MS000399 (gene) Bitter gourd (TR) v1

Overview


Name	MS000399
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	25.3 kDa vesicle transport protein
Location	scaffold44: 1446398 .. 1448159 (-)
RNA-Seq Expression	MS000399
Synteny	MS000399

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGTTGGAAGGCTTAGGGATGGGCTGCCACTTGCACAGGGCATTGGGTATTTTAATGAAGAGAATGATAGATGTTTTAGGCTACAAACAGCAAGCTGAATTCATACTCAAACACATCTCTAGAGGGGCCTTCTCACCTTCAAAAATGGCCATTCTGATTCATCATCAATGTTTCATGTACCACTCTTCAATTTTCTCTTTTCCTTTATTCTAACTCTTATTATTCATGATGAAGTTTAGAAAAAGAATTAATCCGGATTGTCCAATTTAGTTCTTCAACTTTTAGTCGAATGGATTATGCATCTCAAGAGAAAGAATGGGAGAGAATTGATGACGAAGAAATCACTTTTTCTCAAGCTTTTATTTTAAAAATGAAAACTTTAACACATACTTTAACATAAAAAATAAGATTGAAACAAATATTGTCAGGAGGTGTTTGGTTTATGAGTTAGGTTTGTTTAACTCAGGCTAAGAAATGGAGACTTAGATCTACAAAACTTTCTCTAGCATATAGGTTTTGCTTATTTCAGGTTACGATTTCCCACCTACTATATAGGTTAAGTTCGCGTTATATAAACTTAGATTTATAAAACTCACATTTTATACCATATTTTGCTCAATCAATCAAAGCATCACCCTGTCAAGGTGTTTACAGATTCGAGCTCCGTCAGTCCCGACGGATCAAAATCCTTGCAAAACTACAAATCAAACATTCTTATTTCTTCGTAACCTCAAAACTTATTAAGACAAAACAAAGTAGAGCTGCTTTTGTTGTCTCAGACTTTGAATTAAAGTTGGGTTTTTTGCATTTAGTTACTTGGTGGAGAATGGTGTCTGCTGCATCACATTGTGCGATTCTTCATACCCAAGAAAGCTGGCTCTCCATTACTTGCAAGATTTGCAAAAGGAGCTTGCCAAGTTTGATGATGCCCTAATAAACGTCTTACCAAACCCAATAGTTTTGCCAAATTTGGTAAGCCTTTCTCAACCCTCTTCATACAAATTTAGTTTCTTTCCAAATGTGTACGACAAGGAAATATTTTTTCACATCCTATACTACACATTATTAATAGTTTAATCAGTGAGTATTACTTCTTTTTATGATTTGAATTGAAATATACTTATTGACCAATTGATGTATGTAGATAGAATAATTAGCAATATTAGAAAACGGTACATTGATACAAGGACACAAGCCAATCTACATAAGCTCAATGCAGATCCTGGCTAAGGTGTCCAGACATTGTCACATGTTCACTCTTTGAAATTTTAGAAAGGAAAAAACTAGGTAATTATCGGCTCCTCATACTACACCAAAAGTTAATTTTTCGTTCTTTTTTTATTACAACAGATGTGGGGAGGAGGATTGAACATTTGATCTCAGTGAAAAGTAACTGGTGCTTTAGCTATGCTCGGATTCACGTTAATTTTTAATTTTTTTTCTGGTTTGTTATAGCAATAGATCATATGGTGGAAAGATCAATCTGTAATGCAAATCCCCAACCTGTTTGGAGGTACGTGCAGATCTATTTAATTGAAGCATCTCAATTGATAATTTTCTTTCATTTTCTCGGCAGCCAAACAAAGGATAAGCCTTAATTTTGGATGTTGTTGTCAGGCAACTGCCGTAAAATGGGTGCCAATGGTTGTAACTTTTGGTGTGGTGCTTTTGATTCTTTGGGCTTCCTTTGTTCTCTTTCACATCTCATTATCTCATCATAATGAATTTGTAATTCTATATAACAACAGAAAACTGATTCAC

mRNA sequence

ATTGTTGGAAGGCTTAGGGATGGGCTGCCACTTGCACAGGGCATTGGGTATTTTAATGAAGAGAATGATGTTTTAGGCTACAAACAGCAAGCTGAATTCATACTCAAACACATCTCTAGAGGGGCCTTCTCACCTTCAAAAATGGCCATTCTGATTCATCATCAATGTTTCATTTACTTGGTGGAGAATGGTGTCTGCTGCATCACATTGTGCGATTCTTCATACCCAAGAAAGCTGGCTCTCCATTACTTGCAAGATTTGCAAAAGGAGCTTGCCAAGTTTGATGATGCCCTAATAAACCTTACCAAACCCAATAGTTTTGCCAAATTTGATAGAATAATTAGCAATATTAGAAAACGGTACATTGATACAAGGACACAAGCCAATCTACATAAGCTCAATGCAGATCCTGGCAGGTGTCCAGACATTGTCACATGTTCACTCTTTGAAATTTTAGAAAGGAAAAAACTAGATCATATGGTGGAAAGATCAATCTGTAATGCAAATCCCCAACCTGTTTGGAGGATAAGCCTTAATTTTGGATGTTGTTGTCAGGCAACTGCCGTAAAATGGGTGCCAATGGTTGTAACTTTTGGTGTGGTGCTTTTGATTCTTTGGGCTTCCTTTGTTCTCTTTCACATCTCATTATCTCATCATAATGAATTTGTAATTCTATATAACAACAGAAAACTGATTCAC

Coding sequence (CDS)

ATTGTTGGAAGGCTTAGGGATGGGCTGCCACTTGCACAGGGCATTGGGTATTTTAATGAAGAGAATGATGTTTTAGGCTACAAACAGCAAGCTGAATTCATACTCAAACACATCTCTAGAGGGGCCTTCTCACCTTCAAAAATGGCCATTCTGATTCATCATCAATGTTTCATTTACTTGGTGGAGAATGGTGTCTGCTGCATCACATTGTGCGATTCTTCATACCCAAGAAAGCTGGCTCTCCATTACTTGCAAGATTTGCAAAAGGAGCTTGCCAAGTTTGATGATGCCCTAATAAACCTTACCAAACCCAATAGTTTTGCCAAATTTGATAGAATAATTAGCAATATTAGAAAACGGTACATTGATACAAGGACACAAGCCAATCTACATAAGCTCAATGCAGATCCTGGCAGGTGTCCAGACATTGTCACATGTTCACTCTTTGAAATTTTAGAAAGGAAAAAACTAGATCATATGGTGGAAAGATCAATCTGTAATGCAAATCCCCAACCTGTTTGGAGGATAAGCCTTAATTTTGGATGTTGTTGTCAGGCAACTGCCGTAAAATGGGTGCCAATGGTTGTAACTTTTGGTGTGGTGCTTTTGATTCTTTGGGCTTCCTTTGTTCTCTTTCACATCTCATTATCTCATCATAATGAATTTGTAATTCTATATAACAACAGAAAACTGATTCAC

Protein sequence

IVGRLRDGLPLAQGIGYFNEENDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIYLVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALINLTKPNSFAKFDRIISNIRKRYIDTRTQANLHKLNADPGRCPDIVTCSLFEILERKKLDHMVERSICNANPQPVWRISLNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH

Homology

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BLAST of MS000399 vs. NCBI nr
Match: XP_022141127.1 (LOW QUALITY PROTEIN: 25.3 kDa vesicle transport protein [Momordica charantia])

HSP 1 Score: 411.0 bits (1055), Expect = 6.9e-111
Identity = 213/236 (90.25%), Postives = 214/236 (90.68%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           IVGRLRDGLPLAQGIGYFNEEN DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY
Sbjct: 6   IVGRLRDGLPLAQGIGYFNEENDDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN LTKPNSFAKFDRIISNIR
Sbjct: 66  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALINXLTKPNSFAKFDRIISNIR 125

Query: 121 KRYIDTRTQANLHKLNADPG-RCPDIVTCSLFEILERKKLDHMVERSICNANPQPVWRIS 180
           KRYIDTRTQANLHKLNA+P  RCPDIVTCSLFEILERKKL                 RIS
Sbjct: 126 KRYIDTRTQANLHKLNANPXLRCPDIVTCSLFEILERKKLAKQ--------------RIS 185

Query: 181 LNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           LN GCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH
Sbjct: 186 LNVGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 227

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BLAST of MS000399 vs. NCBI nr
Match: XP_022968542.1 (25.3 kDa vesicle transport protein isoform X1 [Cucurbita maxima])

HSP 1 Score: 318.2 bits (814), Expect = 6.1e-83
Identity = 165/237 (69.62%), Postives = 188/237 (79.32%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDG+PLAQGI Y NEEN DVLGYKQQAEFILK ISRGA S  KMAI +HH  FIY
Sbjct: 6   MVGRVRDGVPLAQGIRYVNEENDDVLGYKQQAEFILKEISRGALSSPKMAIQMHHHSFIY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           L+ENGVC +TLCDS YPRKLA+HYLQDLQ+E +KFDDAL+N L KP SF KFD IISNIR
Sbjct: 66  LIENGVCYMTLCDSLYPRKLAIHYLQDLQREFSKFDDALLNSLVKPYSFPKFDNIISNIR 125

Query: 121 KRYIDTRTQANLHKLNADPGRCP-DIVTCSLFEILE-RKKLDHMVERSICNANPQPVWRI 180
           KRYIDTRTQ NL+KLN    +   DIVTCS+ EI E R+K+  MVERSICNAN   +W  
Sbjct: 126 KRYIDTRTQVNLYKLNDSRSQTDLDIVTCSISEIFERRRKITDMVERSICNANSSSIWSF 185

Query: 181 SLNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           S       +   +KW P+VVTFGVVL++LWASF+LF ISLSHHN+FVILYNNRKL H
Sbjct: 186 SF-----VKEIGLKWTPVVVTFGVVLVLLWASFLLFDISLSHHNQFVILYNNRKLFH 237

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BLAST of MS000399 vs. NCBI nr
Match: XP_022944814.1 (25.3 kDa vesicle transport protein isoform X1 [Cucurbita moschata])

HSP 1 Score: 317.4 bits (812), Expect = 1.0e-82
Identity = 163/237 (68.78%), Postives = 187/237 (78.90%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDG+PLAQGI Y NEEN DVLGYKQQAEFILK ISRGA SP KM I +HH  FIY
Sbjct: 49  MVGRVRDGVPLAQGIRYVNEENDDVLGYKQQAEFILKEISRGALSPPKMVIQMHHHSFIY 108

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           L+ENGVC +TLCDS YPRKLA+HYLQDLQ+E +KFDDAL+N L KP SF KFD II NIR
Sbjct: 109 LIENGVCYMTLCDSLYPRKLAIHYLQDLQREFSKFDDALLNSLVKPYSFLKFDNIIINIR 168

Query: 121 KRYIDTRTQANLHKLNADPGRCP-DIVTCSLFEILE-RKKLDHMVERSICNANPQPVWRI 180
           KRYIDTRTQ NL+KLN   G+   D+VTCS FEI E R+K+    ERSICNAN   +W  
Sbjct: 169 KRYIDTRTQVNLYKLNDGRGQTDLDMVTCSFFEIFERRRKITDRAERSICNANSSSIWSF 228

Query: 181 SLNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           S+      +   +KW P+VVTFGVVL++LWASF+LF ISLSHHN+FVILYNNRKL H
Sbjct: 229 SI-----VKEIGLKWTPVVVTFGVVLVLLWASFLLFDISLSHHNQFVILYNNRKLFH 280

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BLAST of MS000399 vs. NCBI nr
Match: XP_038875892.1 (25.3 kDa vesicle transport protein [Benincasa hispida])

HSP 1 Score: 316.6 bits (810), Expect = 1.8e-82
Identity = 167/236 (70.76%), Postives = 189/236 (80.08%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDGLP+AQGI Y NEEN DVLGYK+QAEFILK ISRG  S SKMAIL+HH CFIY
Sbjct: 6   MVGRVRDGLPIAQGIRYINEENDDVLGYKKQAEFILKEISRGTLSSSKMAILMHHHCFIY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           LVENGVC +TLCDSSYPRKLALHYLQDLQKE+ +F+ ALIN L KP S  +FD IISNIR
Sbjct: 66  LVENGVCYMTLCDSSYPRKLALHYLQDLQKEVGEFEYALINKLIKPYSLTQFDGIISNIR 125

Query: 121 KRYIDTRTQANLHKLNADPGRC-PDIVTCSLFEILERKKLDHMVERSICNANPQPVWRIS 180
           K+YIDTRTQANL KLNA+  +  PD++TCSL EILER++   MVERS+CN         S
Sbjct: 126 KQYIDTRTQANLCKLNANRRQADPDMLTCSLSEILERRRKLDMVERSMCNNTNSQGASSS 185

Query: 181 LNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           +    C Q   +KW PMVVTFGVVL+ILWASF+LF ISLS HNEFVILYNNRKL H
Sbjct: 186 IWGSPCLQEIGLKWTPMVVTFGVVLIILWASFLLFDISLSQHNEFVILYNNRKLFH 241

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BLAST of MS000399 vs. NCBI nr
Match: XP_023541413.1 (25.3 kDa vesicle transport protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 311.2 bits (796), Expect = 7.4e-81
Identity = 160/237 (67.51%), Postives = 188/237 (79.32%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEE-NDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDG+PLAQGI Y NEE +DVLGYKQQAEFILK ISRGA S  KMAI +HH  FIY
Sbjct: 18  MVGRVRDGVPLAQGIRYVNEETDDVLGYKQQAEFILKQISRGALSSPKMAIQMHHHSFIY 77

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           L+ENGVC +TLCDS YPRKLA+HYLQDLQ+E +KFDDAL+N L KP S+ KFD IISNIR
Sbjct: 78  LMENGVCYMTLCDSLYPRKLAIHYLQDLQREFSKFDDALLNSLVKPYSYPKFDNIISNIR 137

Query: 121 KRYIDTRTQANLHKLNADPGRCP-DIVTCSLFEILE-RKKLDHMVERSICNANPQPVWRI 180
           KRYIDTRTQANL+KLN + G+   D++TCS  EI E R+K+   +ERSICNAN   +W  
Sbjct: 138 KRYIDTRTQANLYKLNDNRGQIDLDMITCSFSEIFERRRKITDTMERSICNANSSSIWSY 197

Query: 181 SLNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           S       +   +KW PMVVTFG VL++LWASF+LF ISL+HHN+FVILYNNRKL H
Sbjct: 198 SF-----LKEIGLKWTPMVVTFGAVLVLLWASFLLFDISLAHHNQFVILYNNRKLFH 249

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BLAST of MS000399 vs. ExPASy Swiss-Prot
Match: Q94AU2 (25.3 kDa vesicle transport protein OS=Arabidopsis thaliana OX=3702 GN=SEC22 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 8.1e-30
Identity = 76/208 (36.54%), Postives = 115/208 (55.29%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEENDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIYL 60
           ++ R+ DGLPLA+G+    +  D   YKQQ + + K++SRG    S+M++      F Y+
Sbjct: 6   LIARVTDGLPLAEGLDDGRDLPDSDMYKQQVKALFKNLSRGQNDASRMSVETGPYVFHYI 65

Query: 61  VENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALI-NLTKPNSFAKFDRIISNIRK 120
           +E  VC +T+CD SYP+KLA  YL+DL+ E  + +   I    +P +F KFD  I   +K
Sbjct: 66  IEGRVCYLTMCDRSYPKKLAFQYLEDLKNEFERVNGPNIETAARPYAFIKFDTFIQKTKK 125

Query: 121 RYIDTRTQANLHKLNADPGRCPDIVTCSLFEIL-ERKKLDHMVERSICNANPQPVWRISL 180
            Y DTRTQ N+ KLN +      I+T ++ E+L   +KLD + E S    +   ++    
Sbjct: 126 LYQDTRTQRNIAKLNDELYEVHQIMTRNVQEVLGVGEKLDQVSEMSSRLTSESRIYADKA 185

Query: 181 NFGCCCQATAVKWVPMVVTFGVVLLILW 207
                 QA   KW P+ + FGVV L+ W
Sbjct: 186 K-DLNRQALIRKWAPVAIVFGVVFLLFW 212

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BLAST of MS000399 vs. ExPASy Swiss-Prot
Match: Q7SXP0 (Vesicle-trafficking protein SEC22b-B OS=Danio rerio OX=7955 GN=sec22bb PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.4e-18
Identity = 51/160 (31.87%), Postives = 91/160 (56.88%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEENDVLG-----YKQQAEFILKHISRGAFSPSKMAILIHHQ 60
           ++ RL DGLPLA  +    +E++ +G     Y+ QA+ + + ++    SP++  +     
Sbjct: 6   MIARLADGLPLAASM----QEDEQMGRDLQQYQSQAKQLFRKLNEQ--SPNRCTLEAGSM 65

Query: 61  CFIYLVENGVCCITLCDSSYPRKLALHYLQDLQKEL-AKFDDALINLTKPNSFAKFDRII 120
            F Y++E GVC + LC++ +P+KLA  YL+DLQ E   +    +  +++P SF +FD  I
Sbjct: 66  SFHYVIEKGVCYLVLCEAGFPKKLAFAYLEDLQAEFHEQHGKKVPTVSRPYSFIEFDTYI 125

Query: 121 SNIRKRYIDTRTQANLHKLNADPGRCPDIVTCSLFEILER 155
              +K YID+R + NL  +N +      I+  ++ E+L+R
Sbjct: 126 QKTKKSYIDSRARRNLSNINTELQDVQRIMVANIEEVLQR 159

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BLAST of MS000399 vs. ExPASy Swiss-Prot
Match: Q5ZJW4 (Vesicle-trafficking protein SEC22b OS=Gallus gallus OX=9031 GN=SEC22B PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.9e-18
Identity = 49/156 (31.41%), Postives = 89/156 (57.05%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEE-NDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           ++ R+ DGLPLA  +    +   D+  Y+ QA+ + + ++    SP++  +      F Y
Sbjct: 6   MIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQ--SPTRCTLEAGAMAFHY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKEL-AKFDDALINLTKPNSFAKFDRIISNIR 120
           ++E GVC + LC++++P+KLA  YL+DLQ E   +    +  +++P SF +FD  I   +
Sbjct: 66  IIEKGVCYLVLCEAAFPKKLAFAYLEDLQSEFDEQHGKKVPTVSRPYSFIEFDTYIQKTK 125

Query: 121 KRYIDTRTQANLHKLNADPGRCPDIVTCSLFEILER 155
           K YID+R + NL  +N +      I+  ++ E+L+R
Sbjct: 126 KLYIDSRARRNLGSINTELQDVQRIMVANIEEVLQR 159

[Close]

BLAST of MS000399 vs. ExPASy Swiss-Prot
Match: Q7ZV15 (Vesicle-trafficking protein SEC22b-A OS=Danio rerio OX=7955 GN=sec22ba PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.2e-18
Identity = 49/156 (31.41%), Postives = 91/156 (58.33%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEE-NDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           ++ R+ D LPLA  +    +   D+  Y+ QA+ + + ++    SP++  +     CF Y
Sbjct: 6   MIVRVADSLPLAASMQEDEQSGRDLQKYQSQAKQLCRKLNEQ--SPARCTLEAGKMCFHY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALI-NLTKPNSFAKFDRIISNIR 120
           ++E GVC + LC++ +P+KLA  YL+DL+ E ++   A + ++++P SF +FD  I    
Sbjct: 66  VIEKGVCYLALCEAGFPKKLAFAYLEDLEGEFSEQYGAKVPSVSRPYSFIEFDTYIQKTI 125

Query: 121 KRYIDTRTQANLHKLNADPGRCPDIVTCSLFEILER 155
           K YID+R + NL  +N++      I+  ++ E+L+R
Sbjct: 126 KSYIDSRARRNLGNINSELHDVQRIMVANIEEVLQR 159

[Close]

BLAST of MS000399 vs. ExPASy Swiss-Prot
Match: O08547 (Vesicle-trafficking protein SEC22b OS=Mus musculus OX=10090 GN=Sec22b PE=1 SV=3)

HSP 1 Score: 92.4 bits (228), Expect = 7.1e-18
Identity = 48/156 (30.77%), Postives = 88/156 (56.41%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEE-NDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           ++ R+ DGLPLA  +    +   D+  Y+ QA+ + + ++    SP++  +      F Y
Sbjct: 6   MIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQ--SPTRCTLEAGAMTFHY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKEL-AKFDDALINLTKPNSFAKFDRIISNIR 120
           ++E GVC + LC++++P+KLA  YL+DL  E   +    +  +++P SF +FD  I   +
Sbjct: 66  IIEQGVCYLVLCEAAFPKKLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTK 125

Query: 121 KRYIDTRTQANLHKLNADPGRCPDIVTCSLFEILER 155
           K YID+R + NL  +N +      I+  ++ E+L+R
Sbjct: 126 KLYIDSRARRNLGSINTELQDVQRIMVANIEEVLQR 159

[Close]

BLAST of MS000399 vs. ExPASy TrEMBL
Match: A0A6J1CIZ8 (LOW QUALITY PROTEIN: 25.3 kDa vesicle transport protein OS=Momordica charantia OX=3673 GN=LOC111011599 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.3e-111
Identity = 213/236 (90.25%), Postives = 214/236 (90.68%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           IVGRLRDGLPLAQGIGYFNEEN DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY
Sbjct: 6   IVGRLRDGLPLAQGIGYFNEENDDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN LTKPNSFAKFDRIISNIR
Sbjct: 66  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALINXLTKPNSFAKFDRIISNIR 125

Query: 121 KRYIDTRTQANLHKLNADPG-RCPDIVTCSLFEILERKKLDHMVERSICNANPQPVWRIS 180
           KRYIDTRTQANLHKLNA+P  RCPDIVTCSLFEILERKKL                 RIS
Sbjct: 126 KRYIDTRTQANLHKLNANPXLRCPDIVTCSLFEILERKKLAKQ--------------RIS 185

Query: 181 LNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           LN GCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH
Sbjct: 186 LNVGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 227

[Close]

BLAST of MS000399 vs. ExPASy TrEMBL
Match: A0A6J1HTS6 (25.3 kDa vesicle transport protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467744 PE=3 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.9e-83
Identity = 165/237 (69.62%), Postives = 188/237 (79.32%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDG+PLAQGI Y NEEN DVLGYKQQAEFILK ISRGA S  KMAI +HH  FIY
Sbjct: 6   MVGRVRDGVPLAQGIRYVNEENDDVLGYKQQAEFILKEISRGALSSPKMAIQMHHHSFIY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           L+ENGVC +TLCDS YPRKLA+HYLQDLQ+E +KFDDAL+N L KP SF KFD IISNIR
Sbjct: 66  LIENGVCYMTLCDSLYPRKLAIHYLQDLQREFSKFDDALLNSLVKPYSFPKFDNIISNIR 125

Query: 121 KRYIDTRTQANLHKLNADPGRCP-DIVTCSLFEILE-RKKLDHMVERSICNANPQPVWRI 180
           KRYIDTRTQ NL+KLN    +   DIVTCS+ EI E R+K+  MVERSICNAN   +W  
Sbjct: 126 KRYIDTRTQVNLYKLNDSRSQTDLDIVTCSISEIFERRRKITDMVERSICNANSSSIWSF 185

Query: 181 SLNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           S       +   +KW P+VVTFGVVL++LWASF+LF ISLSHHN+FVILYNNRKL H
Sbjct: 186 SF-----VKEIGLKWTPVVVTFGVVLVLLWASFLLFDISLSHHNQFVILYNNRKLFH 237

[Close]

BLAST of MS000399 vs. ExPASy TrEMBL
Match: A0A6J1FZ74 (25.3 kDa vesicle transport protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449240 PE=3 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 5.0e-83
Identity = 163/237 (68.78%), Postives = 187/237 (78.90%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDG+PLAQGI Y NEEN DVLGYKQQAEFILK ISRGA SP KM I +HH  FIY
Sbjct: 49  MVGRVRDGVPLAQGIRYVNEENDDVLGYKQQAEFILKEISRGALSPPKMVIQMHHHSFIY 108

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           L+ENGVC +TLCDS YPRKLA+HYLQDLQ+E +KFDDAL+N L KP SF KFD II NIR
Sbjct: 109 LIENGVCYMTLCDSLYPRKLAIHYLQDLQREFSKFDDALLNSLVKPYSFLKFDNIIINIR 168

Query: 121 KRYIDTRTQANLHKLNADPGRCP-DIVTCSLFEILE-RKKLDHMVERSICNANPQPVWRI 180
           KRYIDTRTQ NL+KLN   G+   D+VTCS FEI E R+K+    ERSICNAN   +W  
Sbjct: 169 KRYIDTRTQVNLYKLNDGRGQTDLDMVTCSFFEIFERRRKITDRAERSICNANSSSIWSF 228

Query: 181 SLNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKLIH 234
           S+      +   +KW P+VVTFGVVL++LWASF+LF ISLSHHN+FVILYNNRKL H
Sbjct: 229 SI-----VKEIGLKWTPVVVTFGVVLVLLWASFLLFDISLSHHNQFVILYNNRKLFH 280

[Close]

BLAST of MS000399 vs. ExPASy TrEMBL
Match: A0A0A0LBE2 (Longin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G182070 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.4e-80
Identity = 165/235 (70.21%), Postives = 184/235 (78.30%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDGLP+AQGI Y NEEN DV GYK+QAEFILK ISRG  SP KM IL+HH  FIY
Sbjct: 6   MVGRVRDGLPIAQGIRYVNEENDDVFGYKKQAEFILKEISRGTLSPPKMVILMHHHSFIY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           LVENGVC +TLCDSSYPRKLA+HYLQDLQKE  KFDDALIN L KP SF KFD IISN+R
Sbjct: 66  LVENGVCYMTLCDSSYPRKLAIHYLQDLQKEFGKFDDALINKLIKPYSFLKFDSIISNVR 125

Query: 121 KRYIDTRTQANLHKLNADPGR-CPDIVTCSLFEILERKKLDHMVERSICNANPQPVWRIS 180
           K+YIDTRTQANL KLNA+  +  PDI TCSL EILER++   MVERS  N N        
Sbjct: 126 KQYIDTRTQANLSKLNANRRQPDPDISTCSLSEILERRRKFDMVERSRSNNNTNSEGNSC 185

Query: 181 LNFGC-CCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKL 232
             +G    +   VKW PMVVTFGVVL++LWASF+LF ISLSHHN+FVILYNNRKL
Sbjct: 186 SIWGSPYLEEIGVKWTPMVVTFGVVLIVLWASFLLFDISLSHHNQFVILYNNRKL 240

[Close]

BLAST of MS000399 vs. ExPASy TrEMBL
Match: A0A1S3AYX3 (25.3 kDa vesicle transport protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484073 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.2e-79
Identity = 162/234 (69.23%), Postives = 184/234 (78.63%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEEN-DVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIY 60
           +VGR+RDGLP+AQGI Y NEEN DV GYK+QAEFILK ISRG  SP KM IL+HH  FIY
Sbjct: 6   MVGRVRDGLPIAQGIRYVNEENDDVFGYKKQAEFILKEISRGTLSPPKMVILMHHHSFIY 65

Query: 61  LVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALIN-LTKPNSFAKFDRIISNIR 120
           LVENGVC +TLCDSSYPRKLALHYLQDLQKE  KFDDALIN L KP SF KFD IISN+R
Sbjct: 66  LVENGVCYMTLCDSSYPRKLALHYLQDLQKEFGKFDDALINKLIKPYSFLKFDSIISNVR 125

Query: 121 KRYIDTRTQANLHKLNADPGR-CPDIVTCSLFEILERKKLDHMVERSICNANPQPVWRIS 180
           K+YIDTRTQ NL KLNA+  +  PDI++CS  EILER++   + ERS CN N Q     S
Sbjct: 126 KQYIDTRTQVNLCKLNANRRQPDPDILSCSFSEILERRRKLDIEERSRCNTNSQGT-SSS 185

Query: 181 LNFGCCCQATAVKWVPMVVTFGVVLLILWASFVLFHISLSHHNEFVILYNNRKL 232
           +      +   +KW PMVVTFGVVL++LWASF+LF ISLSHHN+FVILYNNRKL
Sbjct: 186 IWGSPYLEEIGLKWTPMVVTFGVVLIVLWASFLLFDISLSHHNQFVILYNNRKL 238

[Close]

BLAST of MS000399 vs. TAIR 10
Match: AT5G52270.1 (SNARE-like superfamily protein )

HSP 1 Score: 157.9 bits (398), Expect = 9.8e-39
Identity = 87/161 (54.04%), Postives = 114/161 (70.81%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEENDV--LGYKQQAEFILKHISRGAFSPSKMAILIHHQCFI 60
           IVGR+ DGLPLAQ   Y N+E++   L YKQQAEF+LK +S+ +    KM IL+ H  F 
Sbjct: 6   IVGRVEDGLPLAQDQTYVNQEDNTSFLLYKQQAEFLLKQVSKDSLLHPKMTILLDHHSFH 65

Query: 61  YLVENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDD-ALI-NLTKPNSFAKFDRIISN 120
           +LVE  +C I L DSSYPRKL  +YLQ+L KEL K D+ ALI  ++KP SF +F +II  
Sbjct: 66  FLVEKKICYIALSDSSYPRKLLFNYLQNLNKELDKLDEKALIQKISKPYSFIRFGKIIGR 125

Query: 121 IRKRYIDTRTQANLHKLNADPGRCPDIVTCSLFEILERKKL 158
           IRK+YIDTRTQANL KLNA   +  D+VT  L +I++R+++
Sbjct: 126 IRKQYIDTRTQANLSKLNALRKQELDVVTEHLNDIIQRQQI 166

[Close]

BLAST of MS000399 vs. TAIR 10
Match: AT1G11890.1 (Synaptobrevin family protein )

HSP 1 Score: 132.1 bits (331), Expect = 5.7e-31
Identity = 76/208 (36.54%), Postives = 115/208 (55.29%), Query Frame = 0


Query: 1   IVGRLRDGLPLAQGIGYFNEENDVLGYKQQAEFILKHISRGAFSPSKMAILIHHQCFIYL 60
           ++ R+ DGLPLA+G+    +  D   YKQQ + + K++SRG    S+M++      F Y+
Sbjct: 6   LIARVTDGLPLAEGLDDGRDLPDSDMYKQQVKALFKNLSRGQNDASRMSVETGPYVFHYI 65

Query: 61  VENGVCCITLCDSSYPRKLALHYLQDLQKELAKFDDALI-NLTKPNSFAKFDRIISNIRK 120
           +E  VC +T+CD SYP+KLA  YL+DL+ E  + +   I    +P +F KFD  I   +K
Sbjct: 66  IEGRVCYLTMCDRSYPKKLAFQYLEDLKNEFERVNGPNIETAARPYAFIKFDTFIQKTKK 125

Query: 121 RYIDTRTQANLHKLNADPGRCPDIVTCSLFEIL-ERKKLDHMVERSICNANPQPVWRISL 180
            Y DTRTQ N+ KLN +      I+T ++ E+L   +KLD + E S    +   ++    
Sbjct: 126 LYQDTRTQRNIAKLNDELYEVHQIMTRNVQEVLGVGEKLDQVSEMSSRLTSESRIYADKA 185

Query: 181 NFGCCCQATAVKWVPMVVTFGVVLLILW 207
                 QA   KW P+ + FGVV L+ W
Sbjct: 186 K-DLNRQALIRKWAPVAIVFGVVFLLFW 212

The following BLAST results are available for this feature:

BLAST of MS000399 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141127.1	6.9e-111	90.25	LOW QUALITY PROTEIN: 25.3 kDa vesicle transport protein [Momordica charantia]	[more]
XP_022968542.1	6.1e-83	69.62	25.3 kDa vesicle transport protein isoform X1 [Cucurbita maxima]	[more]
XP_022944814.1	1.0e-82	68.78	25.3 kDa vesicle transport protein isoform X1 [Cucurbita moschata]	[more]
XP_038875892.1	1.8e-82	70.76	25.3 kDa vesicle transport protein [Benincasa hispida]	[more]
XP_023541413.1	7.4e-81	67.51	25.3 kDa vesicle transport protein [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS000399 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AU2	8.1e-30	36.54	25.3 kDa vesicle transport protein OS=Arabidopsis thaliana OX=3702 GN=SEC22 PE=2...	[more]
Q7SXP0	1.4e-18	31.88	Vesicle-trafficking protein SEC22b-B OS=Danio rerio OX=7955 GN=sec22bb PE=2 SV=1	[more]
Q5ZJW4	1.9e-18	31.41	Vesicle-trafficking protein SEC22b OS=Gallus gallus OX=9031 GN=SEC22B PE=2 SV=1	[more]
Q7ZV15	4.2e-18	31.41	Vesicle-trafficking protein SEC22b-A OS=Danio rerio OX=7955 GN=sec22ba PE=2 SV=1	[more]
O08547	7.1e-18	30.77	Vesicle-trafficking protein SEC22b OS=Mus musculus OX=10090 GN=Sec22b PE=1 SV=3	[more]

BLAST of MS000399 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIZ8	3.3e-111	90.25	LOW QUALITY PROTEIN: 25.3 kDa vesicle transport protein OS=Momordica charantia O...	[more]
A0A6J1HTS6	2.9e-83	69.62	25.3 kDa vesicle transport protein isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1FZ74	5.0e-83	68.78	25.3 kDa vesicle transport protein isoform X1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A0A0LBE2	1.4e-80	70.21	Longin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G182070 PE=3...	[more]
A0A1S3AYX3	1.2e-79	69.23	25.3 kDa vesicle transport protein isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034...	[more]

BLAST of MS000399 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52270.1	9.8e-39	54.04	SNARE-like superfamily protein	[more]
AT1G11890.1	5.7e-31	36.54	Synaptobrevin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010908	Longin domain	SMART	SM01270	Longin_2	coord: 30..113 e-value: 2.2E-15 score: 67.1
IPR010908	Longin domain	PFAM	PF13774	Longin	coord: 31..111 e-value: 1.2E-13 score: 50.9
IPR010908	Longin domain	PROSITE	PS50859	LONGIN	coord: 1..114 score: 17.654337
IPR010908	Longin domain	CDD	cd14824	Longin	coord: 2..121 e-value: 4.8483E-20 score: 80.3777
None	No IPR available	GENE3D	3.30.450.50	Longin domain	coord: 1..122 e-value: 1.6E-28 score: 101.1
None	No IPR available	PANTHER	PTHR45837:SF7	SNARE-LIKE SUPERFAMILY PROTEIN	coord: 1..212
IPR044565	Vesicle-trafficking protein Sec22	PANTHER	PTHR45837	VESICLE-TRAFFICKING PROTEIN SEC22B	coord: 1..212
IPR011012	Longin-like domain superfamily	SUPERFAMILY	64356	SNARE-like	coord: 2..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000399.1	MS000399.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport
biological_process	GO:0006890	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
molecular_function	GO:0005484	SNAP receptor activity