPDSP 360

Query: 361 TIDNKTIAEAVGDWYFSRRRNIKEIDCAYPCDSSCHNIIP 401
           TIDNKTIAE+VGDWYF  RRN+KEIDCAYPCDS+CHN+IP
Sbjct: 361 TIDNKTIAESVGDWYFG-RRNVKEIDCAYPCDSTCHNLIP 397

BLAST of MS000411 vs. TAIR 10
Match: AT5G23870.2 (Pectinacetylesterase family protein )

HSP 1 Score: 557.8 bits (1436), Expect = 7.3e-159
Identity = 254/370 (68.65%), Postives = 300/370 (81.08%), Query Frame = 0

Query: 32  VEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERASTR 91
           V MTLVR+A+++GA CLDGSLPAYHL RGFGAG+ NW+LQFEGGGWCND+ SC+ERA TR
Sbjct: 35  VSMTLVRDAAALGAFCLDGSLPAYHLDRGFGAGSNNWILQFEGGGWCNDIASCVERAKTR 94

Query: 92  RGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLYFK 151
           RGSTR M+K   F+G+LSNNAS NP DFYNWN+V+LRYCDGASFAGD+ F NGTSLLYF+
Sbjct: 95  RGSTRYMSKTVVFTGVLSNNASQNP-DFYNWNKVRLRYCDGASFAGDSQFGNGTSLLYFR 154

Query: 152 GQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDAGF 211
           GQ+IW +IILDLLPKGLA A KALL+GCSAGGL+ FLHC+NFT YLP NA+VKC+SDAGF
Sbjct: 155 GQRIWNAIILDLLPKGLAKAHKALLTGCSAGGLSTFLHCDNFTSYLPKNASVKCMSDAGF 214

Query: 212 FLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSY-YPDLCIFPQYSLKYITTPFF 271
           FLD  D+  N TMR+FY  LV+LQG++ NL+ +CT + +  P LC FPQY L++I TPFF
Sbjct: 215 FLDAIDVAANRTMRSFYSQLVSLQGIQKNLDPSCTHAFFPEPSLCFFPQYVLRFIKTPFF 274

Query: 272 ILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFYE 331
           ILNSAYDV+QFHHGLVPPS+D  G WN CKL+  AC+  Q+D LQG R  ML AL  F+ 
Sbjct: 275 ILNSAYDVFQFHHGLVPPSADQTGRWNRCKLNVTACNPHQLDALQGFRKDMLGALMNFFR 334

Query: 332 YSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAYP 391
            S+RGGMFINSCF HCQS  ++TW +  SP I+NKTIAE VGDWYF R    KEI C YP
Sbjct: 335 NSTRGGMFINSCFDHCQSALEETWLSPTSPRINNKTIAETVGDWYFGRGEEAKEIGCPYP 394

Query: 392 CDSSCHNIIP 401
           CD +CHN+IP
Sbjct: 395 CDKTCHNLIP 403

BLAST of MS000411 vs. TAIR 10
Match: AT5G23870.3 (Pectinacetylesterase family protein )

HSP 1 Score: 557.8 bits (1436), Expect = 7.3e-159
Identity = 254/370 (68.65%), Postives = 300/370 (81.08%), Query Frame = 0

Query: 32  VEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERASTR 91
           V MTLVR+A+++GA CLDGSLPAYHL RGFGAG+ NW+LQFEGGGWCND+ SC+ERA TR
Sbjct: 35  VSMTLVRDAAALGAFCLDGSLPAYHLDRGFGAGSNNWILQFEGGGWCNDIASCVERAKTR 94

Query: 92  RGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLYFK 151
           RGSTR M+K   F+G+LSNNAS NP DFYNWN+V+LRYCDGASFAGD+ F NGTSLLYF+
Sbjct: 95  RGSTRYMSKTVVFTGVLSNNASQNP-DFYNWNKVRLRYCDGASFAGDSQFGNGTSLLYFR 154

Query: 152 GQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDAGF 211
           GQ+IW +IILDLLPKGLA A KALL+GCSAGGL+ FLHC+NFT YLP NA+VKC+SDAGF
Sbjct: 155 GQRIWNAIILDLLPKGLAKAHKALLTGCSAGGLSTFLHCDNFTSYLPKNASVKCMSDAGF 214

Query: 212 FLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSY-YPDLCIFPQYSLKYITTPFF 271
           FLD  D+  N TMR+FY  LV+LQG++ NL+ +CT + +  P LC FPQY L++I TPFF
Sbjct: 215 FLDAIDVAANRTMRSFYSQLVSLQGIQKNLDPSCTHAFFPEPSLCFFPQYVLRFIKTPFF 274

Query: 272 ILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFYE 331
           ILNSAYDV+QFHHGLVPPS+D  G WN CKL+  AC+  Q+D LQG R  ML AL  F+ 
Sbjct: 275 ILNSAYDVFQFHHGLVPPSADQTGRWNRCKLNVTACNPHQLDALQGFRKDMLGALMNFFR 334

Query: 332 YSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAYP 391
            S+RGGMFINSCF HCQS  ++TW +  SP I+NKTIAE VGDWYF R    KEI C YP
Sbjct: 335 NSTRGGMFINSCFDHCQSALEETWLSPTSPRINNKTIAETVGDWYFGRGEEAKEIGCPYP 394

Query: 392 CDSSCHNIIP 401
           CD +CHN+IP
Sbjct: 395 CDKTCHNLIP 403

BLAST of MS000411 vs. TAIR 10
Match: AT5G23870.1 (Pectinacetylesterase family protein )

HSP 1 Score: 557.8 bits (1436), Expect = 7.3e-159
Identity = 254/370 (68.65%), Postives = 300/370 (81.08%), Query Frame = 0

Query: 32  VEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERASTR 91
           V MTLVR+A+++GA CLDGSLPAYHL RGFGAG+ NW+LQFEGGGWCND+ SC+ERA TR
Sbjct: 35  VSMTLVRDAAALGAFCLDGSLPAYHLDRGFGAGSNNWILQFEGGGWCNDIASCVERAKTR 94

Query: 92  RGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLYFK 151
           RGSTR M+K   F+G+LSNNAS NP DFYNWN+V+LRYCDGASFAGD+ F NGTSLLYF+
Sbjct: 95  RGSTRYMSKTVVFTGVLSNNASQNP-DFYNWNKVRLRYCDGASFAGDSQFGNGTSLLYFR 154

Query: 152 GQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDAGF 211
           GQ+IW +IILDLLPKGLA A KALL+GCSAGGL+ FLHC+NFT YLP NA+VKC+SDAGF
Sbjct: 155 GQRIWNAIILDLLPKGLAKAHKALLTGCSAGGLSTFLHCDNFTSYLPKNASVKCMSDAGF 214

Query: 212 FLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSY-YPDLCIFPQYSLKYITTPFF 271
           FLD  D+  N TMR+FY  LV+LQG++ NL+ +CT + +  P LC FPQY L++I TPFF
Sbjct: 215 FLDAIDVAANRTMRSFYSQLVSLQGIQKNLDPSCTHAFFPEPSLCFFPQYVLRFIKTPFF 274

Query: 272 ILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFYE 331
           ILNSAYDV+QFHHGLVPPS+D  G WN CKL+  AC+  Q+D LQG R  ML AL  F+ 
Sbjct: 275 ILNSAYDVFQFHHGLVPPSADQTGRWNRCKLNVTACNPHQLDALQGFRKDMLGALMNFFR 334

Query: 332 YSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAYP 391
            S+RGGMFINSCF HCQS  ++TW +  SP I+NKTIAE VGDWYF R    KEI C YP
Sbjct: 335 NSTRGGMFINSCFDHCQSALEETWLSPTSPRINNKTIAETVGDWYFGRGEEAKEIGCPYP 394

Query: 392 CDSSCHNIIP 401
           CD +CHN+IP
Sbjct: 395 CDKTCHNLIP 403

BLAST of MS000411 vs. TAIR 10
Match: AT2G46930.1 (Pectinacetylesterase family protein )

HSP 1 Score: 418.3 bits (1074), Expect = 6.9e-117
Identity = 197/370 (53.24%), Postives = 258/370 (69.73%), Query Frame = 0

Query: 30  LLVEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERAS 89
           L+V +TL++ A+S GA+CLDG+LP YHLH G G+GA  WL+Q EGGGWCN  +SC+ R +
Sbjct: 51  LMVPLTLIQAAASKGAVCLDGTLPGYHLHPGSGSGANRWLIQLEGGGWCNTRRSCIFRKT 110

Query: 90  TRRGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLY 149
           TRRGS+  M K   F+GILSN ++ NP DF+NWNRVKLRYCDGASF GD+   + +S LY
Sbjct: 111 TRRGSSNHMEKVLAFTGILSNKSNENP-DFFNWNRVKLRYCDGASFTGDS--QDESSQLY 170

Query: 150 FKGQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDA 209
           ++GQ+IW S + +LL KG+  A++ALLSGCSAGGLA+ LHC+ F +  P   TVKCLSDA
Sbjct: 171 YRGQRIWHSAMEELLSKGMQKAEQALLSGCSAGGLASILHCDQFKELFPGTTTVKCLSDA 230

Query: 210 GFFLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSYYPDLCIFPQYSLKYITTPF 269
           G F+D  D+   H++R  ++ +V +Q ++  L+  CT     P  C FPQ  +  I TP 
Sbjct: 231 GMFMDAVDVSGGHSLRKMFQGVVTVQNLQKELSTACT-KHLDPTSCFFPQNLVSGIKTPM 290

Query: 270 FILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFY 329
           F+LN+AYD +Q    L PPS DL G W  CK D + C+  QI   Q  R  M+ A++ F 
Sbjct: 291 FLLNAAYDAWQVQESLAPPSVDLSGSWKACKSDHSHCNSSQIQFFQDFRTHMVDAVKSF- 350

Query: 330 EYSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAY 389
             S+  G+FINSCFAHCQSE QDTW+A +SPT+  KT+AE+VGDWYF  R  +K IDC Y
Sbjct: 351 ATSTHNGVFINSCFAHCQSERQDTWYAPDSPTLHGKTVAESVGDWYFD-RTTVKAIDCPY 410

Query: 390 PCDSSCHNII 400
           PCD +CHN+I
Sbjct: 411 PCDKTCHNLI 414

BLAST of MS000411 vs. TAIR 10
Match: AT1G57590.1 (Pectinacetylesterase family protein )

HSP 1 Score: 417.9 bits (1073), Expect = 9.0e-117
Identity = 197/369 (53.39%), Postives = 261/369 (70.73%), Query Frame = 0

Query: 31  LVEMTLVRNASSIGALCLDGSLPAYHLHRGFGAGAGNWLLQFEGGGWCNDVKSCLERAST 90
           +V +TL+++A++ GA+CLDGSLP YHLHRGFG+GA NWL+Q EGGGWC+ +++C+ R +T
Sbjct: 79  MVGLTLIQSAAAKGAVCLDGSLPGYHLHRGFGSGANNWLVQLEGGGWCDTIRNCVYRKTT 138

Query: 91  RRGSTRLMTKWEDFSGILSNNASLNPADFYNWNRVKLRYCDGASFAGDALFDNGTSLLYF 150
           RRGS+  M K   F+GILS+ A+ NP DFYNWNRVK+RYCDG SF+GD+  +N  + L F
Sbjct: 139 RRGSSSYMEKEIPFTGILSDKAADNP-DFYNWNRVKVRYCDGGSFSGDS--ENKAAQLQF 198

Query: 151 KGQKIWESIILDLLPKGLATAQKALLSGCSAGGLAAFLHCENFTKYLPTNATVKCLSDAG 210
           +G++IW + + DL+ KG+  A++ALLSGCSAGGLA  L C++F K  P +  VKCLSDAG
Sbjct: 199 RGKRIWLAAMEDLMAKGMRQAKQALLSGCSAGGLAVILRCDDFGKLFPPSTRVKCLSDAG 258

Query: 211 FFLDLRDIGLNHTMRNFYKDLVALQGMEPNLNENCTASSYYPDLCIFPQYSLKYITTPFF 270
           FFLD  D+    ++R  Y  +V LQ ++ NL + C  +   P  C FPQ  +  + TP F
Sbjct: 259 FFLDAIDVSGGRSLRRLYAGVVRLQNLQTNLPQYC-VNRLNPTSCFFPQNLINQVKTPLF 318

Query: 271 ILNSAYDVYQFHHGLVPPSSDLHGHWNHCKLDPAACSEQQIDVLQGLRMSMLSALRLFYE 330
           ILN+AYD +Q    L P S+D  G WN C+L+ A CS  QI  LQG R  M++ ++ F  
Sbjct: 319 ILNAAYDSWQIQESLAPKSADPSGSWNDCRLNYAKCSASQIQFLQGFRTRMVNLVKGF-A 378

Query: 331 YSSRGGMFINSCFAHCQSESQDTWFAENSPTIDNKTIAEAVGDWYFSRRRNIKEIDCAYP 390
             S+ G+F+NSCFAHCQ+E  DTWFA+NSP I NK IA AVGDWYF  R   K IDCAYP
Sbjct: 379 MPSKNGVFLNSCFAHCQTERHDTWFAQNSPAIKNKGIAVAVGDWYF-ERGGAKLIDCAYP 438

Query: 391 CDSSCHNII 400
           CD +CHN++
Sbjct: 439 CDKTCHNLV 441