MS000366 (gene) Bitter gourd (TR) v1

Overview
NameMS000366
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionFe2OG dioxygenase domain-containing protein
Locationscaffold44: 1233556 .. 1235625 (+)
RNA-Seq ExpressionMS000366
SyntenyMS000366
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAAACACAGGGGAAGAAGAGAAAAATGGCGGGTAGAAGAGGGGAGGGGGATTCCAAGAAAACAACCCCAACAACGAATTGGCCAGCCATTAAACCCAAGCAGAATCTTCAGATCGTCCGCCTCAAAGAAAATGATCTCTTCACGGTGAGAATCCATATCCAAATTATCTTCAAACTTCCAATCTGTTCTTAGGTTTACATTTGATTCATCTTCGTTCTTATTCAATGCGTCAATTTTTTTCCCTTCCTCTGTTTTTTTGCTCGTTTTTCCCATTTCGTTTGTGGGTGACCTTTTACAATGGAGATAATGGTGGTAAATGGCTTGTTGACGCAATTTGCAATACTTCATACCCTCTTCGATTTTCCATTTCGTGAAATTCTATTACTGAAGAAATTACGTTAGATGTAGATGCTAAACAAAGATTTCATTATAGGGTTTTTGTTAAACAGTGGAGATATGGATCTGTTGTGTATTTTATTTTTTCGACTCTAACTTTCAAACTCAACCGGTTTGGAACATGTATCTGTTAACGAACTAATTCGATATAGGTTACTTCAGTAGTTGGGTTGGCAATGAACGTTATGGTTTAACTTATGTAGGTGCCAAGTTTTCTCACATCTGTTGAGTCAAAAGGATTCATCACGATTGCAGAATCGATGGGTTTTGTTCATCAGGGGAGCCTTGGTCCCACGAAAGGAGAAGCTTTCAGAGATAATGATCGGATCTCGGTGAATGATCCCGATTTAGCAGACACCATTTGGCGTTCGGGACTTGATAAACTATTTGCTGATATTAAAATACGGGGAAAAGTTGCTGTTGGGTTGAATCCAAATATCAGATTTTACAGGTGACTTCAGCCTTACTTTCTAATCAGGCTCTTAACCATTGGGTTGATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTGAAGTTGTTCTAGAAACTATTTCAAGTTTCTAAAAGATTGCATGCGAATACACCAATACTTGGCCAAGTGGTCATCGGAGCAATGAAAAATGTTAATGTTAAGAGCTGGGAGAGAATAGATTCAAATCATGGTGGCCACCTACCTAAGATTTAATGTCTTATAAATTTTCTTGGCAACCAATATAATAGGGTGAGGCAATTGTCTCGTGAGATTAGTTGAAGTGCATAACGAGTTTTCACGTGAATAACATTAGCTATCGCTATGCTTTTAGGGAAGTTTTGGCTCAACATTCAAGTGAGGTGCCTGTGAAGCCATAGATAGTGATAGATGAAATCGATAGGACTAAGCAATCCATGAACTTGGCCTAGTGCAGAGCACATTATATTTAATTTTCCTCCCAAAATTTTTTATGAAGGAGATATGCTTATACTTCTTGGCTTCAAGTTTCAAATTATATTTAGAGTTTTACAAGTCAAGCTATAATGCAGAATTTATTTGATTCTCCTTCTAGGATAAGGCAAGCTTCTTTCTTTGCTGTCGCTTTTGTTTTATAGATTGCAAGATTTGCTATAACTATCTACCGTAGTCTAACACCTAGCCATAACATAAAAACTATGGTATTTCAGATACAATGTTGGTCAACGCTTCGGACGCCATATTGATGAAAGTGTTGATCTTGGAGGGGGAAAGCGCACATATTACACTTTGTTAATATATTTAAGTGGAGGTTCTAAAGGCAAAACAAAAAACGATACCAACGATTCCAAAGGTCCTTCTTCCGAGCCTCTGGTTGGAGGGGAAACCGTTTTCTATGGTTCAAGGAATGGCGTTGTGGCCGAGGTGAATCCCCATTTTTATTTCCAATTGCTTTGCTCCATTTGTTTACAAAGTTTCTGATAATCACACGTGAACTTGCAGAGAGACACCTTATCTGTCTTCCTGTCTTTTCCTTTTCCTGCTACTTCAAGTTCCTTGCATTTTTGTTTTTCTTATGCATTTTCTAGGTGGCTCCTACTGAAGGGATGGCTCTCCTGCACCTTCATGGGGACAAGTGTTTGTTGCATGAAGCTCGCAATGTTACGAAGGGTGTCAAATACATTTTCCGTTCAGATGTCACATTTTGT

mRNA sequence

ATGGCGGAAACACAGGGGAAGAAGAGAAAAATGGCGGGTAGAAGAGGGGAGGGGGATTCCAAGAAAACAACCCCAACAACGAATTGGCCAGCCATTAAACCCAAGCAGAATCTTCAGATCGTCCGCCTCAAAGAAAATGATCTCTTCACGGTGCCAAGTTTTCTCACATCTGTTGAGTCAAAAGGATTCATCACGATTGCAGAATCGATGGGTTTTGTTCATCAGGGGAGCCTTGGTCCCACGAAAGGAGAAGCTTTCAGAGATAATGATCGGATCTCGGTGAATGATCCCGATTTAGCAGACACCATTTGGCGTTCGGGACTTGATAAACTATTTGCTGATATTAAAATACGGGGAAAAGTTGCTGTTGGGTTGAATCCAAATATCAGATTTTACAGATACAATGTTGGTCAACGCTTCGGACGCCATATTGATGAAAGTGTTGATCTTGGAGGGGGAAAGCGCACATATTACACTTTGTTAATATATTTAAGTGGAGGTTCTAAAGGCAAAACAAAAAACGATACCAACGATTCCAAAGGTCCTTCTTCCGAGCCTCTGGTTGGAGGGGAAACCGTTTTCTATGGTTCAAGGAATGGCGTTGTGGCCGAGGTGGCTCCTACTGAAGGGATGGCTCTCCTGCACCTTCATGGGGACAAGTGTTTGTTGCATGAAGCTCGCAATGTTACGAAGGGTGTCAAATACATTTTCCGTTCAGATGTCACATTTTGT

Coding sequence (CDS)

ATGGCGGAAACACAGGGGAAGAAGAGAAAAATGGCGGGTAGAAGAGGGGAGGGGGATTCCAAGAAAACAACCCCAACAACGAATTGGCCAGCCATTAAACCCAAGCAGAATCTTCAGATCGTCCGCCTCAAAGAAAATGATCTCTTCACGGTGCCAAGTTTTCTCACATCTGTTGAGTCAAAAGGATTCATCACGATTGCAGAATCGATGGGTTTTGTTCATCAGGGGAGCCTTGGTCCCACGAAAGGAGAAGCTTTCAGAGATAATGATCGGATCTCGGTGAATGATCCCGATTTAGCAGACACCATTTGGCGTTCGGGACTTGATAAACTATTTGCTGATATTAAAATACGGGGAAAAGTTGCTGTTGGGTTGAATCCAAATATCAGATTTTACAGATACAATGTTGGTCAACGCTTCGGACGCCATATTGATGAAAGTGTTGATCTTGGAGGGGGAAAGCGCACATATTACACTTTGTTAATATATTTAAGTGGAGGTTCTAAAGGCAAAACAAAAAACGATACCAACGATTCCAAAGGTCCTTCTTCCGAGCCTCTGGTTGGAGGGGAAACCGTTTTCTATGGTTCAAGGAATGGCGTTGTGGCCGAGGTGGCTCCTACTGAAGGGATGGCTCTCCTGCACCTTCATGGGGACAAGTGTTTGTTGCATGAAGCTCGCAATGTTACGAAGGGTGTCAAATACATTTTCCGTTCAGATGTCACATTTTGT

Protein sequence

MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVESKGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSKGPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSDVTFC
Homology
BLAST of MS000366 vs. NCBI nr
Match: XP_022140818.1 (uncharacterized protein LOC111011396 [Momordica charantia])

HSP 1 Score: 497.7 bits (1280), Expect = 5.9e-137
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60
           MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES
Sbjct: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60

Query: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120
           KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK
Sbjct: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120

Query: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180
           VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK
Sbjct: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180

Query: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSD 240
           GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKG+KYIFRSD
Sbjct: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGIKYIFRSD 240

Query: 241 VTFC 245
           VTFC
Sbjct: 241 VTFC 244

BLAST of MS000366 vs. NCBI nr
Match: XP_038874454.1 (uncharacterized protein LOC120067107 [Benincasa hispida] >XP_038874463.1 uncharacterized protein LOC120067107 [Benincasa hispida])

HSP 1 Score: 441.0 bits (1133), Expect = 6.5e-120
Identity = 215/243 (88.48%), Postives = 226/243 (93.00%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60
           MAETQGKKRKMAGRR EGD KKT P++ WPAIKPKQNLQI RLK+NDLFTVPSF + VES
Sbjct: 1   MAETQGKKRKMAGRRWEGDFKKTDPSSKWPAIKPKQNLQISRLKDNDLFTVPSFFSCVES 60

Query: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120
           KGFI  AES+GFVHQGSLGPTKGEA+RDNDRISVNDPDLAD IWRSGLD LF+DIKIRGK
Sbjct: 61  KGFIKAAESLGFVHQGSLGPTKGEAYRDNDRISVNDPDLADIIWRSGLDNLFSDIKIRGK 120

Query: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180
           VAVGLNPNIR YRY VGQRFGRHIDESVDLG GKRTYYTLLIYLSGGSK KTKNDTN+SK
Sbjct: 121 VAVGLNPNIRLYRYKVGQRFGRHIDESVDLGEGKRTYYTLLIYLSGGSKNKTKNDTNNSK 180

Query: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSD 240
            PSSEPLVGGETVFYGSRNGVVAEV+PTEGMALLHLHGDKCLLHEARNVT+GVKY+FRSD
Sbjct: 181 DPSSEPLVGGETVFYGSRNGVVAEVSPTEGMALLHLHGDKCLLHEARNVTRGVKYVFRSD 240

Query: 241 VTF 244
           V F
Sbjct: 241 VIF 243

BLAST of MS000366 vs. NCBI nr
Match: XP_022945055.1 (uncharacterized protein LOC111449413 [Cucurbita moschata] >XP_022945056.1 uncharacterized protein LOC111449413 [Cucurbita moschata])

HSP 1 Score: 439.5 bits (1129), Expect = 1.9e-119
Identity = 220/247 (89.07%), Postives = 229/247 (92.71%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTN----WPAIKPKQNLQIVRLKENDLFTVPSFLT 60
           MAETQG+KRKMAGRR EGDSKKT P+++    WPAIKPKQ+LQI RLKENDLFTVPSF T
Sbjct: 1   MAETQGQKRKMAGRRREGDSKKTAPSSHPSSQWPAIKPKQDLQINRLKENDLFTVPSFFT 60

Query: 61  SVESKGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIK 120
           SVESK FI  AESMGFVHQGSLGP+KGEA+RDNDRISVNDPDLADTIWRSGLDK FADIK
Sbjct: 61  SVESKAFIKTAESMGFVHQGSLGPSKGEAYRDNDRISVNDPDLADTIWRSGLDKQFADIK 120

Query: 121 IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDT 180
           IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLG GKRTYYTLLIYLSGGSK KTKNDT
Sbjct: 121 IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGEGKRTYYTLLIYLSGGSKTKTKNDT 180

Query: 181 NDSKGPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYI 240
           N+SK PSSEPLVGGETVFYG RN VVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKY+
Sbjct: 181 NNSKDPSSEPLVGGETVFYGWRNVVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYV 240

Query: 241 FRSDVTF 244
           FRSDV F
Sbjct: 241 FRSDVIF 247

BLAST of MS000366 vs. NCBI nr
Match: XP_023541473.1 (uncharacterized protein LOC111801646 [Cucurbita pepo subsp. pepo] >KAG6574144.1 hypothetical protein SDJN03_28031, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013198.1 hypothetical protein SDJN02_25955, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 437.6 bits (1124), Expect = 7.2e-119
Identity = 219/247 (88.66%), Postives = 228/247 (92.31%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTN----WPAIKPKQNLQIVRLKENDLFTVPSFLT 60
           MAE QG+KRKMAGRR EGDSKKT P+++    WPAIKPKQ+LQI RLKENDLFTVPSF T
Sbjct: 1   MAEAQGQKRKMAGRRREGDSKKTAPSSHPSSQWPAIKPKQDLQINRLKENDLFTVPSFFT 60

Query: 61  SVESKGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIK 120
           SVESK FI  AESMGFVHQGSLGP+KGEA+RDNDRISVNDPDLADTIWRSGLDK FADIK
Sbjct: 61  SVESKAFIKTAESMGFVHQGSLGPSKGEAYRDNDRISVNDPDLADTIWRSGLDKQFADIK 120

Query: 121 IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDT 180
           IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLG GKRTYYTLLIYLSGGSK KTKNDT
Sbjct: 121 IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGEGKRTYYTLLIYLSGGSKTKTKNDT 180

Query: 181 NDSKGPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYI 240
           N+SK PSSEPLVGGETVFYG RN VVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKY+
Sbjct: 181 NNSKDPSSEPLVGGETVFYGWRNVVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYV 240

Query: 241 FRSDVTF 244
           FRSDV F
Sbjct: 241 FRSDVIF 247

BLAST of MS000366 vs. NCBI nr
Match: XP_008439151.1 (PREDICTED: uncharacterized protein LOC103484033 [Cucumis melo])

HSP 1 Score: 437.2 bits (1123), Expect = 9.4e-119
Identity = 212/243 (87.24%), Postives = 223/243 (91.77%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60
           MAETQGKKRKMAGRRGEGD  KT P++NWP IKPKQNLQ+  LK NDLFTVPSF T VES
Sbjct: 1   MAETQGKKRKMAGRRGEGDFNKTDPSSNWPPIKPKQNLQVNLLKGNDLFTVPSFFTCVES 60

Query: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120
           K FI  AES+GF+HQGSLGPTKGEA+RDNDRISVNDPDLAD IWRSGL+ LFADIKIRGK
Sbjct: 61  KAFIKAAESLGFLHQGSLGPTKGEAYRDNDRISVNDPDLADIIWRSGLNNLFADIKIRGK 120

Query: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180
           +AVGLNPNIR YRY VGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSK KTKNDTN+SK
Sbjct: 121 IAVGLNPNIRLYRYKVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKNKTKNDTNNSK 180

Query: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSD 240
            PSSEPLVGGETVFYGSRNGV+AEVAPTEGMALLHLHGDKCLLHEARNV KGVKY+FRSD
Sbjct: 181 DPSSEPLVGGETVFYGSRNGVIAEVAPTEGMALLHLHGDKCLLHEARNVMKGVKYVFRSD 240

Query: 241 VTF 244
           V F
Sbjct: 241 VIF 243

BLAST of MS000366 vs. ExPASy TrEMBL
Match: A0A6J1CIV9 (uncharacterized protein LOC111011396 OS=Momordica charantia OX=3673 GN=LOC111011396 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 2.8e-137
Identity = 243/244 (99.59%), Postives = 244/244 (100.00%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60
           MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES
Sbjct: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60

Query: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120
           KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK
Sbjct: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120

Query: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180
           VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK
Sbjct: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180

Query: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSD 240
           GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKG+KYIFRSD
Sbjct: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGIKYIFRSD 240

Query: 241 VTFC 245
           VTFC
Sbjct: 241 VTFC 244

BLAST of MS000366 vs. ExPASy TrEMBL
Match: A0A6J1FZS7 (uncharacterized protein LOC111449413 OS=Cucurbita moschata OX=3662 GN=LOC111449413 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 9.2e-120
Identity = 220/247 (89.07%), Postives = 229/247 (92.71%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTN----WPAIKPKQNLQIVRLKENDLFTVPSFLT 60
           MAETQG+KRKMAGRR EGDSKKT P+++    WPAIKPKQ+LQI RLKENDLFTVPSF T
Sbjct: 1   MAETQGQKRKMAGRRREGDSKKTAPSSHPSSQWPAIKPKQDLQINRLKENDLFTVPSFFT 60

Query: 61  SVESKGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIK 120
           SVESK FI  AESMGFVHQGSLGP+KGEA+RDNDRISVNDPDLADTIWRSGLDK FADIK
Sbjct: 61  SVESKAFIKTAESMGFVHQGSLGPSKGEAYRDNDRISVNDPDLADTIWRSGLDKQFADIK 120

Query: 121 IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDT 180
           IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLG GKRTYYTLLIYLSGGSK KTKNDT
Sbjct: 121 IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGEGKRTYYTLLIYLSGGSKTKTKNDT 180

Query: 181 NDSKGPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYI 240
           N+SK PSSEPLVGGETVFYG RN VVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKY+
Sbjct: 181 NNSKDPSSEPLVGGETVFYGWRNVVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYV 240

Query: 241 FRSDVTF 244
           FRSDV F
Sbjct: 241 FRSDVIF 247

BLAST of MS000366 vs. ExPASy TrEMBL
Match: A0A1S3AY48 (uncharacterized protein LOC103484033 OS=Cucumis melo OX=3656 GN=LOC103484033 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.5e-119
Identity = 212/243 (87.24%), Postives = 223/243 (91.77%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60
           MAETQGKKRKMAGRRGEGD  KT P++NWP IKPKQNLQ+  LK NDLFTVPSF T VES
Sbjct: 1   MAETQGKKRKMAGRRGEGDFNKTDPSSNWPPIKPKQNLQVNLLKGNDLFTVPSFFTCVES 60

Query: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120
           K FI  AES+GF+HQGSLGPTKGEA+RDNDRISVNDPDLAD IWRSGL+ LFADIKIRGK
Sbjct: 61  KAFIKAAESLGFLHQGSLGPTKGEAYRDNDRISVNDPDLADIIWRSGLNNLFADIKIRGK 120

Query: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180
           +AVGLNPNIR YRY VGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSK KTKNDTN+SK
Sbjct: 121 IAVGLNPNIRLYRYKVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKNKTKNDTNNSK 180

Query: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSD 240
            PSSEPLVGGETVFYGSRNGV+AEVAPTEGMALLHLHGDKCLLHEARNV KGVKY+FRSD
Sbjct: 181 DPSSEPLVGGETVFYGSRNGVIAEVAPTEGMALLHLHGDKCLLHEARNVMKGVKYVFRSD 240

Query: 241 VTF 244
           V F
Sbjct: 241 VIF 243

BLAST of MS000366 vs. ExPASy TrEMBL
Match: A0A0A0L9L9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G179170 PE=4 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.0e-118
Identity = 212/243 (87.24%), Postives = 223/243 (91.77%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTNWPAIKPKQNLQIVRLKENDLFTVPSFLTSVES 60
           MAET GKKRKMAGRRGEGD KKT P++NWP IKPKQNLQ+  LK+NDLFTVPSF T VES
Sbjct: 1   MAETLGKKRKMAGRRGEGDFKKTDPSSNWPPIKPKQNLQVNLLKDNDLFTVPSFFTCVES 60

Query: 61  KGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIKIRGK 120
           K FI  AES+GF+HQGSLGPTKGEA+RDNDRISVNDPDLAD IWRSGLD LFADIKIRGK
Sbjct: 61  KAFIKAAESLGFLHQGSLGPTKGEAYRDNDRISVNDPDLADIIWRSGLDNLFADIKIRGK 120

Query: 121 VAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDTNDSK 180
           VAVGLNPNIR YRY VGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSK KTKNDTN+SK
Sbjct: 121 VAVGLNPNIRLYRYKVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKNKTKNDTNNSK 180

Query: 181 GPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSD 240
            PSS+ LVGGETVFYGSRNGV+AEVAPTEGMALLHLHGDKCLLHEARNV KGVKY+FRSD
Sbjct: 181 DPSSDTLVGGETVFYGSRNGVIAEVAPTEGMALLHLHGDKCLLHEARNVRKGVKYVFRSD 240

Query: 241 VTF 244
           V F
Sbjct: 241 VIF 243

BLAST of MS000366 vs. ExPASy TrEMBL
Match: A0A6J1HX87 (uncharacterized protein LOC111467697 OS=Cucurbita maxima OX=3661 GN=LOC111467697 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.3e-118
Identity = 218/247 (88.26%), Postives = 227/247 (91.90%), Query Frame = 0

Query: 1   MAETQGKKRKMAGRRGEGDSKKTTPTTN----WPAIKPKQNLQIVRLKENDLFTVPSFLT 60
           MAETQG+KRKMAGRR EGDSKKT P+++    WPAIKPKQ+LQI RLKENDLFTVPSF  
Sbjct: 1   MAETQGQKRKMAGRRREGDSKKTAPSSHPSSQWPAIKPKQDLQINRLKENDLFTVPSFFA 60

Query: 61  SVESKGFITIAESMGFVHQGSLGPTKGEAFRDNDRISVNDPDLADTIWRSGLDKLFADIK 120
           SVESK FI  AESMGFVHQGSLGP+KGEA+RDNDRISVNDPDLADTIWRSGLDK FADIK
Sbjct: 61  SVESKAFIKTAESMGFVHQGSLGPSKGEAYRDNDRISVNDPDLADTIWRSGLDKHFADIK 120

Query: 121 IRGKVAVGLNPNIRFYRYNVGQRFGRHIDESVDLGGGKRTYYTLLIYLSGGSKGKTKNDT 180
           IRGKVAVGLNPNIRFYRY VGQRFGRHIDESVDLG GKRTYYTLLIYLSGGSK KTKNDT
Sbjct: 121 IRGKVAVGLNPNIRFYRYKVGQRFGRHIDESVDLGEGKRTYYTLLIYLSGGSKTKTKNDT 180

Query: 181 NDSKGPSSEPLVGGETVFYGSRNGVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYI 240
           N+SK PSSEPLVGGETVFYG RN VVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKY+
Sbjct: 181 NNSKDPSSEPLVGGETVFYGWRNVVVAEVAPTEGMALLHLHGDKCLLHEARNVTKGVKYV 240

Query: 241 FRSDVTF 244
           FRSDV F
Sbjct: 241 FRSDVIF 247

BLAST of MS000366 vs. TAIR 10
Match: AT5G51880.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 2.7e-87
Identity = 159/219 (72.60%), Postives = 176/219 (80.37%), Query Frame = 0

Query: 29  WPAIKPKQNLQIVRLKENDLFTVPSFLTSVESKGFITIAESMGFVHQGSLGPTKGEAFRD 88
           WP IK K NL +  LK +DLFTV + LTS ESK F+ IAES+GF HQGS GP  GEA+RD
Sbjct: 33  WPKIKLKSNLNVSYLKNHDLFTVENCLTSDESKAFVKIAESLGFTHQGSRGPAYGEAYRD 92

Query: 89  NDRISVNDPDLADTIWRSGLDKLFADIKIRGKVAVGLNPNIRFYRYNVGQRFGRHIDESV 148
           N RISVNDP LADT+W+SGL  LF DIKIR KVAVGLNPNIRFYRY+ GQ FGRHIDES 
Sbjct: 93  NHRISVNDPVLADTLWQSGLSNLFTDIKIRRKVAVGLNPNIRFYRYSAGQHFGRHIDESA 152

Query: 149 DLGGGKRTYYTLLIYLSGGS-KGKTKND---TNDSKGPSSEPLVGGETVFYGSRNGVVAE 208
           DL  G RTYYTLLIYLSG S K K+K+    TNDS   S+EPLVGGETVFYGSRN +VAE
Sbjct: 153 DLEDGNRTYYTLLIYLSGNSTKSKSKSSSSKTNDSS--SAEPLVGGETVFYGSRNSIVAE 212

Query: 209 VAPTEGMALLHLHGDKCLLHEARNVTKGVKYIFRSDVTF 244
           VAP EGMAL H+HGDKC+LHE RNV+KGVKY+FRSDV F
Sbjct: 213 VAPVEGMALFHIHGDKCMLHEGRNVSKGVKYVFRSDVVF 249

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140818.15.9e-13799.59uncharacterized protein LOC111011396 [Momordica charantia][more]
XP_038874454.16.5e-12088.48uncharacterized protein LOC120067107 [Benincasa hispida] >XP_038874463.1 unchara... [more]
XP_022945055.11.9e-11989.07uncharacterized protein LOC111449413 [Cucurbita moschata] >XP_022945056.1 unchar... [more]
XP_023541473.17.2e-11988.66uncharacterized protein LOC111801646 [Cucurbita pepo subsp. pepo] >KAG6574144.1 ... [more]
XP_008439151.19.4e-11987.24PREDICTED: uncharacterized protein LOC103484033 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1CIV92.8e-13799.59uncharacterized protein LOC111011396 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A6J1FZS79.2e-12089.07uncharacterized protein LOC111449413 OS=Cucurbita moschata OX=3662 GN=LOC1114494... [more]
A0A1S3AY484.5e-11987.24uncharacterized protein LOC103484033 OS=Cucumis melo OX=3656 GN=LOC103484033 PE=... [more]
A0A0A0L9L91.0e-11887.24Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6J1HX872.3e-11888.26uncharacterized protein LOC111467697 OS=Cucurbita maxima OX=3661 GN=LOC111467697... [more]
Match NameE-valueIdentityDescription
AT5G51880.12.7e-8772.602-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 46..242
e-value: 1.1E-24
score: 98.0
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 35..239
e-value: 6.6E-29
score: 103.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR10869:SF1492-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 16..243
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 16..243
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 124..244
score: 8.911905

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000366.1MS000366.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen