MS000289 (gene) Bitter gourd (TR) v1

Overview


Name	MS000289
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA-directed RNA polymerase III subunit rpc4-like
Location	scaffold44: 637526 .. 638880 (+)
RNA-Seq Expression	MS000289
Synteny	MS000289

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTCAGTCCAAGTTGCATTTGGTCCCGGAGCAGCATCAGCATCATCTTCCATAAGAACATATGGTGTTCCCAAAGTGGGGAACAGTAGTAGAAAAAATAACATTGAGCCTGAAATTTCTGACGATGAGGAATCTATCTTGCCTCTCCCTGTGGATGTTGAAGAAGATGGGATGATTTTCGATCAAAAAAGCGAAGATGGAACACCCGAATCCGCCATGGCTACCAATGCTGTGGTACTAACCAAGGCCAAGAAGGATTACAGAGAACCTTGGGTAATCTCTCCCTCTCTTTCTCTATCTCGCCTTTGCGCACACACTTATAGATTAACATCCCGATTTCGAGATGCTTTTCAGGATTACCATCATTCTTACTATCCCAATACGCTTCCTTTACGGAAGCCTTACTCCGGACACCCAGGTATGTCTCTATTTCTTTGTTCACTATTAGTTTTTGTTTGTGAAAAGTGATCTCTAGTGCTCAAAATCAACCTTGAAAACAAAAGCAGACTCCCAATAACTAATGACTATGGATGATTAATGGTGGATGAATCCTTGCTTATTTATGTTGCAATGAACATTTTACCTTGGTGGTGGATCATTCTGGCTTTGCTCTGTAATTGCTAACTTTCCAGCTTCTGATTTTAGAACTACTTGATAAAGCTGAATTCGGGCTGGATGCACCGGATAGGGAGTATGACGAGAACGCTGCAATACCTGCATTAGATCTAGAATTGCTGGTTGGTATTTGGATTGATAACTGTGGATTATTTCATATACTTTTTGGTAGTCTTTCTTCATTTAGAAACTAGTTATAATATGGTGGCCATTTGAAGCAGGAGGAAAAGGAGGAGAAGACTATGTACTTTTTTCAGCTTCCTGCCTGTCTTCCTTTACCCAAACAATCATCTACTGCAACAAGGAAGGAGAAAGTAGGAAGTTCGAGATCTTCGAACAGCAAAGGCACGTCAGAGCTCGGCGATTTGAAGAAATTGCCTGGTGGATGTATGGGGAAACTACTGATATACAAGAGTGGAGCTGTTAAGTTGAGGCTTGGAGAAACTCTTTACGACGTGAGAAATATGTCATTGGCTTCCTATAAATCGAAATAGAGCATCAAGGAGTTCGTTTTTTGTTATTGTTCTTGATGTTTGGGGATTAGTTAGACCTTGTAATTTCTTTTTGGTTTCTCTTTTTGGGGCTTTTTGTTGCAGGTTTCTTCTGGTTCAAACTGCAGCTTTCTTCAACATGTTGTGGCAATCAACACCGAAGAGGGACACTGCTGTGATCTCGGAGATGTCGGAAAACGAGTCGTTGTGACGCCGGATATTAGTTCCCTGCTGAATGCAGTGACTGAC

mRNA sequence

GCTTCAGTCCAAGTTGCATTTGGTCCCGGAGCAGCATCAGCATCATCTTCCATAAGAACATATGGTGTTCCCAAAGTGGGGAACAGTAGTAGAAAAAATAACATTGAGCCTGAAATTTCTGACGATGAGGAATCTATCTTGCCTCTCCCTGTGGATGTTGAAGAAGATGGGATGATTTTCGATCAAAAAAGCGAAGATGGAACACCCGAATCCGCCATGGCTACCAATGCTGTGGTACTAACCAAGGCCAAGAAGGATTACAGAGAACCTTGGGATTACCATCATTCTTACTATCCCAATACGCTTCCTTTACGGAAGCCTTACTCCGGACACCCAGAACTACTTGATAAAGCTGAATTCGGGCTGGATGCACCGGATAGGGAGTATGACGAGAACGCTGCAATACCTGCATTAGATCTAGAATTGCTGGAGGAAAAGGAGGAGAAGACTATGTACTTTTTTCAGCTTCCTGCCTGTCTTCCTTTACCCAAACAATCATCTACTGCAACAAGGAAGGAGAAAGTAGGAAGTTCGAGATCTTCGAACAGCAAAGGCACGTCAGAGCTCGGCGATTTGAAGAAATTGCCTGGTGGATGTATGGGGAAACTACTGATATACAAGAGTGGAGCTGTTAAGTTGAGGCTTGGAGAAACTCTTTACGACGTTTCTTCTGGTTCAAACTGCAGCTTTCTTCAACATGTTGTGGCAATCAACACCGAAGAGGGACACTGCTGTGATCTCGGAGATGTCGGAAAACGAGTCGTTGTGACGCCGGATATTAGTTCCCTGCTGAATGCAGTGACTGAC

Coding sequence (CDS)

GCTTCAGTCCAAGTTGCATTTGGTCCCGGAGCAGCATCAGCATCATCTTCCATAAGAACATATGGTGTTCCCAAAGTGGGGAACAGTAGTAGAAAAAATAACATTGAGCCTGAAATTTCTGACGATGAGGAATCTATCTTGCCTCTCCCTGTGGATGTTGAAGAAGATGGGATGATTTTCGATCAAAAAAGCGAAGATGGAACACCCGAATCCGCCATGGCTACCAATGCTGTGGTACTAACCAAGGCCAAGAAGGATTACAGAGAACCTTGGGATTACCATCATTCTTACTATCCCAATACGCTTCCTTTACGGAAGCCTTACTCCGGACACCCAGAACTACTTGATAAAGCTGAATTCGGGCTGGATGCACCGGATAGGGAGTATGACGAGAACGCTGCAATACCTGCATTAGATCTAGAATTGCTGGAGGAAAAGGAGGAGAAGACTATGTACTTTTTTCAGCTTCCTGCCTGTCTTCCTTTACCCAAACAATCATCTACTGCAACAAGGAAGGAGAAAGTAGGAAGTTCGAGATCTTCGAACAGCAAAGGCACGTCAGAGCTCGGCGATTTGAAGAAATTGCCTGGTGGATGTATGGGGAAACTACTGATATACAAGAGTGGAGCTGTTAAGTTGAGGCTTGGAGAAACTCTTTACGACGTTTCTTCTGGTTCAAACTGCAGCTTTCTTCAACATGTTGTGGCAATCAACACCGAAGAGGGACACTGCTGTGATCTCGGAGATGTCGGAAAACGAGTCGTTGTGACGCCGGATATTAGTTCCCTGCTGAATGCAGTGACTGAC

Protein sequence

ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSSNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD

Homology

[Close]

BLAST of MS000289 vs. NCBI nr
Match: XP_022141051.1 (DNA-directed RNA polymerase III subunit RPC4-like isoform X2 [Momordica charantia])

HSP 1 Score: 526.6 bits (1355), Expect = 1.3e-145
Identity = 267/268 (99.63%), Postives = 267/268 (99.63%), Query Frame = 0


Query: 2   SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 61
           SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD
Sbjct: 67  SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 126

Query: 62  QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 121
           QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG
Sbjct: 127 QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 186

Query: 122 LDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSS 181
           LDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSS
Sbjct: 187 LDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSS 246

Query: 182 NSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEE 241
           NSKGTSELGDLKKLPGG MGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEE
Sbjct: 247 NSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEE 306

Query: 242 GHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           GHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 307 GHCCDLGDVGKRVVVTPDISSLLNAVTD 334

[Close]

BLAST of MS000289 vs. NCBI nr
Match: XP_022141050.1 (DNA-directed RNA polymerase III subunit RPC4-like isoform X1 [Momordica charantia])

HSP 1 Score: 521.9 bits (1343), Expect = 3.2e-144
Identity = 267/269 (99.26%), Postives = 267/269 (99.26%), Query Frame = 0


Query: 2   SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 61
           SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD
Sbjct: 67  SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 126

Query: 62  QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 121
           QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG
Sbjct: 127 QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 186

Query: 122 LDAPDREYDENAAIPALDLELL-EEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 181
           LDAPDREYDENAAIPALDLELL EEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS
Sbjct: 187 LDAPDREYDENAAIPALDLELLQEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 246

Query: 182 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 241
           SNSKGTSELGDLKKLPGG MGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE
Sbjct: 247 SNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 306

Query: 242 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           EGHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 307 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 335

[Close]

BLAST of MS000289 vs. NCBI nr
Match: XP_038879339.1 (DNA-directed RNA polymerase III subunit rpc4-like [Benincasa hispida])

HSP 1 Score: 406.4 bits (1043), Expect = 2.0e-109
Identity = 204/269 (75.84%), Postives = 237/269 (88.10%), Query Frame = 0


Query: 1   ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIF 60
           +SVQVAFGPGAAS SSSIRTYGVPKV NSSRKN+IEP++ +DEE ILPLP+DVEE+G IF
Sbjct: 65  SSVQVAFGPGAASTSSSIRTYGVPKVENSSRKNDIEPDVDEDEEFILPLPMDVEENGKIF 124

Query: 61  DQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEF 120
           D+K++DG  ES   +++ ++TK KKDY+EPWDYH+S YP TLP+R PYSG PE LD+AEF
Sbjct: 125 DKKTKDGIAES---SSSAIVTKTKKDYKEPWDYHNSNYPTTLPIRMPYSGDPEQLDEAEF 184

Query: 121 GLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 180
           G DA DREYDE++ IPALDL LL+EK E T YFFQLPA LPLPKQSSTAT KEKVG+ RS
Sbjct: 185 GQDATDREYDEDSVIPALDLGLLDEKTENTKYFFQLPARLPLPKQSSTATGKEKVGNLRS 244

Query: 181 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 240
           S S G+SELGDLKKL  GCMGKLLIYKSGA+KLRLG+TLYDVSSGS+CSFLQHVVAINT+
Sbjct: 245 SKSMGSSELGDLKKLSAGCMGKLLIYKSGAIKLRLGDTLYDVSSGSDCSFLQHVVAINTK 304

Query: 241 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           EG CCDLGD+GKRV+VTPDISSLLN++T+
Sbjct: 305 EGQCCDLGDIGKRVIVTPDISSLLNSMTN 330

[Close]

BLAST of MS000289 vs. NCBI nr
Match: KAA0044650.1 (DNA-directed RNA polymerase III subunit rpc4-like [Cucumis melo var. makuwa])

HSP 1 Score: 390.2 bits (1001), Expect = 1.5e-104
Identity = 197/269 (73.23%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIF 60
           +SVQVAFGPGA S SSSIRTYGVPKV N SRKN+IEPE+ +DEE +LP+ +DV EDG  F
Sbjct: 67  SSVQVAFGPGAESTSSSIRTYGVPKVENGSRKNDIEPEVDEDEEFVLPVAMDVNEDGKFF 126

Query: 61  DQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEF 120
           D+K++DG  ES   +++ ++TK K+DY+EPWDY +SYYP TLPLR PYSG PE LD+AEF
Sbjct: 127 DKKTKDGIAES---SSSAMVTKTKRDYKEPWDYQNSYYPTTLPLRMPYSGDPERLDEAEF 186

Query: 121 GLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 180
           G DA +REYDEN+ IPALDL L +E  E T YFFQLPA LPLPKQSSTAT KEKVG+SRS
Sbjct: 187 GQDAMNREYDENSVIPALDLGLQDENTENTKYFFQLPARLPLPKQSSTATGKEKVGNSRS 246

Query: 181 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 240
           SNS  +S+L DLKKL  GCMGKLLIYKSGA+KLRLG+ LYDVSSGS+CSFLQHVVAINTE
Sbjct: 247 SNSTSSSDLDDLKKLSAGCMGKLLIYKSGAIKLRLGDILYDVSSGSDCSFLQHVVAINTE 306

Query: 241 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           +G CCDLGD+GKRVVVTPDISSLLN+VT+
Sbjct: 307 KGQCCDLGDIGKRVVVTPDISSLLNSVTN 332

[Close]

BLAST of MS000289 vs. NCBI nr
Match: XP_004146914.1 (DNA-directed RNA polymerase III subunit rpc4 [Cucumis sativus] >KGN53151.1 hypothetical protein Csa_015254 [Cucumis sativus])

HSP 1 Score: 389.0 bits (998), Expect = 3.2e-104
Identity = 198/269 (73.61%), Postives = 226/269 (84.01%), Query Frame = 0


Query: 1   ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIF 60
           +SVQVAFGPGA S SSSIRTYGVPKV N SRKN+IEPE+ +DEE +LP+  DV EDG  F
Sbjct: 67  SSVQVAFGPGAESTSSSIRTYGVPKVENGSRKNDIEPEVDEDEEFVLPVARDVNEDGKYF 126

Query: 61  DQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEF 120
           D+K++DG  ES   +++ + TK K+DY+EPWDY +SYYP TLPLR PYSG PE LD+AEF
Sbjct: 127 DKKTKDGITES---SSSAMETKTKRDYKEPWDYQNSYYPTTLPLRMPYSGDPERLDEAEF 186

Query: 121 GLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 180
           G D  +REYDEN+ IPALDL LL+E  E T YFFQLPA LPLPKQSSTAT KEKVG+SRS
Sbjct: 187 GQDVMNREYDENSVIPALDLGLLDENTESTKYFFQLPARLPLPKQSSTATGKEKVGNSRS 246

Query: 181 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 240
           SNS  +S+L DLKKL  GCMGKLLIYKSGA+KLRLG+ LYDVSSGSNCSFLQHVVAINTE
Sbjct: 247 SNSTSSSDLDDLKKLSAGCMGKLLIYKSGAIKLRLGDILYDVSSGSNCSFLQHVVAINTE 306

Query: 241 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           EG CCDLGD+G RVVVTPDISSLLN+VT+
Sbjct: 307 EGQCCDLGDIGNRVVVTPDISSLLNSVTN 332

[Close]

BLAST of MS000289 vs. ExPASy Swiss-Prot
Match: P05423 (DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 1.5e-08
Identity = 54/169 (31.95%), Postives = 80/169 (47.34%), Query Frame = 0


Query: 116 DKAEFGLDAPDREYDENAAIP--ALDLELLEE---KEEKTMYFFQLPACLP--LPKQSST 175
           ++ E  + AP +   +   +P      ELL E    +E+ + F QLP  LP   P Q   
Sbjct: 226 EEEEAKMKAPPKAARKTPGLPKDVSVAELLRELSLTKEEELLFLQLPDTLPGQPPTQDIK 285

Query: 176 ATRKEKVG---------SSRSSNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETL 235
             + E  G           +   +K       L  L  G +GKLLI KSG V+L LG+  
Sbjct: 286 PIKTEVQGEDGQVVLIKQEKDREAKLAENACTLADLTEGQVGKLLIRKSGRVQLLLGKVT 345

Query: 236 YDVSSGSNCSFLQHVVAI---NTEEGHCCDLGDVGKRVVVTPDISSLLN 266
            DV+ G+ CSFLQ +V++   ++  G    LG V  ++V +PD  SLL+
Sbjct: 346 LDVTMGTACSFLQELVSVGLGDSRTGEMTVLGHVKHKLVCSPDFESLLD 394

[Close]

BLAST of MS000289 vs. ExPASy Swiss-Prot
Match: Q91WD1 (DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 4.5e-08
Identity = 67/252 (26.59%), Postives = 111/252 (44.05%), Query Frame = 0


Query: 36  EPEISDDEESILPLPVDVEEDGMIFDQKSED--------GTPESAMATNAVVLTKAKKDY 95
           +P + +D  + +P+ + +   G +F ++SE+        G  E  M  + V   K K++ 
Sbjct: 166 DPGLKNDTRN-MPVQLPLAHSGWLFKEESEEPEAKPFSAGPKEEDMEVD-VPAVKVKEEP 225

Query: 96  REPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFGLDAPDREYDENAAIPALDLELLEEKE 155
           R+  +      P     + P  G P+ +  AE                   +L L++++E
Sbjct: 226 RDEEEEAKVKAPPRAARKTP--GLPKDVSVAEL----------------LRELSLMKDEE 285

Query: 156 EKTMYFFQLPACLP--LPKQSSTATRKEKVG---------SSRSSNSKGTSELGDLKKLP 215
              + F QLP  LP   P Q     + E  G           +   ++       L  L 
Sbjct: 286 ---LLFLQLPDTLPGQPPTQDIKPVKTEVQGEDGQMVVIKQEKDREARLAENACTLADLT 345

Query: 216 GGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAI---NTEEGHCCDLGDVGKR 266
            G +GKLLI KSG V+L LG+   DV+ G+ CSFLQ +V++   ++  G    LG V  +
Sbjct: 346 EGQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVGLGDSRTGEMTVLGHVKHK 394

[Close]

BLAST of MS000289 vs. ExPASy Swiss-Prot
Match: Q5E9Z7 (DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-07
Identity = 49/145 (33.79%), Postives = 73/145 (50.34%), Query Frame = 0


Query: 141 ELLEE---KEEKTMYFFQLPACLP-LPKQSSTATRKEKVGSS-------------RSSNS 200
           ELL E    +E+ + F QLP  LP  P        K +V S               +  +
Sbjct: 253 ELLRELSLTQEEELLFLQLPDSLPGQPPTQDIKPIKTEVQSEDGQMVVIKQEKDREARLA 312

Query: 201 KGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAI---NTE 260
           + T  L DL +   G +GKLLI KSG V+L LG+   DV+ G+ CSFLQ +V++   ++ 
Sbjct: 313 ENTCTLADLTE---GQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVGLGDSR 372

Query: 261 EGHCCDLGDVGKRVVVTPDISSLLN 266
            G    LG +  ++V +P+  SLL+
Sbjct: 373 TGDMTVLGHIKHKLVCSPNFESLLD 394

[Close]

BLAST of MS000289 vs. ExPASy Swiss-Prot
Match: Q8T1V7 (DNA-directed RNA polymerase III subunit rpc4 OS=Dictyostelium discoideum OX=44689 GN=polr3d PE=3 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 5.5e-06
Identity = 29/77 (37.66%), Postives = 41/77 (53.25%), Query Frame = 0


Query: 192 LKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEEG-----HCCD 251
           +K++P G +G L IYKSG   L++G   YD+SSG    FL+ V    T+ G      C  
Sbjct: 351 IKEMPSGKLGTLKIYKSGKSILKIGTVEYDISSGDKLKFLEEVQCFQTKGGPDNSPTCYT 410

Query: 252 LGDVGKRVVVTPDISSL 264
           LG   + +V  P IS +
Sbjct: 411 LGIPSQHLVAAPIISQI 427

[Close]

BLAST of MS000289 vs. ExPASy Swiss-Prot
Match: P25441 (DNA-directed RNA polymerase III subunit RPC4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC53 PE=1 SV=2)

HSP 1 Score: 50.1 bits (118), Expect = 4.7e-05
Identity = 38/131 (29.01%), Postives = 69/131 (52.67%), Query Frame = 0


Query: 140 LELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSSNS--------KGTSELGD 199
           L+ +  K E+ M  FQLP  LP  ++ +    KE + +  S  S        K T +   
Sbjct: 294 LKKMNNKPERFM-VFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALS 353

Query: 200 LKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAIN-TEEGHCCD-LGD 259
            ++L  G +G + ++KSG + +++G  + D+  G+  +FLQ V+A++  ++    + LG 
Sbjct: 354 TREL-AGKVGSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGR 413

Query: 260 VGKRVVVTPDI 261
           V  ++VVTP I
Sbjct: 414 VDGKIVVTPQI 422

[Close]

BLAST of MS000289 vs. ExPASy TrEMBL
Match: A0A6J1CHV0 (DNA-directed RNA polymerase III subunit RPC4-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011545 PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 6.3e-146
Identity = 267/268 (99.63%), Postives = 267/268 (99.63%), Query Frame = 0


Query: 2   SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 61
           SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD
Sbjct: 67  SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 126

Query: 62  QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 121
           QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG
Sbjct: 127 QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 186

Query: 122 LDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSS 181
           LDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSS
Sbjct: 187 LDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSS 246

Query: 182 NSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEE 241
           NSKGTSELGDLKKLPGG MGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEE
Sbjct: 247 NSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEE 306

Query: 242 GHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           GHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 307 GHCCDLGDVGKRVVVTPDISSLLNAVTD 334

[Close]

BLAST of MS000289 vs. ExPASy TrEMBL
Match: A0A6J1CGV5 (DNA-directed RNA polymerase III subunit RPC4-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011545 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.5e-144
Identity = 267/269 (99.26%), Postives = 267/269 (99.26%), Query Frame = 0


Query: 2   SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 61
           SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD
Sbjct: 67  SVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFD 126

Query: 62  QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 121
           QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG
Sbjct: 127 QKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFG 186

Query: 122 LDAPDREYDENAAIPALDLELL-EEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 181
           LDAPDREYDENAAIPALDLELL EEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS
Sbjct: 187 LDAPDREYDENAAIPALDLELLQEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 246

Query: 182 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 241
           SNSKGTSELGDLKKLPGG MGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE
Sbjct: 247 SNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 306

Query: 242 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           EGHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 307 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 335

[Close]

BLAST of MS000289 vs. ExPASy TrEMBL
Match: A0A5A7TN83 (DNA-directed RNA polymerase III subunit rpc4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004050 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 7.0e-105
Identity = 197/269 (73.23%), Postives = 229/269 (85.13%), Query Frame = 0


Query: 1   ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIF 60
           +SVQVAFGPGA S SSSIRTYGVPKV N SRKN+IEPE+ +DEE +LP+ +DV EDG  F
Sbjct: 67  SSVQVAFGPGAESTSSSIRTYGVPKVENGSRKNDIEPEVDEDEEFVLPVAMDVNEDGKFF 126

Query: 61  DQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEF 120
           D+K++DG  ES   +++ ++TK K+DY+EPWDY +SYYP TLPLR PYSG PE LD+AEF
Sbjct: 127 DKKTKDGIAES---SSSAMVTKTKRDYKEPWDYQNSYYPTTLPLRMPYSGDPERLDEAEF 186

Query: 121 GLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 180
           G DA +REYDEN+ IPALDL L +E  E T YFFQLPA LPLPKQSSTAT KEKVG+SRS
Sbjct: 187 GQDAMNREYDENSVIPALDLGLQDENTENTKYFFQLPARLPLPKQSSTATGKEKVGNSRS 246

Query: 181 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 240
           SNS  +S+L DLKKL  GCMGKLLIYKSGA+KLRLG+ LYDVSSGS+CSFLQHVVAINTE
Sbjct: 247 SNSTSSSDLDDLKKLSAGCMGKLLIYKSGAIKLRLGDILYDVSSGSDCSFLQHVVAINTE 306

Query: 241 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           +G CCDLGD+GKRVVVTPDISSLLN+VT+
Sbjct: 307 KGQCCDLGDIGKRVVVTPDISSLLNSVTN 332

[Close]

BLAST of MS000289 vs. ExPASy TrEMBL
Match: A0A0A0KZ61 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022870 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.6e-104
Identity = 198/269 (73.61%), Postives = 226/269 (84.01%), Query Frame = 0


Query: 1   ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIF 60
           +SVQVAFGPGA S SSSIRTYGVPKV N SRKN+IEPE+ +DEE +LP+  DV EDG  F
Sbjct: 67  SSVQVAFGPGAESTSSSIRTYGVPKVENGSRKNDIEPEVDEDEEFVLPVARDVNEDGKYF 126

Query: 61  DQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEF 120
           D+K++DG  ES   +++ + TK K+DY+EPWDY +SYYP TLPLR PYSG PE LD+AEF
Sbjct: 127 DKKTKDGITES---SSSAMETKTKRDYKEPWDYQNSYYPTTLPLRMPYSGDPERLDEAEF 186

Query: 121 GLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 180
           G D  +REYDEN+ IPALDL LL+E  E T YFFQLPA LPLPKQSSTAT KEKVG+SRS
Sbjct: 187 GQDVMNREYDENSVIPALDLGLLDENTESTKYFFQLPARLPLPKQSSTATGKEKVGNSRS 246

Query: 181 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 240
           SNS  +S+L DLKKL  GCMGKLLIYKSGA+KLRLG+ LYDVSSGSNCSFLQHVVAINTE
Sbjct: 247 SNSTSSSDLDDLKKLSAGCMGKLLIYKSGAIKLRLGDILYDVSSGSNCSFLQHVVAINTE 306

Query: 241 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           EG CCDLGD+G RVVVTPDISSLLN+VT+
Sbjct: 307 EGQCCDLGDIGNRVVVTPDISSLLNSVTN 332

[Close]

BLAST of MS000289 vs. ExPASy TrEMBL
Match: A0A1S3BYJ9 (DNA-directed RNA polymerase III subunit rpc4-like OS=Cucumis melo OX=3656 GN=LOC103494480 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.8e-104
Identity = 196/269 (72.86%), Postives = 228/269 (84.76%), Query Frame = 0


Query: 1   ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIF 60
           +SVQVAFGPGA S SSSIRTYGVPKV N SRKN+IEPE+ +DEE +LP+ +DV EDG  F
Sbjct: 67  SSVQVAFGPGAESTSSSIRTYGVPKVENGSRKNDIEPEVDEDEEFVLPVAMDVNEDGKFF 126

Query: 61  DQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEF 120
           D+K++DG  ES   +++ ++TK K+DY+EPWDY +SYYP TLPLR PYSG  E LD+AEF
Sbjct: 127 DKKTKDGIAES---SSSAMVTKTKRDYKEPWDYQNSYYPTTLPLRMPYSGDHERLDEAEF 186

Query: 121 GLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRS 180
           G DA +REYDEN+ IPALDL L +E  E T YFFQLPA LPLPKQSSTAT KEKVG+SRS
Sbjct: 187 GQDAMNREYDENSVIPALDLGLQDENTENTKYFFQLPARLPLPKQSSTATGKEKVGNSRS 246

Query: 181 SNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTE 240
           SNS  +S+L DLKKL  GCMGKLLIYKSGA+KLRLG+ LYDVSSGS+CSFLQHVVAINTE
Sbjct: 247 SNSTSSSDLDDLKKLSAGCMGKLLIYKSGAIKLRLGDILYDVSSGSDCSFLQHVVAINTE 306

Query: 241 EGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           +G CCDLGD+GKRVVVTPDISSLLN+VT+
Sbjct: 307 KGQCCDLGDIGKRVVVTPDISSLLNSVTN 332

[Close]

BLAST of MS000289 vs. TAIR 10
Match: AT4G25180.1 (RNA polymerase III RPC4 )

HSP 1 Score: 160.6 bits (405), Expect = 1.7e-39
Identity = 109/270 (40.37%), Postives = 147/270 (54.44%), Query Frame = 0


Query: 1   ASVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIF 60
           +S +VAF P  +    +IR++GVPK  +    +++ P       SILP    V       
Sbjct: 62  SSPEVAFQPSLSPL--AIRSFGVPK-EDDKPNSDVNP---SSPASILPAVSSV------- 121

Query: 61  DQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEF 120
               EDG            +T+   DY EPWDY +SYYP  LPLRKP SG  ELLD+ EF
Sbjct: 122 TAAQEDGEEVHNF------VTRTGDDYVEPWDYRNSYYPTVLPLRKPNSGDIELLDQEEF 181

Query: 121 GLDAPDREYDENAAIPALDLELLE-EKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSR 180
           G  A +R+YDEN    A +L L   +  +K M+ F++P CLP+ KQ++ AT K  V    
Sbjct: 182 GEVAKNRDYDENTINSAEELGLTSVQHSKKQMFIFKIPDCLPVVKQTTGATTKRSVREYS 241

Query: 181 SSNSKGTSELGDLKKLPGGCMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINT 240
           S  S         + LP G MGK+L+YKSGAVKL++G+ L+DVS G        VVAI+ 
Sbjct: 242 SGIS------NPFEGLPEGFMGKMLVYKSGAVKLKVGDALFDVSPGPGTKIPNDVVAIDI 301

Query: 241 EEGHCCDLGDVGKRVVVTPDISSLLNAVTD 270
           +  +C  +G   K V VTPD+ SLLN  +D
Sbjct: 302 KGRNCSRIGSSAKFVTVTPDVESLLNPASD 306

[Close]

BLAST of MS000289 vs. TAIR 10
Match: AT5G09380.1 (RNA polymerase III RPC4 )

HSP 1 Score: 149.8 bits (377), Expect = 3.1e-36
Identity = 88/184 (47.83%), Postives = 119/184 (64.67%), Query Frame = 0


Query: 86  DYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFGLDAPDREYDENAAIPALDLELLEE 145
           +Y+EPWDY +SYYP TLP+R+PY+G PE+LD  EF        + E++   A +L L+E+
Sbjct: 98  EYKEPWDY-YSYYPITLPMRRPYAGDPEVLDVEEF---MQAGGHHEDSLNTAANLGLMED 157

Query: 146 KEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSSNSKGTSE--LGDLKKLPGGCMGKL 205
             E+ M+F +LP+ +PL   +ST T   +       N KG  E    DLK LP G MGKL
Sbjct: 158 SGEQKMFFMRLPS-VPL---ASTPTENLET----RPNIKGPVEKKTVDLKALPEGYMGKL 217

Query: 206 LIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEEGHCCDLGDVGKRVVVTPDISSL 265
           L+YKSGAVK++LGE LYDVS G    F Q V+ +NTE+ +CC +GDV K  V+TPDI S+
Sbjct: 218 LVYKSGAVKMKLGEVLYDVSPGLKSEFAQDVMVVNTEQKNCCLVGDVYKHAVLTPDIDSI 269

Query: 266 LNAV 268
           L  +
Sbjct: 278 LKDI 269

The following BLAST results are available for this feature:

BLAST of MS000289 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141051.1	1.3e-145	99.63	DNA-directed RNA polymerase III subunit RPC4-like isoform X2 [Momordica charanti...	[more]
XP_022141050.1	3.2e-144	99.26	DNA-directed RNA polymerase III subunit RPC4-like isoform X1 [Momordica charanti...	[more]
XP_038879339.1	2.0e-109	75.84	DNA-directed RNA polymerase III subunit rpc4-like [Benincasa hispida]	[more]
KAA0044650.1	1.5e-104	73.23	DNA-directed RNA polymerase III subunit rpc4-like [Cucumis melo var. makuwa]	[more]
XP_004146914.1	3.2e-104	73.61	DNA-directed RNA polymerase III subunit rpc4 [Cucumis sativus] >KGN53151.1 hypot...	[more]

BLAST of MS000289 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05423	1.5e-08	31.95	DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D P...	[more]
Q91WD1	4.5e-08	26.59	DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d ...	[more]
Q5E9Z7	1.7e-07	33.79	DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=...	[more]
Q8T1V7	5.5e-06	37.66	DNA-directed RNA polymerase III subunit rpc4 OS=Dictyostelium discoideum OX=4468...	[more]
P25441	4.7e-05	29.01	DNA-directed RNA polymerase III subunit RPC4 OS=Saccharomyces cerevisiae (strain...	[more]

BLAST of MS000289 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHV0	6.3e-146	99.63	DNA-directed RNA polymerase III subunit RPC4-like isoform X2 OS=Momordica charan...	[more]
A0A6J1CGV5	1.5e-144	99.26	DNA-directed RNA polymerase III subunit RPC4-like isoform X1 OS=Momordica charan...	[more]
A0A5A7TN83	7.0e-105	73.23	DNA-directed RNA polymerase III subunit rpc4-like OS=Cucumis melo var. makuwa OX...	[more]
A0A0A0KZ61	1.6e-104	73.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022870 PE=4 SV=1	[more]
A0A1S3BYJ9	7.8e-104	72.86	DNA-directed RNA polymerase III subunit rpc4-like OS=Cucumis melo OX=3656 GN=LOC...	[more]

BLAST of MS000289 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25180.1	1.7e-39	40.37	RNA polymerase III RPC4	[more]
AT5G09380.1	3.1e-36	47.83	RNA polymerase III RPC4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PFAM	PF05132	RNA_pol_Rpc4	coord: 149..260 e-value: 9.3E-27 score: 94.3
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PANTHER	PTHR13408	DNA-DIRECTED RNA POLYMERASE III	coord: 2..269
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..45
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 165..189
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 9..34
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 165..188
None	No IPR available	PANTHER	PTHR13408:SF6	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4-LIKE ISOFORM X1	coord: 2..269

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000289.1	MS000289.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding