MS000286 (gene) Bitter gourd (TR) v1

Overview


Name	MS000286
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	gibberellin 20-oxidase-like protein
Location	scaffold44: 618220 .. 619732 (+)
RNA-Seq Expression	MS000286
Synteny	MS000286

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGAATCTCAAACCTGTGTGGAGCTTCCAGTGATCGACCTTTCTCAGCCTTTGAGCGAATCGATTCTCTGCTCTCTTTGTGCGGCCTGCAACGAATGGGGATTCTTCTACATCACCAACCATGGCGTCTCCGACGAATTTTACCAGAAACTGATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGACACCAAAATCAAAGTCGGAATTTCGTCGTACGTGCCGAGATTTACGGCGTCGCCTTTCTTTGAGAGCTTCAAGCTTTCGGGGCCGGACTTCTCTGCCTCTGCTCAACTCTTGACGGAAGCGCTCCTCGGCCATCACAATACTCAATTTAGGTAAGAAATAGATTACCAATAAGTTTTTTCCCTTTTTAGGAAGAATAAGTTTGCGTTTTTTTTTTCAATTCCAAAAAAAATTTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAATTTTAAAAATCTTTTTTTTTTAATATAACAATTTTAAAAATATTTTTTAATATTGACAAAAATAAAAAAATTTTATTTTAAAAAATATAATAGAAATTAATATATTTATCGAGCATACTTTATTCTGATTTGTTTAGGTCTCATAATGCATAGTGTCAACTTGAACTCGCGGCCAACTTGAATGTAACTTAACTAAGAGGTGACTAAAAAAATCGAAGAATCTTCAACTTAAAAATAAATAAATCATCAAATAAACAATCGAATCATTTTTTTTTCTTTTTTCCCCTTTCTACCAAGTTCATTTACATAATGTCCCTCTTCTTCGTTCATTTATTTATGAAAATAATCTCTCTTGTGAGTTGAATTTGTTTGCAGCAACCTACTAGAGGAATATGGAAGCAAAATGACTGACCTATCTAAAAGAATAGTCAAGCTATTACTAAAGGTCTTGGGAGATAATTTCGAAAACAAATTTTATGATTCTGAGTTCAAAAACTGTGATGGATATCTTAGGATAAACAACTACTCTCCTCGAGACTCCAATGAAGAAGTTGAAGGGCTTGGGAAACACACTGATATTAGCTGCGTGACAATTCTTTTTCAAGATAAACTTGGTGGACTTCAGATGAGATCTAAAGGAGGAGAGTGGATGGACATAAGGCCTTGTGAGAACGCTCTTGTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGCAATGAGAGGTTGAGATCAGCAGAGCATCGAGTTGTCTTGAGGCAAAATGTAAAGAGATTCTCTATGGCATTCTTTTGGTCATATGTAGATGATGAGGAGATATATGCCCCAAGCGAGGTTGTTGGAGAAGGGAATGTGAGGCTTTATCGTACATTTTCAACAAAAGAGTACAGGATGTTCAGGGCAACTGGTGCGAGAGGGACCTATGAGAAAGTTGGAGCCAATGTAAAAGAATTTGCTGGAATTGTAGGA

mRNA sequence

ATGTCGGAATCTCAAACCTGTGTGGAGCTTCCAGTGATCGACCTTTCTCAGCCTTTGAGCGAATCGATTCTCTGCTCTCTTTGTGCGGCCTGCAACGAATGGGGATTCTTCTACATCACCAACCATGGCGTCTCCGACGAATTTTACCAGAAACTGATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGACACCAAAATCAAAGTCGGAATTTCGTCGTACGTGCCGAGATTTACGGCGTCGCCTTTCTTTGAGAGCTTCAAGCTTTCGGGGCCGGACTTCTCTGCCTCTGCTCAACTCTTGACGGAAGCGCTCCTCGGCCATCACAATACTCAATTTAGCAACCTACTAGAGGAATATGGAAGCAAAATGACTGACCTATCTAAAAGAATAGTCAAGCTATTACTAAAGGTCTTGGGAGATAATTTCGAAAACAAATTTTATGATTCTGAGTTCAAAAACTGTGATGGATATCTTAGGATAAACAACTACTCTCCTCGAGACTCCAATGAAGAAGTTGAAGGGCTTGGGAAACACACTGATATTAGCTGCGTGACAATTCTTTTTCAAGATAAACTTGGTGGACTTCAGATGAGATCTAAAGGAGGAGAGTGGATGGACATAAGGCCTTGTGAGAACGCTCTTGTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGCAATGAGAGGTTGAGATCAGCAGAGCATCGAGTTGTCTTGAGGCAAAATGTAAAGAGATTCTCTATGGCATTCTTTTGGTCATATGTAGATGATGAGGAGATATATGCCCCAAGCGAGGTTGTTGGAGAAGGGAATGTGAGGCTTTATCGTACATTTTCAACAAAAGAGTACAGGATGTTCAGGGCAACTGGTGCGAGAGGGACCTATGAGAAAGTTGGAGCCAATGTAAAAGAATTTGCTGGAATTGTAGGA

Coding sequence (CDS)

ATGTCGGAATCTCAAACCTGTGTGGAGCTTCCAGTGATCGACCTTTCTCAGCCTTTGAGCGAATCGATTCTCTGCTCTCTTTGTGCGGCCTGCAACGAATGGGGATTCTTCTACATCACCAACCATGGCGTCTCCGACGAATTTTACCAGAAACTGATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGACACCAAAATCAAAGTCGGAATTTCGTCGTACGTGCCGAGATTTACGGCGTCGCCTTTCTTTGAGAGCTTCAAGCTTTCGGGGCCGGACTTCTCTGCCTCTGCTCAACTCTTGACGGAAGCGCTCCTCGGCCATCACAATACTCAATTTAGCAACCTACTAGAGGAATATGGAAGCAAAATGACTGACCTATCTAAAAGAATAGTCAAGCTATTACTAAAGGTCTTGGGAGATAATTTCGAAAACAAATTTTATGATTCTGAGTTCAAAAACTGTGATGGATATCTTAGGATAAACAACTACTCTCCTCGAGACTCCAATGAAGAAGTTGAAGGGCTTGGGAAACACACTGATATTAGCTGCGTGACAATTCTTTTTCAAGATAAACTTGGTGGACTTCAGATGAGATCTAAAGGAGGAGAGTGGATGGACATAAGGCCTTGTGAGAACGCTCTTGTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGCAATGAGAGGTTGAGATCAGCAGAGCATCGAGTTGTCTTGAGGCAAAATGTAAAGAGATTCTCTATGGCATTCTTTTGGTCATATGTAGATGATGAGGAGATATATGCCCCAAGCGAGGTTGTTGGAGAAGGGAATGTGAGGCTTTATCGTACATTTTCAACAAAAGAGTACAGGATGTTCAGGGCAACTGGTGCGAGAGGGACCTATGAGAAAGTTGGAGCCAATGTAAAAGAATTTGCTGGAATTGTAGGA

Protein sequence

MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSNEEVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKVGANVKEFAGIVG

Homology

[Close]

BLAST of MS000286 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 635.2 bits (1637), Expect = 3.0e-178
Identity = 310/312 (99.36%), Postives = 311/312 (99.68%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
           YGSKMT+LSKRIVKLLLKVLGDNFENKFYD EFKNCDGYLRINNYSPRDSNEEVEGLGKH
Sbjct: 121 YGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300

Query: 301 GANVKEFAGIVG 313
           GANVKEFAGIVG
Sbjct: 301 GANVKEFAGIVG 312

[Close]

BLAST of MS000286 vs. NCBI nr
Match: XP_022141253.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 534.6 bits (1376), Expect = 5.5e-148
Identity = 264/317 (83.28%), Postives = 280/317 (88.33%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+FSNLLEE
Sbjct: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSN-----EEVE 180
           YGSKM +LSKRIV+LLLK+L D FENKFYDSEF NC G L INNYSPRDSN     EE+E
Sbjct: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180

Query: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300

Query: 301 TYEKVGANVKEFAGIVG 313
            YEKVGA VK+FAGIVG
Sbjct: 301 NYEKVGATVKDFAGIVG 317

[Close]

BLAST of MS000286 vs. NCBI nr
Match: XP_022141255.1 (gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia])

HSP 1 Score: 524.6 bits (1350), Expect = 5.7e-145
Identity = 266/312 (85.26%), Postives = 266/312 (85.26%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF      
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF------ 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
                                                   RINNYSPRDSNEEVEGLGKH
Sbjct: 121 ----------------------------------------RINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 266

Query: 301 GANVKEFAGIVG 313
           GANVKEFAGIVG
Sbjct: 301 GANVKEFAGIVG 266

[Close]

BLAST of MS000286 vs. NCBI nr
Match: XP_022140867.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 503.1 bits (1294), Expect = 1.8e-138
Identity = 252/321 (78.50%), Postives = 278/321 (86.60%), Query Frame = 0


Query: 1   MSESQT-CVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADEL 60
           MSES++  VELPVID+ + LSES L SL  AC+EWGFFYI NHGVS EFYQKLISIAD+L
Sbjct: 1   MSESKSYTVELPVIDVYEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 60

Query: 61  FRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLE 120
           F LP +TK KVG S Y+PRF ASPF+ESFK SGPDFSASA  LTE LLG H+ +FSNLL+
Sbjct: 61  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLLGKHDAEFSNLLK 120

Query: 121 EYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSN-----EEV 180
           EYGSKMTD+S+RIVKLLLK+LGDNFENKFYDSEF NCDGYLR+NNYSP+DSN     EEV
Sbjct: 121 EYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIEEV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLGKHTDISCVTILFQD +GGLQMRSK GEWMDI+PCE+ALVVNIGDFM AWSNE+LRS
Sbjct: 181 EGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKLRS 240

Query: 241 AEHRVVLRQN-VKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT-- 300
           AEHRVVLRQN VKRFSM F WS+ DD+EIYAPSEVVGEGN RLY+ F TKEYR+FRAT  
Sbjct: 241 AEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRATPR 300

Query: 301 GARGTYEKVGANVKEFAGIVG 313
           GAR  YEKVGANV +FAGIVG
Sbjct: 301 GARENYEKVGANVTDFAGIVG 321

[Close]

BLAST of MS000286 vs. NCBI nr
Match: XP_031265650.1 (gibberellin 20-oxidase-like protein [Pistacia vera])

HSP 1 Score: 405.2 bits (1040), Expect = 5.1e-109
Identity = 196/316 (62.03%), Postives = 245/316 (77.53%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           M+ESQT VELPV+D+SQPLS S L +L AAC EWGFFYITNHGVS + Y+KL S+++ LF
Sbjct: 1   MAESQTTVELPVLDISQPLSTSKLSALSAACKEWGFFYITNHGVSKDLYRKLHSLSNHLF 60

Query: 61  RLPQDTKIKVG----ISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSN 120
            LP +TK+KVG    I +Y P F ASPFFESF++SGPDFS+SAQ   EALL   N+ FS 
Sbjct: 61  NLPYETKVKVGPFSSIKTYTPHFIASPFFESFRVSGPDFSSSAQSSVEALLKEPNSDFSE 120

Query: 121 LLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDS--NEEV 180
            L+EYG KMT+LSK IV+ +L  LG +F+ KFY+SEF  C GYLRI NYSP ++   ++V
Sbjct: 121 TLKEYGRKMTELSKAIVQFVLMSLGKDFQKKFYESEFSKCHGYLRIINYSPPENVVEKQV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLG HTD+SCVTI++QD++GGLQ+RSK G WMDI PCE+ LVVNIGD M AWSN RLRS
Sbjct: 181 EGLGMHTDMSCVTIVYQDEIGGLQVRSKEGTWMDINPCEDTLVVNIGDLMHAWSNGRLRS 240

Query: 241 AEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGAR 300
           +EHRVVL++N  RFS+AFFW + D++ I AP EVVGE + R+Y+TF  K+Y  FR +  +
Sbjct: 241 SEHRVVLKRNANRFSLAFFWCFEDEKNISAPDEVVGEEDNRVYKTFICKDYLRFRESSEK 300

Query: 301 GTYEKVGANVKEFAGI 311
           G +EKVG  VK+FAGI
Sbjct: 301 GKFEKVGFTVKQFAGI 316

[Close]

BLAST of MS000286 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.1e-85
Identity = 160/320 (50.00%), Postives = 223/320 (69.69%), Query Frame = 0


Query: 8   VELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTK 67
           +ELPV D+S+PLSES L SL  AC EWGFFY+TNHGVS + Y+KL   +  +F L  + K
Sbjct: 3   LELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 62

Query: 68  IKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGH-HNTQFSNLLEEYGSKMT 127
           +K+G S+Y PRF ASPFFES ++SGPDF ASA+   +A      + +FS L++EYG KMT
Sbjct: 63  MKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEYGEKMT 122

Query: 128 DLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYS-PRDSNEE---------VEG 187
            L ++I+K +L   GD+  +K+Y+SEF NC GY RINNY+ P D  ++         +EG
Sbjct: 123 KLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLIEG 182

Query: 188 LGKHTDISCVTILFQDKLGGLQMRSKGG-EWMDIRPCENALVVNIGDFMQAWSNERLRSA 247
           LG HTD+SC+TI+ QD +GGLQ+R++ G   MDI P + ALVVN+GD + AW+N RLRS+
Sbjct: 183 LGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLRSS 242

Query: 248 EHRVVLRQN---VKRFSMAFFWSYVDDEEIYAPSEVVG--EGNVRLYRTFSTKEYRMFRA 307
           +HRV+L++      RFS+AFFW + D + ++AP EVVG  EG +R++R+F   +Y  FR 
Sbjct: 243 QHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRFRE 302

Query: 308 TGARGTYEKVGANVKEFAGI 311
           +  +G +EKVG  V++FA I
Sbjct: 303 SNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of MS000286 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-35
Identity = 98/310 (31.61%), Postives = 160/310 (51.61%), Query Frame = 0


Query: 8   VELPVIDLSQPLS-ESILCSLC-----AACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           ++LPVIDLS   S E +    C     AA  EWGFF I NHG+  + ++ ++    +LF 
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQ---------LLTE-ALLGHHN 127
            P   K++   S             S++   P  ++ AQ         +L+E + +    
Sbjct: 98  QPFSVKVRERFSDLSK--------NSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDR 157

Query: 128 TQFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSN 187
                ++E Y  ++  +++ I ++L K +  N  ++++++ F+  + +LR+N Y P    
Sbjct: 158 NNLRTIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG 217

Query: 188 EEVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNER 247
            EV GL  HTD S +TIL QD++GGL++ +  G+W+ ++PC  AL VNIGD  QA SN  
Sbjct: 218 SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALTVNIGDMFQALSNGV 277

Query: 248 LRSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT 302
            +S  HRV+   N++R S+AFF         Y  +E+   G  + YR FS +EY+     
Sbjct: 278 YQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKYRRFSFREYKEQSEH 330

[Close]

BLAST of MS000286 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 104/320 (32.50%), Postives = 157/320 (49.06%), Query Frame = 0


Query: 8   VELPVIDLSQPLS------ESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           VELPVID+S+ +       E    ++  A  EWGFF + NHG+S +  +K+      +FR
Sbjct: 40  VELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFR 99

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQL---------LTEALLGHHNT 127
            P D K K        +F+A     S++   P  ++  QL         +T+       T
Sbjct: 100 EPFDKKSK------SEKFSAG----SYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFT 159

Query: 128 QFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSNE 187
             S+ +E++ S+   L+  + ++L +  G N  + F+         YLR+N Y P     
Sbjct: 160 TLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKPS 219

Query: 188 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 247
           EV GL  HTD   +TIL+QD++GGLQ+  K   W+ ++P   AL++NIGD  QAWSN   
Sbjct: 220 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKALIINIGDLFQAWSNGMY 279

Query: 248 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 307
           +S EHRV+    V+RFS A+F     D  I   S+         YR FS +E+R      
Sbjct: 280 KSVEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPA------YRNFSFREFR------ 329

Query: 308 ARGTYEKVGANVKEFAGIVG 313
                ++V  +VK+F   VG
Sbjct: 340 -----QQVQEDVKKFGFKVG 329

[Close]

BLAST of MS000286 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.6e-33
Identity = 95/291 (32.65%), Postives = 142/291 (48.80%), Query Frame = 0


Query: 10  LPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTKIK 69
           +PVIDLS P    +   +  A   WG F I NHG+S +    + S++  LF +P + K++
Sbjct: 49  IPVIDLSNP---DVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLE 108

Query: 70  V-----GISSY-VPRFTASPFFESFKLSGPDFSA---SAQLLTEALLGHHNTQFSNLLEE 129
                 G+S Y  PR   SPFFE  K+    F+    S +     L  H +T++  +++E
Sbjct: 109 AASSDKGVSGYGEPRI--SPFFEK-KMWSEGFTIADDSYRNHFNTLWPHDHTKYCGIIQE 168

Query: 130 YGSKMTDLSKRIVKLLLKVLGDNFE-----NKFYDSEFKNCDGYLRINNYSPRDSNEEVE 189
           Y  +M  L+ R++  +L  LG   E     +K   S  K   G +R+N+Y      E   
Sbjct: 169 YVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHYPVCPEPERAM 228

Query: 190 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 249
           GL  HTD + +TIL Q   GGLQ+  +   W+ + P    LVVNIGD     SN ++ S 
Sbjct: 229 GLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSV 288

Query: 250 EHRVVLRQNVKRFSMAFFW-SYVDDEEIYAPSEVVGEGNVRLYRTFSTKEY 286
            HR  +     R S+A+ W     D +I   S++ G     LYR+ + KEY
Sbjct: 289 VHRAKVNHTRSRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEY 333

[Close]

BLAST of MS000286 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.0e-32
Identity = 97/308 (31.49%), Postives = 150/308 (48.70%), Query Frame = 0


Query: 9   ELPVIDLSQPLSESILC--SLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDT 68
           ELPVIDL+  +    +C   +  A +EWGFF +TNHGV     ++L      +FR P   
Sbjct: 41  ELPVIDLAGLMEAEQVCRADMVRAASEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQE 100

Query: 69  KIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHH----------NTQFSN 128
           K+   +  + P        ES++   P      QL       +H          + +   
Sbjct: 101 KVTERLLGFSP--------ESYRWGTPTAKCLEQLSWSE--AYHIPMTTPRPSTSIRARA 160

Query: 129 LLEEYGSKMTDLSKRIVKLLLKVL-GDNFENKFYDSEFKNCDGYLRINNYSPRDSNEEVE 188
           ++EE    M +L++++ ++L++ L G         +  + C  +LR+N Y P        
Sbjct: 161 VIEEVSRAMYELAQKLAEILMRGLPGAGEGETMVTTREETC--FLRLNRYPPCAMAMGGF 220

Query: 189 GLGKHTDISCVTILF--QDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLR 248
           GL  HTD   +TI+   QD +GGLQ+  KGG W+ ++P  + L+VN+GD +QAWSN+  +
Sbjct: 221 GLCPHTDSDLLTIVHQQQDTVGGLQL-LKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYK 280

Query: 249 SAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGA 302
           S EHRV+    ++RFSMAFF        I   S  V + +   YR+F+  EYR       
Sbjct: 281 SVEHRVMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAH-YRSFTFGEYRKQIMEDV 334

[Close]

BLAST of MS000286 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 8.5e-179
Identity = 311/312 (99.68%), Postives = 311/312 (99.68%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
           YGSKMTDLSKRIVKLLLKVLGDNFENKFYD EFKNCDGYLRINNYSPRDSNEEVEGLGKH
Sbjct: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300

Query: 301 GANVKEFAGIVG 313
           GANVKEFAGIVG
Sbjct: 301 GANVKEFAGIVG 312

[Close]

BLAST of MS000286 vs. ExPASy TrEMBL
Match: A0A6J1CJY7 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011696 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.7e-148
Identity = 264/317 (83.28%), Postives = 280/317 (88.33%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+FSNLLEE
Sbjct: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSN-----EEVE 180
           YGSKM +LSKRIV+LLLK+L D FENKFYDSEF NC G L INNYSPRDSN     EE+E
Sbjct: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180

Query: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300

Query: 301 TYEKVGANVKEFAGIVG 313
            YEKVGA VK+FAGIVG
Sbjct: 301 NYEKVGATVKDFAGIVG 317

[Close]

BLAST of MS000286 vs. ExPASy TrEMBL
Match: A0A6J1CHI8 (gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.8e-145
Identity = 266/312 (85.26%), Postives = 266/312 (85.26%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF      
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF------ 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
                                                   RINNYSPRDSNEEVEGLGKH
Sbjct: 121 ----------------------------------------RINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 266

Query: 301 GANVKEFAGIVG 313
           GANVKEFAGIVG
Sbjct: 301 GANVKEFAGIVG 266

[Close]

BLAST of MS000286 vs. ExPASy TrEMBL
Match: A0A6J1CI97 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011430 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.6e-139
Identity = 252/321 (78.50%), Postives = 278/321 (86.60%), Query Frame = 0


Query: 1   MSESQT-CVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADEL 60
           MSES++  VELPVID+ + LSES L SL  AC+EWGFFYI NHGVS EFYQKLISIAD+L
Sbjct: 1   MSESKSYTVELPVIDVYEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 60

Query: 61  FRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLE 120
           F LP +TK KVG S Y+PRF ASPF+ESFK SGPDFSASA  LTE LLG H+ +FSNLL+
Sbjct: 61  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLLGKHDAEFSNLLK 120

Query: 121 EYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSN-----EEV 180
           EYGSKMTD+S+RIVKLLLK+LGDNFENKFYDSEF NCDGYLR+NNYSP+DSN     EEV
Sbjct: 121 EYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIEEV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLGKHTDISCVTILFQD +GGLQMRSK GEWMDI+PCE+ALVVNIGDFM AWSNE+LRS
Sbjct: 181 EGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKLRS 240

Query: 241 AEHRVVLRQN-VKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT-- 300
           AEHRVVLRQN VKRFSM F WS+ DD+EIYAPSEVVGEGN RLY+ F TKEYR+FRAT  
Sbjct: 241 AEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRATPR 300

Query: 301 GARGTYEKVGANVKEFAGIVG 313
           GAR  YEKVGANV +FAGIVG
Sbjct: 301 GARENYEKVGANVTDFAGIVG 321

[Close]

BLAST of MS000286 vs. ExPASy TrEMBL
Match: A0A5N6QMY6 (Fe2OG dioxygenase domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_003930 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 3.0e-107
Identity = 189/315 (60.00%), Postives = 241/315 (76.51%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQT VELP++D+SQPL  S L SL   C +WGFF+I NHG+S +FY+KL  ++  LF
Sbjct: 1   MSESQTSVELPILDISQPLDPSFLSSLSEGCKKWGFFHIINHGISIDFYRKLYLLSKGLF 60

Query: 61  RLPQDTKIKVG----ISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSN 120
            LP DTKIK+G    I +Y P F ASP+FES ++SGP+F ASAQ   + L    +++FS 
Sbjct: 61  SLPSDTKIKLGPFSSIKTYTPHFIASPYFESLRVSGPNFFASAQSSADVLFAQQSSEFSV 120

Query: 121 LLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDS-NEEVE 180
           +L+E GSKM +LSK I+K+LL  LGD FE K+Y+SEFKNC GYLRINNYSP +S  +EVE
Sbjct: 121 ILQECGSKMAELSKTILKILLLSLGDGFEEKYYESEFKNCHGYLRINNYSPPESLEDEVE 180

Query: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240
           GLG HTD+SCVTI+ QD++GGLQ+RSK G+WMDIRPCE  LVVNIGD +QAWSNE+ RS+
Sbjct: 181 GLGMHTDMSCVTIVCQDEIGGLQVRSKEGKWMDIRPCEGTLVVNIGDMLQAWSNEKFRSS 240

Query: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300
           EHRV+L+Q   RFS+AFFW + D++ I AP EVVGEGN++LY+ F   +Y  FR +  RG
Sbjct: 241 EHRVILKQPANRFSLAFFWCFEDEKMIMAPDEVVGEGNMKLYKPFVCLDYLKFRESNERG 300

Query: 301 TYEKVGANVKEFAGI 311
            +EKVG  V++FAGI
Sbjct: 301 KFEKVGFTVRDFAGI 315

[Close]

BLAST of MS000286 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 2.5e-98
Identity = 167/316 (52.85%), Postives = 227/316 (71.84%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSE  + ++LPV+DL+QP+  SIL SL  AC EWGFFY+TNHG+S E + K+ S++ ++F
Sbjct: 1   MSELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALL-GHHNTQFSNLLE 120
           + P ++K+K+G  SY PR+ ASP+FES  +SGPDFS SA+   + L   HH  +    ++
Sbjct: 61  KAPLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELRETMQ 120

Query: 121 EYGSKMTDLSKRIVKLLLKV-LGDNFENKFYDSEFKNCDGYLRINNYSP----RDSNEEV 180
           EYG+KM +LSKR++K+LL + LGD    + Y ++F NC GYLR+ NY+P        E V
Sbjct: 121 EYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLG HTD+SC+TI++QD +GGLQMRSK G+W+DI PC + LVVNIGD MQAWSN RLRS
Sbjct: 181 EGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRLRS 240

Query: 241 AEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGAR 300
           +EHRVVLR+ V R S+AFF  + D++ I AP E+VGEG  R Y++F   EY  FR +   
Sbjct: 241 SEHRVVLRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEE 300

Query: 301 GTYEKVGANVKEFAGI 311
           G +EK+G  VK+FAG+
Sbjct: 301 GKFEKIGFTVKDFAGL 316

[Close]

BLAST of MS000286 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 316.6 bits (810), Expect = 2.2e-86
Identity = 160/320 (50.00%), Postives = 223/320 (69.69%), Query Frame = 0


Query: 8   VELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTK 67
           +ELPV D+S+PLSES L SL  AC EWGFFY+TNHGVS + Y+KL   +  +F L  + K
Sbjct: 3   LELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 62

Query: 68  IKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGH-HNTQFSNLLEEYGSKMT 127
           +K+G S+Y PRF ASPFFES ++SGPDF ASA+   +A      + +FS L++EYG KMT
Sbjct: 63  MKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEYGEKMT 122

Query: 128 DLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYS-PRDSNEE---------VEG 187
            L ++I+K +L   GD+  +K+Y+SEF NC GY RINNY+ P D  ++         +EG
Sbjct: 123 KLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLIEG 182

Query: 188 LGKHTDISCVTILFQDKLGGLQMRSKGG-EWMDIRPCENALVVNIGDFMQAWSNERLRSA 247
           LG HTD+SC+TI+ QD +GGLQ+R++ G   MDI P + ALVVN+GD + AW+N RLRS+
Sbjct: 183 LGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLRSS 242

Query: 248 EHRVVLRQN---VKRFSMAFFWSYVDDEEIYAPSEVVG--EGNVRLYRTFSTKEYRMFRA 307
           +HRV+L++      RFS+AFFW + D + ++AP EVVG  EG +R++R+F   +Y  FR 
Sbjct: 243 QHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRFRE 302

Query: 308 TGARGTYEKVGANVKEFAGI 311
           +  +G +EKVG  V++FA I
Sbjct: 303 SNEKGKFEKVGDTVEDFARI 321

[Close]

BLAST of MS000286 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 8.4e-62
Identity = 110/198 (55.56%), Postives = 145/198 (73.23%), Query Frame = 0


Query: 118 LEEYGSKMTDLSKRIVKLLLKV-LGDNFENKFYDSEFKNCDGYLRINNYSP----RDSNE 177
           ++EYG+KM +LSKR++K+LL + LGD    + Y ++F NC GYLR+ NY+P        E
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 178 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 237
            VEGLG HTD+SC+TI++QD +GGLQMRSK G+W+DI PC + LVVNIGD MQAWSN RL
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRL 120

Query: 238 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 297
           RS+EHRVVLR+ V R S+AFF  + D++ I AP E+VGEG  R Y++F   EY  FR + 
Sbjct: 121 RSSEHRVVLRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSN 180

Query: 298 ARGTYEKVGANVKEFAGI 311
             G +EK+G  VK+FAG+
Sbjct: 181 EEGKFEKIGFTVKDFAGL 198

[Close]

BLAST of MS000286 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 151.8 bits (382), Expect = 9.4e-37
Identity = 98/310 (31.61%), Postives = 160/310 (51.61%), Query Frame = 0


Query: 8   VELPVIDLSQPLS-ESILCSLC-----AACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           ++LPVIDLS   S E +    C     AA  EWGFF I NHG+  + ++ ++    +LF 
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQ---------LLTE-ALLGHHN 127
            P   K++   S             S++   P  ++ AQ         +L+E + +    
Sbjct: 98  QPFSVKVRERFSDLSK--------NSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDR 157

Query: 128 TQFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSN 187
                ++E Y  ++  +++ I ++L K +  N  ++++++ F+  + +LR+N Y P    
Sbjct: 158 NNLRTIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG 217

Query: 188 EEVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNER 247
            EV GL  HTD S +TIL QD++GGL++ +  G+W+ ++PC  AL VNIGD  QA SN  
Sbjct: 218 SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALTVNIGDMFQALSNGV 277

Query: 248 LRSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT 302
            +S  HRV+   N++R S+AFF         Y  +E+   G  + YR FS +EY+     
Sbjct: 278 YQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKYRRFSFREYKEQSEH 330

[Close]

BLAST of MS000286 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 148.3 bits (373), Expect = 1.0e-35
Identity = 104/320 (32.50%), Postives = 157/320 (49.06%), Query Frame = 0


Query: 8   VELPVIDLSQPLS------ESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           VELPVID+S+ +       E    ++  A  EWGFF + NHG+S +  +K+      +FR
Sbjct: 40  VELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFR 99

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQL---------LTEALLGHHNT 127
            P D K K        +F+A     S++   P  ++  QL         +T+       T
Sbjct: 100 EPFDKKSK------SEKFSAG----SYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFT 159

Query: 128 QFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDSEFKNCDGYLRINNYSPRDSNE 187
             S+ +E++ S+   L+  + ++L +  G N  + F+         YLR+N Y P     
Sbjct: 160 TLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKPS 219

Query: 188 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 247
           EV GL  HTD   +TIL+QD++GGLQ+  K   W+ ++P   AL++NIGD  QAWSN   
Sbjct: 220 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKALIINIGDLFQAWSNGMY 279

Query: 248 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 307
           +S EHRV+    V+RFS A+F     D  I   S+         YR FS +E+R      
Sbjct: 280 KSVEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPA------YRNFSFREFR------ 329

Query: 308 ARGTYEKVGANVKEFAGIVG 313
                ++V  +VK+F   VG
Sbjct: 340 -----QQVQEDVKKFGFKVG 329

The following BLAST results are available for this feature:

BLAST of MS000286 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141254.1	3.0e-178	99.36	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]
XP_022141253.1	5.5e-148	83.28	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_022141255.1	5.7e-145	85.26	gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia]	[more]
XP_022140867.1	1.8e-138	78.50	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_031265650.1	5.1e-109	62.03	gibberellin 20-oxidase-like protein [Pistacia vera]	[more]

BLAST of MS000286 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	3.1e-85	50.00	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q9C6I4	1.3e-35	31.61	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
O49561	1.5e-34	32.50	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C971	3.6e-33	32.65	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q8LGZ9	4.0e-32	31.49	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of MS000286 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CI26	8.5e-179	99.68	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1CJY7	2.7e-148	83.28	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110116...	[more]
A0A6J1CHI8	2.8e-145	85.26	gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1CI97	8.6e-139	78.50	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110114...	[more]
A0A5N6QMY6	3.0e-107	60.00	Fe2OG dioxygenase domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH...	[more]

BLAST of MS000286 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	2.5e-98	52.85	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	2.2e-86	50.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	8.4e-62	55.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G50960.1	9.4e-37	31.61	gibberellin 2-oxidase 7	[more]
AT4G21200.1	1.0e-35	32.50	gibberellin 2-oxidase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 216..242 score: 26.94 coord: 27..44 score: 53.54 coord: 180..201 score: 37.6
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 2..305
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 2..305
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 6..292
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 161..253 e-value: 1.7E-22 score: 79.8
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..288 e-value: 2.3E-73 score: 249.6
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..74 e-value: 2.1E-16 score: 60.7
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 155..256 score: 10.948321

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000286.1	MS000286.1	mRNA