MS000194 (gene) Bitter gourd (TR) v1

Overview


Name	MS000194
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	EXPERA domain-containing protein
Location	scaffold251: 9436 .. 12956 (-)
RNA-Seq Expression	MS000194
Synteny	MS000194

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGGAAATCTGAAGCTGCTCCCCATCCCTACGTTCCCGCAGATCTGCAACTCTCCGAATATGTTCCCGTTTCACTCCCATTCTCCACCATTATCGGCGTCTATTTCCTCTCTTCCATCCTGGTCACTTCTCTCGTCTGGACCCTCTCCGGTATCTTCTCAACTTTCCTTTTTGTAATTCTTTAAACTCTGTTTTATGTTCATCAAACTCTGCTTGATTTCGTGCCGGAGAAGGAGATCAATAGTTTTCATAATCTCTTGTTGTATTGTTTTCGATCCCGTCTCCTTGATAGAGCTCATATGTTGAATTAATCGGAGGAAACCGAACAATTTTATGGATTCTTAAGCCTCTTGGGTATTCCTCTGGTAATTTTGATTTCATCTCGCCTATTGAATGTGCCGCCTGTCCCGAAGGCTGATGGTGAAACCGATGTTTTGGTCAACTCTGAGTTTCATTCAATTATTATTTCTGTTGTTTTTTCTTACGAGAAGATGCAGATCAATTAACGGATTCTTTTTTAGGACACCAGATTTCCTCTGTTTTATCTTGCTGTTCGTTGTGTGATTTGTGCTGCTGTTTTGGGAGGTGATTACTTTAGAATGGCTATCAGGGCGGAACAAATCTACTTTTCATTTTAACACGAAACACAAACTGTATTCTGACATGATGGCAAAATACGTTTCCTATGATTATTCTCTCTTGAAACGAAAGGCCATAAAAGTCTTCCTCTAAACAATTATTCTTCCATTCACTTTATACCAGATCAGGAACAGGAATGCTATTAGGATAAAATGTTATTGGCTGGTAAGTTTGTCTTTGTTGAGTGGTTATGAATAGAGTGCATTGGGGAGTGAGAGGGGCAGATTTTCTTGAGCAGTTTGGGAAGAGTACTAGGCATCTCGAAAAGCTTGGAATGTTGTAATTTTTGTCTAAAATTACTAGCATACTTCCCTTTAACTTCTTCATTTTTTGGTTCCTATCGAATTAGTATCACAACACCCGTTCTTGGAAATGGATCTTTTGAAGTCTTGGAGAATATATGAGAATATTGTTGAGAAGATGTTCAATGACTTAGGGTCCGTTTGATAACCATTTTATTTTTTGTTTTCTGGTTTTGAAAATTTAGCTTATAAGCATACTTTTTATCTCTGTGTTTCTTTTTTTTTCCTTTGCTTTTTTATTTTGTTATCTACCATATAAAAATATTTTCAAAATCTAAGCAAAATTTCGAAATTGAAAAAGTAGTATTTAAAATTTTTCTTAAAGGGAACTAAGAATTCAAATGTGTGCGTGAGAATAGTGAAAAAAATACCACAGAAATTGGGAAGTATGCATCATTTTTAAAAACAAAAAACTAAAAATGAAATTTTTAATAAATGGGGCTTAGCAATTCATGAGGGAGAAGAAATCTAAGGAGAATTTCAGTTTAGAAAAAATAAGGAGAATCAAAGGACGGTGAAAGAAGGAGAACGCAGAAGAAGCTGAATAGGAACAATCCAAAGTTGAAAGAAACGAAATTCGCACCTCAGGTAGTATTGTACAAACATTAGATCCTAACACCATCAATAAAGTTGGGAACACTTAACAATGGGTGATAAATTCAAACATTGTACGAAGGGTTGGAAGTATGGAGAGTCACAATTTCCAATGACAAACAACCAATTGTTATAGTGCAAATTCACGGGGAGAAATGAAGGGTTGTTGATTTCATAGAGTAGTAATGAGAGACTACTCAAGAGTTCGGTACTGTTTAATTTTATCTTTAATATCAATAGCATATCTTCATTAATTTGTACTTAAATCTATGGTTCATATCTTCTATTGGAAATGGATCTTGAGTCTTGGAGAATGTTGTTTGAAACATGCAATATGGCAAAGAAATTGTTAAATGATTTAGCACCCATGAGGGAGAAGAAAATTGACTACCGTCTCAGTAACTTTTCAGAAAGATCAAGAGGTTGATGGTGATTGCAGCATTAGGATAAAAGAAAAGGTTTGATGTTGGGGAGGAAAGCTTGGGTCTGGGCGTTTGTTTTTTCTTTAAAAGAAAGCATAGAAGAAGTTTCAAAACTTTGTGAACTTAAAACCTCTGGTTGGTGTCACATCCTTCCTTGTTCCGACCTTCTCGGCTCAGATCTCATACAACATAACCATTGTCTTTGCATAAAGTTGTAAAAATAGATTTTGAGGACTTATGTTGCAAGCTATGCTACATTTTGCTTTCTACTTCACTTTTTGTTCTTTTATTTTGCCTTTCATGTCGTATACTTGCTCTCTGCATCCTTATTCCTTATCTATTTGTTTTTCATTTGAGTTGGAATTTGAAATTATCAATGGAAAAAATGTATAGTTTCTTAGCATTGCAAGTATATTGTTAGAACCTCCAAATATATTGAAAGATAAAAGATAAACGAACTACAACAGCTACCAAGTATTAGAGGTACTTGGACCCTCCCCTCTTGAGATATCACTCAAGCCTAAAGCATACTCAATAATTGGATACCTTCATTGCTACACCCTCATTCTATTTATAACGAATCTCCCTAAAAACTTCCCTATCTAATTACTAAAGTCCCCTCGATACCATAATAATATCCCTTTATTACCCTAAGGGTCATTCCTATCCTATATTTGTTATTATTGTTTAGAATGGTTCCATACTTGCTGATTTTATCTTCATTTTCCCCTTATAACAGGACGACTTCCCAAAAAGGCTAAGATTGAGAGGTTGCTCATGTGCTGGTGGGTATTTACGGGCCTCACCCACATGATTTTAGAGGGTTATTTTGTTTTCTCTCCAGAATTTTACAAGGACAAGACTTCTTTCTACCTTGCTGAAGTTTGTAAGTCAACTTAATTCACTTTCTTTATAACCATAGTTGCTGTGGTAAGGAAAGAGCTGTGTGGATGTTCCTCTAATATCCCATGTACACAACTTTCCAGGGAAAGAATACAGCAAGGGAGATTCTCGATACGCTGCTAGAGAGTCGGGAATTGTGAGTATTGAAGGACTGACTTCCGTTTTGGAGGGTCCAGCGTGCATCTTAGCAATGTAAGATTTTATCATCTGAAATTTAGAAATGCATTGAAGGATTTACATTTGAAGTTTCTGTTTTGAATAGGTGTATTATATGTATTGTTTTGTCTCAAAAAAAAAAAAAATAAAATAAAATAAGAGGACATAGGTGTAGTATATGCTTCTGTTCTCTTCCTTTCTATTCCTTCAAAAATTTTGCTGGCCTGGAAAATTCGCAGGTATGCAATAGCAACAGAAAAGTCATACAGTTACATACTGCAGTTTGCTATATCATTGGGGCAGCTTTACGGGACTTTTTTGTACTACATAACTGCTGTGTTGGATGGTGACAACTTTTCTTCTGGCCCATTTTATTACTATGCATACTACGTAGTGGCAAATTCCTTCTGGATTCTTATTCCGTCGCTTATCGCCATACGGTGTTGGAAGAAAATTTGTGCAGCAATGAACAGTGAAGGCCACAAGAAAAGAAAAATTCGC

mRNA sequence

ATGGCGGGGAAATCTGAAGCTGCTCCCCATCCCTACGTTCCCGCAGATCTGCAACTCTCCGAATATGTTCCCGTTTCACTCCCATTCTCCACCATTATCGGCGTCTATTTCCTCTCTTCCATCCTGGTCACTTCTCTCGTCTGGACCCTCTCCGGACGACTTCCCAAAAAGGCTAAGATTGAGAGGTTGCTCATGTGCTGGTGGGTATTTACGGGCCTCACCCACATGATTTTAGAGGGTTATTTTGTTTTCTCTCCAGAATTTTACAAGGACAAGACTTCTTTCTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCTGCTAGAGAGTCGGGAATTGTGAGTATTGAAGGACTGACTTCCGTTTTGGAGGGTCCAGCGTGCATCTTAGCAATGTATGCAATAGCAACAGAAAAGTCATACAGTTACATACTGCAGTTTGCTATATCATTGGGGCAGCTTTACGGGACTTTTTTGTACTACATAACTGCTGTGTTGGATGGTGACAACTTTTCTTCTGGCCCATTTTATTACTATGCATACTACGTAGTGGCAAATTCCTTCTGGATTCTTATTCCGTCGCTTATCGCCATACGGTGTTGGAAGAAAATTTGTGCAGCAATGAACAGTGAAGGCCACAAGAAAAGAAAAATTCGC

Coding sequence (CDS)

ATGGCGGGGAAATCTGAAGCTGCTCCCCATCCCTACGTTCCCGCAGATCTGCAACTCTCCGAATATGTTCCCGTTTCACTCCCATTCTCCACCATTATCGGCGTCTATTTCCTCTCTTCCATCCTGGTCACTTCTCTCGTCTGGACCCTCTCCGGACGACTTCCCAAAAAGGCTAAGATTGAGAGGTTGCTCATGTGCTGGTGGGTATTTACGGGCCTCACCCACATGATTTTAGAGGGTTATTTTGTTTTCTCTCCAGAATTTTACAAGGACAAGACTTCTTTCTACCTTGCTGAAGTTTGGAAAGAATACAGCAAGGGAGATTCTCGATACGCTGCTAGAGAGTCGGGAATTGTGAGTATTGAAGGACTGACTTCCGTTTTGGAGGGTCCAGCGTGCATCTTAGCAATGTATGCAATAGCAACAGAAAAGTCATACAGTTACATACTGCAGTTTGCTATATCATTGGGGCAGCTTTACGGGACTTTTTTGTACTACATAACTGCTGTGTTGGATGGTGACAACTTTTCTTCTGGCCCATTTTATTACTATGCATACTACGTAGTGGCAAATTCCTTCTGGATTCTTATTCCGTCGCTTATCGCCATACGGTGTTGGAAGAAAATTTGTGCAGCAATGAACAGTGAAGGCCACAAGAAAAGAAAAATTCGC

Protein sequence

MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKIERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGPFYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR

Homology

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BLAST of MS000194 vs. NCBI nr
Match: XP_022158411.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Momordica charantia])

HSP 1 Score: 447.2 bits (1149), Expect = 8.3e-122
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI
Sbjct: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP
Sbjct: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR 225
           FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR
Sbjct: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR 224

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BLAST of MS000194 vs. NCBI nr
Match: XP_038881035.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida])

HSP 1 Score: 393.3 bits (1009), Expect = 1.4e-105
Identity = 191/223 (85.65%), Postives = 207/223 (92.83%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG+SEAA HPY+P DL+L EYVP+SLP STI+ VY LSS+ VT LVWTLSGRL K+AKI
Sbjct: 1   MAGESEAARHPYIPTDLKLPEYVPISLPVSTILSVYLLSSLFVTFLVWTLSGRLTKRAKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWW+FTGLTHM+LEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  ERLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           IEGLTSVLEGPACILA YAIATEKSYSYILQFAISLGQLYGTF+YYITA+LDGDNFSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIATEKSYSYILQFAISLGQLYGTFVYYITALLDGDNFSSGP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKI 224
            YYY+Y+VVANSFWILIPSLIA RCWKKIC AM++E H K K+
Sbjct: 181 IYYYSYFVVANSFWILIPSLIATRCWKKICIAMSTESHMKTKV 223

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BLAST of MS000194 vs. NCBI nr
Match: XP_004152168.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis sativus] >KGN52949.1 hypothetical protein Csa_015122 [Cucumis sativus])

HSP 1 Score: 384.0 bits (985), Expect = 8.7e-103
Identity = 188/222 (84.68%), Postives = 202/222 (90.99%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG SEAAPHPYVP DL+L +YVP+SLP STI+G Y L+S+ +  LVWTLSGRL KKAKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWW+FTGLTHM+LEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           IEGLTSVLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT LYY+TAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRK 223
           FYYYAYYVVANSFWILIPSLI IRCWKKIC AM+ E   K K
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

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BLAST of MS000194 vs. NCBI nr
Match: XP_008454161.1 (PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis melo])

HSP 1 Score: 378.3 bits (970), Expect = 4.8e-101
Identity = 185/222 (83.33%), Postives = 200/222 (90.09%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG SEAAPHPYVP DL+L +YVP+SLP STI+G Y LSS+ +  L+WTLSGRL KK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWW+FTGLTHM+LEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           IEGLTSVLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT LYYITAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRK 223
            YYY+YYVVANSFW+LIPSLIAIRCWKKIC AM+ E   K K
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTK 222

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BLAST of MS000194 vs. NCBI nr
Match: XP_022980063.1 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima])

HSP 1 Score: 375.2 bits (962), Expect = 4.0e-100
Identity = 182/223 (81.61%), Postives = 202/223 (90.58%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG+SEAA HPY P DL+L  YVP+SLP +TI+GVY LSS+ +T L+WTLSGRLPKK KI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWW+FTGLTH++LEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYAARES IVS
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYAARESAIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           +EGLT+VLEGPACILA+YAIAT+KSYSYILQFAISLGQLYGTF+YYITA+LDGDNFSS P
Sbjct: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKI 224
            YYY YYV+ NSFWILIPSLIAIRCWKKIC AM+SE HKK K+
Sbjct: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 221

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BLAST of MS000194 vs. ExPASy Swiss-Prot
Match: O48962 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thaliana OX=3702 GN=At1g20050 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 1.8e-79
Identity = 145/224 (64.73%), Postives = 178/224 (79.46%), Query Frame = 0


Query: 6   EAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKIERLLM 65
           E   HPYVP DL L  YVP+S+  S+I+ +Y  SS+LV SLVW L GR  KKAK+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLT 125
           CWW FTGLTH+ILEGYFVFSPEF+KD TS YLAEVWKEYSKGDSRY  R+S +VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 SVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGPFYYYA 185
           +V+ GPA +LA+YAIA EKSYSY+LQ AIS+ QLYG  +Y+ITA+L+GDNF++  FYYY+
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVVANSFWILIPSLIAIRCWKKICAAM-----NSEGHKKRKIR 225
           YY+ AN +W+LIPSLI+ RCWKKICAA      N E   K+K R
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

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BLAST of MS000194 vs. ExPASy Swiss-Prot
Match: Q9FTZ2 (Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0103600 PE=2 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.7e-64
Identity = 118/214 (55.14%), Postives = 159/214 (74.30%), Query Frame = 0


Query: 9   PHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKK-AKIERLLMCW 68
           PHPY PA+L L  +VP+ L  + I+  Y  +S+ +   VW +SGR  ++ +  +R LMCW
Sbjct: 6   PHPYAPAELHLPGFVPLQLSQAQILVPYLATSLFLLLAVWLISGRCSRRLSDTDRWLMCW 65

Query: 69  WVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTSV 128
           W FTGLTH+I+EG FVF+P F+ ++   Y  EVWKEYSKGDSRY AR+   V++EG+T+V
Sbjct: 66  WAFTGLTHIIIEGTFVFAPNFFSNQNPSYFDEVWKEYSKGDSRYVARDPATVTVEGITAV 125

Query: 129 LEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGPFYYYAYY 188
           LEGPA +LA+YAIA+ KSYS+ILQF + LGQLYG  +Y+ITA LDG NF + PFY++AY+
Sbjct: 126 LEGPASLLAVYAIASGKSYSHILQFTVCLGQLYGCLVYFITAYLDGFNFWTSPFYFWAYF 185

Query: 189 VVANSFWILIPSLIAIRCWKKICAAMNSEGHKKR 222
           + ANS W++IP++IAIR WKKICAA   E  K +
Sbjct: 186 IGANSSWVVIPTMIAIRSWKKICAAFQGEKVKTK 219

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BLAST of MS000194 vs. ExPASy Swiss-Prot
Match: Q9JJ46 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 GN=Ebp PE=1 SV=3)

HSP 1 Score: 134.0 bits (336), Expect = 2.0e-30
Identity = 75/222 (33.78%), Postives = 126/222 (56.76%), Query Frame = 0


Query: 10  HPYVPADLQLSEYVPVSLP-FSTIIGVYFLSSILVTSLVWTLSGRLP--KKAKIERLLMC 69
           HPY P  L+L  +VP  LP +  ++G++  S +L+  + W LS R+         RL +C
Sbjct: 9   HPYWPRHLRLDNFVPNDLPTWHILVGLFSFSGVLIV-ITWLLSSRVSVVPLGTGRRLALC 68

Query: 70  WWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLTS 129
           W+      H+++EG+F F  E   +  +F L+++WKEYSKGDSRY   +  IV +E +T+
Sbjct: 69  WFAVCTFIHLVIEGWFSFYHEILLEDQAF-LSQLWKEYSKGDSRYILSDGFIVCMESVTA 128

Query: 130 VLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSG----PFY 189
            L GP  +  + A    + + ++LQ  +S+GQ+YG  LY++T + DG  F  G    P Y
Sbjct: 129 CLWGPLSLWVVIAFLRHQPFRFVLQLVVSVGQIYGDVLYFLTELRDG--FQHGELGHPLY 188

Query: 190 YYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR 225
           ++ Y+V+ N+ W++IP ++     K +  A +   +K  KI+
Sbjct: 189 FWFYFVIMNAIWLVIPGILVFDAIKHLTNAQSMLDNKVMKIK 226

[Close]

BLAST of MS000194 vs. ExPASy Swiss-Prot
Match: P70245 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Ebp PE=1 SV=3)

HSP 1 Score: 128.3 bits (321), Expect = 1.1e-28
Identity = 75/222 (33.78%), Postives = 131/222 (59.01%), Query Frame = 0


Query: 10  HPYVPADLQLSEYVPVSLPFSTI-IGVYFLSSILVTSLVWTLSGR---LPKKAKIERLLM 69
           HPY P  L+L  +VP  LP S I +G++ +S  L+  + W LS R   +P  A   RL +
Sbjct: 9   HPYWPRHLKLDNFVPNDLPTSHILVGLFSISGGLIV-ITWLLSSRASVVPLGAG-RRLAL 68

Query: 70  CWWVFTGLTHMILEGYF-VFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGL 129
           CW+      H+++EG+F +++    +D+   +L+++WKEYSKGDSRY   +S +V +E +
Sbjct: 69  CWFAVCTFIHLVIEGWFSLYNGILLEDQA--FLSQLWKEYSKGDSRYILSDSFVVCMETV 128

Query: 130 TSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFS--SGPFY 189
           T+ L GP  +  + A   ++ + ++LQ  +S+GQ+YG  LY++T + +G        P Y
Sbjct: 129 TACLWGPLSLWVVIAFLRQQPFRFVLQLVVSMGQIYGDVLYFLTELHEGLQHGEIGHPVY 188

Query: 190 YYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR 225
           ++ Y+V  N+ W++IPS++ +   K + +A +    K  KI+
Sbjct: 189 FWFYFVFLNAVWLVIPSILVLDAIKHLTSAQSVLDSKVMKIK 226

[Close]

BLAST of MS000194 vs. ExPASy Swiss-Prot
Match: Q60490 (3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus OX=10141 GN=EBP PE=1 SV=3)

HSP 1 Score: 125.9 bits (315), Expect = 5.6e-28
Identity = 79/228 (34.65%), Postives = 118/228 (51.75%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGR--LPKKA 60
           MA  S    HPY P  L+L  +VP  L    I+ V F     +   +W LS R  +    
Sbjct: 1   MATTSTGPLHPYWPRHLRLDHFVPNDLSAWYIVTVLFTVFGALVVTMWLLSSRASVVPLG 60

Query: 61  KIERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGI 120
              RL +CW+      H+++EG+FV   +      +F L+++WKEY+KGDSRY   ++ I
Sbjct: 61  TWRRLSVCWFAVCAFVHLVIEGWFVLYQKAILGDQAF-LSQLWKEYAKGDSRYIIEDNFI 120

Query: 121 VSIEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSS 180
           + +E +T VL GP  + A+ A   +    Y+LQF ISLGQ+YG  LY++T   DG  F  
Sbjct: 121 ICMESITVVLWGPLSLWAVIAFLRQHPSRYVLQFVISLGQIYGDLLYFLTEYRDG--FQH 180

Query: 181 G----PFYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRK 223
           G    P Y++ Y+   N  W++IP ++     K+   A N+   K  K
Sbjct: 181 GEMGHPIYFWFYFFFMNVLWLVIPGVLFFDSVKQFYGAQNALDTKVMK 225

[Close]

BLAST of MS000194 vs. ExPASy TrEMBL
Match: A0A6J1E0U0 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charantia OX=3673 GN=LOC111024900 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.0e-122
Identity = 224/224 (100.00%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI
Sbjct: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP
Sbjct: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR 225
           FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR
Sbjct: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKIR 224

[Close]

BLAST of MS000194 vs. ExPASy TrEMBL
Match: A0A0A0KVP4 (EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.2e-103
Identity = 188/222 (84.68%), Postives = 202/222 (90.99%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG SEAAPHPYVP DL+L +YVP+SLP STI+G Y L+S+ +  LVWTLSGRL KKAKI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLTSLFLVLLVWTLSGRLTKKAKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWW+FTGLTHM+LEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           IEGLTSVLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT LYY+TAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYVTAVLDGDDFSSGP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRK 223
           FYYYAYYVVANSFWILIPSLI IRCWKKIC AM+ E   K K
Sbjct: 181 FYYYAYYVVANSFWILIPSLIGIRCWKKICIAMSIESQTKTK 222

[Close]

BLAST of MS000194 vs. ExPASy TrEMBL
Match: A0A1S3BY31 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=3656 GN=LOC103494647 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.3e-101
Identity = 185/222 (83.33%), Postives = 200/222 (90.09%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG SEAAPHPYVP DL+L +YVP+SLP STI+G Y LSS+ +  L+WTLSGRL KK KI
Sbjct: 1   MAGASEAAPHPYVPTDLKLPDYVPISLPLSTILGGYLLSSLFLVLLLWTLSGRLTKKTKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           +RLLMCWW+FTGLTHM+LEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIV 
Sbjct: 61  DRLLMCWWIFTGLTHMVLEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVG 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           IEGLTSVLEGPACILA YAIA+ KSYSYILQFAISLGQLYGT LYYITAVLDGD+FSSGP
Sbjct: 121 IEGLTSVLEGPACILAAYAIASGKSYSYILQFAISLGQLYGTLLYYITAVLDGDDFSSGP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRK 223
            YYY+YYVVANSFW+LIPSLIAIRCWKKIC AM+ E   K K
Sbjct: 181 IYYYSYYVVANSFWLLIPSLIAIRCWKKICNAMSIESQMKTK 222

[Close]

BLAST of MS000194 vs. ExPASy TrEMBL
Match: A0A6J1IY56 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima OX=3661 GN=LOC111479580 PE=3 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.9e-100
Identity = 182/223 (81.61%), Postives = 202/223 (90.58%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG+SEAA HPY P DL+L  YVP+SLP +TI+GVY LSS+ +T L+WTLSGRLPKK KI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYLLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWW+FTGLTH++LEGYFVFSPEFYKD  S YLAEVWKEYSKGDSRYAARES IVS
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKD--SSYLAEVWKEYSKGDSRYAARESAIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           +EGLT+VLEGPACILA+YAIAT+KSYSYILQFAISLGQLYGTF+YYITA+LDGDNFSS P
Sbjct: 121 LEGLTAVLEGPACILAVYAIATDKSYSYILQFAISLGQLYGTFMYYITALLDGDNFSSSP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKI 224
            YYY YYV+ NSFWILIPSLIAIRCWKKIC AM+SE HKK K+
Sbjct: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 221

[Close]

BLAST of MS000194 vs. ExPASy TrEMBL
Match: A0A6J1GU98 (probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata OX=3662 GN=LOC111457598 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.7e-99
Identity = 178/223 (79.82%), Postives = 201/223 (90.13%), Query Frame = 0


Query: 1   MAGKSEAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKI 60
           MAG+SEAA HPY P DL+L  YVP+SLP +TI+GVY LSS+ +T L+WTLSGRLPKK KI
Sbjct: 1   MAGESEAARHPYAPTDLELPGYVPISLPLTTILGVYVLSSLFITLLLWTLSGRLPKKTKI 60

Query: 61  ERLLMCWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVS 120
           ERLLMCWW+FTGLTH++LEGYFVFSPEFYKD +  YLAEVWKEYSKGDSRYA R+S IVS
Sbjct: 61  ERLLMCWWIFTGLTHIVLEGYFVFSPEFYKDGS--YLAEVWKEYSKGDSRYATRDSAIVS 120

Query: 121 IEGLTSVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGP 180
           +EGLT+VLEGPACILA+YAIAT+KSYSY+LQFAISLGQLYGTF+YYITA+LDGDNFSS P
Sbjct: 121 LEGLTAVLEGPACILAVYAIATDKSYSYVLQFAISLGQLYGTFMYYITALLDGDNFSSSP 180

Query: 181 FYYYAYYVVANSFWILIPSLIAIRCWKKICAAMNSEGHKKRKI 224
            YYY YYV+ NSFWILIPSLIAIRCWKKIC AM+SE HKK K+
Sbjct: 181 IYYYLYYVLLNSFWILIPSLIAIRCWKKICIAMSSESHKKTKV 221

[Close]

BLAST of MS000194 vs. TAIR 10
Match: AT1G20050.1 (C-8,7 sterol isomerase )

HSP 1 Score: 297.0 bits (759), Expect = 1.3e-80
Identity = 145/224 (64.73%), Postives = 178/224 (79.46%), Query Frame = 0


Query: 6   EAAPHPYVPADLQLSEYVPVSLPFSTIIGVYFLSSILVTSLVWTLSGRLPKKAKIERLLM 65
           E   HPYVP DL L  YVP+S+  S+I+ +Y  SS+LV SLVW L GR  KKAK+++LLM
Sbjct: 2   EELAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLM 61

Query: 66  CWWVFTGLTHMILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARESGIVSIEGLT 125
           CWW FTGLTH+ILEGYFVFSPEF+KD TS YLAEVWKEYSKGDSRY  R+S +VS+EG+T
Sbjct: 62  CWWTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGIT 121

Query: 126 SVLEGPACILAMYAIATEKSYSYILQFAISLGQLYGTFLYYITAVLDGDNFSSGPFYYYA 185
           +V+ GPA +LA+YAIA EKSYSY+LQ AIS+ QLYG  +Y+ITA+L+GDNF++  FYYY+
Sbjct: 122 AVIVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYS 181

Query: 186 YYVVANSFWILIPSLIAIRCWKKICAAM-----NSEGHKKRKIR 225
           YY+ AN +W+LIPSLI+ RCWKKICAA      N E   K+K R
Sbjct: 182 YYIGANCWWVLIPSLISFRCWKKICAAAAIANNNVETKTKKKTR 223

The following BLAST results are available for this feature:

BLAST of MS000194 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158411.1	8.3e-122	100.00	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Momordica charantia]	[more]
XP_038881035.1	1.4e-105	85.65	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Benincasa hispida]	[more]
XP_004152168.1	8.7e-103	84.68	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis sativus] >KG...	[more]
XP_008454161.1	4.8e-101	83.33	PREDICTED: probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucumis m...	[more]
XP_022980063.1	4.0e-100	81.61	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase [Cucurbita maxima]	[more]

BLAST of MS000194 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48962	1.8e-79	64.73	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Arabidopsis thalia...	[more]
Q9FTZ2	1.7e-64	55.14	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Oryza sativa subsp...	[more]
Q9JJ46	2.0e-30	33.78	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Rattus norvegicus OX=10116 ...	[more]
P70245	1.1e-28	33.78	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Mus musculus OX=10090 GN=Eb...	[more]
Q60490	5.6e-28	34.65	3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cavia porcellus OX=10141 GN...	[more]

BLAST of MS000194 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0U0	4.0e-122	100.00	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Momordica charanti...	[more]
A0A0A0KVP4	4.2e-103	84.68	EXPERA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G007050 PE=3...	[more]
A0A1S3BY31	2.3e-101	83.33	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucumis melo OX=36...	[more]
A0A6J1IY56	1.9e-100	81.61	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita maxima O...	[more]
A0A6J1GU98	3.7e-99	79.82	probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase OS=Cucurbita moschata...	[more]

BLAST of MS000194 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20050.1	1.3e-80	64.73	C-8,7 sterol isomerase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF05241	EBP	coord: 97..205 e-value: 1.5E-18 score: 67.2
None	No IPR available	PANTHER	PTHR14207:SF7	BNAANNG14740D PROTEIN	coord: 6..224
IPR007905	Emopamil-binding protein	PANTHER	PTHR14207	STEROL ISOMERASE	coord: 6..224
IPR033118	EXPERA domain	PROSITE	PS51751	EXPERA	coord: 60..202 score: 14.876264

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS000194.1	MS000194.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0047750	cholestenol delta-isomerase activity