MS000394 (gene) Bitter gourd (TR) v1

Overview


Name	MS000394
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	3-hexulose-6-phosphate isomerase
Location	scaffold44: 1414806 .. 1415417 (-)
RNA-Seq Expression	MS000394
Synteny	MS000394

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCCAATCGCAGCCTTCTCTGGCCTCCAAAATATGCTCCGACATAGCTTCTATCTTCTCCAAAACCACGCACCCCCACGCGCCGCCCATGGACGTATTGGTATCCGAGCTTGCAGCCGCCGCGACCCGAAAGGCCCGGGTCTTCCTCTACGGCGTCGGCCGGGAGGGCCTAATGCTCAAAGCCCTATGCATGCGACTCGCTCATCTCGGCCTCTCATCGCACCAGGTTTTCGACATGAACGCGCCGCCAATCGCGGCTCAGGACCTCCTCATCGCATCCGCCGGCCCAGGCGGATTTTCGACCGTCGATGCGATTTGTTCCGTGGCGAAATCTAACGGCGGTAGGGTTCTGCTAGTGACGGCCCAGCCGGAGACCGGGTCGTCGGTGAAGCACGCGGATGTGATTTGTTACGTTCCGGCGCAGACCATGGCGGACGATGAGGAAGCGGCGGCGGCGGAGGGAGTGAAATCTCGGCGGCCTCTGCTTCCGATGGGAAGTTTGTATGAAGGGGCTCTGTTCGTGTTGTTTGAGATGGTGGTGTATCGGCTGGGTGAGGCGTTGGGAGAGACGCCAGAAGCCGTCCGATCACGCCACACCAATCTAGAA

mRNA sequence

ATGGCTTCCCAATCGCAGCCTTCTCTGGCCTCCAAAATATGCTCCGACATAGCTTCTATCTTCTCCAAAACCACGCACCCCCACGCGCCGCCCATGGACGTATTGGTATCCGAGCTTGCAGCCGCCGCGACCCGAAAGGCCCGGGTCTTCCTCTACGGCGTCGGCCGGGAGGGCCTAATGCTCAAAGCCCTATGCATGCGACTCGCTCATCTCGGCCTCTCATCGCACCAGGTTTTCGACATGAACGCGCCGCCAATCGCGGCTCAGGACCTCCTCATCGCATCCGCCGGCCCAGGCGGATTTTCGACCGTCGATGCGATTTGTTCCGTGGCGAAATCTAACGGCGGTAGGGTTCTGCTAGTGACGGCCCAGCCGGAGACCGGGTCGTCGGTGAAGCACGCGGATGTGATTTGTTACGTTCCGGCGCAGACCATGGCGGACGATGAGGAAGCGGCGGCGGCGGAGGGAGTGAAATCTCGGCGGCCTCTGCTTCCGATGGGAAGTTTGTATGAAGGGGCTCTGTTCGTGTTGTTTGAGATGGTGGTGTATCGGCTGGGTGAGGCGTTGGGAGAGACGCCAGAAGCCGTCCGATCACGCCACACCAATCTAGAA

Coding sequence (CDS)

ATGGCTTCCCAATCGCAGCCTTCTCTGGCCTCCAAAATATGCTCCGACATAGCTTCTATCTTCTCCAAAACCACGCACCCCCACGCGCCGCCCATGGACGTATTGGTATCCGAGCTTGCAGCCGCCGCGACCCGAAAGGCCCGGGTCTTCCTCTACGGCGTCGGCCGGGAGGGCCTAATGCTCAAAGCCCTATGCATGCGACTCGCTCATCTCGGCCTCTCATCGCACCAGGTTTTCGACATGAACGCGCCGCCAATCGCGGCTCAGGACCTCCTCATCGCATCCGCCGGCCCAGGCGGATTTTCGACCGTCGATGCGATTTGTTCCGTGGCGAAATCTAACGGCGGTAGGGTTCTGCTAGTGACGGCCCAGCCGGAGACCGGGTCGTCGGTGAAGCACGCGGATGTGATTTGTTACGTTCCGGCGCAGACCATGGCGGACGATGAGGAAGCGGCGGCGGCGGAGGGAGTGAAATCTCGGCGGCCTCTGCTTCCGATGGGAAGTTTGTATGAAGGGGCTCTGTTCGTGTTGTTTGAGATGGTGGTGTATCGGCTGGGTGAGGCGTTGGGAGAGACGCCAGAAGCCGTCCGATCACGCCACACCAATCTAGAA

Protein sequence

MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLMLKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLLVTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEMVVYRLGEALGETPEAVRSRHTNLE

Homology

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BLAST of MS000394 vs. NCBI nr
Match: XP_022141151.1 (uncharacterized protein LOC111011614 [Momordica charantia])

HSP 1 Score: 393.3 bits (1009), Expect = 1.3e-105
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM
Sbjct: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM
Sbjct: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVYRLGEALGETPEAVRSRHTNLE
Sbjct: 181 VVYRLGEALGETPEAVRSRHTNLE 204

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BLAST of MS000394 vs. NCBI nr
Match: XP_022945534.1 (uncharacterized protein LOC111449742 [Cucurbita moschata] >XP_023542323.1 uncharacterized protein LOC111802255 [Cucurbita pepo subsp. pepo] >KAG6574169.1 hypothetical protein SDJN03_28056, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013223.1 hxlB, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 342.0 bits (876), Expect = 3.4e-90
Identity = 177/204 (86.76%), Postives = 190/204 (93.14%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQS PSLAS+ICS+IAS+FSK T  H+PP+++LVSEL+AAA RKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFSKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIAA DLLIASAGPGGFSTVDAICSVA+SNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAHDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           +TAQPETGSSVKHADVICYVPAQTMADDEE+     VK  RPLLPMGSLYEGALFVLFEM
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKC-RPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVY+L EALGE+PEAVRSRHTNLE
Sbjct: 181 VVYQLAEALGESPEAVRSRHTNLE 203

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BLAST of MS000394 vs. NCBI nr
Match: XP_022968332.1 (uncharacterized protein LOC111467597 [Cucurbita maxima])

HSP 1 Score: 340.1 bits (871), Expect = 1.3e-89
Identity = 176/204 (86.27%), Postives = 190/204 (93.14%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQS PSLAS+ICS+IAS+F K T  H+PP+++LVSEL+AAA RKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFFKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIAA+DLLIASAGPGGFSTVDAICSVA+SNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAARDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           +TAQPETGSSVKHADVICYVPAQTMADDEE+     VK  RPLLPMGSLYEGALFVLFEM
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKC-RPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVY+L EALGE+PEAVRSRHTNLE
Sbjct: 181 VVYQLAEALGESPEAVRSRHTNLE 203

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BLAST of MS000394 vs. NCBI nr
Match: XP_038901080.1 (3-hexulose-6-phosphate isomerase [Benincasa hispida])

HSP 1 Score: 339.3 bits (869), Expect = 2.2e-89
Identity = 180/205 (87.80%), Postives = 190/205 (92.68%), Query Frame = 0


Query: 1   MASQSQ-PSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGL 60
           MASQSQ  SLAS+ICSDIASIFSK T PHAPP+D+LVSEL++AA RKARVFLYGVGREGL
Sbjct: 1   MASQSQASSLASRICSDIASIFSKPTQPHAPPLDLLVSELSSAAARKARVFLYGVGREGL 60

Query: 61  MLKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVL 120
           MLKALCMRLAHLGLSSHQVFDMNAPPIAA DLLIASAGPGGFSTVDAICSVA+SNGGRVL
Sbjct: 61  MLKALCMRLAHLGLSSHQVFDMNAPPIAAPDLLIASAGPGGFSTVDAICSVARSNGGRVL 120

Query: 121 LVTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFE 180
           L+TAQPE GSSVK+ADVICYVPAQTMADDEE+AA       RPLLPMGSLYEGALFVLFE
Sbjct: 121 LLTAQPENGSSVKYADVICYVPAQTMADDEESAA-----KCRPLLPMGSLYEGALFVLFE 180

Query: 181 MVVYRLGEALGETPEAVRSRHTNLE 205
           MVVYRLGE LGE+PEAVRSRHTNLE
Sbjct: 181 MVVYRLGEVLGESPEAVRSRHTNLE 200

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BLAST of MS000394 vs. NCBI nr
Match: KAA0033631.1 (3-hexulose-6-phosphate isomerase [Cucumis melo var. makuwa] >TYK22930.1 3-hexulose-6-phosphate isomerase [Cucumis melo var. makuwa])

HSP 1 Score: 334.0 bits (855), Expect = 9.4e-88
Identity = 174/204 (85.29%), Postives = 187/204 (91.67%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQS  SLAS+ICS+IASIFSK T P+AP +D+LVSEL++ A RKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPSLDILVSELSSVAARKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVA+SNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           +TAQPE GSSVK+ADVICYVPAQTMADDEE+AAA      RPLLPMGSLYEGALFVLFEM
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESAAA---AECRPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVYR+GE LGE+PEAVRSRHTNLE
Sbjct: 181 VVYRIGEVLGESPEAVRSRHTNLE 201

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BLAST of MS000394 vs. ExPASy Swiss-Prot
Match: Q9YAK0 (Uncharacterized protein APE_1940.1 OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=APE_1940.1 PE=3 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 6.9e-17
Identity = 57/176 (32.39%), Postives = 88/176 (50.00%), Query Frame = 0


Query: 32  MDVLVSELAAAATRKARVFLYGVGREGLMLKALCMRLAHLGLSSHQVFDMNAPPIAAQDL 91
           +D  V EL      K +V + G GR GL+ KA  MRL HLG +S+ + +   P +   DL
Sbjct: 39  VDRFVGELERVYREKRKVLVMGAGRSGLVGKAFAMRLLHLGFNSYVLGETIVPSVREGDL 98

Query: 92  LIASAGPGGFSTVDAICSVAKSNGGRVLLVTAQPETGSSVKHADVICYVPAQTMA---DD 151
           ++A +G G    +      AK  G  V  +T  P++    + +D++  VP +T +   DD
Sbjct: 99  VVAISGSGRTKVIVTAAETAKQVGATVAAITTYPDSPLG-RLSDIVVRVPGRTKSSKMDD 158

Query: 152 EEAAAAEGVKSRRPLLPMGSLYEGALFVLFEMVVYRLGEALGETPEAVRSRHTNLE 205
             A    G+    PL P+G+L+E    V  + V+Y L   LG   E +R+ H N+E
Sbjct: 159 YFARQILGI--HEPLAPLGTLFEDTTMVFLDGVIYSLMTRLGIDEEYMRNMHANVE 211

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BLAST of MS000394 vs. ExPASy Swiss-Prot
Match: P42404 (3-hexulose-6-phosphate isomerase OS=Bacillus subtilis (strain 168) OX=224308 GN=hxlB PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.3e-15
Identity = 59/188 (31.38%), Postives = 95/188 (50.53%), Query Frame = 0


Query: 17  IASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLMLKALCMRLAHLGLSSH 76
           +A I ++  +  A   +    +LA       ++F  G GR GLM K+  MRL H+G ++H
Sbjct: 7   VAEILNELHNSAAYISNEEADQLADHILSSHQIFTAGAGRSGLMAKSFAMRLMHMGFNAH 66

Query: 77  QVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLLVTAQPETGSSVKHADV 136
            V ++  PP+A  DL+I  +G G   ++    + AKS  G V  +T  PE+ S  K AD+
Sbjct: 67  IVGEILTPPLAEGDLVIIGSGSGETKSLIHTAAKAKSLHGIVAALTINPES-SIGKQADL 126

Query: 137 ICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEMVVYRLGEALGETPEAV 196
           I  +P         +   +   S + + PMGSL+E  L + ++ V+ +L E  G   E +
Sbjct: 127 IIRMPG--------SPKDQSNGSYKTIQPMGSLFEQTLLLFYDAVILKLMEKKGLDSETM 185

Query: 197 RSRHTNLE 205
            + H NLE
Sbjct: 187 FTHHANLE 185

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BLAST of MS000394 vs. ExPASy Swiss-Prot
Match: O26351 (Uncharacterized protein MTH_249 OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=MTH_249 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-14
Identity = 52/173 (30.06%), Postives = 91/173 (52.60%), Query Frame = 0


Query: 32  MDVLVSELAAAATRKARVFLYGVGREGLMLKALCMRLAHLGLSSHQVFDMNAPPIAAQDL 91
           +++++S++  A++    VF+ G GR  L+ KA  MRL HLG   H V D+  P I  +D 
Sbjct: 30  LEMMISKIMDASS----VFIVGTGRSELIGKAFAMRLMHLGFKVHVVGDVTTPAIRDEDC 89

Query: 92  LIASAGPGGFSTVDAICSVAKSNGGRVLLVTAQPETGSSVKHADVICYVPAQTMADDEEA 151
           LIA +G G   TV      ++S G  V+ VTA PE+ +   ++DV+  +P++T    +  
Sbjct: 90  LIAISGSGETKTVTLAAETSRSVGATVVAVTATPES-TLTGYSDVVICIPSKTKEPWKYY 149

Query: 152 AAAEGVKSRRPLLPMGSLYEGALFVLFEMVVYRLGEALGETPEAVRSRHTNLE 205
            +         L PMG+L+E +  +  + ++      LG+  + ++ RH  +E
Sbjct: 150 TSGVLRGEYDDLTPMGTLFEDSTHLFLDGLIAEFMSILGKREKDLKERHAIIE 197

[Close]

BLAST of MS000394 vs. ExPASy Swiss-Prot
Match: Q9LBW5 (3-hexulose-6-phosphate isomerase OS=Mycobacterium gastri OX=1777 GN=rmpB PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 57/169 (33.73%), Postives = 79/169 (46.75%), Query Frame = 0


Query: 36  VSELAAAATRKARVFLYGVGREGLMLKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIAS 95
           V+ LA    +  RVF+ G GR GL+L+   MRL H GL+ H   D   P I+A DLL+ +
Sbjct: 41  VAVLARQIVQPGRVFVAGAGRSGLVLRMAAMRLMHFGLTVHVAGDTTTPAISAGDLLLVA 100

Query: 96  AGPGGFSTVDAICSVAKSNGGRVLLVTAQPETGSSVKHADVICYVPAQTMADDEEAAAAE 155
           +G G  S V      AK  G R+   T  P++      AD +  +PA    D     + +
Sbjct: 101 SGSGTTSGVVKSAETAKKAGARIAAFTTNPDS-PLAGLADAVVIIPAAQKTDHGSHISRQ 160

Query: 156 GVKSRRPLLPMGSLYEGALFVLFEMVVYRLGEALGETPEAVRSRHTNLE 205
                      GSL+E  LFV+ E V   L +      E + +RH NLE
Sbjct: 161 YA---------GSLFEQVLFVVTEAVFQSLWDHTEVEAEELWTRHANLE 199

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BLAST of MS000394 vs. ExPASy Swiss-Prot
Match: O28478 (Uncharacterized protein AF_1796 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1796 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.6e-10
Identity = 48/169 (28.40%), Postives = 82/169 (48.52%), Query Frame = 0


Query: 36  VSELAAAATRKARVFLYGVGREGLMLKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIAS 95
           V E+         +F+ G GR G + KA  MRL HLG + + V +   P I  QD+L+A 
Sbjct: 24  VGEMIKLIDSARSIFVIGAGRSGYIAKAFAMRLMHLGYTVYVVGETVTPRITDQDVLVAI 83

Query: 96  AGPGGFSTVDAICSVAKSNGGRVLLVTAQPETGSSVKHADVICYVPAQTMADDEEAAAAE 155
           +G G  ++V  I   AK  G +++ VT + ++ S  K ADV+  V  +   + +E  +  
Sbjct: 84  SGSGETTSVVNISKKAKDIGSKLVAVTGKRDS-SLAKMADVVMVVKGKMKQERDEILS-- 143

Query: 156 GVKSRRPLLPMGSLYEGALFVLFEMVVYRLGEALGETPEAVRSRHTNLE 205
                  L P+G+++E    +  + +V  +      T + + +RH  LE
Sbjct: 144 ------QLAPLGTMFELTAMIFLDALVAEIMMQKHLTEKDLEARHAVLE 183

[Close]

BLAST of MS000394 vs. ExPASy TrEMBL
Match: A0A6J1CI67 (uncharacterized protein LOC111011614 OS=Momordica charantia OX=3673 GN=LOC111011614 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.3e-106
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM
Sbjct: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM
Sbjct: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVYRLGEALGETPEAVRSRHTNLE
Sbjct: 181 VVYRLGEALGETPEAVRSRHTNLE 204

[Close]

BLAST of MS000394 vs. ExPASy TrEMBL
Match: A0A6J1G183 (uncharacterized protein LOC111449742 OS=Cucurbita moschata OX=3662 GN=LOC111449742 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.7e-90
Identity = 177/204 (86.76%), Postives = 190/204 (93.14%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQS PSLAS+ICS+IAS+FSK T  H+PP+++LVSEL+AAA RKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFSKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIAA DLLIASAGPGGFSTVDAICSVA+SNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAHDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           +TAQPETGSSVKHADVICYVPAQTMADDEE+     VK  RPLLPMGSLYEGALFVLFEM
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKC-RPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVY+L EALGE+PEAVRSRHTNLE
Sbjct: 181 VVYQLAEALGESPEAVRSRHTNLE 203

[Close]

BLAST of MS000394 vs. ExPASy TrEMBL
Match: A0A6J1HTB3 (uncharacterized protein LOC111467597 OS=Cucurbita maxima OX=3661 GN=LOC111467597 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.3e-90
Identity = 176/204 (86.27%), Postives = 190/204 (93.14%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQS PSLAS+ICS+IAS+F K T  H+PP+++LVSEL+AAA RKARVFLYGVGREGLM
Sbjct: 1   MASQSPPSLASRICSEIASVFFKPTRQHSPPLELLVSELSAAAARKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIAA+DLLIASAGPGGFSTVDAICSVA+SNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAARDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           +TAQPETGSSVKHADVICYVPAQTMADDEE+     VK  RPLLPMGSLYEGALFVLFEM
Sbjct: 121 LTAQPETGSSVKHADVICYVPAQTMADDEESMTETAVKC-RPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVY+L EALGE+PEAVRSRHTNLE
Sbjct: 181 VVYQLAEALGESPEAVRSRHTNLE 203

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BLAST of MS000394 vs. ExPASy TrEMBL
Match: A0A5A7SW26 (3-hexulose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00080 PE=4 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.5e-88
Identity = 174/204 (85.29%), Postives = 187/204 (91.67%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQS  SLAS+ICS+IASIFSK T P+AP +D+LVSEL++ A RKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPSLDILVSELSSVAARKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVA+SNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           +TAQPE GSSVK+ADVICYVPAQTMADDEE+AAA      RPLLPMGSLYEGALFVLFEM
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESAAA---AECRPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVYR+GE LGE+PEAVRSRHTNLE
Sbjct: 181 VVYRIGEVLGESPEAVRSRHTNLE 201

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BLAST of MS000394 vs. ExPASy TrEMBL
Match: A0A1S3AXT7 (3-hexulose-6-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103484066 PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 2.9e-87
Identity = 173/204 (84.80%), Postives = 186/204 (91.18%), Query Frame = 0


Query: 1   MASQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRKARVFLYGVGREGLM 60
           MASQS  SLAS+ICS+IASIFSK T P+AP +D+LVSEL++ A RKARVFLYGVGREGLM
Sbjct: 1   MASQSPASLASRICSEIASIFSKPTQPYAPSLDILVSELSSVAARKARVFLYGVGREGLM 60

Query: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 120
           LKALCMRLAHLGLSSHQVFDMNAPPIA  DLLIASAGPGGFSTVDAICSVA+SNGGRVLL
Sbjct: 61  LKALCMRLAHLGLSSHQVFDMNAPPIAGSDLLIASAGPGGFSTVDAICSVARSNGGRVLL 120

Query: 121 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 180
           +TAQPE GSSVK+ADVICYVPAQTMADDEE+AAA      RPLLPMGSLYEGALFVLFEM
Sbjct: 121 LTAQPENGSSVKYADVICYVPAQTMADDEESAAA---AECRPLLPMGSLYEGALFVLFEM 180

Query: 181 VVYRLGEALGETPEAVRSRHTNLE 205
           VVYR+G  LGE+PEAVRSRHTNLE
Sbjct: 181 VVYRIGVVLGESPEAVRSRHTNLE 201

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BLAST of MS000394 vs. TAIR 10
Match: AT5G52190.1 (Sugar isomerase (SIS) family protein )

HSP 1 Score: 258.1 bits (658), Expect = 6.1e-69
Identity = 131/204 (64.22%), Postives = 163/204 (79.90%), Query Frame = 0


Query: 3   SQSQPSLASKICSDIASIFSKTTHPHAPPMDVLVSELAAAATRK--ARVFLYGVGREGLM 62
           +Q   +L+S+IC+ I+S+F+K T P+ PP+D+LV+EL A +  +  +R+FLYGVGREGLM
Sbjct: 6   AQEMANLSSQICNQISSVFTKPTSPYPPPLDLLVTELTAISRNRNNSRIFLYGVGREGLM 65

Query: 63  LKALCMRLAHLGLSSHQVFDMNAPPIAAQDLLIASAGPGGFSTVDAICSVAKSNGGRVLL 122
           LKA  MRL H GL +H VFDM  PPI++ DLLIASAGPGGFSTVDA+CSVAKSNG +V+L
Sbjct: 66  LKAFAMRLFHFGLPTHLVFDMTTPPISSSDLLIASAGPGGFSTVDALCSVAKSNGAKVIL 125

Query: 123 VTAQPETGSSVKHADVICYVPAQTMADDEEAAAAEGVKSRRPLLPMGSLYEGALFVLFEM 182
           +TA+P  GS VKHA  +CYVPAQTMA D     +  +  RR LLPMGS+YEGALFVLFEM
Sbjct: 126 ITAEPAIGSCVKHATDVCYVPAQTMASDNGGGDSVEMGERR-LLPMGSVYEGALFVLFEM 185

Query: 183 VVYRLGEALGETPEAVRSRHTNLE 205
           VVY+LGE LGE+ E+VR+RHTNLE
Sbjct: 186 VVYKLGEVLGESSESVRARHTNLE 208

The following BLAST results are available for this feature:

BLAST of MS000394 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141151.1	1.3e-105	100.00	uncharacterized protein LOC111011614 [Momordica charantia]	[more]
XP_022945534.1	3.4e-90	86.76	uncharacterized protein LOC111449742 [Cucurbita moschata] >XP_023542323.1 unchar...	[more]
XP_022968332.1	1.3e-89	86.27	uncharacterized protein LOC111467597 [Cucurbita maxima]	[more]
XP_038901080.1	2.2e-89	87.80	3-hexulose-6-phosphate isomerase [Benincasa hispida]	[more]
KAA0033631.1	9.4e-88	85.29	3-hexulose-6-phosphate isomerase [Cucumis melo var. makuwa] >TYK22930.1 3-hexulo...	[more]

BLAST of MS000394 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9YAK0	6.9e-17	32.39	Uncharacterized protein APE_1940.1 OS=Aeropyrum pernix (strain ATCC 700893 / DSM...	[more]
P42404	1.3e-15	31.38	3-hexulose-6-phosphate isomerase OS=Bacillus subtilis (strain 168) OX=224308 GN=...	[more]
O26351	1.1e-14	30.06	Uncharacterized protein MTH_249 OS=Methanothermobacter thermautotrophicus (strai...	[more]
Q9LBW5	1.4e-14	33.73	3-hexulose-6-phosphate isomerase OS=Mycobacterium gastri OX=1777 GN=rmpB PE=2 SV...	[more]
O28478	1.6e-10	28.40	Uncharacterized protein AF_1796 OS=Archaeoglobus fulgidus (strain ATCC 49558 / V...	[more]

BLAST of MS000394 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CI67	6.3e-106	100.00	uncharacterized protein LOC111011614 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1G183	1.7e-90	86.76	uncharacterized protein LOC111449742 OS=Cucurbita moschata OX=3662 GN=LOC1114497...	[more]
A0A6J1HTB3	6.3e-90	86.27	uncharacterized protein LOC111467597 OS=Cucurbita maxima OX=3661 GN=LOC111467597...	[more]
A0A5A7SW26	4.5e-88	85.29	3-hexulose-6-phosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3AXT7	2.9e-87	84.80	3-hexulose-6-phosphate isomerase OS=Cucumis melo OX=3656 GN=LOC103484066 PE=4 SV...	[more]

BLAST of MS000394 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52190.1	6.1e-69	64.22	Sugar isomerase (SIS) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.10490		coord: 11..204 e-value: 1.7E-35 score: 124.1
None	No IPR available	SUPERFAMILY	53697	SIS domain	coord: 31..203
IPR017552	3-hexulose-6-phosphate isomerase	PANTHER	PTHR43443	3-HEXULOSE-6-PHOSPHATE ISOMERASE	coord: 4..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000394.1	MS000394.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016853	isomerase activity