MS000364 (gene) Bitter gourd (TR) v1

BLAST of MS000364 vs. NCBI nr
Match: XP_022140849.1 (uncharacterized protein LOC111011419 isoform X1 [Momordica charantia])

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 574/578 (99.31%), Postives = 574/578 (99.31%), Query Frame = 0

Query: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ 60
           MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ
Sbjct: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ 60

Query: 61  SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120
           SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV
Sbjct: 61  SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120

Query: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT-SSSVS 180
           QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT SSSVS
Sbjct: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSSSVS 180

Query: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240
           PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY
Sbjct: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240

Query: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300
           AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN
Sbjct: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300

Query: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSNELL 360
           LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKA IDSPRKKSKKTPLSNELL
Sbjct: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKATIDSPRKKSKKTPLSNELL 360

Query: 361 SFSSVPTVVKKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420
           SFSSVPTVV KKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV
Sbjct: 361 SFSSVPTVVMKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420

Query: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480
           IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL
Sbjct: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480

Query: 481 LCHLAEELLKPYVDTMMRPWAGGGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 540
           LCHLAEELLKPYVDTMMRPWAG GWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR
Sbjct: 481 LCHLAEELLKPYVDTMMRPWAGVGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 540

Query: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 578
           LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV
Sbjct: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 578

BLAST of MS000364 vs. NCBI nr
Match: XP_022140850.1 (uncharacterized protein LOC111011419 isoform X2 [Momordica charantia])

HSP 1 Score: 1009.2 bits (2608), Expect = 1.4e-290
Identity = 531/578 (91.87%), Postives = 531/578 (91.87%), Query Frame = 0

Query: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ 60
           MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGC               
Sbjct: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGC--------------- 60

Query: 61  SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120
                                       TEAPRNSLESDEELPSPCTKQKDGLHIPEGIV
Sbjct: 61  ----------------------------TEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120

Query: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT-SSSVS 180
           QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT SSSVS
Sbjct: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSSSVS 180

Query: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240
           PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY
Sbjct: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240

Query: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300
           AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN
Sbjct: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300

Query: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSNELL 360
           LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKA IDSPRKKSKKTPLSNELL
Sbjct: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKATIDSPRKKSKKTPLSNELL 360

Query: 361 SFSSVPTVVKKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420
           SFSSVPTVV KKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV
Sbjct: 361 SFSSVPTVVMKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420

Query: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480
           IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL
Sbjct: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480

Query: 481 LCHLAEELLKPYVDTMMRPWAGGGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 540
           LCHLAEELLKPYVDTMMRPWAG GWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR
Sbjct: 481 LCHLAEELLKPYVDTMMRPWAGVGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 535

Query: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 578
           LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV
Sbjct: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 535

BLAST of MS000364 vs. NCBI nr
Match: XP_023541466.1 (uncharacterized protein LOC111801640 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.9 bits (1439), Expect = 5.1e-155
Identity = 357/603 (59.20%), Postives = 423/603 (70.15%), Query Frame = 0

Query: 1   MGKQR-YWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLN 60
           MGKQR   +GH   T R       +N + +    DH EASSTSAGCMCAVF LFDFHPLN
Sbjct: 16  MGKQRSSTAGHAGRTAR-------ANKDPNGVLDDH-EASSTSAGCMCAVFHLFDFHPLN 75

Query: 61  QSHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTK--QKDGLHIPE 120
           Q HHHPP  S  VSL P+    DH I TGTEAPRNSLES+EE P  CT+  ++D LH P+
Sbjct: 76  QHHHHPPPPSHPVSLNPL----DHRISTGTEAPRNSLESEEESPLSCTRKQKQDALHFPK 135

Query: 121 GIVQIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSS 180
           G++QIKT+      +N   + FSG+SPSTKTPTLVARLMGLDLLP+S+SPSTTPRT  +S
Sbjct: 136 GVLQIKTKG---SRNNEAKEKFSGDSPSTKTPTLVARLMGLDLLPQSNSPSTTPRT--AS 195

Query: 181 VSPI-YPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKEN---S 240
            SP+ YP+L K + +    SRSLPETPR+SCER+SN  +NYH RLSLQIPNYDKEN   S
Sbjct: 196 ASPLNYPILHKQQRT---ASRSLPETPRSSCERRSNV-ENYHPRLSLQIPNYDKENASPS 255

Query: 241 TSPSHYAKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVL 300
            SPSHYAKEIVKQIKES+SRK GL DIT NY RRDH+++NQ KP+K+ S SS+SP+ RV+
Sbjct: 256 PSPSHYAKEIVKQIKESMSRKGGLNDIT-NYARRDHEMVNQTKPRKVLSCSSVSPRQRVV 315

Query: 301 EAKNTNLKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTP 360
           E KN N      PK + I     + A  KK NKQEE FVVPSR+TKAAIDSP    KKTP
Sbjct: 316 EPKNLN-----KPKQKVI-----SKAALKKSNKQEEPFVVPSRITKAAIDSP---LKKTP 375

Query: 361 LSNELLSFSSVPTVVKKKE------AKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTF 420
           LS++LLSFSSVPTVV KK        KP PMQ   CNR++ A   TGDKESK  LQ S+ 
Sbjct: 376 LSDKLLSFSSVPTVVIKKNPPFSSPIKPTPMQPPPCNRDQAAGQ-TGDKESKRCLQ-SSN 435

Query: 421 HNNHKTL--HHQHVIQLPNNNKHHIISAAA-----DRHDITAEVDCVRKIL----TTRTN 480
           H +H  +  HHQHVIQLP  N  +I +A+A     + HDITAEV+ +R+IL    TT T+
Sbjct: 436 HQSHFPIHHHHQHVIQLPPINNINITTASAAANRRNHHDITAEVEYIRQILLRRSTTSTS 495

Query: 481 -----NFLENNTTQKEVPIWHPHYDTKLLCHLAEELLKPYVDTMMRPW---AGGGWPALV 540
                NFLEN  T   + I H     KLL HL EELLKPY++   RP+   A   W  +V
Sbjct: 496 VYSSVNFLENGNTHHNISITH----RKLLSHLVEELLKPYIE--RRPYRRSAAERWAEVV 555

Query: 541 EELCDKVRRYPRARCEVLEDIDGIIEKDMDRLGMGFEEEGEGIVTQIEEGIVEELLKETV 572
           E++C+KVR++PRA+CEVLEDIDGIIEKDMD LG+GFEEE EGIV  IEE +VEELL ETV
Sbjct: 556 EKVCEKVRKFPRAKCEVLEDIDGIIEKDMDILGIGFEEEDEGIVKDIEEWVVEELLNETV 575

BLAST of MS000364 vs. NCBI nr
Match: XP_022968601.1 (uncharacterized protein LOC111467773 [Cucurbita maxima])

HSP 1 Score: 554.7 bits (1428), Expect = 9.5e-154
Identity = 356/597 (59.63%), Postives = 416/597 (69.68%), Query Frame = 0

Query: 1   MGKQR-YWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLN 60
           MGKQR   +GH   T R       +N + +    DH EASSTSAGCMCAVF LFDFHPLN
Sbjct: 16  MGKQRSSTAGHAGRTAR-------ANKDPNGVIDDH-EASSTSAGCMCAVFHLFDFHPLN 75

Query: 61  QSHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTK--QKDGLHIPE 120
           Q HHHPP  S  VSL P+    DH I TGTEAPRNSLESDEE P  CT+  ++D LH P+
Sbjct: 76  Q-HHHPPPPSRPVSLNPL----DHRISTGTEAPRNSLESDEESPLSCTRKQKQDALHFPK 135

Query: 121 GIVQIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSS 180
           G++QIKT+      +N   + FSG+SPSTKTPTLVARLMGLDLLP+S+SPSTTPRT S S
Sbjct: 136 GVLQIKTKG---SPNNEAKEKFSGDSPSTKTPTLVARLMGLDLLPQSNSPSTTPRTASVS 195

Query: 181 VSPIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKEN-STSP 240
            S  YP+L K + +    SRSLPETPR+SCER+SN  +NYH RLSLQIPNYDKEN S SP
Sbjct: 196 -SLNYPILHKQQRT---ASRSLPETPRSSCERRSNV-ENYHPRLSLQIPNYDKENASPSP 255

Query: 241 SHYAKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAK 300
           SHYAKEIVKQIKES+SRK GL DIT NY RRDH+++NQ KP+K+ SSSS+SP+ RV+E K
Sbjct: 256 SHYAKEIVKQIKESMSRKGGLNDIT-NYARRDHEMVNQTKPRKVLSSSSVSPRQRVVEPK 315

Query: 301 NTNLKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSN 360
           N N    P  KV       K+ A  KK NKQEE FVVPSR+TKAAIDSP    KKTPLS+
Sbjct: 316 NLN---KPKQKV-------KSKAALKKSNKQEEPFVVPSRITKAAIDSP---LKKTPLSD 375

Query: 361 ELLSFSSVPTVVKKKE------AKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNN 420
           +LLSFSSVPTVV KK        KP PMQ   CNRN+ A   TG KESK  LQ S   ++
Sbjct: 376 KLLSFSSVPTVVMKKNPPFSSPIKPTPMQPPPCNRNQAA-VQTGGKESKRCLQSSNHQSH 435

Query: 421 HKTL-HHQHVIQLPNNNKHHIISAAA-----DRHDITAEVDCVRKIL----TTRTN---- 480
              L HHQHVIQLP  N  +I +A+A     + HDITAEV+ VR+IL    TT T+    
Sbjct: 436 FPILHHHQHVIQLPPINNINITTASAASNRRNHHDITAEVEYVRQILLRRSTTSTSVYSS 495

Query: 481 -NFLENNTTQKEVPIWHPHYDTKLLCHLAEELLKPYVDTM-MRPWAGGGWPALVEELCDK 540
            NFLEN  T   + I H     KLL HL EELLKPY++    R  A   W  +VE++C+K
Sbjct: 496 VNFLENCNTHHNISITH----RKLLSHLVEELLKPYIERRPYRRTAAERWAEVVEKVCEK 555

Query: 541 VRRYPRARCEVLEDIDGIIEKDMDRLGMGFEEEGEGIVTQIEEGIVEELLKETVRFV 572
           V ++PRA+CEV+EDIDGIIEKDMD LG+GFEE+ EGIV  IEE +VEELL ETVRFV
Sbjct: 556 VGKFPRAKCEVMEDIDGIIEKDMDILGIGFEEDDEGIVKDIEEWVVEELLNETVRFV 572

BLAST of MS000364 vs. NCBI nr
Match: KAG6574143.1 (hypothetical protein SDJN03_28030, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554.3 bits (1427), Expect = 1.2e-153
Identity = 357/601 (59.40%), Postives = 420/601 (69.88%), Query Frame = 0

Query: 1   MGKQR-YWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLN 60
           MGKQR   +GHG  T R       +N + +    DH EASSTSAGCMCAVF LFDFHPLN
Sbjct: 12  MGKQRSSTAGHGGRTAR-------ANKDPNGVLDDH-EASSTSAGCMCAVFHLFDFHPLN 71

Query: 61  QSHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTK--QKDGLHIPE 120
           Q HHHPP  S  VSL P+    DH I TGTEAPRNSLES+EE P  CT+  ++D LH P+
Sbjct: 72  Q-HHHPPPPSRPVSLNPL----DHRISTGTEAPRNSLESEEESPLSCTRNQKQDALHFPK 131

Query: 121 GIVQIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSS 180
           G++QIKT+      +N   +  SG+SPSTKTPTLVARLMGLDLLP+S+SPSTTPRT  +S
Sbjct: 132 GVLQIKTKG---SRNNEAKEKLSGDSPSTKTPTLVARLMGLDLLPQSNSPSTTPRT--AS 191

Query: 181 VSPI-YPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKEN---S 240
            SP+ YP+L K + +    SRSLPETPR+SCER+SN  +NYH RLSLQIPNYDKEN   S
Sbjct: 192 ASPLNYPILHKQQRT---ASRSLPETPRSSCERRSNV-ENYHPRLSLQIPNYDKENASPS 251

Query: 241 TSPSHYAKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVL 300
            SPSH+AKEIVKQIKES+SRK GL DIT NY RRDH+++NQ KP+K+ S SS+SP+ RV+
Sbjct: 252 PSPSHHAKEIVKQIKESMSRKGGLDDIT-NYARRDHEMVNQTKPRKVLSCSSVSPRQRVV 311

Query: 301 EAKNTNLKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTP 360
           E KN N    P  KV       K+ A  KK NKQEE FVVPSR+TKAAIDSP    KKTP
Sbjct: 312 EPKNLN---KPKQKV-------KSKAALKKSNKQEEPFVVPSRITKAAIDSP---LKKTP 371

Query: 361 LSNELLSFSSVPTVVKKKE------AKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTF 420
           LS++LLSFSSVPTVV KK        KP PMQ   CNRN+ A    GDKESK  LQ S+ 
Sbjct: 372 LSDKLLSFSSVPTVVVKKNPPFSSPIKPTPMQPPPCNRNQAA-VQRGDKESKRCLQ-SSN 431

Query: 421 HNNHKTL--HHQHVIQLPNNNKHHIISAAA-----DRHDITAEVDCVRKIL----TTRTN 480
           H +H  +  HHQHVIQLP  N  +I +A+A     + HDITAEV+ +RKIL    TT T+
Sbjct: 432 HQSHFPIHHHHQHVIQLPPINNINITTASAAANRRNHHDITAEVEYIRKILLRRSTTSTS 491

Query: 481 -----NFLENNTTQKEVPIWHPHYDTKLLCHLAEELLKPYVDTM-MRPWAGGGWPALVEE 540
                NFLEN  T   + I H     KLL HL EELLKPY++    R  A   W  +VE+
Sbjct: 492 VYSSVNFLENCNTHHNISITH----RKLLSHLVEELLKPYIERRPYRRTAAERWAEVVEK 551

Query: 541 LCDKVRRYPRARCEVLEDIDGIIEKDMDRLGMGFEEEGEGIVTQIEEGIVEELLKETVRF 572
           +C+KVR++PRA+CEVLEDIDGIIEKDMD LG+GFEEE EGIV  IEE +VEELL ETVRF
Sbjct: 552 VCEKVRKFPRAKCEVLEDIDGIIEKDMDILGIGFEEEDEGIVKDIEEWVVEELLNETVRF 570

BLAST of MS000364 vs. ExPASy TrEMBL
Match: A0A6J1CGX4 (uncharacterized protein LOC111011419 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011419 PE=4 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 574/578 (99.31%), Postives = 574/578 (99.31%), Query Frame = 0

Query: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ 60
           MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ
Sbjct: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ 60

Query: 61  SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120
           SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV
Sbjct: 61  SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120

Query: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT-SSSVS 180
           QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT SSSVS
Sbjct: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSSSVS 180

Query: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240
           PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY
Sbjct: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240

Query: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300
           AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN
Sbjct: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300

Query: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSNELL 360
           LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKA IDSPRKKSKKTPLSNELL
Sbjct: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKATIDSPRKKSKKTPLSNELL 360

Query: 361 SFSSVPTVVKKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420
           SFSSVPTVV KKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV
Sbjct: 361 SFSSVPTVVMKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420

Query: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480
           IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL
Sbjct: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480

Query: 481 LCHLAEELLKPYVDTMMRPWAGGGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 540
           LCHLAEELLKPYVDTMMRPWAG GWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR
Sbjct: 481 LCHLAEELLKPYVDTMMRPWAGVGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 540

Query: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 578
           LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV
Sbjct: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 578

BLAST of MS000364 vs. ExPASy TrEMBL
Match: A0A6J1CGB7 (uncharacterized protein LOC111011419 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011419 PE=4 SV=1)

HSP 1 Score: 1009.2 bits (2608), Expect = 6.9e-291
Identity = 531/578 (91.87%), Postives = 531/578 (91.87%), Query Frame = 0

Query: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQ 60
           MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGC               
Sbjct: 1   MGKQRYWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGC--------------- 60

Query: 61  SHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120
                                       TEAPRNSLESDEELPSPCTKQKDGLHIPEGIV
Sbjct: 61  ----------------------------TEAPRNSLESDEELPSPCTKQKDGLHIPEGIV 120

Query: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT-SSSVS 180
           QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT SSSVS
Sbjct: 121 QIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSSSVS 180

Query: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240
           PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY
Sbjct: 181 PIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKENSTSPSHY 240

Query: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300
           AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN
Sbjct: 241 AKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNTN 300

Query: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSNELL 360
           LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKA IDSPRKKSKKTPLSNELL
Sbjct: 301 LKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKATIDSPRKKSKKTPLSNELL 360

Query: 361 SFSSVPTVVKKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420
           SFSSVPTVV KKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV
Sbjct: 361 SFSSVPTVVMKKEAKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHV 420

Query: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480
           IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL
Sbjct: 421 IQLPNNNKHHIISAAADRHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWHPHYDTKL 480

Query: 481 LCHLAEELLKPYVDTMMRPWAGGGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 540
           LCHLAEELLKPYVDTMMRPWAG GWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR
Sbjct: 481 LCHLAEELLKPYVDTMMRPWAGVGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDR 535

Query: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 578
           LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV
Sbjct: 541 LGMGFEEEGEGIVTQIEEGIVEELLKETVRFVEPDGVV 535

BLAST of MS000364 vs. ExPASy TrEMBL
Match: A0A6J1HVD0 (uncharacterized protein LOC111467773 OS=Cucurbita maxima OX=3661 GN=LOC111467773 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 4.6e-154
Identity = 356/597 (59.63%), Postives = 416/597 (69.68%), Query Frame = 0

Query: 1   MGKQR-YWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLN 60
           MGKQR   +GH   T R       +N + +    DH EASSTSAGCMCAVF LFDFHPLN
Sbjct: 16  MGKQRSSTAGHAGRTAR-------ANKDPNGVIDDH-EASSTSAGCMCAVFHLFDFHPLN 75

Query: 61  QSHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTK--QKDGLHIPE 120
           Q HHHPP  S  VSL P+    DH I TGTEAPRNSLESDEE P  CT+  ++D LH P+
Sbjct: 76  Q-HHHPPPPSRPVSLNPL----DHRISTGTEAPRNSLESDEESPLSCTRKQKQDALHFPK 135

Query: 121 GIVQIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSS 180
           G++QIKT+      +N   + FSG+SPSTKTPTLVARLMGLDLLP+S+SPSTTPRT S S
Sbjct: 136 GVLQIKTKG---SPNNEAKEKFSGDSPSTKTPTLVARLMGLDLLPQSNSPSTTPRTASVS 195

Query: 181 VSPIYPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKEN-STSP 240
            S  YP+L K + +    SRSLPETPR+SCER+SN  +NYH RLSLQIPNYDKEN S SP
Sbjct: 196 -SLNYPILHKQQRT---ASRSLPETPRSSCERRSNV-ENYHPRLSLQIPNYDKENASPSP 255

Query: 241 SHYAKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAK 300
           SHYAKEIVKQIKES+SRK GL DIT NY RRDH+++NQ KP+K+ SSSS+SP+ RV+E K
Sbjct: 256 SHYAKEIVKQIKESMSRKGGLNDIT-NYARRDHEMVNQTKPRKVLSSSSVSPRQRVVEPK 315

Query: 301 NTNLKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSN 360
           N N    P  KV       K+ A  KK NKQEE FVVPSR+TKAAIDSP    KKTPLS+
Sbjct: 316 NLN---KPKQKV-------KSKAALKKSNKQEEPFVVPSRITKAAIDSP---LKKTPLSD 375

Query: 361 ELLSFSSVPTVVKKKE------AKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTFHNN 420
           +LLSFSSVPTVV KK        KP PMQ   CNRN+ A   TG KESK  LQ S   ++
Sbjct: 376 KLLSFSSVPTVVMKKNPPFSSPIKPTPMQPPPCNRNQAA-VQTGGKESKRCLQSSNHQSH 435

Query: 421 HKTL-HHQHVIQLPNNNKHHIISAAA-----DRHDITAEVDCVRKIL----TTRTN---- 480
              L HHQHVIQLP  N  +I +A+A     + HDITAEV+ VR+IL    TT T+    
Sbjct: 436 FPILHHHQHVIQLPPINNINITTASAASNRRNHHDITAEVEYVRQILLRRSTTSTSVYSS 495

Query: 481 -NFLENNTTQKEVPIWHPHYDTKLLCHLAEELLKPYVDTM-MRPWAGGGWPALVEELCDK 540
            NFLEN  T   + I H     KLL HL EELLKPY++    R  A   W  +VE++C+K
Sbjct: 496 VNFLENCNTHHNISITH----RKLLSHLVEELLKPYIERRPYRRTAAERWAEVVEKVCEK 555

Query: 541 VRRYPRARCEVLEDIDGIIEKDMDRLGMGFEEEGEGIVTQIEEGIVEELLKETVRFV 572
           V ++PRA+CEV+EDIDGIIEKDMD LG+GFEE+ EGIV  IEE +VEELL ETVRFV
Sbjct: 556 VGKFPRAKCEVMEDIDGIIEKDMDILGIGFEEDDEGIVKDIEEWVVEELLNETVRFV 572

BLAST of MS000364 vs. ExPASy TrEMBL
Match: A0A6J1FZQ2 (uncharacterized protein LOC111449407 OS=Cucurbita moschata OX=3662 GN=LOC111449407 PE=4 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 8.7e-153
Identity = 355/601 (59.07%), Postives = 419/601 (69.72%), Query Frame = 0

Query: 1   MGKQR-YWSGHGRATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLN 60
           MGKQR   +GHG  T R       +N + +    DH EASSTSAGCMCAVF LFDFHPLN
Sbjct: 16  MGKQRSSTAGHGGRTAR-------ANKDPNGVLDDH-EASSTSAGCMCAVFHLFDFHPLN 75

Query: 61  QSHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTK--QKDGLHIPE 120
           Q HHHPP  S  VSL P+    DH I TGTEAPRNSLES+EE P  CT+  ++D LH P+
Sbjct: 76  Q-HHHPPPPSRPVSLNPL----DHRISTGTEAPRNSLESEEESPLSCTRNQKQDALHFPK 135

Query: 121 GIVQIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSS 180
           G++QIKT+      +N   +  SG+SPSTKTPTLVARLMGLDLLP+S+SPSTTPRT  +S
Sbjct: 136 GVLQIKTKG---SRNNEAKEKLSGDSPSTKTPTLVARLMGLDLLPQSNSPSTTPRT--AS 195

Query: 181 VSPI-YPLLQKPKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIPNYDKEN---S 240
            SP+ YP+L K + +    SRSLPETPR+SCER+SN  +NYH RLSLQIPNYDKEN   S
Sbjct: 196 ASPLNYPILHKQQRT---ASRSLPETPRSSCERRSNV-ENYHPRLSLQIPNYDKENASPS 255

Query: 241 TSPSHYAKEIVKQIKESVSRKSGLTDITNNYQRRDHDVMNQPKPKKISSSSSLSPKLRVL 300
            SPSH+AKEIVKQIKES+SRK GL DIT NY RRDH+++NQ KP+K+ S SS+SP+ RV+
Sbjct: 256 PSPSHHAKEIVKQIKESMSRKGGLDDIT-NYARRDHEMVNQTKPRKVLSCSSVSPRQRVV 315

Query: 301 EAKNTNLKKLPSPKVEAIKGSKKAPAPAKKGNKQEEAFVVPSRMTKAAIDSPRKKSKKTP 360
           E KN N    P  KV       K+ A  KK NKQEE FVVPSR+TKAAIDSP    KKTP
Sbjct: 316 EPKNLN---KPKQKV-------KSKAALKKSNKQEEPFVVPSRITKAAIDSP---LKKTP 375

Query: 361 LSNELLSFSSVPTVVKKKE------AKPPPMQSGACNRNEGAETVTGDKESKPVLQLSTF 420
           LS++LLSFSSVPTVV KK        KP PMQ   CNRN+ A   TGDKESK  LQ S+ 
Sbjct: 376 LSDKLLSFSSVPTVVIKKNPPFSSPIKPTPMQPPPCNRNQAA-VQTGDKESKRCLQ-SSN 435

Query: 421 HNNHKTL--HHQHVIQLPNNNKHHIISAAA-----DRHDITAEVDCVRKIL----TTRTN 480
           H +H  +  HHQHVIQLP  N  +I +A+A     + HDITAEV+ +R+IL    TT T+
Sbjct: 436 HQSHFPIHHHHQHVIQLPPINNINITTASAAANRRNHHDITAEVEYIRQILLRRSTTSTS 495

Query: 481 -----NFLENNTTQKEVPIWHPHYDTKLLCHLAEELLKPYVDTM-MRPWAGGGWPALVEE 540
                NFLEN  T   + I H     KLL HL EELLKPY++    R  A   W  +VE+
Sbjct: 496 VYSSVNFLENCNTHHNISITH----RKLLSHLVEELLKPYIERRPYRRTAAERWAEVVEK 555

Query: 541 LCDKVRRYPRARCEVLEDIDGIIEKDMDRLGMGFEEEGEGIVTQIEEGIVEELLKETVRF 572
           +C+KVR++PRA+CEVLEDIDGIIEKDMD LG+GFEEE EG V  IEE +VEELL ETVR 
Sbjct: 556 VCEKVRKFPRAKCEVLEDIDGIIEKDMDILGIGFEEEDEGTVKDIEEWVVEELLNETVRL 574

BLAST of MS000364 vs. ExPASy TrEMBL
Match: A0A6J1J8L6 (uncharacterized protein LOC111482735 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482735 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 1.6e-151
Identity = 375/646 (58.05%), Postives = 438/646 (67.80%), Query Frame = 0

Query: 1   MGKQRYWSGHGR----ATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFH 60
           MGKQRYWSGHGR    +T+ LKNE           +   E +SSTSAGCMCAVFQLFDFH
Sbjct: 1   MGKQRYWSGHGRTATASTKTLKNE----------PNGFDEASSSTSAGCMCAVFQLFDFH 60

Query: 61  PL-NQSHHHPPQSSLSVSLKPISLPP---DHHIPTGTEAPRNSLESDEELPS-PCT-KQK 120
           PL +  HHHPP+S  +VSL PI  PP   D H+  GTEAPRNS+ES+EE  S PCT KQK
Sbjct: 61  PLSHHQHHHPPRS--TVSLNPIVSPPPPDDDHLSKGTEAPRNSVESEEEESSLPCTPKQK 120

Query: 121 -DGLHIPEGIVQIKTRSR-SKDSSNSDGDNFS--GNSPSTKTPTLVARLMGLDLLPESSS 180
            DGLH P+GIVQIKT+S  +K+S  +   N S   +SPSTKTPTLVARLMGLDLLP+S+S
Sbjct: 121 EDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMGLDLLPQSTS 180

Query: 181 PSTTPRTTSSSVSPIYPLLQKPKYSKTGV--SRSLPETPRTSCERKSNADQNYHHRLSLQ 240
           PSTT RT        Y L+ KPK  K  +  +RSLPETPRTSCERK N D NYHHRLSLQ
Sbjct: 181 PSTTTRTP-------YSLVGKPKTGKNHLTGTRSLPETPRTSCERKPNVD-NYHHRLSLQ 240

Query: 241 IPNYDKENST-----SPSHYAKEIVKQIKESVSRKSGLTDITNNY--QRRDHDVMNQPKP 300
           IPN+DKEN+T     +PSHYAKEIVKQIKE+VSRK GL+DITNNY   RRD D++ Q KP
Sbjct: 241 IPNFDKENATPSPSPNPSHYAKEIVKQIKETVSRKGGLSDITNNYYSTRRDQDMITQTKP 300

Query: 301 KKI---SSSSSLSPKLRVLEAKNTNLKKLPSPKVEAIK----GSK--------KAPAPAK 360
           KK+   SSSSS+SP+L     KN N    P+PKVEA+K    G +        K  A  K
Sbjct: 301 KKVLSLSSSSSVSPRL----PKNPN---KPTPKVEAMKEGVLGQRKPRTQKPVKTKAGLK 360

Query: 361 KGNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSNELLSFSSVPTVVKKKE------AKPP 420
           K NKQEE FVVPSR+TKAAID P K +KKTPLSN+LLSF SVPT++ KK+       KP 
Sbjct: 361 KSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLSFGSVPTILMKKDPPFPSPIKPF 420

Query: 421 PMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTL--HHQHVIQLP--------NNN 480
           PMQS  CNRN+ A   T DKESK  LQ S+ H  H  +  HHQHVIQLP        NN 
Sbjct: 421 PMQS-PCNRNQPANQ-TVDKESKRYLQ-SSNHQPHIPIIHHHQHVIQLPLSNSNKDGNNP 480

Query: 481 KHHIISAAAD---RHDITAEVDCVRKILTTRTNN--------FLEN-NTTQKEVPIWHPH 540
           K +  +AAAD    HDI AE++ VR+IL  R +         F EN NTT   V   H  
Sbjct: 481 KQN--AAAADCSGNHDIAAELEYVRQILLRRRSTSTSVYSTVFPENYNTTHNRVSNSH-- 540

Query: 541 YDTKLLCHLAEELLKPYVDTMMRPWAGGG-----WPALVEELCDKVRRYPRARCEVLEDI 575
              KLLCHL EELL+PY++  +RP+ G       W  +VE+LC+KV+R PRA+CE+LEDI
Sbjct: 541 --RKLLCHLVEELLEPYLE--VRPYRGAASPGEVWADVVEKLCEKVKRLPRAKCEILEDI 600

BLAST of MS000364 vs. TAIR 10
Match: AT5G62170.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G51850.1); Has 381 Blast hits to 359 proteins in 81 species: Archae - 0; Bacteria - 16; Metazoa - 101; Fungi - 21; Plants - 99; Viruses - 3; Other Eukaryotes - 141 (source: NCBI BLink). )

HSP 1 Score: 169.5 bits (428), Expect = 8.0e-42
Identity = 208/688 (30.23%), Postives = 308/688 (44.77%), Query Frame = 0

Query: 1   MGKQRYWSGHG--RATRRLKNEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPL 60
           MG+   W G G  +++ + K E          S   +   ++T+AGCM AVF +FDF  L
Sbjct: 1   MGRDWSWLGGGKKKSSSKSKEEDIKPTQPPPPSLAGN---TATAAGCMSAVFNIFDFQHL 60

Query: 61  N--QSHHHPPQSSLSVSLKPISLPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDG-LHI 120
               +HHH                   H+P G +APRNSLES EE  S    +KDG L+I
Sbjct: 61  QFPINHHH------------------LHLPKGVDAPRNSLESTEEETSFSPTRKDGNLNI 120

Query: 121 PEGI-VQIKTRSRSKDSSNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTT 180
             GI ++ K ++RS  +S +  + +   SPS KTPTLVARLMGLDL+P++   S TP ++
Sbjct: 121 SMGIKIKTKPQARSSSASLTPTETY---SPSIKTPTLVARLMGLDLVPDNYRSSPTPSSS 180

Query: 181 SSSVSPIYPLLQKPKYSK------------TGVSRSLPETPRTSCERKSNADQNY-HHRL 240
           SSS         +  ++K             G +RSLPETPR S  R+S     Y H R 
Sbjct: 181 SSSTLIDLKTPTRSSHAKKHRHYSLQRNSVDGGTRSLPETPRISLGRRSVDVNCYEHQRS 240

Query: 241 SLQIP-------------------------NYDKENSTSPSHYAKEIVKQIKESVSRKSG 300
           SL +                          + DKEN  SP  YA++IV Q+KE+VSR+  
Sbjct: 241 SLHLRDNNINVFPERESGINNVRLTRVKEIHEDKEN-RSPREYARQIVMQLKENVSRRRR 300

Query: 301 L-TDITN-NYQRRD---------------HDVMNQP----------KPKKISSSSSLSPK 360
           + TDITN   Q R+               HDV + P          KP  + +++  S  
Sbjct: 301 MGTDITNKETQPREVHESKKASSKTTIITHDVSSSPRLGLTEVPKTKPTSLQTNNVASKI 360

Query: 361 LRVLEAKNTNLKKLP--------SPKVEAIKGSKKAPAPAKKGNK--------QEEAFVV 420
           L     K  +  +LP        + K +  K +KK   P    ++        QEE FV 
Sbjct: 361 LETTAMKVQDKTRLPTVHEEPQGTEKEKQRKSTKKCKKPENFKSRLVKPPQSMQEEPFVR 420

Query: 421 PSRMTKA---------AIDSPRKKSKKTPLS-NELLSFSSVPTVVKKKEAKPPPMQSGAC 480
              +  +          I   +  SKKTPLS N L++F+SVPT +KKK+++P    S   
Sbjct: 421 SPAINNSNNNNNGHLLLIQGDKSSSKKTPLSINHLINFTSVPT-IKKKDSQPHHKSSNLK 480

Query: 481 NRNEGAETVTGDKESKPVLQLSTFHN-----NHKTLHHQHVIQLPNNNKHHIISAA---A 540
            R              P     + H+       +  +    ++    ++   IS A   +
Sbjct: 481 LRETQTPRNRASSSELPSFPSQSQHHIAPIAGGELEYITRTLRRTGIDRDTPISYAKWFS 540

Query: 541 DRHDITAEVDCVRK---ILTTRTNNFLENNTTQKEVPIWHPHYDTKLLCHLAEE----LL 568
             H +   +    +   + +TR  N  EN + +          + KLL HL +E    +L
Sbjct: 541 PSHPLDPSIFYFLEHFAVTSTRPRNSPENLSLR---------CNRKLLFHLVDEILADIL 600

BLAST of MS000364 vs. TAIR 10
Match: AT5G51850.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G62170.1); Has 384 Blast hits to 375 proteins in 79 species: Archae - 0; Bacteria - 14; Metazoa - 135; Fungi - 31; Plants - 92; Viruses - 0; Other Eukaryotes - 112 (source: NCBI BLink). )

HSP 1 Score: 113.6 bits (283), Expect = 5.2e-25
Identity = 176/626 (28.12%), Postives = 271/626 (43.29%), Query Frame = 0

Query: 19  NEGPHSNSNSSSSSTDHEEASSTSAGCMCAVFQLFDFHPLNQSHHHPPQSSLSVSLKPIS 78
           ++G  S       +     +  T+ GCM A + LFD      SHHH    S S S K + 
Sbjct: 9   DDGHGSEGGGRGGAFSSSRSKKTANGCMAAFYHLFD------SHHHLTIDSPSRS-KGLK 68

Query: 79  LPPDHHIPTGTEAPRNSLESDEELPSPCTKQKDGLHIPEGIVQIKTRSRSKDS------- 138
           L                   +E LPS   K K+  +IP G +++KT + +K S       
Sbjct: 69  L------------------MEESLPSTTYKDKEISNIPVG-MRVKTDTGTKSSRLRALVT 128

Query: 139 SNSDGDNFSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSSVSPIYPL----LQK 198
            +S   +   NSP +KTP LVARLMGLDLLP+ +         + S+S ++ +    +  
Sbjct: 129 DSSTSSSEICNSPGSKTPNLVARLMGLDLLPDKTD-------LNHSLSDLHTMSSHHITS 188

Query: 199 PKYSKTGVSRSLPETPRTSCERKSNADQNYHHRLSLQIP----------NYDKENSTSPS 258
            + SK G +RSLP +PR S  RKS+ D    HRLSLQ+             D+E S SP 
Sbjct: 189 HRLSKKG-TRSLPVSPRISSARKSDFD---IHRLSLQLNREKEFGRSRLKEDQEESHSPR 248

Query: 259 HYAKEIVKQIKES-VSRKSGLTDITNNYQ----------RRDHDVMNQPKPK-------- 318
            YA++IVKQIKE  V+R+    DITN+ +          RRD  V   P+ +        
Sbjct: 249 DYARQIVKQIKERVVTRRVVGMDITNSVKNREARPSHELRRDTTVSCSPRTRFSEKENKQ 308

Query: 319 ----KISSSSSLSPKLRVLEAKNT--------NLKKLPSPKVEAIKGSKKA--------- 378
               K +SSSS  P+  + + K T        +  ++   +++ I   KKA         
Sbjct: 309 STSHKPNSSSSSRPEPIIQKPKPTPVILGEKQSQNRVKQRQLKPINLCKKAETETRRPIK 368

Query: 379 PAPAKK-GNKQEEAFVVPSRMTKAAIDSPRKKSKKTPLSNELLSFSSVPTVVKKKEAKPP 438
           P+P     N++ E F+  SR  KA      KK KK P SN+L + S+          +PP
Sbjct: 369 PSPTSDIRNRKRETFLSDSRDVKAKPLHKIKKFKKIPKSNDLENISA---------TRPP 428

Query: 439 PMQSGACNRNEGAETVTGDKESKPVLQLSTFHNNHKTLHHQHVIQLPNNNKHHIISAAAD 498
             Q      NE    ++ +  S   ++ S+ H   K          P   ++H    AA 
Sbjct: 429 HQQI-----NERERLISNEAAS---IRSSSMHKTEK--------NSPQVARNHKFDDAAT 488

Query: 499 RHDITAEVDCVRKILTTRTNNFLENNTTQKEVPIWH--PHY------------DTKLLCH 558
             +I +E D + +I+         ++    ++ I+    H+            + +LL  
Sbjct: 489 --EINSEQDYIIRIMNLA--GIKSDSQAMLDLSIFRKLEHFGDYPSGTLALGCNRRLLFD 548

Query: 559 LAEELLKPYVDTMMRPWAGGGWPALVEELCDKVRRYPRARCEVLEDIDGIIEKDMDRLGM 569
           L  E+L   ++T+ +         L+ ELC  V RY      V E+I  +  K +     
Sbjct: 549 LVNEIL---IETVAKRRGNYQGSELISELCSAVARYSTKCYPVPEEIALVDVKRLLEKKK 565

BLAST of MS000364 vs. TAIR 10
Match: AT4G25430.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G51850.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 72.0 bits (175), Expect = 1.7e-12
Identity = 104/383 (27.15%), Postives = 170/383 (44.39%), Query Frame = 0

Query: 25  NSNSSSSSTDHEEASSTSAGCMCAVFQLFDFH----PLNQSHHHPPQSSLSVSLKPISLP 84
           N   S+ S+  ++ S+ + GC+ A++  F FH    P    HHH P           S+ 
Sbjct: 7   NGGRSTCSSKSKKNSNEANGCVTALYHFFHFHHFYFPSRHHHHHQP-----------SID 66

Query: 85  PDHHIPTGTEAPRNSLESDEELP--SPCTKQKDGLHIPEGIVQIKTRSRSKDSSNSDGDN 144
                  G  APRNSL+  EE P  +    +++GL+I  G  +   R    D+      +
Sbjct: 67  SPSRTRKGLVAPRNSLDLSEESPLSTNYKLEREGLNISVGGKKSTLRGLLVDT-----PS 126

Query: 145 FSGNSPSTKTPTLVARLMGLDLLPESSSPSTTPRTTSSSVSPIYPLLQKPKYSKTGV-SR 204
            + N P TKTP +VARLMGLDLLP++   + +PR            ++  + S  G  +R
Sbjct: 127 HNCNLPRTKTPNVVARLMGLDLLPDNLELTRSPRNG----------VRGHRLSGNGSGTR 186

Query: 205 SLPETPRTSCERKS-------NADQNYHH---RLSLQIPNYDKENSTSPSHYAKEIVKQI 264
           SLP +PR S + ++       N + N H    R  L+    D E S SP +  ++IVKQ 
Sbjct: 187 SLPASPRISSDSENHRLSLELNRENNKHEEFVRTRLKELKQD-EQSPSPRYSGRQIVKQT 246

Query: 265 KESVSRKSGLTDITN--NYQRRDHDVMNQPKPKKISSSSSLSPKLRVLEAKNT--NLKKL 324
           K+ V+ +    D+TN    +R      N+   K+ ++S++ +  LR  +   T   L K 
Sbjct: 247 KKRVTTRKFGMDVTNLLEKKRAGGAAQNRISQKEKTTSTNPAFVLRQYQQPATVITLSKE 306

Query: 325 PSPKVEAIKG-------SKKAPAPAKKGNKQEEAFVVP----SRMTKAAIDSPRKKSKKT 376
               +  I G       SK +P P      ++   + P    SR  +  +   +K+ KK 
Sbjct: 307 NQQSLRPISGWEKAESKSKFSPHPTPNNRNKQRKVLTPVSTHSRSNRCDL-LEKKQCKKI 361