MS000294 (gene) Bitter gourd (TR) v1

Overview
NameMS000294
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box protein
Locationscaffold44: 691134 .. 693348 (+)
RNA-Seq ExpressionMS000294
SyntenyMS000294
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTACGCGCATCAGGGACCTCAAGCGGTGCTGTTTGAAGCCGAGAGACGAAGCTCCATTTCTGGCTCTGTTCCCAGTCCGCAAACGAACCCTAGCTCGCCGGTGAAGAAGAGGGCGCCCCGCTGGTCTGATGTCTGGCTCAAGAACACCAAGTCGCTTGAAAATGTTATAGTCGCGATGCAACTTCAATCCTTGTGTAAGGACTCTCGTTCTACTCCCAGCTCCAAAACCAAACCCCTCATCACTAATTTCTGCAAACTCGACCGTACGATGCTTCTCTCCGACGAGCTTCTTCTCAAGATCCTCTCCAAGCTCCCGGATTCTCAGCGGAACTCCAACTTCCTGGTCTGCAAGCGGTGGCTGAACCTGCAGGGCCGCCTTGTACGGTCTCTCAGAGTCATGGATTTGAATTTTCTCTTGTCGGGTAGGTTGATTTTGAGGTTTCCGAATCTTAACCGAGTGGATTTAGTCTCTGGGTCTTTGATGTCTTCCAGAAATTCTAGTATACTGTTGAGTAATAGGGTTCTTTCGATGCATATTGATTCTTGGTTTTTGCCTATTCCGGGTGTGGGTGAGGAAAATATCCTAGACAATATGGTAATTGATAGAGGGCTGAAGGCGCTGGCCAGTGGCTGCCCAAATTTGCGGAAATTAGCTTTTATTGGTGGGAGCGAGTTGGGATTGTTGAGTGTGGCTGAGGAATGTGCAACTTTACAAGAACTTGAACTGCATAGGTGTAATGATAATCTTCTGCGAGGAATTGCAGCTTGTGAGAATTTACAAATACTGAAGCTAGTTGGAAATGTAGATGGTCTTTATAGCTCAGTGGTTACTGATATTGGGTTAACTATTTTGGCTCAAGGTTGTAAACGACTGGTGAAGCTTGAGCTTAATGGTTGTGAGGGAAGTTTTGATGGCATCAAGGCTATTGGGCAGTGCTGCCAAATGTTGGAGGAACTGACACTCTGTGATCATAGGATGGACAATGGATGGTTGGCTGGGCTTTCATATTGTGAGAATTTGAAGACTCTGAGGATCATGTCGTGTAGGAGGATTGATCCAAATCCTGGACCTGACGAATATTTGAGTCCCTGTCCTGCTCTCGAGCGTTTACATTTGCAGAATTGTCAACTACGAGATAAAAGGAGTGCTAAAGCATTGTTTGTTACTTGTGGGGCTGCAAGAGAGATTTTCATCCGCGATTGCTGGGGAGTGGTGGATGACATTTTCAGCTTTGCAAGTCATTGCTGGTAAATAGTCTCTCCTTTTATTTGATAATAACTTAACTTTACCAAATATTCTGTGGCTTTTAATGAAGTTCACTGTTCAAATTTCTAACTCCCTGTAGATTATTTTAGTGCAGTACACAGGGGGGGCATCTCCTTGAGTGCTCTCATAAAAGTTCTATAATCTATAACTGATATGCTTTGATGCAGGCCGTAGAAGTTGTTACAGTTTTTCTGCATAGACGTTTGGTCTGTGACTGGGATGTGTCTTAAGCCTTTTAGTACTAGAATCATGTTGTTTTTCTGGGTTAGGTTCCTGAATTTAACGCAGAACCTCTAACAGCCATGGTGAGGTTCTAGGCTGATCATTGTTCTTAGGGATCCATTGCTTCAAATAAATTGGTGAAAATGCTGGTATTTAAGTGAGGTTTGAATTACATAGAGAATCTATATCATTTATGGCGGGAATCTGGCTTTGACTGCGCCCAGTCAGTCGAGTTGCTTATGACTCAATACGGCATATCTACATTCCCCTTAAAACTTGAAAAATTAAGAATTGTTCATCCTGTTTCAATTAACCGTTTCCTTATGACTCTGGAAGAATTATTGGATTAGCACTTGTTTAGCAAGAAATTCTGTGTTTAACTTGTGGTCGCTTTTCTCACAGGAGGGTGAGGTTCCTTTCTCTAGAAGGATGTTCACTGCTAACAACAGAAGGTTTGGAGTCTGTAATTCTTCAATGGAAGGAGCTTCAAAGTCTTCGAGTCGTATCGTGTAAGAACATAAAAGAGAGCAGGATTTCTCCTGCTCTCTCTTCCCTGTTCTCCATTCTCAAGGATTTGAAATGGAGGCCAGATACCAAATCTCTTCTCCCGTCAAGTCTCATCGGCACCCACATGGGAAAGAAGGGTGGCAGGTTTTTCAAAAAAACGTGGGACATAAAAGTTCTGCCCGGTGTTCATGATCATGCAATTACACGACAAACA

mRNA sequence

ATGGGTTACGCGCATCAGGGACCTCAAGCGGTGCTGTTTGAAGCCGAGAGACGAAGCTCCATTTCTGGCTCTGTTCCCAGTCCGCAAACGAACCCTAGCTCGCCGGTGAAGAAGAGGGCGCCCCGCTGGTCTGATGTCTGGCTCAAGAACACCAAGTCGCTTGAAAATGTTATAGTCGCGATGCAACTTCAATCCTTGTGTAAGGACTCTCGTTCTACTCCCAGCTCCAAAACCAAACCCCTCATCACTAATTTCTGCAAACTCGACCGTACGATGCTTCTCTCCGACGAGCTTCTTCTCAAGATCCTCTCCAAGCTCCCGGATTCTCAGCGGAACTCCAACTTCCTGGTCTGCAAGCGGTGGCTGAACCTGCAGGGCCGCCTTGTACGGTCTCTCAGAGTCATGGATTTGAATTTTCTCTTGTCGGGTAGGTTGATTTTGAGGTTTCCGAATCTTAACCGAGTGGATTTAGTCTCTGGGTCTTTGATGTCTTCCAGAAATTCTAGTATACTGTTGAGTAATAGGGTTCTTTCGATGCATATTGATTCTTGGTTTTTGCCTATTCCGGGTGTGGGTGAGGAAAATATCCTAGACAATATGGTAATTGATAGAGGGCTGAAGGCGCTGGCCAGTGGCTGCCCAAATTTGCGGAAATTAGCTTTTATTGGTGGGAGCGAGTTGGGATTGTTGAGTGTGGCTGAGGAATGTGCAACTTTACAAGAACTTGAACTGCATAGGTGTAATGATAATCTTCTGCGAGGAATTGCAGCTTGTGAGAATTTACAAATACTGAAGCTAGTTGGAAATGTAGATGGTCTTTATAGCTCAGTGGTTACTGATATTGGGTTAACTATTTTGGCTCAAGGTTGTAAACGACTGGTGAAGCTTGAGCTTAATGGTTGTGAGGGAAGTTTTGATGGCATCAAGGCTATTGGGCAGTGCTGCCAAATGTTGGAGGAACTGACACTCTGTGATCATAGGATGGACAATGGATGGTTGGCTGGGCTTTCATATTGTGAGAATTTGAAGACTCTGAGGATCATGTCGTGTAGGAGGATTGATCCAAATCCTGGACCTGACGAATATTTGAGTCCCTGTCCTGCTCTCGAGCGTTTACATTTGCAGAATTGTCAACTACGAGATAAAAGGAGTGCTAAAGCATTGTTTGTTACTTGTGGGGCTGCAAGAGAGATTTTCATCCGCGATTGCTGGGGAGTGGTGGATGACATTTTCAGCTTTGCAAGTCATTGCTGGAGGGTGAGGTTCCTTTCTCTAGAAGGATGTTCACTGCTAACAACAGAAGGTTTGGAGTCTGTAATTCTTCAATGGAAGGAGCTTCAAAGTCTTCGAGTCGTATCGTGTAAGAACATAAAAGAGAGCAGGATTTCTCCTGCTCTCTCTTCCCTGTTCTCCATTCTCAAGGATTTGAAATGGAGGCCAGATACCAAATCTCTTCTCCCGTCAAGTCTCATCGGCACCCACATGGGAAAGAAGGGTGGCAGGTTTTTCAAAAAAACGTGGGACATAAAAGTTCTGCCCGGTGTTCATGATCATGCAATTACACGACAAACA

Coding sequence (CDS)

ATGGGTTACGCGCATCAGGGACCTCAAGCGGTGCTGTTTGAAGCCGAGAGACGAAGCTCCATTTCTGGCTCTGTTCCCAGTCCGCAAACGAACCCTAGCTCGCCGGTGAAGAAGAGGGCGCCCCGCTGGTCTGATGTCTGGCTCAAGAACACCAAGTCGCTTGAAAATGTTATAGTCGCGATGCAACTTCAATCCTTGTGTAAGGACTCTCGTTCTACTCCCAGCTCCAAAACCAAACCCCTCATCACTAATTTCTGCAAACTCGACCGTACGATGCTTCTCTCCGACGAGCTTCTTCTCAAGATCCTCTCCAAGCTCCCGGATTCTCAGCGGAACTCCAACTTCCTGGTCTGCAAGCGGTGGCTGAACCTGCAGGGCCGCCTTGTACGGTCTCTCAGAGTCATGGATTTGAATTTTCTCTTGTCGGGTAGGTTGATTTTGAGGTTTCCGAATCTTAACCGAGTGGATTTAGTCTCTGGGTCTTTGATGTCTTCCAGAAATTCTAGTATACTGTTGAGTAATAGGGTTCTTTCGATGCATATTGATTCTTGGTTTTTGCCTATTCCGGGTGTGGGTGAGGAAAATATCCTAGACAATATGGTAATTGATAGAGGGCTGAAGGCGCTGGCCAGTGGCTGCCCAAATTTGCGGAAATTAGCTTTTATTGGTGGGAGCGAGTTGGGATTGTTGAGTGTGGCTGAGGAATGTGCAACTTTACAAGAACTTGAACTGCATAGGTGTAATGATAATCTTCTGCGAGGAATTGCAGCTTGTGAGAATTTACAAATACTGAAGCTAGTTGGAAATGTAGATGGTCTTTATAGCTCAGTGGTTACTGATATTGGGTTAACTATTTTGGCTCAAGGTTGTAAACGACTGGTGAAGCTTGAGCTTAATGGTTGTGAGGGAAGTTTTGATGGCATCAAGGCTATTGGGCAGTGCTGCCAAATGTTGGAGGAACTGACACTCTGTGATCATAGGATGGACAATGGATGGTTGGCTGGGCTTTCATATTGTGAGAATTTGAAGACTCTGAGGATCATGTCGTGTAGGAGGATTGATCCAAATCCTGGACCTGACGAATATTTGAGTCCCTGTCCTGCTCTCGAGCGTTTACATTTGCAGAATTGTCAACTACGAGATAAAAGGAGTGCTAAAGCATTGTTTGTTACTTGTGGGGCTGCAAGAGAGATTTTCATCCGCGATTGCTGGGGAGTGGTGGATGACATTTTCAGCTTTGCAAGTCATTGCTGGAGGGTGAGGTTCCTTTCTCTAGAAGGATGTTCACTGCTAACAACAGAAGGTTTGGAGTCTGTAATTCTTCAATGGAAGGAGCTTCAAAGTCTTCGAGTCGTATCGTGTAAGAACATAAAAGAGAGCAGGATTTCTCCTGCTCTCTCTTCCCTGTTCTCCATTCTCAAGGATTTGAAATGGAGGCCAGATACCAAATCTCTTCTCCCGTCAAGTCTCATCGGCACCCACATGGGAAAGAAGGGTGGCAGGTTTTTCAAAAAAACGTGGGACATAAAAGTTCTGCCCGGTGTTCATGATCATGCAATTACACGACAAACA

Protein sequence

MGYAHQGPQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVAMQLQSLCKDSRSTPSSKTKPLITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVRFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPDTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT
Homology
BLAST of MS000294 vs. NCBI nr
Match: XP_022141321.1 (F-box protein At5g51380-like [Momordica charantia])

HSP 1 Score: 1044.6 bits (2700), Expect = 2.8e-301
Identity = 518/524 (98.85%), Postives = 518/524 (98.85%), Query Frame = 0

Query: 1   MGYAHQGPQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVA 60
           MGYAH G QAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVA
Sbjct: 1   MGYAHHGAQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVA 60

Query: 61  MQLQSLCKDSRSTPSSKTKPLITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKR 120
           MQLQSLCKDSRSTPSSKTK LIT FCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKR
Sbjct: 61  MQLQSLCKDSRSTPSSKTKSLITKFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKR 120

Query: 121 WLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMH 180
           WLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMH
Sbjct: 121 WLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMH 180

Query: 181 IDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQ 240
           IDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQ
Sbjct: 181 IDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQ 240

Query: 241 ELELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNG 300
           ELELHRCNDN LRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNG
Sbjct: 241 ELELHRCNDNFLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNG 300

Query: 301 CEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPD 360
           CEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPD
Sbjct: 301 CEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPD 360

Query: 361 EYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRV 420
           EYLSPCPALE LHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRV
Sbjct: 361 EYLSPCPALEHLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRV 420

Query: 421 RFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRP 480
           RFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRP
Sbjct: 421 RFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRP 480

Query: 481 DTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           DTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT
Sbjct: 481 DTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 524

BLAST of MS000294 vs. NCBI nr
Match: XP_038879973.1 (F-box protein At5g51380-like [Benincasa hispida])

HSP 1 Score: 965.3 bits (2494), Expect = 2.1e-277
Identity = 476/526 (90.49%), Postives = 497/526 (94.49%), Query Frame = 0

Query: 1   MGYAHQGPQAVLFEAERRSSISGSVPSPQTNPSSPV-KKRAPRWSDVWLKNTKSLENVIV 60
           MGYA+Q PQ+VLFEAERR S   SVPSPQTNPSSPV KKR PRWSDVWLKNTKSLE+VIV
Sbjct: 1   MGYANQRPQSVLFEAERRRSSISSVPSPQTNPSSPVKKKRTPRWSDVWLKNTKSLEHVIV 60

Query: 61  AMQLQSLCKDSR-STPSSKTKPLITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVC 120
           AMQLQSL KDS   TP+SKTKPL+ NFCK+DRT+LLSDELLL+ILSKLPDSQRNS FLVC
Sbjct: 61  AMQLQSLSKDSHIPTPNSKTKPLLANFCKIDRTLLLSDELLLRILSKLPDSQRNSTFLVC 120

Query: 121 KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLS 180
           KRWLNLQGRLVRSL VMDLNFLLSGRLI RFPNLNRVDLVSGSLMSSRNS ILLSNR+ S
Sbjct: 121 KRWLNLQGRLVRSLGVMDLNFLLSGRLISRFPNLNRVDLVSGSLMSSRNSGILLSNRIFS 180

Query: 181 MHIDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECAT 240
           MH+DSWFLPIPGVGEE ILDNMVIDRGLK LASGCPNLRKLAFIGGSELGLLSVAEEC T
Sbjct: 181 MHVDSWFLPIPGVGEEIILDNMVIDRGLKTLASGCPNLRKLAFIGGSELGLLSVAEECVT 240

Query: 241 LQELELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300
           LQELELH+CNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL
Sbjct: 241 LQELELHKCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300

Query: 301 NGCEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPG 360
           NGCEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLS+CENLKTLRIMSCR+IDPNPG
Sbjct: 301 NGCEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSFCENLKTLRIMSCRKIDPNPG 360

Query: 361 PDEYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCW 420
           PDEYLSPCPALERLHLQNCQLRD++SA+ALF+TCGAAREIFI DCWG+VD IFSFASHCW
Sbjct: 361 PDEYLSPCPALERLHLQNCQLRDRKSARALFITCGAAREIFISDCWGLVDGIFSFASHCW 420

Query: 421 RVRFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKW 480
           RV+FLSLEGCSLLTTEGLESVILQW ELQSLRVVSCKNIKES ISPALSSLFS  KDLKW
Sbjct: 421 RVKFLSLEGCSLLTTEGLESVILQWNELQSLRVVSCKNIKESSISPALSSLFSTFKDLKW 480

Query: 481 RPDTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           RPD+KSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGV+DH   RQT
Sbjct: 481 RPDSKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVYDHTSARQT 526

BLAST of MS000294 vs. NCBI nr
Match: XP_008453917.1 (PREDICTED: F-box protein At5g51380-like [Cucumis melo])

HSP 1 Score: 955.3 bits (2468), Expect = 2.2e-274
Identity = 469/526 (89.16%), Postives = 494/526 (93.92%), Query Frame = 0

Query: 1   MGYAHQGPQAVLFEAERRSSISGSVPSPQTNPSSPV-KKRAPRWSDVWLKNTKSLENVIV 60
           MG+A+   Q+VL E ERR S   SVPSPQTNP+SPV KKR PRWSDVWLKNT +LENVIV
Sbjct: 1   MGFANHRQQSVLLEGERRRSSISSVPSPQTNPNSPVKKKRTPRWSDVWLKNTNTLENVIV 60

Query: 61  AMQLQSLCKDSR-STPSSKTKPLITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVC 120
           AMQLQS  KDS   TP+SKTKPL+ NFCK+DRT+LLSDELLL+ILSKLPDSQRNSNFLVC
Sbjct: 61  AMQLQSFSKDSHIPTPNSKTKPLLANFCKIDRTLLLSDELLLRILSKLPDSQRNSNFLVC 120

Query: 121 KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLS 180
           KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNS ILLSNR+LS
Sbjct: 121 KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSGILLSNRILS 180

Query: 181 MHIDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECAT 240
           MH+DSWFLP+PGVGEENILDNMVID+GLK LASGCPNLRKLAFIGGSELGLLSVAEEC T
Sbjct: 181 MHVDSWFLPVPGVGEENILDNMVIDKGLKTLASGCPNLRKLAFIGGSELGLLSVAEECET 240

Query: 241 LQELELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300
           LQELELH+CNDNLLRGIAACENLQILKL+GNVDGLYSSVVTDIGLTILAQGCKRLVKLEL
Sbjct: 241 LQELELHKCNDNLLRGIAACENLQILKLIGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300

Query: 301 NGCEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPG 360
           NGCEGSFDGIKAIGQCCQMLEELT+CDHRMD+GWLA LSYCENLKTLRIMSCR+IDPNPG
Sbjct: 301 NGCEGSFDGIKAIGQCCQMLEELTICDHRMDDGWLAALSYCENLKTLRIMSCRKIDPNPG 360

Query: 361 PDEYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCW 420
           PDEYLSPCPALERLHL NCQLRD++SAKALFVTCGAAREIFI DCWG+VD IFSFASH W
Sbjct: 361 PDEYLSPCPALERLHLHNCQLRDRKSAKALFVTCGAAREIFISDCWGLVDGIFSFASHFW 420

Query: 421 RVRFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKW 480
           RV+FLSLEGCSLLTT+GLESVILQW ELQSLRVVSCKNIKES ISPALSSLFSI KDLKW
Sbjct: 421 RVKFLSLEGCSLLTTQGLESVILQWNELQSLRVVSCKNIKESSISPALSSLFSIFKDLKW 480

Query: 481 RPDTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           RPDTKSLL SSLIGTHMGKKGGRFFKKTWD+KVLPGVHDH  TRQT
Sbjct: 481 RPDTKSLLSSSLIGTHMGKKGGRFFKKTWDMKVLPGVHDHTSTRQT 526

BLAST of MS000294 vs. NCBI nr
Match: XP_023528939.1 (F-box protein At5g51370-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 949.5 bits (2453), Expect = 1.2e-272
Identity = 469/523 (89.67%), Postives = 495/523 (94.65%), Query Frame = 0

Query: 4   AHQGPQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVAMQL 63
           AH   Q+VLFEAERRSSIS SVPSPQTNP+ P KKR PRWSDVWLK+TKSLENVIVAMQL
Sbjct: 3   AHGRAQSVLFEAERRSSIS-SVPSPQTNPNLPGKKRTPRWSDVWLKDTKSLENVIVAMQL 62

Query: 64  QSLCKDSR-STPSSKTKP-LITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRW 123
           QS CKDS+  TP+SK  P +I NFCK+DRT+LLSDELLLKIL+KLPDSQRNSNFLVCKRW
Sbjct: 63  QSSCKDSQIPTPNSKINPVVIANFCKIDRTLLLSDELLLKILTKLPDSQRNSNFLVCKRW 122

Query: 124 LNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHI 183
           LNLQGR+VRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLM SRNS ILLSNR+ SMH+
Sbjct: 123 LNLQGRIVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMCSRNSGILLSNRIHSMHV 182

Query: 184 DSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQE 243
           DSWFLPIPGVGEENILDNMVIDRGLKALA GCPNLRKLAFIGGSELGLL+VAEEC TLQE
Sbjct: 183 DSWFLPIPGVGEENILDNMVIDRGLKALADGCPNLRKLAFIGGSELGLLNVAEECETLQE 242

Query: 244 LELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGC 303
           LELH+CNDNLLRGIAACENLQILKLVGNVDGLYSSVVTD+GLTILAQGCKRLVKLELNGC
Sbjct: 243 LELHKCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDVGLTILAQGCKRLVKLELNGC 302

Query: 304 EGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDE 363
           EGSFDGIKAIGQCCQMLEELT+CDHRMDNGWLAGLSY ENLKTLRI+SCRRIDPNPGPDE
Sbjct: 303 EGSFDGIKAIGQCCQMLEELTICDHRMDNGWLAGLSYSENLKTLRIVSCRRIDPNPGPDE 362

Query: 364 YLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVR 423
           YLSPCPALERLHLQNCQLRDK+SAKALF+TC AAREIFI DCWG+ DD+FSFASHCWRV+
Sbjct: 363 YLSPCPALERLHLQNCQLRDKKSAKALFITCSAAREIFIGDCWGLADDLFSFASHCWRVK 422

Query: 424 FLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPD 483
           FLSL+GCSLLTTEGLESVILQWKELQSLRVVSCKNIKES ISPALSSLFSI KDLKWRPD
Sbjct: 423 FLSLKGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESSISPALSSLFSIFKDLKWRPD 482

Query: 484 TKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           TKSLLPS+LI THMGKKGGRFFKKTWDIKVLPGVH H+ TR+T
Sbjct: 483 TKSLLPSTLIHTHMGKKGGRFFKKTWDIKVLPGVHGHSSTRKT 524

BLAST of MS000294 vs. NCBI nr
Match: XP_022942171.1 (F-box protein At5g51370-like [Cucurbita moschata])

HSP 1 Score: 946.8 bits (2446), Expect = 7.8e-272
Identity = 468/523 (89.48%), Postives = 493/523 (94.26%), Query Frame = 0

Query: 4   AHQGPQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVAMQL 63
           AH   Q+VLFEAERRSSIS SVPSPQTNP+ P KKR PRWSDVWLK+TKSLENVIVAMQL
Sbjct: 3   AHGRAQSVLFEAERRSSIS-SVPSPQTNPNLPGKKRTPRWSDVWLKDTKSLENVIVAMQL 62

Query: 64  QSLCKDSR-STPSSKTKP-LITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRW 123
           QS CKDS+  TP+SK  P LI NFCK+DRT+LLSDELLL+IL+KLPDSQRNSNFLVCKRW
Sbjct: 63  QSSCKDSQIPTPNSKINPILIANFCKIDRTLLLSDELLLRILTKLPDSQRNSNFLVCKRW 122

Query: 124 LNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHI 183
           LNLQGR+VRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLM SRNS ILLSNR+ SMH+
Sbjct: 123 LNLQGRIVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMCSRNSGILLSNRIHSMHV 182

Query: 184 DSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQE 243
           DSWFLPIPGVGEENILDNMVIDRGLKALA GCPNLRKLAFIGGSELGLL+VAEEC TLQE
Sbjct: 183 DSWFLPIPGVGEENILDNMVIDRGLKALADGCPNLRKLAFIGGSELGLLNVAEECETLQE 242

Query: 244 LELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGC 303
           LELH+CNDNLLRGIAACENLQILKLVGNVDGLYSSVVTD+GLTILAQGCKRLVKLELNGC
Sbjct: 243 LELHKCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDVGLTILAQGCKRLVKLELNGC 302

Query: 304 EGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDE 363
           EGSFDGIKAIGQCCQMLEELT+CDHRMDNGWLAGLSY ENLKTLRI+SCRRIDPNPGPDE
Sbjct: 303 EGSFDGIKAIGQCCQMLEELTICDHRMDNGWLAGLSYSENLKTLRIVSCRRIDPNPGPDE 362

Query: 364 YLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVR 423
           YLSPCPALERLHLQNCQLRDK+SAKALF+TC AAREIFI DCWG+ DD+FSFASHCWRV+
Sbjct: 363 YLSPCPALERLHLQNCQLRDKKSAKALFITCSAAREIFISDCWGLADDLFSFASHCWRVK 422

Query: 424 FLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPD 483
           FLSL+GCSLLTTEGLESVILQWKELQSLRVVSCKNIKES ISPALSSLFSI KDLKWRP 
Sbjct: 423 FLSLKGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESSISPALSSLFSIFKDLKWRPH 482

Query: 484 TKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           TKSLLPS+LI THMGKKGGRFFKKTWDIKVLPGVH H  TR+T
Sbjct: 483 TKSLLPSTLIHTHMGKKGGRFFKKTWDIKVLPGVHGHTSTRKT 524

BLAST of MS000294 vs. ExPASy Swiss-Prot
Match: Q9FGN3 (F-box protein At5g51380 OS=Arabidopsis thaliana OX=3702 GN=At5g51380 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.7e-158
Identity = 282/476 (59.24%), Postives = 355/476 (74.58%), Query Frame = 0

Query: 30  TNPSSPVKKRAPRWSDVWLKNTKSLENVIVAMQLQSLCKDSRSTPSSKTKPLITNFCKLD 89
           T+P SP+++R   W+  WL +  +     V+  +Q+       +P S+ K L ++F  +D
Sbjct: 9   TSPKSPLRRRRSSWTGTWLNHQTTSFKQAVSAVIQA------QSPRSRFKSLSSDFSDVD 68

Query: 90  RTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLILRF 149
           RT+ LSD LLLKIL KLP+SQ     LVCKRWL++QGR +RS++V D  FLLSGRL+ RF
Sbjct: 69  RTLSLSDSLLLKILEKLPESQNEDVSLVCKRWLSVQGRRLRSMKVFDWEFLLSGRLVSRF 128

Query: 150 PNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDSWFLPIPGVGEENILDNMVIDRGLKAL 209
           P L  VDLV+     S NS ILL +  +S H+ +         EE++LDN ++D+GL+ L
Sbjct: 129 PKLTSVDLVNACFNPSSNSGILLCHTSISFHVSTDSSLNLNFVEESLLDNEMVDKGLRVL 188

Query: 210 ASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENLQILKLVGN 269
             G  +L KL  I  +ELGLLS+AE+C+ LQELELH+C+DNLLRGIAACENL+ L+LVG+
Sbjct: 189 GRGSFDLIKLVVINATELGLLSLAEDCSDLQELELHKCSDNLLRGIAACENLRGLRLVGS 248

Query: 270 VDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEELTLCDHRMD 329
           VDGLYSS V+DIGLTILAQGCKRLVKLEL+GCEGSFDGIKAIGQCC++LEEL++CDHRMD
Sbjct: 249 VDGLYSSSVSDIGLTILAQGCKRLVKLELSGCEGSFDGIKAIGQCCEVLEELSICDHRMD 308

Query: 330 NGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQNCQLRDKRSAKALF 389
           +GW+A LSY E+LKTL I SCR+ID +PGP + L  CPALE L L+ C L DK   +ALF
Sbjct: 309 DGWIAALSYFESLKTLLISSCRKIDSSPGPGKLLGSCPALESLQLRRCCLNDKEGMRALF 368

Query: 390 VTCGAAREIFIRDCWGVVDDIFSFASHCWRVRFLSLEGCSLLTTEGLESVILQWKELQSL 449
             C    ++ I+DCWG+ DD FS A    RVRFLSLEGCS+LTT GLESVIL W+EL+S+
Sbjct: 369 KVCDGVTKVNIQDCWGLDDDSFSLAKAFRRVRFLSLEGCSILTTSGLESVILHWEELESM 428

Query: 450 RVVSCKNIKESRISPALSSLFSILKDLKWRPDTKSLLPSSLIGTHMGKKGGRFFKK 506
           RVVSCKNIK+S IS ALSSLFS+LK+L WRPDT+S L SSL GT +GK+G +FFKK
Sbjct: 429 RVVSCKNIKDSEISAALSSLFSLLKELTWRPDTRSHLSSSLEGTGIGKRGSKFFKK 478

BLAST of MS000294 vs. ExPASy Swiss-Prot
Match: Q8VYT5 (F-box protein At5g07670 OS=Arabidopsis thaliana OX=3702 GN=At5g07670 PE=2 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.9e-157
Identity = 287/481 (59.67%), Postives = 364/481 (75.68%), Query Frame = 0

Query: 29  QTNPSSPVKKRAPRWSDVWLKNTKSLEN--VIVAMQLQSLCKDSRSTPSSKTKPLITNFC 88
           +++P SP+KKR   WS++W+ +   L +  + +A + QSL     + P SK+K L+ +F 
Sbjct: 8   ESSPVSPLKKRRASWSELWVNHHHLLSSSPLDLAAKFQSL-----TPPISKSKTLLPDF- 67

Query: 89  KLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLI 148
               T+LL D +L++++ K+P+SQR +  LVCKRW  L GRLVRS +V D  FL SGRLI
Sbjct: 68  ----TLLLPDLILIRVIQKIPNSQRKNLSLVCKRWFRLHGRLVRSFKVSDWEFLSSGRLI 127

Query: 149 LRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDSWFLPIPGVGEENILDNMVIDRGL 208
            RFPNL  VDLVSG L+S  N  IL+++R++S  +           EEN+L   +++RGL
Sbjct: 128 SRFPNLETVDLVSGCLISPPNLGILVNHRIVSFTVGVGSYQSWSFFEENLLSVELVERGL 187

Query: 209 KALASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENLQILKL 268
           KALA GC NLRKL     SELGLL+VAEEC+ LQELELH+C+D++L GI A ENLQIL+L
Sbjct: 188 KALAGGCSNLRKLVVTNTSELGLLNVAEECSRLQELELHKCSDSVLLGIGAFENLQILRL 247

Query: 269 VGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEELTLCDH 328
           VGNVDGLY+S+V+DIGL ILAQGCKRLVKLEL GCEG FDGIK IG+CCQMLEELT+CD+
Sbjct: 248 VGNVDGLYNSLVSDIGLMILAQGCKRLVKLELVGCEGGFDGIKEIGECCQMLEELTVCDN 307

Query: 329 RMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLS-PCPALERLHLQNCQLRDKRSA 388
           +M++GWL GL YCENLKTL+++SC++ID +  PDE LS  CPALERL L+ CQLRDK + 
Sbjct: 308 KMESGWLGGLRYCENLKTLKLVSCKKIDND--PDESLSCCCPALERLQLEKCQLRDKNTV 367

Query: 389 KALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVRFLSLEGCSLLTTEGLESVILQWKE 448
           KALF  C AAREI  +DCWG+ +DIFS A    RV+ L LEGCSLLTT GLESVIL W E
Sbjct: 368 KALFKMCEAAREIVFQDCWGLDNDIFSLAMAFGRVKLLYLEGCSLLTTSGLESVILHWHE 427

Query: 449 LQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPDTKSLLPSSLIGTHMGKKGGRFFKK 507
           L+ L+VVSCKNIK+S +SP+LS+LFS L +L+WRPDT+S L SSL GT +G+KGG+FFKK
Sbjct: 428 LEHLKVVSCKNIKDSEVSPSLSALFSALVELQWRPDTRSHLSSSLTGTGIGEKGGKFFKK 476

BLAST of MS000294 vs. ExPASy Swiss-Prot
Match: Q9FGN4 (F-box protein At5g51370 OS=Arabidopsis thaliana OX=3702 GN=At5g51370 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.4e-146
Identity = 264/425 (62.12%), Postives = 327/425 (76.94%), Query Frame = 0

Query: 87  KLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLI 146
           ++D T+ LSD LLLKI+ KLP+SQ N   LVCKRWLNLQG+ +RSL+++D +FLLS RL 
Sbjct: 25  EIDSTLSLSDSLLLKIIEKLPESQSNDVSLVCKRWLNLQGQRLRSLKLLDFDFLLSERLT 84

Query: 147 LRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDS----W-FLPIPGVGEENILDNMV 206
            RFPNL  VDLV+  +    NS IL  ++ +S H+ S    W FL      EEN+L + V
Sbjct: 85  TRFPNLTHVDLVNACMNPRVNSGILFCHKSISFHLSSDSSNWEFL------EENLLHSDV 144

Query: 207 IDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENL 266
           IDRGL+ L+    +L  L  I  SELGLLS+A +C+ LQELELH+CNDNLL GIAAC+NL
Sbjct: 145 IDRGLRILSRESFDLLNLKVINASELGLLSLAGDCSDLQELELHKCNDNLLHGIAACKNL 204

Query: 267 QILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEEL 326
           + L+LVG+VDGLYSS V+DIGLT LAQGC+ LVKLEL+GCEGSFDGIKAIGQCC++LEEL
Sbjct: 205 KGLRLVGSVDGLYSSSVSDIGLTFLAQGCRSLVKLELSGCEGSFDGIKAIGQCCEVLEEL 264

Query: 327 TLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQNCQLRD 386
           ++CDHRMD+GW+A LSY E+LK LRI SCR+ID +PGP++ L  CPA+E L L+ C L D
Sbjct: 265 SICDHRMDDGWIAALSYFESLKILRISSCRKIDASPGPEKLLRSCPAMESLQLKRCCLND 324

Query: 387 KRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVRFLSLEGCSLLTTEGLESVIL 446
           K   KALF  C  A E+ I+DCWG+ DD FS A    RVRFLSLEGCS+LT+ GLESVIL
Sbjct: 325 KEGIKALFKVCDGATEVNIQDCWGLSDDCFSLAKAFRRVRFLSLEGCSVLTSGGLESVIL 384

Query: 447 QWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPDTKSLLPSSLIGTHMGKKGGR 506
            W+EL+S+RVVSCK+IK+S ISPALSSLFS+LK+L WRPDT+S L SSL G  +G +G +
Sbjct: 385 HWEELESMRVVSCKSIKDSEISPALSSLFSLLKELTWRPDTRSHLSSSLEGAGIGIRGSK 443

BLAST of MS000294 vs. ExPASy Swiss-Prot
Match: Q9C5D2 (F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.2e-22
Identity = 104/388 (26.80%), Postives = 176/388 (45.36%), Query Frame = 0

Query: 94  LSDELLLKILSKLPDS-QRNSNFLVCKRWLNLQGRLVRSLRV-----MDLNFLLSGRLIL 153
           L +EL+L+I  +L     R++  LVCKRWL+L+     +LR+      D    L  R  L
Sbjct: 11  LPEELILEIFRRLESKPNRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRFL 70

Query: 154 RFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDSWFLPIPG---VGEENILDNMVIDR 213
              +++  + +S SL S   S      R  S    S    +      G EN+  + + D 
Sbjct: 71  YITSIHVDERISVSLPSLSPSPKRKRGRDSSSPSSSKRKKLTDKTHSGAENVESSSLTDT 130

Query: 214 GLKALASGCPNLRKLAFI---GGSELGLLSVAEECATLQELELHRC--NDNLLRGIAA-C 273
           GL ALA+G P +  L+ I     S +GL S+A++C +L+ L+L  C   D  L  +   C
Sbjct: 131 GLTALANGFPRIENLSLIWCPNVSSVGLCSLAQKCTSLKSLDLQGCYVGDQGLAAVGKFC 190

Query: 274 ENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFD--GIKAIGQCCQ 333
           + L+ L L       +   +TD+G+  L  GC + +K              ++A+G  C+
Sbjct: 191 KQLEELNL------RFCEGLTDVGVIDLVVGCSKSLKSIGVAASAKITDLSLEAVGSHCK 250

Query: 334 MLEELTL-CDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQ 393
           +LE L L  ++  D G +A    C  LK L++      D        L  C +LERL L 
Sbjct: 251 LLEVLYLDSEYIHDKGLIAVAQGCHRLKNLKLQCVSVTDVAFAAVGEL--CTSLERLALY 310

Query: 394 NCQLRDKRSAKALFVTCGAAREIFIRDCWGV-VDDIFSFASHCWRVRFLSLEGCSLLTTE 453
           + Q    +  +A+       +++ + DC+ V    + + A  C  +  + + GC  + T 
Sbjct: 311 SFQHFTDKGMRAIGKGSKKLKDLTLSDCYFVSCKGLEAIAHGCKELERVEINGCHNIGTR 370

Query: 454 GLESVILQWKELQSLRVVSCKNIKESRI 463
           G+E++      L+ L ++ C+ I  S +
Sbjct: 371 GIEAIGKSCPRLKELALLYCQRIGNSAL 390

BLAST of MS000294 vs. ExPASy Swiss-Prot
Match: Q5BJ29 (F-box/LRR-repeat protein 7 OS=Mus musculus OX=10090 GN=Fbxl7 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 8.8e-16
Identity = 105/405 (25.93%), Postives = 168/405 (41.48%), Query Frame = 0

Query: 74  PSSKTKPLI-------TNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNL-- 133
           P+  T PLI            +DR   L D  +++I S LP +Q      VC+RW NL  
Sbjct: 93  PTRLTHPLIRLASRPQKEQASIDR---LPDHSMVQIFSFLPTNQLCRCARVCRRWYNLAW 152

Query: 134 QGRLVRSLRVMDLNFLLSGRLILRFPNLNR-VDLVSGSLMSSRNSSILLSNRVLSMHIDS 193
             RL R++R       L+G  I    N++R + +++  L     +  L+   V+      
Sbjct: 153 DPRLWRTIR-------LTGETI----NVDRALKVLTRRLCQDTPNVCLMLETVI------ 212

Query: 194 WFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGG---SELGLLSVAEECATLQ 253
               + G          + DRGL  +A  CP LR+L   G    S   +  V   C  L+
Sbjct: 213 ----VSGC-------RRLTDRGLYTIAQCCPELRRLEVSGCYNISNEAVFDVVSLCPNLE 272

Query: 254 ELELHRCNDNLLRGIAACENLQILKLVGN------VDGLYSSVVTDIGLTILAQGCKRLV 313
            L++  C+      +    ++++  L G       +D     V+ D GL  +A  C +L 
Sbjct: 273 HLDVSGCSKVTCISLTREASIKLSPLHGKQISIRYLDMTDCFVLEDEGLHTIAAHCTQLT 332

Query: 314 KLELNGCEGSFD-GIKAIGQCCQMLEELTLCDHRM--DNGWLAGLSYCENLKTLRIMSCR 373
            L L  C    D G++ +   C  ++EL++ D R   D G          L+ L I  C 
Sbjct: 333 HLYLRRCVRLTDEGLRYLVIYCTSIKELSVSDCRFVSDFGLREIAKLESRLRYLSIAHCG 392

Query: 374 RIDPNPGPDEYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVD-DI 433
           RI  + G       C  L  L+ + C+       + L   C   + + I  C  V D  +
Sbjct: 393 RI-TDVGIRYVAKYCSKLRYLNARGCEGITDHGVEYLAKNCTKLKSLDIGKCPLVSDTGL 452

Query: 434 FSFASHCWRVRFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCK 456
            S A +C+ ++ LSL+ C  +T +GL+ V     +LQ L V  C+
Sbjct: 453 ESLALNCFNLKRLSLKSCESITGQGLQIVAANCFDLQMLNVQDCE 465

BLAST of MS000294 vs. ExPASy TrEMBL
Match: A0A6J1CIT9 (F-box protein At5g51380-like OS=Momordica charantia OX=3673 GN=LOC111011743 PE=4 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.3e-301
Identity = 518/524 (98.85%), Postives = 518/524 (98.85%), Query Frame = 0

Query: 1   MGYAHQGPQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVA 60
           MGYAH G QAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVA
Sbjct: 1   MGYAHHGAQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVA 60

Query: 61  MQLQSLCKDSRSTPSSKTKPLITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKR 120
           MQLQSLCKDSRSTPSSKTK LIT FCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKR
Sbjct: 61  MQLQSLCKDSRSTPSSKTKSLITKFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKR 120

Query: 121 WLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMH 180
           WLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMH
Sbjct: 121 WLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMH 180

Query: 181 IDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQ 240
           IDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQ
Sbjct: 181 IDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQ 240

Query: 241 ELELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNG 300
           ELELHRCNDN LRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNG
Sbjct: 241 ELELHRCNDNFLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNG 300

Query: 301 CEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPD 360
           CEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPD
Sbjct: 301 CEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPD 360

Query: 361 EYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRV 420
           EYLSPCPALE LHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRV
Sbjct: 361 EYLSPCPALEHLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRV 420

Query: 421 RFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRP 480
           RFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRP
Sbjct: 421 RFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRP 480

Query: 481 DTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           DTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT
Sbjct: 481 DTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 524

BLAST of MS000294 vs. ExPASy TrEMBL
Match: A0A1S3BYL5 (F-box protein At5g51380-like OS=Cucumis melo OX=3656 GN=LOC103494490 PE=4 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 469/526 (89.16%), Postives = 494/526 (93.92%), Query Frame = 0

Query: 1   MGYAHQGPQAVLFEAERRSSISGSVPSPQTNPSSPV-KKRAPRWSDVWLKNTKSLENVIV 60
           MG+A+   Q+VL E ERR S   SVPSPQTNP+SPV KKR PRWSDVWLKNT +LENVIV
Sbjct: 1   MGFANHRQQSVLLEGERRRSSISSVPSPQTNPNSPVKKKRTPRWSDVWLKNTNTLENVIV 60

Query: 61  AMQLQSLCKDSR-STPSSKTKPLITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVC 120
           AMQLQS  KDS   TP+SKTKPL+ NFCK+DRT+LLSDELLL+ILSKLPDSQRNSNFLVC
Sbjct: 61  AMQLQSFSKDSHIPTPNSKTKPLLANFCKIDRTLLLSDELLLRILSKLPDSQRNSNFLVC 120

Query: 121 KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLS 180
           KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNS ILLSNR+LS
Sbjct: 121 KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSGILLSNRILS 180

Query: 181 MHIDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECAT 240
           MH+DSWFLP+PGVGEENILDNMVID+GLK LASGCPNLRKLAFIGGSELGLLSVAEEC T
Sbjct: 181 MHVDSWFLPVPGVGEENILDNMVIDKGLKTLASGCPNLRKLAFIGGSELGLLSVAEECET 240

Query: 241 LQELELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300
           LQELELH+CNDNLLRGIAACENLQILKL+GNVDGLYSSVVTDIGLTILAQGCKRLVKLEL
Sbjct: 241 LQELELHKCNDNLLRGIAACENLQILKLIGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300

Query: 301 NGCEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPG 360
           NGCEGSFDGIKAIGQCCQMLEELT+CDHRMD+GWLA LSYCENLKTLRIMSCR+IDPNPG
Sbjct: 301 NGCEGSFDGIKAIGQCCQMLEELTICDHRMDDGWLAALSYCENLKTLRIMSCRKIDPNPG 360

Query: 361 PDEYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCW 420
           PDEYLSPCPALERLHL NCQLRD++SAKALFVTCGAAREIFI DCWG+VD IFSFASH W
Sbjct: 361 PDEYLSPCPALERLHLHNCQLRDRKSAKALFVTCGAAREIFISDCWGLVDGIFSFASHFW 420

Query: 421 RVRFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKW 480
           RV+FLSLEGCSLLTT+GLESVILQW ELQSLRVVSCKNIKES ISPALSSLFSI KDLKW
Sbjct: 421 RVKFLSLEGCSLLTTQGLESVILQWNELQSLRVVSCKNIKESSISPALSSLFSIFKDLKW 480

Query: 481 RPDTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           RPDTKSLL SSLIGTHMGKKGGRFFKKTWD+KVLPGVHDH  TRQT
Sbjct: 481 RPDTKSLLSSSLIGTHMGKKGGRFFKKTWDMKVLPGVHDHTSTRQT 526

BLAST of MS000294 vs. ExPASy TrEMBL
Match: A0A6J1FN36 (F-box protein At5g51370-like OS=Cucurbita moschata OX=3662 GN=LOC111447311 PE=4 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.8e-272
Identity = 468/523 (89.48%), Postives = 493/523 (94.26%), Query Frame = 0

Query: 4   AHQGPQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVAMQL 63
           AH   Q+VLFEAERRSSIS SVPSPQTNP+ P KKR PRWSDVWLK+TKSLENVIVAMQL
Sbjct: 3   AHGRAQSVLFEAERRSSIS-SVPSPQTNPNLPGKKRTPRWSDVWLKDTKSLENVIVAMQL 62

Query: 64  QSLCKDSR-STPSSKTKP-LITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRW 123
           QS CKDS+  TP+SK  P LI NFCK+DRT+LLSDELLL+IL+KLPDSQRNSNFLVCKRW
Sbjct: 63  QSSCKDSQIPTPNSKINPILIANFCKIDRTLLLSDELLLRILTKLPDSQRNSNFLVCKRW 122

Query: 124 LNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHI 183
           LNLQGR+VRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLM SRNS ILLSNR+ SMH+
Sbjct: 123 LNLQGRIVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMCSRNSGILLSNRIHSMHV 182

Query: 184 DSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQE 243
           DSWFLPIPGVGEENILDNMVIDRGLKALA GCPNLRKLAFIGGSELGLL+VAEEC TLQE
Sbjct: 183 DSWFLPIPGVGEENILDNMVIDRGLKALADGCPNLRKLAFIGGSELGLLNVAEECETLQE 242

Query: 244 LELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGC 303
           LELH+CNDNLLRGIAACENLQILKLVGNVDGLYSSVVTD+GLTILAQGCKRLVKLELNGC
Sbjct: 243 LELHKCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDVGLTILAQGCKRLVKLELNGC 302

Query: 304 EGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDE 363
           EGSFDGIKAIGQCCQMLEELT+CDHRMDNGWLAGLSY ENLKTLRI+SCRRIDPNPGPDE
Sbjct: 303 EGSFDGIKAIGQCCQMLEELTICDHRMDNGWLAGLSYSENLKTLRIVSCRRIDPNPGPDE 362

Query: 364 YLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVR 423
           YLSPCPALERLHLQNCQLRDK+SAKALF+TC AAREIFI DCWG+ DD+FSFASHCWRV+
Sbjct: 363 YLSPCPALERLHLQNCQLRDKKSAKALFITCSAAREIFISDCWGLADDLFSFASHCWRVK 422

Query: 424 FLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPD 483
           FLSL+GCSLLTTEGLESVILQWKELQSLRVVSCKNIKES ISPALSSLFSI KDLKWRP 
Sbjct: 423 FLSLKGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESSISPALSSLFSIFKDLKWRPH 482

Query: 484 TKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           TKSLLPS+LI THMGKKGGRFFKKTWDIKVLPGVH H  TR+T
Sbjct: 483 TKSLLPSTLIHTHMGKKGGRFFKKTWDIKVLPGVHGHTSTRKT 524

BLAST of MS000294 vs. ExPASy TrEMBL
Match: A0A0A0KZ56 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022330 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.4e-271
Identity = 463/526 (88.02%), Postives = 491/526 (93.35%), Query Frame = 0

Query: 1   MGYAHQGPQAVLFEAERRSSISGSVPSPQTNPSSPV-KKRAPRWSDVWLKNTKSLENVIV 60
           MG+A+   Q+VL E ERR S   SVPSPQTNP+SPV KKR PRWSDVWLKNT SLENVI+
Sbjct: 1   MGFANHRQQSVLLEGERRRSSISSVPSPQTNPNSPVKKKRTPRWSDVWLKNTNSLENVIL 60

Query: 61  AMQLQSLCKDSR-STPSSKTKPLITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVC 120
           AM+LQS  KDS   TP+SKTKPL+ NFCK+DRT+LLSDELLL+ILSKLPDSQRNSNFLVC
Sbjct: 61  AMKLQSFSKDSHIPTPNSKTKPLLANFCKIDRTLLLSDELLLRILSKLPDSQRNSNFLVC 120

Query: 121 KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLS 180
           KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNS ILLSNR+LS
Sbjct: 121 KRWLNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSGILLSNRILS 180

Query: 181 MHIDSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECAT 240
           MH+DSWFLP+PGVGEENILD+MVID+GLK LASGCPNLRKLAFIGGSE+GLLSVAEEC T
Sbjct: 181 MHVDSWFLPVPGVGEENILDDMVIDKGLKTLASGCPNLRKLAFIGGSEMGLLSVAEECET 240

Query: 241 LQELELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300
           LQELELH+CNDNLLRGIAACENLQILKL+GNVDGLYSSVVTDIGLTILAQGCKRLVKLEL
Sbjct: 241 LQELELHKCNDNLLRGIAACENLQILKLIGNVDGLYSSVVTDIGLTILAQGCKRLVKLEL 300

Query: 301 NGCEGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPG 360
           NGCEGSFDGIKAIGQCCQMLEELT+CDHRMD+GWLA LSYCENLKTLRIMSCR+IDPNPG
Sbjct: 301 NGCEGSFDGIKAIGQCCQMLEELTICDHRMDDGWLAALSYCENLKTLRIMSCRKIDPNPG 360

Query: 361 PDEYLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCW 420
           PDEYLSPCPALERLHL NCQLR++RSAKALFVTCGAAREIF  DCWG+VD IFSFASH W
Sbjct: 361 PDEYLSPCPALERLHLHNCQLRERRSAKALFVTCGAAREIFFSDCWGLVDGIFSFASHFW 420

Query: 421 RVRFLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKW 480
           RV+FLSLEGCSLLTT+GLESVILQW ELQSLRV SCKNIKES ISPALSSLFSI KDLKW
Sbjct: 421 RVKFLSLEGCSLLTTQGLESVILQWNELQSLRVESCKNIKESSISPALSSLFSIFKDLKW 480

Query: 481 RPDTKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           RPDTKSLL SSL GTHMGKKGGRFFKKTWD+KVLPGVHDH  TRQT
Sbjct: 481 RPDTKSLLSSSLTGTHMGKKGGRFFKKTWDMKVLPGVHDHTSTRQT 526

BLAST of MS000294 vs. ExPASy TrEMBL
Match: A0A6J1HUD6 (F-box protein At5g51380-like OS=Cucurbita maxima OX=3661 GN=LOC111467523 PE=4 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 1.8e-269
Identity = 465/523 (88.91%), Postives = 491/523 (93.88%), Query Frame = 0

Query: 4   AHQGPQAVLFEAERRSSISGSVPSPQTNPSSPVKKRAPRWSDVWLKNTKSLENVIVAMQL 63
           AH   Q+VLFEAERRS IS SVPSPQTNP+SP KKR PRWSDVWLK+TKSLENVIVAMQL
Sbjct: 3   AHGRAQSVLFEAERRSFIS-SVPSPQTNPNSPGKKRTPRWSDVWLKDTKSLENVIVAMQL 62

Query: 64  QSLCKDSR-STPSSKTKP-LITNFCKLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRW 123
           QS CKDS+  T +SKT P LI NF  +DRT+LLSDELLL+IL+KLPDSQRNSNFLVCKRW
Sbjct: 63  QSSCKDSQIPTLNSKTNPLLIANFSNIDRTLLLSDELLLRILTKLPDSQRNSNFLVCKRW 122

Query: 124 LNLQGRLVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHI 183
           LNLQGR+VRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLM SRNS ILLSNR+ SMH+
Sbjct: 123 LNLQGRIVRSLRVMDLNFLLSGRLILRFPNLNRVDLVSGSLMCSRNSGILLSNRIHSMHV 182

Query: 184 DSWFLPIPGVGEENILDNMVIDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQE 243
           DSWFLPIPGVGEENILDNMVIDRGLKALA GCPNLRKLAFIGGSELGLLSVAEEC TLQE
Sbjct: 183 DSWFLPIPGVGEENILDNMVIDRGLKALADGCPNLRKLAFIGGSELGLLSVAEECETLQE 242

Query: 244 LELHRCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGC 303
           LELH+CNDNLLRGIAACENLQILKLVGNVDGLYSSVVTD+GLTILAQGCKRLVKLELNGC
Sbjct: 243 LELHKCNDNLLRGIAACENLQILKLVGNVDGLYSSVVTDVGLTILAQGCKRLVKLELNGC 302

Query: 304 EGSFDGIKAIGQCCQMLEELTLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDE 363
           EGSFDGIKAIGQCCQMLEELT+CDHRMDNGWLAGLSY ENLKTLRI+SCRRIDPNPGPDE
Sbjct: 303 EGSFDGIKAIGQCCQMLEELTICDHRMDNGWLAGLSYSENLKTLRIVSCRRIDPNPGPDE 362

Query: 364 YLSPCPALERLHLQNCQLRDKRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVR 423
           YLSPCPALERLHLQNCQLRDK+SAKALF+TC AAREIFI DCWG+ +D+FSFASHCWRV+
Sbjct: 363 YLSPCPALERLHLQNCQLRDKKSAKALFITCSAAREIFISDCWGLANDLFSFASHCWRVK 422

Query: 424 FLSLEGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPD 483
           FLSL+GCSLLTTEGLESVILQWKELQSLRVVSCKNIKES I+PALSSLFSI KDLKWRPD
Sbjct: 423 FLSLKGCSLLTTEGLESVILQWKELQSLRVVSCKNIKESSITPALSSLFSIFKDLKWRPD 482

Query: 484 TKSLLPSSLIGTHMGKKGGRFFKKTWDIKVLPGVHDHAITRQT 525
           TKSLLPS+LI THMGKKGGRFFKKTWDIKVLPGVH H  T +T
Sbjct: 483 TKSLLPSTLIDTHMGKKGGRFFKKTWDIKVLPGVHGHTSTLKT 524

BLAST of MS000294 vs. TAIR 10
Match: AT5G51380.1 (RNI-like superfamily protein )

HSP 1 Score: 560.1 bits (1442), Expect = 1.9e-159
Identity = 282/476 (59.24%), Postives = 355/476 (74.58%), Query Frame = 0

Query: 30  TNPSSPVKKRAPRWSDVWLKNTKSLENVIVAMQLQSLCKDSRSTPSSKTKPLITNFCKLD 89
           T+P SP+++R   W+  WL +  +     V+  +Q+       +P S+ K L ++F  +D
Sbjct: 9   TSPKSPLRRRRSSWTGTWLNHQTTSFKQAVSAVIQA------QSPRSRFKSLSSDFSDVD 68

Query: 90  RTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLILRF 149
           RT+ LSD LLLKIL KLP+SQ     LVCKRWL++QGR +RS++V D  FLLSGRL+ RF
Sbjct: 69  RTLSLSDSLLLKILEKLPESQNEDVSLVCKRWLSVQGRRLRSMKVFDWEFLLSGRLVSRF 128

Query: 150 PNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDSWFLPIPGVGEENILDNMVIDRGLKAL 209
           P L  VDLV+     S NS ILL +  +S H+ +         EE++LDN ++D+GL+ L
Sbjct: 129 PKLTSVDLVNACFNPSSNSGILLCHTSISFHVSTDSSLNLNFVEESLLDNEMVDKGLRVL 188

Query: 210 ASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENLQILKLVGN 269
             G  +L KL  I  +ELGLLS+AE+C+ LQELELH+C+DNLLRGIAACENL+ L+LVG+
Sbjct: 189 GRGSFDLIKLVVINATELGLLSLAEDCSDLQELELHKCSDNLLRGIAACENLRGLRLVGS 248

Query: 270 VDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEELTLCDHRMD 329
           VDGLYSS V+DIGLTILAQGCKRLVKLEL+GCEGSFDGIKAIGQCC++LEEL++CDHRMD
Sbjct: 249 VDGLYSSSVSDIGLTILAQGCKRLVKLELSGCEGSFDGIKAIGQCCEVLEELSICDHRMD 308

Query: 330 NGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQNCQLRDKRSAKALF 389
           +GW+A LSY E+LKTL I SCR+ID +PGP + L  CPALE L L+ C L DK   +ALF
Sbjct: 309 DGWIAALSYFESLKTLLISSCRKIDSSPGPGKLLGSCPALESLQLRRCCLNDKEGMRALF 368

Query: 390 VTCGAAREIFIRDCWGVVDDIFSFASHCWRVRFLSLEGCSLLTTEGLESVILQWKELQSL 449
             C    ++ I+DCWG+ DD FS A    RVRFLSLEGCS+LTT GLESVIL W+EL+S+
Sbjct: 369 KVCDGVTKVNIQDCWGLDDDSFSLAKAFRRVRFLSLEGCSILTTSGLESVILHWEELESM 428

Query: 450 RVVSCKNIKESRISPALSSLFSILKDLKWRPDTKSLLPSSLIGTHMGKKGGRFFKK 506
           RVVSCKNIK+S IS ALSSLFS+LK+L WRPDT+S L SSL GT +GK+G +FFKK
Sbjct: 429 RVVSCKNIKDSEISAALSSLFSLLKELTWRPDTRSHLSSSLEGTGIGKRGSKFFKK 478

BLAST of MS000294 vs. TAIR 10
Match: AT5G07670.1 (RNI-like superfamily protein )

HSP 1 Score: 556.2 bits (1432), Expect = 2.8e-158
Identity = 287/481 (59.67%), Postives = 364/481 (75.68%), Query Frame = 0

Query: 29  QTNPSSPVKKRAPRWSDVWLKNTKSLEN--VIVAMQLQSLCKDSRSTPSSKTKPLITNFC 88
           +++P SP+KKR   WS++W+ +   L +  + +A + QSL     + P SK+K L+ +F 
Sbjct: 8   ESSPVSPLKKRRASWSELWVNHHHLLSSSPLDLAAKFQSL-----TPPISKSKTLLPDF- 67

Query: 89  KLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLI 148
               T+LL D +L++++ K+P+SQR +  LVCKRW  L GRLVRS +V D  FL SGRLI
Sbjct: 68  ----TLLLPDLILIRVIQKIPNSQRKNLSLVCKRWFRLHGRLVRSFKVSDWEFLSSGRLI 127

Query: 149 LRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDSWFLPIPGVGEENILDNMVIDRGL 208
            RFPNL  VDLVSG L+S  N  IL+++R++S  +           EEN+L   +++RGL
Sbjct: 128 SRFPNLETVDLVSGCLISPPNLGILVNHRIVSFTVGVGSYQSWSFFEENLLSVELVERGL 187

Query: 209 KALASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENLQILKL 268
           KALA GC NLRKL     SELGLL+VAEEC+ LQELELH+C+D++L GI A ENLQIL+L
Sbjct: 188 KALAGGCSNLRKLVVTNTSELGLLNVAEECSRLQELELHKCSDSVLLGIGAFENLQILRL 247

Query: 269 VGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEELTLCDH 328
           VGNVDGLY+S+V+DIGL ILAQGCKRLVKLEL GCEG FDGIK IG+CCQMLEELT+CD+
Sbjct: 248 VGNVDGLYNSLVSDIGLMILAQGCKRLVKLELVGCEGGFDGIKEIGECCQMLEELTVCDN 307

Query: 329 RMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLS-PCPALERLHLQNCQLRDKRSA 388
           +M++GWL GL YCENLKTL+++SC++ID +  PDE LS  CPALERL L+ CQLRDK + 
Sbjct: 308 KMESGWLGGLRYCENLKTLKLVSCKKIDND--PDESLSCCCPALERLQLEKCQLRDKNTV 367

Query: 389 KALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVRFLSLEGCSLLTTEGLESVILQWKE 448
           KALF  C AAREI  +DCWG+ +DIFS A    RV+ L LEGCSLLTT GLESVIL W E
Sbjct: 368 KALFKMCEAAREIVFQDCWGLDNDIFSLAMAFGRVKLLYLEGCSLLTTSGLESVILHWHE 427

Query: 449 LQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPDTKSLLPSSLIGTHMGKKGGRFFKK 507
           L+ L+VVSCKNIK+S +SP+LS+LFS L +L+WRPDT+S L SSL GT +G+KGG+FFKK
Sbjct: 428 LEHLKVVSCKNIKDSEVSPSLSALFSALVELQWRPDTRSHLSSSLTGTGIGEKGGKFFKK 476

BLAST of MS000294 vs. TAIR 10
Match: AT5G51370.2 (RNI-like superfamily protein )

HSP 1 Score: 521.2 bits (1341), Expect = 9.9e-148
Identity = 264/425 (62.12%), Postives = 327/425 (76.94%), Query Frame = 0

Query: 87  KLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLI 146
           ++D T+ LSD LLLKI+ KLP+SQ N   LVCKRWLNLQG+ +RSL+++D +FLLS RL 
Sbjct: 25  EIDSTLSLSDSLLLKIIEKLPESQSNDVSLVCKRWLNLQGQRLRSLKLLDFDFLLSERLT 84

Query: 147 LRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDS----W-FLPIPGVGEENILDNMV 206
            RFPNL  VDLV+  +    NS IL  ++ +S H+ S    W FL      EEN+L + V
Sbjct: 85  TRFPNLTHVDLVNACMNPRVNSGILFCHKSISFHLSSDSSNWEFL------EENLLHSDV 144

Query: 207 IDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENL 266
           IDRGL+ L+    +L  L  I  SELGLLS+A +C+ LQELELH+CNDNLL GIAAC+NL
Sbjct: 145 IDRGLRILSRESFDLLNLKVINASELGLLSLAGDCSDLQELELHKCNDNLLHGIAACKNL 204

Query: 267 QILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEEL 326
           + L+LVG+VDGLYSS V+DIGLT LAQGC+ LVKLEL+GCEGSFDGIKAIGQCC++LEEL
Sbjct: 205 KGLRLVGSVDGLYSSSVSDIGLTFLAQGCRSLVKLELSGCEGSFDGIKAIGQCCEVLEEL 264

Query: 327 TLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQNCQLRD 386
           ++CDHRMD+GW+A LSY E+LK LRI SCR+ID +PGP++ L  CPA+E L L+ C L D
Sbjct: 265 SICDHRMDDGWIAALSYFESLKILRISSCRKIDASPGPEKLLRSCPAMESLQLKRCCLND 324

Query: 387 KRSAKALFVTCGAAREIFIRDCWGVVDDIFSFASHCWRVRFLSLEGCSLLTTEGLESVIL 446
           K   KALF  C  A E+ I+DCWG+ DD FS A    RVRFLSLEGCS+LT+ GLESVIL
Sbjct: 325 KEGIKALFKVCDGATEVNIQDCWGLSDDCFSLAKAFRRVRFLSLEGCSVLTSGGLESVIL 384

Query: 447 QWKELQSLRVVSCKNIKESRISPALSSLFSILKDLKWRPDTKSLLPSSLIGTHMGKKGGR 506
            W+EL+S+RVVSCK+IK+S ISPALSSLFS+LK+L WRPDT+S L SSL G  +G +G +
Sbjct: 385 HWEELESMRVVSCKSIKDSEISPALSSLFSLLKELTWRPDTRSHLSSSLEGAGIGIRGSK 443

BLAST of MS000294 vs. TAIR 10
Match: AT5G51370.1 (RNI-like superfamily protein )

HSP 1 Score: 398.7 bits (1023), Expect = 7.4e-111
Identity = 201/333 (60.36%), Postives = 250/333 (75.08%), Query Frame = 0

Query: 87  KLDRTMLLSDELLLKILSKLPDSQRNSNFLVCKRWLNLQGRLVRSLRVMDLNFLLSGRLI 146
           ++D T+ LSD LLLKI+ KLP+SQ N   LVCKRWLNLQG+ +RSL+++D +FLLS RL 
Sbjct: 25  EIDSTLSLSDSLLLKIIEKLPESQSNDVSLVCKRWLNLQGQRLRSLKLLDFDFLLSERLT 84

Query: 147 LRFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDS----W-FLPIPGVGEENILDNMV 206
            RFPNL  VDLV+  +    NS IL  ++ +S H+ S    W FL      EEN+L + V
Sbjct: 85  TRFPNLTHVDLVNACMNPRVNSGILFCHKSISFHLSSDSSNWEFL------EENLLHSDV 144

Query: 207 IDRGLKALASGCPNLRKLAFIGGSELGLLSVAEECATLQELELHRCNDNLLRGIAACENL 266
           IDRGL+ L+    +L  L  I  SELGLLS+A +C+ LQELELH+CNDNLL GIAAC+NL
Sbjct: 145 IDRGLRILSRESFDLLNLKVINASELGLLSLAGDCSDLQELELHKCNDNLLHGIAACKNL 204

Query: 267 QILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFDGIKAIGQCCQMLEEL 326
           + L+LVG+VDGLYSS V+DIGLT LAQGC+ LVKLEL+GCEGSFDGIKAIGQCC++LEEL
Sbjct: 205 KGLRLVGSVDGLYSSSVSDIGLTFLAQGCRSLVKLELSGCEGSFDGIKAIGQCCEVLEEL 264

Query: 327 TLCDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQNCQLRD 386
           ++CDHRMD+GW+A LSY E+LK LRI SCR+ID +PGP++ L  CPA+E L L+ C L D
Sbjct: 265 SICDHRMDDGWIAALSYFESLKILRISSCRKIDASPGPEKLLRSCPAMESLQLKRCCLND 324

Query: 387 KRSAKALFVTCGAAREIFIRDCWGVVDDIFSFA 415
           K   KALF  C  A E+ I+DCWG+ DD FS A
Sbjct: 325 KEGIKALFKVCDGATEVNIQDCWGLSDDCFSLA 351

BLAST of MS000294 vs. TAIR 10
Match: AT4G15475.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 108.6 bits (270), Expect = 1.5e-23
Identity = 104/388 (26.80%), Postives = 176/388 (45.36%), Query Frame = 0

Query: 94  LSDELLLKILSKLPDS-QRNSNFLVCKRWLNLQGRLVRSLRV-----MDLNFLLSGRLIL 153
           L +EL+L+I  +L     R++  LVCKRWL+L+     +LR+      D    L  R  L
Sbjct: 11  LPEELILEIFRRLESKPNRDACSLVCKRWLSLERFSRTTLRIGASFSPDDFISLLSRRFL 70

Query: 154 RFPNLNRVDLVSGSLMSSRNSSILLSNRVLSMHIDSWFLPIPG---VGEENILDNMVIDR 213
              +++  + +S SL S   S      R  S    S    +      G EN+  + + D 
Sbjct: 71  YITSIHVDERISVSLPSLSPSPKRKRGRDSSSPSSSKRKKLTDKTHSGAENVESSSLTDT 130

Query: 214 GLKALASGCPNLRKLAFI---GGSELGLLSVAEECATLQELELHRC--NDNLLRGIAA-C 273
           GL ALA+G P +  L+ I     S +GL S+A++C +L+ L+L  C   D  L  +   C
Sbjct: 131 GLTALANGFPRIENLSLIWCPNVSSVGLCSLAQKCTSLKSLDLQGCYVGDQGLAAVGKFC 190

Query: 274 ENLQILKLVGNVDGLYSSVVTDIGLTILAQGCKRLVKLELNGCEGSFD--GIKAIGQCCQ 333
           + L+ L L       +   +TD+G+  L  GC + +K              ++A+G  C+
Sbjct: 191 KQLEELNL------RFCEGLTDVGVIDLVVGCSKSLKSIGVAASAKITDLSLEAVGSHCK 250

Query: 334 MLEELTL-CDHRMDNGWLAGLSYCENLKTLRIMSCRRIDPNPGPDEYLSPCPALERLHLQ 393
           +LE L L  ++  D G +A    C  LK L++      D        L  C +LERL L 
Sbjct: 251 LLEVLYLDSEYIHDKGLIAVAQGCHRLKNLKLQCVSVTDVAFAAVGEL--CTSLERLALY 310

Query: 394 NCQLRDKRSAKALFVTCGAAREIFIRDCWGV-VDDIFSFASHCWRVRFLSLEGCSLLTTE 453
           + Q    +  +A+       +++ + DC+ V    + + A  C  +  + + GC  + T 
Sbjct: 311 SFQHFTDKGMRAIGKGSKKLKDLTLSDCYFVSCKGLEAIAHGCKELERVEINGCHNIGTR 370

Query: 454 GLESVILQWKELQSLRVVSCKNIKESRI 463
           G+E++      L+ L ++ C+ I  S +
Sbjct: 371 GIEAIGKSCPRLKELALLYCQRIGNSAL 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141321.12.8e-30198.85F-box protein At5g51380-like [Momordica charantia][more]
XP_038879973.12.1e-27790.49F-box protein At5g51380-like [Benincasa hispida][more]
XP_008453917.12.2e-27489.16PREDICTED: F-box protein At5g51380-like [Cucumis melo][more]
XP_023528939.11.2e-27289.67F-box protein At5g51370-like [Cucurbita pepo subsp. pepo][more]
XP_022942171.17.8e-27289.48F-box protein At5g51370-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9FGN32.7e-15859.24F-box protein At5g51380 OS=Arabidopsis thaliana OX=3702 GN=At5g51380 PE=2 SV=1[more]
Q8VYT53.9e-15759.67F-box protein At5g07670 OS=Arabidopsis thaliana OX=3702 GN=At5g07670 PE=2 SV=1[more]
Q9FGN41.4e-14662.12F-box protein At5g51370 OS=Arabidopsis thaliana OX=3702 GN=At5g51370 PE=2 SV=1[more]
Q9C5D22.2e-2226.80F-box/LRR-repeat protein 4 OS=Arabidopsis thaliana OX=3702 GN=FBL4 PE=2 SV=1[more]
Q5BJ298.8e-1625.93F-box/LRR-repeat protein 7 OS=Mus musculus OX=10090 GN=Fbxl7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CIT91.3e-30198.85F-box protein At5g51380-like OS=Momordica charantia OX=3673 GN=LOC111011743 PE=4... [more]
A0A1S3BYL51.1e-27489.16F-box protein At5g51380-like OS=Cucumis melo OX=3656 GN=LOC103494490 PE=4 SV=1[more]
A0A6J1FN363.8e-27289.48F-box protein At5g51370-like OS=Cucurbita moschata OX=3662 GN=LOC111447311 PE=4 ... [more]
A0A0A0KZ561.4e-27188.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022330 PE=4 SV=1[more]
A0A6J1HUD61.8e-26988.91F-box protein At5g51380-like OS=Cucurbita maxima OX=3661 GN=LOC111467523 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G51380.11.9e-15959.24RNI-like superfamily protein [more]
AT5G07670.12.8e-15859.67RNI-like superfamily protein [more]
AT5G51370.29.9e-14862.12RNI-like superfamily protein [more]
AT5G51370.17.4e-11160.36RNI-like superfamily protein [more]
AT4G15475.11.5e-2326.80F-box/RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 225..471
e-value: 1.9E-32
score: 114.2
NoneNo IPR availableGENE3D1.20.1280.50coord: 91..127
e-value: 9.0E-7
score: 30.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..37
NoneNo IPR availablePANTHERPTHR13318:SF124F-BOX-LIKE PROTEINcoord: 79..484
NoneNo IPR availablePANTHERPTHR13318UNCHARACTERIZEDcoord: 79..484
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 138..269
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 274..469
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 76..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000294.1MS000294.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding