MS000410 (gene) Bitter gourd (TR) v1

BLAST of MS000410 vs. NCBI nr
Match: XP_022141037.1 (cytokinin hydroxylase [Momordica charantia])

HSP 1 Score: 1068.1 bits (2761), Expect = 2.4e-308
Identity = 529/532 (99.44%), Postives = 532/532 (100.00%), Query Frame = 0

Query: 3   MEWIGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIK 62
           MEWIGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIK
Sbjct: 1   MEWIGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIK 60

Query: 63  NTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPF 122
           NTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFA+WQTSYGKKFVYWLGTEPF
Sbjct: 61  NTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPF 120

Query: 123 LYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP 182
           LYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP
Sbjct: 121 LYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP 180

Query: 183 ANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRD 242
           ANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRD
Sbjct: 181 ANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRD 240

Query: 243 AFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDA 302
           AFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDA
Sbjct: 241 AFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDA 300

Query: 303 SASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATR 362
           SASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWT+LLLATR
Sbjct: 301 SASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATR 360

Query: 363 PEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD 422
           PEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD
Sbjct: 361 PEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD 420

Query: 423 TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMC 482
           TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMC
Sbjct: 421 TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMC 480

Query: 483 IGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           IGRNLSSMEYKIVLTLI+SRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL
Sbjct: 481 IGRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 532

BLAST of MS000410 vs. NCBI nr
Match: KAG6574181.1 (Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 812.0 bits (2096), Expect = 3.1e-231
Identity = 411/529 (77.69%), Postives = 455/529 (86.01%), Query Frame = 0

Query: 6   IGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTM 65
           IG I W    V ++VL V+VV SWW+LPVLAH+KLKRNGF  GPPP FPLGNI E+K   
Sbjct: 8   IGSITW----VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFR-GPPPSFPLGNIAEMKRIR 67

Query: 66  KKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYI 125
           K      A+AAING A +F  SS +SHDIHSTVFP+FA+WQ SYGKKF+YWLGTEPFLYI
Sbjct: 68  K------ASAAINGDAHSF-PSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYI 127

Query: 126 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 185
           A+P F+K +SEAVM KGWGKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANL
Sbjct: 128 ADPHFMKKISEAVMAKGWGKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANL 187

Query: 186 KAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFN 245
           KAMT+ MV STTKML++W+  ++S G E+DVEREITATAGDIIAKASFGIC+  GRDAF+
Sbjct: 188 KAMTKFMVESTTKMLNKWSSLVNSAGGEIDVEREITATAGDIIAKASFGICNDGGRDAFD 247

Query: 246 KLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS 305
            LR LQFTLF+NS+YVGVPFSS+F  P RTL+AKRLG+ IDRLFLS++S+RK S D S+S
Sbjct: 248 YLRALQFTLFQNSTYVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD-SSS 307

Query: 306 DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEW 365
           DLLSRLIE ++  S    GK  LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEW
Sbjct: 308 DLLSRLIEGEK--STGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEW 367

Query: 366 QTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 425
           Q ILREEIKEVIGD+EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG  TI
Sbjct: 368 QDILREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTI 427

Query: 426 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGR 485
           PKGTNIWIDIVAMH DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+
Sbjct: 428 PKGTNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGK 487

Query: 486 NLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           NLSSMEYKIVLTLILSRFSLS+SP+Y+HSPA LLSLRPAHGLPLI+ PL
Sbjct: 488 NLSSMEYKIVLTLILSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPL 521

BLAST of MS000410 vs. NCBI nr
Match: XP_022945852.1 (cytochrome P450 714C2-like [Cucurbita moschata])

HSP 1 Score: 810.4 bits (2092), Expect = 8.9e-231
Identity = 410/529 (77.50%), Postives = 455/529 (86.01%), Query Frame = 0

Query: 6   IGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTM 65
           IG I W    V ++VL V+VV SWW+LPVLAH+KLKRNGF  GPPP FPLGNI E+K   
Sbjct: 8   IGSITW----VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFR-GPPPSFPLGNIAEMKRIR 67

Query: 66  KKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYI 125
           K      A+AAING A +F  SS +SHDIHSTVFP+FA+WQ SYGKKF+YWLGTEPFLYI
Sbjct: 68  K------ASAAINGDAHSF-PSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYI 127

Query: 126 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 185
           A+P F+K +SEAVM KGWGKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANL
Sbjct: 128 ADPHFMKKISEAVMAKGWGKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANL 187

Query: 186 KAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFN 245
           KAMT+ MV STTKML++W+  ++S G E+DVEREITATAGDIIA+ASFGIC+  GRDAF+
Sbjct: 188 KAMTKFMVESTTKMLNKWSSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFD 247

Query: 246 KLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS 305
            LR LQFTLF+NS+YVGVPFSS+F  P RTL+AKRLG+ IDRLFLS++S+RK S D S+S
Sbjct: 248 YLRALQFTLFQNSTYVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD-SSS 307

Query: 306 DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEW 365
           DLLSRLIE ++  S    GK  LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEW
Sbjct: 308 DLLSRLIEGEK--STGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEW 367

Query: 366 QTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 425
           Q ILREEIKEVIGD+EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG  TI
Sbjct: 368 QDILREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTI 427

Query: 426 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGR 485
           PKGTNIWIDIVAMH DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+
Sbjct: 428 PKGTNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGK 487

Query: 486 NLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           NLSSMEYKIVLTLILSRFSLS+SP+Y+HSPA LLSLRPAHGLPLI+ PL
Sbjct: 488 NLSSMEYKIVLTLILSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPL 521

BLAST of MS000410 vs. NCBI nr
Match: XP_022968014.1 (cytochrome P450 714C2-like [Cucurbita maxima])

HSP 1 Score: 806.2 bits (2081), Expect = 1.7e-229
Identity = 410/529 (77.50%), Postives = 453/529 (85.63%), Query Frame = 0

Query: 6   IGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTM 65
           IG+I W    V ++VL V+VV SWW+LPVLAH+KLKRNGF  GPPP FPLGNI E+K   
Sbjct: 8   IGLITW----VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFR-GPPPTFPLGNIAEMKRIR 67

Query: 66  KKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYI 125
           K      A+AAING + +F  SS +SHDIHSTVFP+FA+WQ SYGKKFVYWLGTEPFLYI
Sbjct: 68  K------ASAAINGDSHSF-PSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYI 127

Query: 126 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 185
           A+P F+K +S AVM KGWGKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANL
Sbjct: 128 ADPHFMKQISVAVMAKGWGKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANL 187

Query: 186 KAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFN 245
           KAMT+ MV STTKML+EW+  ++S   E+DVEREITATAGDIIAKASFGIC+  GRDAF+
Sbjct: 188 KAMTKFMVESTTKMLNEWSSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFD 247

Query: 246 KLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS 305
            LR LQFTLF+NS+YVGVPFSS+F  P RTL AKRLG+ IDRLFLS++SERK S + S+S
Sbjct: 248 YLRALQFTLFQNSTYVGVPFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQN-SSS 307

Query: 306 DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEW 365
           DLLSRLIE Q+  S    GK  LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEW
Sbjct: 308 DLLSRLIEGQK--STGDVGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEW 367

Query: 366 QTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 425
           Q ILREEIKEVIGD+EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI+VG  TI
Sbjct: 368 QDILREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTI 427

Query: 426 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGR 485
           PKGTNIWIDIVAMH DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+
Sbjct: 428 PKGTNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGK 487

Query: 486 NLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           +LSSMEYKIVLTLILSRFSLS+SP+YSHSPA LLSLRPAHGLPLI+ PL
Sbjct: 488 SLSSMEYKIVLTLILSRFSLSLSPNYSHSPATLLSLRPAHGLPLIVTPL 521

BLAST of MS000410 vs. NCBI nr
Match: XP_038877847.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 806.2 bits (2081), Expect = 1.7e-229
Identity = 409/530 (77.17%), Postives = 452/530 (85.28%), Query Frame = 0

Query: 8   VIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKK 67
           +I+W  IR +++V   +VV SWWILP+LAH+ LKRNGF  GP P FPLGNITE+K  +K 
Sbjct: 10  LIMWA-IRGTIMVFFGKVVVSWWILPILAHKNLKRNGFE-GPSPTFPLGNITEMKKIIK- 69

Query: 68  AAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYIAE 127
                AAAAINGG+ + +SS+N+SHDIHST+FP+FA+WQ SYGKKFVYWLGTEPFLYIA+
Sbjct: 70  -----AAAAINGGS-SLASSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 129

Query: 128 PQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKA 187
           PQ VK +SEAVMGK WGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP NLKA
Sbjct: 130 PQLVKKISEAVMGKSWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKA 189

Query: 188 MTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICD-RSGRDAFNK 247
           MT+ M+ STTKM+ EW   ++SG  E+DVEREITATAGDIIAKASFGI     G +A N 
Sbjct: 190 MTKFMLESTTKMVKEWRSLVNSGQSEIDVEREITATAGDIIAKASFGISSGGGGEEALNS 249

Query: 248 LRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERK--QSPDASA 307
           LR LQ TLF NSSYVGVPFSS+FLYP RTL AKRLG++IDRLF SIIS RK   S   S 
Sbjct: 250 LRALQLTLFSNSSYVGVPFSSLFLYPGRTLAAKRLGAQIDRLFFSIISHRKLISSGSDSD 309

Query: 308 SDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPE 367
           SDLLSRLI+SQ N      G + LT RE+VDECKTFFFGGHETTALAISWTLLLLATRPE
Sbjct: 310 SDLLSRLIQSQSN---KYKGGAALTPREVVDECKTFFFGGHETTALAISWTLLLLATRPE 369

Query: 368 WQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTT 427
           WQ ILREEIKEVIGD++IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI +GD  
Sbjct: 370 WQKILREEIKEVIGDKDIDFSMLSALKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDMK 429

Query: 428 IPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIG 487
           IPKGTNIWIDIVAMHHDP+LWGDDVNEFKPERF+ D+IHGGC HKMGYLPFGFGGRMCIG
Sbjct: 430 IPKGTNIWIDIVAMHHDPTLWGDDVNEFKPERFQHDTIHGGCNHKMGYLPFGFGGRMCIG 489

Query: 488 RNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           RNLSS EYKIVLTLILS FSLS+SPSYSHSPAILLSLRP HG+PLIL PL
Sbjct: 490 RNLSSTEYKIVLTLILSTFSLSLSPSYSHSPAILLSLRPTHGIPLILTPL 527

BLAST of MS000410 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.2e-86
Identity = 188/534 (35.21%), Postives = 283/534 (53.00%), Query Frame = 0

Query: 16  VSLVVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAAS 75
           V  V  ++RV++     +W+ P    + +++ G   GP PR   GNI EI   + ++A+ 
Sbjct: 11  VIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVT-GPKPRPLTGNILEISAMVSQSAS- 70

Query: 76  AAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYIAEPQFV 135
                            +I HDI   + P++  W   YGK+F+ W GT+P L + E + +
Sbjct: 71  -------------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELI 130

Query: 136 KNM---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAM 195
           K +      V G+ W +    +N      G GL+M  G DW   RH+  PAF    LK  
Sbjct: 131 KELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGY 190

Query: 196 TRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLR 255
            R MV  T+K+++   + +  G  E+++  E+     DII++  FG     G++ FN L 
Sbjct: 191 ARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLT 250

Query: 256 DLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS--- 315
            LQ    + + ++  P  S FL        K L  E++RL + II  R+   +   S   
Sbjct: 251 VLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTH 310

Query: 316 --DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRP 375
             DLL  L+     D       + L  + ++DECKTFFF GHETTAL ++WT +LLA  P
Sbjct: 311 GDDLLGLLLNEMDIDKNNNNNNNNL--QLIMDECKTFFFAGHETTALLLTWTTMLLADNP 370

Query: 376 EWQTILREEIKEVIGDREI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD 435
            WQ  +REE++EV G   +     LS+L  +  V NE LRLYP A  + R A  D+K+GD
Sbjct: 371 TWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGD 430

Query: 436 TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMG--YLPFGFGGR 495
            TIPKG +IWI ++A+HH   LWG D N+F PERF      GG     G  ++PF  G R
Sbjct: 431 LTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPR 490

Query: 496 MCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
            CIG+  + ME KI+L  ++S+F+ +IS +Y H+P ++L+++P +G+ +ILKPL
Sbjct: 491 NCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of MS000410 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.5e-84
Identity = 191/539 (35.44%), Postives = 293/539 (54.36%), Query Frame = 0

Query: 9   IVWGIIRVSLVVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNT 68
           +V   + V ++ L++RV++     +++ P    + ++R G   GP PR   GNI +I   
Sbjct: 5   LVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGIT-GPKPRLLTGNIIDISKM 64

Query: 69  MKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLY 128
           +  +A+              +  S+I H+I   + P++  W   YGK+F+ W GTEP L 
Sbjct: 65  LSHSAS--------------NDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLC 124

Query: 129 IAEPQFVKNM---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFN 188
           + E + +K +      V GK W    + +   +   G GL+M  G+ W   RH+  PAF 
Sbjct: 125 LTETEMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFT 184

Query: 189 PANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGR 248
              LK   + MV   TKM+ E  R+    G E+++  E+     DII++  FG     G+
Sbjct: 185 RDRLKGYAKHMV-ECTKMMAERLRK--EVGEEVEIGEEMRRLTADIISRTEFGSSCDKGK 244

Query: 249 DAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD 308
           + F+ L  LQ    + + ++  P  S FL        K L +E++RL + II  RK S +
Sbjct: 245 ELFSLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVE 304

Query: 309 ASAS-----DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTL 368
              S     DLL  L+    ++      K+ L  + ++DECKTFFF GHETT+L ++WTL
Sbjct: 305 IGRSSSYGDDLLGLLLNQMDSN------KNNLNVQMIMDECKTFFFTGHETTSLLLTWTL 364

Query: 369 LLLATRPEWQTILREEIKEVIG-DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAK 428
           +LLA  P WQ  +R+E+++V G D       LS L  +  V NE LRLYP A  + R A 
Sbjct: 365 MLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAF 424

Query: 429 GDIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPF 488
            DIK+GD  IPKG +IWI ++A+HH   LWG+D NEF PERF   S     +H   ++PF
Sbjct: 425 EDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPF 484

Query: 489 GFGGRMCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
             G R CIG+  + ME KI+L +++S+FS +IS +Y H+P ++L+++P +G+ L+LKPL
Sbjct: 485 AAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of MS000410 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 5.9e-84
Identity = 187/522 (35.82%), Postives = 278/522 (53.26%), Query Frame = 0

Query: 19  VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN 78
           ++L   V    W+ P    +KL+  G   GP P F  GNI E++   + A ++    A  
Sbjct: 20  ILLFSYVYIILWLRPERLRQKLRSQGVR-GPKPSFLFGNIPEMRRIQQLAKSAHEQEA-- 79

Query: 79  GGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV 138
           G    FSS      +  +T+FPYF  W   YG  ++Y  G+   L + +P  VK ++   
Sbjct: 80  GSTDMFSS------NYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANC- 139

Query: 139 MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK 198
                GKP   + +R  + G G++ + GD W+  R ++ P      +K M  LM+ +   
Sbjct: 140 KSLDLGKPCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMS 199

Query: 199 MLDEWARRLS--SGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFR 258
           ML+ W   +    G  E+ V+  +   + D+I++A FG     G++ F K+R LQ  + +
Sbjct: 200 MLNSWKNEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAK 259

Query: 259 NSSYVGVPFSSIFLYPTRTLTA-KRLGSEIDRLFLSIISERKQSPDASAS---DLLSRLI 318
            S  +GVP S     PTR+      L S I  L L+I   +K   D+S S   DLL  +I
Sbjct: 260 QSMLIGVPGSRYL--PTRSNRGIWNLDSSIRTLILNI--SKKYEHDSSTSVNKDLLHSII 319

Query: 319 ESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQTILREE 378
           +  ++   P A  S      +VD CK  +F GHETT+   +W L+LLA+  EWQ+  R E
Sbjct: 320 QGSKD--GPFA--SCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVE 379

Query: 379 IKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIW 438
             ++   R +DF +L +LKK+  V  E LRLYP A  + R+A  D+K+G   IPKGTNIW
Sbjct: 380 SLDICQGRPLDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIW 439

Query: 439 IDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEY 498
           I I   H DPS+WG   ++F P+RF  + I G CK    Y+PFG G R C G+NL+ +E 
Sbjct: 440 IPIAMAHRDPSVWGPSADKFDPDRF-ANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVEL 499

Query: 499 KIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           K+VL+L+LS+F   +SP+Y H PA  L++ P  G+PLI + L
Sbjct: 500 KVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

BLAST of MS000410 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.2e-82
Identity = 173/507 (34.12%), Postives = 268/507 (52.86%), Query Frame = 0

Query: 30  WILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAINGGAGAFSSSSN 89
           W++P     K +     GGP P FP GN+ +++     A A+ A+A  +G  G       
Sbjct: 32  WLVPQKVLAKFEDQKI-GGPRPSFPYGNLADMREAAAAAKAARASARRSGSGG-----GG 91

Query: 90  ISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVMGKGWGKPAVF 149
           I HD    V PY+ KW+  YG  F Y +G   FL+++ P  V++++  V     GK +  
Sbjct: 92  IVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYL 151

Query: 150 RNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKMLDEWARRLSS 209
           +   EP+FG G++ + G+ W+  R I+ P F    +K M  LMV S   +L  W  R+  
Sbjct: 152 KATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDR 211

Query: 210 GG--PEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSSYVGVPFSS 269
            G   ++ ++ +I A + D+I++  FG     G++ F K+R+LQ  + + +  V    + 
Sbjct: 212 NGGITDIKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAVSKPN--VLAEMTG 271

Query: 270 IFLYPT-RTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDSAPAAGKS 329
           +  +P+ R   A  L  ++ +L L I+ E  +       +LLS ++ S        A   
Sbjct: 272 LRFFPSMRNKQAWELHKQVRKLILEIVKESGED-----RNLLSAILHSASTSRVGIAE-- 331

Query: 330 WLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQTILREEIKEVIGDREIDFAM 389
                 +VD CK+ +F GHE+TA+  +W L+LL   PEWQ  +REE+ EV   + +D   
Sbjct: 332 --AENFIVDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRS 391

Query: 390 LSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMHHDPSLWG 449
           L ++K +  V  E LRLYP+   + RQA  ++K+G   IPKG NI+I +  MH DP LWG
Sbjct: 392 LQKMKNLTMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWG 451

Query: 450 DDVNEFKPERFE--KDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 509
            DV EF PERF   +  +H        YLPFG G R C+G+  +  E KI+++LI+S+F 
Sbjct: 452 PDVKEFNPERFSDVRPQLH-------SYLPFGAGARTCLGQGFAMAELKILISLIVSKFV 511

Query: 510 LSISPSYSHSPAILLSLRPAHGLPLIL 532
           L +SP Y HSP + L + P  G+ L L
Sbjct: 512 LKLSPHYQHSPTLKLIVEPELGVDLTL 513

BLAST of MS000410 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.1e-81
Identity = 175/552 (31.70%), Postives = 282/552 (51.09%), Query Frame = 0

Query: 13  IIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASA 72
           ++  + V+  + +  +W   P       +R G +G PP  F  GN+ E+K  +  AA++A
Sbjct: 15  VVVAAAVIAGLCITAAWLARPRRVAEVFRRQGIDGPPPSSFLAGNLPEMKARVAAAASAA 74

Query: 73  A-------AAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYI 132
           A        A+  GG G      +   D  + +FPYF KW+ +YG+ ++YWL   P LY+
Sbjct: 75  APTADGEETASAGGGGGGRDFEKDGFDDYCTRIFPYFHKWRKAYGETYLYWLRRRPALYV 134

Query: 133 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 192
            +P+ +  +   V     GKP   +  +EP+FG G++   G  W R R ++ P F  A +
Sbjct: 135 TDPELIGEIGRCV-SLDMGKPKYLQKGQEPLFGGGVLKANGACWARQRKVIAPEFYMARV 194

Query: 193 KAMTRLMVGSTTKMLDEWARRLSSGG----PEMDVEREITATAGDIIAKASFGICDRSGR 252
           +AM +LMV +   ++  W  R+ + G     E+ V+ ++ + + D+I++A FG     GR
Sbjct: 195 RAMVQLMVDAAQPLIASWESRIDAAGGAAAAEVVVDGDLRSFSFDVISRACFGSDYSRGR 254

Query: 253 DAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPT-RTLTAKRLGSEIDRLFLSIISERK--- 312
           + F +LR+L   +   S    +P  S+   PT +     RL  EI  L + ++ ER+   
Sbjct: 255 EIFLRLRELSGLMSETSVIFSIP--SLRHLPTGKNRRIWRLTGEIRSLIMELVRERRCAA 314

Query: 313 ----------QSPDASASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHET 372
                       P     D L  +IE+      P           +VD CK  +F GHET
Sbjct: 315 RAAREHGGKAAPPSPPERDFLGSIIENSGGQPRP--------DDFVVDNCKNIYFAGHET 374

Query: 373 TALAISWTLLLLATRPEWQTILREEIKEVIG------DREIDFAMLSRLKKMGWVFNEVL 432
           +A+  +W L+LLA  PEWQ   R E+ EV G          DF M+SR++ +G V  E L
Sbjct: 375 SAVTATWCLMLLAAHPEWQDRARAEVLEVCGGDGAAAPAAPDFDMVSRMRTVGMVVQETL 434

Query: 433 RLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDS 492
           RL+P +  + R+   D+++G    PKGT +++ +  MHHD + WG     F P RF +D 
Sbjct: 435 RLFPPSSFVVRETFRDMQLGRLLAPKGTYLFVPVSTMHHDVAAWGPTARLFDPSRF-RDG 494

Query: 493 IHGGCKH-KMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLS 533
           +   CKH +  ++PFG G R C+G+NL+ +E K ++ ++L+RF  ++SP Y HSPA  L 
Sbjct: 495 VAAACKHPQASFMPFGLGARTCLGQNLALVEVKTLVAVVLARFEFTLSPEYRHSPAFRLI 554

BLAST of MS000410 vs. ExPASy TrEMBL
Match: A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)

HSP 1 Score: 1068.1 bits (2761), Expect = 1.2e-308
Identity = 529/532 (99.44%), Postives = 532/532 (100.00%), Query Frame = 0

Query: 3   MEWIGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIK 62
           MEWIGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIK
Sbjct: 1   MEWIGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIK 60

Query: 63  NTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPF 122
           NTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFA+WQTSYGKKFVYWLGTEPF
Sbjct: 61  NTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPF 120

Query: 123 LYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP 182
           LYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP
Sbjct: 121 LYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP 180

Query: 183 ANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRD 242
           ANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRD
Sbjct: 181 ANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRD 240

Query: 243 AFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDA 302
           AFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDA
Sbjct: 241 AFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDA 300

Query: 303 SASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATR 362
           SASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWT+LLLATR
Sbjct: 301 SASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATR 360

Query: 363 PEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD 422
           PEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD
Sbjct: 361 PEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD 420

Query: 423 TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMC 482
           TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMC
Sbjct: 421 TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMC 480

Query: 483 IGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           IGRNLSSMEYKIVLTLI+SRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL
Sbjct: 481 IGRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 532

BLAST of MS000410 vs. ExPASy TrEMBL
Match: A0A6J1G224 (cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 4.3e-231
Identity = 410/529 (77.50%), Postives = 455/529 (86.01%), Query Frame = 0

Query: 6   IGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTM 65
           IG I W    V ++VL V+VV SWW+LPVLAH+KLKRNGF  GPPP FPLGNI E+K   
Sbjct: 8   IGSITW----VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFR-GPPPSFPLGNIAEMKRIR 67

Query: 66  KKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYI 125
           K      A+AAING A +F  SS +SHDIHSTVFP+FA+WQ SYGKKF+YWLGTEPFLYI
Sbjct: 68  K------ASAAINGDAHSF-PSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYI 127

Query: 126 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 185
           A+P F+K +SEAVM KGWGKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANL
Sbjct: 128 ADPHFMKKISEAVMAKGWGKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANL 187

Query: 186 KAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFN 245
           KAMT+ MV STTKML++W+  ++S G E+DVEREITATAGDIIA+ASFGIC+  GRDAF+
Sbjct: 188 KAMTKFMVESTTKMLNKWSSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFD 247

Query: 246 KLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS 305
            LR LQFTLF+NS+YVGVPFSS+F  P RTL+AKRLG+ IDRLFLS++S+RK S D S+S
Sbjct: 248 YLRALQFTLFQNSTYVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD-SSS 307

Query: 306 DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEW 365
           DLLSRLIE ++  S    GK  LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEW
Sbjct: 308 DLLSRLIEGEK--STGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEW 367

Query: 366 QTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 425
           Q ILREEIKEVIGD+EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG  TI
Sbjct: 368 QDILREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTI 427

Query: 426 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGR 485
           PKGTNIWIDIVAMH DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+
Sbjct: 428 PKGTNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGK 487

Query: 486 NLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           NLSSMEYKIVLTLILSRFSLS+SP+Y+HSPA LLSLRPAHGLPLI+ PL
Sbjct: 488 NLSSMEYKIVLTLILSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPL 521

BLAST of MS000410 vs. ExPASy TrEMBL
Match: A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 8.2e-230
Identity = 410/529 (77.50%), Postives = 453/529 (85.63%), Query Frame = 0

Query: 6   IGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTM 65
           IG+I W    V ++VL V+VV SWW+LPVLAH+KLKRNGF  GPPP FPLGNI E+K   
Sbjct: 8   IGLITW----VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFR-GPPPTFPLGNIAEMKRIR 67

Query: 66  KKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYI 125
           K      A+AAING + +F  SS +SHDIHSTVFP+FA+WQ SYGKKFVYWLGTEPFLYI
Sbjct: 68  K------ASAAINGDSHSF-PSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYI 127

Query: 126 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 185
           A+P F+K +S AVM KGWGKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANL
Sbjct: 128 ADPHFMKQISVAVMAKGWGKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANL 187

Query: 186 KAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFN 245
           KAMT+ MV STTKML+EW+  ++S   E+DVEREITATAGDIIAKASFGIC+  GRDAF+
Sbjct: 188 KAMTKFMVESTTKMLNEWSSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFD 247

Query: 246 KLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS 305
            LR LQFTLF+NS+YVGVPFSS+F  P RTL AKRLG+ IDRLFLS++SERK S + S+S
Sbjct: 248 YLRALQFTLFQNSTYVGVPFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQN-SSS 307

Query: 306 DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEW 365
           DLLSRLIE Q+  S    GK  LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEW
Sbjct: 308 DLLSRLIEGQK--STGDVGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEW 367

Query: 366 QTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 425
           Q ILREEIKEVIGD+EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI+VG  TI
Sbjct: 368 QDILREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTI 427

Query: 426 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGR 485
           PKGTNIWIDIVAMH DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+
Sbjct: 428 PKGTNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGK 487

Query: 486 NLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           +LSSMEYKIVLTLILSRFSLS+SP+YSHSPA LLSLRPAHGLPLI+ PL
Sbjct: 488 SLSSMEYKIVLTLILSRFSLSLSPNYSHSPATLLSLRPAHGLPLIVTPL 521

BLAST of MS000410 vs. ExPASy TrEMBL
Match: A0A1S3AXX2 (cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 7.1e-226
Identity = 404/531 (76.08%), Postives = 451/531 (84.93%), Query Frame = 0

Query: 6   IGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTM 65
           I +I W +IRVS++VL  +VV S WILP+LAHRKLKRNGF  GP P FPLGNITE+K  M
Sbjct: 8   IMLIKW-MIRVSIMVLFGKVVISCWILPILAHRKLKRNGFE-GPSPSFPLGNITEMKKIM 67

Query: 66  KKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYI 125
           K  AA+AA A  NG     SSS+N+SHDIHST+FP+FA+WQ SYGKKFVYWLGTEPFLYI
Sbjct: 68  K--AAAAATAVTNG-----SSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYI 127

Query: 126 AEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANL 185
           A+PQ VK +SEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NL
Sbjct: 128 ADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNL 187

Query: 186 KAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICD-RSGRDAF 245
           KAMT+ MV STTKML+EW   + SG  E++VER+ITATAG+IIAKASFGI +   G +A 
Sbjct: 188 KAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEAL 247

Query: 246 NKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASA 305
           N LR LQ TLF+NSSYVGVPFSS+FLYP RTL AKRLG++IDRLFLSIIS RK S   S 
Sbjct: 248 NNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSG 307

Query: 306 -SDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRP 365
            SDLLSRLI S+        G   L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P
Sbjct: 308 YSDLLSRLIGSE------YTGGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSP 367

Query: 366 EWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDT 425
           +WQ ILREEIKEV+GD++IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI +GD 
Sbjct: 368 DWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDI 427

Query: 426 TIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCI 485
            IPKGTNIWID+VAMHHDP+LWGDDVN+F P RF+ D+IHGGC HKMGYLPFGFGGRMCI
Sbjct: 428 KIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCI 487

Query: 486 GRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           GRNLSSMEYKIVLTLILS FSLS+SPSYSHSPA LLSLRPAHG+PLIL PL
Sbjct: 488 GRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPL 523

BLAST of MS000410 vs. ExPASy TrEMBL
Match: A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 2.6e-207
Identity = 366/476 (76.89%), Postives = 407/476 (85.50%), Query Frame = 0

Query: 61  IKNTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTE 120
           +K  MK  AA+AA A  NG     SSS+N+SHDIHST+FP+FA+WQ SYGKKFVYWLGTE
Sbjct: 1   MKKIMK--AAAAATAVTNG-----SSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTE 60

Query: 121 PFLYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAF 180
           PFLYIA+PQ VK +SEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAF
Sbjct: 61  PFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAF 120

Query: 181 NPANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICD-RS 240
           NP NLKAMT+ MV STTKML+EW   + SG  E++VER+ITATAG+IIAKASFGI +   
Sbjct: 121 NPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGG 180

Query: 241 GRDAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQS 300
           G +A N LR LQ TLF+NSSYVGVPFSS+FLYP RTL AKRLG++IDRLFLSIIS RK S
Sbjct: 181 GGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMS 240

Query: 301 PDASA-SDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLL 360
              S  SDLLSRLI S+        G   L+ RE+VDECKTFFFGGHETTALAISWTLLL
Sbjct: 241 GSGSGYSDLLSRLIGSE------YTGGEALSPREVVDECKTFFFGGHETTALAISWTLLL 300

Query: 361 LATRPEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDI 420
           LAT P+WQ ILREEIKEV+GD++IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI
Sbjct: 301 LATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDI 360

Query: 421 KVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFG 480
            +GD  IPKGTNIWID+VAMHHDP+LWGDDVN+F P RF+ D+IHGGC HKMGYLPFGFG
Sbjct: 361 DLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFG 420

Query: 481 GRMCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           GRMCIGRNLSSMEYKIVLTLILS FSLS+SPSYSHSPA LLSLRPAHG+PLIL PL
Sbjct: 421 GRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPL 463

BLAST of MS000410 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 609.4 bits (1570), Expect = 2.8e-174
Identity = 306/536 (57.09%), Postives = 383/536 (71.46%), Query Frame = 0

Query: 2   EMEWIGVIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEI 61
           EM  +  I + +  V + ++ +++    WI PV A +KL+ NGF  GP P FP GN+   
Sbjct: 5   EMSRVWYIFFKVFVVVICLMFLKLFLRCWIWPVRAQKKLRGNGF-VGPAPSFPFGNL--- 64

Query: 62  KNTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEP 121
            N MKK     A+  ++       SS+ I+HDIHS   P+FA+WQ  YGK FVYWLG EP
Sbjct: 65  -NDMKK--LKMASVVVDNS----KSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEP 124

Query: 122 FLYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFN 181
           F+Y+A+P+F+  MS+ V+GK WGKP VF+ DREPMFG GLVM EGDDW RHRHI+TPAF 
Sbjct: 125 FVYVADPEFLSVMSKGVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFA 184

Query: 182 PANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGR 241
           P NLK MT +MV S + MLD W  +++SG PE D+E EI  TAG+IIAK SFG+   +G 
Sbjct: 185 PLNLKVMTNMMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGT 244

Query: 242 DAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQS-- 301
                LR +QF LF ++ YVGVPFS+I  Y  +T+ AK LG EID L LS I++RK S  
Sbjct: 245 QVLKNLRAVQFALFNSNRYVGVPFSNILSY-KQTVKAKGLGHEIDGLLLSFINKRKISLA 304

Query: 302 -PDASASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLL 361
             D    DLL  L+++ +        K   T +ELVDECKTFFF GHETTALA++WT +L
Sbjct: 305 EGDDQGHDLLGMLLKADQ--------KGNFTAKELVDECKTFFFAGHETTALALTWTFML 364

Query: 362 LATRPEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDI 421
           LA  PEWQ  +REEI+EVIGD +I++  L+ LKKM WV NEVLRLYP APN QRQA+ DI
Sbjct: 365 LAIHPEWQDTIREEIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDI 424

Query: 422 KVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFG 481
           +V    IP GTNIWID+VAMHHD  LWGDDVNEFKPERF+  ++HGGCK+KMGY+PFGFG
Sbjct: 425 EVNGRVIPNGTNIWIDVVAMHHDVELWGDDVNEFKPERFD-GNLHGGCKNKMGYMPFGFG 484

Query: 482 GRMCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
           GRMCIGRNL++MEYKIVL+L+LSRF +S+SP Y HSP  +LSLRP +GLPLI++PL
Sbjct: 485 GRMCIGRNLTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

BLAST of MS000410 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 321.2 bits (822), Expect = 1.5e-87
Identity = 188/534 (35.21%), Postives = 283/534 (53.00%), Query Frame = 0

Query: 16  VSLVVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAAS 75
           V  V  ++RV++     +W+ P    + +++ G   GP PR   GNI EI   + ++A+ 
Sbjct: 11  VIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVT-GPKPRPLTGNILEISAMVSQSAS- 70

Query: 76  AAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYIAEPQFV 135
                            +I HDI   + P++  W   YGK+F+ W GT+P L + E + +
Sbjct: 71  -------------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELI 130

Query: 136 KNM---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAM 195
           K +      V G+ W +    +N      G GL+M  G DW   RH+  PAF    LK  
Sbjct: 131 KELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGY 190

Query: 196 TRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLR 255
            R MV  T+K+++   + +  G  E+++  E+     DII++  FG     G++ FN L 
Sbjct: 191 ARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLT 250

Query: 256 DLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS--- 315
            LQ    + + ++  P  S FL        K L  E++RL + II  R+   +   S   
Sbjct: 251 VLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTH 310

Query: 316 --DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLLLLATRP 375
             DLL  L+     D       + L  + ++DECKTFFF GHETTAL ++WT +LLA  P
Sbjct: 311 GDDLLGLLLNEMDIDKNNNNNNNNL--QLIMDECKTFFFAGHETTALLLTWTTMLLADNP 370

Query: 376 EWQTILREEIKEVIGDREI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD 435
            WQ  +REE++EV G   +     LS+L  +  V NE LRLYP A  + R A  D+K+GD
Sbjct: 371 TWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGD 430

Query: 436 TTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMG--YLPFGFGGR 495
            TIPKG +IWI ++A+HH   LWG D N+F PERF      GG     G  ++PF  G R
Sbjct: 431 LTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPR 490

Query: 496 MCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
            CIG+  + ME KI+L  ++S+F+ +IS +Y H+P ++L+++P +G+ +ILKPL
Sbjct: 491 NCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of MS000410 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 315.1 bits (806), Expect = 1.1e-85
Identity = 191/539 (35.44%), Postives = 293/539 (54.36%), Query Frame = 0

Query: 9   IVWGIIRVSLVVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNT 68
           +V   + V ++ L++RV++     +++ P    + ++R G   GP PR   GNI +I   
Sbjct: 5   LVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGIT-GPKPRLLTGNIIDISKM 64

Query: 69  MKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLY 128
           +  +A+              +  S+I H+I   + P++  W   YGK+F+ W GTEP L 
Sbjct: 65  LSHSAS--------------NDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLC 124

Query: 129 IAEPQFVKNM---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFN 188
           + E + +K +      V GK W    + +   +   G GL+M  G+ W   RH+  PAF 
Sbjct: 125 LTETEMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFT 184

Query: 189 PANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGR 248
              LK   + MV   TKM+ E  R+    G E+++  E+     DII++  FG     G+
Sbjct: 185 RDRLKGYAKHMV-ECTKMMAERLRK--EVGEEVEIGEEMRRLTADIISRTEFGSSCDKGK 244

Query: 249 DAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD 308
           + F+ L  LQ    + + ++  P  S FL        K L +E++RL + II  RK S +
Sbjct: 245 ELFSLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVE 304

Query: 309 ASAS-----DLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTL 368
              S     DLL  L+    ++      K+ L  + ++DECKTFFF GHETT+L ++WTL
Sbjct: 305 IGRSSSYGDDLLGLLLNQMDSN------KNNLNVQMIMDECKTFFFTGHETTSLLLTWTL 364

Query: 369 LLLATRPEWQTILREEIKEVIG-DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAK 428
           +LLA  P WQ  +R+E+++V G D       LS L  +  V NE LRLYP A  + R A 
Sbjct: 365 MLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAF 424

Query: 429 GDIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPF 488
            DIK+GD  IPKG +IWI ++A+HH   LWG+D NEF PERF   S     +H   ++PF
Sbjct: 425 EDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPF 484

Query: 489 GFGGRMCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 535
             G R CIG+  + ME KI+L +++S+FS +IS +Y H+P ++L+++P +G+ L+LKPL
Sbjct: 485 AAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of MS000410 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 298.9 bits (764), Expect = 8.1e-81
Identity = 176/530 (33.21%), Postives = 269/530 (50.75%), Query Frame = 0

Query: 8   VIVWGIIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKK 67
           +++ GI  V   V    VV  W +      R LK  G   GPPP    GN++E++     
Sbjct: 15  IVIVGIFSVGYHVYGRAVVEQWRM-----RRSLKLQGVK-GPPPSIFNGNVSEMQR---- 74

Query: 68  AAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYIAE 127
                    I   A   S  + ISHD  S++FP+F  W+  YG+ + Y  G +  LYI  
Sbjct: 75  ---------IQSEAKHCSGDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINH 134

Query: 128 PQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKA 187
           P+ VK +S+       G+         P+ G+G++ + G  W   R I+   F    +K 
Sbjct: 135 PEMVKELSQ-TNTLNLGRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKG 194

Query: 188 MTRLMVGSTTKMLDEWARRLSSGGP---EMDVEREITATAGDIIAKASFGICDRSGRDAF 247
           M  LMV S   ML++W   +  GG    ++ V+ ++   + D+IAKA FG     G+  F
Sbjct: 195 MVGLMVESAMPMLNKWEEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIF 254

Query: 248 NKLRDL------QFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERK- 307
           + +RDL      +  LFR + +  + F S        +    L  E++      + ER+ 
Sbjct: 255 SMIRDLLTAITKRSVLFRFNGFTDMVFGS---KKHGDVDIDALEMELESSIWETVKEREI 314

Query: 308 QSPDASASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTLL 367
           +  D    DL+  ++E                 R +VD CK+ +F GH++TA+++SW L+
Sbjct: 315 ECKDTHKKDLMQLILEGAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLM 374

Query: 368 LLATRPEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGD 427
           LLA  P WQ  +R+EI     +   D   +  LK +  V  E +RLYP AP + R+A  D
Sbjct: 375 LLALNPSWQVKIRDEILSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKD 434

Query: 428 IKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGF 487
           I++GD  +PKG  IW  I A+H DP +WG D N+FKPERF  + I   CK+   Y+PFG 
Sbjct: 435 IRLGDLVVPKGVCIWTLIPALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPFGL 494

Query: 488 GGRMCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGL 528
           G R C+G+N   ME K++++LI+S+FS ++SP+Y HSP+  L + P HG+
Sbjct: 495 GPRTCVGKNFGMMEVKVLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGV 520

BLAST of MS000410 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 2.0e-79
Identity = 174/544 (31.99%), Postives = 288/544 (52.94%), Query Frame = 0

Query: 13  IIRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASA 72
           ++ V L +++V+ +   W  P        + G   GPP  F +GN+ E+   M KA+   
Sbjct: 15  VLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIR-GPPYHFFIGNVKELVGMMLKAS--- 74

Query: 73  AAAAINGGAGAFSSSSNISHDIHSTVFPYFAKWQTSYGKKFVYWLGTEPFLYIAEPQFVK 132
                       S     SH+I   V  ++  W+  YG  F+ W G    L +A+P  ++
Sbjct: 75  ------------SHPMPFSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIR 134

Query: 133 NMSEAVMGKGWGKPAVF-RNDREPMF----GDGLVMTEGDDWIRHRHILTPAFNPANLKA 192
            +        + K   + +N+  P+     GDGL+  +G+ W  HR I++P F+  NLK 
Sbjct: 135 EI--------FSKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKL 194

Query: 193 MTRLMVGSTTKMLDEWARRLSSGGP-EMDVEREITATAGDIIAKASFGICDRSGRDAFNK 252
           +  +++ S T M+D+W+ +LS  G  E+DV         D+I++ +FG     GR  F +
Sbjct: 195 LVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVF-R 254

Query: 253 LRDLQFTLFRNSSYVGVPFSSIFL-----YPTR-TLTAKRLGSEIDRLFLSIISERKQS- 312
           L+  Q  L   +      F  +F+     +PTR  L + +L  EI +  L +I  R+Q+ 
Sbjct: 255 LQAQQMLLCAEA------FQKVFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNA 314

Query: 313 --------PDASASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALA 372
                    + +A DLL  +I+++            +T +++V+ECK+FFF G +TT+  
Sbjct: 315 IDGEGEECKEPAAKDLLGLMIQAKN-----------VTVQDIVEECKSFFFAGKQTTSNL 374

Query: 373 ISWTLLLLATRPEWQTILREEIKEVIGDREIDFA-MLSRLKKMGWVFNEVLRLYPSAPNI 432
           ++WT +LL+  PEWQ   R+E+  V G R++     + +LK +  + NE LRLYP     
Sbjct: 375 LTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVAT 434

Query: 433 QRQAKGDIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKM 492
            R+AK D+K+G   IP GT + I I+A+HHD ++WG+DVNEF P RF  D +    KH +
Sbjct: 435 IRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARF-ADGVPRAAKHPV 494

Query: 493 GYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFSLSISPSYSHSPAILLSLRPAHGLPLI 535
           G++PFG G R CIG+NL+ ++ K+ L +++ RF+  ++P+Y H+P +L+ L P HG P+ 
Sbjct: 495 GFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPIT 515