MS000353 (gene) Bitter gourd (TR) v1

Overview
NameMS000353
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Description3-ketoacyl-CoA synthase
Locationscaffold44: 1088296 .. 1089753 (+)
RNA-Seq ExpressionMS000353
SyntenyMS000353
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCTGCTCCCAAGGTCATCCATGGCGGCACTCTCCTCCATTCCACTCAATTACTCAAACCCTTTCGCTTCACTTTCTTTCAACTTCAAATCCTTTTCGTTCTCCTAACTTCCATTGAAGCCTATCTTTTCTTACAAGGATGGAATCCCATTTTCCATATCTTCCCGCTGTCTTCTCTTGTCGTCTTCATCATAATCATCCACCACCCTTCCATCTTGAATATTGCTCCTCGTTCAGTCTATTTAGTTGACTTCTCATGCCTCAAACCGCCTAGTTTCTTCAGGGTTCCCTTCTCTACTTTCCTTGAAAATGCTACTTTGATGGATATTTTTGACAGTGAGAGCCTCTGTTTCATGGCCAAAACTCTTAAATCCTCTGGACAAAGTGAAGAAACATGCCTCCCTCCAGCCCTGCACTTCATTCCACCCAAAACCCATCAACAGGAATCCATTAATGAAGCCCATATGGTTCTGTTTCCTGTCATGGACGATCTTTTGACCAAAACCCATCTCTCTCCTCGTGACATTGATATTCTGATCGTCAATTGCAGTGGCTTTTGTCCTTCTCCTTCTCTATCCTCCATAGTTATCAACAAATATTCCATGAGAAGTGATATTAAGAGCTTCAATCTCTCCGGAATGGGGTGCAGTGCCAGTGCTGTTGCCATTCATTTAGCTGAAAGTCTCCTTCAAGTTCACAAAAATTCAAATGCCGTTGTACTCAGTACAGAAATTTTGTCGAACGGTTGGTATGCAGGAAAGGAACGCTCAAAATTGATCCTTAATTGCCTCTTCCGAATGGGCAGCGCAGCCATTCTGCTCACCAATAGAAAAGAGGCGAAGGATTCTTCAAAATATAAACTATTTAAAACGGTTCGAACACAGAGAGCTTTCGACGATAAAAGTTACCTTTCAGCCATACGCGAAGAGGACATGGACGGAAAGCTCGGCGTCACTCTCACAAGGGATTTACTCCAGGTCGCCGGAGAAACTCTCCGGTCAAACATAACCATTCTGGGTGATTCAGTCTTGCCGTTGTCGGAGAAACTACGGCACGGCGTTTCGAGGCTCGGAAAGCGATTTCTGGACAAATCGCAAGAAATCTACGTGCCCAATTTCAAGAGTGTGATTCAGCATTTCTGCTTGCCGGTATCGGGAGGTCCGGTGATACGGGAGATCGGAAAAATGCTGAAGCTTAAGGACAAGGACATGGAAGCGGCATTGATGACTCTGCATAGATTTGGGAACCAGTCGTCTTCTTCGCTGTGGTATGAACTCGCGTATTTGGAAGATCAAGAAAGAATTGGAAAAGGGGACAGAGTCTGGCAATTAGGGATGGGCACTGGGCCCAAATGTGTTAGTTTGATTTGGGAGTCTATACGGCCCATTGTTGGAGAGTCCAACAATGATCCATGGGCCGAAGTAATCCAGAATCCAATTTCAGGCCCACCAACC

mRNA sequence

ATGGAATCTGCTCCCAAGGTCATCCATGGCGGCACTCTCCTCCATTCCACTCAATTACTCAAACCCTTTCGCTTCACTTTCTTTCAACTTCAAATCCTTTTCGTTCTCCTAACTTCCATTGAAGCCTATCTTTTCTTACAAGGATGGAATCCCATTTTCCATATCTTCCCGCTGTCTTCTCTTGTCGTCTTCATCATAATCATCCACCACCCTTCCATCTTGAATATTGCTCCTCGTTCAGTCTATTTAGTTGACTTCTCATGCCTCAAACCGCCTAGTTTCTTCAGGGTTCCCTTCTCTACTTTCCTTGAAAATGCTACTTTGATGGATATTTTTGACAGTGAGAGCCTCTGTTTCATGGCCAAAACTCTTAAATCCTCTGGACAAAGTGAAGAAACATGCCTCCCTCCAGCCCTGCACTTCATTCCACCCAAAACCCATCAACAGGAATCCATTAATGAAGCCCATATGGTTCTGTTTCCTGTCATGGACGATCTTTTGACCAAAACCCATCTCTCTCCTCGTGACATTGATATTCTGATCGTCAATTGCAGTGGCTTTTGTCCTTCTCCTTCTCTATCCTCCATAGTTATCAACAAATATTCCATGAGAAGTGATATTAAGAGCTTCAATCTCTCCGGAATGGGGTGCAGTGCCAGTGCTGTTGCCATTCATTTAGCTGAAAGTCTCCTTCAAGTTCACAAAAATTCAAATGCCGTTGTACTCAGTACAGAAATTTTGTCGAACGGTTGGTATGCAGGAAAGGAACGCTCAAAATTGATCCTTAATTGCCTCTTCCGAATGGGCAGCGCAGCCATTCTGCTCACCAATAGAAAAGAGGCGAAGGATTCTTCAAAATATAAACTATTTAAAACGGTTCGAACACAGAGAGCTTTCGACGATAAAAGTTACCTTTCAGCCATACGCGAAGAGGACATGGACGGAAAGCTCGGCGTCACTCTCACAAGGGATTTACTCCAGGTCGCCGGAGAAACTCTCCGGTCAAACATAACCATTCTGGGTGATTCAGTCTTGCCGTTGTCGGAGAAACTACGGCACGGCGTTTCGAGGCTCGGAAAGCGATTTCTGGACAAATCGCAAGAAATCTACGTGCCCAATTTCAAGAGTGTGATTCAGCATTTCTGCTTGCCGGTATCGGGAGGTCCGGTGATACGGGAGATCGGAAAAATGCTGAAGCTTAAGGACAAGGACATGGAAGCGGCATTGATGACTCTGCATAGATTTGGGAACCAGTCGTCTTCTTCGCTGTGGTATGAACTCGCGTATTTGGAAGATCAAGAAAGAATTGGAAAAGGGGACAGAGTCTGGCAATTAGGGATGGGCACTGGGCCCAAATGTGTTAGTTTGATTTGGGAGTCTATACGGCCCATTGTTGGAGAGTCCAACAATGATCCATGGGCCGAAGTAATCCAGAATCCAATTTCAGGCCCACCAACC

Coding sequence (CDS)

ATGGAATCTGCTCCCAAGGTCATCCATGGCGGCACTCTCCTCCATTCCACTCAATTACTCAAACCCTTTCGCTTCACTTTCTTTCAACTTCAAATCCTTTTCGTTCTCCTAACTTCCATTGAAGCCTATCTTTTCTTACAAGGATGGAATCCCATTTTCCATATCTTCCCGCTGTCTTCTCTTGTCGTCTTCATCATAATCATCCACCACCCTTCCATCTTGAATATTGCTCCTCGTTCAGTCTATTTAGTTGACTTCTCATGCCTCAAACCGCCTAGTTTCTTCAGGGTTCCCTTCTCTACTTTCCTTGAAAATGCTACTTTGATGGATATTTTTGACAGTGAGAGCCTCTGTTTCATGGCCAAAACTCTTAAATCCTCTGGACAAAGTGAAGAAACATGCCTCCCTCCAGCCCTGCACTTCATTCCACCCAAAACCCATCAACAGGAATCCATTAATGAAGCCCATATGGTTCTGTTTCCTGTCATGGACGATCTTTTGACCAAAACCCATCTCTCTCCTCGTGACATTGATATTCTGATCGTCAATTGCAGTGGCTTTTGTCCTTCTCCTTCTCTATCCTCCATAGTTATCAACAAATATTCCATGAGAAGTGATATTAAGAGCTTCAATCTCTCCGGAATGGGGTGCAGTGCCAGTGCTGTTGCCATTCATTTAGCTGAAAGTCTCCTTCAAGTTCACAAAAATTCAAATGCCGTTGTACTCAGTACAGAAATTTTGTCGAACGGTTGGTATGCAGGAAAGGAACGCTCAAAATTGATCCTTAATTGCCTCTTCCGAATGGGCAGCGCAGCCATTCTGCTCACCAATAGAAAAGAGGCGAAGGATTCTTCAAAATATAAACTATTTAAAACGGTTCGAACACAGAGAGCTTTCGACGATAAAAGTTACCTTTCAGCCATACGCGAAGAGGACATGGACGGAAAGCTCGGCGTCACTCTCACAAGGGATTTACTCCAGGTCGCCGGAGAAACTCTCCGGTCAAACATAACCATTCTGGGTGATTCAGTCTTGCCGTTGTCGGAGAAACTACGGCACGGCGTTTCGAGGCTCGGAAAGCGATTTCTGGACAAATCGCAAGAAATCTACGTGCCCAATTTCAAGAGTGTGATTCAGCATTTCTGCTTGCCGGTATCGGGAGGTCCGGTGATACGGGAGATCGGAAAAATGCTGAAGCTTAAGGACAAGGACATGGAAGCGGCATTGATGACTCTGCATAGATTTGGGAACCAGTCGTCTTCTTCGCTGTGGTATGAACTCGCGTATTTGGAAGATCAAGAAAGAATTGGAAAAGGGGACAGAGTCTGGCAATTAGGGATGGGCACTGGGCCCAAATGTGTTAGTTTGATTTGGGAGTCTATACGGCCCATTGTTGGAGAGTCCAACAATGATCCATGGGCCGAAGTAATCCAGAATCCAATTTCAGGCCCACCAACC

Protein sequence

MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFVLLTSIEAYLFLQGWNPIFHIFPLSSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQNPISGPPT
Homology
BLAST of MS000353 vs. NCBI nr
Match: XP_022140890.1 (3-ketoacyl-CoA synthase 5-like [Momordica charantia])

HSP 1 Score: 951.4 bits (2458), Expect = 2.9e-273
Identity = 479/486 (98.56%), Postives = 483/486 (99.38%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFVLLTSIEAYLFLQGWNPIFHIFPLSS 60
           MESAP+VIHGGTLLHSTQLLKP RFTFFQLQILFVLLTSIEAYLFL+GWNPIFHIFPLSS
Sbjct: 1   MESAPEVIHGGTLLHSTQLLKPLRFTFFQLQILFVLLTSIEAYLFLEGWNPIFHIFPLSS 60

Query: 61  LVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFM 120
           LVVFIIII+HPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFM
Sbjct: 61  LVVFIIIINHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFM 120

Query: 121 AKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDIL 180
           AKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDIL
Sbjct: 121 AKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDIL 180

Query: 181 IVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAV 240
           IVNCSGFCPSP LSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAV
Sbjct: 181 IVNCSGFCPSPCLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAV 240

Query: 241 VLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFD 300
           VLSTEILSNGWYAGKERSKLILNCLFRMGS AILLTNRKEAKDSSKYKLFKTVRTQRAFD
Sbjct: 241 VLSTEILSNGWYAGKERSKLILNCLFRMGSGAILLTNRKEAKDSSKYKLFKTVRTQRAFD 300

Query: 301 DKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGK 360
           DKSYLSAIRE+DMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGK
Sbjct: 301 DKSYLSAIREDDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGK 360

Query: 361 RFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSS 420
           RFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSS
Sbjct: 361 RFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSS 420

Query: 421 SSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQNP 480
           SSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQNP
Sbjct: 421 SSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQNP 480

Query: 481 ISGPPT 487
           ISGPPT
Sbjct: 481 ISGPPT 486

BLAST of MS000353 vs. NCBI nr
Match: XP_022140855.1 (3-ketoacyl-CoA synthase 5-like [Momordica charantia])

HSP 1 Score: 778.5 bits (2009), Expect = 3.4e-221
Identity = 391/479 (81.63%), Postives = 432/479 (90.19%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQ--ILFVLLTSIEAYLFLQGWNPIFHIFPL 60
           M+ APKV HGGT+LHSTQL    R TFFQL   ++ +L++SIE Y+FL+GWNPIFHIF L
Sbjct: 1   MDYAPKVSHGGTVLHSTQLFTHLRLTFFQLLNFLVLILVSSIEIYIFLKGWNPIFHIFLL 60

Query: 61  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 120
            S  V  III  PSILN APRSVYLVDFSCLKPPSF R+PFS FLENATL+D FDS+S+ 
Sbjct: 61  LSCYVVFIII-KPSILN-APRSVYLVDFSCLKPPSFCRIPFSFFLENATLIDTFDSDSIS 120

Query: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 180
           FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINE HMVLFPVM+DLLTKTHLSPRDID
Sbjct: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEVHMVLFPVMEDLLTKTHLSPRDID 180

Query: 181 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 240
           ILIVNCSGFCPSPSLSSIVIN++SMRSDIKS+NLSGMGCSASAVAI LAE+LLQVH+NS 
Sbjct: 181 ILIVNCSGFCPSPSLSSIVINRFSMRSDIKSYNLSGMGCSASAVAIQLAENLLQVHENSY 240

Query: 241 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 300
           A+VLSTEILSNGWYAGKE SKL+LNC FRMGSAAILLTNRKEAKDSSKYKL KT+R Q +
Sbjct: 241 AIVLSTEILSNGWYAGKEHSKLLLNCFFRMGSAAILLTNRKEAKDSSKYKLLKTLRNQTS 300

Query: 301 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 360
           FDDKSYLSAIREED DGKLGV++TRD LQV  ETLR+NITI+G S+LPLSEKLRHGVSRL
Sbjct: 301 FDDKSYLSAIREEDADGKLGVSVTRDTLQVFPETLRTNITIMGSSILPLSEKLRHGVSRL 360

Query: 361 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 420
            KR++DKSQEIYVPNFKSVI+HFCLPVSGGPVIR IGK+LKLK+KD+EA LMTLHRFGNQ
Sbjct: 361 RKRYVDKSQEIYVPNFKSVIRHFCLPVSGGPVIRAIGKVLKLKEKDLEAGLMTLHRFGNQ 420

Query: 421 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           SSS+LWYELAYLE +ER+ KGD VWQ+GMGTGPKCVSLIWE +RPI+GES NDPWA+ I
Sbjct: 421 SSSALWYELAYLEAKERVEKGDTVWQIGMGTGPKCVSLIWECVRPIIGESKNDPWAQAI 477

BLAST of MS000353 vs. NCBI nr
Match: XP_023542466.1 (3-ketoacyl-CoA synthase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.5 bits (1970), Expect = 1.1e-216
Identity = 390/489 (79.75%), Postives = 428/489 (87.53%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFV--LLTSIEAYLFLQGWNPIFHIFPL 60
           MESAPKV HGG  L S QL+   R TFFQLQ  F+  LL SIE ++FL+GWNP+FHIF L
Sbjct: 1   MESAPKVSHGGIALQSAQLMTRLRLTFFQLQQFFILTLLISIECHVFLEGWNPMFHIFFL 60

Query: 61  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 120
            S  V  + +     LN APRSVYLVDFSCLKPPSF RVPF+TFLENATLMD+FDSESL 
Sbjct: 61  FSCFVVYMTLRR-FFLN-APRSVYLVDFSCLKPPSFLRVPFATFLENATLMDVFDSESLS 120

Query: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 180
           FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINE HMVLFPVMDDLLTKTHLSP DID
Sbjct: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEVHMVLFPVMDDLLTKTHLSPHDID 180

Query: 181 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 240
           ILIVNCSGFCPSPSLSSI+IN+YSMRSDIKSFNLSGMGCSASAVAIHLAE+LLQVH+NSN
Sbjct: 181 ILIVNCSGFCPSPSLSSIIINRYSMRSDIKSFNLSGMGCSASAVAIHLAENLLQVHENSN 240

Query: 241 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 300
           AVVLSTEILSNGWYAGKE S+LILNC FRMG AAILL+NRKEAK+ SKYKLFK++RT  +
Sbjct: 241 AVVLSTEILSNGWYAGKEPSRLILNCYFRMGGAAILLSNRKEAKNYSKYKLFKSLRTLTS 300

Query: 301 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 360
           FDDKSYLSA+REED DGKLGV++TRD+LQV  ETLR NIT+LG S+LPLSEKL+H VSRL
Sbjct: 301 FDDKSYLSAMREEDKDGKLGVSVTRDMLQVIPETLRINITLLGSSILPLSEKLKHVVSRL 360

Query: 361 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 420
            KRFL KSQEIY+PNFK+ I HFCLPVSGG VIR IGK+LKL DKD+EAALMTLHRFGNQ
Sbjct: 361 RKRFLYKSQEIYIPNFKTEINHFCLPVSGGAVIRAIGKVLKLNDKDLEAALMTLHRFGNQ 420

Query: 421 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQ 480
           SSS+LWYELAY E +ER+ KGD+VWQ+GMGTGPKCVSLIWE IRPI GES  DPWA+VI 
Sbjct: 421 SSSALWYELAYFEAKERVEKGDKVWQIGMGTGPKCVSLIWECIRPISGESKKDPWAQVIH 480

Query: 481 N-PISGPPT 487
           + PISGP T
Sbjct: 481 HYPISGPST 487

BLAST of MS000353 vs. NCBI nr
Match: XP_022968007.1 (3-ketoacyl-CoA synthase 7-like [Cucurbita maxima])

HSP 1 Score: 762.7 bits (1968), Expect = 1.9e-216
Identity = 393/489 (80.37%), Postives = 425/489 (86.91%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFVLLTSI--EAYLFLQGWNPIFHIFPL 60
           MESAPK  HG  +LHS +LL   R T FQLQ  FVL+  I  E Y+FL+GWNPI HIF L
Sbjct: 1   MESAPKFSHGDNVLHSKELLAHLRGTLFQLQTFFVLILIIFVETYIFLKGWNPICHIFLL 60

Query: 61  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 120
            S  V  III H SIL  APR VYLVDFSCL+PPSF R+PFS FLENATLM IFDSESL 
Sbjct: 61  LSCYVVFIIIKH-SILK-APRPVYLVDFSCLRPPSFCRIPFSFFLENATLMGIFDSESLI 120

Query: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 180
           FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINE HMVLFPVMDDLLTKTHLSP DID
Sbjct: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEVHMVLFPVMDDLLTKTHLSPPDID 180

Query: 181 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 240
           ILIVNCSGFCPSPSLSSI+IN+YSMRSDIKSFNLSGMGCSASAVAIHLAE+LLQVH+NSN
Sbjct: 181 ILIVNCSGFCPSPSLSSIIINRYSMRSDIKSFNLSGMGCSASAVAIHLAENLLQVHENSN 240

Query: 241 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 300
           AVVLSTEILSNGWYAGKE S+LILNC FRMG AAILLTNRKEAKD SKYKLFK++RT  +
Sbjct: 241 AVVLSTEILSNGWYAGKEPSRLILNCYFRMGGAAILLTNRKEAKDYSKYKLFKSLRTLTS 300

Query: 301 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 360
           FDDKSYLSA+REED DGKLGV++TRD LQV  ETLR NIT+LG S+LPLSEKL+H +SRL
Sbjct: 301 FDDKSYLSAMREEDKDGKLGVSVTRDTLQVFPETLRINITLLGSSILPLSEKLKHVISRL 360

Query: 361 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 420
            KRFLDKSQEIY+PNFK+VI H CLPVSGG VIR IGK+LKL DKD+EAALMTLHRFGNQ
Sbjct: 361 RKRFLDKSQEIYIPNFKTVINHICLPVSGGAVIRAIGKVLKLNDKDLEAALMTLHRFGNQ 420

Query: 421 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI- 480
           SSS+LWYELAY E +ER+ KGD+VWQ+GMGTGPKCVSLIWE IRPI GES  DPWA+VI 
Sbjct: 421 SSSALWYELAYFEAKERVKKGDKVWQIGMGTGPKCVSLIWECIRPISGESKKDPWAQVIH 480

Query: 481 QNPISGPPT 487
           Q PISGP T
Sbjct: 481 QYPISGPST 487

BLAST of MS000353 vs. NCBI nr
Match: XP_022968008.1 (3-ketoacyl-CoA synthase 7-like [Cucurbita maxima])

HSP 1 Score: 762.7 bits (1968), Expect = 1.9e-216
Identity = 389/489 (79.55%), Postives = 428/489 (87.53%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFV--LLTSIEAYLFLQGWNPIFHIFPL 60
           MESAPKV HGG  L S +L+     TFFQLQ  F+  L+ SIE+++FL+GWNP+FH+F L
Sbjct: 1   MESAPKVSHGGIALQSARLMTHLSLTFFQLQHFFILTLVISIESHIFLKGWNPMFHLFFL 60

Query: 61  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 120
            S  V  +I+     LN APRSVYLVDFSCLKPPSF RVPF+TFLENATLM IFDSESL 
Sbjct: 61  FSCFVIYMILRR-FFLN-APRSVYLVDFSCLKPPSFLRVPFATFLENATLMGIFDSESLN 120

Query: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 180
           FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINE HMVLFPVMDDLLTKTHLSP DID
Sbjct: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEVHMVLFPVMDDLLTKTHLSPHDID 180

Query: 181 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 240
           ILIVNCSGFCPSPSLSSI+IN+YSMRSDIKSFNLSGMGCSASAVAIHLAE+LLQVH+NSN
Sbjct: 181 ILIVNCSGFCPSPSLSSIIINRYSMRSDIKSFNLSGMGCSASAVAIHLAENLLQVHENSN 240

Query: 241 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 300
           AVVLSTEILSNGWYAGKE S+LILNC FRMG AAILLTNRKEAKD SKYKLFK++RT  +
Sbjct: 241 AVVLSTEILSNGWYAGKEPSRLILNCYFRMGGAAILLTNRKEAKDYSKYKLFKSLRTLTS 300

Query: 301 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 360
           FDDKSYLSA+REED DGKLGV++TRD LQV  ETLR NIT+LG S+LPLSEKL+H +SRL
Sbjct: 301 FDDKSYLSAMREEDKDGKLGVSVTRDTLQVFPETLRINITLLGSSILPLSEKLKHVISRL 360

Query: 361 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 420
            KRFLDKSQEIY+PNFK+VI H CLPVSGG VIR IGK+LKL DKD+EAALMTLHRFGNQ
Sbjct: 361 RKRFLDKSQEIYIPNFKTVINHICLPVSGGAVIRAIGKVLKLNDKDLEAALMTLHRFGNQ 420

Query: 421 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQ 480
           SSS+LWYELAY E +ER+ KGD+VW +GMGTGPKCVSLIWE IRPI GES NDPWA+VI 
Sbjct: 421 SSSALWYELAYFEAKERVKKGDKVWHIGMGTGPKCVSLIWECIRPISGESKNDPWAQVIH 480

Query: 481 N-PISGPPT 487
           + PISGP T
Sbjct: 481 HYPISGPST 487

BLAST of MS000353 vs. ExPASy Swiss-Prot
Match: Q9C6L5 (3-ketoacyl-CoA synthase 5 OS=Arabidopsis thaliana OX=3702 GN=KCS5 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.1e-117
Identity = 211/427 (49.41%), Postives = 299/427 (70.02%), Query Frame = 0

Query: 52  IFHIFPLSSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDI 111
           + HI   S L++F+  ++  S     PR+VYLVD+SC KPP   RVPFS+F+E++ L+  
Sbjct: 59  LLHILCSSFLIIFVSTVYFMS----KPRTVYLVDYSCYKPPVTCRVPFSSFMEHSRLILK 118

Query: 112 FDSESLCFMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTH 171
            + +S+ F  + L+ SG  EETCLPPA+H+IPP    + + NEA MV+F  M+DL   T 
Sbjct: 119 DNPKSVEFQMRILERSGLGEETCLPPAIHYIPPTPTMESARNEAQMVIFTAMEDLFKNTG 178

Query: 172 LSPRDIDILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLL 231
           L P+DIDILIVNCS F P+PSLS+++INKY +RS+IKS+NLSGMGCSAS +++ +A  LL
Sbjct: 179 LKPKDIDILIVNCSLFSPTPSLSAMIINKYKLRSNIKSYNLSGMGCSASLISVDVARDLL 238

Query: 232 QVHKNSNAVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFK 291
           QVH NSNA+++STEI++  +Y G ER+ L+ NCLFRMG AAILL+NR+  +  +KYKL  
Sbjct: 239 QVHPNSNAIIISTEIITPNYYKGNERAMLLPNCLFRMGGAAILLSNRRSDRWRAKYKLCH 298

Query: 292 TVRTQRAFDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKL 351
            VRT R  DDKSY   + +ED +G +G+ L++DL+ +AGE L++NIT +G  VLP SE+L
Sbjct: 299 LVRTHRGADDKSYNCVMEQEDKNGNVGINLSKDLMTIAGEALKANITTIGPLVLPASEQL 358

Query: 352 RHGVSRLGKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMT 411
               S +G++  +   + Y+P+FK   +HFC+   G  VI E+ K L+L  + +EA+ MT
Sbjct: 359 LFLSSLIGRKIFNPKWKPYIPDFKQAFEHFCIHAGGRAVIDELQKNLQLSGEHVEASRMT 418

Query: 412 LHRFGNQSSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNND 471
           LHRFGN SSSSLWYEL+Y+E Q R+ + DRVWQ+  G+G KC S +W+  R I   ++  
Sbjct: 419 LHRFGNTSSSSLWYELSYIEAQGRMKRNDRVWQIAFGSGFKCNSAVWKCNRTIKTPTDG- 478

Query: 472 PWAEVIQ 479
            W++ I+
Sbjct: 479 AWSDCIE 480

BLAST of MS000353 vs. ExPASy Swiss-Prot
Match: Q9XF43 (3-ketoacyl-CoA synthase 6 OS=Arabidopsis thaliana OX=3702 GN=CUT1 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 4.1e-116
Identity = 212/436 (48.62%), Postives = 297/436 (68.12%), Query Frame = 0

Query: 46  LQGWN----PIFHIFPLSSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFST 105
           L  WN     +  +   S  V+FI  ++  S     PR++YLVD+SC KPP   RVPF+T
Sbjct: 54  LNVWNSLQFDLVQVLCSSFFVIFISTVYFMS----KPRTIYLVDYSCYKPPVTCRVPFAT 113

Query: 106 FLENATLMDIFDSESLCFMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFP 165
           F+E++ L+     +S+ F  + L+ SG  EETCLPPA+H+IPP      + +EA MV+F 
Sbjct: 114 FMEHSRLILKDKPKSVEFQMRILERSGLGEETCLPPAIHYIPPTPTMDAARSEAQMVIFE 173

Query: 166 VMDDLLTKTHLSPRDIDILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASA 225
            MDDL  KT L P+D+DILIVNCS F P+PSLS++VINKY +RS+IKSFNLSGMGCSA  
Sbjct: 174 AMDDLFKKTGLKPKDVDILIVNCSLFSPTPSLSAMVINKYKLRSNIKSFNLSGMGCSAGL 233

Query: 226 VAIHLAESLLQVHKNSNAVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEA 285
           +++ LA  LLQVH NSNA+++STEI++  +Y G ER+ L+ NCLFRMG+AAI ++NR+  
Sbjct: 234 ISVDLARDLLQVHPNSNAIIVSTEIITPNYYQGNERAMLLPNCLFRMGAAAIHMSNRRSD 293

Query: 286 KDSSKYKLFKTVRTQRAFDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILG 345
           +  +KYKL   VRT R  DDKS+     +ED +G +G+ L++DL+ +AGE L++NIT +G
Sbjct: 294 RWRAKYKLSHLVRTHRGADDKSFYCVYEQEDKEGHVGINLSKDLMAIAGEALKANITTIG 353

Query: 346 DSVLPLSEKLRHGVSRLGKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLK 405
             VLP SE+L    S +G++  +   + Y+P+FK   +HFC+   G  VI E+ K L+L 
Sbjct: 354 PLVLPASEQLLFLTSLIGRKIFNPKWKPYIPDFKLAFEHFCIHAGGRAVIDELQKNLQLS 413

Query: 406 DKDMEAALMTLHRFGNQSSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESI 465
            + +EA+ MTLHRFGN SSSSLWYEL+Y+E + R+ +GDRVWQ+  G+G KC S +W+  
Sbjct: 414 GEHVEASRMTLHRFGNTSSSSLWYELSYIESKGRMRRGDRVWQIAFGSGFKCNSAVWKCN 473

Query: 466 RPIVGESNNDPWAEVI 478
           R I     + PW++ I
Sbjct: 474 RTI-KTPKDGPWSDCI 484

BLAST of MS000353 vs. ExPASy Swiss-Prot
Match: Q9LN49 (3-ketoacyl-CoA synthase 4 OS=Arabidopsis thaliana OX=3702 GN=KCS4 PE=2 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 8.2e-109
Identity = 192/419 (45.82%), Postives = 287/419 (68.50%), Query Frame = 0

Query: 59  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 118
           S+++VF + ++    +   PR VYLVDFSC  PP   + P++ F+E++ L   FD  +L 
Sbjct: 94  SAILVFGLTVY----VMTRPRPVYLVDFSCYLPPDHLKAPYARFMEHSRLTGDFDDSALE 153

Query: 119 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 178
           F  K L+ SG  E+T +P A+H++PP+     +  EA  V+F  +D+L   T++ P+DI 
Sbjct: 154 FQRKILERSGLGEDTYVPEAMHYVPPRISMAAAREEAEQVMFGALDNLFANTNVKPKDIG 213

Query: 179 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 238
           IL+VNCS F P+PSLS++++NKY +R +I+S+NL GMGCSA  +A+ LA+ +L VH+N+ 
Sbjct: 214 ILVVNCSLFNPTPSLSAMIVNKYKLRGNIRSYNLGGMGCSAGVIAVDLAKDMLLVHRNTY 273

Query: 239 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 298
           AVV+STE ++  WY G ++S LI NCLFR+G +A+LL+N+   K  SKY+L   VRT R 
Sbjct: 274 AVVVSTENITQNWYFGNKKSMLIPNCLFRVGGSAVLLSNKSRDKRRSKYRLVHVVRTHRG 333

Query: 299 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 358
            DDK++    +E+D  G+ GV+L++DL+ +AGETL++NIT LG  VLP+SE++   ++ +
Sbjct: 334 ADDKAFRCVYQEQDDTGRTGVSLSKDLMAIAGETLKTNITTLGPLVLPISEQILFFMTLV 393

Query: 359 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 418
            K+  +   + Y+P+FK   +HFC+   G  VI E+ K L+L    +EA+ MTLHRFGN 
Sbjct: 394 VKKLFNGKVKPYIPDFKLAFEHFCIHAGGRAVIDELEKNLQLSPVHVEASRMTLHRFGNT 453

Query: 419 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           SSSS+WYELAY+E + R+ +G+RVWQ+  G+G KC S IWE++R  V  SNN PW + I
Sbjct: 454 SSSSIWYELAYIEAKGRMRRGNRVWQIAFGSGFKCNSAIWEALRH-VKPSNNSPWEDCI 507

BLAST of MS000353 vs. ExPASy Swiss-Prot
Match: Q9SIX1 (3-ketoacyl-CoA synthase 9 OS=Arabidopsis thaliana OX=3702 GN=KCS9 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.6e-104
Identity = 203/462 (43.94%), Postives = 302/462 (65.37%), Query Frame = 0

Query: 25  FTFFQLQILFVLLTSI---------EAYLFLQGWNPIFHIFPLSSLVVFIIIIHHPSILN 84
           F    + ++ VL+T I         + +L LQ +N +  IF LS+L +F   ++  S   
Sbjct: 49  FKLCLVPLMAVLVTEISRLTTDDLYQIWLHLQ-YNLVAFIF-LSALAIFGSTVYIMS--- 108

Query: 85  IAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFMAKTLKSSGQSEETCL 144
             PRSVYLVD+SC  PP   +V +  F++++ L++ F+  SL F  K L+ SG  EET L
Sbjct: 109 -RPRSVYLVDYSCYLPPESLQVKYQKFMDHSKLIEDFNESSLEFQRKILERSGLGEETYL 168

Query: 145 PPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDILIVNCSGFCPSPSLSS 204
           P ALH IPP+     +  E+  V+F  +D L   T ++PRDI +L+VNCS F P+PSLS+
Sbjct: 169 PEALHCIPPRPTMMAAREESEQVMFGALDKLFENTKINPRDIGVLVVNCSLFNPTPSLSA 228

Query: 205 IVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAVVLSTEILSNGWYAGK 264
           +++NKY +R ++KSFNL GMGCSA  ++I LA+ +LQVH+N+ AVV+STE ++  WY G 
Sbjct: 229 MIVNKYKLRGNVKSFNLGGMGCSAGVISIDLAKDMLQVHRNTYAVVVSTENITQNWYFGN 288

Query: 265 ERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFDDKSYLSAIREEDMDG 324
           +++ LI NCLFR+G +AILL+N+ + +  SKYKL  TVRT +   +K++    +E+D +G
Sbjct: 289 KKAMLIPNCLFRVGGSAILLSNKGKDRRRSKYKLVHTVRTHKGAVEKAFNCVYQEQDDNG 348

Query: 325 KLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGKRFLDKSQEIYVPNFK 384
           K GV+L++DL+ +AGE L++NIT LG  VLP+SE++   ++ + K+  +   + Y+P+FK
Sbjct: 349 KTGVSLSKDLMAIAGEALKANITTLGPLVLPISEQILFFMTLVTKKLFNSKLKPYIPDFK 408

Query: 385 SVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSSSSLWYELAYLEDQER 444
               HFC+   G  VI E+ K L+L    +EA+ MTLHRFGN SSSS+WYELAY+E + R
Sbjct: 409 LAFDHFCIHAGGRAVIDELEKNLQLSQTHVEASRMTLHRFGNTSSSSIWYELAYIEAKGR 468

Query: 445 IGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           + KG+RVWQ+  G+G KC S +W ++   V  S + PW   I
Sbjct: 469 MKKGNRVWQIAFGSGFKCNSAVWVALNN-VKPSVSSPWEHCI 503

BLAST of MS000353 vs. ExPASy Swiss-Prot
Match: O65677 (3-ketoacyl-CoA synthase 17 OS=Arabidopsis thaliana OX=3702 GN=KCS17 PE=1 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 4.6e-104
Identity = 201/457 (43.98%), Postives = 295/457 (64.55%), Query Frame = 0

Query: 23  FRFTFFQLQILFVLLTSIEAYLFLQ-GWNPIFHIFPLSSLVVFIIIIHHPSILNIAPRSV 82
           F+  F  L  +  +  S+ +   LQ  +N    IF ++  +V  I+          PRS+
Sbjct: 28  FKLMFLPLMAVLFMNVSLLSLNHLQLYYNSTGFIFVITLAIVGSIV-----FFMSRPRSI 87

Query: 83  YLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFMAKTLKSSGQSEETCLPPALHF 142
           YL+D+SC  PPS  +V +  F+ N++L+  F   SL F  K L  SG  EET LP ++H 
Sbjct: 88  YLLDYSCYLPPSSQKVSYQKFMNNSSLIQDFSETSLEFQRKILIRSGLGEETYLPDSIHS 147

Query: 143 IPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDILIVNCSGFCPSPSLSSIVINKY 202
           IPP+     +  EA  V+F  +D+L   T ++PR+I +L+VNCS F P+PSLS++++NKY
Sbjct: 148 IPPRPTMAAAREEAEQVIFGALDNLFENTKINPREIGVLVVNCSLFNPTPSLSAMIVNKY 207

Query: 203 SMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAVVLSTEILSNGWYAGKERSKLI 262
            +R +IKSFNL GMGCSA  +A+ LA  +LQ+H+N+ A+V+STE ++  WY G +++ LI
Sbjct: 208 KLRGNIKSFNLGGMGCSAGVIAVDLASDMLQIHRNTFALVVSTENITQNWYFGNKKAMLI 267

Query: 263 LNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFDDKSYLSAIREEDMDGKLGVTL 322
            NCLFR+G +A+LL+N+   +  SKYKL  TVRT +  D+ ++    +E+D   K GV+L
Sbjct: 268 PNCLFRVGGSAVLLSNKPLDRKRSKYKLVHTVRTHKGSDENAFNCVYQEQDECLKTGVSL 327

Query: 323 TRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGKR-FLDKSQEIYVPNFKSVIQH 382
           ++DL+ +AGE L++NIT LG  VLP+SE++    + + KR F DK ++ Y+P+FK  + H
Sbjct: 328 SKDLMAIAGEALKTNITSLGPLVLPISEQILFFATFVAKRLFNDKKKKPYIPDFKLALDH 387

Query: 383 FCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSSSSLWYELAYLEDQERIGKGD 442
           FC+   G  VI E+ K LKL  K +EA+ MTLHRFGN SSSS+WYELAY E + R+ KG+
Sbjct: 388 FCIHAGGRAVIDELEKSLKLSPKHVEASRMTLHRFGNTSSSSIWYELAYTEAKGRMRKGN 447

Query: 443 RVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           RVWQ+  G+G KC S +W ++R  V  S N+PW   I
Sbjct: 448 RVWQIAFGSGFKCNSAVWVALRN-VEPSVNNPWEHCI 478

BLAST of MS000353 vs. ExPASy TrEMBL
Match: A0A6J1CGF6 (3-ketoacyl-CoA synthase OS=Momordica charantia OX=3673 GN=LOC111011446 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.4e-273
Identity = 479/486 (98.56%), Postives = 483/486 (99.38%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFVLLTSIEAYLFLQGWNPIFHIFPLSS 60
           MESAP+VIHGGTLLHSTQLLKP RFTFFQLQILFVLLTSIEAYLFL+GWNPIFHIFPLSS
Sbjct: 1   MESAPEVIHGGTLLHSTQLLKPLRFTFFQLQILFVLLTSIEAYLFLEGWNPIFHIFPLSS 60

Query: 61  LVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFM 120
           LVVFIIII+HPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFM
Sbjct: 61  LVVFIIIINHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFM 120

Query: 121 AKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDIL 180
           AKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDIL
Sbjct: 121 AKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDIL 180

Query: 181 IVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAV 240
           IVNCSGFCPSP LSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAV
Sbjct: 181 IVNCSGFCPSPCLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAV 240

Query: 241 VLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFD 300
           VLSTEILSNGWYAGKERSKLILNCLFRMGS AILLTNRKEAKDSSKYKLFKTVRTQRAFD
Sbjct: 241 VLSTEILSNGWYAGKERSKLILNCLFRMGSGAILLTNRKEAKDSSKYKLFKTVRTQRAFD 300

Query: 301 DKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGK 360
           DKSYLSAIRE+DMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGK
Sbjct: 301 DKSYLSAIREDDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGK 360

Query: 361 RFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSS 420
           RFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSS
Sbjct: 361 RFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSS 420

Query: 421 SSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQNP 480
           SSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQNP
Sbjct: 421 SSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQNP 480

Query: 481 ISGPPT 487
           ISGPPT
Sbjct: 481 ISGPPT 486

BLAST of MS000353 vs. ExPASy TrEMBL
Match: A0A6J1CGC2 (3-ketoacyl-CoA synthase OS=Momordica charantia OX=3673 GN=LOC111011424 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.7e-221
Identity = 391/479 (81.63%), Postives = 432/479 (90.19%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQ--ILFVLLTSIEAYLFLQGWNPIFHIFPL 60
           M+ APKV HGGT+LHSTQL    R TFFQL   ++ +L++SIE Y+FL+GWNPIFHIF L
Sbjct: 1   MDYAPKVSHGGTVLHSTQLFTHLRLTFFQLLNFLVLILVSSIEIYIFLKGWNPIFHIFLL 60

Query: 61  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 120
            S  V  III  PSILN APRSVYLVDFSCLKPPSF R+PFS FLENATL+D FDS+S+ 
Sbjct: 61  LSCYVVFIII-KPSILN-APRSVYLVDFSCLKPPSFCRIPFSFFLENATLIDTFDSDSIS 120

Query: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 180
           FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINE HMVLFPVM+DLLTKTHLSPRDID
Sbjct: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEVHMVLFPVMEDLLTKTHLSPRDID 180

Query: 181 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 240
           ILIVNCSGFCPSPSLSSIVIN++SMRSDIKS+NLSGMGCSASAVAI LAE+LLQVH+NS 
Sbjct: 181 ILIVNCSGFCPSPSLSSIVINRFSMRSDIKSYNLSGMGCSASAVAIQLAENLLQVHENSY 240

Query: 241 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 300
           A+VLSTEILSNGWYAGKE SKL+LNC FRMGSAAILLTNRKEAKDSSKYKL KT+R Q +
Sbjct: 241 AIVLSTEILSNGWYAGKEHSKLLLNCFFRMGSAAILLTNRKEAKDSSKYKLLKTLRNQTS 300

Query: 301 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 360
           FDDKSYLSAIREED DGKLGV++TRD LQV  ETLR+NITI+G S+LPLSEKLRHGVSRL
Sbjct: 301 FDDKSYLSAIREEDADGKLGVSVTRDTLQVFPETLRTNITIMGSSILPLSEKLRHGVSRL 360

Query: 361 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 420
            KR++DKSQEIYVPNFKSVI+HFCLPVSGGPVIR IGK+LKLK+KD+EA LMTLHRFGNQ
Sbjct: 361 RKRYVDKSQEIYVPNFKSVIRHFCLPVSGGPVIRAIGKVLKLKEKDLEAGLMTLHRFGNQ 420

Query: 421 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           SSS+LWYELAYLE +ER+ KGD VWQ+GMGTGPKCVSLIWE +RPI+GES NDPWA+ I
Sbjct: 421 SSSALWYELAYLEAKERVEKGDTVWQIGMGTGPKCVSLIWECVRPIIGESKNDPWAQAI 477

BLAST of MS000353 vs. ExPASy TrEMBL
Match: A0A6J1HSB5 (3-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111467382 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 9.4e-217
Identity = 393/489 (80.37%), Postives = 425/489 (86.91%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFVLLTSI--EAYLFLQGWNPIFHIFPL 60
           MESAPK  HG  +LHS +LL   R T FQLQ  FVL+  I  E Y+FL+GWNPI HIF L
Sbjct: 1   MESAPKFSHGDNVLHSKELLAHLRGTLFQLQTFFVLILIIFVETYIFLKGWNPICHIFLL 60

Query: 61  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 120
            S  V  III H SIL  APR VYLVDFSCL+PPSF R+PFS FLENATLM IFDSESL 
Sbjct: 61  LSCYVVFIIIKH-SILK-APRPVYLVDFSCLRPPSFCRIPFSFFLENATLMGIFDSESLI 120

Query: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 180
           FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINE HMVLFPVMDDLLTKTHLSP DID
Sbjct: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEVHMVLFPVMDDLLTKTHLSPPDID 180

Query: 181 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 240
           ILIVNCSGFCPSPSLSSI+IN+YSMRSDIKSFNLSGMGCSASAVAIHLAE+LLQVH+NSN
Sbjct: 181 ILIVNCSGFCPSPSLSSIIINRYSMRSDIKSFNLSGMGCSASAVAIHLAENLLQVHENSN 240

Query: 241 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 300
           AVVLSTEILSNGWYAGKE S+LILNC FRMG AAILLTNRKEAKD SKYKLFK++RT  +
Sbjct: 241 AVVLSTEILSNGWYAGKEPSRLILNCYFRMGGAAILLTNRKEAKDYSKYKLFKSLRTLTS 300

Query: 301 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 360
           FDDKSYLSA+REED DGKLGV++TRD LQV  ETLR NIT+LG S+LPLSEKL+H +SRL
Sbjct: 301 FDDKSYLSAMREEDKDGKLGVSVTRDTLQVFPETLRINITLLGSSILPLSEKLKHVISRL 360

Query: 361 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 420
            KRFLDKSQEIY+PNFK+VI H CLPVSGG VIR IGK+LKL DKD+EAALMTLHRFGNQ
Sbjct: 361 RKRFLDKSQEIYIPNFKTVINHICLPVSGGAVIRAIGKVLKLNDKDLEAALMTLHRFGNQ 420

Query: 421 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI- 480
           SSS+LWYELAY E +ER+ KGD+VWQ+GMGTGPKCVSLIWE IRPI GES  DPWA+VI 
Sbjct: 421 SSSALWYELAYFEAKERVKKGDKVWQIGMGTGPKCVSLIWECIRPISGESKKDPWAQVIH 480

Query: 481 QNPISGPPT 487
           Q PISGP T
Sbjct: 481 QYPISGPST 487

BLAST of MS000353 vs. ExPASy TrEMBL
Match: A0A6J1HWT8 (3-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111467383 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 9.4e-217
Identity = 389/489 (79.55%), Postives = 428/489 (87.53%), Query Frame = 0

Query: 1   MESAPKVIHGGTLLHSTQLLKPFRFTFFQLQILFV--LLTSIEAYLFLQGWNPIFHIFPL 60
           MESAPKV HGG  L S +L+     TFFQLQ  F+  L+ SIE+++FL+GWNP+FH+F L
Sbjct: 1   MESAPKVSHGGIALQSARLMTHLSLTFFQLQHFFILTLVISIESHIFLKGWNPMFHLFFL 60

Query: 61  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 120
            S  V  +I+     LN APRSVYLVDFSCLKPPSF RVPF+TFLENATLM IFDSESL 
Sbjct: 61  FSCFVIYMILRR-FFLN-APRSVYLVDFSCLKPPSFLRVPFATFLENATLMGIFDSESLN 120

Query: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 180
           FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINE HMVLFPVMDDLLTKTHLSP DID
Sbjct: 121 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEVHMVLFPVMDDLLTKTHLSPHDID 180

Query: 181 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 240
           ILIVNCSGFCPSPSLSSI+IN+YSMRSDIKSFNLSGMGCSASAVAIHLAE+LLQVH+NSN
Sbjct: 181 ILIVNCSGFCPSPSLSSIIINRYSMRSDIKSFNLSGMGCSASAVAIHLAENLLQVHENSN 240

Query: 241 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 300
           AVVLSTEILSNGWYAGKE S+LILNC FRMG AAILLTNRKEAKD SKYKLFK++RT  +
Sbjct: 241 AVVLSTEILSNGWYAGKEPSRLILNCYFRMGGAAILLTNRKEAKDYSKYKLFKSLRTLTS 300

Query: 301 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 360
           FDDKSYLSA+REED DGKLGV++TRD LQV  ETLR NIT+LG S+LPLSEKL+H +SRL
Sbjct: 301 FDDKSYLSAMREEDKDGKLGVSVTRDTLQVFPETLRINITLLGSSILPLSEKLKHVISRL 360

Query: 361 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 420
            KRFLDKSQEIY+PNFK+VI H CLPVSGG VIR IGK+LKL DKD+EAALMTLHRFGNQ
Sbjct: 361 RKRFLDKSQEIYIPNFKTVINHICLPVSGGAVIRAIGKVLKLNDKDLEAALMTLHRFGNQ 420

Query: 421 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQ 480
           SSS+LWYELAY E +ER+ KGD+VW +GMGTGPKCVSLIWE IRPI GES NDPWA+VI 
Sbjct: 421 SSSALWYELAYFEAKERVKKGDKVWHIGMGTGPKCVSLIWECIRPISGESKNDPWAQVIH 480

Query: 481 N-PISGPPT 487
           + PISGP T
Sbjct: 481 HYPISGPST 487

BLAST of MS000353 vs. ExPASy TrEMBL
Match: A0A5D3DEZ2 (3-ketoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold440G00030 PE=3 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 1.6e-216
Identity = 396/478 (82.85%), Postives = 424/478 (88.70%), Query Frame = 0

Query: 13  LLHSTQLL-KPFRFTFFQLQILFVLLTSIEAYL-FLQGWNPIFHIFPLSSLVVFIIIIHH 72
           L +STQLL  PF        IL  +LTSIEAYL F   W+P FHIFPLS LVV I     
Sbjct: 10  LRYSTQLLHTPF-------IILIPILTSIEAYLYFSHAWHPFFHIFPLSFLVVSI----- 69

Query: 73  PSILNIA-PRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFMAKTLKSSGQ 132
             I N+A P SVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSES+ FMA+TL SSGQ
Sbjct: 70  --IFNLASPNSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESISFMARTLMSSGQ 129

Query: 133 SEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDILIVNCSGFCP 192
           SE+TCLPPALHFIPPKTH QESINE H+VLFPVMDDLLTKTHLSP DIDILIVNCSGFCP
Sbjct: 130 SEQTCLPPALHFIPPKTHLQESINEVHIVLFPVMDDLLTKTHLSPSDIDILIVNCSGFCP 189

Query: 193 SPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAVVLSTEILSN 252
           SPSLSSIVINKYSMR+DIKSFNLSGMGCSASA+AIHLAE+LLQVHKNSNAVVLSTEILSN
Sbjct: 190 SPSLSSIVINKYSMRNDIKSFNLSGMGCSASAIAIHLAENLLQVHKNSNAVVLSTEILSN 249

Query: 253 GWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFDDKSYLSAIR 312
           GWYAGKERSKLILNCLFRMGSAAILL+N+ +AK+SSKYKL KT+RTQRAFDDKSYLS +R
Sbjct: 250 GWYAGKERSKLILNCLFRMGSAAILLSNKTQAKESSKYKLIKTLRTQRAFDDKSYLSGLR 309

Query: 313 EEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGKRFLDKSQEI 372
           EED DGKLGVTLTRDLL VAGETLRSNITILG S+LPLSEKLRHG SRL KRFLDKSQ+I
Sbjct: 310 EEDTDGKLGVTLTRDLLHVAGETLRSNITILGASILPLSEKLRHGFSRLRKRFLDKSQDI 369

Query: 373 YVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSSSSLWYELAY 432
           Y+PNFK+VIQHFCLPVSGG VIREIGK+LKL DKD+EAAL TLHRFGNQSSSSLWYELAY
Sbjct: 370 YIPNFKTVIQHFCLPVSGGAVIREIGKVLKLNDKDVEAALTTLHRFGNQSSSSLWYELAY 429

Query: 433 LEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVIQN-PISGPPT 487
           LE +ER+ KGDRVWQ+GMGTGPKCVSLIWE IRPI GESNN+PWA+VI   PI GPPT
Sbjct: 430 LEAKERVEKGDRVWQIGMGTGPKCVSLIWECIRPISGESNNEPWADVIDRYPILGPPT 473

BLAST of MS000353 vs. TAIR 10
Match: AT1G25450.1 (3-ketoacyl-CoA synthase 5 )

HSP 1 Score: 424.1 bits (1089), Expect = 1.5e-118
Identity = 211/427 (49.41%), Postives = 299/427 (70.02%), Query Frame = 0

Query: 52  IFHIFPLSSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDI 111
           + HI   S L++F+  ++  S     PR+VYLVD+SC KPP   RVPFS+F+E++ L+  
Sbjct: 59  LLHILCSSFLIIFVSTVYFMS----KPRTVYLVDYSCYKPPVTCRVPFSSFMEHSRLILK 118

Query: 112 FDSESLCFMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTH 171
            + +S+ F  + L+ SG  EETCLPPA+H+IPP    + + NEA MV+F  M+DL   T 
Sbjct: 119 DNPKSVEFQMRILERSGLGEETCLPPAIHYIPPTPTMESARNEAQMVIFTAMEDLFKNTG 178

Query: 172 LSPRDIDILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLL 231
           L P+DIDILIVNCS F P+PSLS+++INKY +RS+IKS+NLSGMGCSAS +++ +A  LL
Sbjct: 179 LKPKDIDILIVNCSLFSPTPSLSAMIINKYKLRSNIKSYNLSGMGCSASLISVDVARDLL 238

Query: 232 QVHKNSNAVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFK 291
           QVH NSNA+++STEI++  +Y G ER+ L+ NCLFRMG AAILL+NR+  +  +KYKL  
Sbjct: 239 QVHPNSNAIIISTEIITPNYYKGNERAMLLPNCLFRMGGAAILLSNRRSDRWRAKYKLCH 298

Query: 292 TVRTQRAFDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKL 351
            VRT R  DDKSY   + +ED +G +G+ L++DL+ +AGE L++NIT +G  VLP SE+L
Sbjct: 299 LVRTHRGADDKSYNCVMEQEDKNGNVGINLSKDLMTIAGEALKANITTIGPLVLPASEQL 358

Query: 352 RHGVSRLGKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMT 411
               S +G++  +   + Y+P+FK   +HFC+   G  VI E+ K L+L  + +EA+ MT
Sbjct: 359 LFLSSLIGRKIFNPKWKPYIPDFKQAFEHFCIHAGGRAVIDELQKNLQLSGEHVEASRMT 418

Query: 412 LHRFGNQSSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNND 471
           LHRFGN SSSSLWYEL+Y+E Q R+ + DRVWQ+  G+G KC S +W+  R I   ++  
Sbjct: 419 LHRFGNTSSSSLWYELSYIEAQGRMKRNDRVWQIAFGSGFKCNSAVWKCNRTIKTPTDG- 478

Query: 472 PWAEVIQ 479
            W++ I+
Sbjct: 479 AWSDCIE 480

BLAST of MS000353 vs. TAIR 10
Match: AT1G68530.1 (3-ketoacyl-CoA synthase 6 )

HSP 1 Score: 419.9 bits (1078), Expect = 2.9e-117
Identity = 212/436 (48.62%), Postives = 297/436 (68.12%), Query Frame = 0

Query: 46  LQGWN----PIFHIFPLSSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFST 105
           L  WN     +  +   S  V+FI  ++  S     PR++YLVD+SC KPP   RVPF+T
Sbjct: 54  LNVWNSLQFDLVQVLCSSFFVIFISTVYFMS----KPRTIYLVDYSCYKPPVTCRVPFAT 113

Query: 106 FLENATLMDIFDSESLCFMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFP 165
           F+E++ L+     +S+ F  + L+ SG  EETCLPPA+H+IPP      + +EA MV+F 
Sbjct: 114 FMEHSRLILKDKPKSVEFQMRILERSGLGEETCLPPAIHYIPPTPTMDAARSEAQMVIFE 173

Query: 166 VMDDLLTKTHLSPRDIDILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASA 225
            MDDL  KT L P+D+DILIVNCS F P+PSLS++VINKY +RS+IKSFNLSGMGCSA  
Sbjct: 174 AMDDLFKKTGLKPKDVDILIVNCSLFSPTPSLSAMVINKYKLRSNIKSFNLSGMGCSAGL 233

Query: 226 VAIHLAESLLQVHKNSNAVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEA 285
           +++ LA  LLQVH NSNA+++STEI++  +Y G ER+ L+ NCLFRMG+AAI ++NR+  
Sbjct: 234 ISVDLARDLLQVHPNSNAIIVSTEIITPNYYQGNERAMLLPNCLFRMGAAAIHMSNRRSD 293

Query: 286 KDSSKYKLFKTVRTQRAFDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILG 345
           +  +KYKL   VRT R  DDKS+     +ED +G +G+ L++DL+ +AGE L++NIT +G
Sbjct: 294 RWRAKYKLSHLVRTHRGADDKSFYCVYEQEDKEGHVGINLSKDLMAIAGEALKANITTIG 353

Query: 346 DSVLPLSEKLRHGVSRLGKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLK 405
             VLP SE+L    S +G++  +   + Y+P+FK   +HFC+   G  VI E+ K L+L 
Sbjct: 354 PLVLPASEQLLFLTSLIGRKIFNPKWKPYIPDFKLAFEHFCIHAGGRAVIDELQKNLQLS 413

Query: 406 DKDMEAALMTLHRFGNQSSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESI 465
            + +EA+ MTLHRFGN SSSSLWYEL+Y+E + R+ +GDRVWQ+  G+G KC S +W+  
Sbjct: 414 GEHVEASRMTLHRFGNTSSSSLWYELSYIESKGRMRRGDRVWQIAFGSGFKCNSAVWKCN 473

Query: 466 RPIVGESNNDPWAEVI 478
           R I     + PW++ I
Sbjct: 474 RTI-KTPKDGPWSDCI 484

BLAST of MS000353 vs. TAIR 10
Match: AT1G19440.1 (3-ketoacyl-CoA synthase 4 )

HSP 1 Score: 395.6 bits (1015), Expect = 5.8e-110
Identity = 192/419 (45.82%), Postives = 287/419 (68.50%), Query Frame = 0

Query: 59  SSLVVFIIIIHHPSILNIAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLC 118
           S+++VF + ++    +   PR VYLVDFSC  PP   + P++ F+E++ L   FD  +L 
Sbjct: 94  SAILVFGLTVY----VMTRPRPVYLVDFSCYLPPDHLKAPYARFMEHSRLTGDFDDSALE 153

Query: 119 FMAKTLKSSGQSEETCLPPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDID 178
           F  K L+ SG  E+T +P A+H++PP+     +  EA  V+F  +D+L   T++ P+DI 
Sbjct: 154 FQRKILERSGLGEDTYVPEAMHYVPPRISMAAAREEAEQVMFGALDNLFANTNVKPKDIG 213

Query: 179 ILIVNCSGFCPSPSLSSIVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSN 238
           IL+VNCS F P+PSLS++++NKY +R +I+S+NL GMGCSA  +A+ LA+ +L VH+N+ 
Sbjct: 214 ILVVNCSLFNPTPSLSAMIVNKYKLRGNIRSYNLGGMGCSAGVIAVDLAKDMLLVHRNTY 273

Query: 239 AVVLSTEILSNGWYAGKERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRA 298
           AVV+STE ++  WY G ++S LI NCLFR+G +A+LL+N+   K  SKY+L   VRT R 
Sbjct: 274 AVVVSTENITQNWYFGNKKSMLIPNCLFRVGGSAVLLSNKSRDKRRSKYRLVHVVRTHRG 333

Query: 299 FDDKSYLSAIREEDMDGKLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRL 358
            DDK++    +E+D  G+ GV+L++DL+ +AGETL++NIT LG  VLP+SE++   ++ +
Sbjct: 334 ADDKAFRCVYQEQDDTGRTGVSLSKDLMAIAGETLKTNITTLGPLVLPISEQILFFMTLV 393

Query: 359 GKRFLDKSQEIYVPNFKSVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQ 418
            K+  +   + Y+P+FK   +HFC+   G  VI E+ K L+L    +EA+ MTLHRFGN 
Sbjct: 394 VKKLFNGKVKPYIPDFKLAFEHFCIHAGGRAVIDELEKNLQLSPVHVEASRMTLHRFGNT 453

Query: 419 SSSSLWYELAYLEDQERIGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           SSSS+WYELAY+E + R+ +G+RVWQ+  G+G KC S IWE++R  V  SNN PW + I
Sbjct: 454 SSSSIWYELAYIEAKGRMRRGNRVWQIAFGSGFKCNSAIWEALRH-VKPSNNSPWEDCI 507

BLAST of MS000353 vs. TAIR 10
Match: AT2G16280.1 (3-ketoacyl-CoA synthase 9 )

HSP 1 Score: 381.3 bits (978), Expect = 1.1e-105
Identity = 203/462 (43.94%), Postives = 302/462 (65.37%), Query Frame = 0

Query: 25  FTFFQLQILFVLLTSI---------EAYLFLQGWNPIFHIFPLSSLVVFIIIIHHPSILN 84
           F    + ++ VL+T I         + +L LQ +N +  IF LS+L +F   ++  S   
Sbjct: 49  FKLCLVPLMAVLVTEISRLTTDDLYQIWLHLQ-YNLVAFIF-LSALAIFGSTVYIMS--- 108

Query: 85  IAPRSVYLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFMAKTLKSSGQSEETCL 144
             PRSVYLVD+SC  PP   +V +  F++++ L++ F+  SL F  K L+ SG  EET L
Sbjct: 109 -RPRSVYLVDYSCYLPPESLQVKYQKFMDHSKLIEDFNESSLEFQRKILERSGLGEETYL 168

Query: 145 PPALHFIPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDILIVNCSGFCPSPSLSS 204
           P ALH IPP+     +  E+  V+F  +D L   T ++PRDI +L+VNCS F P+PSLS+
Sbjct: 169 PEALHCIPPRPTMMAAREESEQVMFGALDKLFENTKINPRDIGVLVVNCSLFNPTPSLSA 228

Query: 205 IVINKYSMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAVVLSTEILSNGWYAGK 264
           +++NKY +R ++KSFNL GMGCSA  ++I LA+ +LQVH+N+ AVV+STE ++  WY G 
Sbjct: 229 MIVNKYKLRGNVKSFNLGGMGCSAGVISIDLAKDMLQVHRNTYAVVVSTENITQNWYFGN 288

Query: 265 ERSKLILNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFDDKSYLSAIREEDMDG 324
           +++ LI NCLFR+G +AILL+N+ + +  SKYKL  TVRT +   +K++    +E+D +G
Sbjct: 289 KKAMLIPNCLFRVGGSAILLSNKGKDRRRSKYKLVHTVRTHKGAVEKAFNCVYQEQDDNG 348

Query: 325 KLGVTLTRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGKRFLDKSQEIYVPNFK 384
           K GV+L++DL+ +AGE L++NIT LG  VLP+SE++   ++ + K+  +   + Y+P+FK
Sbjct: 349 KTGVSLSKDLMAIAGEALKANITTLGPLVLPISEQILFFMTLVTKKLFNSKLKPYIPDFK 408

Query: 385 SVIQHFCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSSSSLWYELAYLEDQER 444
               HFC+   G  VI E+ K L+L    +EA+ MTLHRFGN SSSS+WYELAY+E + R
Sbjct: 409 LAFDHFCIHAGGRAVIDELEKNLQLSQTHVEASRMTLHRFGNTSSSSIWYELAYIEAKGR 468

Query: 445 IGKGDRVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           + KG+RVWQ+  G+G KC S +W ++   V  S + PW   I
Sbjct: 469 MKKGNRVWQIAFGSGFKCNSAVWVALNN-VKPSVSSPWEHCI 503

BLAST of MS000353 vs. TAIR 10
Match: AT4G34510.1 (3-ketoacyl-CoA synthase 17 )

HSP 1 Score: 379.8 bits (974), Expect = 3.3e-105
Identity = 201/457 (43.98%), Postives = 295/457 (64.55%), Query Frame = 0

Query: 23  FRFTFFQLQILFVLLTSIEAYLFLQ-GWNPIFHIFPLSSLVVFIIIIHHPSILNIAPRSV 82
           F+  F  L  +  +  S+ +   LQ  +N    IF ++  +V  I+          PRS+
Sbjct: 28  FKLMFLPLMAVLFMNVSLLSLNHLQLYYNSTGFIFVITLAIVGSIV-----FFMSRPRSI 87

Query: 83  YLVDFSCLKPPSFFRVPFSTFLENATLMDIFDSESLCFMAKTLKSSGQSEETCLPPALHF 142
           YL+D+SC  PPS  +V +  F+ N++L+  F   SL F  K L  SG  EET LP ++H 
Sbjct: 88  YLLDYSCYLPPSSQKVSYQKFMNNSSLIQDFSETSLEFQRKILIRSGLGEETYLPDSIHS 147

Query: 143 IPPKTHQQESINEAHMVLFPVMDDLLTKTHLSPRDIDILIVNCSGFCPSPSLSSIVINKY 202
           IPP+     +  EA  V+F  +D+L   T ++PR+I +L+VNCS F P+PSLS++++NKY
Sbjct: 148 IPPRPTMAAAREEAEQVIFGALDNLFENTKINPREIGVLVVNCSLFNPTPSLSAMIVNKY 207

Query: 203 SMRSDIKSFNLSGMGCSASAVAIHLAESLLQVHKNSNAVVLSTEILSNGWYAGKERSKLI 262
            +R +IKSFNL GMGCSA  +A+ LA  +LQ+H+N+ A+V+STE ++  WY G +++ LI
Sbjct: 208 KLRGNIKSFNLGGMGCSAGVIAVDLASDMLQIHRNTFALVVSTENITQNWYFGNKKAMLI 267

Query: 263 LNCLFRMGSAAILLTNRKEAKDSSKYKLFKTVRTQRAFDDKSYLSAIREEDMDGKLGVTL 322
            NCLFR+G +A+LL+N+   +  SKYKL  TVRT +  D+ ++    +E+D   K GV+L
Sbjct: 268 PNCLFRVGGSAVLLSNKPLDRKRSKYKLVHTVRTHKGSDENAFNCVYQEQDECLKTGVSL 327

Query: 323 TRDLLQVAGETLRSNITILGDSVLPLSEKLRHGVSRLGKR-FLDKSQEIYVPNFKSVIQH 382
           ++DL+ +AGE L++NIT LG  VLP+SE++    + + KR F DK ++ Y+P+FK  + H
Sbjct: 328 SKDLMAIAGEALKTNITSLGPLVLPISEQILFFATFVAKRLFNDKKKKPYIPDFKLALDH 387

Query: 383 FCLPVSGGPVIREIGKMLKLKDKDMEAALMTLHRFGNQSSSSLWYELAYLEDQERIGKGD 442
           FC+   G  VI E+ K LKL  K +EA+ MTLHRFGN SSSS+WYELAY E + R+ KG+
Sbjct: 388 FCIHAGGRAVIDELEKSLKLSPKHVEASRMTLHRFGNTSSSSIWYELAYTEAKGRMRKGN 447

Query: 443 RVWQLGMGTGPKCVSLIWESIRPIVGESNNDPWAEVI 478
           RVWQ+  G+G KC S +W ++R  V  S N+PW   I
Sbjct: 448 RVWQIAFGSGFKCNSAVWVALRN-VEPSVNNPWEHCI 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140890.12.9e-27398.563-ketoacyl-CoA synthase 5-like [Momordica charantia][more]
XP_022140855.13.4e-22181.633-ketoacyl-CoA synthase 5-like [Momordica charantia][more]
XP_023542466.11.1e-21679.753-ketoacyl-CoA synthase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022968007.11.9e-21680.373-ketoacyl-CoA synthase 7-like [Cucurbita maxima][more]
XP_022968008.11.9e-21679.553-ketoacyl-CoA synthase 7-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9C6L52.1e-11749.413-ketoacyl-CoA synthase 5 OS=Arabidopsis thaliana OX=3702 GN=KCS5 PE=2 SV=1[more]
Q9XF434.1e-11648.623-ketoacyl-CoA synthase 6 OS=Arabidopsis thaliana OX=3702 GN=CUT1 PE=1 SV=1[more]
Q9LN498.2e-10945.823-ketoacyl-CoA synthase 4 OS=Arabidopsis thaliana OX=3702 GN=KCS4 PE=2 SV=1[more]
Q9SIX11.6e-10443.943-ketoacyl-CoA synthase 9 OS=Arabidopsis thaliana OX=3702 GN=KCS9 PE=2 SV=1[more]
O656774.6e-10443.983-ketoacyl-CoA synthase 17 OS=Arabidopsis thaliana OX=3702 GN=KCS17 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CGF61.4e-27398.563-ketoacyl-CoA synthase OS=Momordica charantia OX=3673 GN=LOC111011446 PE=3 SV=1[more]
A0A6J1CGC21.7e-22181.633-ketoacyl-CoA synthase OS=Momordica charantia OX=3673 GN=LOC111011424 PE=3 SV=1[more]
A0A6J1HSB59.4e-21780.373-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111467382 PE=3 SV=1[more]
A0A6J1HWT89.4e-21779.553-ketoacyl-CoA synthase OS=Cucurbita maxima OX=3661 GN=LOC111467383 PE=3 SV=1[more]
A0A5D3DEZ21.6e-21682.853-ketoacyl-CoA synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT1G25450.11.5e-11849.413-ketoacyl-CoA synthase 5 [more]
AT1G68530.12.9e-11748.623-ketoacyl-CoA synthase 6 [more]
AT1G19440.15.8e-11045.823-ketoacyl-CoA synthase 4 [more]
AT2G16280.11.1e-10543.943-ketoacyl-CoA synthase 9 [more]
AT4G34510.13.3e-10543.983-ketoacyl-CoA synthase 17 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePIRSFPIRSF036417Very_long_ch_3-ktacl-CoA_syncoord: 14..483
e-value: 4.7E-163
score: 541.1
IPR012392Very-long-chain 3-ketoacyl-CoA synthasePANTHERPTHR315613-KETOACYL-COA SYNTHASEcoord: 31..479
IPR013601FAE1/Type III polyketide synthase-like proteinPFAMPF08392FAE1_CUT1_RppAcoord: 77..359
e-value: 1.3E-109
score: 366.0
IPR0137473-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminalPFAMPF08541ACP_syn_III_Ccoord: 378..459
e-value: 1.6E-7
score: 31.3
IPR016039Thiolase-likeGENE3D3.40.47.10coord: 113..461
e-value: 1.5E-56
score: 194.0
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 266..461
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 73..280
NoneNo IPR availablePANTHERPTHR31561:SF273-KETOACYL-COA SYNTHASEcoord: 31..479
NoneNo IPR availableCDDcd00831CHS_likecoord: 80..458
e-value: 8.16634E-89
score: 274.102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000353.1MS000353.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
cellular_component GO:0016020 membrane
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups