MS000348 (gene) Bitter gourd (TR) v1

Overview
NameMS000348
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionembryo-specific protein ATS3B-like
Locationscaffold44: 1067348 .. 1068444 (+)
RNA-Seq ExpressionMS000348
SyntenyMS000348
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGGGCTCCGTCGCCTCTGCTGCTGCTGCTGCTGCTGCTATCTTCCCTCGCCGTCGTCCGCTCCGCCGCCGTTGTGTCGGCTCAGATCAAGACGCCTTCCGTCGCTCCCAAATCCCTTCCTATCAACTACATCCAGGTCTGTGTTTCAACTCTCTTTGTTCTCAACCGTCGATTTTGTAATTTTCTTTCGACTTTTTGTTATTTTACTTTTTTTTCTTCCCCTTTCGTGTCGCGAAGGAAGTCGGAAGCTGCGCCTACGAAGTTACCATAGCTACAAGCTGTTCTTCAGCTCCATTTACCACCGGCGAAATTGGTGTTCTGTTTGGAGATTCTCATGGAAATCAGGTTTTCCTTCTCTTCTGGCTCTTTCCTTTTTTCATATATGAAATTCCTGCTTGTTTACGGATCACTTTTAATCGAGACTGTGAAATGGTAGGGTTTTAAACTAGTAATCGTATGGTTTTTACTTTTTAACCTCTTTCTGAGAATAATTTTCCCCGAATCCAAACGAGACTGTAGGGTTTGTTTCTAATTTTATAAACGATGGTTCTCACCAGTTTTTGTTTTCCCAATCAATCTCTAAATAGAGATAAACTATTATTTTCCTTTGATGTTTGTTTTGTGCTATTTTGATCTGATGAAATGATTTCACATCGGAAATAGATCATCGAGAAAAAGTTGAATAACGGAGACAAGGTGTTTGATAGTTGTAGCACAGATAGCTTTGTTCTAAAGGATCGACCCTGCAGTGTTGGAATCAGTTATATGTATATCTACAAAGATGGGGCTGAAAATTGGGTTCCAAAATCTGTTGAAATCTCTGGCTCTGAAACCGAGCCCCTTCTGTTCAACTTCGATTCCTCCATCCCAAACAACACTTGGTTTGGCTTTGATGTGCCTCCATATCCTTTCCCGCCGCTGTCCGACGACTCGCCCCCACCTTTTCCCTGGTTGTCTCCTCCGCCACCGCCGCCTGAGCCACCAGTGGAGCCTGTGTTTCCCCCACCGCTTCCTCCATCGTCCTCCAGCAAACTATCAGGTCAGAAATGGGGCATCATAGCCGGGATTCTGGGATTGCTGATTGTTGCTCTT

mRNA sequence

ATGGAGAGGGCTCCGTCGCCTCTGCTGCTGCTGCTGCTGCTGCTATCTTCCCTCGCCGTCGTCCGCTCCGCCGCCGTTGTGTCGGCTCAGATCAAGACGCCTTCCGTCGCTCCCAAATCCCTTCCTATCAACTACATCCAGGAAGTCGGAAGCTGCGCCTACGAAGTTACCATAGCTACAAGCTGTTCTTCAGCTCCATTTACCACCGGCGAAATTGGTGTTCTGTTTGGAGATTCTCATGGAAATCAGATCATCGAGAAAAAGTTGAATAACGGAGACAAGGTGTTTGATAGTTGTAGCACAGATAGCTTTGTTCTAAAGGATCGACCCTGCAGTGTTGGAATCAGTTATATGTATATCTACAAAGATGGGGCTGAAAATTGGGTTCCAAAATCTGTTGAAATCTCTGGCTCTGAAACCGAGCCCCTTCTGTTCAACTTCGATTCCTCCATCCCAAACAACACTTGGTTTGGCTTTGATGTGCCTCCATATCCTTTCCCGCCGCTGTCCGACGACTCGCCCCCACCTTTTCCCTGGTTGTCTCCTCCGCCACCGCCGCCTGAGCCACCAGTGGAGCCTGTGTTTCCCCCACCGCTTCCTCCATCGTCCTCCAGCAAACTATCAGGTCAGAAATGGGGCATCATAGCCGGGATTCTGGGATTGCTGATTGTTGCTCTT

