MS000276 (gene) Bitter gourd (TR) v1

BLAST of MS000276 vs. NCBI nr
Match: XP_022141172.1 (probable inactive serine/threonine-protein kinase fnkC [Momordica charantia])

HSP 1 Score: 607.4 bits (1565), Expect = 6.4e-170
Identity = 293/297 (98.65%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 60
           IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL
Sbjct: 22  IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 81

Query: 61  YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN 120
           YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN
Sbjct: 82  YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN 141

Query: 121 ALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT 180
           ALNEASNGFVVDDSCTFGVE SVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT
Sbjct: 142 ALNEASNGFVVDDSCTFGVELSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT 201

Query: 181 SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTK 240
           SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESE IPIGKKIYAKYFLGIYNFGTK
Sbjct: 202 SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESENIPIGKKIYAKYFLGIYNFGTK 261

Query: 241 KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSY+QTDGLFL+ARIVAMSTVEEF
Sbjct: 262 KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYVQTDGLFLKARIVAMSTVEEF 318

BLAST of MS000276 vs. NCBI nr
Match: XP_008453868.1 (PREDICTED: uncharacterized protein LOC103494460 isoform X1 [Cucumis melo] >KAA0044674.1 ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 [Cucumis melo var. makuwa] >TYK16908.1 ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 430.3 bits (1105), Expect = 1.4e-116
Identity = 198/298 (66.44%), Postives = 248/298 (83.22%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 60
           I+S R++PP+HY F+I+SYSVLS+I M+KCESGDFEVDGY WKLIL+PNG+ +VEDHISL
Sbjct: 21  IKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGYMWKLILYPNGNGEVEDHISL 80

Query: 61  YLAISSK-GNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSH 120
           +LAIS+   N LPLGWEVRVIFRF IFDQIRDNYLTIQDGK+RKYSKMK+EHG THL+SH
Sbjct: 81  FLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISH 140

Query: 121 NALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESY 180
           N  ++ASNGF+V++SCTFGVE S+LK SNKGE L+I +EPQ D + W +Y FS+ T +SY
Sbjct: 141 NVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREPQQDTYFWTLYSFSAKTNQSY 200

Query: 181 TSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGT 240
            S+PF VKGRKWRM +YPNG+S+GK +++S+YLKLD SETIP+GKKIYAK+ LG+YNF  
Sbjct: 201 ISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSETIPLGKKIYAKFLLGVYNFSA 260

Query: 241 KKYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           KKY +KS +HWF +  +G GF EFL+ +++   S    Q D  +L+ARIVAMSTV+EF
Sbjct: 261 KKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHS----QNDAFYLKARIVAMSTVDEF 314

BLAST of MS000276 vs. NCBI nr
Match: XP_011653084.1 (MATH domain and coiled-coil domain-containing protein At3g58370 [Cucumis sativus] >KGN53168.1 hypothetical protein Csa_015064 [Cucumis sativus])

HSP 1 Score: 429.5 bits (1103), Expect = 2.4e-116
Identity = 195/297 (65.66%), Postives = 247/297 (83.16%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 60
           I+S R++PP+HY F+I+SYSVLS+I M+KCESGDFEVDGYKWKLIL+PNG+++VEDHISL
Sbjct: 22  IKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNGNEEVEDHISL 81

Query: 61  YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN 120
           +LA+S+  N LPLGWE+RVIFRF IFDQIRDNYLTIQDGK+RKYSKMK+EHG THL+SHN
Sbjct: 82  FLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHN 141

Query: 121 ALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT 180
             N+AS+GF+V + CTFGVE S+LK SNKGE L+I+KEPQ D + W +Y FS+     Y 
Sbjct: 142 VFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQQDTYFWTLYSFSALKQPFYI 201

Query: 181 SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTK 240
           S+PF VKGRKWRM +YP+G+S+GK +++S+YLKLD SETIP+GKKIYAK+ LG+YNF  K
Sbjct: 202 SEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYNFSAK 261

Query: 241 KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           KY +KS +HW+ +  +G+GF EFL+ K++   S    Q D  +L+ARIVAMSTVEEF
Sbjct: 262 KYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHS----QNDAFYLKARIVAMSTVEEF 314

BLAST of MS000276 vs. NCBI nr
Match: KAG6600198.1 (Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 429.1 bits (1102), Expect = 3.1e-116
Identity = 199/296 (67.23%), Postives = 246/296 (83.11%), Query Frame = 0

Query: 2   RSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISLY 61
           R+ R+EPP +YTF+INSYS LS+IGM+KCESG+F VDG+ WKLIL+PNGDD+VEDHISLY
Sbjct: 108 RTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFNWKLILYPNGDDEVEDHISLY 167

Query: 62  LAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHNA 121
           LAI+SK N LPLGWEV+VIFRF ++D+I+DNYLT+QDGK+RKYSKMK+EHG T  L+HNA
Sbjct: 168 LAITSKDN-LPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRKYSKMKSEHGFTKFLAHNA 227

Query: 122 LNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYTS 181
            N+ASNGF+VD+ CTFGVE SVLK SNKGESL+I KEPQL  F W I  FS+ T E Y S
Sbjct: 228 FNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLGSFFWSISSFSNKTNEFYLS 287

Query: 182 QPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTKK 241
           +PFTVKGRKWR+ +YPNG S GK +++S+YLKLD SET+P GKKIYAK+FL +YNFG KK
Sbjct: 288 EPFTVKGRKWRLTIYPNGLSSGKMSHISLYLKLDSSETVPTGKKIYAKFFLSVYNFGAKK 347

Query: 242 YFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           Y ++S++HWF ++TYG GF EFL+  DL+     +LQ D  +L+ARI AMS VE+F
Sbjct: 348 YVDQSNEHWFDNTTYGYGFHEFLSRNDLQ---PHHLQNDVFYLKARIAAMSVVEDF 399

BLAST of MS000276 vs. NCBI nr
Match: XP_038902857.1 (MATH domain and coiled-coil domain-containing protein At3g58400-like [Benincasa hispida])

HSP 1 Score: 428.7 bits (1101), Expect = 4.1e-116
Identity = 205/296 (69.26%), Postives = 245/296 (82.77%), Query Frame = 0

Query: 2   RSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISLY 61
           RS R++PP+HY FKI+SYSV S+I M+KCESGDFEVDGYKWKLIL+PNGD++V+DHISLY
Sbjct: 22  RSRRVDPPSHYAFKIDSYSVFSEIEMKKCESGDFEVDGYKWKLILYPNGDEEVQDHISLY 81

Query: 62  LAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHNA 121
           LAIS+K N LPLGWEVRVIFRF IFDQIRDNYLTIQDGK+R+YSKMK+EHG T LLSHN 
Sbjct: 82  LAISAKDN-LPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRRYSKMKSEHGFTQLLSHNI 141

Query: 122 LNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYTS 181
            N+ASNGF+VD+SCTFGVE SVLK SNKGE L+I KEPQ D F W IY FS    E Y S
Sbjct: 142 FNKASNGFLVDNSCTFGVEISVLKASNKGEILTIYKEPQQDTFFWSIYSFSVKINEFYIS 201

Query: 182 QPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTKK 241
           +PF VKGR+WRM +YPNG S GK +++S+YL+LD SETIP+GKKIYAK+ LG+YNF  KK
Sbjct: 202 EPFNVKGRQWRMVIYPNGHSRGKTSHISLYLRLDSSETIPLGKKIYAKFLLGVYNFVAKK 261

Query: 242 YFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           Y +KS + WF +++ G+GF EFL+ KD+      +LQ D L+L+ARIVAMS VEEF
Sbjct: 262 YIDKSKEVWFAATSTGNGFHEFLSRKDM----SPHLQNDVLYLKARIVAMSAVEEF 312

BLAST of MS000276 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 4.4e-12
Identity = 41/140 (29.29%), Postives = 69/140 (49.29%), Query Frame = 0

Query: 157 KEPQLDHFIWYIYPFSSYTGESYTSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDE 216
           ++P    F W I  FS      + S  F V G KWR+ ++P G++V    +LS+YL + +
Sbjct: 50  EDPPTLKFTWTIPNFSRQNTRKHYSDVFVVGGYKWRILIFPKGNNV---DHLSMYLDVSD 109

Query: 217 SETIPIGKKIYAKYFLGIYN-FGTKKYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKS 276
           + ++P G   YA++ L + N   T+    K   H F +     GF  F+ L +L D S+ 
Sbjct: 110 AASLPYGWSRYAQFSLAVVNQIHTRYTVRKETQHQFNARESDWGFTSFMPLSELYDPSRG 169

Query: 277 YLQTDGLFLRARIVAMSTVE 296
           YL  D + + A +     ++
Sbjct: 170 YLVNDTVLVEAEVAVRKVLD 186

BLAST of MS000276 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.4e-10
Identity = 37/140 (26.43%), Postives = 68/140 (48.57%), Query Frame = 0

Query: 157 KEPQLDHFIWYIYPFSSYTGESYTSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDE 216
           ++P    F W I  F+      + S  F V G KWR+ ++P G++V    +LS+YL + +
Sbjct: 49  EDPPSLKFTWTIPMFTRLNTRKHYSDVFVVGGYKWRILIFPKGNNV---DHLSMYLDVAD 108

Query: 217 SETIPIGKKIYAKYFLGIYNFGTKKY-FEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKS 276
           +  +P G   Y+++ L + N    +Y   K   H F +     GF  F+ L +L + ++ 
Sbjct: 109 AANLPYGWSRYSQFSLAVVNQVNNRYSIRKETQHQFNARESDWGFTSFMPLSELYEPTRG 168

Query: 277 YLQTDGLFLRARIVAMSTVE 296
           YL  D + + A +     ++
Sbjct: 169 YLVNDTVLIEAEVAVRKVLD 185

BLAST of MS000276 vs. ExPASy Swiss-Prot
Match: Q9M2J1 (MATH domain and coiled-coil domain-containing protein At3g58250 OS=Arabidopsis thaliana OX=3702 GN=At3g58250 PE=4 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 3.1e-10
Identity = 39/132 (29.55%), Postives = 64/132 (48.48%), Query Frame = 0

Query: 164 FIWYIYPFSSYTGESYTSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIG 223
           F W I  FSS   E   S  F + G +WR+  +P G+   K+ +LS+YL + ESE++P G
Sbjct: 11  FSWVIKNFSSLQSEKIYSDQFVIDGCRWRLLAFPKGNDT-KSDHLSLYLDVAESESLPCG 70

Query: 224 KKIYAKYFLGIYNFGTKKYFEKSDD-HWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGL 283
            + +A++   I N   +K  ++ +  HWF       GF   + L +LK     +L    L
Sbjct: 71  WRRHAQFSFTIVNHIPEKCSQRKETIHWFCEKVSDWGFTNLVPLIELKAEDSGFLVKGEL 130

Query: 284 FLRARIVAMSTV 295
            +   I  +  +
Sbjct: 131 KIVVEIEVLEVI 141

BLAST of MS000276 vs. ExPASy Swiss-Prot
Match: Q9M2I9 (MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis thaliana OX=3702 GN=At3g58270 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 9.1e-10
Identity = 43/143 (30.07%), Postives = 70/143 (48.95%), Query Frame = 0

Query: 7   EPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISLYLAISS 66
           E    +T+ I ++S        K  S +F VDG KW+L+  P G+    + +SLYLA++ 
Sbjct: 4   EVDNKFTWVIKNFSSQQS---RKNYSDEFFVDGCKWRLLAFPKGNG--VEKLSLYLAVAG 63

Query: 67  KGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHNALNEAS 126
               LP GW     F F + +Q+ D     ++ K   +    ++ G T +LS   L++  
Sbjct: 64  -SEFLPDGWRRHAYFHFSVVNQLSDELSQARETK-NWFDASTSDWGFTSMLSLKKLHDKD 123

Query: 127 NGFVVDDSCTFGVEFSVLKVSNK 150
            GF+V+      V+ SVL+V  K
Sbjct: 124 GGFLVNGELKIVVDVSVLEVIGK 139

BLAST of MS000276 vs. ExPASy Swiss-Prot
Match: Q9C870 (MATH domain and coiled-coil domain-containing protein At1g31390 OS=Arabidopsis thaliana OX=3702 GN=At1g31390 PE=4 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 4.2e-07
Identity = 38/115 (33.04%), Postives = 59/115 (51.30%), Query Frame = 0

Query: 166 WYIYPFSSYTGESYTSQPFTVKGRKWRMRMYPNGSSVGKATN--LSIYLKLDESETIPIG 225
           W I  FS    ++  S  F V   KW +  YP G+  G++TN  LS+YL + + +++P G
Sbjct: 11  WTIKNFSFVQSQAIDSDIFVVGDSKWHLVAYPKGN--GESTNKCLSLYLNVADFQSLPNG 70

Query: 226 KKIYAKYFLGIYNFGTKKYFEKS-DDHWFTSSTYGSGFPEFLALKDLKDSSKSYL 278
            K + KY L + N  ++K  E+     WF  + + SGF   L L  L D +  +L
Sbjct: 71  WKRHIKYRLTVVNQMSEKLSEQEVIQGWFYKNFHISGFQTMLPLSKLLDKNGGFL 123

BLAST of MS000276 vs. ExPASy TrEMBL
Match: A0A6J1CJ46 (probable inactive serine/threonine-protein kinase fnkC OS=Momordica charantia OX=3673 GN=LOC111011628 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.1e-170
Identity = 293/297 (98.65%), Postives = 295/297 (99.33%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 60
           IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL
Sbjct: 22  IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 81

Query: 61  YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN 120
           YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN
Sbjct: 82  YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN 141

Query: 121 ALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT 180
           ALNEASNGFVVDDSCTFGVE SVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT
Sbjct: 142 ALNEASNGFVVDDSCTFGVELSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT 201

Query: 181 SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTK 240
           SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESE IPIGKKIYAKYFLGIYNFGTK
Sbjct: 202 SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESENIPIGKKIYAKYFLGIYNFGTK 261

Query: 241 KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSY+QTDGLFL+ARIVAMSTVEEF
Sbjct: 262 KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYVQTDGLFLKARIVAMSTVEEF 318

BLAST of MS000276 vs. ExPASy TrEMBL
Match: A0A5A7TNH7 (Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00430 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 6.8e-117
Identity = 198/298 (66.44%), Postives = 248/298 (83.22%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 60
           I+S R++PP+HY F+I+SYSVLS+I M+KCESGDFEVDGY WKLIL+PNG+ +VEDHISL
Sbjct: 21  IKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGYMWKLILYPNGNGEVEDHISL 80

Query: 61  YLAISSK-GNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSH 120
           +LAIS+   N LPLGWEVRVIFRF IFDQIRDNYLTIQDGK+RKYSKMK+EHG THL+SH
Sbjct: 81  FLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISH 140

Query: 121 NALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESY 180
           N  ++ASNGF+V++SCTFGVE S+LK SNKGE L+I +EPQ D + W +Y FS+ T +SY
Sbjct: 141 NVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREPQQDTYFWTLYSFSAKTNQSY 200

Query: 181 TSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGT 240
            S+PF VKGRKWRM +YPNG+S+GK +++S+YLKLD SETIP+GKKIYAK+ LG+YNF  
Sbjct: 201 ISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSETIPLGKKIYAKFLLGVYNFSA 260

Query: 241 KKYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           KKY +KS +HWF +  +G GF EFL+ +++   S    Q D  +L+ARIVAMSTV+EF
Sbjct: 261 KKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHS----QNDAFYLKARIVAMSTVDEF 314

BLAST of MS000276 vs. ExPASy TrEMBL
Match: A0A1S3BYG6 (uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494460 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 6.8e-117
Identity = 198/298 (66.44%), Postives = 248/298 (83.22%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 60
           I+S R++PP+HY F+I+SYSVLS+I M+KCESGDFEVDGY WKLIL+PNG+ +VEDHISL
Sbjct: 21  IKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGYMWKLILYPNGNGEVEDHISL 80

Query: 61  YLAISSK-GNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSH 120
           +LAIS+   N LPLGWEVRVIFRF IFDQIRDNYLTIQDGK+RKYSKMK+EHG THL+SH
Sbjct: 81  FLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISH 140

Query: 121 NALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESY 180
           N  ++ASNGF+V++SCTFGVE S+LK SNKGE L+I +EPQ D + W +Y FS+ T +SY
Sbjct: 141 NVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREPQQDTYFWTLYSFSAKTNQSY 200

Query: 181 TSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGT 240
            S+PF VKGRKWRM +YPNG+S+GK +++S+YLKLD SETIP+GKKIYAK+ LG+YNF  
Sbjct: 201 ISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSETIPLGKKIYAKFLLGVYNFSA 260

Query: 241 KKYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           KKY +KS +HWF +  +G GF EFL+ +++   S    Q D  +L+ARIVAMSTV+EF
Sbjct: 261 KKYIDKSYEHWFKTPGHGDGFDEFLSRREISTHS----QNDAFYLKARIVAMSTVDEF 314

BLAST of MS000276 vs. ExPASy TrEMBL
Match: A0A0A0KUE1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 1.2e-116
Identity = 195/297 (65.66%), Postives = 247/297 (83.16%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISL 60
           I+S R++PP+HY F+I+SYSVLS+I M+KCESGDFEVDGYKWKLIL+PNG+++VEDHISL
Sbjct: 22  IKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGYKWKLILYPNGNEEVEDHISL 81

Query: 61  YLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHN 120
           +LA+S+  N LPLGWE+RVIFRF IFDQIRDNYLTIQDGK+RKYSKMK+EHG THL+SHN
Sbjct: 82  FLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGKMRKYSKMKSEHGFTHLISHN 141

Query: 121 ALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYT 180
             N+AS+GF+V + CTFGVE S+LK SNKGE L+I+KEPQ D + W +Y FS+     Y 
Sbjct: 142 VFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQQDTYFWTLYSFSALKQPFYI 201

Query: 181 SQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTK 240
           S+PF VKGRKWRM +YP+G+S+GK +++S+YLKLD SETIP+GKKIYAK+ LG+YNF  K
Sbjct: 202 SEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSETIPLGKKIYAKFILGVYNFSAK 261

Query: 241 KYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           KY +KS +HW+ +  +G+GF EFL+ K++   S    Q D  +L+ARIVAMSTVEEF
Sbjct: 262 KYIDKSYEHWYKTPGHGNGFDEFLSRKEISTHS----QNDAFYLKARIVAMSTVEEF 314

BLAST of MS000276 vs. ExPASy TrEMBL
Match: A0A6J1FUL4 (speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.6e-116
Identity = 198/296 (66.89%), Postives = 246/296 (83.11%), Query Frame = 0

Query: 2   RSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDKVEDHISLY 61
           R+ R EPP +YTF+INSYS LS+IGM+KCESG+F VDG+ WKLIL+PNGDD+VEDHISLY
Sbjct: 21  RTRRAEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFNWKLILYPNGDDEVEDHISLY 80

Query: 62  LAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHNA 121
           LAI+SK N LPLGWEV+VIFRF ++D+I+DNYLT+QDGK+RKYSKMK+EHG T  L+HNA
Sbjct: 81  LAITSKDN-LPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRKYSKMKSEHGFTKFLAHNA 140

Query: 122 LNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYTS 181
            N+ASNGF+VD+ CTFGVE SVLK SNKGESL+I KEPQL  F W I  FS+ T E Y S
Sbjct: 141 FNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEPQLGSFFWSISSFSNKTNEFYLS 200

Query: 182 QPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTKK 241
           +PFTVKGRKWR+ +YPNG S GK +++S+YLKLD SET+P GKKIYAK+FL +Y+FG K+
Sbjct: 201 EPFTVKGRKWRLMIYPNGLSSGKMSHISLYLKLDSSETVPTGKKIYAKFFLSVYDFGAKR 260

Query: 242 YFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVEEF 298
           Y ++S++HWF ++TYG GF EFL+  DL+     +LQ DG +L+ARI AMS VE+F
Sbjct: 261 YVDQSNEHWFDNTTYGYGFHEFLSRNDLR---PHHLQNDGFYLKARIAAMSVVEDF 312

BLAST of MS000276 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.0e-52
Identity = 105/292 (35.96%), Postives = 166/292 (56.85%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGD--DKVEDHI 60
           I+S    PPTHY  KI S+S+L+K  +E+ E+  FE  GYKWKL+L+PNG+     +DH+
Sbjct: 11  IKSISDAPPTHYMVKIESFSLLTKHAIERYETESFEAGGYKWKLVLYPNGNKSKNTKDHV 70

Query: 61  SLYLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLS 120
           S+YL+++   +  P GWEV  +FR  + DQ +DNYL +Q G  R++  +K E G    + 
Sbjct: 71  SVYLSLADSSSLSP-GWEVYAVFRLYLLDQNKDNYLILQ-GNERRFHSVKREWGFDKFIP 130

Query: 121 HNALNEASNGFVVDDSCTFGVEFSVLKV--SNKGESLSIIKEPQLDHFIWYIYPFSSYTG 180
               ++ASNG++++D+C FG +  V K   S +GE LS+IK+      +W I  FS    
Sbjct: 131 TGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATSSKHVWKIENFSKLDK 190

Query: 181 ESYTSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYN 240
           ESY S  F    RKW++  YP G+  G  T+LSIYL L + ETI  G KI+ ++ + I++
Sbjct: 191 ESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVEFTIRIFD 250

Query: 241 FGTKKYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARI 289
               ++       WF+ S+   G+ +++++      +   L  D   + A +
Sbjct: 251 QLQGRHIAGKVTKWFSRSSSEHGWVKYVSMVYFTQPNSGLLLKDVCLVEADV 300

BLAST of MS000276 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 163.3 bits (412), Expect = 3.0e-40
Identity = 97/294 (32.99%), Postives = 145/294 (49.32%), Query Frame = 0

Query: 2   RSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNG--DDKVEDHIS 61
           R  R E P+     I S+SV+   G E  ES  FE  GYKW+L+L+ NG  +D   DHIS
Sbjct: 52  RELREERPSSKIVTITSFSVIKGRG-EPYESSVFEAAGYKWRLVLYVNGNKNDGGNDHIS 111

Query: 62  LYLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSH 121
           LY  I  + N LPLGWEV V  +  + +     YLT+ DG V++Y+  K E G   L+  
Sbjct: 112 LYARI-EETNSLPLGWEVNVDLKLFVHNGKLHKYLTVTDGLVKRYNNAKKEWGFGQLIPR 171

Query: 122 NALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESY 181
           +    A+ G++  D+ +FG E  ++K + + E ++ I  P  + F W I  FS+   + Y
Sbjct: 172 STFYNANEGYLDQDTGSFGAEIFIVKPAQQQEKVTFISNPPNNVFTWKILRFSTLEDKFY 231

Query: 182 TSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGT 241
            S  F V+ R WR+   P G   G+   L I+L     +   +    +    L + N  +
Sbjct: 232 YSDDFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVATNTWGAVNLRLKNQRS 291

Query: 242 KKYFEKSDDHWF-TSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMS 293
             + +     W+   S YG G    + L DL D+SK YL  D +   A +V +S
Sbjct: 292 TNHRQIYSAAWYPIGSGYGVGVNNIILLADLNDASKGYLVNDAIIFEAEMVKVS 343

BLAST of MS000276 vs. TAIR 10
Match: AT5G26260.1 (TRAF-like family protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.1e-39
Identity = 94/294 (31.97%), Postives = 146/294 (49.66%), Query Frame = 0

Query: 2   RSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGDDK--VEDHIS 61
           R  R E P+     I S+SV+   G E  ES  FE  GYKW+L+L+  G+ K  + +HIS
Sbjct: 53  RELRDERPSSKIVTITSFSVIKDRG-EPYESSIFEAAGYKWRLVLYVKGNPKGGINNHIS 112

Query: 62  LYLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSH 121
           LY  I  +   LP GWEV V  +  + ++    YL++ DG V++Y+  K E G T L+S 
Sbjct: 113 LYARI-EETETLPRGWEVNVDLKLFVHNRKLKKYLSVTDGTVKRYNDAKKEWGFTQLISL 172

Query: 122 NALNEASNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESY 181
                A+ G++V D+ +FG E  ++  + K E ++ I  P  + F W I  FS+   + Y
Sbjct: 173 PTFYNANEGYLVQDTASFGAEIFIVNPTEKQEKVTFISNPPDNVFTWKILRFSTLEDKFY 232

Query: 182 TSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGT 241
            S  F V  R WR+   P GS  G+   L I+L     +   +    +    L + N  +
Sbjct: 233 YSDDFLVGDRYWRLGFNPKGSGGGRPHALPIFLYAQGHKANAVVTNTWGAVNLRLKNQRS 292

Query: 242 KKYFEKSDDHWF-TSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMS 293
             + +     W+   S YG G    + + +LKD+SK Y+  D +   A +V +S
Sbjct: 293 SNHKQLYSAAWYPIRSDYGVGVNNIILMSELKDASKGYMVNDAIIFEAEMVKVS 344

BLAST of MS000276 vs. TAIR 10
Match: AT3G20380.1 (TRAF-like family protein )

HSP 1 Score: 149.8 bits (377), Expect = 3.4e-36
Identity = 89/290 (30.69%), Postives = 147/290 (50.69%), Query Frame = 0

Query: 8   PPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNG--DDKVEDHISLYLAIS 67
           PP+ Y+FKI SY+   KI     ES  F   GY W L +HPNG   D    ++SLY+ + 
Sbjct: 85  PPSSYSFKIESYNSFLKIPYLGFESRPFAAGGYNWVLKVHPNGLTWDGTSGYVSLYVLL- 144

Query: 68  SKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLSHNALNEA 127
            +   +     V    RF IF+     Y T+QD  V K++  K   G   ++S +   + 
Sbjct: 145 HESTPITADQVVYADLRFYIFNNNEKKYFTVQDTNVWKFTAPKRLLGFPKVMSADQFEDL 204

Query: 128 SNGFVVDDSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGESYTSQPFT 187
            NG++ D+ C FGV+ +V     K ESL + ++     F + +  FS+   ESY S  F+
Sbjct: 205 RNGYIYDNHCEFGVDVTVASHYQKSESLFVTEKFDNPIFTYALLRFSTLLKESYQSDVFS 264

Query: 188 VKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNFGTKKYFEK 247
           + GR   ++++PNG ++ K   +S+YL +++       + IY +  L + N       E 
Sbjct: 265 IGGRSMYLQVFPNGRNLSKGKAMSLYLNINDK--FKPFEMIYVRAKLRVLNQRKLNNVEI 324

Query: 248 SDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLFLRARIVAMSTVE 296
              +W+TS  Y SG  + + L DL+DSSK ++  D L +  ++  +S+ +
Sbjct: 325 QVSNWYTSWFYYSGDFQIIPLADLRDSSKGFVVNDMLKVEVQLEGISSTK 371

BLAST of MS000276 vs. TAIR 10
Match: AT2G15710.1 (TRAF-like family protein )

HSP 1 Score: 146.4 bits (368), Expect = 3.8e-35
Identity = 87/286 (30.42%), Postives = 144/286 (50.35%), Query Frame = 0

Query: 1   IRSTRIEPPTHYTFKINSYSVLSKIGMEKCESGDFEVDGYKWKLILHPNGD--DKVEDHI 60
           + + R  PP+ Y+ KIN    LS++  +K ES  F   GY W+L+++P G+  DK    I
Sbjct: 90  VNNLREHPPSSYSLKINK---LSQLTFDKYESHRFLSGGYNWRLVIYPKGNEKDKGSGFI 149

Query: 61  SLYLAISSKGNQLPLGWEVRVIFRFLIFDQIRDNYLTIQDGKVRKYSKMKNEHGITHLLS 120
           S+Y+   +         EV     F ++++  + Y TIQD +V++++ ++   G++ +LS
Sbjct: 150 SMYVEFDNTKVSSTSPMEVFAYIIFFVYNKKENKYFTIQDVEVKRFNALRTVWGLSQVLS 209

Query: 121 HNALNEASNGFVVD-DSCTFGVEFSVLKVSNKGESLSIIKEPQLDHFIWYIYPFSSYTGE 180
               N+  NG+  + + C FGV+  V     K E +S  ++  +  F W +  FS    E
Sbjct: 210 LETFNDLENGYTFEGEQCEFGVDVMVASPITKWEVVSFDEKLDILKFSWSVKDFSVLKEE 269

Query: 181 SYTSQPFTVKGRKWRMRMYPNGSSVGKATNLSIYLKLDESETIPIGKKIYAKYFLGIYNF 240
            Y S+ F++ GR W ++MYP G        LSI+L+L  SET+ + +KIY    L + + 
Sbjct: 270 FYVSERFSMGGRLWDLQMYPKGDPRRDKKWLSIFLRLSGSETLTVDEKIYVIAHLRVLD- 329

Query: 241 GTKKYFEKSDDHWFTSSTYGSGFPEFLALKDLKDSSKSYLQTDGLF 284
                      +WF     G G+ EFL+   L+   KSYL  +  F
Sbjct: 330 --------PLGNWFRDRNKGWGYLEFLSFSKLR---KSYLDLEDTF 360