MS000208 (gene) Bitter gourd (TR) v1

Overview
NameMS000208
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold251: 340096 .. 340674 (-)
RNA-Seq ExpressionMS000208
SyntenyMS000208
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGATTTTTTGGAAACAACGCTTTAGGGAAGACTGGCTCAAATGGGGAGATTGTAACACTAAGTGGTTTCACAAAAAGGCCACCATGCGAAAGTCTTGTAATTCTATTCATGTCATTGAGAACCAAAATGGGTGCCGGACAGAAGAGTTGGAGGAGATTGAGAATACTTTTGTAGCTTATTTCCAATCTATTTTCACCTCTTCTTCTCCCTCATCATCGGATATTGAAGCAATTACAAATCTTATTCCCACAAGGATTACTCCGGAGATAAATGTCTGGCTTTTGGCACCTTATAACCAAGAGGAAATAGAGAATGCCATTAAACAAATGTTTCCAACGAAAACTCCTGGCCCTGATGGTTTTCCTACTCTTTTCTACCAGACTTATTGCCATGTAGTGGGACCTAAAACAGTAGAGGCTTGTCTTAATGTTCTGAACAACGATGGTGATATCCGAAACTGGAATACTACGTATATTGCTCTTATTCCGAAGGTCAAACAACCAGGGTCAGTTTTTGATTTTCGTCCCATAAGTCTGTGCAACGTATCTTATAAGATTATTTCTAAATCAATCACTAAT

mRNA sequence

GAGATTTTTTGGAAACAACGCTTTAGGGAAGACTGGCTCAAATGGGGAGATTGTAACACTAAGTGGTTTCACAAAAAGGCCACCATGCGAAAGTCTTGTAATTCTATTCATGTCATTGAGAACCAAAATGGGTGCCGGACAGAAGAGTTGGAGGAGATTGAGAATACTTTTGTAGCTTATTTCCAATCTATTTTCACCTCTTCTTCTCCCTCATCATCGGATATTGAAGCAATTACAAATCTTATTCCCACAAGGATTACTCCGGAGATAAATGTCTGGCTTTTGGCACCTTATAACCAAGAGGAAATAGAGAATGCCATTAAACAAATGTTTCCAACGAAAACTCCTGGCCCTGATGGTTTTCCTACTCTTTTCTACCAGACTTATTGCCATGTAGTGGGACCTAAAACAGTAGAGGCTTGTCTTAATGTTCTGAACAACGATGGTGATATCCGAAACTGGAATACTACGTATATTGCTCTTATTCCGAAGGTCAAACAACCAGGGTCAGTTTTTGATTTTCGTCCCATAAGTCTGTGCAACGTATCTTATAAGATTATTTCTAAATCAATCACTAAT

Coding sequence (CDS)

GAGATTTTTTGGAAACAACGCTTTAGGGAAGACTGGCTCAAATGGGGAGATTGTAACACTAAGTGGTTTCACAAAAAGGCCACCATGCGAAAGTCTTGTAATTCTATTCATGTCATTGAGAACCAAAATGGGTGCCGGACAGAAGAGTTGGAGGAGATTGAGAATACTTTTGTAGCTTATTTCCAATCTATTTTCACCTCTTCTTCTCCCTCATCATCGGATATTGAAGCAATTACAAATCTTATTCCCACAAGGATTACTCCGGAGATAAATGTCTGGCTTTTGGCACCTTATAACCAAGAGGAAATAGAGAATGCCATTAAACAAATGTTTCCAACGAAAACTCCTGGCCCTGATGGTTTTCCTACTCTTTTCTACCAGACTTATTGCCATGTAGTGGGACCTAAAACAGTAGAGGCTTGTCTTAATGTTCTGAACAACGATGGTGATATCCGAAACTGGAATACTACGTATATTGCTCTTATTCCGAAGGTCAAACAACCAGGGTCAGTTTTTGATTTTCGTCCCATAAGTCTGTGCAACGTATCTTATAAGATTATTTCTAAATCAATCACTAAT

Protein sequence

EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAYFQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDGFPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLCNVSYKIISKSITN
Homology
BLAST of MS000208 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 212.2 bits (539), Expect = 3.9e-51
Identity = 112/193 (58.03%), Postives = 123/193 (63.73%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           EIFWKQR REDWLKWG                                         +A 
Sbjct: 543 EIFWKQRSREDWLKWG-----------------------------------------IAI 602

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
             ++         DIEAI NLIPTRIT E+N  LLAPY +EEIE AI+QMFPTK  GPDG
Sbjct: 603 LNAL---------DIEAIINLIPTRITSEVNEQLLAPYTKEEIELAIRQMFPTKALGPDG 662

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
           FP LFYQTY HVVGPKT+EACLN LNN  DI+ WN+TYIALIPK+KQP S+ DFRPISLC
Sbjct: 663 FPALFYQTYWHVVGPKTLEACLNALNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLC 685

Query: 181 NVSYKIISKSITN 194
           NVSYKIISKSITN
Sbjct: 723 NVSYKIISKSITN 685

BLAST of MS000208 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 192.2 bits (487), Expect = 4.2e-45
Identity = 92/194 (47.42%), Postives = 131/194 (67.53%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           E++WKQR R DWLK GD NTK+FH KA+ R+  N I  +E+  G   ++ E IE  F  +
Sbjct: 426 EVYWKQRSRADWLKEGDKNTKFFHSKASARRRKNKIWGVEDDQGNWVDDPEGIEGEFCGF 485

Query: 61  FQSIFTSSSPSSSDI-EAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPD 120
           FQ +FTSS+PS + I EA+  L+P +++ E+N  L  P+  E+I  A+ +M PTK PGPD
Sbjct: 486 FQQLFTSSNPSQTQISEALKGLLP-KVSQEMNTHLEEPFTPEDITRALSEMCPTKAPGPD 545

Query: 121 GFPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISL 180
           G P  F+Q +  +VG    + CL++LN  G + + N T+IALIPKV++P  V +FRPISL
Sbjct: 546 GLPAAFFQKHWQIVGEGLTKTCLHILNEQGTLDSLNHTFIALIPKVEKPRKVMEFRPISL 605

Query: 181 CNVSYKIISKSITN 194
           CNV Y+I++K+I N
Sbjct: 606 CNVVYRIVAKAIAN 618

BLAST of MS000208 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 190.3 bits (482), Expect = 1.6e-44
Identity = 91/193 (47.15%), Postives = 125/193 (64.77%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           ++F KQR R DWL+ GD NTK+FH KA+ RK  N +  IEN  G   +E +EIE    ++
Sbjct: 394 KVFKKQRSRADWLREGDKNTKYFHAKASARKRKNKVWGIENDQGIWLKERDEIEEEICSH 453

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
           FQ +FT+SSP+ S I+A    +  ++TPE+NV L  P+  E++E A+  M PTK PGPDG
Sbjct: 454 FQELFTTSSPTPSQIQATLQGMSAKVTPEMNVQLEKPFTAEDVEEALANMCPTKAPGPDG 513

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
            P  F+Q +   V    +  CLNVLN  G+    N T+IALIPK+  P  V D+RPISLC
Sbjct: 514 LPAAFFQKHWKSVRNGVISTCLNVLNEQGNPSTLNHTFIALIPKIANPRKVSDYRPISLC 573

Query: 181 NVSYKIISKSITN 194
           NV Y++++K+I N
Sbjct: 574 NVIYRVVAKAIAN 586

BLAST of MS000208 vs. NCBI nr
Match: XP_042952177.1 (uncharacterized protein LOC122289261 [Carya illinoinensis])

HSP 1 Score: 184.9 bits (468), Expect = 6.6e-43
Identity = 89/190 (46.84%), Postives = 128/190 (67.37%), Query Frame = 0

Query: 4   WKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAYFQS 63
           WKQR ++ WLK GD N+ +FHK A  R+  NSI  +   +G  T ++ EI + F  YFQ 
Sbjct: 260 WKQRAKQMWLKDGDRNSNYFHKCANQRRKNNSIQKLVRNDGLTTTDIGEISSMFTTYFQE 319

Query: 64  IFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDGFPT 123
           +F+S+SP+S D+    N +  R+  E+N  L   +  EE+++A+ QM P   PGPDGFP 
Sbjct: 320 LFSSASPNSIDV--CLNQVHHRVNSEMNSNLTKQFTAEEVKSALFQMNPMGAPGPDGFPA 379

Query: 124 LFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLCNVS 183
           LFYQ++  VVG +  +A L++LN +G+I + N TYI LIPKVK P +V DF+PISLCNV 
Sbjct: 380 LFYQSHWDVVGEEVTKAVLDILNGNGEISHINETYIVLIPKVKNPQTVMDFKPISLCNVI 439

Query: 184 YKIISKSITN 194
           YK++SK+++N
Sbjct: 440 YKVVSKTLSN 447

BLAST of MS000208 vs. NCBI nr
Match: XP_024038343.1 (uncharacterized protein LOC112097373 [Citrus clementina])

HSP 1 Score: 184.5 bits (467), Expect = 8.7e-43
Identity = 93/193 (48.19%), Postives = 121/193 (62.69%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           EI+WKQR R DWLK GD NTK+FH KA+ RK  N I  IEN  G   E  E +E  F  Y
Sbjct: 301 EIYWKQRSRADWLKGGDKNTKFFHHKASSRKKKNRIWGIENAAGNWIENAEGVEFEFNKY 360

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
           F ++FT+S P+   I A  + I  R++ E+N  L  P+  EE+  A+ QM PTK PGPDG
Sbjct: 361 FTNLFTTSKPNQDQIAAALSGISRRVSTEMNESLEMPFTPEEVVEALTQMCPTKAPGPDG 420

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
            P +F+Q +   V    +  CL++LN  GD+  +N TYI LI K  +P  V DFRPISLC
Sbjct: 421 LPAVFFQKHWQRVKQGVLSTCLHILNKQGDVAPFNHTYIVLISKKGKPRKVTDFRPISLC 480

Query: 181 NVSYKIISKSITN 194
           NV Y+I++K+I N
Sbjct: 481 NVIYRIVAKAIAN 493

BLAST of MS000208 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 3.3e-13
Identity = 51/164 (31.10%), Postives = 87/164 (53.05%), Query Frame = 0

Query: 36  IHVIENQNGCRTEELEEIENTFVAYFQSIFTSSSPSSSDIEAITNLIPTRITPEINV--- 95
           I+ I N+ G  T + EEI+NT  ++++ ++   S    +++ +   +     P++N    
Sbjct: 397 INKIRNEKGDITTDPEEIQNTIRSFYKRLY---STKLENLDEMDKFLDRYQVPKLNQDQV 456

Query: 96  -WLLAPYNQEEIENAIKQMFPTKTPGPDGFPTLFYQTYCHVVGPKTVEACLNVLNNDGDI 155
             L +P + +EIE  I  +   K+PGPDGF   FYQT+   + P  +    + +  +G +
Sbjct: 457 DHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIP-ILHKLFHKIEVEGTL 516

Query: 156 RN-WNTTYIALIPK-VKQPGSVFDFRPISLCNVSYKIISKSITN 194
            N +    I LIPK  K P  + +FRPISL N+  KI++K + N
Sbjct: 517 PNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILAN 556

BLAST of MS000208 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 9.1e-11
Identity = 52/171 (30.41%), Postives = 85/171 (49.71%), Query Frame = 0

Query: 28  TMRKSCNS-IHVIENQNGCRTEELEEIENTFVAYFQSIFTSSSPSSSDIEAITNL--IPT 87
           T +K   S I  I N N   T +  EI+     Y++ +++    +  +I+       +P 
Sbjct: 380 TRKKRVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYENLKEIDQYLEACHLPR 439

Query: 88  RITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDGFPTLFYQTYCHVVGPKTVEACLNV 147
               E+ + L  P +  EI + I+ +   K+PGPDGF + FYQT+   + P  +    N+
Sbjct: 440 LSQKEVEM-LNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLFQNI 499

Query: 148 LNNDGDIRN-WNTTYIALIPKV-KQPGSVFDFRPISLCNVSYKIISKSITN 194
              +G + N +    I LIPK  K P    ++RPISL N+  KI++K +TN
Sbjct: 500 -EKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTN 548

BLAST of MS000208 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.0e-09
Identity = 49/170 (28.82%), Postives = 79/170 (46.47%), Query Frame = 0

Query: 30  RKSCNSIHVIENQNGCRTEELEEIENTFVAYFQSIFTSSSPSSSDIEAITNLIPTRITPE 89
           ++  N I  I+N  G  T +  EI+ T   Y++ ++ +      ++E +   + T   P 
Sbjct: 384 KREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYAN---KLENLEEMDTFLDTYTLPR 443

Query: 90  IN----VWLLAPYNQEEIENAIKQMFPTKTPGPDGFPTLFYQTYCHVVGPKTVEACLNVL 149
           +N      L  P    EI   I  +   K+PGPDGF   FYQ Y   + P  ++     +
Sbjct: 444 LNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLK-LFQSI 503

Query: 150 NNDGDIRN-WNTTYIALIPKV-KQPGSVFDFRPISLCNVSYKIISKSITN 194
             +G + N +    I LIPK  +      +FRPISL N+  KI++K + N
Sbjct: 504 EKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILAN 549

BLAST of MS000208 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.4e-08
Identity = 38/176 (21.59%), Postives = 81/176 (46.02%), Query Frame = 0

Query: 17  DCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAYFQSIFTSSSPSSSDIE 76
           D  +++F+     + +   I  +  ++G   E+ E I +   +++Q++F+    S    E
Sbjct: 369 DRGSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDRARSFYQNLFSPDPISPDACE 428

Query: 77  AITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDGFPTLFYQTYCHVVGPK 136
            + + +P  ++      L  P   +E+  A++ M   K+PG DG    F+Q +   +GP 
Sbjct: 429 ELWDGLPV-VSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPD 488

Query: 137 TVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLCNVSYKIISKSIT 193
                           +     ++L+PK      + ++RP+SL +  YKI++K+I+
Sbjct: 489 FHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAIS 543

BLAST of MS000208 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.9e-51
Identity = 112/193 (58.03%), Postives = 123/193 (63.73%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           EIFWKQR REDWLKWG                                         +A 
Sbjct: 543 EIFWKQRSREDWLKWG-----------------------------------------IAI 602

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
             ++         DIEAI NLIPTRIT E+N  LLAPY +EEIE AI+QMFPTK  GPDG
Sbjct: 603 LNAL---------DIEAIINLIPTRITSEVNEQLLAPYTKEEIELAIRQMFPTKALGPDG 662

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
           FP LFYQTY HVVGPKT+EACLN LNN  DI+ WN+TYIALIPK+KQP S+ DFRPISLC
Sbjct: 663 FPALFYQTYWHVVGPKTLEACLNALNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLC 685

Query: 181 NVSYKIISKSITN 194
           NVSYKIISKSITN
Sbjct: 723 NVSYKIISKSITN 685

BLAST of MS000208 vs. ExPASy TrEMBL
Match: A0A2N9FN47 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS16564 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 9.4e-43
Identity = 93/193 (48.19%), Postives = 130/193 (67.36%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           EIFWKQR R  WL  GD NT++FH+ A+ RK  N++H + + N     + + IE+  V Y
Sbjct: 771 EIFWKQRSRVAWLHGGDSNTRYFHECASQRKKTNTLHGLRDTNEVWQTDPDAIESIVVEY 830

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
           F ++F+SS+P++  I  +T L+  R+TPE+N  LL+P++ EEI++A+ QM P+K PGPDG
Sbjct: 831 FHNLFSSSNPTA--INEVTQLVDARVTPEMNSSLLSPFSSEEIKSALFQMSPSKAPGPDG 890

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
              LF+Q Y +VVG    EA L+ LN+   + + N T I LIPKVK P ++  FRPISLC
Sbjct: 891 MTALFFQKYWNVVGVHVTEAVLDCLNSGRLLGSINFTNIVLIPKVKAPVNMAHFRPISLC 950

Query: 181 NVSYKIISKSITN 194
           NV YKIISK + N
Sbjct: 951 NVIYKIISKVLVN 961

BLAST of MS000208 vs. ExPASy TrEMBL
Match: A0A2N9GWG4 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS31631 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 9.4e-43
Identity = 93/193 (48.19%), Postives = 130/193 (67.36%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           EIFWKQR R  WL  GD NT++FH+ A+ RK  N++H + + N     + + IE+  V Y
Sbjct: 771 EIFWKQRSRVAWLHGGDSNTRYFHECASQRKKTNTLHGLRDTNEVWQTDPDAIESIVVEY 830

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
           F ++F+SS+P++  I  +T L+  R+TPE+N  LL+P++ EEI++A+ QM P+K PGPDG
Sbjct: 831 FHNLFSSSNPTA--INEVTQLVDARVTPEMNSSLLSPFSSEEIKSALFQMSPSKAPGPDG 890

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
              LF+Q Y +VVG    EA L+ LN+   + + N T I LIPKVK P ++  FRPISLC
Sbjct: 891 MTALFFQKYWNVVGVHVTEAVLDCLNSGRLLGSINFTNIVLIPKVKAPVNMAHFRPISLC 950

Query: 181 NVSYKIISKSITN 194
           NV YKIISK + N
Sbjct: 951 NVIYKIISKVLVN 961

BLAST of MS000208 vs. ExPASy TrEMBL
Match: A0A2N9I335 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS48349 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.6e-42
Identity = 88/193 (45.60%), Postives = 123/193 (63.73%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           EI+W+QR R  W++ GD NTK+FH     R+  N I  + +  G    +  ++ N  V Y
Sbjct: 235 EIYWRQRSRVSWMREGDKNTKFFHAHCNHRREINLIKGLRDNEGVLQTDKIKMANIAVDY 294

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
           FQSIF+SS+P    I +  + +   +T E+N  LL  +N EE+  A+KQM+PTK PGPDG
Sbjct: 295 FQSIFSSSNPGDETINSCLDGLERVVTEEMNNMLLEDFNSEEVSQALKQMYPTKAPGPDG 354

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
              +FYQTY  +VGP+  +A L++L++   +   N T+IALIPKVK P  + DFRPISLC
Sbjct: 355 MSAVFYQTYWDIVGPEVTQAILSILHSGYMVNKINYTHIALIPKVKNPERITDFRPISLC 414

Query: 181 NVSYKIISKSITN 194
           NV YKI+SK + N
Sbjct: 415 NVIYKIVSKILAN 427

BLAST of MS000208 vs. ExPASy TrEMBL
Match: A0A2N9GPZ7 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS29430 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 2.1e-42
Identity = 89/193 (46.11%), Postives = 126/193 (65.28%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           EIFW+QR R  W+  GD NTK+FH +   R+  N I  + +++G    E  +I    V Y
Sbjct: 206 EIFWRQRSRVAWMSEGDKNTKFFHAQCNERRRTNHISGLRDRDGVWQTEKTKIAEIAVDY 265

Query: 61  FQSIFTSSSPSSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGPDG 120
           FQ IFTSS+PS+  I  +   + + +T  +N  L A + ++E+  A+KQM+PTK PGPDG
Sbjct: 266 FQGIFTSSNPSAESITTVLQGMESVVTNAMNDQLQAEFTKDEVSLALKQMYPTKAPGPDG 325

Query: 121 FPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPISLC 180
              +FYQTY  +VGP+  +A L++L++   +R  N T+IALIPKVK P ++ DFRPISLC
Sbjct: 326 MSAIFYQTYWDIVGPEVTQAILSILHSGYMLRKINYTHIALIPKVKNPENITDFRPISLC 385

Query: 181 NVSYKIISKSITN 194
           NV YKI+SK + N
Sbjct: 386 NVIYKIVSKVLAN 398

BLAST of MS000208 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 3.4e-21
Identity = 60/190 (31.58%), Postives = 93/190 (48.95%), Query Frame = 0

Query: 1   EIFWKQRFREDWLKWGDCNTKWFHKKATMRKSCNSIHVIENQNGCRTEELEEIENTFVAY 60
           E F++Q+ R  WL+ GD NT++FHK     ++ N I  +   +  R E + +++   VAY
Sbjct: 432 ESFYRQKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMDDDVRVENVTQVKEMIVAY 491

Query: 61  FQSIFTSSSP--SSSDIEAITNLIPTRITPEINVWLLAPYNQEEIENAIKQMFPTKTPGP 120
           +  +  S S   +   ++ I ++ P R    +   L A  + +EI  A+  M   K PGP
Sbjct: 492 YTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALPSDKEITAAVFAMPRNKAPGP 551

Query: 121 DGFPTLFYQTYCHVVGPKTVEACLNVLNNDGDIRNWNTTYIALIPKVKQPGSVFDFRPIS 180
           D F   F+     VV   T+ A          ++ +N T I LIPKV     +  FRP+S
Sbjct: 552 DSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVS 611

Query: 181 LCNVSYKIIS 189
            C V YKII+
Sbjct: 612 CCTVVYKIIT 621

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.13.9e-5158.03uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_006491472.14.2e-4547.42uncharacterized protein LOC102626455 [Citrus sinensis][more]
XP_024950112.11.6e-4447.15uncharacterized protein LOC112496847 [Citrus sinensis][more]
XP_042952177.16.6e-4346.84uncharacterized protein LOC122289261 [Carya illinoinensis][more]
XP_024038343.18.7e-4348.19uncharacterized protein LOC112097373 [Citrus clementina][more]
Match NameE-valueIdentityDescription
P113693.3e-1331.10LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P085489.1e-1130.41LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
O003701.0e-0928.82LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P143819.4e-0821.59Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1DX301.9e-5158.03uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A2N9FN479.4e-4348.19Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS16564 PE=4 SV=1[more]
A0A2N9GWG49.4e-4348.19Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS31631 PE=4 SV=1[more]
A0A2N9I3351.6e-4245.60Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9GPZ72.1e-4246.11Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
Match NameE-valueIdentityDescription
AT1G43760.13.4e-2131.58DNAse I-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR19446:SF440SUBFAMILY NOT NAMEDcoord: 13..193
NoneNo IPR availablePANTHERPTHR19446REVERSE TRANSCRIPTASEScoord: 13..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS000208.1MS000208.1mRNA