Coding sequence (CDS)

ATGGAGAGGGCTCCGTCGCCTCTGCTGCTGCTGCTGCTGCTGCTATCTTCCCTCGCCGTCGTCCGCTCCGCCGCCGTTGTGTCGGCTCAGATCAAGACGCCTTCCGTCGCTCCCAAATCCCTTCCTATCAACTACATCCAGGAAGTCGGAAGCTGCGCCTACGAAGTTACCATAGCTACAAGCTGTTCTTCAGCTCCATTTACCACCGGCGAAATTGGTGTTCTGTTTGGAGATTCTCATGGAAATCAGATCATCGAGAAAAAGTTGAATAACGGAGACAAGGTGTTTGATAGTTGTAGCACAGATAGCTTTGTTCTAAAGGATCGACCCTGCAGTGTTGGAATCAGTTATATGTATATCTACAAAGATGGGGCTGAAAATTGGGTTCCAAAATCTGTTGAAATCTCTGGCTCTGAAACCGAGCCCCTTCTGTTCAACTTCGATTCCTCCATCCCAAACAACACTTGGTTTGGCTTTGATGTGCCTCCATATCCTTTCCCGCCGCTGTCCGACGACTCGCCCCCACCTTTTCCCTGGTTGTCTCCTCCGCCACCGCCGCCTGAGCCACCAGTGGAGCCTGTGTTTCCCCCACCGCTTCCTCCATCGTCCTCCAGCAAACTATCAGGTCAGAAATGGGGCATCATAGCCGGGATTCTGGGATTGCTGATTGTTGCTCTT

Protein sequence

MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIATSCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYIYKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPFPPLSDDSPPPFPWLSPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVAL
Homology
BLAST of MS000348 vs. NCBI nr
Match: XP_022140834.1 (embryo-specific protein ATS3B-like [Momordica charantia])

HSP 1 Score: 426.8 bits (1096), Expect = 1.2e-115
Identity = 222/226 (98.23%), Postives = 223/226 (98.67%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MERAPSPLLLLLLLLSSLAVVRSAAVVSA IKTPSVAPKSLPINYIQEVGSCAYEVTIAT
Sbjct: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAPIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI
Sbjct: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPFPPLSDDSPPPFPWL 180
           YKDGAENWVPKSVEISGSETEPLLFNF+SSIPNNTWFGFDVPPY FPP SDDSPPPFPWL
Sbjct: 121 YKDGAENWVPKSVEISGSETEPLLFNFNSSIPNNTWFGFDVPPYTFPPPSDDSPPPFPWL 180

Query: 181 SPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVAL 227
           SPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVAL
Sbjct: 181 SPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVAL 226

BLAST of MS000348 vs. NCBI nr
Match: XP_038877437.1 (embryo-specific protein ATS3B-like [Benincasa hispida])

HSP 1 Score: 277.3 bits (708), Expect = 1.2e-70
Identity = 156/241 (64.73%), Postives = 181/241 (75.10%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           ME+APSP LLLLLLLS LAV+ SA   +     P VAP S+PINYIQ+VGSC+Y+VT+ T
Sbjct: 1   MEKAPSP-LLLLLLLSFLAVICSAVEPAPITVKPDVAPVSIPINYIQKVGSCSYDVTVVT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SC+S+   TGEIG+LFGDSHGNQIIEKKL+N DKVFDSC+TDSFVLKDRPCSV +SYMYI
Sbjct: 61  SCASSSSITGEIGILFGDSHGNQIIEKKLSNEDKVFDSCNTDSFVLKDRPCSVQMSYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPFP-PLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +PLLF F SSIP NTWFGFD+  +PFP P S D  PP PW
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPLLFIFKSSIPRNTWFGFDLRQFPFPSPPSYDPAPPLPW 180

Query: 181 LSPPPPPPE-----PPVEPVFPPP-------------LPPSSSSKLSGQKWGIIAGILGL 223
           L PPP PP      PP EP+ PPP              PPSSSSK+SG  WG+++ ILGL
Sbjct: 181 LFPPPSPPPSFPLLPPPEPILPPPPPPEPVLPPVPPSTPPSSSSKISGHAWGMVSVILGL 240

BLAST of MS000348 vs. NCBI nr
Match: KAG6576773.1 (Embryo-specific protein ATS3B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 275.4 bits (703), Expect = 4.4e-70
Identity = 161/246 (65.45%), Postives = 181/246 (73.58%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MER+ SP L LLLLLS+LAVV SA         P VAP+SLPINYIQEVGSC+YEVT+AT
Sbjct: 1   MERSSSPPLSLLLLLSALAVVCSAVEPPQITVKPDVAPESLPINYIQEVGSCSYEVTVAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSS  F T EIGVLFGDSHGNQI+EKKL+NGDKVFDSC+TD FVL DRPCSV ISYMYI
Sbjct: 61  SCSSPSFITDEIGVLFGDSHGNQIVEKKLSNGDKVFDSCNTDRFVLNDRPCSVQISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPF-PPLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +PLLF F SSIP+NTWFGFD+  YPF  P S +  PPFP 
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPLLFIFKSSIPSNTWFGFDLRQYPFLSPPSYNPYPPFPS 180

Query: 181 LSPPPPPP-----EPPVEPVF--------------PPPLPPSSSSKLSGQKWGIIAGILG 227
           LSPPPPPP      PP EP+F              PPP PPSSSSK+SG K   ++ +LG
Sbjct: 181 LSPPPPPPPPHPLPPPPEPIFSPPPPPKPVLPPSPPPPPPPSSSSKISGLKLAKVSVVLG 240

BLAST of MS000348 vs. NCBI nr
Match: XP_022984443.1 (embryo-specific protein ATS3B-like [Cucurbita maxima])

HSP 1 Score: 275.0 bits (702), Expect = 5.7e-70
Identity = 160/247 (64.78%), Postives = 179/247 (72.47%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MER+ SP LLLLLLLSSLAVV SA         P +AP+SLPINYIQEVGSC+YEVT+AT
Sbjct: 1   MERSSSPPLLLLLLLSSLAVVCSAVEPPQITVKPDIAPQSLPINYIQEVGSCSYEVTVAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSS  F T EIGVLFGDSHGNQI+EKKL+NGDKVFDSC+TD FVL DRPCSV ISYMYI
Sbjct: 61  SCSSPSFITDEIGVLFGDSHGNQIVEKKLSNGDKVFDSCNTDRFVLNDRPCSVQISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPFP-PLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +PLLF   SSIP N WFGFD+  YPFP P S +  PPFP 
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPLLFILKSSIPRNNWFGFDLRQYPFPSPPSYNPSPPFPS 180

Query: 181 LSPPPPPP---------EPPVEPVF-----------PPPLPPSSSSKLSGQKWGIIAGIL 227
           LSPPPPPP          PP EP+            PPP PPSSSSK+SG K   ++ +L
Sbjct: 181 LSPPPPPPPPPPPHHPLPPPPEPILPPPPPPKPVLPPPPPPPSSSSKISGLKLAKVSVVL 240

BLAST of MS000348 vs. NCBI nr
Match: XP_022923122.1 (embryo-specific protein ATS3B-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 273.1 bits (697), Expect = 2.2e-69
Identity = 160/247 (64.78%), Postives = 181/247 (73.28%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MER+ SP LLLLLLLS+LAVV SA         P VAP+SL INYIQEVGSC+YEVT+AT
Sbjct: 1   MERSSSPPLLLLLLLSALAVVCSAVEPPQITVKPDVAPESLQINYIQEVGSCSYEVTVAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSS  F T EIGVLFGDSHGNQI+EKKL+NGDKVFDSC+TD FVL DRPCSV ISYMYI
Sbjct: 61  SCSSPSFITDEIGVLFGDSHGNQIVEKKLSNGDKVFDSCNTDRFVLNDRPCSVQISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPF-PPLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +P+LF F SSIP+NTWFGFD+  YPF  P S +  PPFP 
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPVLFIFKSSIPSNTWFGFDLRQYPFLSPPSYNPYPPFPS 180

Query: 181 LSPPPPPP---------EPPVEPVF-----------PPPLPPSSSSKLSGQKWGIIAGIL 227
           LSPPPPPP          PP EP+F           PPP PPSSSSK+SG K   ++ +L
Sbjct: 181 LSPPPPPPPPPPPPHPLPPPPEPIFSPPPPPKPVLPPPPPPPSSSSKISGLKLAKVSVVL 240

BLAST of MS000348 vs. ExPASy Swiss-Prot
Match: Q6NPM5 (Embryo-specific protein ATS3B OS=Arabidopsis thaliana OX=3702 GN=ATS3B PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 7.1e-23
Identity = 53/152 (34.87%), Postives = 94/152 (61.84%), Query Frame = 0

Query: 11  LLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVG-SCAYEVTIATSCSSAPFTT 70
           L   L S   + S +V  +++  P    +S  ++ IQ++G +CAY V I+TSCSS  +T 
Sbjct: 6   LFFTLISFVFIISTSVYESKVLDPPHVAESFNVSLIQKLGNTCAYTVIISTSCSSTRYTR 65

Query: 71  GEIGVLFGDSHGNQIIEKKLNN-GDKVFDSCSTDSFVLKDRPCSVGISYMYIYKDGAENW 130
            +I V FGD +GNQI   +L++   K F+ CS+D+F + + PC+  I Y+Y+Y+ G + W
Sbjct: 66  DQISVAFGDGYGNQIYAPRLDDPSTKTFEQCSSDTFQI-NGPCTYQICYVYLYRSGPDGW 125

Query: 131 VPKSVEISGSETEPLLFNFDSSIPNNTWFGFD 161
           +P +V+I    ++ + F +++ +P + W+GF+
Sbjct: 126 IPNTVKIYSHGSKAVTFPYNTYVPESVWYGFN 156

BLAST of MS000348 vs. ExPASy Swiss-Prot
Match: Q681K2 (Embryo-specific protein ATS3A OS=Arabidopsis thaliana OX=3702 GN=ATS3A PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.8e-19
Identity = 50/162 (30.86%), Postives = 90/162 (55.56%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           M R   PL L  L   +L     + + +  +   S  PK      ++   +C+Y V I T
Sbjct: 1   MLRLAIPLFLFALCSFTLFSSARSFITTKPLPIDSFIPKP----KLENAAACSYTVIIKT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNN-GDKVFDSCSTDSFVLKDRPCSVGISYMY 120
           SCSS  +T  +I + FGD +GN++  K+L++   + F+ CS+D++ +   PC   + Y+Y
Sbjct: 61  SCSSVSYTRDKISISFGDVYGNEVYVKRLDDPSSRTFEKCSSDTYKISG-PCMRDVCYLY 120

Query: 121 IYKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDV 162
           + + G++ W P++V+I GS    + F ++  +PN+ W+GF+V
Sbjct: 121 LLRQGSDGWKPENVKIYGSSIRSVTFYYNLFLPNSVWYGFNV 157

BLAST of MS000348 vs. ExPASy Swiss-Prot
Match: Q9LYP6 (Embryo-specific protein ATS3 OS=Arabidopsis thaliana OX=3702 GN=ATS3 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.7e-16
Identity = 63/181 (34.81%), Postives = 97/181 (53.59%), Query Frame = 0

Query: 50  GSCAYEVTIATSCSSAPFTTGEIGVLFGDSHGNQIIEKKLN---NGDKVFDSCSTDSFVL 109
           G+C Y V + TSC S   T  +I ++FGD+ GN++   KL     G      CST++F +
Sbjct: 32  GTCPYTVVVMTSCLSPESTRDQISIVFGDADGNKVYAPKLGGLVRGPGGLGKCSTNTFQV 91

Query: 110 KDRPCSVGISYMYIYKDGAENWVPKSVEISGSETEPLLFNFDSSIPN-NTWFGFDVPPYP 169
           + +  +  I  +YI ++G + WVP+S+EI    ++ + F+F  S+P  NTW+G +     
Sbjct: 92  RGQCLNDPICSLYINRNGPDGWVPESIEIYSEGSKSVKFDFSKSVPQLNTWYGHNNCNTT 151

Query: 170 FPPLSDDSPPPFPWLSPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVA 227
             P S D PPP     PP  PPE P  P  PPP  PS++S+L   +   +A  +   I A
Sbjct: 152 GRPSSPDLPPPH---FPPEFPPETPTTPP-PPPPRPSAASRLGNGESVFLAFAIATAIAA 208

BLAST of MS000348 vs. ExPASy TrEMBL
Match: A0A6J1CGW3 (embryo-specific protein ATS3B-like OS=Momordica charantia OX=3673 GN=LOC111011405 PE=4 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 5.7e-116
Identity = 222/226 (98.23%), Postives = 223/226 (98.67%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MERAPSPLLLLLLLLSSLAVVRSAAVVSA IKTPSVAPKSLPINYIQEVGSCAYEVTIAT
Sbjct: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAPIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI
Sbjct: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPFPPLSDDSPPPFPWL 180
           YKDGAENWVPKSVEISGSETEPLLFNF+SSIPNNTWFGFDVPPY FPP SDDSPPPFPWL
Sbjct: 121 YKDGAENWVPKSVEISGSETEPLLFNFNSSIPNNTWFGFDVPPYTFPPPSDDSPPPFPWL 180

Query: 181 SPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVAL 227
           SPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVAL
Sbjct: 181 SPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVAL 226

BLAST of MS000348 vs. ExPASy TrEMBL
Match: A0A6J1JAH7 (embryo-specific protein ATS3B-like OS=Cucurbita maxima OX=3661 GN=LOC111482743 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.8e-70
Identity = 160/247 (64.78%), Postives = 179/247 (72.47%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MER+ SP LLLLLLLSSLAVV SA         P +AP+SLPINYIQEVGSC+YEVT+AT
Sbjct: 1   MERSSSPPLLLLLLLSSLAVVCSAVEPPQITVKPDIAPQSLPINYIQEVGSCSYEVTVAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSS  F T EIGVLFGDSHGNQI+EKKL+NGDKVFDSC+TD FVL DRPCSV ISYMYI
Sbjct: 61  SCSSPSFITDEIGVLFGDSHGNQIVEKKLSNGDKVFDSCNTDRFVLNDRPCSVQISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPFP-PLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +PLLF   SSIP N WFGFD+  YPFP P S +  PPFP 
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPLLFILKSSIPRNNWFGFDLRQYPFPSPPSYNPSPPFPS 180

Query: 181 LSPPPPPP---------EPPVEPVF-----------PPPLPPSSSSKLSGQKWGIIAGIL 227
           LSPPPPPP          PP EP+            PPP PPSSSSK+SG K   ++ +L
Sbjct: 181 LSPPPPPPPPPPPHHPLPPPPEPILPPPPPPKPVLPPPPPPPSSSSKISGLKLAKVSVVL 240

BLAST of MS000348 vs. ExPASy TrEMBL
Match: A0A6J1E5A6 (embryo-specific protein ATS3B-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111430882 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 1.1e-69
Identity = 160/247 (64.78%), Postives = 181/247 (73.28%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MER+ SP LLLLLLLS+LAVV SA         P VAP+SL INYIQEVGSC+YEVT+AT
Sbjct: 1   MERSSSPPLLLLLLLSALAVVCSAVEPPQITVKPDVAPESLQINYIQEVGSCSYEVTVAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSS  F T EIGVLFGDSHGNQI+EKKL+NGDKVFDSC+TD FVL DRPCSV ISYMYI
Sbjct: 61  SCSSPSFITDEIGVLFGDSHGNQIVEKKLSNGDKVFDSCNTDRFVLNDRPCSVQISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPF-PPLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +P+LF F SSIP+NTWFGFD+  YPF  P S +  PPFP 
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPVLFIFKSSIPSNTWFGFDLRQYPFLSPPSYNPYPPFPS 180

Query: 181 LSPPPPPP---------EPPVEPVF-----------PPPLPPSSSSKLSGQKWGIIAGIL 227
           LSPPPPPP          PP EP+F           PPP PPSSSSK+SG K   ++ +L
Sbjct: 181 LSPPPPPPPPPPPPHPLPPPPEPIFSPPPPPKPVLPPPPPPPSSSSKISGLKLAKVSVVL 240

BLAST of MS000348 vs. ExPASy TrEMBL
Match: A0A6J1E5Y4 (embryo-specific protein ATS3B-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430882 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 9.8e-68
Identity = 160/264 (60.61%), Postives = 181/264 (68.56%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MER+ SP LLLLLLLS+LAVV SA         P VAP+SL INYIQEVGSC+YEVT+AT
Sbjct: 1   MERSSSPPLLLLLLLSALAVVCSAVEPPQITVKPDVAPESLQINYIQEVGSCSYEVTVAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSS  F T EIGVLFGDSHGNQI+EKKL+NGDKVFDSC+TD FVL DRPCSV ISYMYI
Sbjct: 61  SCSSPSFITDEIGVLFGDSHGNQIVEKKLSNGDKVFDSCNTDRFVLNDRPCSVQISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPF-PPLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +P+LF F SSIP+NTWFGFD+  YPF  P S +  PPFP 
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPVLFIFKSSIPSNTWFGFDLRQYPFLSPPSYNPYPPFPS 180

Query: 181 LSPPPPPP---------EPPVEPVF----------------------------PPPLPPS 227
           LSPPPPPP          PP EP+F                            PPP PPS
Sbjct: 181 LSPPPPPPPPPPPPHPLPPPPEPIFSPPPPPKPVLPPPPPPPPPKPVLPPPPPPPPPPPS 240

BLAST of MS000348 vs. ExPASy TrEMBL
Match: A0A6J1EAT0 (embryo-specific protein ATS3B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430882 PE=4 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.4e-66
Identity = 160/274 (58.39%), Postives = 181/274 (66.06%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           MER+ SP LLLLLLLS+LAVV SA         P VAP+SL INYIQEVGSC+YEVT+AT
Sbjct: 1   MERSSSPPLLLLLLLSALAVVCSAVEPPQITVKPDVAPESLQINYIQEVGSCSYEVTVAT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNNGDKVFDSCSTDSFVLKDRPCSVGISYMYI 120
           SCSS  F T EIGVLFGDSHGNQI+EKKL+NGDKVFDSC+TD FVL DRPCSV ISYMYI
Sbjct: 61  SCSSPSFITDEIGVLFGDSHGNQIVEKKLSNGDKVFDSCNTDRFVLNDRPCSVQISYMYI 120

Query: 121 YKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDVPPYPF-PPLSDDSPPPFPW 180
           YKDGA++W+P SVEISGS  +P+LF F SSIP+NTWFGFD+  YPF  P S +  PPFP 
Sbjct: 121 YKDGADDWLPNSVEISGSGIKPVLFIFKSSIPSNTWFGFDLRQYPFLSPPSYNPYPPFPS 180

Query: 181 LSPPPPPP------------------------------------EPPVEPVF-------- 227
           LSPPPPPP                                     PP EP+F        
Sbjct: 181 LSPPPPPPPPHPLPPPPEPILSPPPPPKPVLPPPSPPPPPPHPLPPPPEPIFSPPPPPKP 240

BLAST of MS000348 vs. TAIR 10
Match: AT5G62200.1 (Embryo-specific protein 3, (ATS3) )

HSP 1 Score: 109.0 bits (271), Expect = 5.1e-24
Identity = 53/152 (34.87%), Postives = 94/152 (61.84%), Query Frame = 0

Query: 11  LLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVG-SCAYEVTIATSCSSAPFTT 70
           L   L S   + S +V  +++  P    +S  ++ IQ++G +CAY V I+TSCSS  +T 
Sbjct: 6   LFFTLISFVFIISTSVYESKVLDPPHVAESFNVSLIQKLGNTCAYTVIISTSCSSTRYTR 65

Query: 71  GEIGVLFGDSHGNQIIEKKLNN-GDKVFDSCSTDSFVLKDRPCSVGISYMYIYKDGAENW 130
            +I V FGD +GNQI   +L++   K F+ CS+D+F + + PC+  I Y+Y+Y+ G + W
Sbjct: 66  DQISVAFGDGYGNQIYAPRLDDPSTKTFEQCSSDTFQI-NGPCTYQICYVYLYRSGPDGW 125

Query: 131 VPKSVEISGSETEPLLFNFDSSIPNNTWFGFD 161
           +P +V+I    ++ + F +++ +P + W+GF+
Sbjct: 126 IPNTVKIYSHGSKAVTFPYNTYVPESVWYGFN 156

BLAST of MS000348 vs. TAIR 10
Match: AT5G62210.1 (Embryo-specific protein 3, (ATS3) )

HSP 1 Score: 102.8 bits (255), Expect = 3.6e-22
Identity = 67/177 (37.85%), Postives = 103/177 (58.19%), Query Frame = 0

Query: 46  IQEVGSCAYEVTIATSCSSAPFTTGEIGVLFGDSHGNQIIEKKLN---NGDKVFDSCSTD 105
           + E  SC Y V + TSC S  ++  ++ +  GD+  NQ++  +L+   +G   F+ CS+D
Sbjct: 34  LHEEESCPYTVIVTTSCFSPDWSRDQVTIALGDADDNQVVAPRLDKPLSGGGGFEKCSSD 93

Query: 106 SFVLKDRPCSVGISYMYIYKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDV- 165
           +F +K + C   I  +YIY+ G + W+P++VEI    ++ + F+F+ ++P N W+G +  
Sbjct: 94  TFQVKGK-CLNTICSVYIYRSGTDGWIPETVEIYKEGSKSVKFDFNKNVPENIWYGNNYC 153

Query: 166 ------PPYP-FPPLSDDSPPPFPWLSPPPPPPEPPVEPVFPPPLPPSSSS-KLSGQ 211
                 PP P FPP S   PPP    SPP  PPEPP  P  PPP PPS++S + SGQ
Sbjct: 154 NNTDLPPPSPDFPPFSPSIPPP----SPPYFPPEPPSIPPPPPPSPPSAASGRGSGQ 205

BLAST of MS000348 vs. TAIR 10
Match: AT2G41475.1 (Embryo-specific protein 3, (ATS3) )

HSP 1 Score: 97.1 bits (240), Expect = 2.0e-20
Identity = 50/162 (30.86%), Postives = 90/162 (55.56%), Query Frame = 0

Query: 1   MERAPSPLLLLLLLLSSLAVVRSAAVVSAQIKTPSVAPKSLPINYIQEVGSCAYEVTIAT 60
           M R   PL L  L   +L     + + +  +   S  PK      ++   +C+Y V I T
Sbjct: 1   MLRLAIPLFLFALCSFTLFSSARSFITTKPLPIDSFIPKP----KLENAAACSYTVIIKT 60

Query: 61  SCSSAPFTTGEIGVLFGDSHGNQIIEKKLNN-GDKVFDSCSTDSFVLKDRPCSVGISYMY 120
           SCSS  +T  +I + FGD +GN++  K+L++   + F+ CS+D++ +   PC   + Y+Y
Sbjct: 61  SCSSVSYTRDKISISFGDVYGNEVYVKRLDDPSSRTFEKCSSDTYKISG-PCMRDVCYLY 120

Query: 121 IYKDGAENWVPKSVEISGSETEPLLFNFDSSIPNNTWFGFDV 162
           + + G++ W P++V+I GS    + F ++  +PN+ W+GF+V
Sbjct: 121 LLRQGSDGWKPENVKIYGSSIRSVTFYYNLFLPNSVWYGFNV 157

BLAST of MS000348 vs. TAIR 10
Match: AT5G07190.1 (seed gene 3 )

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-17
Identity = 63/181 (34.81%), Postives = 97/181 (53.59%), Query Frame = 0

Query: 50  GSCAYEVTIATSCSSAPFTTGEIGVLFGDSHGNQIIEKKLN---NGDKVFDSCSTDSFVL 109
           G+C Y V + TSC S   T  +I ++FGD+ GN++   KL     G      CST++F +
Sbjct: 32  GTCPYTVVVMTSCLSPESTRDQISIVFGDADGNKVYAPKLGGLVRGPGGLGKCSTNTFQV 91

Query: 110 KDRPCSVGISYMYIYKDGAENWVPKSVEISGSETEPLLFNFDSSIPN-NTWFGFDVPPYP 169
           + +  +  I  +YI ++G + WVP+S+EI    ++ + F+F  S+P  NTW+G +     
Sbjct: 92  RGQCLNDPICSLYINRNGPDGWVPESIEIYSEGSKSVKFDFSKSVPQLNTWYGHNNCNTT 151

Query: 170 FPPLSDDSPPPFPWLSPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVA 227
             P S D PPP     PP  PPE P  P  PPP  PS++S+L   +   +A  +   I A
Sbjct: 152 GRPSSPDLPPPH---FPPEFPPETPTTPP-PPPPRPSAASRLGNGESVFLAFAIATAIAA 208

BLAST of MS000348 vs. TAIR 10
Match: AT5G07190.2 (seed gene 3 )

HSP 1 Score: 87.8 bits (216), Expect = 1.2e-17
Identity = 63/181 (34.81%), Postives = 97/181 (53.59%), Query Frame = 0

Query: 50  GSCAYEVTIATSCSSAPFTTGEIGVLFGDSHGNQIIEKKLN---NGDKVFDSCSTDSFVL 109
           G+C Y V + TSC S   T  +I ++FGD+ GN++   KL     G      CST++F +
Sbjct: 4   GTCPYTVVVMTSCLSPESTRDQISIVFGDADGNKVYAPKLGGLVRGPGGLGKCSTNTFQV 63

Query: 110 KDRPCSVGISYMYIYKDGAENWVPKSVEISGSETEPLLFNFDSSIPN-NTWFGFDVPPYP 169
           + +  +  I  +YI ++G + WVP+S+EI    ++ + F+F  S+P  NTW+G +     
Sbjct: 64  RGQCLNDPICSLYINRNGPDGWVPESIEIYSEGSKSVKFDFSKSVPQLNTWYGHNNCNTT 123

Query: 170 FPPLSDDSPPPFPWLSPPPPPPEPPVEPVFPPPLPPSSSSKLSGQKWGIIAGILGLLIVA 227
             P S D PPP     PP  PPE P  P  PPP  PS++S+L   +   +A  +   I A
Sbjct: 124 GRPSSPDLPPPH---FPPEFPPETPTTPP-PPPPRPSAASRLGNGESVFLAFAIATAIAA 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140834.11.2e-11598.23embryo-specific protein ATS3B-like [Momordica charantia][more]
XP_038877437.11.2e-7064.73embryo-specific protein ATS3B-like [Benincasa hispida][more]
KAG6576773.14.4e-7065.45Embryo-specific protein ATS3B, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022984443.15.7e-7064.78embryo-specific protein ATS3B-like [Cucurbita maxima][more]
XP_022923122.12.2e-6964.78embryo-specific protein ATS3B-like isoform X4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q6NPM57.1e-2334.87Embryo-specific protein ATS3B OS=Arabidopsis thaliana OX=3702 GN=ATS3B PE=1 SV=1[more]
Q681K22.8e-1930.86Embryo-specific protein ATS3A OS=Arabidopsis thaliana OX=3702 GN=ATS3A PE=1 SV=1[more]
Q9LYP61.7e-1634.81Embryo-specific protein ATS3 OS=Arabidopsis thaliana OX=3702 GN=ATS3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CGW35.7e-11698.23embryo-specific protein ATS3B-like OS=Momordica charantia OX=3673 GN=LOC11101140... [more]
A0A6J1JAH72.8e-7064.78embryo-specific protein ATS3B-like OS=Cucurbita maxima OX=3661 GN=LOC111482743 P... [more]
A0A6J1E5A61.1e-6964.78embryo-specific protein ATS3B-like isoform X4 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1E5Y49.8e-6860.61embryo-specific protein ATS3B-like isoform X3 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1EAT01.4e-6658.39embryo-specific protein ATS3B-like isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G62200.15.1e-2434.87Embryo-specific protein 3, (ATS3) [more]
AT5G62210.13.6e-2237.85Embryo-specific protein 3, (ATS3) [more]
AT2G41475.12.0e-2030.86Embryo-specific protein 3, (ATS3) [more]
AT5G07190.11.2e-1734.81seed gene 3 [more]
AT5G07190.21.2e-1734.81seed gene 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010417Embryo-specific ATS3PFAMPF06232ATS3coord: 48..161
e-value: 9.2E-30
score: 103.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..203
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 175..208
NoneNo IPR availablePANTHERPTHR31718FAMILY NOT NAMEDcoord: 9..223
NoneNo IPR availablePANTHERPTHR31718:SF32EMBRYO-SPECIFIC PROTEIN ATS3Bcoord: 9..223
IPR036392PLAT/LH2 domain superfamilySUPERFAMILY49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 49..158

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000348.1MS000348.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding