MS000318 (gene) Bitter gourd (TR) v1

Overview
NameMS000318
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionmonocopper oxidase-like protein SKS1
Locationscaffold44: 877744 .. 882116 (+)
RNA-Seq ExpressionMS000318
SyntenyMS000318
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCACTGTTTCATCTCTTTCCACTGCTGCTACTGCTTATGCTTCCGTCGCTCTGTTCCGCCGCTGATCCCTACATCTTTTACGAATTTCGCGTCTCTTACATTACTGCCTCTCCCTTGGGCGTTCCTCAACAGGTTCTCTATCTCGCTCTTCTTATGCAACTTTCCGGCTTCCTCTGTTGTGTTCTGTTTCGCTTTTTGCATATTTTCAGATTGAACTGTGCCTTTCTGAATGGTTTCCTCTAGTGTTTGGCTCAAGAGTTTATGTTGTTTGAGGAGTTTTTCTTTTGGAAGTAAGGGTTTAAGGTCTAACTTCCTATTTAGTGATTTTGATTTTTTTCTGCGTGACTCTCAATGAGCGTGAGGAGTTGTTCTGATAATTTTTTGGAGTCTGATTCTGGGAAGTAAAATGGTGAACTTGATTATTATTGATTTTCTCTTGGCTTTAACGCTTTCTGCCTTAAAATTTTGTTTTTGAATCTAGTTATTGGTGTTCTATTGTTTTGCTCTGTAAATCACATAAACAGGAAATGGATGCTGGTGAGAAAAACTGTTTATTTTATTTTGTAGCAATATAGTTTTGTGGATCTTTTTGTTACCTGGTCGCACGGTTTGATGCATTGAACGAATTTAATGCAAATTACAGCCGGATTTAGGGTTTTTTTTAATAATAATTTTTCTTTCAGTAGAGTATAAATGTGTTTACTCACAATATTTTATGTTTTGAATCTATTCATAAAATAAACATTTCCAAAATCTCAAGATTCTCGATAAAGTTTTAAAATTAAAAATATATATATTGTTTAAAGATATATATTTAAATAAAAAATTGATAAAAGTATTATATAACGTTTTGAAAATGATGAACTAAGGAATTGTTAGTCAATGCATTTAATTTTAGTAAACATACAGCTAAAGTAGGAACTGGGTATGGAGGCCTTAACATTTAACATCTTTTATCATCAATTTCCCATTTGGTTATGATTTTTCTAAACAATAAAATATCAGTAGATCTGGAGGACTAGAACCTTTCAGCTATATTCGTGTTCTTGTAGTTCATTTTAATGGGTGCTTATTGATCATCAGTCATTTATTTATTTGAATTTTGTAGTTGTGTTCTGTGGAAGCTTTTCAAGTATTCTTAGGCACTATTTCTTGCTGCAGGTTATAGCCGTGAATGGACAGTTTCCTGGTCCTCGAGTCAATGCTACCACAAATTACAACGTTGCTGTCAATGTGTGGAATGATTTGGATGAGAATCTATTATTGACTTGGTAGGTCTACTTGGAAACAAAGTCTCACTGTTCCATCTCCATTTTCTTTGAGTTGTATTTAAAGGAATGGGATTTTGTAAAGGCTTAAAGTGCTGTGTATCATGGCAGGTCTGGGATTCAAATGCGGCGCAATTCGTGGCAAGATGGTGTTCTTGGCACGAACTGTCCAATTCCTCCAAAGTGGAACTGGACATACCAGTTTCAAGTGAAGGATCAGATTGGAAGTTTCTATTACTTTCCATCTCTCAATTTTCAAAAAGCAGCTGGTGGTTTTGGTCCATTTGTTATTAACAACAGGGAGATTATCCCAATTCCTTTTGCCCAACCTGATGGAGATATTTTCATTATGATTGGTGATTGGTATGCACGCAACCACACGGTTAGTTTCTGAATTGTGGTGTGTAATTATTTCTTTTTATATCAATCAATTTCTTACTGTTTTTTCTTTTTTTTTTTTTAATTTTTAATTCAGGCACTCAGGGCAGATCTGGATGCTGGAAAGGATCTTGGAATTCCAGATGGAGTTCTCATGAATGGCAAGGGGCCTTACCAGTACAACAAAACTCTTGTGCCCGCCGGCATACAATATGAAACCATTCAAGTTCATCCAGGTATGGTTCCTCTGTTCGAACTGCTGATGTAATCCAGGTATTATATCAGATCGTTTCTTCCATGGATGATACTCTGCTATTCCTTGAGATCAGTTTAATTTCATCTATTACCAGAAGTATCATGTTTTCTTTTGATCGTTCAGAAAAAGACTATATTTCATCCTGGTGGCATGTTGATGAATGATTAGATAAAAAATAGGGTCACATCTGTTAATGGAATGGGTCCATTGAGAAGTGTAGGATTACACTTACCTCAAACCCACCAATTTGGATTATAAACCCACAAAGTCTTACATTTGTGATTTCTTATCTTAAAAGTTAGAACCCCAGGATAGATGATGCAGAACAGGACGTTCTTTACAACATACTCCCATTTTCGGACATTACTTTGAATAGTTTGGACTCATAACTAATGTTTATCATATGTTTTCAGGCAAAACTTATCGACTTCGAGTGCACAATGTTGGGATCTCTACCAGTTTGAATTTTCGAATCCAGGGTCATAATATGCTTCTGGTAGAAACAGAGGGACATTATACGGTGATGCAAAATTACACAGACTTCGATATTCATGTTGGGCAGTCCTACTCTTTTTTGGTAACCATGGACCAGAATGCCAGTACCGATTACTACATTGTTGCAAGTGCTAGGTTTGTAAACGCATCACTTTGGGAAAAAGTTACTGGTGTTGCCATTTTACACTATTCCAATTCTAAAGGACCAGCAACTGGTCCACTCCCAGATCCTCCAAATGATTTTTATGACAGGGAGAGGTCAATGAACCAGGCCAGGAGTGTAAGGTTCGTTCTTATAGTAGTATCTCTATTTGTATTCTTTATTTCTAGTGATAATGACAGAATTCAAACGTTATCGGTTTTTTTTTTTTTCTAGTTATTATGAATATACTGTTAGCTTCAATATTGTTTTGATTCTTTCAATACTGCGGTGCATTATATTATGACCAATCGATATTGGGTCATTTCATCTTCTTGTTATCCAATGATAATTACGGATCAAGATTGGGTTGTTTCAGAGTTCAAACAGTATAAACTTTTTTTCTCCATATTATGAATGTTTTCAATTCACTTGGGAACTGGTAATTCCTTGTGATTAATGTCTATATTTGATGGATTAGGCAAAATGTATCTGCTAGTGGCGCCCGTCCCAATCCGCAGGGATCATTTCATTACGGTCAAATAAATGTCACGCAGACATATTTGTTGAAGAGTGAACCATTGGTGACAATCAACAGAACAGCTCGGGCAACCTTCAATGGTATCTCGTTTGTTCCTCCAAAAACACCAATCAGGCTAGCTGACCAACATAAAGTTAAGGGAGCGTATAAGCTTGATTTTCCCGAGAGGCCATTGAACAGAACTCCACGAGCCGATATATCTATTATAAATGCAACGTATAAAGGCTTTATTGAAGTGATATTTCAGAACAACGATACCATCATCCACAGTATACATATGAATGGCTATTCATTTTTTGTAGCAGGGTGAGATTTTCCTCTCTTTTTCCCACTTATATACATATTTCAGATAAGAAGCTTGCCTCTACTCTATGCATCACTTGCTTTCAAATGTTGATAATTGCAAATGTATCATTATTGCAGCATGGGTTATGGTGATTGGTCAGAAGACAAAAGAGGTAGCTACAATAAGTGGGATGCTATTGCTCGATGCACGACGCAGGTTTGTATTTTCAAGTTGTCATGGAAATATTTGTATGAAACGTGCGTGTAATGATGCTTGTCATGAATGCATGGTAAAGTAAATTGAGTTCTTTATTCTTCTCTGCCTTTGGCTGTACTTCTTTTCATTCTAAAATTTTAATTGAGCTTAGTAATACTAGGTTTACTTATAATACAGCTGCAACAATCTAAGACCTGACCGACTATGAAGATCTGCATTTCTCATATTTTCTGTGATGGATTGTGTGAAGTAAGAACTTTGAAGATTTCATTGAAAAGAATGATACTTTCTGGTAGTATTGCATCAGGATCACTGGTGTCAATTGACATCCTTATAAGCTCTGAGATTCTGATTTGGCATTCATTTGATAAATTCTATTATGTTGCGGTGTTCAGGTCTACCCCGGGGCTTGGACAGCAGTTCTTATTTCTCTTGACAATGTTGGATCTTGGAATCTTAGAGCAGAAAATCTAGACCGATGGTATTTAGGTCAAGAAACATATTTGAGAATTGTCAACCCGGAGGAGAATGGGAAAACCGAGATGGCTCCTCCTTCGAACGTTCTATACTGCGGTGCATTGCAGAATTTGCAGAAGTATGTGACTGAACACTTGACATATCTTTTTGTTCTTTCCTTGTGCATCAGACAAGCACAATCTAATCTAATCCTGCCTTGTTTTTTGGTTCTCAGAGAACAGAAACACAACAATGCTGATTCAATCTTCAAGGGACACTCAAAACTCTTCATCTCTCTTCTGATGGCATTCCTTCATATTGTTTTCATTTGTAGC

mRNA sequence

CTTTCACTGTTTCATCTCTTTCCACTGCTGCTACTGCTTATGCTTCCGTCGCTCTGTTCCGCCGCTGATCCCTACATCTTTTACGAATTTCGCGTCTCTTACATTACTGCCTCTCCCTTGGGCGTTCCTCAACAGGTTATAGCCGTGAATGGACAGTTTCCTGGTCCTCGAGTCAATGCTACCACAAATTACAACGTTGCTGTCAATGTGTGGAATGATTTGGATGAGAATCTATTATTGACTTGGTCTGGGATTCAAATGCGGCGCAATTCGTGGCAAGATGGTGTTCTTGGCACGAACTGTCCAATTCCTCCAAAGTGGAACTGGACATACCAGTTTCAAGTGAAGGATCAGATTGGAAGTTTCTATTACTTTCCATCTCTCAATTTTCAAAAAGCAGCTGGTGGTTTTGGTCCATTTGTTATTAACAACAGGGAGATTATCCCAATTCCTTTTGCCCAACCTGATGGAGATATTTTCATTATGATTGGTGATTGGTATGCACGCAACCACACGGCACTCAGGGCAGATCTGGATGCTGGAAAGGATCTTGGAATTCCAGATGGAGTTCTCATGAATGGCAAGGGGCCTTACCAGTACAACAAAACTCTTGTGCCCGCCGGCATACAATATGAAACCATTCAAGTTCATCCAGGCAAAACTTATCGACTTCGAGTGCACAATGTTGGGATCTCTACCAGTTTGAATTTTCGAATCCAGGGTCATAATATGCTTCTGGTAGAAACAGAGGGACATTATACGGTGATGCAAAATTACACAGACTTCGATATTCATGTTGGGCAGTCCTACTCTTTTTTGGTAACCATGGACCAGAATGCCAGTACCGATTACTACATTGTTGCAAGTGCTAGGTTTGTAAACGCATCACTTTGGGAAAAAGTTACTGGTGTTGCCATTTTACACTATTCCAATTCTAAAGGACCAGCAACTGGTCCACTCCCAGATCCTCCAAATGATTTTTATGACAGGGAGAGGTCAATGAACCAGGCCAGGAGTGTAAGGCAAAATGTATCTGCTAGTGGCGCCCGTCCCAATCCGCAGGGATCATTTCATTACGGTCAAATAAATGTCACGCAGACATATTTGTTGAAGAGTGAACCATTGGTGACAATCAACAGAACAGCTCGGGCAACCTTCAATGGTATCTCGTTTGTTCCTCCAAAAACACCAATCAGGCTAGCTGACCAACATAAAGTTAAGGGAGCGTATAAGCTTGATTTTCCCGAGAGGCCATTGAACAGAACTCCACGAGCCGATATATCTATTATAAATGCAACGTATAAAGGCTTTATTGAAGTGATATTTCAGAACAACGATACCATCATCCACAGTATACATATGAATGGCTATTCATTTTTTGTAGCAGGCATGGGTTATGGTGATTGGTCAGAAGACAAAAGAGGTAGCTACAATAAGTGGGATGCTATTGCTCGATGCACGACGCAGGTCTACCCCGGGGCTTGGACAGCAGTTCTTATTTCTCTTGACAATGTTGGATCTTGGAATCTTAGAGCAGAAAATCTAGACCGATGGTATTTAGGTCAAGAAACATATTTGAGAATTGTCAACCCGGAGGAGAATGGGAAAACCGAGATGGCTCCTCCTTCGAACGTTCTATACTGCGGTGCATTGCAGAATTTGCAGAAAGAACAGAAACACAACAATGCTGATTCAATCTTCAAGGGACACTCAAAACTCTTCATCTCTCTTCTGATGGCATTCCTTCATATTGTTTTCATTTGTAGC

Coding sequence (CDS)

CTTTCACTGTTTCATCTCTTTCCACTGCTGCTACTGCTTATGCTTCCGTCGCTCTGTTCCGCCGCTGATCCCTACATCTTTTACGAATTTCGCGTCTCTTACATTACTGCCTCTCCCTTGGGCGTTCCTCAACAGGTTATAGCCGTGAATGGACAGTTTCCTGGTCCTCGAGTCAATGCTACCACAAATTACAACGTTGCTGTCAATGTGTGGAATGATTTGGATGAGAATCTATTATTGACTTGGTCTGGGATTCAAATGCGGCGCAATTCGTGGCAAGATGGTGTTCTTGGCACGAACTGTCCAATTCCTCCAAAGTGGAACTGGACATACCAGTTTCAAGTGAAGGATCAGATTGGAAGTTTCTATTACTTTCCATCTCTCAATTTTCAAAAAGCAGCTGGTGGTTTTGGTCCATTTGTTATTAACAACAGGGAGATTATCCCAATTCCTTTTGCCCAACCTGATGGAGATATTTTCATTATGATTGGTGATTGGTATGCACGCAACCACACGGCACTCAGGGCAGATCTGGATGCTGGAAAGGATCTTGGAATTCCAGATGGAGTTCTCATGAATGGCAAGGGGCCTTACCAGTACAACAAAACTCTTGTGCCCGCCGGCATACAATATGAAACCATTCAAGTTCATCCAGGCAAAACTTATCGACTTCGAGTGCACAATGTTGGGATCTCTACCAGTTTGAATTTTCGAATCCAGGGTCATAATATGCTTCTGGTAGAAACAGAGGGACATTATACGGTGATGCAAAATTACACAGACTTCGATATTCATGTTGGGCAGTCCTACTCTTTTTTGGTAACCATGGACCAGAATGCCAGTACCGATTACTACATTGTTGCAAGTGCTAGGTTTGTAAACGCATCACTTTGGGAAAAAGTTACTGGTGTTGCCATTTTACACTATTCCAATTCTAAAGGACCAGCAACTGGTCCACTCCCAGATCCTCCAAATGATTTTTATGACAGGGAGAGGTCAATGAACCAGGCCAGGAGTGTAAGGCAAAATGTATCTGCTAGTGGCGCCCGTCCCAATCCGCAGGGATCATTTCATTACGGTCAAATAAATGTCACGCAGACATATTTGTTGAAGAGTGAACCATTGGTGACAATCAACAGAACAGCTCGGGCAACCTTCAATGGTATCTCGTTTGTTCCTCCAAAAACACCAATCAGGCTAGCTGACCAACATAAAGTTAAGGGAGCGTATAAGCTTGATTTTCCCGAGAGGCCATTGAACAGAACTCCACGAGCCGATATATCTATTATAAATGCAACGTATAAAGGCTTTATTGAAGTGATATTTCAGAACAACGATACCATCATCCACAGTATACATATGAATGGCTATTCATTTTTTGTAGCAGGCATGGGTTATGGTGATTGGTCAGAAGACAAAAGAGGTAGCTACAATAAGTGGGATGCTATTGCTCGATGCACGACGCAGGTCTACCCCGGGGCTTGGACAGCAGTTCTTATTTCTCTTGACAATGTTGGATCTTGGAATCTTAGAGCAGAAAATCTAGACCGATGGTATTTAGGTCAAGAAACATATTTGAGAATTGTCAACCCGGAGGAGAATGGGAAAACCGAGATGGCTCCTCCTTCGAACGTTCTATACTGCGGTGCATTGCAGAATTTGCAGAAAGAACAGAAACACAACAATGCTGATTCAATCTTCAAGGGACACTCAAAACTCTTCATCTCTCTTCTGATGGCATTCCTTCATATTGTTTTCATTTGTAGC

Protein sequence

LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS
Homology
BLAST of MS000318 vs. NCBI nr
Match: XP_022140913.1 (monocopper oxidase-like protein SKS1 [Momordica charantia])

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 588/589 (99.83%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 60
           LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA
Sbjct: 9   LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 68

Query: 61  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 120
           TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 69  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 128

Query: 121 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 180
           SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA
Sbjct: 129 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 188

Query: 181 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 240
           GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ
Sbjct: 189 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 248

Query: 241 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 300
           GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK
Sbjct: 249 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 308

Query: 301 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 360
           VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 309 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 368

Query: 361 QINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 420
           QINVTQTYLLK EPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN
Sbjct: 369 QINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 428

Query: 421 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 480
           RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW
Sbjct: 429 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 488

Query: 481 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 540
           DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA
Sbjct: 489 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 548

Query: 541 PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 590
           PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS
Sbjct: 549 PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597

BLAST of MS000318 vs. NCBI nr
Match: XP_038880777.1 (monocopper oxidase-like protein SKS1 [Benincasa hispida])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 537/589 (91.17%), Postives = 562/589 (95.42%), Query Frame = 0

Query: 2   SLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNAT 61
           SLF+LFP   ++ LPSL SAADPY+FY+FRVSYITASPLGVPQQVIAVN QFPGP +NAT
Sbjct: 5   SLFYLFP---IIFLPSLSSAADPYVFYDFRVSYITASPLGVPQQVIAVNEQFPGPPINAT 64

Query: 62  TNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGS 121
           TNYNVAVNVWNDLDENLL+TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGS
Sbjct: 65  TNYNVAVNVWNDLDENLLITWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGS 124

Query: 122 FYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAG 181
           FYYFPSLNFQKA+GGFGP VINNREIIPIPF QPDGDI IMIGDWY RNHTALR DLDAG
Sbjct: 125 FYYFPSLNFQKASGGFGPIVINNREIIPIPFPQPDGDISIMIGDWYIRNHTALRIDLDAG 184

Query: 182 KDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQG 241
           K+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 
Sbjct: 185 KELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQS 244

Query: 242 HNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKV 301
           HNMLL ETEGHYTVMQNY+DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLWEKV
Sbjct: 245 HNMLLAETEGHYTVMQNYSDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNQSLWEKV 304

Query: 302 TGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQ 361
           TGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYGQ
Sbjct: 305 TGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYGQ 364

Query: 362 INVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNR 421
           INVTQTYLLK EPLVTIN+T RATFNGISF+PP+TPIRLADQHKVKGAYKLDFP+RPLNR
Sbjct: 365 INVTQTYLLKGEPLVTINKTTRATFNGISFIPPQTPIRLADQHKVKGAYKLDFPDRPLNR 424

Query: 422 TPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWD 481
           TPRADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKWD
Sbjct: 425 TPRADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKWD 484

Query: 482 AIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAP 541
           AI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEMA 
Sbjct: 485 AITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMAA 544

Query: 542 PSNVLYCGALQNLQKEQ-KHNNADSIFKGHSKLFISLLMAFLHIVFICS 590
           PSNVLYCGAL++LQKEQ  H+NA SI KGHSKLFI+LLMA L++VFI S
Sbjct: 545 PSNVLYCGALRSLQKEQHHHSNAKSILKGHSKLFITLLMALLNLVFIFS 590

BLAST of MS000318 vs. NCBI nr
Match: XP_008439091.1 (PREDICTED: monocopper oxidase-like protein SKS1 [Cucumis melo])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 536/591 (90.69%), Postives = 563/591 (95.26%), Query Frame = 0

Query: 2   SLFHL-FPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 61
           SLFHL FP   ++ LPSLCSAADPY+FY+FR+SYITASPLGVPQQVIAVN QFPGP +NA
Sbjct: 5   SLFHLIFP---IIFLPSLCSAADPYVFYDFRLSYITASPLGVPQQVIAVNEQFPGPPINA 64

Query: 62  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 121
           TTNYNV VNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 65  TTNYNVVVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 124

Query: 122 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 181
           SFYYFPSLNFQ+A+GGFGP VINNREIIPIPF +PDG+I IMIGDWY RNH ALRADLDA
Sbjct: 125 SFYYFPSLNFQRASGGFGPIVINNREIIPIPFPEPDGEISIMIGDWYTRNHKALRADLDA 184

Query: 182 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 241
           GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFRIQ
Sbjct: 185 GKELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFRIQ 244

Query: 242 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 301
            H MLL ETEGHYTVMQN+TDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+K
Sbjct: 245 SHYMLLAETEGHYTVMQNFTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQK 304

Query: 302 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 361
           VTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 305 VTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYG 364

Query: 362 QINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 421
           QINVT+TYLLKSEPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP+RPLN
Sbjct: 365 QINVTRTYLLKSEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPDRPLN 424

Query: 422 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 481
           RTP+ADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKW
Sbjct: 425 RTPKADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKW 484

Query: 482 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 541
           DAI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEMA
Sbjct: 485 DAITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMA 544

Query: 542 PPSNVLYCGALQNLQKEQKH--NNADSIFKGHSKLFISLLMAFLHIVFICS 590
            PSNVLYCGALQ+LQKEQ H  NNA SIFKGHSKLFI+LLMA L++V I S
Sbjct: 545 APSNVLYCGALQSLQKEQHHKSNNAKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MS000318 vs. NCBI nr
Match: XP_011651117.1 (monocopper oxidase-like protein SKS1 [Cucumis sativus] >KGN57269.1 hypothetical protein Csa_010920 [Cucumis sativus])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 532/592 (89.86%), Postives = 558/592 (94.26%), Query Frame = 0

Query: 1   LSLFHLFPLLL--LLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRV 60
           + LF LF  L+  ++ LPSLCSAADPY+ Y+FR+SYITASPLGVPQQVIAVN QFPGP +
Sbjct: 1   MPLFSLFRHLIFPIIFLPSLCSAADPYVSYDFRLSYITASPLGVPQQVIAVNEQFPGPPI 60

Query: 61  NATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQ 120
           NATTNYNV VNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQ
Sbjct: 61  NATTNYNVVVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQ 120

Query: 121 IGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADL 180
           IGSFYYFPSLNFQ+A+GGFGP VINNREII IPF QPDG+I IMIGDWY RNHTALRADL
Sbjct: 121 IGSFYYFPSLNFQRASGGFGPIVINNREIISIPFPQPDGEISIMIGDWYTRNHTALRADL 180

Query: 181 DAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFR 240
           DAGK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFR
Sbjct: 181 DAGKELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFR 240

Query: 241 IQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLW 300
           IQ HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW
Sbjct: 241 IQSHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLW 300

Query: 301 EKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFH 360
           +KVTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFH
Sbjct: 301 QKVTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFH 360

Query: 361 YGQINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERP 420
           YGQINVT TYLLKSEPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP  P
Sbjct: 361 YGQINVTHTYLLKSEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPHTP 420

Query: 421 LNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYN 480
           LNRTP+ADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYN
Sbjct: 421 LNRTPKADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYN 480

Query: 481 KWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTE 540
           KWDAI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTE
Sbjct: 481 KWDAITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTE 540

Query: 541 MAPPSNVLYCGALQNLQKEQKH-NNADSIFKGHSKLFISLLMAFLHIVFICS 590
           M  PSNVLYCGALQ+LQKEQ H +N  SIFKGHSKLFI+LLMA L++V I S
Sbjct: 541 MEAPSNVLYCGALQSLQKEQHHKSNGKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MS000318 vs. NCBI nr
Match: KAG6576799.1 (Monocopper oxidase-like protein SKS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 524/589 (88.96%), Postives = 558/589 (94.74%), Query Frame = 0

Query: 1   LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 60
           LSLF+L P   ++ LPS CSAADPY  YEFRVSYITASPLGVPQ+VIAVNG FPGP +N 
Sbjct: 8   LSLFYLLP---IIFLPSFCSAADPYASYEFRVSYITASPLGVPQKVIAVNGNFPGPPINV 67

Query: 61  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 120
           TTNYNVAVNVWNDLDENLL+TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 68  TTNYNVAVNVWNDLDENLLMTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 127

Query: 121 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 180
           SF+YFPS+NFQKA+GGFGPFVINNR+IIPIPFAQP+GDIFIMIGDW+ R+HTALRADL +
Sbjct: 128 SFFYFPSINFQKASGGFGPFVINNRDIIPIPFAQPEGDIFIMIGDWFTRDHTALRADLSS 187

Query: 181 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 240
           GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETI+V PGKTYRLRVHNVGISTSLNFRIQ
Sbjct: 188 GKELGIPDGVLINGKGPYQYNATLVPAGIQYETIRVDPGKTYRLRVHNVGISTSLNFRIQ 247

Query: 241 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 300
            HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+K
Sbjct: 248 SHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQK 307

Query: 301 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 360
           VTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 308 VTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYG 367

Query: 361 QINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 420
           QINVTQTYLLK EPLVTIN+TARATFNGISF+PPKTPIRLADQHKVKGAYKLDFP+ PLN
Sbjct: 368 QINVTQTYLLKGEPLVTINKTARATFNGISFIPPKTPIRLADQHKVKGAYKLDFPDMPLN 427

Query: 421 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 480
           RTPRADISII+A YKGFIEVIFQNND+IIHS+H++GYSFFV GMGYGDWSEDKRGSYNKW
Sbjct: 428 RTPRADISIIDAAYKGFIEVIFQNNDSIIHSVHLDGYSFFVVGMGYGDWSEDKRGSYNKW 487

Query: 481 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 540
           DAI R T+QVYPGAWTAVLISLDNVG WNLRAENLDRWYLGQETYLRIVNPEENGKTEMA
Sbjct: 488 DAITRSTSQVYPGAWTAVLISLDNVGVWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 547

Query: 541 PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 590
            PSNVLYCGALQ+LQKEQ H NA+SIFKGHSKLFI+LLMA L++ F+ S
Sbjct: 548 APSNVLYCGALQSLQKEQHHGNANSIFKGHSKLFITLLMALLNVAFLLS 593

BLAST of MS000318 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 8.9e-259
Identity = 422/578 (73.01%), Postives = 488/578 (84.43%), Query Frame = 0

Query: 9   LLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAV 68
           LL   +L ++  AADP++ Y+FRVSY+TASPLGVPQQVIAVNGQFPGP +NATTNYNV V
Sbjct: 12  LLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVV 71

Query: 69  NVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSL 128
           NV+N LDE LLLTW GIQMRRNSWQDGVLGTNCPIPP+WN+TYQFQVKDQIGSF+Y PSL
Sbjct: 72  NVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSL 131

Query: 129 NFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPD 188
           NFQ+A+GGFGP VINNR+IIPIPF QPDG++  +IGDWY ++H ALR  LD+GK+LG+PD
Sbjct: 132 NFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPD 191

Query: 189 GVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVE 248
           GVL+NGKGPY+YN + VP GI Y T  V PGKTYR+RVHNVGISTSLNFRIQ H++LLVE
Sbjct: 192 GVLINGKGPYKYNSS-VPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVE 251

Query: 249 TEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILH 308
           TEGHYT   N+TDFD+HVGQSYSFLVTMDQ+A++DYYIVASARFVN ++W++VTGVAILH
Sbjct: 252 TEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILH 311

Query: 309 YSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTY 368
           YSNSKGP +GPLP P  D      +M+Q +++RQN SASGARPNPQGSFHYGQIN+T TY
Sbjct: 312 YSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTY 371

Query: 369 LLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADIS 428
           +L+S P   IN   RAT NGISFV P TP+RLAD++KVKGAYKLDFP+RP NR  R D S
Sbjct: 372 ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKLDFPDRPFNRPLRLDRS 431

Query: 429 IINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTT 488
           +INATYKGFI+V+FQNNDT I S H++GYSFFV GM +G WSEDK+GSYN WDAI+R T 
Sbjct: 432 MINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTI 491

Query: 489 QVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYC 548
           +VYPG WTAVLISLDNVG WN+R ENLDRWYLG+ETY+RI NPEE+GKTEM PP NVLYC
Sbjct: 492 EVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYC 551

Query: 549 GALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVF 587
           GAL+NLQKEQ H+ A SI  GH KL   LLM  L  VF
Sbjct: 552 GALKNLQKEQHHSAATSILNGHLKLM--LLMVLLASVF 586

BLAST of MS000318 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 3.2e-248
Identity = 398/564 (70.57%), Postives = 471/564 (83.51%), Query Frame = 0

Query: 21  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 80
           A DPY+ Y+F +SYITASPLGVPQQVIAVNG+FPGP +NATTNYNV VNV N LDE LLL
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 81  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 140
           TW G+QMRRNSWQDGVLGTNCPIPP WN+TY FQ+KDQIGS++Y PSLNFQ+A+GGFG  
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 141 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 200
           +INNR+++PIPF +PDG+I  +IGDWY +NHTALR  LD+GK+LG+PDGVL+NGKGP++Y
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 201 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 260
           N + VP GI++ET+ V PGKTYR+RVHNVGISTSLNFRIQ H +LL+ETEG YT   N+T
Sbjct: 203 NSS-VPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFT 262

Query: 261 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 320
           DFD+HVGQSYSFLVTMDQNA++DYYIVASARFVN ++W++VTGV ILHYSNSKGPA+GPL
Sbjct: 263 DFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPL 322

Query: 321 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKSEPLVTINR 380
           P    D      +MNQ R+++QN SASGARPNPQGSFHYGQIN+T+TY+L+S P   IN 
Sbjct: 323 PVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKING 382

Query: 381 TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN-RTPRADISIINATYKGFIE 440
             RAT NGISFV P TP+RLAD HKVKG Y LDFP+RPL+ + PR   SIINATYKGFI+
Sbjct: 383 KLRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSIINATYKGFIQ 442

Query: 441 VIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVL 500
           VIFQNNDT I S H++GY+F+V  M +G WSED+  SYN WDA+AR T +VYPGAWTAVL
Sbjct: 443 VIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVL 502

Query: 501 ISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQKEQK 560
           ISLDNVG WN+R ENLDRWYLGQETY+RI+NPEENG TEM PP NV+YCGALQ +QKEQ 
Sbjct: 503 ISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAMQKEQH 562

Query: 561 HNNA-DSIFKGHSKLFISLLMAFL 583
           H++A  S+  G   L  S++M  L
Sbjct: 563 HSSATKSMTNGQLILIFSMMMVLL 585

BLAST of MS000318 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 4.0e-243
Identity = 406/584 (69.52%), Postives = 466/584 (79.79%), Query Frame = 0

Query: 6   LFPLLLLLMLP--SLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTN 65
           LF +LLL+     S C AADPY FY F VSYITASPLGVPQQVIA+NG+FPGP +N TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 66  YNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFY 125
            N+ VNV N LDE LLL W+GIQ RR SWQDGVLGTNCPIPPKWNWTY+FQVKDQIGSF+
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 126 YFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKD 185
           YFPSL+FQ+A+GGFG FV+N R IIP+PF+ PDGDI + IGDWY RNHTALR  LD GKD
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 186 LGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHN 245
           LG+PDGVL+NGKGPY+YN TLV  GI +ETI VHPGKTYRLRV NVGISTSLNFRIQGHN
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 246 MLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTG 305
           ++L E+EG YTV QNYT  DIHVGQSYSFLVTMDQNAS+DYYIVASAR VN ++W +VTG
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 306 VAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQIN 365
           V IL Y+NSKG A G LP  P D +D+  SMNQARS+R NVSASGARPNPQGSF YG IN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 366 VTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTP 425
           VT  Y+L++ P VTI+   R T NGISF  P TPIRLAD+ KVK  YKLDFP+RPL    
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLDFPKRPLTGPA 422

Query: 426 RADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAI 485
           +   SIIN TY+GF+EV+ QNNDT + S HM+GY+FFV GM YG+W+E+ RG+YNKWD I
Sbjct: 423 KVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGI 482

Query: 486 ARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPS 545
           AR T QVYPGAW+A+LISLDN G+WNLR ENLD WYLGQETY+R+VNP+EN KTE   P 
Sbjct: 483 ARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPD 542

Query: 546 NVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFI 588
           NVLYCGAL  LQK QK +++ S   G    F SL M  + +V +
Sbjct: 543 NVLYCGALSKLQKPQKVSSSASKSIG----FTSLSMVVMALVMM 582

BLAST of MS000318 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.3e-140
Identity = 242/541 (44.73%), Postives = 350/541 (64.70%), Query Frame = 0

Query: 21  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 80
           A DPY  + + V+Y TASPLGVPQQVI +NGQFPGP +N+T+N NV +NV+N+LDE  LL
Sbjct: 23  AEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIINVFNNLDEPFLL 82

Query: 81  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 140
           TW+GIQ R+N WQDG  GT CPI P  N+TY FQ KDQIGS++Y+P+    +AAGG+G  
Sbjct: 83  TWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPTTGMHRAAGGYGGL 142

Query: 141 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 200
            +N+R +IP+P+A P+ D  ++IGDWY ++HT L+  LD G+ +G PDG+++NGK     
Sbjct: 143 RVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGKSG--- 202

Query: 201 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 260
                  G       + PGKTYR+R+ NVG+ TS+NFRIQ H M LVE EG + +  +Y 
Sbjct: 203 ----KGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHVLQNDYD 262

Query: 261 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 320
             D+HVGQ +  +VT +Q    DYY+VAS+RF+       +T   +L Y   KGPA+  L
Sbjct: 263 SLDVHVGQCFGTIVTANQEPK-DYYMVASSRFLKT----VITTTGLLRYEGGKGPASSQL 322

Query: 321 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKSEPLVTINR 380
           P  P  +     S+NQ RS R N++AS ARPNPQGS+HYG+IN+T+T  L +     ++ 
Sbjct: 323 PAGPVGW---AWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQ-GKVDG 382

Query: 381 TARATFNGISFVPPKTPIRLADQHKVKG-AYKLDF----PERPLNRTPRADISIINATYK 440
             R   NG+S   P+TP++LA+   +    +K D     P     +  + + +++N T++
Sbjct: 383 KLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHR 442

Query: 441 GFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAW 500
            F+EV+F+N++  + S H++GYSFF   +  G W+ +KR +YN  DA++R T QVYP  W
Sbjct: 443 TFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 502

Query: 501 TAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQ 557
            A+L++ DN G WN+R+EN +R YLGQ+ Y  +++PE++ + E   P   L CG ++N  
Sbjct: 503 AAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNTP 547

BLAST of MS000318 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 4.7e-135
Identity = 245/552 (44.38%), Postives = 346/552 (62.68%), Query Frame = 0

Query: 11  LLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNV 70
           LLL L     A DPY+++ + V+Y T +PLGVPQQ I +NGQFPGPR+N T+N N+ VNV
Sbjct: 11  LLLCLSVGVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVVNV 70

Query: 71  WNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNF 130
           +N+LDE  L TW+G+Q R+NSWQDG  GT CPI P  N+TY+FQVKDQIGS+ YFP+   
Sbjct: 71  FNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTTAL 130

Query: 131 QKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGV 190
            +AAGG+G   +++R +IP+PF  P  +  + +GDWY + H  L+  LD G+ +G PDG+
Sbjct: 131 HRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGI 190

Query: 191 LMNGKGPYQYNKTLVPAGIQYETI-QVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVET 250
           ++NGK            G   E +  +  GKTYR R  N+G+ +S+N R QGH M LVE 
Sbjct: 191 IINGKS--------AKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVEL 250

Query: 251 EGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHY 310
           EG +TV   Y   D+HVGQ  S LVT DQ    DYY+V S+RF+  +L    + VAI+ Y
Sbjct: 251 EGSHTVQNIYDSLDLHVGQCLSVLVTADQEPK-DYYLVVSSRFLKQAL----SSVAIIRY 310

Query: 311 SNSKGPATGPLPDPPNDFYDR-ERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTY 370
           +N KGPA+  LP PP +  +    SMNQ RS R N++AS ARPNPQGS+HYGQIN+T+T 
Sbjct: 311 ANGKGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTI 370

Query: 371 LLKSEPLVTINRTARATFNGISFVPPKTPIRLADQH-KVKGAYKLDF-----PERPLNRT 430
            + +  +  +    R   NGIS    +TP++L +       A+K D      P  P   T
Sbjct: 371 KIFNS-MSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLT 430

Query: 431 PRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDA 490
              ++   NATY+ F+E+IF+N++  I + H++GYSFF   +  G WS +KR +YN  D 
Sbjct: 431 IATNVK--NATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDG 490

Query: 491 IARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPP 550
           ++R   QVYP +W A++++ DN G WNLR+E  ++ YLG++ Y  +++P  + + E   P
Sbjct: 491 LSRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIP 546

Query: 551 SNVLYCGALQNL 555
            N   CG ++ L
Sbjct: 551 DNHPLCGIVKGL 546

BLAST of MS000318 vs. ExPASy TrEMBL
Match: A0A6J1CJ41 (monocopper oxidase-like protein SKS1 OS=Momordica charantia OX=3673 GN=LOC111011462 PE=3 SV=1)

HSP 1 Score: 1221.1 bits (3158), Expect = 0.0e+00
Identity = 588/589 (99.83%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 60
           LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA
Sbjct: 9   LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 68

Query: 61  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 120
           TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 69  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 128

Query: 121 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 180
           SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA
Sbjct: 129 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 188

Query: 181 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 240
           GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ
Sbjct: 189 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 248

Query: 241 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 300
           GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK
Sbjct: 249 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 308

Query: 301 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 360
           VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 309 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 368

Query: 361 QINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 420
           QINVTQTYLLK EPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN
Sbjct: 369 QINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 428

Query: 421 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 480
           RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW
Sbjct: 429 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 488

Query: 481 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 540
           DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA
Sbjct: 489 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 548

Query: 541 PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 590
           PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS
Sbjct: 549 PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597

BLAST of MS000318 vs. ExPASy TrEMBL
Match: A0A1S3AXI5 (monocopper oxidase-like protein SKS1 OS=Cucumis melo OX=3656 GN=LOC103483983 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 536/591 (90.69%), Postives = 563/591 (95.26%), Query Frame = 0

Query: 2   SLFHL-FPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 61
           SLFHL FP   ++ LPSLCSAADPY+FY+FR+SYITASPLGVPQQVIAVN QFPGP +NA
Sbjct: 5   SLFHLIFP---IIFLPSLCSAADPYVFYDFRLSYITASPLGVPQQVIAVNEQFPGPPINA 64

Query: 62  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 121
           TTNYNV VNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 65  TTNYNVVVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 124

Query: 122 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 181
           SFYYFPSLNFQ+A+GGFGP VINNREIIPIPF +PDG+I IMIGDWY RNH ALRADLDA
Sbjct: 125 SFYYFPSLNFQRASGGFGPIVINNREIIPIPFPEPDGEISIMIGDWYTRNHKALRADLDA 184

Query: 182 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 241
           GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFRIQ
Sbjct: 185 GKELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFRIQ 244

Query: 242 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 301
            H MLL ETEGHYTVMQN+TDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+K
Sbjct: 245 SHYMLLAETEGHYTVMQNFTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQK 304

Query: 302 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 361
           VTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 305 VTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYG 364

Query: 362 QINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 421
           QINVT+TYLLKSEPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP+RPLN
Sbjct: 365 QINVTRTYLLKSEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPDRPLN 424

Query: 422 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 481
           RTP+ADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKW
Sbjct: 425 RTPKADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKW 484

Query: 482 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 541
           DAI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEMA
Sbjct: 485 DAITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMA 544

Query: 542 PPSNVLYCGALQNLQKEQKH--NNADSIFKGHSKLFISLLMAFLHIVFICS 590
            PSNVLYCGALQ+LQKEQ H  NNA SIFKGHSKLFI+LLMA L++V I S
Sbjct: 545 APSNVLYCGALQSLQKEQHHKSNNAKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MS000318 vs. ExPASy TrEMBL
Match: A0A0A0L8C4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G175700 PE=3 SV=1)

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 532/592 (89.86%), Postives = 558/592 (94.26%), Query Frame = 0

Query: 1   LSLFHLFPLLL--LLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRV 60
           + LF LF  L+  ++ LPSLCSAADPY+ Y+FR+SYITASPLGVPQQVIAVN QFPGP +
Sbjct: 1   MPLFSLFRHLIFPIIFLPSLCSAADPYVSYDFRLSYITASPLGVPQQVIAVNEQFPGPPI 60

Query: 61  NATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQ 120
           NATTNYNV VNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQ
Sbjct: 61  NATTNYNVVVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQ 120

Query: 121 IGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADL 180
           IGSFYYFPSLNFQ+A+GGFGP VINNREII IPF QPDG+I IMIGDWY RNHTALRADL
Sbjct: 121 IGSFYYFPSLNFQRASGGFGPIVINNREIISIPFPQPDGEISIMIGDWYTRNHTALRADL 180

Query: 181 DAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFR 240
           DAGK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFR
Sbjct: 181 DAGKELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFR 240

Query: 241 IQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLW 300
           IQ HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW
Sbjct: 241 IQSHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLW 300

Query: 301 EKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFH 360
           +KVTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFH
Sbjct: 301 QKVTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFH 360

Query: 361 YGQINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERP 420
           YGQINVT TYLLKSEPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP  P
Sbjct: 361 YGQINVTHTYLLKSEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPHTP 420

Query: 421 LNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYN 480
           LNRTP+ADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYN
Sbjct: 421 LNRTPKADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYN 480

Query: 481 KWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTE 540
           KWDAI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTE
Sbjct: 481 KWDAITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTE 540

Query: 541 MAPPSNVLYCGALQNLQKEQKH-NNADSIFKGHSKLFISLLMAFLHIVFICS 590
           M  PSNVLYCGALQ+LQKEQ H +N  SIFKGHSKLFI+LLMA L++V I S
Sbjct: 541 MEAPSNVLYCGALQSLQKEQHHKSNGKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MS000318 vs. ExPASy TrEMBL
Match: A0A6J1E5V7 (monocopper oxidase-like protein SKS1 OS=Cucurbita moschata OX=3662 GN=LOC111430865 PE=3 SV=1)

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 524/589 (88.96%), Postives = 558/589 (94.74%), Query Frame = 0

Query: 1   LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 60
           LSLF+L P   ++ LPS CSAADPY  YEFRVSYITASPLGVPQ+VIAVNG FPGP +N 
Sbjct: 8   LSLFYLLP---IIFLPSFCSAADPYASYEFRVSYITASPLGVPQKVIAVNGNFPGPPINV 67

Query: 61  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 120
           TTNYNVAVNVWNDLDENLL+TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 68  TTNYNVAVNVWNDLDENLLMTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 127

Query: 121 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 180
           SF+YFPS+NFQKA+GGFGPFVINNR+IIPIPFAQP+GDIFIMIGDW+ R+HTALRADL +
Sbjct: 128 SFFYFPSINFQKASGGFGPFVINNRDIIPIPFAQPEGDIFIMIGDWFTRDHTALRADLSS 187

Query: 181 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 240
           GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETI+V PGKTYRLRVHNVGISTSLNFRIQ
Sbjct: 188 GKELGIPDGVLINGKGPYQYNATLVPAGIQYETIRVDPGKTYRLRVHNVGISTSLNFRIQ 247

Query: 241 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 300
            HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+K
Sbjct: 248 SHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQK 307

Query: 301 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 360
           VTGVAIL YSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 308 VTGVAILQYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYG 367

Query: 361 QINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 420
           QINVTQTYLLKSEPLVTIN+TARATFNGISF+PPKTPIRLADQHKVKGAYKLDFP+ PLN
Sbjct: 368 QINVTQTYLLKSEPLVTINKTARATFNGISFIPPKTPIRLADQHKVKGAYKLDFPDMPLN 427

Query: 421 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 480
           RTPRADISII+A YKGFIEVIFQNND+IIHS+H++GYSFFV GMGYGDWSEDKRGSYNKW
Sbjct: 428 RTPRADISIIDAAYKGFIEVIFQNNDSIIHSVHLDGYSFFVVGMGYGDWSEDKRGSYNKW 487

Query: 481 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 540
           DAI R T+QVYPGAWTAVLISLDNVG WNLRAENLDRWYLGQETYLRIVNPEENGKTEMA
Sbjct: 488 DAITRSTSQVYPGAWTAVLISLDNVGVWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 547

Query: 541 PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 590
            PSNVLYCGALQ+LQKEQ H NA+SIFKGHSKLFI+LLMA L++ F+ S
Sbjct: 548 APSNVLYCGALQSLQKEQHHGNANSIFKGHSKLFITLLMALLNVAFLLS 593

BLAST of MS000318 vs. ExPASy TrEMBL
Match: A0A6J1J899 (monocopper oxidase-like protein SKS1 OS=Cucurbita maxima OX=3661 GN=LOC111483473 PE=3 SV=1)

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 522/589 (88.62%), Postives = 556/589 (94.40%), Query Frame = 0

Query: 1   LSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 60
           LSLF+L P   ++ LPS CSAADPY  YEFRVSYITASPLGVPQ+VIAVNG FPGP +N 
Sbjct: 8   LSLFYLLP---IIFLPSFCSAADPYASYEFRVSYITASPLGVPQKVIAVNGNFPGPPINV 67

Query: 61  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 120
           TTNYNVAVNVWNDLDENLL+TWSGIQMRRNSW DGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 68  TTNYNVAVNVWNDLDENLLMTWSGIQMRRNSWHDGVLGTNCPIPPKWNWTYQFQVKDQIG 127

Query: 121 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 180
           SF+YFPS+NFQKA+GGFGPFVINNR+IIPIPFAQP+GDIFIMIGDW+ R+H ALRADL A
Sbjct: 128 SFFYFPSINFQKASGGFGPFVINNRDIIPIPFAQPEGDIFIMIGDWFTRDHAALRADLSA 187

Query: 181 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 240
           GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETI+VHPGKTYRLRVHNVGISTSLNFRIQ
Sbjct: 188 GKELGIPDGVLINGKGPYQYNATLVPAGIQYETIRVHPGKTYRLRVHNVGISTSLNFRIQ 247

Query: 241 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 300
            HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYY+VASARFVN SLW+K
Sbjct: 248 SHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYVVASARFVNESLWQK 307

Query: 301 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 360
           VTGVAIL YSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 308 VTGVAILQYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYG 367

Query: 361 QINVTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 420
           QINVTQTYLLKSEPLVTIN+T RATFNGISF+PPKTPIRLADQHKVKGAYKLDFP+RPLN
Sbjct: 368 QINVTQTYLLKSEPLVTINKTTRATFNGISFIPPKTPIRLADQHKVKGAYKLDFPDRPLN 427

Query: 421 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 480
           RTPRADISII+A YKGFIEVIFQNND+IIHS+H++GYSFFV GMGYGDWSED RGSYNKW
Sbjct: 428 RTPRADISIIDAAYKGFIEVIFQNNDSIIHSVHLDGYSFFVVGMGYGDWSEDLRGSYNKW 487

Query: 481 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 540
           DAI R T+QVYPGAWTAVLISLDNVG WNLRAENLDRWYLGQETYLRIVNPEENGKTEMA
Sbjct: 488 DAITRSTSQVYPGAWTAVLISLDNVGVWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 547

Query: 541 PPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 590
            PSNVLYCGALQ+LQKEQ H NA+SIFKGHSKLFI+LLMA L++ F+ S
Sbjct: 548 APSNVLYCGALQSLQKEQHHGNANSIFKGHSKLFITLLMALLNVAFLHS 593

BLAST of MS000318 vs. TAIR 10
Match: AT4G25240.1 (SKU5 similar 1 )

HSP 1 Score: 894.0 bits (2309), Expect = 6.3e-260
Identity = 422/578 (73.01%), Postives = 488/578 (84.43%), Query Frame = 0

Query: 9   LLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAV 68
           LL   +L ++  AADP++ Y+FRVSY+TASPLGVPQQVIAVNGQFPGP +NATTNYNV V
Sbjct: 12  LLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVV 71

Query: 69  NVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSL 128
           NV+N LDE LLLTW GIQMRRNSWQDGVLGTNCPIPP+WN+TYQFQVKDQIGSF+Y PSL
Sbjct: 72  NVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSL 131

Query: 129 NFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPD 188
           NFQ+A+GGFGP VINNR+IIPIPF QPDG++  +IGDWY ++H ALR  LD+GK+LG+PD
Sbjct: 132 NFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPD 191

Query: 189 GVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVE 248
           GVL+NGKGPY+YN + VP GI Y T  V PGKTYR+RVHNVGISTSLNFRIQ H++LLVE
Sbjct: 192 GVLINGKGPYKYNSS-VPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVE 251

Query: 249 TEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILH 308
           TEGHYT   N+TDFD+HVGQSYSFLVTMDQ+A++DYYIVASARFVN ++W++VTGVAILH
Sbjct: 252 TEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILH 311

Query: 309 YSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTY 368
           YSNSKGP +GPLP P  D      +M+Q +++RQN SASGARPNPQGSFHYGQIN+T TY
Sbjct: 312 YSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTY 371

Query: 369 LLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADIS 428
           +L+S P   IN   RAT NGISFV P TP+RLAD++KVKGAYKLDFP+RP NR  R D S
Sbjct: 372 ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKLDFPDRPFNRPLRLDRS 431

Query: 429 IINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTT 488
           +INATYKGFI+V+FQNNDT I S H++GYSFFV GM +G WSEDK+GSYN WDAI+R T 
Sbjct: 432 MINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTI 491

Query: 489 QVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYC 548
           +VYPG WTAVLISLDNVG WN+R ENLDRWYLG+ETY+RI NPEE+GKTEM PP NVLYC
Sbjct: 492 EVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYC 551

Query: 549 GALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVF 587
           GAL+NLQKEQ H+ A SI  GH KL   LLM  L  VF
Sbjct: 552 GALKNLQKEQHHSAATSILNGHLKLM--LLMVLLASVF 586

BLAST of MS000318 vs. TAIR 10
Match: AT5G51480.1 (SKU5 similar 2 )

HSP 1 Score: 859.0 bits (2218), Expect = 2.2e-249
Identity = 398/564 (70.57%), Postives = 471/564 (83.51%), Query Frame = 0

Query: 21  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 80
           A DPY+ Y+F +SYITASPLGVPQQVIAVNG+FPGP +NATTNYNV VNV N LDE LLL
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 81  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 140
           TW G+QMRRNSWQDGVLGTNCPIPP WN+TY FQ+KDQIGS++Y PSLNFQ+A+GGFG  
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 141 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 200
           +INNR+++PIPF +PDG+I  +IGDWY +NHTALR  LD+GK+LG+PDGVL+NGKGP++Y
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 201 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 260
           N + VP GI++ET+ V PGKTYR+RVHNVGISTSLNFRIQ H +LL+ETEG YT   N+T
Sbjct: 203 NSS-VPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFT 262

Query: 261 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 320
           DFD+HVGQSYSFLVTMDQNA++DYYIVASARFVN ++W++VTGV ILHYSNSKGPA+GPL
Sbjct: 263 DFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPL 322

Query: 321 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKSEPLVTINR 380
           P    D      +MNQ R+++QN SASGARPNPQGSFHYGQIN+T+TY+L+S P   IN 
Sbjct: 323 PVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKING 382

Query: 381 TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN-RTPRADISIINATYKGFIE 440
             RAT NGISFV P TP+RLAD HKVKG Y LDFP+RPL+ + PR   SIINATYKGFI+
Sbjct: 383 KLRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSIINATYKGFIQ 442

Query: 441 VIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVL 500
           VIFQNNDT I S H++GY+F+V  M +G WSED+  SYN WDA+AR T +VYPGAWTAVL
Sbjct: 443 VIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVL 502

Query: 501 ISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQKEQK 560
           ISLDNVG WN+R ENLDRWYLGQETY+RI+NPEENG TEM PP NV+YCGALQ +QKEQ 
Sbjct: 503 ISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAMQKEQH 562

Query: 561 HNNA-DSIFKGHSKLFISLLMAFL 583
           H++A  S+  G   L  S++M  L
Sbjct: 563 HSSATKSMTNGQLILIFSMMMVLL 585

BLAST of MS000318 vs. TAIR 10
Match: AT4G12420.1 (Cupredoxin superfamily protein )

HSP 1 Score: 842.0 bits (2174), Expect = 2.8e-244
Identity = 406/584 (69.52%), Postives = 466/584 (79.79%), Query Frame = 0

Query: 6   LFPLLLLLMLP--SLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTN 65
           LF +LLL+     S C AADPY FY F VSYITASPLGVPQQVIA+NG+FPGP +N TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 66  YNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFY 125
            N+ VNV N LDE LLL W+GIQ RR SWQDGVLGTNCPIPPKWNWTY+FQVKDQIGSF+
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 126 YFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKD 185
           YFPSL+FQ+A+GGFG FV+N R IIP+PF+ PDGDI + IGDWY RNHTALR  LD GKD
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 186 LGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHN 245
           LG+PDGVL+NGKGPY+YN TLV  GI +ETI VHPGKTYRLRV NVGISTSLNFRIQGHN
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 246 MLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTG 305
           ++L E+EG YTV QNYT  DIHVGQSYSFLVTMDQNAS+DYYIVASAR VN ++W +VTG
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 306 VAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQIN 365
           V IL Y+NSKG A G LP  P D +D+  SMNQARS+R NVSASGARPNPQGSF YG IN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 366 VTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTP 425
           VT  Y+L++ P VTI+   R T NGISF  P TPIRLAD+ KVK  YKLDFP+RPL    
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLDFPKRPLTGPA 422

Query: 426 RADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAI 485
           +   SIIN TY+GF+EV+ QNNDT + S HM+GY+FFV GM YG+W+E+ RG+YNKWD I
Sbjct: 423 KVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGI 482

Query: 486 ARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPS 545
           AR T QVYPGAW+A+LISLDN G+WNLR ENLD WYLGQETY+R+VNP+EN KTE   P 
Sbjct: 483 ARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPD 542

Query: 546 NVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFI 588
           NVLYCGAL  LQK QK +++ S   G    F SL M  + +V +
Sbjct: 543 NVLYCGALSKLQKPQKVSSSASKSIG----FTSLSMVVMALVMM 582

BLAST of MS000318 vs. TAIR 10
Match: AT4G12420.2 (Cupredoxin superfamily protein )

HSP 1 Score: 842.0 bits (2174), Expect = 2.8e-244
Identity = 406/584 (69.52%), Postives = 466/584 (79.79%), Query Frame = 0

Query: 6   LFPLLLLLMLP--SLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTN 65
           LF +LLL+     S C AADPY FY F VSYITASPLGVPQQVIA+NG+FPGP +N TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 66  YNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFY 125
            N+ VNV N LDE LLL W+GIQ RR SWQDGVLGTNCPIPPKWNWTY+FQVKDQIGSF+
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 126 YFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKD 185
           YFPSL+FQ+A+GGFG FV+N R IIP+PF+ PDGDI + IGDWY RNHTALR  LD GKD
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 186 LGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHN 245
           LG+PDGVL+NGKGPY+YN TLV  GI +ETI VHPGKTYRLRV NVGISTSLNFRIQGHN
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 246 MLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTG 305
           ++L E+EG YTV QNYT  DIHVGQSYSFLVTMDQNAS+DYYIVASAR VN ++W +VTG
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 306 VAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQIN 365
           V IL Y+NSKG A G LP  P D +D+  SMNQARS+R NVSASGARPNPQGSF YG IN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 366 VTQTYLLKSEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTP 425
           VT  Y+L++ P VTI+   R T NGISF  P TPIRLAD+ KVK  YKLDFP+RPL    
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLDFPKRPLTGPA 422

Query: 426 RADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAI 485
           +   SIIN TY+GF+EV+ QNNDT + S HM+GY+FFV GM YG+W+E+ RG+YNKWD I
Sbjct: 423 KVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGI 482

Query: 486 ARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPS 545
           AR T QVYPGAW+A+LISLDN G+WNLR ENLD WYLGQETY+R+VNP+EN KTE   P 
Sbjct: 483 ARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPD 542

Query: 546 NVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFI 588
           NVLYCGAL  LQK QK +++ S   G    F SL M  + +V +
Sbjct: 543 NVLYCGALSKLQKPQKVSSSASKSIG----FTSLSMVVMALVMM 582

BLAST of MS000318 vs. TAIR 10
Match: AT5G48450.1 (SKU5 similar 3 )

HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-209
Identity = 342/575 (59.48%), Postives = 433/575 (75.30%), Query Frame = 0

Query: 21  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 80
           AADPY+F+++ VSY++ASPLG  QQVI +NGQFPGP +N TTN+NV +NV N+LDE LLL
Sbjct: 24  AADPYVFFDWTVSYLSASPLGTRQQVIGINGQFPGPILNVTTNWNVVMNVKNNLDEPLLL 83

Query: 81  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 140
           TW+GIQ R+NSWQDGVLGTNCPIP  WNWTY+FQVKDQIGSF+YFPS NFQ+A+GG+G  
Sbjct: 84  TWNGIQHRKNSWQDGVLGTNCPIPSGWNWTYEFQVKDQIGSFFYFPSTNFQRASGGYGGI 143

Query: 141 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 200
           ++NNR IIP+PFA PDGD+ + I DWY ++H  LR D+++   L  PDG+++NG GP+  
Sbjct: 144 IVNNRAIIPVPFALPDGDVTLFISDWYTKSHKKLRKDVESKNGLRPPDGIVINGFGPFAS 203

Query: 201 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 260
           N      G  + TI V PG+TYR RVHN GI+TSLNFRIQ HN+LLVETEG YT+ QNYT
Sbjct: 204 N------GSPFGTINVEPGRTYRFRVHNSGIATSLNFRIQNHNLLLVETEGSYTIQQNYT 263

Query: 261 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 320
           + DIHVGQS+SFLVTMDQ+ S DYYIVAS RF  +    K +GVA+L YSNS+GPA+GPL
Sbjct: 264 NMDIHVGQSFSFLVTMDQSGSNDYYIVASPRFATSI---KASGVAVLRYSNSQGPASGPL 323

Query: 321 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKSEPLVTINR 380
           PDPP +  D   SMNQARS+R N+S+  ARPNPQGSF YGQI VT  Y++ + P   I  
Sbjct: 324 PDPPIEL-DTFFSMNQARSLRLNLSSGAARPNPQGSFKYGQITVTDVYVIVNRPPEMIEG 383

Query: 381 TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADISIINATYKGFIEV 440
             RAT NGIS++PP TP++LA Q+ + G YKLDFP+RP+NR PR D S+IN T+KGF+E+
Sbjct: 384 RLRATLNGISYLPPATPLKLAQQYNISGVYKLDFPKRPMNRHPRVDTSVINGTFKGFVEI 443

Query: 441 IFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVLI 500
           IFQN+DT + S H++GY+FFV GM +G W+E+ R +YNK DA+AR TTQV+PGAWTAVL+
Sbjct: 444 IFQNSDTTVKSYHLDGYAFFVVGMDFGLWTENSRSTYNKGDAVARSTTQVFPGAWTAVLV 503

Query: 501 SLDNVGSWNLRAENLDRWYLGQETYLRIVNPE-ENGKTEMAPPSNVLYCGALQNLQKEQK 560
           SLDN G WNLR +NL  WYLGQE YL +VNPE +   +E + P N +YCG L  LQK+Q 
Sbjct: 504 SLDNAGMWNLRIDNLASWYLGQELYLSVVNPEIDIDSSENSVPKNSIYCGRLSPLQKDQA 563

Query: 561 H-----NNADSIFKGHSKLFISLLMAFLHIVFICS 590
                  +  SIF     + ++L    ++I  +C+
Sbjct: 564 QRVNFSGSQRSIFVTSRGILLALFAILVNINRLCN 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140913.10.0e+0099.83monocopper oxidase-like protein SKS1 [Momordica charantia][more]
XP_038880777.10.0e+0091.17monocopper oxidase-like protein SKS1 [Benincasa hispida][more]
XP_008439091.10.0e+0090.69PREDICTED: monocopper oxidase-like protein SKS1 [Cucumis melo][more]
XP_011651117.10.0e+0089.86monocopper oxidase-like protein SKS1 [Cucumis sativus] >KGN57269.1 hypothetical ... [more]
KAG6576799.10.0e+0088.96Monocopper oxidase-like protein SKS1, partial [Cucurbita argyrosperma subsp. sor... [more]
Match NameE-valueIdentityDescription
Q8VXX58.9e-25973.01Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN63.2e-24870.57Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q9SU404.0e-24369.52Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q006241.3e-14044.73L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291624.7e-13544.38L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CJ410.0e+0099.83monocopper oxidase-like protein SKS1 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A1S3AXI50.0e+0090.69monocopper oxidase-like protein SKS1 OS=Cucumis melo OX=3656 GN=LOC103483983 PE=... [more]
A0A0A0L8C40.0e+0089.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G175700 PE=3 SV=1[more]
A0A6J1E5V70.0e+0088.96monocopper oxidase-like protein SKS1 OS=Cucurbita moschata OX=3662 GN=LOC1114308... [more]
A0A6J1J8990.0e+0088.62monocopper oxidase-like protein SKS1 OS=Cucurbita maxima OX=3661 GN=LOC111483473... [more]
Match NameE-valueIdentityDescription
AT4G25240.16.3e-26073.01SKU5 similar 1 [more]
AT5G51480.12.2e-24970.57SKU5 similar 2 [more]
AT4G12420.12.8e-24469.52Cupredoxin superfamily protein [more]
AT4G12420.22.8e-24469.52Cupredoxin superfamily protein [more]
AT5G48450.12.3e-20959.48SKU5 similar 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 405..532
e-value: 1.4E-25
score: 89.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 361..562
e-value: 3.4E-33
score: 117.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 20..144
e-value: 3.3E-30
score: 106.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 155..348
e-value: 5.1E-42
score: 146.2
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 21..172
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 152..351
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 377..555
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 32..146
e-value: 4.7E-40
score: 136.3
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..311
e-value: 5.6E-44
score: 150.1
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 10..560
NoneNo IPR availablePANTHERPTHR11709:SF270MONOCOPPER OXIDASE-LIKE PROTEIN SKS1coord: 10..560
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 26..143
e-value: 1.26967E-77
score: 239.231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000318.1MS000318.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity