MS000268 (gene) Bitter gourd (TR) v1

BLAST of MS000268 vs. NCBI nr
Match: XP_022140813.1 (flavanone 3-dioxygenase 3 [Momordica charantia])

HSP 1 Score: 690.6 bits (1781), Expect = 6.8e-195
Identity = 353/381 (92.65%), Postives = 353/381 (92.65%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRSLAIEELREACIRLGFF---------------------------QIINHGINERVMEE 120
           QRSLAIEELREACIRLGFF                           QIINH INERVMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180
           ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240
           SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300
           CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 355
           SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of MS000268 vs. NCBI nr
Match: XP_004146935.2 (flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa_014422 [Cucumis sativus])

HSP 1 Score: 624.4 bits (1609), Expect = 6.0e-175
Identity = 306/354 (86.44%), Postives = 329/354 (92.94%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGL GESSG+FPTG++AQEKGLSYVPNAYVIPAPHR S S E AI+PIIDMASLRS+DS 
Sbjct: 3   MGLNGESSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSMSPEIAIVPIIDMASLRSSDSV 62

Query: 61  QRSLAIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRA 120
           QRSLA EELR+ACI LGFFQIINHGI E VMEEAL QA EFF+LPLKEKMKYKSDDV +A
Sbjct: 63  QRSLATEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLKEKMKYKSDDVCKA 122

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEV 180
           VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI SWPT+PR+YREKMG YT+EVRKV LE+
Sbjct: 123 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGRYTKEVRKVSLEI 182

Query: 181 MEAITESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSE 240
           MEAI ESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITT+LQSS+
Sbjct: 183 MEAINESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 242

Query: 241 GLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLE+MD ADGTWK+VP IDGALQVH+GDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 243 GLEVMDPADGTWKMVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 302

Query: 301 SLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKIQE 355
           SLGMDEKMKPAEELV++ NPKKYK+SSFNDFLKFLSSNDL EGKSF+NTLK+Q+
Sbjct: 303 SLGMDEKMKPAEELVNEHNPKKYKDSSFNDFLKFLSSNDLAEGKSFLNTLKLQD 356

BLAST of MS000268 vs. NCBI nr
Match: XP_008453850.1 (PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa] >TYK16900.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa])

HSP 1 Score: 620.9 bits (1600), Expect = 6.6e-174
Identity = 306/354 (86.44%), Postives = 327/354 (92.37%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGL GE SG+FPTG++AQEKGLSYVPNAYVIPAPHR S S E AI+PIIDMASLRS DS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRA 120
           QRSLA+EELR+ACI LGFFQIINHGI E VMEEAL QA EFF+LPL EKMKYKSDDV +A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWI SWPT+PR+YREKMG YT+EVRKV LE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSE 240
           MEAITESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITT+LQ+S+
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V  IDGALQVH+GDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKIQE 355
           SLGMDEKMKPAEELV++QNPKKYKESSFNDFLKFLSSNDL EG SFINTLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of MS000268 vs. NCBI nr
Match: XP_038889497.1 (flavanone 3-dioxygenase 3 [Benincasa hispida])

HSP 1 Score: 608.2 bits (1567), Expect = 4.5e-170
Identity = 300/350 (85.71%), Postives = 323/350 (92.29%), Query Frame = 0

Query: 5   GESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSAQRSL 64
           G  +GSFPTG++AQEKG SYVPNAYVIPAPHR+S S E A +PIIDMASLRS DSAQRSL
Sbjct: 9   GTGTGSFPTGKTAQEKGFSYVPNAYVIPAPHRASLSPEIATVPIIDMASLRSNDSAQRSL 68

Query: 65  AIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYG 124
           AI+ELR+ACI LGFFQIINHGI+E VMEEAL QA EFF+LPLKEKMKYKSDDV +AVRYG
Sbjct: 69  AIQELRKACISLGFFQIINHGISETVMEEALNQANEFFNLPLKEKMKYKSDDVRKAVRYG 128

Query: 125 TSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAI 184
           TSLKDGVDKIKFWRVFLKHYAHPLEDWI SWPT+PR+YREKMG YT+ VRKV LE+MEAI
Sbjct: 129 TSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKVVRKVSLEIMEAI 188

Query: 185 TESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSEGLEI 244
           TESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITT+LQS++GLEI
Sbjct: 189 TESLSLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSNQGLEI 248

Query: 245 MDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLGM 304
           MD ADGTWK+VP I GALQVHLGDHVEVLSNGLYKGVVHRATVNSE  RISITSLHSLGM
Sbjct: 249 MDPADGTWKMVPKIAGALQVHLGDHVEVLSNGLYKGVVHRATVNSERARISITSLHSLGM 308

Query: 305 DEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKIQE 355
           DEKMKPAEELVD+QNP KYK+SSF+DFLKFLSSNDL EGKSFINTLKI++
Sbjct: 309 DEKMKPAEELVDEQNPIKYKDSSFDDFLKFLSSNDLAEGKSFINTLKIED 358

BLAST of MS000268 vs. NCBI nr
Match: XP_023552054.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 605.9 bits (1561), Expect = 2.2e-169
Identity = 306/379 (80.74%), Postives = 330/379 (87.07%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGLGGESSGSFPTG++AQEKGL+YVPNAYVIP P R S S ETAI+PIIDMASLRS DSA
Sbjct: 62  MGLGGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSDDSA 121

Query: 61  QRSLAIEELREACIRLGFF-------------------------QIINHGINERVMEEAL 120
           QRSLAIE LR+ACI +GFF                         QIINHGI++ VMEEAL
Sbjct: 122 QRSLAIEVLRKACISIGFFQVKFQATYLPTSFILASSXLQFVFLQIINHGISKTVMEEAL 181

Query: 121 EQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIHSW 180
            QA EFF+LPLKEKMKYKSDDVS+AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+ SW
Sbjct: 182 SQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW 241

Query: 181 PTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPPCP 240
           PT+P +YREKMG YT+EVRKV LE+ EAITESLGL PTYLSSKMAEGVQVVAVNCYPPCP
Sbjct: 242 PTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCP 301

Query: 241 QPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSN 300
           QP VALGLPPHTDYSCITTILQSS+GLEIMD ADG WK+VPN+DG+LQVH+GDHVEVLSN
Sbjct: 302 QPGVALGLPPHTDYSCITTILQSSQGLEIMDPADGKWKMVPNVDGSLQVHVGDHVEVLSN 361

Query: 301 GLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFL 355
           GLYKGVVHRA VNSE TRISI+SLHSLGMDEKMKPAEELVD+QNP KYKESSFNDFLKFL
Sbjct: 362 GLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFL 421

BLAST of MS000268 vs. ExPASy Swiss-Prot
Match: Q7XR84 (Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.8e-81
Identity = 149/322 (46.27%), Postives = 212/322 (65.84%), Query Frame = 0

Query: 31  IPAPHRSSSSRETAIIPIIDMASLRSTDSAQRSLAIEELREACIRLGFFQIINHGINERV 90
           IP     S       +P+I++  L   D   RS  + ++ +AC  LG+FQ+I+HGI++ V
Sbjct: 8   IPQEQLPSQELHPPPMPVINLGHLSLDDPTVRSRVVNDIAKACRDLGYFQVISHGISQSV 67

Query: 91  MEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLED 150
           M+ A+E A EFF LP + K +Y SDD+ + VRY TS KDG   I   R FLKHYAHPL D
Sbjct: 68  MDGAIEAASEFFKLPNEIKKEYASDDIRQPVRYDTSSKDG---ISMSRAFLKHYAHPLCD 127

Query: 151 WIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNC 210
           W+  WP  P  YRE M  Y  EVR V L++MEAI E LG+   Y+  K  EG+Q+++VNC
Sbjct: 128 WLQYWPQQPPIYREYMAKYAVEVRVVALKLMEAILEGLGIGKEYMQEKFEEGLQLLSVNC 187

Query: 211 YPPCPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHV 270
           YP   Q + ++GL  H+DY  +T +L S +GLE++D +  +WK+V  +  AL VH+GDH+
Sbjct: 188 YPKVSQSDTSIGLAAHSDYGLLTILLTSCQGLEVVDRSSNSWKVVQQLPHALHVHVGDHM 247

Query: 271 EVLSNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFND 330
           EVLSNG  K VVHRA +N +  RIS+ S+H   + EK+  A+ELVD++NP+KYKE+SFND
Sbjct: 248 EVLSNGRIKTVVHRAVLNPQEARISLASIHGFALHEKVSSAKELVDEENPQKYKENSFND 307

Query: 331 FLKFLSSNDLGEGKSFINTLKI 353
           FL+ L++N     ++F+ +L++
Sbjct: 308 FLEHLTANMDNRQRNFLESLRM 326

BLAST of MS000268 vs. ExPASy Swiss-Prot
Match: Q7XR83 (2-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD21 PE=1 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.2e-79
Identity = 152/310 (49.03%), Postives = 208/310 (67.10%), Query Frame = 0

Query: 46  IPIIDMASLRSTDSAQRSLAIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLP 105
           +P+I++  L + DSA R+LA+ ++  AC   G F+++NHGI+   M  ALE A EFF L 
Sbjct: 28  LPVINLGRL-TMDSASRALAVRDIVLACRERGCFEVVNHGISRSCMNGALEAASEFFQLS 87

Query: 106 LKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREK 165
            + K ++ SDD+ + +RY TS +DG   I   R FLKHYA+PL+DWI  WPT P +YREK
Sbjct: 88  TERKEEFASDDIRQPIRYDTSSRDG---ISMSRSFLKHYANPLDDWIKFWPTQPPTYREK 147

Query: 166 MGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEV-ALGLP 225
           MG Y  E ++V +++MEAI + LGL P+YL  K+  GVQ VA+N YP     +   +GL 
Sbjct: 148 MGEYAVETQRVSMQLMEAILQGLGLGPSYLQEKLEGGVQFVALNNYPQSSAKKADKIGLA 207

Query: 226 PHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHR 285
           PH+DY  +T +LQSS GLE+M   D  W  VP I GAL VH+GDH+EVLSNG  K +VHR
Sbjct: 208 PHSDYGFLTILLQSSPGLEVMHHEDDAWTSVPAIPGALHVHVGDHLEVLSNGQLKSLVHR 267

Query: 286 ATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGK 345
           A +N   +RISI S+H L MDE++  AEELVD+ +PK Y+ SSF DFL FL +N +   K
Sbjct: 268 AVLNPNESRISIASIHGLSMDEEVHCAEELVDEHHPKMYRGSSFQDFLDFLPAN-MNRYK 327

Query: 346 SFINTLKIQE 355
            ++ +L+I +
Sbjct: 328 RYVESLRIDK 332

BLAST of MS000268 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.1e-66
Identity = 133/342 (38.89%), Postives = 200/342 (58.48%), Query Frame = 0

Query: 19  EKGLSYVPNAYVIPAPHRSSSSRETAI-------IPIIDMASLRSTDSAQRSLAIEELRE 78
           + G++ +P  YV+PA  R   +   A        +P++D++ LR    ++R   +  L  
Sbjct: 46  DSGITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRV--PSERGAVLRTLDA 105

Query: 79  ACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGV 138
           AC   GFFQ++NHG+   V+   L+ A  FF+LP  E+ +Y S DV   VRYGTS     
Sbjct: 106 ACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVR 165

Query: 139 DKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLC 198
           D +  WR FLK    PL   + SWPTSP   RE    Y    ++V +EVMEA  E+LG+ 
Sbjct: 166 DAVLCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVG 225

Query: 199 PTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSS-EGLEIMDMADG 258
              +   +A G Q++ VNCYP CPQPE+ LG+PPH+DY  +T +LQ    GL++M    G
Sbjct: 226 GGGVMEDLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHA--G 285

Query: 259 TWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKP 318
            W  V  + G+  V++GDH+E+LSNG Y+ V+HR  VNS   R+S+ S HS+  +  + P
Sbjct: 286 EWLTVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASFHSVAPERVVSP 345

Query: 319 AEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKI 353
           A EL+D ++P++Y ++    FL +L+S   G  KSF+++ ++
Sbjct: 346 APELIDDRHPRRYMDTDLATFLAYLAS-AAGNHKSFLHSRRL 382

BLAST of MS000268 vs. ExPASy Swiss-Prot
Match: Q651B6 (Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC2 PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 2.7e-61
Identity = 138/360 (38.33%), Postives = 206/360 (57.22%), Query Frame = 0

Query: 5   GESSGSFPTGESAQ---EKGLSYVPNAYVIPAPHRSSS-SRETAIIPIIDMASLRSTDSA 64
           GE SGS       +   + G++ +P  YV+P   R +      A++P++D+A+LR+ D  
Sbjct: 13  GEGSGSSEWELGVRQLCDSGITTLPARYVLPPADRPARYVTPPALLPVVDLAALRARDPC 72

Query: 65  QRSLAIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRA 124
           Q    +  L  AC   GFFQ++NHG+    M  A  +   FFDLPL  + +Y S D+  A
Sbjct: 73  Q----LAALHAACRDYGFFQLLNHGVPPDAMLYAARRF--FFDLPLPARKRYMSADIRAA 132

Query: 125 VRYGTSLKDGVDKIKFWRVFLK---HYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVG 184
           VRYGTS     D +  WR FLK        L D + SWP +P   R     Y    +++ 
Sbjct: 133 VRYGTSFNQLNDAVLSWRDFLKLLIRDTRRLADVLPSWPDAPDDLRPAAAAYATACQRLF 192

Query: 185 LEVMEAITESLGL--CPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTI 244
            E+MEA  ++LG+  C   L  +   G Q++ VNC+P CP+PE+ LG+PPH+DY  +T +
Sbjct: 193 RELMEAALDALGIVRCRRQLLEECDAGSQMMMVNCFPACPEPELTLGMPPHSDYGLLTIL 252

Query: 245 LQSS-EGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRI 304
           LQ    GLE+     G W +V  + GA+ V++GDH+E+LSNGLY+ V+HR  VN    R+
Sbjct: 253 LQDEVRGLEVSYGDGGGWAVVEPLPGAVVVNVGDHLEILSNGLYRSVLHRVRVNGRRARV 312

Query: 305 SITSLHSLGMDEKMKPAEELVDQQ--NPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKI 353
           S+ SLHSL  +  + PA ELVD+Q   P++Y ++    FL +L+S + G  KSF+++ +I
Sbjct: 313 SVASLHSLAAERVIGPAAELVDEQRGRPRRYMDTDMAAFLAYLASAE-GNHKSFLHSRRI 365

BLAST of MS000268 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.4e-59
Identity = 118/331 (35.65%), Postives = 192/331 (58.01%), Query Frame = 0

Query: 25  VPNAYVIPAPHRSSSSRETAI--IPIIDMASLRSTDSAQRSLAIEELREACIRLGFFQII 84
           +P  YV P   R   S  + +   P+ID++      S  RS  I+++ +AC R GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG+N+++++E +  A EFF + ++EKMK  SDD ++  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEG 204
            + +P+  +++ WP++P S++E +  Y+REVR+VG ++ E I+ESLGL   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGAL 264
            Q +AVN YPPCP+PE+  GLP HTD + +T +LQ +    +  + DG W  V     A 
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 254

Query: 265 QVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSL---HSLGMDEKMKPAEELVDQQN 324
            +++GD ++ LSNG+YK V HRA  N+E+ R+S+ S        +    KP  E  D + 
Sbjct: 255 VINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDET 314

Query: 325 PKKYKESSFNDFLKFLSSNDLGEGKSFINTL 351
              YK+ ++ ++ K   S +L +     N L
Sbjct: 315 KPVYKDFTYAEYYKKFWSRNLDQEHCLENFL 339

BLAST of MS000268 vs. ExPASy TrEMBL
Match: A0A6J1CIV6 (flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.3e-195
Identity = 353/381 (92.65%), Postives = 353/381 (92.65%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  QRSLAIEELREACIRLGFF---------------------------QIINHGINERVMEE 120
           QRSLAIEELREACIRLGFF                           QIINH INERVMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180
           ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240
           SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300
           CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 355
           SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of MS000268 vs. ExPASy TrEMBL
Match: A0A5A7TMH3 (Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00340 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.2e-174
Identity = 306/354 (86.44%), Postives = 327/354 (92.37%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGL GE SG+FPTG++AQEKGLSYVPNAYVIPAPHR S S E AI+PIIDMASLRS DS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRA 120
           QRSLA+EELR+ACI LGFFQIINHGI E VMEEAL QA EFF+LPL EKMKYKSDDV +A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWI SWPT+PR+YREKMG YT+EVRKV LE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSE 240
           MEAITESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITT+LQ+S+
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V  IDGALQVH+GDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKIQE 355
           SLGMDEKMKPAEELV++QNPKKYKESSFNDFLKFLSSNDL EG SFINTLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of MS000268 vs. ExPASy TrEMBL
Match: A0A1S3BX86 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.2e-174
Identity = 306/354 (86.44%), Postives = 327/354 (92.37%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGL GE SG+FPTG++AQEKGLSYVPNAYVIPAPHR S S E AI+PIIDMASLRS DS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  QRSLAIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRA 120
           QRSLA+EELR+ACI LGFFQIINHGI E VMEEAL QA EFF+LPL EKMKYKSDDV +A
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEV 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDWI SWPT+PR+YREKMG YT+EVRKV LE+
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 MEAITESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSE 240
           MEAITESL L PTYLSSKMAEGVQVVAVNCYPPCPQP VALGLPPHTDYSCITT+LQ+S+
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLH 300
           GLEIMD ADGTWK+V  IDGALQVH+GDHVEVLSNGLYKGVVHRATVNSE TRISITSLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKIQE 355
           SLGMDEKMKPAEELV++QNPKKYKESSFNDFLKFLSSNDL EG SFINTLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of MS000268 vs. ExPASy TrEMBL
Match: A0A6J1E3T8 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430549 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.6e-168
Identity = 304/376 (80.85%), Postives = 327/376 (86.97%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGL GESSGSF TG++AQEKGL+YVPNAYVIP P R S S ETAI+PIIDMASLRS DSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELREACIRLGFF----------------------QIINHGINERVMEEALEQA 120
           QRSL IE LR+ACI LGFF                      QIINHGI++ VMEEAL QA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 YEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTS 180
            EFF+LPLKEKMKYKSDDVS+AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+ SWPT+
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPE 240
           P +YREKMG YT+EVRKV LE+ EAITESLGL PTYLSSKMAEGVQVVAVNCYPPCPQP 
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVLSNGLY 300
           VALGLPPHTDYSCITTILQSS+GLEIMD ADG WK+VPN+DG+LQVH+GDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSN 355
           KGVVHRA VNSE TRISI+SLHSLGMDEKMKPAEELVD+QNP KYKESSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of MS000268 vs. ExPASy TrEMBL
Match: A0A6J1JAE6 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LOC111482715 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.6e-165
Identity = 303/381 (79.53%), Postives = 326/381 (85.56%), Query Frame = 0

Query: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60
           MGL GESSGSF TG++AQEKGL+YVPNAYVIP P R S S ETAI+PIIDMASLRS DSA
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  QRSLAIEELREACIRLGFF----------------------------QIINHGINERVME 120
           +RSLAIE LR+ACI LGFF                            QIINHGI++ VME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWI 180
           EAL QA EFF+LPLKEKMKYKSDDVS+AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 HSWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYP 240
            SWPT+P +YREKMG YT+EVRKV LE+ EAITESLGL P YLSSKMAEGVQVVAVNCYP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYP 240

Query: 241 PCPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEV 300
           PCPQP VALGLPPHTDYSCITTILQSS+GLEIMD ADG WK+VPN+DG+LQVH+GDHVEV
Sbjct: 241 PCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEV 300

Query: 301 LSNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFL 354
           LSNGLYKGVVHRA VNSE TRISI+SLHSLGMDEKMKPAEELVD+QNP KYKESSFNDFL
Sbjct: 301 LSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFL 360

BLAST of MS000268 vs. TAIR 10
Match: AT5G07480.1 (KAR-UP oxidoreductase 1 )

HSP 1 Score: 396.7 bits (1018), Expect = 1.9e-110
Identity = 196/355 (55.21%), Postives = 249/355 (70.14%), Query Frame = 0

Query: 5   GESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSAQRSL 64
           GE S +F  G SAQE+ L YVP+ YV+P P        + I+P ID++ L+  D  +R  
Sbjct: 4   GEDSSTFKMGNSAQERSLPYVPDCYVVP-PSSKPCDSNSGIVPTIDVSRLKGGDDERRG- 63

Query: 65  AIEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYG 124
                           I+NHGIN+ ++++ALE A  FF+LP KEK ++ S+DV   VRY 
Sbjct: 64  ----------------IVNHGINQNILDDALEVANSFFELPAKEKKQFMSNDVYAPVRYS 123

Query: 125 TSLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAI 184
           TSLKDG+D I+FWR+FLKHYAHPL  WIH WP +P  YREKMG +  EVRK+ +E+M AI
Sbjct: 124 TSLKDGLDTIQFWRIFLKHYAHPLHRWIHLWPENPPGYREKMGKFCEEVRKLSIELMGAI 183

Query: 185 TESLGLCPTYLSSKMAE-GVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSEGLE 244
           TESLGL   YLSS+M E G+QV+ VNCYPPCP PE ALGLPPH+DYSCIT +LQ+ +GL+
Sbjct: 184 TESLGLGRDYLSSRMDENGMQVMTVNCYPPCPDPETALGLPPHSDYSCITLLLQNLDGLK 243

Query: 245 IMDM----ADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSL 304
           I D       G W  VP + G L+VH+GDHVEVLSNGLYK +VH+ T+N E TRIS+ SL
Sbjct: 244 IFDPMAHGGSGRWVGVPQVTGVLKVHIGDHVEVLSNGLYKSIVHKVTLNEEKTRISLASL 303

Query: 305 HSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKIQE 355
           HSLGMD+KM    ELV+ +NP +YKESSFNDFL FL  ND+ +G  FI+TL+I++
Sbjct: 304 HSLGMDDKMSVPRELVNDENPVRYKESSFNDFLDFLVKNDISQGDRFIDTLRIKD 340

BLAST of MS000268 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 333.2 bits (853), Expect = 2.6e-91
Identity = 169/346 (48.84%), Postives = 231/346 (66.76%), Query Frame = 0

Query: 10  SFPTGESAQEKGLSYVPNAYVIPAPHR----SSSSRETAIIPIIDMASLRSTDSAQRSLA 69
           SF +  +    G+  VP+ YV+P   R    SS       +P+ID++ L       RSLA
Sbjct: 12  SFTSAMTLTNSGVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLH--QPFLRSLA 71

Query: 70  IEELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGT 129
           I E+  AC   GFFQ+INHGI   V+ +AL+ A +FFDLP++EKM   S +V   VRYGT
Sbjct: 72  IHEISMACKEFGFFQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGT 131

Query: 130 SLKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAIT 189
           SL    D++ +WR F+KHY+HPL  WI  WP++P  Y++K+G Y      +  +++EAI+
Sbjct: 132 SLNHSTDRVHYWRDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAIS 191

Query: 190 ESLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSEGLEIM 249
           ESLGL   YL  ++ EG QV+AVNCYP CP+PE+ALG+PPH+D+S +T +LQSS+GL+IM
Sbjct: 192 ESLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIM 251

Query: 250 DMADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLGMD 309
           D  +  W  VP I+GAL V LGD VEV+SNG+YK V+HR TVN E  R+S  SLHSL + 
Sbjct: 252 D-CNKNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLH 311

Query: 310 EKMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLK 352
           +K+ PA +LV+  N   Y E SFNDFL ++SSND  + + FI+T+K
Sbjct: 312 KKISPAPKLVNPNNAPAYGEFSFNDFLNYISSNDFIQ-ERFIDTIK 353

BLAST of MS000268 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.5e-91
Identity = 166/345 (48.12%), Postives = 226/345 (65.51%), Query Frame = 0

Query: 11  FPTGESAQEKGLSYVPNAYVIPAPHR----SSSSRETAIIPIIDMASLRSTDSAQRSLAI 70
           F +     E G  +VP  Y++P   R     S    T  +P+ID++ L   D   R   I
Sbjct: 13  FTSAMELTESGDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLH--DPLLRLCVI 72

Query: 71  EELREACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTS 130
            E+  AC   GFFQ+INHGI+  V+++A + A  FFDLP  EKM   SD+    VRYGTS
Sbjct: 73  HEIELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVRYGTS 132

Query: 131 LKDGVDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAITE 190
           +    D++ +WR F+KHY+HPL +WI+ WP++P  Y+EK+G Y      +  +++EAI+E
Sbjct: 133 INHSTDRVHYWRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISE 192

Query: 191 SLGLCPTYLSSKMAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSEGLEIMD 250
           SLGL   YL  ++ EG QV+AVNCYP CP+PE+ALG+PPH+DY  +T +LQSSEGL+I D
Sbjct: 193 SLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKD 252

Query: 251 MADGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLGMDE 310
             +  W  VP I+GAL V LGD VEV+SNG+YK VVHR TVN +  R+S  SLHSL M +
Sbjct: 253 -CNNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHK 312

Query: 311 KMKPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLK 352
           K+ PA +LV++  P  Y E SFNDFL ++S ND+ + K FI+TL+
Sbjct: 313 KISPATQLVNENKPAAYGEFSFNDFLDYISRNDITQ-KRFIDTLR 353

BLAST of MS000268 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 267.3 bits (682), Expect = 1.7e-71
Identity = 141/344 (40.99%), Postives = 210/344 (61.05%), Query Frame = 0

Query: 19  EKGLSYVPNAYVIPAPHRSSSSRETAII--------PIIDMASLRSTDSAQRSLAIEELR 78
           E GL+ VP  Y+ P P R   ++   +I        P+ID A L   +   R   +  + 
Sbjct: 26  ENGLTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAELLGPN---RPHVLRTIA 85

Query: 79  EACIRLGFFQIINHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDG 138
           EAC   GFFQ++NHG+   V +  ++    FF+LP +E+ KY S D+S  VRYGTS    
Sbjct: 86  EACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYGTSFNQI 145

Query: 139 VDKIKFWRVFLKHYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGL 198
            D +  WR FLK YAHPL D++  WP+SP  +R    TY +E +++   +++AI ESL +
Sbjct: 146 KDNVFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAILESLEI 205

Query: 199 CPTYLSSK-MAEGVQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSS-EGLEIMDMA 258
             +  ++K + EG QVV VNCYPPCP+PE+ LG+PPH+DY  +T +LQ   EGL+I+   
Sbjct: 206 DGSDEAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEGLQILYRD 265

Query: 259 DGTWKLVPNIDGALQVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSLHSLGMDEKM 318
           +  W  V  I G+  V++GDH+E+ SNG YK V+HR  VNS   RIS+ SLHS  +   +
Sbjct: 266 E--WVTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLVNSTKPRISVASLHSFPLTSVV 325

Query: 319 KPAEELVDQQNPKKYKESSFNDFLKFLSSNDLGEGKSFINTLKI 353
           KP+ +LVD+ NP +Y ++ F  FL++++S +  + K+F+ + KI
Sbjct: 326 KPSPKLVDKHNPSQYMDTDFTTFLQYITSRE-PKWKNFLESRKI 363

BLAST of MS000268 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 5.2e-60
Identity = 118/331 (35.65%), Postives = 192/331 (58.01%), Query Frame = 0

Query: 25  VPNAYVIPAPHRSSSSRETAI--IPIIDMASLRSTDSAQRSLAIEELREACIRLGFFQII 84
           +P  YV P   R   S  + +   P+ID++      S  RS  I+++ +AC R GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGINERVMEEALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG+N+++++E +  A EFF + ++EKMK  SDD ++  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWIHSWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEG 204
            + +P+  +++ WP++P S++E +  Y+REVR+VG ++ E I+ESLGL   Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGAL 264
            Q +AVN YPPCP+PE+  GLP HTD + +T +LQ +    +  + DG W  V     A 
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAF 254

Query: 265 QVHLGDHVEVLSNGLYKGVVHRATVNSESTRISITSL---HSLGMDEKMKPAEELVDQQN 324
            +++GD ++ LSNG+YK V HRA  N+E+ R+S+ S        +    KP  E  D + 
Sbjct: 255 VINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDET 314

Query: 325 PKKYKESSFNDFLKFLSSNDLGEGKSFINTL 351
              YK+ ++ ++ K   S +L +     N L
Sbjct: 315 KPVYKDFTYAEYYKKFWSRNLDQEHCLENFL 339